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Статті в журналах з теми "Formalin-fixed, paraffin-embedded tissues- FFPE"
Obi, Ekenedirichukwu N., Daniel A. Tellock, Gabriel J. Thomas, and Timothy D. Veenstra. "Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics." Biomolecules 13, no. 1 (January 3, 2023): 96. http://dx.doi.org/10.3390/biom13010096.
Повний текст джерелаMagdeldin, Sameh, and Tadashi Yamamoto. "Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues." PROTEOMICS 12, no. 7 (April 2012): 1045–58. http://dx.doi.org/10.1002/pmic.201100550.
Повний текст джерелаDear, Jonathan D., Jane E. Sykes, and Danika L. Bannasch. "Quality of DNA extracted from formalin-fixed, paraffin-embedded canine tissues." Journal of Veterinary Diagnostic Investigation 32, no. 4 (June 9, 2020): 556–59. http://dx.doi.org/10.1177/1040638720929637.
Повний текст джерелаMichelsen, Nete V., Klaus Brusgaard, Qihua Tan, Mads Thomassen, Khalid Hussain, and Henrik T. Christesen. "Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray." ISRN Pathology 2011 (December 26, 2011): 1–12. http://dx.doi.org/10.5402/2011/275102.
Повний текст джерелаGuo, Tong, Weijie Wang, Paul A. Rudnick, Tao Song, Jie Li, Zhengping Zhuang, Robert J. Weil, Don L. DeVoe, Cheng S. Lee, and Brian M. Balgley. "Proteome Analysis of Microdissected Formalin-fixed and Paraffin-embedded Tissue Specimens." Journal of Histochemistry & Cytochemistry 55, no. 7 (March 19, 2007): 763–72. http://dx.doi.org/10.1369/jhc.7a7177.2007.
Повний текст джерелаŞEVİK, Murat. "The extraction of Peste Des Petits Ruminants Virus RNA from paraffin-embedded tissues using a modified extraction method." Journal of Advances in VetBio Science and Techniques 7, no. 2 (August 31, 2022): 202–9. http://dx.doi.org/10.31797/vetbio.1078235.
Повний текст джерелаSequeiros, Tamara, Marta García, Melania Montes, Mireia Oliván, Marina Rigau, Eva Colás, Inés de Torres, Juan Morote, Jaume Reventós, and Andreas Doll. "Molecular Markers for Prostate Cancer in Formalin-Fixed Paraffin-Embedded Tissues." BioMed Research International 2013 (2013): 1–15. http://dx.doi.org/10.1155/2013/283635.
Повний текст джерелаChung, Joon-Yong, Till Braunschweig, Reginald Williams, Natalie Guerrero, Karl M. Hoffmann, Mijung Kwon, Young K. Song, Steven K. Libutti, and Stephen M. Hewitt. "Factors in Tissue Handling and Processing That Impact RNA Obtained From Formalin-fixed, Paraffin-embedded Tissue." Journal of Histochemistry & Cytochemistry 56, no. 11 (July 21, 2008): 1033–42. http://dx.doi.org/10.1369/jhc.2008.951863.
Повний текст джерелаNucci, Ricardo, Wilson Jacob-Filho, Alexandre Busse, Laura Maifrino, and Romeu de Souza. "Anatomopathological Assessment of the Diaphragm in Formalin-Fixed, Paraffin-Embedded Sections." Journal of Morphological Sciences 35, no. 03 (September 2018): 173–76. http://dx.doi.org/10.1055/s-0038-1673611.
Повний текст джерелаHe, Helen, Yu Yang, Zhongmin Xiang, Lunyin Yu, Jouhara Chouitar, Jie Yu, Natalie Roy D’Amore, et al. "A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts." Journal of Biomarkers 2016 (May 10, 2016): 1–11. http://dx.doi.org/10.1155/2016/1274603.
Повний текст джерелаДисертації з теми "Formalin-fixed, paraffin-embedded tissues- FFPE"
Matilda, Rentoft. "The use of formalin fixed paraffin embedded tissue and global gene expression profiling for increased understanding of squamous cell carcinoma of the tongue." Doctoral thesis, Umeå universitet, Patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-54005.
Повний текст джерелаRossouw, Sophia Catherine. "Optimisation of proteomics techniques for archival tumour blocks of a South African cohort of colorectal cancer." University of Western Cape, 2020. http://hdl.handle.net/11394/8036.
Повний текст джерелаTumour-specific protein markers are usually present at elevated concentrations in patient biopsy tissue; therefore tumour tissue is an ideal biological material for studying cancer proteomics and biomarker discovery studies. To understand and elucidate cancer pathogenesis and its mechanisms at the molecular level, the collection and characterisation of a large number of individual patient tissue cohorts are required. Since most pathology institutes routinely preserve biopsy tissues by standardised methods of formalin fixation and paraffin embedment, these archived, FFPE tissues are important collections of pathology material, often accompanied by important metadata, such as patient medical history and treatments. FFPE tissue blocks are conveniently stored under ambient conditions for decades, while retaining cellular morphology due to the modifications induced by formalin.
2022
Djidja, M.-C., S. Francese, Paul M. Loadman, Chris W. Sutton, P. Scriven, E. Claude, M. F. Snel, J. Franck, M. Salzet, and M. R. Clench. "Detergent addition to trypsin digest and Ion Mobility Separation prior to MS/MS improves peptide yield and Protein Identification for in situ Proteomic Investigation of Frozen and FFPE Adenocarcinoma tissue sections." Wiley, 2009. http://hdl.handle.net/10454/4565.
Повний текст джерелаThe identification of proteins involved in tumour progression or which permit enhanced or novel therapeutic targeting is essential for cancer research. Direct MALDI analysis of tissue sections is rapidly demonstrating its potential for protein imaging and profiling in the investigation of a range of disease states including cancer. MALDI-mass spectrometry imaging (MALDI-MSI) has been used here for direct visualisation and in situ characterisation of proteins in breast tumour tissue section samples. Frozen MCF7 breast tumour xenograft and human formalin-fixed paraffin-embedded breast cancer tissue sections were used. An improved protocol for on-tissue trypsin digestion is described incorporating the use of a detergent, which increases the yield of tryptic peptides for both fresh frozen and formalin-fixed paraffin-embedded tumour tissue sections. A novel approach combining MALDI-MSI and ion mobility separation MALDI-tandem mass spectrometry imaging for improving the detection of low-abundance proteins that are difficult to detect by direct MALDI-MSI analysis is described. In situ protein identification was carried out directly from the tissue section by MALDI-MSI. Numerous protein signals were detected and some proteins including histone H3, H4 and Grp75 that were abundant in the tumour region were identified
Clift, Sarah J. "Standardization and validation of an immunoperoxidase test for African horsesickness virus using formalin-fixed, paraffin-embedded tissues." Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/24626.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2008.
Paraclinical Sciences
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Clift, Sarah Jane. "Standardization and validation of an immunoperoxidase test for African horsesickness virus using formalin-fixed, paraffin-embedded tissues." Pretoria : [s.n.], 2008. http://upetd.up.ac.za/thesis/available/etd-05132009-173308/.
Повний текст джерелаViljoen, Rabia. "Optimisation of sample preparation for DNA extraction from formalin fixed paraffin embedded tissues of unresolved sudden unexpected death cases." Master's thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33072.
Повний текст джерелаHutamo, Kutlwano Aggrineth. "Typing of Mycobacterium bovis in formalin-fixed, paraffin-embedded tissues from selected wildlife species in the Kruger National Park, South Africa." Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/29674.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2012.
Veterinary Tropical Diseases
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Lüder, Ripoli Florenza [Verfasser]. "Comparison of fresh frozen vs. formalin-fixed, paraffin-embedded specimens and the expression profiling of 16 target genes in neoplastic and non-neoplastic canine mammary tissues using a multiplex branched-DNA assay / Florenza Lüder Ripoli." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1125394560/34.
Повний текст джерелаKuo, Shanny Hsuan, and 郭. 軒. "Molecular Detection of Feline Coronaviruses in Formalin-Fixed and Paraffin-Embedded Tissue (FFPE) by nested RT-PCRs: a Diagnosis-Aiding Approach." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/dc943h.
Повний текст джерела國立臺灣大學
分子暨比較病理生物學研究所
105
Feline infectious peritonitis (FIP), caused by feline coronavirus (FCoV), is a lethal disease in cats. The clinical signs are non-specific and antemortem diagnosis remains challenging and frustrating. Appling histopathology combined with immunohistochemical (IHC) staining is considered as the gold standard for FIP diagnosis. However, the sensitivity of the IHC method depends much on the numbers of intralesional antigen-bearing cells. Due to the limitations of small sampling sizes as well as the equivocal IHC staining pattern in some specimens, formalin-fixed and paraffin-embedded tissue (FFPE) biopsies frequently submitted for histopathological examination for FIP are the most challenging specimens for pathologists. It has been demonstrated that the consensus PCR targeting 3’UTR alone is non-specific for diagnosis of FIP in fresh tissues. Moreover, two recently described mutations, the substitution of methionine (M) to leucine (L) amino acid mutation at position 1058 (M1058L) and the substitution of serine (S) to alanine (A) amino acid mutation at position 1060 (S1060A) in spike (S) gene, which together can distinguish feline infectious peritonitis virus (FIPV) from feline enteric coronavirus (FECV) in >95% of serotype I FCoV-infected cases in freshly-collected specimens, have suggested a potential diagnostic value. The aim of this study was to compare the uses of a consensus nested RT-PCR (nRT-PCR) targeting 3’UTR and a nRT-PCR targeting the two mutations in S gene in aiding the diagnosis of FIP in FFPE tissues. After evaluation of the RNA quality in FFPE tissues by a RT-PCR targeting the housekeeping gene of feline GAPDH, a total of 38 histopathologically and immunohistochemically confirmed FIP cases and 22 non-FIP cases were used as the source of RNA and examined nRT-PCRs. We have successfully extracted RNA and amplified FCoV genes in 31/38 (82%) FIP cases using consensus nRT-PCR, whereas 17/38 (42%) FIP cases were detected using the S-specific nRT-PCR. Following subsequent sequencing, 16 out of 17 serotype 1 cases had one of the two mutations (M1058L and S1060A) in the S gene. None of the FFPF tissues from these non-FIP cats were positive by both methods. We have demonstrated that in combined with histopathology and IHC staining, both consensus nRT-PCR and S-specific nRT-PCR were capable of detecting viral RNA from FFPE samples where IHC signals were equivocal and possibly misinterpreted as negativity. Both methods serve as a useful tool in supporting FIP diagnosis and for the retrospective study of FIP in archival FFPE tissues.
Zhang, XIAO. "EVALUATION OF RNA QUALITY FROM FORMALIN FIXED AND PARAFFIN EMBEDDED SAMPLES:APPLICATIONS AND LIMITATIONS." Thesis, 2008. http://hdl.handle.net/1974/1740.
Повний текст джерелаThesis (Master, Pathology & Molecular Medicine) -- Queen's University, 2008-09-26 10:49:50.044
Книги з теми "Formalin-fixed, paraffin-embedded tissues- FFPE"
Al-Mulla, Fahd, ed. Formalin-Fixed Paraffin-Embedded Tissues. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-055-3.
Повний текст джерелаFormalin-fixed paraffin-embedded tissues: Methods and protocols. New York: Humana Press, 2011.
Знайти повний текст джерелаFormalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols. Humana Press, 2016.
Знайти повний текст джерелаЧастини книг з теми "Formalin-fixed, paraffin-embedded tissues- FFPE"
Bonin, Serena, Patricia J. T. A. Groenen, Iris Halbwedl, and Helmut H. Popper. "DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues." In Guidelines for Molecular Analysis in Archive Tissues, 33–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_7.
Повний текст джерелаHowe, Karen. "Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues." In Methods in Molecular Biology, 17–24. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6524-3_3.
Повний текст джерелаLonguespée, Rémi, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw. "Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues." In Methods in Molecular Biology, 19–31. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7558-7_2.
Повний текст джерелаPiqueras, Marta, Manish Mani Subramaniam, Samuel Navarro, Nina Gale, and Rosa Noguera. "Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections." In Guidelines for Molecular Analysis in Archive Tissues, 225–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_34.
Повний текст джерелаTran, Diem, Mark Daniels, Ben Colson, Dikran Aivazian, Antonio Boccia, Ingrid Joseph, Steffan Ho, Steve French, Alex Buko, and Jing Wei. "Sample Preparation of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue for Proteomic Analyses." In Sample Preparation in Biological Mass Spectrometry, 159–70. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0828-0_10.
Повний текст джерелаAzimzadeh, Omid, Michael J. Atkinson, and Soile Tapio. "Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue." In Methods in Molecular Biology, 525–33. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1186-9_33.
Повний текст джерелаAzimzadeh, Omid, Michael J. Atkinson, and Soile Tapio. "Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue." In Methods in Molecular Biology, 109–15. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2550-6_10.
Повний текст джерелаBonin, Serena, Patricia J. T. A. Groenen, Iris Halbwedl, and Helmut H. Popper. "DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells)." In Guidelines for Molecular Analysis in Archive Tissues, 37–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_8.
Повний текст джерелаGomes, Bruno Costa, Bruno Santos, José Rueff, and António Sebastião Rodrigues. "Methods for Studying MicroRNA Expression and Their Targets in Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Tissues." In Methods in Molecular Biology, 189–205. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3347-1_11.
Повний текст джерелаDowling, Paul. "DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue." In Methods in Molecular Biology, 113–18. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2831-7_9.
Повний текст джерелаТези доповідей конференцій з теми "Formalin-fixed, paraffin-embedded tissues- FFPE"
Galindo, Hector, Luisa Solis, Junya Fujimoto, Nana E. Hanson, Christina McDowell, Erick Riquelme, XiMing Tang, et al. "Abstract 3195: Formalin-fixed and paraffin-embedded (FFPE) DNA recovery for high-throughput genotyping of lung cancer tissues." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3195.
Повний текст джерелаReinholz, Monica M., Debrah M. Thompson, Ihab Botros, Matt Rounseville, and Patrick C. Roche. "Abstract 1383: NGS-based measurement of gene expression of 2560 oncology-related biomarkers in formalin-fixed, paraffin-embedded (FFPE) tissues." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-1383.
Повний текст джерелаPalescandolo, Emanuele, Robert Jones, Alina Raza, Ashwini Sunkavalli, Priscilla K. Brastianos, Matthew Ducar, Christina Go, et al. "Abstract 3178: Can DNA from archived formalin-fixed paraffin embedded (FFPE) cancer tissues be used for somatic mutation analysis in next generation sequencing." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3178.
Повний текст джерелаEnglert, D., D. Wilson, and S. Laken. "Amplification of mRNA from Formalin-Fixed Paraffin-Embedded (FFPE) Breast Cancer Tissues without 3' Bias and Multiplex Expression Analysis on Flow-Through Microarrays." In Abstracts: Thirty-Second Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 10‐13, 2009; San Antonio, TX. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-09-3052.
Повний текст джерелаGu, Jian. "Abstract 4127: Expression profiling of paired formalin-fixed, paraffin-embedded (FFPE) and fresh-frozen tissue samples on Ion Torrent PGMTM." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4127.
Повний текст джерелаSmiley, Sheila, Celia Marginean, and Bryan Lo. "Abstract A40: Gene expression profiling of pancreatic cancer precursors directly from formalin fixed paraffin embedded (FFPE) tissue without nucleic acid extraction." In Abstracts: AACR Special Conference on Pancreatic Cancer: Advances in Science and Clinical Care; May 12-15, 2016; Orlando, FL. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.panca16-a40.
Повний текст джерелаThobe, Megan N., Suzane L. Um, Victoria L. Peek, Darryl W. Ballard, Bruce W. Konicek, Kelly M. Credille, Philip J. Ebert, Gregory P. Donoho, and S. Betty Yan. "Abstract 2087: Detection of KRAS mutations in circulating tumor cells (CTCs) and in formalin-fixed, paraffin-embedded (FFPE) tissue using castPCR method." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2087.
Повний текст джерелаBaumbach, L., J. Yan, M. Ahearn, C. Gomez, M. Jorda, T. Halsey, A. Mejias, et al. "Gene Expression Profiling of Formalin-Fixed, Pariffin-Embedded (FFPE) Tissues from Triple-Negative (TN) Breast Cancer (BC) Patients." In Abstracts: Thirty-Second Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 10‐13, 2009; San Antonio, TX. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-09-6125.
Повний текст джерелаNiland, Erin, Audrey D. McGuire, Mary L. Cox, and George E. Sandusky. "Abstract 3203: High quality DNA obtained with an automated DNA Extraction method with 15 to 40 year old formalin fixed paraffin embedded (FFPE) blocks from normal and cancer tissues." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3203.
Повний текст джерелаRusbuldt, Joshua James, Tanesha Cash-Mason, Shaozhou Tian, Alison VanSchoiack, Yan Liang, Chandra Rao, and Denis Smirnov. "Abstract 4691: Evaluation of the NanoString’s Digital Spatial Profiling (DSP) technology in formalin-fixed paraffin embedded (FFPE) cell line mixtures, PBMCs and non-small cell lung cancer (NSCLC) tissues." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-4691.
Повний текст джерела