Дисертації з теми "Functional mRNA"
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Gape, Helen. "Microheterogeneity of porcine calpastatin and its functional implications." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267624.
Повний текст джерелаWolf, Jana. "Structural and functional studies of mRNA PolyA tail processing complexes." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708435.
Повний текст джерелаRaher, Michael J. "Functional Analysis of Proteins Involved in Translational Regulation." Thesis, Boston College, 2003. http://hdl.handle.net/2345/433.
Повний текст джерелаCytoplasmic polyadenylation regulates translational activation of mRNA stored in immature Xenopus oocytes. This event is necessary for the beginning of oocyte maturation, and later for critical processes in early embryonic development. A major protein required for polyadenylation is the cytoplasmic polyadenylation element-binding protein (CPEB), which recruits a factor that promotes the interaction between Poly(A) polymerase and the end of the mRNA. Polyadenylation in turn leads to translation through interactions between CPEB and other proteins. Using a yeast two-hybrid screen, several of these proteins were identified and cloned, including two of note. X295, a zinc-finger containing novel protein, and DEK, which has significant homology with the Homo sapiens DEK involved in certain juvenile leukemias. Through the cloning of the genes encoding these proteins, transcription of mRNA, and protein overexpression in oocytes, a series of protein-protein interaction binding assays were performed. Immunoblotting of SDS-PAGE analyzed samples shows that GST-CPEB and HA-X295 interact in ovo, and suggests a possible in ovo interaction of endogenous CPEB and endogenous X295. In similar experiments, DEK and CPEB do not interact, suggesting they may not interact in ovo
Thesis (BS) — Boston College, 2003
Submitted to: Boston College. College of Arts and Sciences
Discipline: Biology
Discipline: College Honors Program
Fu, Xiaonan. "Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/96216.
Повний текст джерелаPHD
Henscheid, Kristy L. "Functional conservation and RNA binding of the pre-mRNA splicing factor U2AF65 /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1400950821&sid=5&Fmt=2&clientId=11238&RQT=309&VName=PQD.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 129-141). Also available for download via the World Wide Web; free to University of Oregon users.
Noerenberg, Marko. "Functional analysis of the KSHV ORF57 protein in mRNA nuclear export mechanisms." Thesis, University of Leeds, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574517.
Повний текст джерелаSampson, Natalie D. "Identification and functional characterisation of the novel pre-mRNA processing factor SCAF6." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272374.
Повний текст джерелаMuraru, Mariela I. "Functional analysis of Prp45p, a pre-mRNA splicing factor in Saccharomyces cerevisiae." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/15461.
Повний текст джерелаWills, Quintin Frank. "The genetics of miRNA and mRNA expression in human lymphoblastoid cell lines." Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.572601.
Повний текст джерелаSamsonova, Anastasiia. "Structural and functional insights into YB-1 and Lin28 interplay in mRNA regulation." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASL037.
Повний текст джерелаThe mRNA regulation in human cells is one of the key mechanisms allowing the cells to adapt to a new environment and to respond to incoming signals. In terms of protein synthesis, the regulation of mRNA translation is a preferable process for cells compared to a more rigid mechanism of transcription or degradation. The RNA-binding proteins (RBPs) play a key role in the mRNA translation regulation.In the present work, we made an effort to demonstrate that a human RBP containing a cold shock domain, Lin28a, can act in cooperation with another cold shock protein YB-1, a core protein of mRNPs. The interplay between two cold shock proteins is based on their high structure similarity, that potentially gives Lin28 an opportunity to regulate the mRNA target translation in a general way using YB 1 as an “entry badge” to the mRNP.To demonstrate the interplay between Lin28 and YB 1, several methods of structural and cellular biology were used in the present study. The oligomerization of Lin28-CSD and YB-1-CSD upon RNA binding was shown in vitro, and the amino acid residues responsible for that were highlighted by NMR spectroscopy. Then, the colocalization of Lin28 and YB-1 was demonstrated in cell cytoplasm. Also, the protein interplay was shown to have functional consequences, e.g. for cell proliferation and differentiation
Aibara, Shintaro. "Structural and functional characterization of the Mex67:Mtr2 complex in mRNA nuclear export." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708892.
Повний текст джерелаThomas, Anne L. "Structural and functional characteristics of novel mRNA isoforms of the death-promoting gene bax." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285747.
Повний текст джерелаSerdar, Lucas D. "The Functional Relationship between the Nonsense-Mediated mRNA Decay Pathway and the Prematurely Terminating Ribosome." Case Western Reserve University School of Graduate Studies / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1554304118763865.
Повний текст джерелаZielke, Barbara [Verfasser], and Thomas [Akademischer Betreuer] Stamminger. "The HCMV encoded mRNA-export factor pUL69 : functional conservation within the Betaherpesvirinae and identification of mRNA-targets during infection / Barbara Zielke. Betreuer: Thomas Stamminger." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2012. http://d-nb.info/1022737279/34.
Повний текст джерелаWorrell, Harrison. "CSPG4 in osteosarcoma : functional roles and therapeutic potential." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33146.
Повний текст джерелаLi, Kaiyong [Verfasser], and Bruno [Akademischer Betreuer] Kyewski. "Identification of a functional mRNA-miRNA network in mTEC development / Kaiyong Li ; Betreuer: Bruno Kyewski." Heidelberg : Universitätsbibliothek Heidelberg, 2018. http://d-nb.info/1177149249/34.
Повний текст джерелаCheah, Sern Yih. "Understanding the risk and functional importance of schizophrenia genetic factors." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/101340/1/Sern%20Yih_Cheah_Thesis.pdf.
Повний текст джерелаYue, Bai-Gong. "Regulation of adenovirus alternative pre-mRNA splicing : Functional characterization of exonic and intronic splicing enhancer elements." Doctoral thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-454.
Повний текст джерелаPre-mRNA splicing and alternative pre-mRNA splicing are key regulatory steps controlling geneexpression in higher eukaryotes. The work in this thesis was focused on a characterization of thesignificance of exonic and intronic splicing enhancer elements for pre-mRNA splicing.
Previous studies have shown that removal of introns with weak and regulated splice sitesrequire a splicing enhancer for activity. Here we extended these studies by demonstrating thattwo "strong" constitutively active introns, the adenovirus 52,55K and the Drosophila Ftzintrons, are absolutely dependent on a downstream splicing enhancer for activity in vitro.
Two types splicing enhancers were shown to perform redundant functions as activators ofSplicing. Thus, SR protein binding to an exonic splicing enhancer element or U1 snRNP bindingto a downstream 5'splice site independently stimulated upstream intron removal. The datafurther showed that a 5'splice site was more effective and more versatile in activating splicing.Collectively the data suggest that a U1 enhancer is the prototypical enhancer element activatingsplicing of constitutively active introns.
Adenovirus IIIa pre-mRNA splicing is enhanced more than 200-fold in infected extracts. Themajor enhancer element responsible for this activation was shown to consist of the IIIa branchsite/polypyrimidne tract region. It functions as a Janus element and blocks splicing in extractsfrom uninfected cells while functioning as a splicing enhancer in the context of infected extracts.
Phosphorylated SR proteins are essential for pre-mRNA splicing. Large amount recombinantSR proteins are needed in splicing studies. A novel expression system was developed to expressphosphorylated, soluble and functionally active ASF/SF2 in E. Coli.
Lau, Agatha. "RNA-binding of LC3 to the AU-rich element of fibronectin mRNA, a structural and functional study." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0003/MQ46043.pdf.
Повний текст джерелаJeridi, Aicha [Verfasser], and Vigo [Akademischer Betreuer] Heissmeyer. "Functional analysis of the mRNA decay factor Lsm1 in the immune system / Aicha Jeridi ; Betreuer: Vigo Heissmeyer." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1202011322/34.
Повний текст джерелаKiesler, Eva. "Isolation and functional characterization of Hrp65-binding proteins in Chironomus tentans." Doctoral thesis, Stockholm : Institutionen för molekylärbiologi och funktionsgenomik, Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-218.
Повний текст джерелаJin, Shao-Bo. "Molecular Cloning and Functional Characterization of Factors Involved in Post-transcriptional Gene Expression." Doctoral thesis, Stockholm : Institutionen för molekylärbiologi och funktionsgenomik, Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-24.
Повний текст джерелаYu, Yang. "REGULATION OF PRE-MRNA SPLICING IN MAMMALIAN CELLS: IDENTIFICATION AND CHARACTERIZATION OF INTRONIC AND EXONIC SILENCERS." Connect to text online, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1184182785.
Повний текст джерелаYounis, Shady. "Functional characterization of the biological significance of the ZBED6/ZC3H11A locus in placental mammals." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-329190.
Повний текст джерелаBach, Frauke [Verfasser], and Iris [Akademischer Betreuer] Bruchhaus. "Exploring the vacuolin gene family, mRNA production, localization and functional characterization in the Mycobacterium marinum - Dictyostelium discoideum model system / Frauke Bach. Betreuer: Iris Bruchhaus." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2014. http://d-nb.info/1053811187/34.
Повний текст джерелаSerrat, Farran Xènia 1993. "Unraveling the functional roles of sftb-1/SF3B1 and prpf-4/PRPF4B in Caenorhabditis elegans splicing and human disease." Doctoral thesis, Universitat Pompeu Fabra, 2019. http://hdl.handle.net/10803/668152.
Повний текст джерелаEl nematode Caenorhabditis elegans és un sistema experimental que pot ajudar a resoldre qüestions fonamentals en recerca biomèdica, incloses les conseqüències d’alteracions en el processament de pre-ARN missatgers o splicing. En aquest treball, hem aplicat les tècniques de CRISPR i seqüenciació d’ARN per a investigar les funcions de dues proteïnes involucrades en splicing, les quals s’han conservat al llarg de l’evolució i s’han associat a diverses malalties de formes diferents. Hem observat que mutacions en el gen sftb-1 relacionades amb càncer alteren el procés de splicing en C. elegans. Mitjançant l’ús de diferents al·lels, hem descobert noves interaccions de letalitat sintètica entre mutacions en el gen sftb-1 i alteracions addicionals en el complex U2 snRNP. El sistema té el potencial de ser utilitzat per a identificar altres interaccions amb diferents processos biològics. A més, hem humanitzat un domini de la proteïna SFTB-1 per sensibilitzar els cucs als fàrmacs pladienolide B i herboxidiene, que actuen com a moduladors de l’splicing. Finalment, hem iniciat la caracterització funcional de la quinasa reguladora del procés de splicing prpf-4 en aquest nematode mitjançant l’estudi de la seva localització subcel·lular dinàmica i del requeriment de la seva activitat quinasa durant el desenvolupament de C. elegans.
Galloway, Summer E. "Functional characterization of conserved domains within the L protein component of the vesicular stomatitis virus RNA-dependent RNA polymerase implications for transcription and MRNA processing /." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2010r/galloway.pdf.
Повний текст джерелаJansson, Anna M. "Targeting Infectious Disease : Structural and functional studies of proteins from two RNA viruses and Mycobacterium tuberculosis." Doctoral thesis, Uppsala universitet, Struktur- och molekylärbiologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-196623.
Повний текст джерелаRuppert, Ann-Kathrin [Verfasser], Peter [Akademischer Betreuer] Nürnberg, Peter [Akademischer Betreuer] Schneider, and Peter [Akademischer Betreuer] Kloppenburg. "Integrative functional genomic search for regulatory DNA sequence polymorphisms influencing DNA methylation and mRNA expression in hippocampal brain tissue / Ann-Kathrin Ruppert. Gutachter: Peter Nürnberg ; Peter Schneider ; Peter Kloppenburg." Köln : Universitäts- und Stadtbibliothek Köln, 2015. http://d-nb.info/1084872617/34.
Повний текст джерелаTejedor, Vaquero Juan Ramón 1984. "Systematic functional analyses of spliceosomal components reveal novel mechanisme of alternative splicing regulation." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/385718.
Повний текст джерелаEl procesamiento alternativo del pre-ARNm constituye uno de los pilares esenciales en la regulación de la expresión génica y expande la capacidad codificadora del genoma en organismos multicelulares. El Espliceosoma – la maquinaria encargada de la eliminación alternativa de los intrones- permite una regulación multifacética de los programas genéticos en el interior de la célula. El proceso de corte y empalme se sustenta en la interacción dinámica de cientos de componentes del Espliceosoma, y los distintos niveles de regulación de la compleja reacción de splicing permanecen aun sin descubrir. El objetivo principal de esta tesis se ha centrado en el desarrollo de tecnologías sistematicas de alto cribado para identificar reguladores potenciales del procesamiento alternativo del pre- ARNm, así como los mecanismos implicados en su regulación. Hemos identificado una gran variedad de reguladores del procesamiento alternativo de Fas/CD95, tanto componentes esenciales del espliceosoma como factores implicados en otros procesos biologicos, y hemos observado una conexión inédita entre la regulación del splicing alternativo y el proceso de homeostasis modulado por hierro. Mediante el uso de redes computacionales, hemos llevado a cabo un análisis sistemático y funcional de los componentes del Espliceosoma y hemos identificado el potencial regulador de los mismos en la reacción de corte y empalme. Nuestros resultados reflejan una inmensa plasticidad de los factores esenciales del Espliceosoma a lo largo de toda a reacción de ensamblaje. Además, hemos conseguido identificar el mecanismo potencial por el cual la homeostasis del hierro ejerce su función en splicing alternativo a través de la modulación de la actividad de unión a RNA -mediada por un dominio de unión a cinc- en la proteína reguladora de splicing SRSF7. Los resultados de esta tesis enfatizan la relevancia de las tecnologías emergentes de alto cribado y el análisis de redes computacionales en el estudio de complejos mecanismos moleculares, y desvelan nuevas conexiones funcionales entre la maquinaria de splicing y otros procesos celulares.
Hofmann, Katharina Bettina [Verfasser], Patrick [Akademischer Betreuer] Cramer, Patrick [Gutachter] Cramer, and Reinhard [Gutachter] Lührmann. "Transcriptome maps of general eukaryotic RNA degradation factors and identification and functional characterization of the novel mRNA modification N3-methylcytidine / Katharina Bettina Hofmann ; Gutachter: Patrick Cramer, Reinhard Lührmann ; Betreuer: Patrick Cramer." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1209738457/34.
Повний текст джерелаDiebold, Marie-Laure. "Etude biochimique, structurale et fonctionnelle du complexe chaperonne d'histone/facteur d'élongation Spt6/Iws1." Thesis, Strasbourg, 2012. http://www.theses.fr/2012STRAJ008/document.
Повний текст джерелаProduction of functional messenger RNA (mRNA) requires a complex mechanism that couples transcription with maturation and export of the mRNA. In addition to this mechanism, chromatin needs to be unwound to allow the transcription machinery access the DNA, this unwinding being also highly regulated. Thus, production of a functional mRNA requires a huge number of factors implicated in these different processes. Among these proteins Spt6 and Iws1 are participating in the mechanism of transcription, chromatin unwinding, and maturation and export of the mRNA. The work carried out during this thesis has enabled the biochemical, structural and functional characterization of these proteins, their complex and their interaction with other effectors of transcription. This work has specifically enabled the molecular and functional characterization (i) of the recruitment of Spt6 by RNA polymerase II and (ii) of the formation of the Spt6/Iws1 complex. Moreover, this work has identified putative new partners of Spt6, not ably the elongation factor TFIIS. Thus, our work has highlighted the essential and complex role of Spt6 and Iws1 during the production of functional mRNA, and has also enabled future studies of the complexes formed by these two proteins with other transcriptional factors
Chang, Chung-Te. "SRAG functions as a New mRNA export co-adaptor." Thesis, University of Sheffield, 2012. http://etheses.whiterose.ac.uk/2119/.
Повний текст джерелаChapman, Tajekesa Kudzaishe Pamacheche. "Regulation of PABP1 function by differential post-translational modification." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25875.
Повний текст джерелаCordiner, Ross Andrew Alex. "The cellular functions of the microprocessor complex." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25877.
Повний текст джерелаHe, Weihai. "SM-like proteins function in mRNA degradation of Saccharomyces cerevisiae." Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/279964.
Повний текст джерелаHill, Christopher G. "Studies in microrna function and gene dysregulation in ovarian cancer." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/53086.
Повний текст джерелаLombardi, Olivia. "Investigating the role of mRNA capping enzyme in C-MYC function." Thesis, University of Dundee, 2017. https://discovery.dundee.ac.uk/en/studentTheses/4816aeec-c481-4494-9a07-56e74a83c08e.
Повний текст джерелаHeimiller, Joseph Karl. "Genome-wide analysis of splicing requirements and function through mRNA profiling." Thesis, University of Colorado at Boulder, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3607314.
Повний текст джерелаThe RNA-binding proteins U2AF and PTB play important roles in gene expression in many eukaryotic species. Although U2AF and PTB have been well-studied, their functional requirements have not been investigated on a genome-wide scale. In this thesis, I analyze RNA expression data to determine the requirement of the general splicing factor U2AF in S. pombe and to identify genes misregulated in Drosophila PTB mutants. I find that many introns are insensitive to U2AF inactivation in a Schizosaccharomyces pombe U2AF59 mutant, prp2.1. Bioinformatics analysis indicates that U2AF-insensitive introns have stronger 5' splice sites and higher A/U composition. The importance of intronic nucleotide composition was further investigated using wild type RNA expression data sets. I show that nucleotide composition is a relatively important factor for regulated intron retention in a variety of species. I also analyzed the RNA-binding protein PTB using RNA Seq data to reveal genes misregulated in PTB mutants in D. melanogaster. I identify misregulation of alternative splicing in PTB mutants and putative PTB binding sites. In the PTB embryonic lethal mutant, which shows dorsoventral patterning defects, I show that dorsal fate genes are significantly up-regulated. I present a model to link PTB to dorsal closure defects. This thesis provides the first genome-wide analysis of U2AF in S. pombe and PTB in Drosophila melanogaster.
Hannigan, Molly M. "The Functions and Regulation of mRNA Processing During Male Germ Cell Development." Case Western Reserve University School of Graduate Studies / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1554291304563947.
Повний текст джерелаGrasso, Laura. "The regulation and function of mRNA cap methylation in pluripotency and differentiation." Thesis, University of Dundee, 2015. https://discovery.dundee.ac.uk/en/studentTheses/ea974122-f82d-4cca-afb2-9ea43c1587e4.
Повний текст джерелаHammarlöf, Disa L. "EF-Tu and RNase E : Essential and Functionally Connected Proteins." Doctoral thesis, Uppsala universitet, Mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159682.
Повний текст джерелаKiri, Arpna. "The isolation and function of the 3'untranslated region of the myosin heavy chain genes of skeletal muscle." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325611.
Повний текст джерелаTubeuf, Helene. "Développement de stratégies de criblage de mutations d'épissage dans des gènes de prédisposition au cancer. Demystifying the splicing code: new bioinformatics insights for the interpretation of genetic variants A staggering number of genetic variations affect the splicing pattern of BRCA2 exon 7: validation of the predictive power of splicing-dedicated silico analyses MLH1 exon 7, an emblematic exon sensitive to intronic mutations but not to alterations of exonic splicing regulators, sheds light into the performance of SRE-dedicated bioinformatics approaches Calibration of pathogenicity of partial splicing defects: The model of BRCA2 Exon 3." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR009.
Повний текст джерелаThe development of high-throughput DNA sequencing has greatly facilitated the screening of genetic variations within patient genome. Henceforth, one of the main challenges in medical genetics is no longer the detection of variations, but their functional and clinical interpretation. Recently, we showed by using splicing reporter minigene assays, that although splicing mutations, and in particular those affecting its regulation, are more prevalent than initially estimated, they could now be predicted by using dedicated bioinformatics tools. We thus extended the evaluation of the predictive power of these four newly developed computational approaches by a comparative study of the scores obtained by these approaches with experimental data for a total of about 1200 exonic variations. Our findings have demonstrated the reliability of these approaches, used alone or in combination, and allow to offer recommendations for their use as a filtration tool to prioritize the variations to be analysed as a priority in splicing-dedicated functional assays. Nevertheless, an exhaustive mutational analysis targeting MLH1 exon 7, has highlighted the apparent failure of these approaches, yet validated by studies focused on BRCA2 exon 7, MAPT exon 10 and MSH2 exon 5, suggesting that these methods might not be equivalently applicable to all exons and/or genes. Indeed, we have shown that this exon has particular characteristics, i.e. remarkably strong splice sites, conferring it a total resistance to splicing regulation mutations and defeating prediction tools. These findings help to better determine the limitations of these bioinformatics tools while contributing to their improvement. In spite of these advances, the pathogenicity assessment of splicing mutations remains complicated, especially of those leading to in-frame and/or partial splicing anomalies. By using variant-induced partial BRCA2 exon 3 skipping as a model system, we showed that BRCA2 tumor suppressor function tolerates a substantial reduction in expression level, as BRCA2 allele producing as much as 70% of transcript encoding deficient protein may not necessarily confer high-risk of developing cancer. Altogether, these data have important implications in the molecular diagnosis and clinical management of patients and their relatives, with a direct benefit for hereditary cancer-suspected families and should contribute to the interpretation of VSI identified by high throughput sequencing in any other genetic disease
Bhattacharyya, Debmalya. "Translation Modulation of Cellular mRNA by G-Quadruplex Structures." Kent State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=kent1469636863.
Повний текст джерелаBlasco, Moreno Bernat 1986. "Uncovering a novel function of the exonuclease Xrn1 in viral and cellular mRNA translation." Doctoral thesis, Universitat Pompeu Fabra, 2016. http://hdl.handle.net/10803/664207.
Повний текст джерелаXrn1 és una exonucleasa molt conservada entre espècies i que té un paper cabdal en la degradació de l’ARN missatger (ARNm) i la regulació de la transcripció. Emprant un sistema model basat en el virus del mosaic del brom (BMV) i el llevat Saccharomyces cerevisiae, en aquest treball es demostra que Xrn1 promou la traducció de l’ARN viral. Aquesta funció en traducció està lligada a la regió no traduïda a l’extrem 5’ i a la seqüència codificant. A més, mitjançant l’anàlisi amb perfils de polisomes s’observa que Xrn1 assisteix la iniciació de la traducció de l’ARN viral i que interacciona directament amb la maquinària de traducció. Tenint en compte que Xrn1 parcipa en la degradació i la transcripció d’ARNm, es van crear diferents mutants per tal d’analitzar la relació entre aquestes funcions i el rol de Xrn1 en traducció. En primer lloc, es va observar que l’acvitat exonucleasa pròpia de Xrn1 és necessària per tal que Xrn1 actuï en traducció. Expressant la exonucleasa nuclear (Rat1) al citoplasma de cèl·lules xrn1∆, s’aconsegueix compensar els defectes en degradació de l’ARN i en la taxa de creixement. En canvi, no es rescaten els defectes en la traducció de l’ARN viral. En segon lloc, ulitzant un mutant de Xrn1 que no pot ser importat al nucli (Xrn1∆NLS) es va concloure que la funció de Xrn1 en transcripció és independent de la funció en traducció. Tot seguit, mitjançant estudis amb perfil de ribosomes, es va constatar que Xrn1 també actua com a acvador de la traducció d’un subgrup d’ARNm cel·lulars. Aquests, codifiquen per funcions relacionades amb la glucosilació i estan enriquits en proteïnes del recle endoplasmàc. Tal i com s’observa per l’ARN de BMV, aquests ARNm cel·lulars depenen de l’acvitat exonucleasa de Xrn1 per a la seva traducció i tenen una elevada estructura secundària a la regió no traduïda de l’extrem 5’. En conjunt, els nostres resultats descriuen un nou exemple de la comunicació existent entre la degradació i la traducció de l’ARNm i revelen que Xrn1 té una funció inesperada en el control de la traducció de l’ARNm viral i cel·lular.
Swift, Robert. "Structure-function relationships in enzymes of the nucleotidyltransferase superfamily RNA ligation and mRNA capping /." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p3354742.
Повний текст джерелаTitle from first page of PDF file (viewed June 16, 2009). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 137-147).
Welch, Kasey C. "Expression Of Nicotinic Acetylcholine Receptor mRNA As a Function Of Age In Whole Hippocampus Preparations From Wistar Rats." Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2371.pdf.
Повний текст джерелаCenik, Can. "Studies on the Evolution and Function of Introns in 5' Untranslated Regions." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10020.
Повний текст джерелаNeu, Ancilla [Verfasser], and Remco [Akademischer Betreuer] Sprangers. "Structure, dynamics and function of proteins in the mRNA decay pathway / Ancilla Neu ; Betreuer: Remco Sprangers." Tübingen : Universitätsbibliothek Tübingen, 2016. http://d-nb.info/1165235986/34.
Повний текст джерела