Дисертації з теми "Forensic medicin"
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1
Tillmar, Andreas. "Populations and Statistics in Forensic Genetics." Doctoral thesis, Linköpings universitet, Institutionen för klinisk och experimentell medicin, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-54742.
Анотація:
DNA has become a powerful forensic tool for solving cases such as linking a suspect to a crime scene, resolving biological relationship issues and identifying disaster victims. Traditionally, DNA investigations mainly involve two steps; the establishment of DNA profiles from biological samples and the interpreta-tion of the evidential weight given by theses DNA profiles. This thesis deals with the latter, with focus on models for assessing the weight of evidence and the study of parameters affecting these probability figures. In order to calculate the correct representative weight of DNA evidence, prior knowledge about the DNA markers for a relevant population sample is required. Important properties that should be studied are, for example, how frequently certain DNA-variants (i.e. alleles) occur in the population, the differences in such frequencies between subpopulations, expected inheritance patterns of the DNA markers within a family and the forensic efficiency of the DNA markers in casework. In this thesis we aimed to study important population genetic parameters that influence the weight of evidence given by a DNA-analysis, as well as models for proper consideration of such parameters when calculating the weight of evi-dence in relationship testing. We have established a Swedish frequency database for mitochondrial DNA haplotypes and a haplotype frequency database for markers located on the X-chromosome. Furthermore, mtDNA haplotype frequencies were used to study the genetic variation within Sweden, and between Swedish and other European populations. No genetic substructure was found in Sweden, but strong similari-ties with other western European populations were observed. Genetic properties such as linkage and linkage disequilibrium could be im-portant when using X-chromosomal markers in relationship testing. This was true for the set of markers that we studied. In order to account for this, we pro-posed a model for how to take linkage and linkage disequilibrium into account when calculating the weight of evidence provided by X-chromosomal analysis. Finally, we investigated the risk of erroneous decisions when using DNA in-vestigations for family reunification. We showed that the risk is increased due to uncertainties regarding population allele frequencies, consanguinity and compet-ing close relationship between the tested individuals. Additional information and the use of a refined model for the alternative hypotheses reduced the risk of making erroneous decisions. In summary, as a result of the work on this thesis, we can use mitochondrial DNA and X-chromosome markers in order to resolve complex relationship in-vestigations. Moreover, the reliability of likelihood estimates has been increased by the development of models and the study of relevant parameters affecting probability calculations.
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Nilsson, Gunnel. "Stability of zopiclone in whole blood : Studies from a forensic perspective." Licentiate thesis, Linköping University, Linköping University, Clinical Pharmacology, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-58997.
Анотація:
Bio‐analytical results are influenced by in vivo factors like genetic, pharmacological and physiological conditions and in vitro factors like specimen composition, sample additives and storage conditions. The knowledge of stability of a drug and its major metabolites in biological matrices is very important in forensic cases for the interpretation of analytical results. Many drugs are unstable and undergo degradation during storage.
Zopiclone is a short‐acting hypnotic drug, introduced as a treatment for insomnia in the 1980s. However, this drug is also subject to abuse and can be found in samples from drug‐impaired drivers, recreational drug users and forensic autopsy cases. Zopiclone is analyzed in biological materials using different analytical methods. It is unstable in certain solvents and depending on storage conditions unstable in biological fluids. The aim of this thesis was to investigate the stability of zopiclone in human whole blood and to compare stability between authentic and spiked samples. Interpretation of zopiclone concentrations in whole blood is important in forensic toxicology. The following investigations were performed to study the stability of zopiclone in both spiked and authentic human blood.
First, different stability tests were performed. Spiked blood samples were stored at –20°C, 5°C and 20°C and the degradation of zopiclone was investigated in long‐ and short‐term stability. Authentic and spiked blood samples were stored at 5°C and differences in zopiclone stability were studied. Processed sample stability and effect of freeze/thaw cycles were also evaluated.
Second, influence of pre‐analytical conditions on the interpretation of zopiclone concentrations in whole blood was investigated. Nine volunteers participated in the study. Whole blood was obtained before and after oral administration of 2 x 5 mg Imovane®. Aliquots of authentic and spiked blood were stored under different conditions and zopiclone stability was evaluated. In this study, the influence from physiological factors such as drug interactions, matrix composition and plasma protein levels were minimized.
Analyses of zopiclone were performed by gas chromatography with nitrogen phosphorous detection and zopiclone concentrations were measured at selected time intervals. Degradation product of zopiclone was identified using liquid chromatography‐tandem mass spectrometry.
The first study showed that zopiclone degrades in human blood depending on time and temperature and may not be detected after long‐term storage. The degradation product 2‐amino‐5‐chloropyridine was identified following zopiclone degradation. The best storage condition was at –20°C even for short storage times, because freeze‐thaw had no influence on the results. In butyl acetate extracts, zopiclone was stable for at least two days when kept in the autosampler. However, in blood samples stored at 20°C a rapid decrease in concentration, was noticed. This rapid degradation at ambient temperature can cause an underestimation of the true concentration and consequently flaw the interpretation.
The second study showed no stability differences between authentic and spiked blood but confirmed the poor stability in whole blood at ambient temperature. The results showed that zopiclone was stable for less than 1 day at 20°C, less than 2 weeks at 5°C, but stable for 3 months at –20°C. This study, demonstrates the importance of controlling pre‐analytical conditions from sampling to analysis to avoid misinterpretation of toxicological results.
3
Zackrisson, Anna-Lena. "Pharmacogenetics from a Forensic Perspective : CYP2D6 and CYP2C19 genotype distributions in autopsy cases." Doctoral thesis, Linköpings universitet, Klinisk farmakologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-17936.
Анотація:
In Sweden about 550 individuals die every year due to drug intoxication. A challenge for the forensic toxicologist is to determine whether or not the analytical results can explain intoxication as a cause of death. The most common drugs found among intoxication cases are psychiatric drugs and analgesics. Many of these drugs are metabolised by CYP-enzymes such as CYP2D6 and CYP2C19. Genetic variations, polymorphisms, in the genes coding for these enzymes can lead to an inactive enzyme resulting in poor metabolism, which can lead to adverse drug reactions, even with fatal outcome. The CYP2D6 gene can be multiplied, which can lead to an ultra-rapid metabolism if the alleles are active. Another polymorphism, in the CYP2C19 gene, can also lead to an ultra-rapid metabolism. This increased metabolism can result in insufficient drug plasma concentration and, with that, failed treatment. Alternately, if the drug is a pro-drug and has to be activated by these enzymes, it can lead to a high amount of active metabolites. There is a large inter-individual variation of these polymorphisms and also a large variation between different populations. Additional information about an individual’s pharmacogenetics may possibly facilitate the interpretation of the postmortem result and contribute to solve the “toxicological puzzle”. The general aim of this thesis was to study if genetic variation in the drug metabolising enzymes, CYP2D6 and CYP2C19 can contribute to fatal intoxication. Reliable and rapid SNP and CNV assays suitable for forensic samples using PCR and pyrosequencing were developed for CYP2D6 and genotype frequencies in a Swedish population were shown to be in concordance with earlier published data. SNP assays were established for polymorphisms in the CYP2C19 gene. Genotype distributions in fatal intoxication cases were compared with Swedish blood donors and significant difference between the materials were established. The allele CYP2D6*4 was found to be less frequent among the intoxication cases, as compared with the blood donors. No differences in CYP2C19 genotype frequencies were found between the materials. These findings are the opposite of our hypothesis that we expected to find an increased number of individuals carrying genetic variations, leading to poor metabolism among fatal intoxication cases. However, we are convinced that information concerning an individual’s genotype can be of importance in specific intoxication cases. Further studies are required to illuminate this question. Two further autopsy materials were studied; suicide cases (intoxications excluded) and natural death cases. A significant increased number of individuals carrying more than two active CYP2D6 alleles among the suicide cases were found compared to natural death cases. Furthermore, we found some significant differences between the materials when the individuals in each material were grouped according to how many active CYP2D6 alleles they carry in combination with the CYP2C19 genotype, which was divided into six subgroups. We do not currently have any explanation for the differences between the materials.
4
Edlund, Hanna. "Sensitive Identification Tools in Forensic DNA Analysis." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-131904.
Анотація:
DNA as forensic evidence is valuable in criminal investigations. Implementation of new, sensitive and fast technologies is an important part of forensic genetic research. This thesis aims to evaluate new sensitive methods to apply in forensic DNA analysis including analysis of old skeletal remains. In Paper I and II, two novel systems for analysis of STRs, based on the Pyrosequencing technology, are presented. In Paper I, Y chromosomal STRs are analysed. Markers on the male specific Y chromosome are especially useful in analysis of DNA mixtures. In Paper II, ten autosomal STRs are genotyped. The systems are based on sequencing of STR loci instead of size determination of STR fragments as in routine analysis. This provides a higher resolution since sequence variants within the repeats can be detected. Determination of alleles is based on a termination recognition base. This is the base in the template strand that is excluded from the dispensation order in the sequencing of the complementary strand and therefore terminates the reaction. Furthermore, skeletal remains are often difficult to analyse, due to damaging effects from the surrounding environment on the DNA and the high risk of exogenous contamination. Analysis of mitochondrial DNA is useful on degraded samples and in Paper III, mtDNA analysis of 700 years old skeletal remains is performed to investigate a maternal relationship. The quantity and quality of DNA are essential in forensic genetics. In Paper IV the efficiency of DNA isolation is investigated. Soaking skeletal remains in bleach is efficient for decontamination but result in a lower DNA yield, especially on pulverised skull samples. In conclusion, this thesis presents novel sequencing systems for accurate and fast analysis of STR loci that can be useful in evaluation of new loci and database assembly as well as the utility of mtDNA in forensic genetics.
5
Stenberg, Stina. "Antisocial behaviors and substance abuse among mentally illoffenders - an undesirable role in time of treatment?" Thesis, Örebro universitet, Institutionen för medicinska vetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-93346.
Анотація:
Introduction Offenders who suffer from a severe mental illness are sentenced to forensic-psychiatric careinstead of imprisonment. A severe mental illness is a legal term determined by type anddegree of the psychiatric illness. Forensic-psychiatric care ends when signs of severe mentalillness is no longer present. Antisocial behaviors and mental illness due to substance abuse isnot regarded as severe by law but can arguably delay discharge from care. Aim To investigate whether time in treatment for offenders sentenced to compulsory forensicpsychiatriccare is prolonged by a propensity for substance abuse and antisocial behaviors. Methods Extended §7-evaluation dictums from 2017 have been reviewed. Data concerning substanceabuse and antisocial tendencies was assessed to assort offenders in two subgroups with lowestdegree contra highest degree of additional problematic behaviors. The two subgroups andtime in treatment collected through the Swedish National Forensic Psychiatric Register wascombined in a Kaplan-Meier estimator through SPSS. Results Median time in treatment for offenders with highest degree of additional problematic behavioras in substance abuse and or antisocial tendencies was 1048 days (range 1075). Since nodischarge has occurred, median time to discharge could not be estimated. Median time todischarge for offenders with lowest degree of additional problematic behavior was 760 days(range 532). Conclusions The study could not find time in treatment among severe mental ill offenders to be longer foroffenders with additional substance abuse and or antisocial characteristics of crime in relationto offenders without.
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Berg, Sascha, and Maria Pakou. "Delaktighet i rättspsykiatrisk vård : En kulturanalys av meningsskapande sammanhang." Thesis, Linköpings universitet, Avdelningen för omvårdnad och reproduktiv hälsa, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-171444.
Анотація:
Bakgrund: Begreppet personcentrerad har internationellt blivit vedertaget inom omvårdnadsvetenskap och används för att beskriva en vårdform där vården utgår från ett partnerskap med patienten. Engagemang, trygghet, tillit, att vara delaktig i planeringen av vården och en vårdande relation upplevs vara viktiga komponenter som främjar delaktigheten. För att öka förståelsen om delaktighetens och personcentreringens syfte inom den rättspsykiatriska vårdkontexten krävs studier som beskriver de mångfacetterade begreppen ur vårdarens och patientens perspektiv. Syfte: Att förstå upplevelser av delaktighet och personcentrerad vård hos patienter och personal på en rättspsykiatrisk avdelning och diskutera hur delaktighet kan möjliggöras. Design: Studien är en kulturanalys och ansatsen är hermeneutisk. Metod: Enskilda halvstrukturerade intervjuer med fyra vårdare och fyra patienter på en rättspsykiatrisk avdelning i Mellansverige genomfördes. Intervjuerna har bearbetats utifrån en kulturanalytisk hermeneutisk arbetsmodell i sju faser. Interna dokument från sjukhuset analyserades avseende delaktighet. Resultat: Oklarheter gällande vårdprocessen och rättspsykiatrins strukturer som är inte fullkomligt tydliga inverkar på upplevelsen av delaktighet. Konklusion: Personal och patienter har olika uppfattningar om strukturernas funktion. Personalen anser att patienternas delaktighet är en förutsättning för vårdprocessen. Patienter uttrycker osäkerhet kring vad som förväntas av dem. Identitet och tolkning av den egna rollen kan påverka delaktigheten hos patienter inom rättspsykiatri.Background: The concept of person-centered care is internationally accepted in nursing science and describes a form of care with an active patient included in all aspects of care. Commitment, trust, and involvement in the planning of care and a caring relationship are perceived to promote participation. To increase understanding person-centering in the forensic psychiatric care context, studies on the multifaceted concepts from carers’ and patients’ perspectives are required. Aim: To understand experiences of participation and person-centered care among patients and staff in a forensic psychiatric ward and discuss how participation can be promoted. Method: Individual semi-structured interviews with four carers and four patients in a forensic psychiatric hospital in Central Sweden were conducted. A seven-phase cultural analysis model was used. Internal documents were analyzed for statements about participation. Design: The study is a cultural analysis with a hermeneutic approach. Result: Unclear care process and structures of forensic care affect the experience of participation. Conclusion: The perceptions of the function of the structures differ. Staff believe that patient participation is a prerequisite for the care process. Patients express uncertainty about what is expected of them. Identity and interpretation of one's own role can influence the participation of patients in forensic psychiatry.
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Cerigatto, Wanderley [UNESP]. "Análise faunística de dípteros necrófagos: ecologia e aplicação forense." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/87814.
Анотація:
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cerigatto_w_me_botib.pdf: 598768 bytes, checksum: 0ae452c31f7ad0b15c41e8b607d6e724 (MD5)A Entomologia Forense é a ciência que aplica o estudo dos insetos a procedimentos legais. As pesquisas nesta área são feitas desde 1850 e nas últimas décadas vêm obtendo progressos. Esse trabalho visa constituir um banco de dados regional que possa servir como referencial científico nas investigações criminais e estimativas pós-morte, como também no estudo de casos reais que chegaram até o NPML (Núcleo de Perícias Médico-legais) Bauru, Estado de São Paulo. Análises faunísticas foram realizadas em 23 corpos, que foram conduzidos até o NPML. Os corpos eram de vítimas que sofreram morte violenta ou morte natural, para investigação da causa morte, atendendo a solicitação de autoridade competente. Na análise realizada foram encontradas duas famílias de dípteros necrófagos: Calliphoridae e Sarcophagidae. Dentre os exemplares de dípteros coletados da família Calliphoridae a espécie mais abundante foi Chrysomya albiceps (49,30%), seguida, por C. megacephala (30,56%), e Lucilia eximia (8,13%). Além destas espécies, foram também encontradas Lucilia cuprina (5,69%), Cochliomyia macellaria (1,45%) e Lucilia sericata (1,21%). Espécimes da família Sarcophagidae totalizaram 3,66% dos insetos coletados. Os vinte e três corpos analisados constituíram-se em 23 casos detalhadamente investigados, associando a presença dos insetos com as informações dos laudos necroscópicos
Forensic entomology is the science, which applies insects for legal proceedings. Researches in this area have been performed since 1850 and in the last years researchers have obtained success. In this study a regional data set is presented to be used as a scientific reference in criminal investigations and post mortem interval estimates, as well as to be employed in real cases from the NPML (Núcleo de Perícias Médico-legais) of Bauru, São Paulo. Fauna analysis was performed in 23 corpses, which were led to the NPML. Corpses were victims, which have suffered violent or natural death, separated to investigate the death cause in response to requirements by authorities. In the analysis performed two families of necrophagous dipterans were found, Calliphoridae and Sarcophagidae. The most abundant Calliphoridae species was Chrysomya albiceps (49.30%), followed by C. megacephala (30,56%) and Lucilia eximia (8.13%). Other species were also found such as Lucilia cuprina (5.69%), Cochliomyia macellaria(1 ,45%) and Lucilia sericata (1.12%). The percentage of Sarcophagidae individuais was 3.66%. The twenty-three corpses analyzed resulted in 23 cases investigated in detail, associating the presence of insects with death data
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Theodoro, Matheus João Accoroni. "Aplicação da computação gráfica na reconstrução de face para reconhecimento: um estudo de caso." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-15052012-101538/.
Анотація:
A reconstrução facial, também conhecida como aproximação facial, é conhecida e realizada há muito tempo por profissionais da medicina forense e de áreas antropológicas. No caso da medicina forense, por exemplo, ela pode ser bastante útil quando não existem indícios que viabilizem a identificação do individuo por meios considerados de maior precisão, como por exemplo, exame de DNA ou de arcos dentários. Outra aplicação possível é auxiliar aos investigadores na redução do número de sujeitos (soluções) possíveis em casos de indivíduos não identificados, viabilizando a aplicação de exames de maior precisão. Existem vários métodos utilizados para a reconstrução facial, que pesquisadores constantemente buscam melhorar e aperfeiçoar. Esses métodos podem ser classificados em reconstrução facial por meio de imagens 2D ou reconstrução facial por meio de objetos 3D. Dentre esses métodos, podemos encontrar a reconstrução facial que faz uso de ferramentas de computação gráfica em 3D que pode ser realizada tanto na forma manual, onde ferramentas de computação gráfica simulam ferramentas utilizadas no mundo real no processo de reconstrução facial, ou na forma automatizada, onde o usuário informa ao software alguns dados como sexo, idade e etnia e a máquina realiza todo o processo de reconstrução facial. A proposta desse trabalho foi de aplicar técnicas de modelagem e de inserção de medidas de tecidos moles em um modelo 3D de crânio, de forma a validar o uso da computação gráfica como ferramenta de auxilio ao reconhecimento facial em procedimentos de medicina forense. O modelo de crânio foi reconstruído a partir de imagens obtidas por exame de tomografia computadorizada de um crânio real. Foi realizado um estudo de caso de um cadáver exumado de uma pessoa que possuía o registro de imagem em vida e utilizados dados de medidas de tecidos moles da face de brasileiros. Os testes foram realizados manualmente utilizando ferramentas de computação gráfica em 3D, atualmente disponíveis e os resultados avaliados de forma quantitativa. Os testes quantitativos foram realizados de forma a comparar os relevos 3D das reconstruções de face com interpolação e sem interpolação de pontos craniométricos e para diferentes padrões de tecidos moles.The facial reconstruction, also known as facial approximation, is performed for centuries by forensic medicine professionals and anthropological areas researchers. In the forensic medicine, for example, it is used when there is no evidence that allows the identification of the individual by considered accurate means, such as DNA testing or dental records. Thus, the purpose of facial reconstruction is to assist researchers in reducing the number of possible unidentified people, therewith allowing the use of accurate tests. There are several methods used for facial reconstruction and researchers are constantly seeking to refine and improve. Then, these methods can be classified as facial reconstruction using 2D images or 3D objects. Among these methods, we can find the facial reconstruction that uses 3D computer graphics tools. This method can be performed both in manual form, where computer graphics tools are used in simulating the real world tools in the facial reconstruction process, or the automated way, where the user input data such as gender, age and ethnicity and the machine performs the entire process of facial reconstruction. The work purpose is to apply modeling techniques and the inclusion of soft tissues measures in 3D skull model, to validate the computer graphics use as a tool to aid the facial recognition in forensic medicine procedures. The skull model was reconstructed from computed tomography. Thus, we performed a study case of an exhumed body, who had the image registration in life as well as soft tissue measures data of the Brazilians faces. The tests were performed using manually 3D computer graphics tools currently available and the results were evaluated both quantitatively. The quantitative tests were performed comparing the surfaces of 3D reconstructions with interpolated and no interpolated craniometric points and to different soft tissues patterns.
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Dias, Inês Isabel Marques Lourinhã. "Caracterização e prevalência de xenobióticos em vítimas fatais sujeitas a autópsia na Delegação do Norte do Instituto Nacional de Medicina Legal, I.P de 2007 a 2008." Master's thesis, Faculdade de Medicina da Universidade do Porto, 2011. http://hdl.handle.net/10216/62214.
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Dias, Inês Isabel Marques Lourinhã. "Caracterização e prevalência de xenobióticos em vítimas fatais sujeitas a autópsia na Delegação do Norte do Instituto Nacional de Medicina Legal, I.P de 2007 a 2008." Dissertação, Faculdade de Medicina da Universidade do Porto, 2011. http://hdl.handle.net/10216/62214.
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Kruse, Kristina [Verfasser], Norbert [Akademischer Betreuer] Leygraf, and Jens [Akademischer Betreuer] Wiltfang. "Schizophrene Patienten der forensischen Psychiatrie im Vergleich zu schizophrenen Patienten der Allgemeinpsychiatrie / Kristina Kruse geb. Piontek. Gutachter: Jens Wiltfang. Betreuer: Norbert Leygraf." Duisburg, 2012. http://forensik-essen.de.
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Crawford, C. "The emergence of English forensic medicine : Medical evidence in common-law courts, 1730-1830." Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381838.
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Lukhozi, Sipho Michael. "Dual obligations in clinical forensic medicine." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86537.
Анотація:
Thesis (MPhil)--Stellenbosch University, 2014.ENGLISH ABSTRACT: This thesis discusses ethical dilemmas faced by district surgeons in South Africa. District surgeons render clinical forensic services, which means that they deal mainly with detainees and victims of crime. The main functions of district surgeons are the collection of forensic evidence from patients and the care of detainees. So the focus is to assist in the administration of justice rather than improvement of patient wellbeing. The district surgeon may therefore find himself in a situation where patients’ interests are in conflict with those of law enforcement agencies. Being a medical practitioner in clinical forensic medicine, the district surgeon has an obligation to assist in the administration of justice, as opposed to the traditional obligation to care for patients and put patient’s interests first. This allegiance to both administration of justice as well as patient wellbeing lead to an ethical dilemma of dual loyalties. A dual obligations presents an ethical dilemma for the district surgeon, especially if they are in conflict and mutually exclusive. I discuss the detention and subsequent death of Steve Biko to illustrate how dual obligations can lead to serious human rights violations and even death. Dual obligations are however not limited to detainees and police custody settings, and I demonstrate this by discussing three other scenarios commonly encountered by district surgeons. There is a lack clear guidance for district surgeons who are faced with a conflict of obligations. I explore several ethical theories including consequentialism, deontology and virtue ethics, in search of an ethical framework suitable for resolving conflicts in clinical forensic medicine. I therefore argue that a duty based ethical framework is central to clinical forensic medicine and the resolution of loyalty conflicts. I recommend the resolution of conflicts by using an approach developed by Benjamin (2006). This approach involves weighing -up the different duties in conflict, applying philosophical reasoning and then amelioration. By adopting a structured and wellreasoned ethical framework, district surgeons will be able to deal with conflicts of obligations better.
AFRIKAANSE OPSOMMING: Hierdie tesis bespreek etiese dilemmas wat in die gesig gestaar word deur distriksgeneeshere in Suid-Afrika. Distriksgeneeshere lewer kliniese forensiese dienste, wat beteken dat hulle handel hoofsaaklik oor die gevangenes en slagoffers van misdaad. Die belangrikste funksies van distriksgeneeshere is die insameling van forensiese getuienis van pasiënte, en die sorg van gevangenes. Met hierdie benadering is die fokus om te help met die administratiewe doeleindes van geregtigheid, eerder as die verbetering van die pasiënt se welstand. Die distriksgeneesheer kan hom dus in 'n situasie vind waarby die pasiënte se belange in konflik is met dié van wetstoepassingsagentskappe. As 'n geneesheer in kliniese forensiese geneeskunde, het die distriksgeneesheer 'n verpligting om te help met die administrasie van geregtigheid, in teenstelling met die tradisionele verpligting om te sorg vir hul pasiënte, en hul welstand eerste te plaas. Hierdie getrouheid gaan gepaard met beide regspleging, sowel as die welstand van die pasiënt, wat kan lei tot 'n etiese dilemma van dubbele lojaliteit. Dubbele verpligtinge bied 'n etiese dilemma vir die distriksgeneesheer, veral as hulle in konflik en wedersyds uitsluitend is. Ek bespreek die aanhouding en die daaropvolgende dood van Steve Biko om te illustreer hoe dubbele verpligtinge kan lei tot ernstige skending van menseregte en selfs die dood. Dubbele verpligtinge is egter nie beperk tot die gevangenes en polisie-aanhouding instellings nie, en ek demonstreer dit deur die bespreking van drie ander “scenario's” wat oor die algemeen eervaar word deur distriksgeneeshere. Daar is 'n gebrek aan duidelike riglyne vir distriksgeneeshere wat 'n botsing van verpligtinge in die gesig staar. Ek verken verskeie etiese teorieë insluitende konsekwensialisme, deontologie en deugde-etiek, op soek na 'n etiese raamwerk geskik vir die oplossing van konflikte in kliniese geregtelike geneeskunde. Ek argumenteer dus dat 'n pligsgebaseerde etiese raamwerk sentraal is tot kliniese forensiese geneeskunde, en die resolusie van lojaliteit konflikte. Ek beveel die oplossing van konflikte deur die gebruik van 'n benadering wat ontwikkel is deur Benjamin (2006). Hierdie benadering behels 'n gewigsoorweging tussen die verskillende pligte in konflik, die toepassing van filosofiese redenasie en verbetering. Deur die aanneming van 'n gestruktureerde en beredeneerde etiese raamwerk, sal distriksgeneeshere dus in staat wees om konflikte van verpligtinge beter te hanteer.
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Duvall, Nicholas. "Forensic medicine in Scotland, 1914-39." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/forensic-medicine-in-scotland-191439(7ca3afe7-7887-4133-9ba0-5206f2ea2237).html.
Анотація:
This thesis examines the practice of forensic medicine in Scotland in the period 1914 to 1939. This was a time of significant dynamism for the discipline, in which it enjoyed a high public profile and played an important role in the investigation of crime. The project focuses in particular on medico-legal practice at an elite level, based in specialist departments in the universities of Edinburgh and Glasgow. As well as producing a significant amount of research and textbook material, and thus constituting authorities within the discipline, representatives of these institutions gave expert evidence in a number of high-profile trials. Thus, an examination of their work can show how medico-legal knowledge was constructed, presented and challenged. To this end, four main areas of forensic medical practice are analysed, including the post-mortem examination, the laboratory analysis of trace evidence, the investigation of shootings and the use of photography. The development of the techniques contained within these categories is charted, as is the range of situations to which they were applied and the various ways in which their use was challenged in court by hostile legal counsel. Sources including textbooks and journal articles, medical case reports, photograph albums and trial transcripts are used. A fifth section explores an area of the public face of the discipline, specifically the popular output of two of its most famous practitioners, Sydney Smith and John Glaister Jr. Both produced memoirs and newspaper serials after retirement. These are used to explore the ways they reflected on their careers and spun their legacies, portraying themselves as impartial servants of science and justice. The thesis argues that the place of forensic medicine in wider institutional, investigative and geographical networks was central to its existence. The discipline collaborated extensively, both with representatives of other areas of the medical profession and with external authorities, professions and trades. Means of communication, such as written reports and samples taken at autopsy, allowed experts in the universities to lend their expertise to the non-specialists in peripheries by providing expert opinions based on materials sent to them. The scrutiny of post-mortem reports produced by peripheral generalists allowed medico-legists’ expertise to be spread over a wide geographical area. The thesis also reflects on the ways in which medico-legists guarded against error. Techniques derived from other areas of medicine and science were not adopted for use in court until their reliability could be demonstrated satisfactorily, and controls and standards were built in to procedures.
15
Hernández, J., R. Guillén, F. Peña, C. Rojas, Y. Yonz, and G. Zavala. "Medicina forense." Universidad Peruana de Ciencias Aplicadas - UPC, 2009. http://hdl.handle.net/10757/272500.
16
Cerigatto, Wanderley. "Análise faunística de dípteros necrófagos : ecologia e aplicação forense /." Botucatu : [s.n.], 2009. http://hdl.handle.net/11449/87814.
Анотація:
Orientador: Wesley A. C. GodoyBanca: Cláudio José Vanzuben
Banca: Marcelo Nougueira Rosa
Resumo: A Entomologia Forense é a ciência que aplica o estudo dos insetos a procedimentos legais. As pesquisas nesta área são feitas desde 1850 e nas últimas décadas vêm obtendo progressos. Esse trabalho visa constituir um banco de dados regional que possa servir como referencial científico nas investigações criminais e estimativas pós-morte, como também no estudo de casos reais que chegaram até o NPML (Núcleo de Perícias Médico-legais) Bauru, Estado de São Paulo. Análises faunísticas foram realizadas em 23 corpos, que foram conduzidos até o NPML. Os corpos eram de vítimas que sofreram morte violenta ou morte natural, para investigação da causa morte, atendendo a solicitação de autoridade competente. Na análise realizada foram encontradas duas famílias de dípteros necrófagos: Calliphoridae e Sarcophagidae. Dentre os exemplares de dípteros coletados da família Calliphoridae a espécie mais abundante foi Chrysomya albiceps (49,30%), seguida, por C. megacephala (30,56%), e Lucilia eximia (8,13%). Além destas espécies, foram também encontradas Lucilia cuprina (5,69%), Cochliomyia macellaria (1,45%) e Lucilia sericata (1,21%). Espécimes da família Sarcophagidae totalizaram 3,66% dos insetos coletados. Os vinte e três corpos analisados constituíram-se em 23 casos detalhadamente investigados, associando a presença dos insetos com as informações dos laudos necroscópicos
Abstract: Forensic entomology is the science, which applies insects for legal proceedings. Researches in this area have been performed since 1850 and in the last years researchers have obtained success. In this study a regional data set is presented to be used as a scientific reference in criminal investigations and post mortem interval estimates, as well as to be employed in real cases from the NPML (Núcleo de Perícias Médico-legais) of Bauru, São Paulo. Fauna analysis was performed in 23 corpses, which were led to the NPML. Corpses were victims, which have suffered violent or natural death, separated to investigate the death cause in response to requirements by authorities. In the analysis performed two families of necrophagous dipterans were found, Calliphoridae and Sarcophagidae. The most abundant Calliphoridae species was Chrysomya albiceps (49.30%), followed by C. megacephala (30,56%) and Lucilia eximia (8.13%). Other species were also found such as Lucilia cuprina (5.69%), Cochliomyia macellaria(1 ,45%) and Lucilia sericata (1.12%). The percentage of Sarcophagidae individuais was 3.66%. The twenty-three corpses analyzed resulted in 23 cases investigated in detail, associating the presence of insects with death data
Mestre
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Araujo, Rodrigo Thadeu de. "Aspectos médico-legais e preventivos dos casos de afogamentos na região de Ribeirão Preto." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/17/17143/tde-05122007-103925/.
Анотація:
Esta é a primeira descrição específica de casos de afogamento, em uma área nãolitorânea no Brasil, realizada na região de Ribeirão Preto, Estado de São Paulo. É uma região cujos padrões sociais são comparáveis aos de países desenvolvidos. Um total de 89 casos foi analisado. Esses casos ocorreram entre 2001 e 2004, com coeficiente de mortalidade de 2,44 casos por 100.000 habitantes por ano. Foi delineado o padrão de afogamentos fatais avaliando-se os parâmetros de idade, sexo e sazonalidade. O perfil predominantemente observado foi o de homens brancos, em idade economicamente produtiva (15 a 59 anos), acidentalmente afogados em rios e represas de áreas rurais, de ocorrência durante o verão e o outono. O estudo dessas fatalidades pode permitir o desenvolvimento de programas de prevenção de afogamento em áreas não-litorâneas e não-turísticas no Brasil e em outros lugares do mundo.This is the first specific description of drowning cases in a non-coastal area of Brazil, in the locality of Ribeirão Preto, State of São Pauloa region with standards of living comparable to those in developed countries. A total of 89 cases were analyzed. These cases occurred between 2001 and 2004, at a rate of 2.44 cases per 100,000 habitants per year. The pattern of drowning fatalities in relation to parameters of age, sex, seazonality is discussed. The predominant profile observed was that of the Caucasian male of economically productive age (15 to 59 years), accidentally drowned in rivers and dams in rural areas during the summer and autumn. Observation of the pattern of fatalities may permit the development of a program of prevention of drowning in non-coastal, non-tourist areas in Brazil and elsewhere.
18
Ward, Jennifer. "Origins and development of forensic medicine and forensic science in England, 1823-1946." Thesis, n.p, 1993. http://ethos.bl.uk/.
19
Fernandes, Clemente Maia da Silva. "Análise das reconstruções faciais forenses digitais caracterizadas utilizando padrões de medidas lineares de tecidos moles da face de brasileiros e estrangeiros." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/23/23148/tde-03072010-103917/.
Анотація:
A preocupação com a identificação, que é o processo pelo qual se determina a identidade, é bastante antiga. Atualmente, as relações sociais ou exigências civis, penais, administrativas e comerciais necessitam de sua comprovação. A identificação de pessoas mortas é fundamental, não somente para aplacar as necessidades emocionais de seus amigos e familiares, como também para que providências legais relativas ao óbito possam ser tomadas. Infelizmente, amiúde ocorrem situações em que corpos chegam aos Institutos Médico-Legais em estado de putrefação ou esqueletização, e não são identificados. Em tais situações, análises antropométricas para estimar, por exemplo, idade, gênero e estatura, são de grande valia. Nestes casos, a reconstrução facial forense será muito importante, pois pode possibilitar o reconhecimento e, por conseguinte, aumentar consideravelmente as chances de identificação. A reconstrução facial forense tridimensional pode ser manual ou digital. A reconstrução facial forense digital tornou-se possível com o advento da Tecnologia da Informação, imaginologia médica e novos softwares de imagem 3D e de reconstrução. Para a realização da reconstrução facial, são necessários dados relativos à espessura dos tecidos moles da face. Não há na literatura registros de trabalhos de reconstrução facial digital realizados com dados de tecidos moles obtidos a partir de amostras constituídas por sujeitos brasileiros. Há duas tabelas de espessura de tecidos moles publicadas para a população brasileira: uma obtida a partir de medidas realizadas em cadáveres frescos (padrão cadáveres frescos), e outra a partir de medidas em exames de ressonância magnética (padrão ressonância magnética). O objetivo do presente trabalho foi realizar três diferentes reconstruções faciais forenses digitais caracterizadas (com cabelo, cílios e sobrancelha) de um sujeito brasileiro (realizadas a partir de um padrão internacional e dois padrões nacionais de espessura de tecidos moles da face), e avaliar as reconstruções faciais forenses digitais comparando-as com fotografias do próprio indivíduo e de outros nove sujeitos. Para isso, foram utilizadas imagens DICOM de uma Tomografia Computadorizada (TC) cedidas por um voluntário que, uma vez convertidas, foram utilizadas para a efetivação das reconstruções faciais digitais. Uma vez realizadas as três reconstruções, as mesmas foram comparadas com fotografias do voluntário que teve a sua face reconstruída e outros nove sujeitos. Trinta examinadores participaram desta tentativa de reconhecimento. O sujeito-alvo, que teve a sua face reconstruída, foi reconhecido por 26,67% dos examinadores na reconstrução realizada com o Padrão nacional de Ressonância Magnética, 23,33% na reconstrução realizada com o Padrão nacional de Cadáveres Frescos e 20,00% na reconstrução realizada com o Padrão Internacional, tendo sido o sujeito mais reconhecido nos dois primeiros padrões. Os reconhecimentos acertados do sujeito-alvo indicam que a reconstrução facial forense digital, realizada com parâmetros empregados neste trabalho, pode ser ferramenta útil para, havendo um ou vários sujeitos reconhecidos, chegar-se a uma identificação positiva.The concern with the identification, that is the process by which the identity is determined, is quite old. Currently, the social relations or civil, criminal, administrative and commercial requirements need its evidence. The identification of deceased persons is essential not only to assuage the emotional needs of their friends and family, but also to allow legal actions related to death. Unfortunately, situations often occur when bodies arrive at the Medico-Legal Institutes in a state of putrefaction or skeletonization, and are not identified. In such situations, anthropometric analysis to estimate, for example, age, gender and height, are of great value. In these cases, forensic facial reconstruction is very important because it may serve to recognize and therefore increase the chances of identification. The three-dimensional forensic facial reconstruction can be manual or digital. The digital forensic facial reconstruction was made possible with the advent of Information Technology, medical imaging and new 3D image and reconstruction softwares. To perform facial reconstruction, data on the thickness of the soft tissues of the face are necessary. There is no literature records of facial reconstruction works carried out with digital data of soft tissues obtained from samples of Brazilian subjects. There are two tables of thickness of soft tissue published for the Brazilian population: one obtained from measurements performed in fresh cadavers (fresh cadavers pattern), and another from measurements on magnetic resonance imaging (magnetic resonance pattern). The aim of this study was to perform three different characterized digital forensic facial reconstructions (with hair, eyelashes and eyebrows) of a Brazilian subject (based on an international pattern and two national patterns for soft facial tissue thickness), and evaluate the digital forensic facial reconstructions comparing them to photos of the individual and other nine subjects. We used DICOM images of a computed tomography (CT) donated by a volunteer that, once converted, were used for the realization of the digital facial reconstructions. Once we\'ve performed the three reconstructions, they were compared with photographs of the volunteer who had his face reconstructed and of nine other subjects. Thirty examiners participated in this recognition attempt. The target subject, who had his face reconstructed, was recognized by 26.67% of the examiners in the reconstruction performed with the national Magnetic Resonance Pattern, 23.33% in the reconstruction performed with the national Fresh Cadavers Pattern of and 20.00 % in the reconstruction performed with the International Pattern, and the target-subject was the most recognized subject in the first two patterns. The correct recognitions of the subject indicate that the digital forensic facial reconstruction, carried out with parameters used in this study, may be a useful tool, with one or more subjects recognized to achieve a positive identification.
20
Freeman, Michael. "The role of forensic epidemiology in evidence-based forensic medical practice." Doctoral thesis, Umeå universitet, Rättsmedicin, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-81434.
Анотація:
Objectives This thesis is based on 4 papers that were all written with the same intent, which was to describe and demonstrate how epidemiologic concepts and data can serve as a basis for improved validity of probabilistic conclusions in forensic medicine (FM). Conclusions based on probability are common in FM, and the validity of probabilistic conclusions is dependant on their foundation, which is often no more than personal experience. Forensic epidemiology (FE) describes the use and application of epidemiologic methods and data to questions encountered in the practice of FM, as a means of providing an evidence-based foundation, and thus increased validity, for certain types of opinions. The 4 papers comprising this thesis describe 4 unique applications of FE that have the common goal of assessing probabilities associated with evidence gathered during the course of the investigation of traumatic injury and death. Materials and Methods Paper I used a case study of a fatal traffic crash in which the seat position of the surviving occupant was uncertain as an example for describing a probabilistic approach to the investigation of occupant position in a fatal crash. The methods involved the matching of the occupants’ injuries to the vehicular and crash evidence in order to assess the probability that the surviving occupant was either the driver or passenger of the vehicle at the time of the crash. In the second and third papers, epidemiologic data pertaining to traffic crash-related injuries from the National Automotive Sampling System-Crashworthiness Data System (NASS-CDS) was used to assess the utility and strength of evidence, such as vehicle deformation and occupant injury of a particular severity and pattern, as a means of assessing the probability of an uncertain issue of interest. The issue of interest in Paper II was the seat position of the occupant at the time of a rollover crash (similar to Paper I), and the association that was investigated was the relationship between the degree of downward roof deformation and likelihood of a serious head and neck injury in the occupant. The analysis was directed at the circumstance in which a vehicle has sustained roof deformation on one side but not the other, and only one of the occupants has sustained a serious head or neck injury. In Paper III the issue of interest was whether an occupant was using a seat belt prior to being ejected from a passenger vehicle, when there was evidence that the seat belt could have unlatched during a crash, and thus it was uncertain whether the occupant was restrained and then ejected after the seat belt unlatched, or unrestrained. Of particular interest was the relative frequency of injury to the upper extremity closest to the side window (the outboard upper extremity [OUE]), as several prior authors have postulated that during ejection when the seat belt has become unlatched the retracting seat belt would invariably cinch around the OUE and cause serious injury. In Paper IV the focus of the analysis was the predictability of the distribution of skull and cervical spine fractures associated with fatal falls as a function of the fall circumstances. Swedish autopsy data were used as the source material for this study. Results In Paper I the indifferent pre-crash probability that the survivor was the driver (0.5) was modified by the evidence to arrive at a post-test odds of 19 to 1 that he was driving. In Paper II NASS-CDS data for 960 (unweighted) occupants of rollover crashes were included in the analysis. The association between downward roof deformation and head and neck injury severity (as represented by a composite numerical value [HNISS] ranging from 1 to 75) was as follows: for each unit increase of the HNISS there were increased odds of 4% that the occupant was exposed to >8 cm of roof crush versus <8 cm; 6% for >15 cm compared to <8 cm, and 11% for >30 cm of roof crush compared to <8 cm. In Paper III NASS-CDS data for 232,931 (weighted) ejected occupants were included in the analysis, with 497 coded as seat belt failures, and 232,434 coded as unbelted. Of the 7 injury types included in the analysis, only OUE and serious head injury were found to have a significant adjusted association with seat belt failure, (OR=3.87, [95% CI 1.2, 13.0] and 3.1, [95% CI 1.0, 9.7], respectively). The results were used to construct a table of post-test probabilities that combined the derived sensitivity and (1 - specificity) rates with a range of pre-crash seat belt use rates so that the results could be used in an investigation of a suspected case of belt latch failure. In Paper IV, the circumstances of 1,008 fatal falls were grouped in 3 categories of increasing fall height; falls occurring at ground level, falls from a height of <3 meters or down stairs, and falls from ≥3 meters. Logistic regression modeling revealed significantly increased odds of skull base and lower cervical fracture in the middle (<3 m) and upper (≥3 m) fall height groups, relative to ground level falls, as follows: (lower cervical <3 m falls, OR = 2.55 [1.32, 4.92]; lower cervical ≥3 m falls, OR = 2.23 [0.98, 5.08]; skull base <3 m falls, OR = 1.82 [1.32, 2.50]; skull base ≥3 m falls, OR = 2.30 [1.55, 3.40]). Additionally, C0-C1 dislocations were strongly related to fall height, with an OR of 8.3 for the injury in a ≥3 m fall versus ground level. Conclusions In this thesis 4 applications of FE methodology were described. In all of the applications epidemiologic data resulting from prior FM investigations were analyzed in order to draw probabilistic conclusions that could be reliably applied to the circumstances of a specific investigation. It is hoped that this thesis will serve to demonstrate the utility of FE in enhancing evidence-based practice in FM.
21
Francisco, Raffaela Arrabaça. "Evolução dos casos de antropologia forense no Centro de Medicina Legal (CEMEL) da Faculdade de Medicina de Ribeirão Preto - USP de 1999 a 2010." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/17/17143/tde-06062012-134805/.
Анотація:
A Antropologia Forense é uma área de conhecimento que aplica os métodos da antropologia física e da arqueologia para coleta e análise de evidências legais, buscando estabelecer a identidade de um ser humano. O Centro de Medicina Legal (CEMEL) da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (FMRP/USP) possui um Laboratório de Antropologia Forense (LAF), criado em 2005, em um projeto de parceria com a University of Sheffield (UK) e financiado pelo British Foreign and Commonwealth Office Global Opportunities Fund. Durante esse projeto, foi elaborado um protocolo para análise de ossadas com uma estrutura científica atualizada, aplicável ao contexto social brasileiro. Durante esses doze anos ocorreu uma aparente modificação das características dos casos encaminhados ao LAF/CEMEL, o que levou à necessidade de uma análise detalhada e comparativa dos casos. A proposta deste trabalho foi de evidenciar a evolução histórica dos casos de análise antropológica forense no LAF/CEMEL. Na primeira etapa foram analisados os casos acumulados de 1999 a 2004, na segunda etapa foram analisados os casos de 2005 a 2010, totalizando doze anos; por fim, foi realizado o estudo de acordo com a causa jurídica da morte, comparando os casos encaminhados ao LAF/CEMEL com os casos totais de morte por causa externa para Ribeirão Preto e região. Com base nos relatórios de Antropologia Forense (REAFs), as características antropológicas predominantes das ossadas analisadas no LAF/CEMEL foram: indivíduos do sexo masculino, caucasianos, com uma faixa etária média de 37 anos (1999 a 2004) e 35 anos (2005 a 2010); uma média de estatura de 1,68 metros (1999 a 2004) e 1,70 metros (2005 a 2010); destreza manual não determinada (1999 a 2004) e destros (2005 a 2010); com elementos odontológicos informativos; com achados patológicos (1999 a 2004) e achados patológicos ausentes (2005 a 2010); sem roupas e/ou pertences (1999 a 2004) e com roupas e/ou pertences (2005 a 2010). Foi verificado que os parâmetros (oito) analisados para a identificação de um indivíduo através do exame antropológico aumentou de 65% (1999 a 2004) para 93% (2005 a 2010). Conclui-se que a atuação constante na análise antropológica de ossadas tem levado a uma melhor qualidade dos dados obtidos e maiores chances de identificação individual, cumprindo seu papel científico e social. Foi realizado um estudo para verificar a relação existente entre as mortes por causa externa (especificando homicídios) ocorridos nas últimas décadas em Ribeirão Preto e região e os casos de ossadas encaminhadas ao LAF/CEMEL. Os homicídios reduziram de 34 para 7 casos por 100 mil habitantes/ano. Contudo, o número médio de ossadas encaminhadas ao LAF/CEMEL se manteve em um mesmo padrão no decorrer desses doze anos, evidenciando que não há relação entre o número de homicídios ocorridos em Ribeirão Preto e região com o número de ossadas encaminhadas ao LAF/CEMEL com diagnóstico de morte por causa externa. Estima-se que os casos de ossadas encaminhadas ao LAF/CEMEL estejam ligados com maior probabilidade aos crimes de execução e ocultação de cadáver nos canaviais de Ribeirão Preto e região.Forensic Anthropology is an area of knowledge that applies the methods of physical anthropology and archeology for the collection and analysis of legal evidences, to establish the identity of a human being. The Legal Medicine Center (CEMEL), of the Faculty of Medicine of Ribeirão Preto, University of São Paulo (FMRP/USP) has a Laboratory of Forensic Anthropology (LAF), created in 2005 as a partnership project with the University of Sheffield (UK) and funded by the British Foreign and Commonwealth Office Global Opportunities Fund. During this project, a protocol for the analysis of bones with an updated scientific framework applicable to the Brazilian social context was designed. During these twelve years there was an apparent change in the characteristics of the cases referred to the LAF/CEMEL, which led to the need of a detailed analysis and comparative of the cases. The purpose of this study was to show the historical evolution of the forensic anthropological cases analysis in LAF/CEMEL. In a first step, we analyzed the cases accumulated from 1999 to 2004. In a second step, we analyzed cases from 2005 to 2010. Finally, the study was conducted in accordance to the legal cause of death comparing the cases referred to LAF/CEMEL with the total cases of death from external causes in Ribeirão Preto and surrounding region. Based on the reports of Forensic Anthropology (REAFs), the predominant characteristic of bones analyzed anthropologically in LAF/CEMEL were: males, Caucasians, with an average age of 37 years (1999-2004) and 35 years (2005 to 2010 ), an average height of 1.68 meters (1999 to 2004) and 1.70 meters (2005 to 2010), handedness is non-specific (1999-2004), right-handed (2005-2010), with elements of dental information, with pathological findings (1999-2004) and no pathological findings (2005-2010), with clothing and/or belongings (1999-2004) and clothing and/or belongings (2005-2010). It was found that the parameters (eigth) analysed to identifying an individual only through the anthropological examination increased from 65% (1999 to 2004) to 93% (2005 to 2010). We conclude that the constant action in the anthropological analysis of bones has improved the quality of data and increased the likelihood of identifying, fulfilling its scientific and social role. A study was conducted to investigate the relationship between deaths from external causes (homicides) in recent decades occurred in Ribeirão Preto and surrounding region and the bones referred to LAF/CEMEL. Homicides decreased from 34 to 7 cases per 100 000 inhabitants/year. However, the average number of skeletons sent to LAF/CEMEL remained in the same pattern during these twelve years,, showing that there is no relationship between the number of homicides occurred in Ribeirão Preto and surrounding region with the number of bones sent to LAF/CEMEL diagnosed with death from external causes. It is estimated that the cases referred to the LAF/CEMEL bones are most likely linked to the crimes of running and hiding a corpse in the sugar cane fields of Ribeirão Preto and surrounding region.
22
Lu, Andy. "Forensic analysis on wireless medical devices." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2022. https://ro.ecu.edu.au/theses/2541.
Анотація:
The number of Internet of Things (IoT) devices is forecast to grow to over 25 billion by 2030, with the healthcare IoT market projected to grow to 25.9% of IoT devices by 2028 worldwide. However, with new and growing technologies come new types of risks. Current risk assessment and risk management methods haven’t been designed to anticipate or predict these risks. IoT risks relate to openness and lack of standardisation, linking and connectivity between the devices and the lack of skilled support for IoT devices and networks. These factors put medical IoT devices and, by extension, their users at risk from cyber threats. Additionally, the attack surface for the medical IoT has not been fully mapped, nor have the risks been fully assessed. The lack of coverage means increased risk for manufacturers, medical facilities, and potentially, patients. This project evaluates the effectiveness of how new and emerging wireless and connected medical devices can be managed and analysed through a digital forensic framework. An initial analysis of the currently available frameworks showed that they did not address the nuances of implementing a wireless or connected medical device into a healthcare organisation.
Digital forensic frameworks that were deemed relevant to wireless medical devices were selected and tested against several currently available wireless medical devices. Four frameworks were tested across four devices each. The outcome was that none of the frameworks was fully able to effectively manage wireless medical devices (at least in terms of the objectives of digital forensics), with each missing elements that would aid an investigator or a hospital organisation in the case of a cyber-related incident.
These results led to the synthesis and testing of a framework that addressed the missing elements. The framework emphasises forensic readiness planning and risk management. The synthesised framework was tested against a new device. The results of the test found that the synthesised framework was effective in both the proactive digital forensics approach and reactive approach. The testing found that the framework performed better than the other tested frameworks, containing additional phases and steps that were advantageous in preparing and reacting to incidents involving wireless medical devices.
23
Nygren, Ebba, and Oona Palokangas. "Fysisk aktivitet kan leda till ökat välbefinnande hos patienter inom rättspsykiatrisk vård : En kvalitativ studie om hur personalen inom rättspsykiatrisk vård upplever användandet av fysisk aktivitet som hälsopromotiv insats." Thesis, Luleå tekniska universitet, Institutionen för hälsovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-79528.
Анотація:
I dagens samhälle finns ett stort behov av att förbättra den psykiska hälsan hos befolkningen. En liten del av personer med diagnostiserad psykisk ohälsa hamnar inom rättspsykiatrisk vård – personen har då begått ett brott under psykisk störning. Syftet med studien var att undersöka den rättspsykiatriska vårdpersonalens tankar om hur fysisk aktivitet används som hälsopromotiv insats i dagsläget, vad som kan förbättras och hur det kan främja patienternas hälsa. För att besvara syftet har kvalitativ forskning gjorts, med öppna brev som metod. De 15 personer som deltog i studien fick skriva sin berättelse utifrån meningen “Nu ska jag berätta hur vi kan använda fysisk aktivitet som hälsopromotiv insats för att förbättra hälsan hos patienterna… “. Data analyserades med hjälp av manifest innehållsanalys, som resulterade i tre underkategorier, samt en huvudkategori; “Ju högre grad patienterna har möjlighet att röra på sig, desto bättre för deras välbefinnande” med underkategorierna “Stillasittande vardag”, “Bredare utbud av aktiviteter” och “Ökat välbefinnande”. Resultatet visade att fysisk aktivitet som hälsofrämjande insats inte används i den utsträckning som det skulle kunna, som det ser ut idag, inom rättspsykiatrisk vård. Slutsatsen är att fler hälsopromotiva insatser i form av fysisk aktivitet behövs inom rättspsykiatrin, och att den troliga effekten skulle resultera både i förbättrad fysisk och psykisk hälsa hos patienterna.In today's society, there is a great need to improve the mental health of the population. A small proportion of people with diagnosed mental illness end up in forensic psychiatric inpatient care when they have committed a crime under a mental disorder. The purpose of the study was to investigate the forensic psychiatric care staff's thoughts on how physical activity is used as a health-promoting input in the current situation, what can be improved and how it can promote patients' health. To answer the question, qualitative research has been done with open letters as a research method. Fifteen forensic psychiatric care staff participated in the study, and were encouraged to write their story based on the sentence “Now I will tell you how we can use physical activity as a health promotion initiative to improve the health of the patients…”. The data was analyzed using manifest content analysis, which resulted in three subcategories as well as one main category; “The more patients are able to move, the better for their well-being” with the subcategories “Sedentary everyday life”, “Wider range of activities”, and “Increased well-being”. According to the results, health-promoting input in the forensic psychiatric care is not used in the extent it could be used. The conclusion is that more health-promoting interventions in the form of physical activity are needed in forensic psychiatric inpatient care, and it is presumed that increased physical activity would result in both improved mental health and physical health among the patients.
24
Westwood, Ellen Anne. "The use and outcomes of Dialectical Behaviour Therapy for forensic populations and non-forensic adolescent populations." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7646/.
Анотація:
Dialectical Behaviour Therapy (DBT) is a type of “talking therapy”, developed from cognitive behavioural therapy; it has been adapted to help people who experience emotions, often females diagnosed with Borderline Personality Disorder (BPD). With its growth in popularity, DBT has been adapted and used with other populations - not just adult females with BPD – to include forensic populations (i.e. people who are in contact with services due to criminal behaviour) and adolescent populations. This thesis contains a systematic literature review investigating the use of DBT in Forensic Services and an empirical study that utilised a qualitative design to explore adolescents and parents’ experiences of DBT.
25
Machado, Marcos Paulo Salles 1974. "Análise retrospectiva dos exames realizados no serviço de antropologia forense do Instituto Médico Legal Afrânio Peixoto, Rio de Janeiro-Brasil = Retrospective analysis of the skills performed in the forensic anthropology service of the Afrânio Peixoto Medical Legal Institute, Rio de Janeiro-Brasil." [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290733.
Анотація:
Orientador: Eduardo Daruge JuniorDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-27T18:33:28Z (GMT). No. of bitstreams: 1 Machado_MarcosPauloSalles_M.pdf: 1542270 bytes, checksum: 2ded2442ee636b63adfcabdc553824aa (MD5) Previous issue date: 2015
Resumo: O objetivo deste trabalho foi realizar a análise retrospectiva dos casos investigados nos dois primeiros anos de funcionamento do Serviço de Antropologia Forense (SAFO) do Rio de Janeiro. Foram recebidas 66 requisições de exames expedidas por autoridades policiais ou judiciárias e cada requisição deu origem a um laudo. Seis requisições continham ossos de origem não humana, três representavam materiais sem interesse forense, enquanto uma outra requisição solicitava exame complementar. As demais 56 requisições continham ossos de 74 diferentes indivíduos, pois nove requisições encaminhavam ossos de mais de uma pessoa. Cinco das 74 ossadas não deram entrada no laboratório do SAFO por terem sido identificados pelo Serviço de Odontologia Forense ou pelo Setor de Necropapiloscopia. Logo, 69 ossadas foram periciadas e o resultados desses exames compõe o presente estudo. Dentre as 56 requisições, 47 (83%) eram constituídas de remanescentes de um único indivíduo, enquanto 9 (17%) traziam ossos de mais de uma pessoa. Menos de 18,8% das 69 ossadas tiveram mais de 95% dos ossos do corpo recuperados, enquanto que apenas 10,14% das 47 ossadas encontradas completamente esqueletizadas tiveram mais de 50% dos seus ossos recuperados. O perfil biológico resultante dos exames das ossadas revelou que a amostra era predominantemente constituída por homens (80%), caucasoides (32%), adultos-jovens entre 21-50 anos (54%) constituindo o grupo de risco. O trauma mais frequentemente observado foi o contundente, correspondendo a 33,3% dos casos, seguido do perfurocontundente, observado em 31,4%. A região mais atingida foi a crânio-cervical, atingida em 42% dos traumas. A relação mais alta observada entre um tipo de trauma e uma região específica do corpo foi a ação perfurocontundente incidindo sobre a região crânio-cervical, correspondendo à 27,3%. 52,1% das ações perfurocontundentes foram observadas na região crânio-cervical. Por fim, a analise dos locais de encontro de ossadas evidenciou um grande número de encontro reduzido a poucas áreas da cidade, revelando que existe concentração da violência
Abstract: The aim of this study was to perform a retrospective analysis of the cases investigated in the first two years of the Forensic Anthropology Service (SAFO) of Rio de Janeiro. Over these two years the laboratory received 66 examination requisitions requested by police or judicial authorities and each of these requests has resulted in a report. Six corresponded to non-human material, other 3 represented material without forensic implications and another one corresponded to a complementary examination requisition. The remaining 56 requisitions comprised a total of 74 different individuals, once there were 9 cases with bones of more than one person. Five out of 74 skeletal remains did not enter the SAFO laboratory because they had been positively identified by the Forensic Dentistry Office or through Necropapiloscopy. Therefore 69 skeletal remains were examined and the resulting data compose the present study. Out of 56 requisitions, 47 (83%) presented skeletal remains of single individuals; whereas the other 9 (17%) conteined bones of more than one individual. Less than 18.8% of 69 skeletal remains recovered comprised more than 95% of body bones, wile only 10.14% of the 47 fully skeletonized remains had more than half of the bones recovered. The biological profile established after skeletal remains were investigated showed that the sample was made up mainly of male subjects (80%), caucasian (32%), young-adults ranging from ages 21-50 (54%), considered as the risk group. Injuries most frequently observed were blunt trauma (33.3%), followed by gunshot (31.4%). The most susceptible body regions were the head and neck, affected in 42% of cases. The highest correlation between type of trauma to a specific body part was observed with gunshot injuries to the head-neck region, corresponding to 27.3% of the cases. Gunshot trauma represented 52.1% of injuries found on the head and neck. Analysis of the places where the remains were recovered reveled a great number of cases restricted to a few areas of the city, which shows the violence is concentrated in these localities
Mestrado
Odontologia Legal e Deontologia
Mestre em Biologia Buco-Dental
26
Soares, Andjara Thiane Cury. "Perfil antropológico das ossadas analisadas no Centro de Medicina Legal (CEMEL) da Faculdade de Medicina de Ribeirão Preto - USP." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/17/17143/tde-13012009-172238/.
Анотація:
SOARES, A.T.C. Perfil antropológico das ossadas analisadas no Centro de Medicina Legal (CEMEL) da Faculdade de Medicina de Ribeirão Preto USP. 2007. 169 p. Dissertação (Mestrado) Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, 2007. A Antropologia Forense é uma área do conhecimento que aplica os métodos da antropologia física e arqueologia para coleta e análise de evidências legais, buscando estabelecer a identidade de um ser. O Centro de Medicina Legal (CEMEL) da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (FMRP-USP) possui um Laboratório de Antropologia Forense, criado em 2005 durante o desenvolvimento de um projeto em parceria com a University of Sheffield (UK) e financiado pelo British Foreign and Commonwealth Office Global Opportunities Fund. Durante esse projeto, um protocolo para análise de ossadas foi implementado dentro de uma estrutura científica atualizada para aplicação em contextos de importância social. Após dois anos de funcionamento, constatou-se que muitas das ossadas encaminhadas ao CEMEL estavam incompletas, tornando a realização de um perfil biológico difícil e postergando a sua identificação devido à ausência de informações e literatura nacional recente sobre Antropologia Forense. Assim, 42 ossadas humanas examinadas no CEMEL desde sua inauguração em 1999 foram analisadas e revisadas. Os resultados mostraram que a maioria dos casos era de indivíduos do sexo masculino, de ancestralidade caucasiana, com idade entre 32,71 e 46,29 anos, estatura entre 1,64 e 1,73m, destros, sem achados patológicos, com elementos odontológicos informativos, mas sem roupas ou outros pertences. Observou-se que 61,90% das ossadas possuíam menos de 50% dos ossos, com média de 79,64±52,40 ossos por caso. Os ossos mais encaminhados foram occipital, parietal esquerdo, temporal esquerdo, parietal direito, temporal direito, fêmur direito e vértebras torácicas de um a 12. Os ossos menos encaminhados foram hióide, piramidal esquerdo, falange distal superior um direita, falanges distais superiores dois a cinco direitas e falanges médias superiores dois a cinco esquerdas. Os dentes mais encaminhados foram o 2º molar superior direito e o 1º molar superior direito, enquanto que os dentes menos encaminhados foram o incisivo central superior direito, os incisivos centrais inferiores direito e esquerdo. Roupas e outros pertences estavam presentes em 20 casos, totalizando 66 itens. Trinta e um casos têm potencial para identificação somente através de técnicas de Antropologia Forense. Para os demais 11 casos provavelmente será necessário utilizar DNA para identificação. Diante dos resultados observados, discute-se a necessidade de melhorias na capacitação técnica e infra-estrutura, assim como a forma de coleta e o transporte, de forma a aumentar o número de elementos ósseos coletados e, conseqüentemente, de informações úteis para identificação. Dois crânios apresentaram evidência de craniotomia realizada durante necropsia e um apresentou resíduos de vela, o que sugere que podem ter sido retirados indevidamente do local de sepultamento, ou até mesmo vendidos, além da sua utilização em rituais religiosos. Conclui-se que campanhas de conscientização e melhorias na fiscalização dos cemitérios podem reduzir os casos de roubos e comércio de elementos ósseos.SOARES, A.T.C. Anthropological profile of the skeletons analyzed at the Medico Legal Centre (CEMEL) of the Faculty of Medicine of Ribeirão Preto USP. 2007. 169 p. Dissertation (Masters Degree) Faculty of Medicine of Ribeirão Preto, Ribeirão Preto, 2007. Forensic Anthropology is an area of knowledge that applies the methods of physical anthropology and archeology to the collection and analysis of legal evidence, including the search for individual identity. The Medico Legal Centre (CEMEL) of the Faculty of Medicine of Ribeirão Preto of University of São Paulo (FMRP-USP) has a Laboratory of Forensic Anthropology, created in 2005 during a development project in partnership with the University of Sheffield (UK) and financed by the British Foreign and Commonwealth Office Global Opportunities Fund. During this project, a protocol for skeletal analysis was implemented within an upgraded scientific infrastructure for application in a socially important context. After two years of work, it was noticed that many skeletons forwarded to CEMEL were incomplete, making completion of a biological profile difficult and postponing identification due to the lack of information and recent national literature about Forensic Anthropology. Therefore, 42 human skeletons examined at CEMEL since its inauguration in 1999 were analyzed and reviewed. The results showed most cases to be of male individuals, of Caucasian ethnic origin, aged between 32.71 and 46.29 y.o., of height between 1.64 and 1.73 m, right handed, without any pathological findings, with informative odontological findings, but with no clothes or other belongings. It was observed that 61.90% of the skeletons were less than 50% complete, with a mean of 79.64±52.40 bones per case. The most frequently found bones were the occipital, left parietal, left temporal, right parietal, right temporal, right femur and one to 12 thoracic vertebrae. The least frequent bones were the hyoid, left triquetral, 1st right superior distal phalanx, two to five right superior distal phalanges and two to five left superior intermediate phalanges. The most frequently found teeth were the right superior 2nd molar and right superior 1st molar, while the least frequent teeth were the right superior central incisor, and right and left inferior central incisors. Clothes or other belongings were present in 20 cases, totaling 66 items. Thirty one cases have potential to be identified only via forensic anthropological analysis. For the remaining 11 cases it will be probably necessary to use DNA for identification. In view of these observations the necessity for improvements in technical qualifications and infrastructure are discussed, as well as recovery and transport, in order to maximize the number of skeletal elements recovered andas a consequenceof information useful in identification. Two skulls presented evidence of a craniotomy conducted during autopsy and one of candle residue, suggesting that it may have been taken improperly from the place of burial, or even sold, for its use in religious rituals. It is concluded that public awareness campaigns and better inspection of cemeteries may reduce cases of robbery and trade in skeletal remains.
27
Morris, Neil Kenneth. "HIV in South African forensic medical practice." Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/31133.
Анотація:
HIV has become a focal point for South Africa and many other parts of the world. This is owing to the dire implications the disease holds for the human race. Accurate research needs to be done in order to determine the effectiveness of current prevention, treatment and funding strategies so that future strategies can be implemented with greater success. All organisations, whether governmental or non-governmental, need to be aligned in their strategies and campaigns in order to be maximally effective.
In the South African forensic setting, it is not routine to test a decedent for their HIV status unless a needle stick injury or occupational mishap occurs. This means there is very little information on the current prevalence of HIV in the other than natural death population of South Africa. In order to address the lack of information three main areas of research have been identified as the most urgent to obtain reliable information on.
The first was a study done to document the HIV prevalence in the general Pretoria MLL population for a month. All cases admitted to the Pretoria MLL were tested for their HIV status and this was documented and analysed. The second study population was the suicide population in the Pretoria MLL. It is hypothesised that the suicide populations HIV prevalence would be higher than the general population’s prevalence rate. The third study shed light on the sub population of Sudden Unexplained and Unexpected Deaths (SUU-Death) that are admitted to the Pretoria MLL. The results of all three studies determined that the prevalence of HIV in the Pretoria MLL is higher than the current statistics for South Africa.
South African forensic medical practice has the context and potential to provide large amounts of accurate information regarding the HIV pandemic in South Africa.Dissertation (MSc)--University of Pretoria, 2011.
Forensic Medicine
MSc
Unrestricted
28
Williams, Graham Andrew. "Identification and resolution of capability gaps in forensic science." Thesis, University of Huddersfield, 2012. http://eprints.hud.ac.uk/id/eprint/17500/.
Анотація:
Although forensic biology is a powerful tool in criminal investigations, there are a number of capability gaps; namely, the interpretation of low-level DNA mixtures, associating the DNA profile with a body fluid, and the issue of consent in sexual offences. A research strategy was developed that utilises whole genome amplification (WGA), messenger RNA and microRNA analysis, DNA profiling, and clothing damage analysis. An evaluation of a WGA technique – multiple displacement amplification - with and without a macromolecular crowding agent, indicated that this may be of use for DNA samples containing certain mixing ratios; however, for this to be truly of use, knowledge of the nature of the sample preanalysis is required, which is not feasible in a forensic environment. A SYBR Greenbased mRNA gene expression test was developed that was capable of distinguishing between saliva and blood by using relative quantitation on real-time PCR. However, the low specificity of the SYBR Green meant that a higher number of controls were required for this to work at forensic standard. A single channel simultaneous analytical test for DNA and microRNA was also developed, which meant that it could be possible to definitively identify the body fluid origin of a DNA profile. This represented a significant step forward in improving forensic biology capability. Reconstruction studies were carried out in response to a sexual assault case where consent was an issue. This study demonstrated that it was possible to cause significant damage to a bra without causing damage to the hook and eye fastening; thus, negating a hypothesis offered by the defence. A long term research strategy has been developed and significant progress has been made in improving the capability of the operational forensic biologist.
29
Mullen, Carrie. "Quality Assurance of forensic investigations in toxicology and traffic safety." Thesis, University of Glasgow, 2014. http://theses.gla.ac.uk/5465/.
Анотація:
The work described in this thesis deals with three aspects of quality assurance in the field of forensic toxicology: proficiency testing schemes, validation of analytical methods for the piperazine group of abused drugs and validation of the police field impairment test, used at the roadside to test drivers for drug-induced impairment. Proficiency Testing: Long term reviews were performed for two forensic external quality assurance schemes. Rounds 30 (in 2007) to 48 (in 2012) of the UKAS-accredited commercial Quartz Forensic Blood Toxicology Proficiency Testing Scheme (PTS), and a ten year period from 1999 to 2009 of the freely-available United Nations Office on Drugs and Crime (UNODC) International Collaborative Exercises (ICE). Only limited ICE data could be made available as much of the original data had been stored on a database which had become obsolete, hence the data were only available as the original results forms provided to UNODC by the ICE participants. Data was entered to Microsoft Excel® spreadsheets and Microsoft Access® databases from the original forms for the years 1999, 2001 (2 rounds), 2003 (2 rounds) and 2005 (2 rounds), and summary data was extracted from the UNODC round reports for the years 2007, 2008 and 2009. Four methods of scoring quantitative performance were reviewed and the most suitable, a z-score using an assigned ‘true’ value and a percentage of the true value as acceptable deviation, was applied to reanalyse the participants’ results and assess their performance. Methods of scoring proficiency which relied upon participants’ data to determine acceptable variation were found merely to describe the data rather than challenge participants on whether or not they were performing fit-for-purpose analyses. Factors such as participation, analytes tested, participants’ methods of analysis and participants performance were summarised for each scheme before the performance of the two schemes, and that of their participants, were compared. ICE tested more analytes per annum but from a smaller test menu than Quartz. This resulted in more repetitive testing and allowed for some trend analysis and performance monitoring. It was not possible to observe performance trends with Quartz due to the wide variety of analytes tested. The smaller array of potential analytes and more repetitive nature of ICE testing also meant that performance monitoring and detection of bias were easier to perform, and ICE was shown to be more effective as external quality assurance (EQA). Quartz provided a good educational resource as it incorporated the wide range of drugs which a forensic toxicology laboratory could realistically encounter. Following the review, however, it was recommended for QUARTZ that, to provide a safeguard against bias, more repetitive testing was required and this has now been adopted. Piperazines: All piperazine analogues are now illegal in the UK, registered as Class C of the Misuse of Drugs Act (1971) and schedule 2, part III of the Misuse of Drugs Regulations (2001). Piperazines can elicit similar effects to some ATS and methods for their detection should be available in forensic toxicology laboratories. In the present study, methods were developed for the detection of a range of piperazines in blood using LC-MS/MS (p-MeOPP, p-FPP, BZP, o-MeOPP, p-MPP and TFMPP) and GC-MS (p-FPP, BZP, TFMPP, p-MPP, o-MeOPP, m-CPP, p-MeOPP and p-CPP). Quality assurance required both methods to be validated. For all piperazine analytes accuracy was within ±15% (20% at low concentrations) and precision was within 15% (20% at low concentrations). For both methods LLOD of all analytes was 5 ng/ml of blood and upper limit of quantification was 2 µg/ml of blood. For the GC-MS method lower limits of quantification (LLOQs) were in the range 20 to 30 ng/ml of blood. For LC-MS/MS, LLOQs ranged from 50 to 60 ng/ml of blood, although quantification by the LC-MS/MS method was restricted by the lack of availability of appropriate internal standards. There were no apparent significant matrix effects and recovery by both methods was >60 % and, therefore, acceptable. Short term stability of the piperazine analytes was investigated. Piperazines remain sufficiently stable when stored in the fridge for at least one week, and are stable through three freeze-thaw cycles. There was no detectable degradation when blood samples were left on the bench-top or when extracted ‘in-process’ samples were left in the autosampler for up to 72 hours. The LC-MS/MS method could provide a readily applicable screening method. A small aliquot of a basic drug extract could be screened by LC-MS/MS for the presence of piperazines, leaving the majority of the extract for other analyses, for example, piperazines confirmation or amphetamines analysis. The GC-MS method was suitably validated to provide quantification but application to casework samples remains to be evaluated. It is recommended that piperazine testing be performed for all suspected MDMA or ‘club drug’ intoxication cases. The Field Impairment Test: The detection of drugged drivers primarily depends on the current method which is the driver field impairment test (FIT). FIT comprises measurement of pupil diameter and four physical tasks (the Romberg balance test, walk and turn test, one legged stand and finger to nose test) intended to simultaneously test comprehension, short term memory, balance and motor function. Despite FIT having ISO accreditation, it has been recognised that police officers lack confidence with the protocol and do not apply the test as often as is necessary. The main difficulty arises from the requirement to make a subjective judgement of impairment and officers lack confidence in their ability to do so. FIT has never been fully validated. The present study was designed to meet the urgent requirement to develop FIT into an objective measurement, by determining what constitutes “normal” performance in FIT by unimpaired adults of different ages. FIT performance was recorded for 79 individuals, a statistically determined cohort size, confirmed by breath and oral fluid analysis not to be under the influence of impairing substances. Each error made during FIT, as defined by the FIT standard operating procedure, was recorded and collated in a Microsoft Excel® spreadsheet for analysis. It was found that the definition of ‘errors’ was too stringent as many which are required to be recorded are normal physiological or behavioural characteristics, such as body sway, and most subjects would be unable to complete the task without displaying them. A less stringent, evidence-based definition of “error” was developed which allowed statistically more significant analysis to be performed on the FIT results. A statistically significant difference (P=0.00578) was shown to exist between the FIT performance of individuals under the age of forty years and those aged forty and over. Based on the principles of a PTS, robust mean and standard deviation were used to determine what constituted acceptable performance. Those in the younger age group could be considered impaired if the police officer witnessed more than seven errors, or, in the older age group, more than fifteen errors. Using these criteria the frequency of false positives, i.e. unimpaired drivers being assessed as impaired is estimated to be (less than 3%). Also, the ranges of errors observed in both groups was large and overlapped, such that it may be possible for an impaired person to appear unimpaired. This requires further investigation.
30
Sturgess, Danielle. "Engagement in treatment amongst a forensic population." Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/6559/.
Анотація:
This thesis aims to provide a detailed understanding of treatment engagement amongst forensic populations. Following an introduction outlining the current theoretical thinking in the area, Chapter 2 presents a systematic literature review exploring reasons for completion/non-completion of treatment from an offender's perspective. Consensus regarding reasons for treatment completion/non-completion was found. Reasons provided supported the majority of factors outlined in the Multifactor Offender Readiness Model (MORM), a model of treatment readiness. Research in this area was limited; no papers exploring adolescents' perspectives were identified. Implications for practice are discussed and areas for future research highlighted. Furthering existing research, Chapter 3 presents a mixed methods research study exploring the reasons why young people, detained in a secure hospital setting choose to attend/not attend sessions. Using thematic analysis several themes were identified. Factors relating to the young person, treatment and the organisation were identified, supporting the MORM. Chapter 4 presents a critical review of the Corrections Victoria Treatment Readiness Questionnaire (CVTRQ), a measure of treatment readiness developed using the internal factors of the MORM. This chapter explores the overall development and psychometric properties of the CVTRQ, highlighting its strengths and limitations. An overall discussion of the work presented is provided in Chapter 5.
31
Takaichi, Kenichi. "Nail analysis in forensic toxicology for the detection of drug misuse." Thesis, University of Glasgow, 2004. http://theses.gla.ac.uk/3533/.
Анотація:
This thesis examines the use of nail as an alternative biological specimen in forensic toxicology. Nail is a difficult analytical matrix from which to extract drugs because of its tough physical composition, based on keratin. The initial part of the project investigated the use of a cryogenic grinding method for fingernail clippings. Grinding at liquid nitrogen temperatures was found to be an effective procedure and the conditions were optimised to a two or three cycle programme of freezing and grinding. Small particle sizes were obtained of approximate size 1µm. It was established that drugs could be extracted directly from nail powder with a range of solvents without the need for alkaline hydrolysis. Methanol was found to be the most effective extraction solvent, which also gave the lowest number of co-extracted interfering compounds. This procedure was subsequently used with nail samples from different types of forensic cases, including cannabis, heroin and steroid abusers. Cryogenic grinding of nail was evaluated as an extraction method for cannabinoids in nail clippings from chronic cannabis smokers. This method was also compared to the alkaline hydrolysis method. Fingernail clippings were collected with prior informed consent from volunteers attending the Edinburgh Drug Addiction Study (EDAS) clinic. The collected nail clippings were decontaminated and divided into two groups: the first group was extracted with methanol after pulverisation in a liquid nitrogen cryogenic mill; the second was extracted with ethyl acetate after hydrolysis in sodium hydroxide. In both groups deuterated cannabinoids were used as internal standards. Both sets of extracts were derivatised with BSTFA before being analysed by gas chromatography – mass spectrometry (GC-MS). Cannabidiol, ?9-tetrahydrocannabinol and 11-hydroxy-?9-tetrahydrocannibinol were quantified in both sets of extracts. 11-nor-?9 –tetrahydrocannabinol-9-carboxylic acid was only identified and quantified in the extracts resulting from the cryogenic grinding method. Cannabinoid concentrations were very low, in the range 0-4 ng/mg. These results strongly support the use of nail as a biological specimen for the detection and quantification of past exposure to cannabis, and secondly, they indicate that grinding with a cryogenic mill is a useful procedure, which yields simultaneous results for the primary psychoactive cannabinoid and its metabolites. Cryogenic grinding was then evaluated for the extraction of opioids in nail in comparison with the conventional alkaline hydrolysis method. Finger and toe nails were collected from donors with informed consent.
32
Ariffin, Marinah Mohd. "The application of novel extraction and analytical techniques in forensic toxicology." Thesis, University of Glasgow, 2006. http://theses.gla.ac.uk/4144/.
Анотація:
The aim of this study was to investigate new methods of analysis which might be applied to forensic toxicology problems including those resulting from pesticides, particularly the quaternary ammonium herbicide group, and from drugs, particularly the benzodiazepine group. In the first part of this study, an efficient method for the determination of quaternary ammonium (QA) compounds (pesticides and drugs) in human whole blood was developed. The second part of this study concerned the development of a novel sorbents for solid phase extraction using molecularly imprinted polymers (MIPs). The approach adopted was initially to synthesise a known MIP using diazepam as template then to prepare novel MIPs using other benzodiazepines and analogues of QA compounds as templates. In the first of these stages, an anti-diazepam MIP was synthesized using methacrylate acid (MAA) as the monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-liner and was then ground and prepared for use as an SPE sorbent by packing it into SPE cartridges. These cartridges were used to clean up extracts of diazepam and other benzodiazepine drugs made from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. The MISPE method was also found to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs in addition to diazepam itself. The application of the extraction method to post-mortem hair samples yielded results that were in good agreement with ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC-MS-MS method. The MISPE procedure was also compared with a conventional SPE method for analysis of benzodiazepines in hair samples. The results from MISPE protocol showed better selectivity, specificity and accuracy toward diazepam (template molecule) and other benzodiazepines that display a similar resemblance to diazepam in terms of molecular structure. The MISPE procedure was found to be simpler and to offer cleaner extracts compared to a conventional SPE method.
33
Filho, Renato Evando Moreira. "Micologia forense: a dinÃmica da microbiota fÃngica na investigaÃÃo do perÃodo post mortem." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2981.
Анотація:
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel SuperiorWith developing of forensic observations, certain species of insects and microorganisms were described as indicators of periods of the degradation of the body. However, the literature is still scarce in the field of Forensic Mycology. It was investigated the microbiological characteristics of fungi presence in the post mortem change, as well as, it esteemed the value of the mycology exams in the study. 400 collections was accomplished in 60 human bodies (34 of the bloated stage, 06 of the putrefaction stage and 20 of the skeletonization stage) at the Fortaleza city morgue and public cemeteries in the state of CearÃ, The picked material was analyzed through macro/microcharacteristics and specific biochemical tests. Ally to such analyses, was also accomplished the test of the perforation of the hair in vitro in hair of corpses in the bloated stage and in hair of healthy adults. The material gathered was analyzed at the Specialized Medical Mycology Center of the Federal University of CearÃ. With the anthropological results, it was observed that male, in the interval of the 31 to the 40 years, was more commonly attacked, being the capital (Fortaleza) the more involved in the number of deaths. In the bloated stage, among the identified filamentous fungi, the presence of four orders was observed: Order Eurotiales (63 isolated Aspergillus spp and 21 isolated Penicillium spp), Order Mucorales (4 Mucor spp), Order Hypocreales (2 Acremonium spp, 1 Trichoderma spp and 1 Fusarium spp) and Order Saccharomycetales (2 Geotrichum spp). In the yeast distribution, it was observed the orders:Saccharomycetales (44 Candida spp) and Tremellales (5 Trichosporon spp). In the putrefaction stage, it was isolated the following orders: Eurotiales (Penicillium spp 2 and Aspergillus 2) and Hypocreales 1 (Acremonium spp). Regarding the yeasts, it was just isolated the Order Saccharomycetales (Candida spp 3). In the eskeletonization stage, the following orders were observed: Order Eurotiales (Aspergillus spp 22 and Penicillium spp 18), Order Mucorales (Mucor spp 10) and Order Hypocreales (Acremonium spp 2 and Trichoderma spp 1). Regarding the yeasts, two orders were found: Tremellales (Trichosporon spp 1) and Saccharomycetales (Candida spp 1). In the hair perforation test in vitro, it was positive in the bloated stage, differing of healthy adults,where the test was negative. For conclusion, Forensic Micology is still a rich field in data and fungi can come to be a tool in the aid of human post mortem diagnosis.
Com o evoluir das observaÃÃes mÃdico-legais, determinadas espÃcies de insetos e microrganismos foram descritos como indicadores de perÃodos da degradaÃÃo do corpo. Entretanto, a literatura cientÃfica ainda à escassa no campo da Micologia Forense. Assim, investigaram-se as caracterÃsticas microbiolÃgicas dos fungos partÃcipes na mudanÃa de flora post mortem em humanos, bem como estimou-se o valor da realizaÃÃo de exames micolÃgicos no estudo cronotanatolÃgico. Foram realizadas um total de 400 coletas em 60 corpos humanos (34 do perÃodo gasoso, 06 do coliquativo e 20 do esqueletizado) examinados no Instituto MÃdico-Legal de Fortaleza e em cemitÃrios do Estado do CearÃ. Foram realizados estudos macro/micromorfÃlogicos e testes bioquÃmicos especÃficos para cada grupamento fÃngico isolado. Aliado a tais anÃlises, foi tambÃm realizado o teste da perfuraÃÃo do pÃlo in vitro em pÃlos de cadÃveres no perÃodo gasoso e em pÃlos de adultos hÃgidos. Estas identificaÃÃes foram realizadas no Centro Especializado em Micologia MÃdica da Universidade Federal do CearÃ. Com os resultados antropolÃgicos, foi observado que o sexo masculino, dos 31 aos 40 anos, foi o mais comumente acometido, sendo a capital (Fortaleza) a mais envolvida no nÃmero de mortes. No perÃodo gasoso, dentre os fungos filamentosos identificados, foi observada a presenÃa de quatro ordens: Eurotiales (63 isolados de Aspergillus spp e 21 de Penicillium spp), Mucorales (4 Mucor spp) e Hypocreales (2 Acremonium spp, 1 Trichoderma spp e 1 Fusarium spp) e Saccharomycetales (2 Geotrichum spp). No referente Ãs leveduras, verificaram-se as ordens: Saccharomycetales (Candida spp 44) e Tremellales (Trichosporon spp 5). No perÃodo coliquativo, registraram-se as ordens: Eurotiales (Penicillium spp 2 e Aspergillus 2) e Hypocreales 1 (Acremonium spp). No referente Ãs leveduras, isolou-se apenas a Ordem Saccharomycetales (Candida spp 3). No perÃodo de esqueletizaÃÃo, verificaram-se as seguintes ordens: Eurotiales (Aspergillus spp 22 e Penicillium spp 18), Mucorales (Mucor spp 10) e Hypocreales (Acremonium spp 2 e Trichoderma spp 1). No referente Ãs leveduras, registraram-se duas ordens: Tremellales (Trichosporon spp 1) e Saccharomycetales (Candida spp 1). Quanto ao teste de perfuraÃÃo do pÃlo in vitro, a positividade foi observada no perÃodo gasoso, diferindo de adultos hÃgidos, em que foi negativa. Conclui-se que o estudo da Micologia Forense ainda à um campo rico em dados e que os fungos poderÃo vir a ser uma ferramenta complementar no estudo do tempo de morte em Medicina Legal.
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Yokchue, Tanasiri. "In vitro studies of drug transformations : application to forensic toxicology." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7490/.
Анотація:
The forensic toxicologist faces challenges in the detection of drugs and poisons in biological samples due to transformations which occur both during life and after death. For example, changes can result from drug metabolism during life or from the use of formalin solution for post mortem embalming purposes. The former requires the identification of drug metabolites and the latter the identification of chemical reaction products in order to know which substances had been administered. The work described in this thesis was aimed at providing ways of tackling these challenges and was divided into two parts. Part 1 investigated the use of in vitro drug metabolism by human liver microsomes (HLM) to obtain information on drug metabolites and Part 2 investigated the chemical reactions of drugs and a carbamate pesticide with formalin solution and formalin-blood. The initial aim of part I was to develop an in vitro metabolism method using HLM, based on a literature review of previous studies of this type. MDMA was chosen as a model compound to develop the HLM method because its metabolism was known and standards of its metabolites were commercially available. In addition, a sensitive and selective method was developed for the identification and quantitation of hydrophilic phase I drug metabolites using LC/MS/MS with a conventional reverse-phase (C18) column. In order to obtain suitable retention factors for polar drug metabolites on this column, acetyl derivatives were evaluated for converting the metabolites to more lipophilic compounds and an optimal separation system was developed. Acetate derivatives were found to be stable in the HPLC mobile phase and to provide good chromatographic separation of the target analytes. In vitro metabolism of MDMA and, subsequently, of other drugs involved incubation of 4 µg drug substance in pH 7.4 buffer with an NADPH generating system (NGS) at 37oC for 90 min with addition of more NGS after 30 min. The reaction was stopped at 90 min by the addition of acetonitrile before extraction of the metabolites. Acetate derivatives of MDMA metabolites were identified by LC/MS/MS using multiple reaction monitoring (MRM). Three phase I metabolites (both major and minor metabolites) of MDMA were detected in HLM samples. 3,4-dihydroxy-methamphetamine and 4-hydroxy-3-methoxymethamphetamine were found to be major metabolites of MDMA whereas 3,4-methylenedioxyamphetamine was found to be a minor metabolite. Subsequently, ten MDMA positive urines were analysed to compare the metabolite patterns with those produced by HLM. An LC/MS method for MDMA and its metabolites in urine samples was developed and validated. The method demonstrated good linearity, accuracy and precision and insignificant matrix effects, with limits of quantitation of 0.025 µg/ml. Moreover, derivatives of MDMA and its metabolites were quantified in all 10 positive human urine samples. The urine metabolite pattern was found to be similar to that from HLM. The second aim of Part 1 was to use the HLM system to study the metabolism of some new psychoactive substances, whose misuse worldwide has necessitated the development of analytical methods for these drugs in biological specimens. Methylone and butylone were selected as representative cathinones and para-methoxyamphetamine (PMA) was chosen as a representative ring-substituted amphetamine, because of the involvement of these drugs in recent drug-related deaths, because of a relative lack of information on their metabolism, and because reference standards of their metabolites were not commercially available. An LC/MS/MS method for the analysis of methylone, butylone, PMA and their metabolites was developed. Three phase I metabolites of methylone and butylone were detected in HLM samples. Ketone reduction to β-OH metabolites and demethylenation to dihydroxy-metabolites were found to be major phase I metabolic pathways of butylone and methylone whereas N-demethylation to nor-methylone and nor-butylone were found to be minor pathways. Also, demethylation to para-hydroxyamphetamine was found to be a major phase I metabolic pathway of PMA whereas β-hydroxylation to β-OH-PMA was found to be a minor pathway. Formaldehyde is used for embalming, to reduce decomposition and preserve cadavers, especially in tropical countries such as Thailand. Drugs present in the body can be exposed to formaldehyde resulting in decreasing concentrations of the original compounds and production of new substances. The aim of part II of the study was to evaluate the in vitro reactions of formaldehyde with selected drug groups including amphetamines (amphetamine, methamphetamine and MDMA), benzodiazepines (alprazolam and diazepam), opiates (morphine, hydromorphone, codeine and hydrocodone) and with a carbamate insecticide (carbosulfan). The study would identify degradation products to serve as markers for the parent compounds when these were no longer detectable. Drugs standards were spiked in 10% formalin solution and 10% formalin blood. Water and whole blood without formalin were used for controls. Samples were analysed by LC/MS/MS at different times from the start, over periods of up to 30 days. Amphetamine, methamphetamine and MDMA were found to rapidly convert to methamphetamine, DMA and MDDMA respectively, in both formalin solution and formalin blood, confirming the Eschweiler-Clarke reaction between amine-containing compounds and formaldehyde. Alprazolam was found to be unstable whereas diazepam was found to be stable in both formalin solution and water. Both were found to hydrolyse in formalin solution and to give open-ring alprazolam and open-ring diazepam. Other alprazolam conversion products attached to paraformaldehyde were detected in both formalin solution and formalin blood. Morphine and codeine were found to be more stable than hydromorphone and hydrocodone in formalin solution. Conversion products of hydromorphone and hydrocodone attached to paraformaldehyde were tentatively identified in formalin solution. Moreover, hydrocodone and hydromorphone rapidly decreased within 24 h in formalin blood and could not be detected after 7 days. Carbosulfan was found to be unstable in formalin solution and was rapidly hydrolysed within 24 h, whereas in water it was stable up to 48 h. Carbofuran was the major degradation product, plus smaller amounts of other products, 3-ketocarbofuran and 3-hydrocarbofuran. By contrast, carbosulfan slowly hydrolysed in formalin-blood and was still detected after 15 days. It was concluded that HLM provide a useful tool for human drug metabolism studies when ethical considerations preclude their controlled administration to humans. The use of chemical derivatisation for hydrophilic compounds such as polar drug metabolites for analysis by LC/MS/MS with a conventional C18 column is effective and inexpensive, and suitable for routine use in the identification and quantitation of drugs and their metabolites. The detection of parent drugs and their metabolites or conversion and decomposition products is potentially very useful for the interpretation of cases in forensic toxicology, especially when the original compounds cannot be observed.
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Lemos, Nikolaos P. "Analysis of bile and nail as alternative biological specimens in forensic toxicology." Thesis, University of Glasgow, 1999. http://theses.gla.ac.uk/4116/.
Анотація:
This thesis examines the use of bile and nail as alternative biological specimens in forensic toxicology. In chapter 1, a brief overview of the mechanisms of substance passage into and out of membranes and fluids and matrices was presented followed by a review of the use of alternative biological specimens for analytical purposes. The experimental part of this thesis begins in chapter 2 where a simple and rapid method for the detection and quantification of dextropropoxyphene in bile and its major metabolite, norpropoxyphene, is presented. Following this, nail is examined as an alternative biological specimen in cases of medico-legal interest. Firstly, two major cannabinoids, 9-tetrahydrocannabinol and 11-nor-9-tetrahydrocannabinol-9-carboxylic acid were identified in the nail (chapter 3) by means of RIA and GC-MS. Cannabinoids were found to be present in all six cases that were analysed for cannabinoids by RIA and their concentration ranged from 0.23 ng/mg to 2.80 ng/mg (average: 1.03 ng/mg). Using GC-MS, 9-tetrahydrocannabinol was detected in 11 out of the 14 nail clipping hydrolysates after basic extraction with concentrations ranging from 0.13 ng/mg to 6.97 ng/mg (average: 1.44 ng/mg). 11-nor-9-tetrahydrocannabinol-9-carboxylic acid was not detected in any of these nail clipping hydrolysates but was detected in two of the three fingernail clipping hydrolysates extracted under acidic pH with concentrations ranging from 9.82 ng/mg to 29.7 ng/mg (average: 19.8 ng/mg). In chapter 4, the detection and quantification of diazepam, the most commonly encountered benzodiazepine, were described. Using ECD-GC, diazepam was detected in hydrolysates of all 6 sets of nail clippings in concentrations ranging from 4.37 to 87.8 ng/mg (average: 25.7 ng/mg). In chapter 5, an analytical protocol for the detection and quantification of morphine in nail clippings of known heroin abusers was presented. Finally, a protocol for the detection and quantification of methadone in nail clippings of patients attending a methadone maintenance program was presented.
36
Kan, Man-yee Elsie. "Application of neisseria gonorrhoeae molecular typing techniques in forensic medicine." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971854.
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Kan, Man-yee Elsie, and 簡文意. "Application of neisseria gonorrhoeae molecular typing techniques in forensic medicine." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971854.
38
Lerer, Leonard Brian. "Forensic epidemiology : the interface between forensic science and public health." Master's thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/25827.
39
Kuhnen, Barbara. "Mensuração da espessura de tecidos moles faciais de crianças e adolescentes brasileiros /." Araraquara, 2018. http://hdl.handle.net/11449/154033.
Анотація:
Orientador: Monica da Costa SerraResumo: Na sociedade, a identificação humana é indispensável tanto por razões legais como humanitárias. Porém, existem situações em que corpos encontrados não são passíveis de reconhecimento ou quando não há suspeição, impossibilitando a identificação. Nesses casos, análises antropológicas são de extrema importância, pois permitem estabelecer o perfil biológico do sujeito. Assim, realiza-se a Reconstrução Facial Forense (RFF) para possibilitar o reconhecimento e levar a uma possível identificação. A RFF, para ser realizada, precisa dos valores das espessuras de tecidos moles faciais (ETMF) em diversos pontos craniométricos. Essas medidas servem como guia para se estabelecer um limiar do contorno da face e podem ser influenciadas por diferentes fatores como sexo, ancestralidade, idade e índice de massa corporal (IMC). Não há na literatura tabelas de ETMFs de crianças e adolescentes brasileiros, para auxiliarem na RFF. O objetivo desse trabalho foi realizar a mensuração da espessura de tecidos moles de crianças e adolescentes brasileiros, a partir de dados de imagens de Tomografias Computadorizadas de Feixe Cônico (TCFC) de indivíduos vivos, considerando-se as variáveis sexo, idade, cor da pele e índice de massa corporal. Neste estudo, foram mensurados os 21 pontos craniométricos pré- determinados por meio do software Osírix Lite, de imagens de TCFC da região de cabeça e pescoço de 103 não adultos brasileiros (39 crianças e 64 adolescentes), obtidas junto aos arquivos de imagens de uma... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: In society, human identification is indispensable both for legal and humanitarian reasons. However, there are situations in which bodies found are not recognizable or when there is no suspicion, making identification impossible. In these cases, anthropological analyzes are extremely important, since they allow to establish the biological profile of the subject. Thus, Forensic Facial Reconstruction (FFR) is performed to enable recognition and lead to a possible identification. The RFF, to be performed, needs the values of facial soft tissue thicknesses (FSTT) at several craniometric points. These measures serve as a guide to establish a threshold of the face contour and can be influenced by different factors such as sex, ancestry, age and body mass index (BMI). There are no tables in the literature of FSTTs of Brazilian children and adolescents, to assist in FFR. The objective of this study was to measure the thickness of soft tissues of Brazilian children and adolescents, based on data from Cone-Beam Computed Tomography (CBCT) images of living individuals, considering the variables gender, age, skin color and body mass index. In this study, 21 pre-determined craniometric points were measured using the Osírix Lite software, from head and neck CBCT images of 103 non-Brazilian adults (39 children and 64 adolescents), obtained from the image files of a Clinic of Radiology and Dental Documentation located in the city of Araraquara - SP. According to the obtained results, there wer... (Complete abstract click electronic access below)
Mestre
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Franco, Catarina Peixoto de Pinho de Gouveia. "Marcas de mordida e a medicina dentária forense." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4600.
Анотація:
Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina DentáriaA Medicina Dentária Forense é uma ciência que estuda as aplicações dentárias em processos judiciais e que tem como objetivo a pesquisa de fenómenos psíquicos, físicos, químicos e biológicos que podem atingir ou ter atingido o Homem vivo, morto ou esqueletizado, e mesmo fragmentos ou vestígios, resultando de lesões parciais ou totais, reversíveis ou irreversíveis. O reconhecimento da sua importância está relacionado à actuação decisiva, em alguns episódios, quando há necessidade de identificação de corpos por meio do exame dentário ou identificação criminal. As marcas de mordida são geralmente vistas em casos de agressão sexual, assassinato e abuso de menores e pode ser um factor importante para levar a uma condenação. Um dos mais intrigantes, complexos e controversos desafios em Medicina Dentária Forense é o reconhecimento, registo e a análise de marcas de mordida em pele humana devido à constituição desta, dos tecidos adjacentes e movimentação do agressor e/ou vítima. O estudo das marcas de mordida é feito segundo diversas análises sendo a mais utilizada a análise comparativa. As marcas de mordida podem ser encontradas em qualquer parte do corpo, particularmente no tecido macio e carnudo como o estômago ou nas nádegas. Além disso, as marcas de mordida podem ser encontradas em objectos presentes na cena de um crime. Na pesquisa bibliográfica recorreu-se aos motores de busca PubMed, Science Direct, B-On, Elvesier, SciELO e Wiley. Utilizando as seguintes palavras-chave: “Bite Marks”, “Forensic science”. Sendo incluidos 96 artigos na elaboração desta tese além de alguns sites e livros consultados. Os objectivos deste trabalho são identificar características das marcas de mordida que permitam ao investigador concluir o causador da marca, evidenciar a sua importância no contexto da Medicina Dentária Forense (crimes sexuais, abusos infantis, agressões, etc), referenciar diversas técnicas de análise e as suas possíveis vantagens/desvantagens em cada caso. Devido à crescente violência, os crimes tornam-se mais sofisticados e aprimorados, sendo necessárias técnicas científicas desenvolvidas na investigação pericial. Dada a importância da Medicina Dentária Forense o Médico Dentista é crucial na identificação, localização, registo, denuncia e sinalização devendo haver mais formação incentivada pelas Instituições do Ensino Superior, organizações e mesmo clínicas nesta área tão essencial para prevenir a escassez dos especialistas evitando, deste modo, a falha de identificação destes episódios deteriorantes. Forensic Dental Medicine is a science of dental applications in legal proceedings and which aims to research psychic, physical, chemical and biological phenomena that can reach or have reached the living, dead or in skeletal man, and even fragments or traces resulting in partial or total, reversible or irreversible lesions. The recognition of its importance is related to the decisive role in some episodes, when there is need to identify bodies through dental examination or criminal identification. The bite marks are usually seen in cases of sexual assault, murder and child abuse and can be an important factor to lead to a conviction. One of the most intriguing, complex and controversial challenges in Forensic Dentistry is the recognition, recording and analysis of bite marks on human skin due to the establishment within the adjacent tissues and movement of the perpetrator and / or victim. The study of bite marks is done according to various analyzes and the most frequent benchmarking. The bite marks may be found anywhere in the body, particularly in soft and fleshy tissue such as the stomach or buttocks. Moreover, the bite marks can be found in objects on the scene of a crime. In the literature we used the search engines PubMed, Science Direct, B-On, Elvesier, SciELO and Wiley. Using the following words-key: “Bite Marks”, “Forensic Science”. 96 being included articles in the preparation of this thesis as well as some websites visited. The objectives of this study are to identify characteristics of bite marks that allow the researcher to conclude the cause of the brand, highlighting its importance in the context of Forensic Dentistry (sex crimes, child abuse, assault, etc.), cite several analysis techniques and its possible advantages / disadvantages in each case. Due to the increasing violence, the crimes become more sophisticated and refined, necessary scientific techniques developed in the forensic investigation being. Given the importance of the Forensic Dentistry Dentist is crucial in identifying, locating, recording, and betrays signs should be more training encouraged by Higher Education Institutions, organizations and even clinics in this area as essential to prevent the shortage of specialists avoiding this Similarly, failure to identify these episodes spoilage.
41
Savka, I. H., B. Mykhailichenko, M. Stasyuk, and A. Santos. "A modern methods of investigation a fracture plane in forensic medicine." Thesis, Mat. of the 19-th IAFS world meeting, 9-th WPMO triennial meeting, 2011. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/2627.
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MARTINS, Geraldo. "Calliphoridae (Insecta, Diptera) associados às fases de decomposição de carcaças de suínos (Sus scrofa Linnaeus, 1758) em área de caatinga no estado da Paraíba." Universidade Federal Rural de Pernambuco, 2013. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5666.
Анотація:
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The objective of this study was to identify and analyze the abundance of dipteran family Calliphoridae present along the decomposing carcass of pigs in the Cariri, Paraiba State, Brazil, in two periods: rainy and dry. The study was conducted in Reserva Particular do Patrimônio Natural (RPPN) Fazenda Almas, in São José dos Cordeiros-PB. We used two pig carcasses (Sus scrofa) by period, protected from scavengers by a metal cage inside trap like "Shannon". Samples were collected daily, twice a day, for eleven days in both periods. The meteorological data of precipitation were obtained at the Centro de Previsão e Estudos Climáticos do INMET and temperature and relative humidity were obtained in situ using a digital hygrometer. The insects collected were placed in the laboratory of entomology of UFPB and identified as well. We collected 8.811 individuals of the family Calliphoridae, belonging to six species (Chrysomya albiceps (Wiedemann, 1819), Cochliomyia macellaria (Fabricius, 1775), Chrysomya putoria (Wiedemann, 1818), Chloroprocta idioidea (Robineau-Devoidy, 1830), Chrysomya megacephala (Fabricius, 1794) e Lucilia eximia (Wiedemann, 1818)), which were presented the in both the periods, although a relative abundance of species was higher in the rainy period (4.706). We measured the abundance of species per period, where C. albiceps was the most abundant in the dry period (3.244/36, 81%) and C. idioidea in the rainy period (2.860/32, 45%). There were five stages of decomposition: fresh, chromatic, emphysematous, coliquative and skeletonization stage. The emphysematous was the stage where we most collected Calliphoridae (3.923/44, 52%), followed by skeletonization (3.458/39, 25%), chromatic (726 / 8.24%), coliquative (627/7, 12%) and fresh (77/0, 87%). It was also found that the highest abundance of C. idioidea occurred on the highest elevation of relative humidity and rainfall. This study is the first survey of the region of Caatinga in Paraiba state using active collecting, these results will serve to enrich the data to local forensic entomology and will contribute to the understanding the fauna of the region carrion flies.
Objetivou-se com este estudo inventariar e analisar a abundancia de dípteros da família Calliphoridae presentes ao longo da decomposição da carcaça de suínos na região do Cariri, estado da Paraíba, Brasil, em dois períodos: chuvoso e seco. O estudo foi desenvolvido na Reserva Particular do Patrimônio Natural (RPPN) na Fazenda Almas, no município de São José dos Cordeiros-PB. Foram utilizadas duas carcaças de suíno (Sus scrofa) por período, protegidas de animais carniceiros por uma gaiola metálica no interior de armadilha do tipo “Shannon”. As coletas foram realizadas diariamente, duas vezes ao dia, durante onze dias em ambos os períodos. Os dados meteorológicos de precipitação foram obtidos no Centro de Previsão de Tempo e de Estudos Climáticos do INMET e a temperatura e umidade relativa do ar foram obtidas in loco utilizando-se um termohigrômetro digital. Os insetos coletados foram encaminhados mediante autorização ao laboratório de entomologia da UFPB e posteriormente identificados e parte deles depositada na coleção de referencia. Foram coletados 8.811 indivíduos da família Calliphoridae, pertencentes a seis espécies: Chrysomya albiceps (Wiedemann, 1819), Cochliomyia macellaria (Fabricius, 1775), Chrysomya putoria (Wiedemann, 1818), Chloroprocta idioidea (Robineau-Devoidy, 1830), Chrysomya megacephala (Fabricius, 1794) e Lucilia eximia (Wiedemann, 1818), as quais estiveram presentes em ambos os períodos, porém a abundância relativa de espécies foi maior no período chuvoso (4.706/53,4%). Verificou-se a abundância de algumas espécies por período, onde a C. albiceps foi a espécie mais abundante no período seco (3.244/36,81%) e C. idioidea no período chuvoso (2.860/32,45%). Observaram-se cinco fases de decomposição: fresca, cromática, enfisematosa, coliquativa e esqueletização. A enfisematosa foi a fase onde mais se coletou Calliphoridae (3.923/44,52%), seguida pela esqueletização (3.458/39,25%), cromática (726/8,24%), coliquativa (627/7,12%) e fresca (77/0,87%). Constatou-se também que a maior abundancia de C. idioidea ocorreu no dia de maior elevação da umidade relativa e precipitação. Este é o primeiro estudo de levantamento realizado com coleta ativa em região de Caatinga paraibana, cujos resultados permitirão enriquecer os dados da entomologia forense local e contribuirão para o entendimento da fauna de dípteros necrófagos da região.
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Moretto, Maurício. "Comparação em estudo cego da aplicação de método antropométrico versus tabela de decisão LAF/CEMEL para estimativa de sexo em ossadas com perfil bioantropológico conhecido." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17143/tde-06062017-160210/.
Анотація:
A Antropologia Forense atua em análises dos mais diversos aspectos do ser humano aplicadas no interesse da Justiça. Na prática, atua na análise de restos humanos totalmente ou parcialmente esqueletizados no contexto de uma investigação, visando auxiliar ou até mesmo fornecer a identificação de cadáveres desconhecidos. Um dos poucos centros da especialidade no Brasil, o CEMEL baseia suas análises num protocolo de tomada de decisões, chamado LAF/CEMEL, elaborado desde 2005, que vem dando bons resultados, com níveis de identificação de ossadas na faixa de 38%. O protocolo é baseado em método de tabelas de decisões, analisando aspectos da anatomia óssea que permitam a classificação em sexo, ancestralidade, idade, estatura, dominância (destro/sinistro). No que tange à definição de sexo, baseia-se em informações de detalhes anatômicos principalmente da pelve, crânio e mandíbula. Elaborado em conjunto com pesquisadores da University of Sheffield (UK), no início se baseou em comparações com populações definidas, não miscigenadas: caucasiana, africana ou indígena/oriental. Porém, devido à miscigenação intensa da população brasileira, o protocolo LAF/CEMEL teve que ser adaptado, visando a melhora dos resultados. Da mesma forma, o que se tem atualmente em questão de análises antropométricas são dados da literatura mundial, na maioria das vezes estabelecidos para populações homogêneas com ancestralidade definida. Também nesse caso, há que se levar em conta a miscigenação, e até que ponto o método é eficaz ou, em associação com o protocolo atual, poderia torná-lo mais eficaz. Neste estudo foi proposta a avaliação de eficiência e eficácia do método antropométrico do triângulo do processo mastoide (porion, asterion e mastoidale) para estimativa de sexo, como descrito nos trabalhos de Kemkes e Göbel (2006) e Paiva e Segre (2003. Foram selecionados 74 crânios de ossadas sob a guarda do CEMEL/FMRP-USP. Destes, 28 foram excluídos por impossibilidade de execução do método antropométrico. Os 46 crânios restantes foram analisados, na forma de estudo cego, por três vezes pela antropometria e os resultados comparados entre si, assim como ao método do Protocolo LAF/CEMEL baseado em tomada de decisão. Os resultados demonstraram que, além do alto número de exclusões por impossibilidade de execução e da necessidade de refinamento técnico para evitar erros intraobservador, com imprecisão de até 16,9% e inexatidão de 67,4%, as somatórias das áreas obtidas contrastaram com os valores encontrados por Paiva e Segre (2003) em 60,9% dos casos. Conclui-se que, com o passar do tempo e refinamento técnico, o método antropométrico torna-se mais eficiente no que diz respeito à diminuição de erros do observador. Por outro lado, mostra-se ineficaz em estimar o sexo dos crânios analisados em amostra heterogênea (de população miscigenada), indicando o método de tomada de decisão como mais adequado.Forensic Anthropology deals with the analysis of various aspects of the human being applied to the interests of justice. In practice, it works with skeletonized or partially skeletonized human remains in the context of an investigation, aiming to help or even provide the identification of unknown corpses. One of the few centers of expertise in Brazil, CEMEL bases its analysis on a decision-making protocol, named LAF/CEMEL, developed since 2005, which has given good results, reaching identification levels in the 38% range. The protocol is based on method of decision tables, analyzing aspects of bone anatomy to allow classification of sex, ancestry, age, height, dominance (right-handed/sinister). Regarding the estimation of sex, it is based on anatomical information especially of the pelvis, skull and jaw. Prepared in cooperation with researchers from the University of Sheffield (UK), it was based on comparisons with defined populations: Caucasian, African or Indigenous/Eastern. However, due to the intense miscegenation of the Brazilian population, the LAF/CEMEL protocol had to be adapted, aiming at the improvement of the results. However, the questions are if anthropometric analysis as presented in the literature, originated from and applied for homogeneous populations of defined ancestry could be used for Brazilian cases, considering its admixed ancestry and to what extent the method is effective or, in association with the current protocol, could make it more effective. This study was proposed to evaluate the efficiency and effectiveness of an anthropometric method of sex estimation from the mastoid process triangle (porion, asterion and mastoidale), as described by Kemkes and Göbel (2006) and Paiva and Segre (2003). Of 74 skulls selected from the CEMEL/FMRP-USP collection, 28 were excluded due to the impossibility of execution of the method. The remaining 46 skulls were measured three times and the results compared with each other, as well as with the assignments based on the classifications used in the LAF/CEMEL protocol. In addition to the number of exclusions due to impossibility of execution of the method and the need for technical refinements to avoid intra-observer errors, the imprecision reached until 16,9% and inaccuracy 67,4%. The obtained results disagreed with those obtained by Paiva and Segre (2003) in 60.9% of cases. Reduction of observer errors over time and with technical refinement led to the anthropometric method increasing in efficiency. It was not as effective in estimating the sex of the skulls in the heterogeneous admixed sample compared to the anthroposcopic methods of LAF/CEMEL, indicating that these are more useful on samples of this kind.
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Alfazil, Abdulkareem Abdulwahab. "Stability of drugs and pesticides of forensic toxicological interest and their metabolites in biological samples." Thesis, University of Glasgow, 2009. http://theses.gla.ac.uk/1309/.
Анотація:
Loss of analyte from biological samples during the post-mortem interval or during storage has potentially serious implications in forensic toxicology and represents a challenge for the forensic toxicologist, especially in the interpretation of case results. The initial aim of the studies in this thesis was to evaluate the stability of some important drugs and compounds in blood under different storage conditions in order to optimize the preservation of these compounds. A second aim was to evaluate a new method of stabilizing these compounds in blood by storing them as dried blood spots on filter paper. The third aim was to investigate methods by which corrections could be made for analyte losses based on quantification of their degradation products, which would serve as markers of the former presence of the compounds even if they were no longer detectable. The background to toxicology and its classification systems is reviewed along with the most common areas of application, including forensic toxicology. Details are given of the most commonly-used matrices and of current problems facing forensic toxicologists, particularly the problem of analyte instability. The literature concerning stability of drugs and pesticides in biological samples are reviewed and discussed as well as methods applied to enhance and stabilize analytes for long storage periods. Background is provided on methodologies used in the work reported in this thesis, including extraction techniques and instrumental analysis by LC-MS/MS and GC/MS. Also, because of its importance in forensic toxicology at present validation procedures and requirements are also discussed. An initial study was made of drug stability during storage in blood samples for 1 year under conventional laboratory conditions using selected drugs from the benzodiazepine group, alprazolam, lorazepam, oxazepam and estazolam. Blank blood containing these drugs at low and high concentrations was stored in tubes at -20° C, 4°C and room temperature. Half of the tubes contained fluoride-oxalate preservative. Blood samples were analysed on the first (day zero), second and fourth days, and after one week, two weeks, one month, two months, three months, six months and one year using a method which was developed and validated for this study based on solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Alprazolam and estazolam were stable at -20° C and 4° C, but decreased by almost 10% at room temperature (RT) at both concentrations. Lorazepam and oxazepam were stable at 20°C but were poorly stable at 4° C and decreased by 100% at RT by the end of the 1 year period. Sodium fluoride stabilised the drugs by approximately 13% compared to unpreserved samples. The long-term stability of alprazolam and estazolam is attributed to the presence of the trizolo ring in their structures which makes the compounds more resistant to hydrolysis, the most prominent degradation reaction affecting benzodiazepines. A similar study was performed on the stability of morphine-3- and 6-glucuronide and codeine-6-glucuronide in blood and urine under the same storage conditions. These compounds were stable at -20° C, losing less than 7% but losses were higher at 4° C, up to 18% in blood and 28% in urine, and at room temperature up to 54% in blood and 78% in urine after 1 year. Sodium fluoride did not have a significant effect (<10% increase in stability). An investigation was carried out on stabilisation of hydrolytically-labile benzodiazepines and cocaine in blood during storage as dried blood spots (DBS) on filter paper. An analytical method was developed and validated for this study based on SPE and LC-MS/MS analysis. The drugs selected were flunitrazepam, temazepam, oxazepam, lorazepam, nitrazepam, diazepam and cocaine. Blood spots (100 µl blood) on Guthrie card 903 containing the drugs at 1000ng/ml were dried overnight at RT. Spots were cut out and extracted with buffer (pH 6), which was analysed with the validated method. DBS were stored in duplicate at RT, 4°C and -20°C for up to one year. Degradation of the drugs in DBS in all storage conditions was less than for the corresponding liquid blood samples stored under similar conditions. More than 80% of each analyte could be recovered from DBS after one month while 15 % cocaine and 74 % of the benzodiazepines were recovered after 1 year under all conditions. The degradation of diazepam, temazepam, chlorodiazepoxide and oxazepam by hydrolysis was studied over a 1 month period under conditions designed to accelerate the reaction (80 °C, pH 2 and 12) and the hydrolysis products 2-methylamino 5-chlorobenzophenone (MACB) and 2-amino 5-chlorobenzophenone (ACB) were analysed by a method based on SPE and LC-MS/MS which was developed and validated for this study. MACB and ACB in whole blood and urine were evaluated as indicators of the original drug concentrations. Blank blood and urine containing these compounds at 1000 ng/ml stored at high temperature (80°C) and under acidic (pH 2) and basic (pH 12) conditions at room temperature for one month. The samples were analyzed in duplicate at days 1, 2, 4, 7, 14 and 30. MACB and ACB were the main hydrolysis products and their concentrations increased as degradation of the drugs proceeded. They could be detected when the starting materials had completely disappeared. However, MACB and ACB were found to be further degraded under some of the conditions used and a further study was made of the conversion of MACB to ACB. It was concluded that the drugs studied were more sensitive to alkaline pH than to acidic pH or high temperature and that MACB and ACB can be used to confirm the original presence of these drugs in samples, especially when they have decomposed due to poor or prolonged storage conditions. A final study was made of organophosphates (OPs) and their dialkylphosphate (DAP) hydrolysis products. A new method was developed and validated for analysis of OPs and DAPs in blood samples based on SPE and GCMS after derivatization with N-tert-butyldimethylsilyl-N-methyltrifluroacetamide. The influence of sodium fluoride preservative and storage as DBS on filter paper on the stability of OPs in blood was assessed over a 3 day period at RT. With preservative, DAPs concentrations increased as degradation of the OPs proceeded and they could be detected when the parent compounds had completely disappeared. OPs in DBS showed good stability in comparison to liquid blood samples containing NAF and the parent compounds were detected at the end of the observation period. It was concluded that careful attention should be given to the storage of samples to avoid loss of analyte and erroneous interpretation of results. DBS could be an effective and inexpensive way of increasing analyte retention but routine use of preservatives without evaluation of their effects is discouraged, as these may accelerate loss of analyte.
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Battah, Abdel-Kader Hamdi. "The analysis of drugs and solvents in forensic toxicology by combined GC- and LC-MS." Thesis, University of Glasgow, 1989. http://theses.gla.ac.uk/4115/.
Анотація:
The work described in this thesis was in the field of Forensic Toxicology, which is the study and practice of toxicology for legal purposes. Three different application areas were considered: (a) the analysis of drugs in blood by gas chromatography-mass spectrometry (GC-MS) following isolation from the biological matrix by solid phase extraction procedures, (b) the analysis of paint solvents in blood by gas chromatography (GC) and GC-MS following dynamic headspace elution from blood, and (c) an evaluation of Thermospray/Plasmaspray (TSP/PSP LC-MS) liquid chromatography-mass spectrometry as the basis of drug screening techniques in forensic toxicology. (a) In the first of these areas, a sensitive, specific and reliable method was developed for the analysis of basic drugs in blood, using morphine and buprenorphine as model compounds which are also drugs commonly abused in the Glasgow area. A novel extraction procedure was used, based on a commercially available chemically-modified silica containing surface-bound benzenesulphonylpropyl groups, which served as a cation exchange resin. Several methods for the initial treatment of the biological matrix wre examined and the one selected involved absorption of the blood sample on diatomaceous earth and solvent elution of the crude extract containing any drugs present in the sample. This extract was then purified using the cation exchange resin: conditions suitable for the efficient retention of basic drugs and subsequent elution were also examined and developed. The extraction efficiencies for morphine were 925% and 954% at concentrations of 35 and 560ng/ml blood, respectively, for buprenorphine 836% and 875% , at concentration of 0.5 and 8ng/ml respectively, and for other drugs were better than 85% . The end-step analytical technique chosen for this method was GC-MS, because of analytical and legal requirements with respect to sensitivity and specificity. The polar nature of the model compounds, and of many other drugs likely to be encountered in this field, required chemical modification of the substances prior to gas chromatography. A comparison was made of three silyl ether derivatives - the trimethylsily (TMS), ethyldimethylsilyl (EMS) and tert-butyldimethylsilyl (MTBS) ethers. The conditions required for derivative formation were examined, including the choice of silyl donor reagent, solvents, temperature of reaction and time to completion. The EDMS donor, diethyltetramethyldisilazane, was selected on the basis of the midl reaction conditions required for the test compounds and also because this reagent caused less interference during GC-MS analysis. EDMS ethers gave satisfactory gas chromatographic behaviour and the presence of prominent ions at high mass was shown to be advantageous for specific and sensitive detection by SIR-MS with little background interference. The developed method was considered to be useful for both clinical and post-mortem blood samples containing morphine and buprenorphine down to the low picogram/millilitre level, and therefore adequate for the intended purpose. The method was subsequently applied to 13 samples submitted for analysis to the Department of Forensic Medicine and Science and found to be satisfactory. (b) In the second area of application, dynamic head space (DHS) elution was used for the analysis of paint solvents in blood. Volatilised solvents were trapped on a Tenax-GC cartridge and were subsequently analysed by GC-MS. The extraction efficiency of this method for C18-C12 n-alkane hydrocarbons, which were present in the paint materials, was better than 90% and sensitivity down to pg/ml levels was obtained. Solvent extraction procedures for these hydrocarbons were evaluated using high purity solvent. The extraction efficiency was better than 85% . Analysis of extracts by GC or GC-MS suffered from interference from the solvent front which reduced the sensitivity. The DHS method was applied to a pilot study for occupational monitoring of a group of painters to assess the presence of paint solvents in their blood. Two venous blood samples were collected at the beginning and at the end of a working week from each subject. They showed the presence of several solvents similar to those present in paint material and the levels in the second series of samples were higher than those of the first series. The differences between the levels in the two series were statistically significant for n-nonane, n-undecane and alkylbenzenes. The levels in the first sample indicated incomplete clearance of these solvents from the body during the weekend, and the second samples indicated solvent uptake during the working week. Solvent contamination in the extraction system was tackled by several approaches but still hindered the accurate estimation of solvent levels in blood. (c) In the third area of application, the operating parameters which control the sensitivity of the mass spectrometer using the TSP/PSP LC-MS interface were evaluated. These included the effects of the probe temperature and discharge voltage on sensitivity and mass spectral fragmentation pattern and the effects of the mobile phase constituents on sensitivity and mass spectral peak stability. Solvent systems containing ammonium acetate buffer and an organic modifier such as acetonitrile produced the best results in plasmaspray LC-MS. Three model HPLC-MS analyses were developed for mixtures of basic drugs, barbiturates and opiates using both the plasmaspray positive and negative ion modes. During the development of the mobile phases, the optimization of chromatography by organic modifiers was assessed. The quality of chromatography obtained was not always as good as expected in conventional HPLC, but the combination of chromatographic and mass spectral data could be used for identification and quantification purposes. A compilation of PSP mass spectra of drugs commonly encountered in forensic toxicology was produced. These mass spectra provided mostly molecular weight information with little structural information.
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Gettings, Katherine Butler. "Forensic Ancestry and Phenotype SNP Analysis and Integration with Established Forensic Markers." Thesis, The George Washington University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3590467.
Анотація:
When an evidential DNA profile does not match identified suspects or profiles from available databases, further DNA analyses targeted at inferring the possible ancestral origin and phenotypic characteristics of the perpetrator could yield valuable information. Single Nucleotide Polymorphisms (SNPs), the most common form of genetic polymorphisms, have alleles associated with specific populations and/or correlated to physical characteristics. With this research, single base primer extension (SBE) technology was used to develop a 50 SNP assay designed to predict ancestry among the primary U.S. populations (African American, East Asian, European, and Hispanic/Native American), as well as pigmentation phenotype. The assay has been optimized to a sensitivity level comparable to current forensic DNA analyses, and has shown robust performance on forensic-type samples. In addition, three prediction models were developed and evaluated for ancestry in the U.S. population, and two models were compared for eye color prediction, with the best models and interpretation guidelines yielding correct information for 98% and 100% of samples, respectively. Also, because data from additional DNA markers (STR, mitochondrial and/or Y chromosome DNA) may be available for a forensic evidence sample, the possibility of including this data in the ancestry prediction was evaluated, resulting in an improved prediction with the inclusion of STR data and decreased performance when including mitochondrial or Y chromosome data. Lastly, the possibility of using next-generation sequencing (NGS) to genotype forensic STRs (and thus, the possibility of a multimarker multiplex incorporating all forensic markers) was evaluated on a new platform, with results showing the technology incapable of meeting the needs of the forensic community at this time.
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Flemons, Kristin. "Medical humanitarianism and its mutations: an ethnography of the African Medical and Research Foundation." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119569.
Анотація:
This thesis examines the ways in which the African Medical and Research Foundation (AMREF) may be considered a 'mutation' of medical humanitarianism, across both time (through the course of the organization's history) and space (from humanitarianism's birthplace in Europe to AMREF's headquarters in East Africa). To this end, the author conducted field research with staff at AMREF's Kenya Country Office between June and September, 2012, involving participant observation, field visits, and archival research. In Chapter 1, the Foundation's commitment to projects of medical, technical and bureaucratic modernity in East Africa is explored, which complicates analyses of humanitarian minimalism. In Chapter 2, the organization's history is discussed as a reference point for understanding its unique characteristics. In Chapter 3, AMREF's research activities are unpacked as additions to, and transgressions of, boundaries between medical humanitarianism and global health.Ce mémoire cherche à montrer que l'African Medical and Research Foundation (AMREF) constitue une « mutation » de l'humanitaire médical, aussi bien dans le temps (à travers l'histoire de l'organisation) que dans l'espace (de l'Europe, lieu d'origine de l'humanitaire jusqu'au siège social d'AMREF en Afrique de l'Est). Dans le cadre de ce projet, l'auteure a entrepris quatre mois de recherches de terrain auprès du personnel des bureaux d'AMREF au Kenya, entre Juin et Septembre 2012, faisant usage de méthodes de recherche telles que l'observation participante, des visites de terrains, et de la recherche d'archives. Le premier chapitre documente l'engagement de la Fondation vis-à- vis de projets de modernité médicale, technique et bureaucratique en Afrique de l'Est, venant ainsi nuancer certaines analyses centrées sur le minimalisme humanitarisme. Dans le deuxième chapitre, une présentation détaillée de l'histoire de l'organisation permet d'en mettre en valeur son caractère particulier et unique. Enfin, dans un troisième chapitre, les activités de recherches d'AMREF sont analysées à la fois comme suppléments et transgressions des frontières entre l'humanitaire médical et la santé mondiale.
48
Whatson, Chloe Louise. "An examination of current practice and new developments in the forensic assessment of offenders." Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/6670/.
Анотація:
Exploring and understanding the practice of accurately assessing offenders is an important area of research for forensic practice and the risk management of offenders. This thesis aims to explore different approaches to offender assessment, specifically their clinical utility in forensic mental health settings. The first chapter, provides an introduction to the importance of accurate offender assessment, and presents current models proposed within the literature. The second chapter, provides a systematic review of historically used approaches in the assessment of risk, and compares their predictive accuracy and clinical utility in relation to a mentally disordered offending population. The third chapter, critically appraises a widely used assessment tool to assess risk within forensic mental health settings, the HCR-20 (Version 3.0; Douglas, Hart, Webster & Belfrage, 2013). The fourth chapter, moves way from specific tools used in current practice and instead explores clinicians current understanding and use of newer concepts in offender assessment (IOW/IOA), through a qualitative research study. Finally, the fifth chapter presents a thorough discussion of the overall content, findings and conclusions of this thesis. Findings have important implications for the practice of assessing and managing offenders effectively.
49
Al-Asmari, Ahmed Ibrahim. "Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites." Thesis, University of Glasgow, 2009. http://theses.gla.ac.uk/1290/.
Анотація:
This thesis studied opioids and alcohol in forensic toxicology by LC-MS/MS, which avoids time-consuming procedures involving hydrolysis, extraction and derivatisation. Initially, a method was validated for quantification of opioids and unhydrolysed polar metabolites in autopsy specimens and was used to develop procedures for interpretation of forensic toxicology results. The LC-MS/MS method developed has been validated for the simultaneous determination of 24 opioids in human whole blood, including, for the first time in human whole blood, naloxone-3-glucuronide. Although a large number of drugs of interest were included in the method, acceptance criteria for linearity, precision, and recovery for all analytes were achieved. The method was found useful for differentiating between users of heroin and other opioids, such as codeine and morphine, and for determining the survival time in deaths attributed to heroin use. Subsequently, the efficiencies of hydrolytic and non-hydrolytic methods for opioid analysis were compared for buprenorphine (BUP) analysis. The aims were to develop and validate a method for the direct determination (DM) of buprenorphine (BUP), norbuprenorphine (NBUB), buprenorphine-3-glucuronide (B3G) and norbuprenorphine-3-glucuronide (NBUP3G). This method was compared with an in house enzymatic hydrolysis method (HM) for the determination of total buprenorphine (TBUP) and norbuprenorphine (TNBUP), using real positive BUP urine case samples. A comparison between the drug and metabolite concentrations obtained by direct and hydrolysis methods was reported for the first time in this work. LC-MS analysis was also applied to paediatric plasma specimens obtained from a clinical pharmacokinetic study of intravenous and intranasal administration of diamorphine. This work was aimed at obtaining pharmacokinetic data for diamorphine and its metabolites in children following intravenous (IVDIM) and intranasal (INDIM) administration in a blind study. It was intended that the concentrations of active metabolites would be used to evaluate whether or not IN-DIM can deliver rapid and efficient analgesia in children comparable to that obtained with IV-DIM. The pharmacokinetics of DIM and its metabolites following INDIM and IVDIM administration in children have been compared for the first time in this study, which confirmed that INDIM can achieve therapeutic plasma concentrations of active metabolites, although these were lower than those obtained with IVDIM and occur at later times after administration. In Scotland, the number of prescriptions for oxycodone has risen by 430% since prescribing began in 2002. Blood samples from fatalities in the West of Scotland involving oxycodone were analysed using an LC-ESI-MS/MS method developed for the determination of oxycodone and its metabolites in post-mortem specimens. To the author’s knowledge, this is the first report of blood and urine concentrations of noroxycodone and oxymorphone in acute oxycodone overdoses. Also, it is the first LC-MS/MS application to be reported with oxycodone related fatalities cases in forensic toxicology as most of previous reports used GC or HPLC applications. Moreover, this work reported for the first time vitreous humour levels of noroxycodone following oxycodone intoxication. Ten oxycodone-related deaths were identified in the short period of this study in the Strathclyde region of Scotland alone, highlighting the importance of including this drug in routine laboratory screening and confirmation procedures. Polar alcohol metabolites ethyl glucuronide and ethyl sulfate are biomarkers of ante-mortem alcohol consumption and are used to test for post-mortem artefactual formation of alcohol. An LC-MS method for these metabolites using a novel hydrophilic interaction liquid chromatography column was validated and applied to routine forensic casework. Ninety urine case samples were divided into three groups depending on the ethanol concentration found in blood and analysed by the developed method: group A with post-mortem blood ethanol higher than 200 mg/100 mL; group B with ethanol concentration in the range 80 to 200 mg/100 mL and group C with ethanol concentration less than 80 mg/100 mL. It was concluded that the risk of false positive ethanol results increased in the low ethanol concentration group as several cases tested negative for both biomarkers. ETG was detected at low concentrations in some cases for which ETS tested negative, suggesting that either ETG may have a longer half-life in urine or else ETS is unstable. The data was compared with previous studies and confirmed that both ethanol biomarkers should be determined in heavily putrefied cases and when the ethanol level in post-mortem blood is low, suggesting the production of ethanol after death. To the authors’ knowledge, this is the first report of the determination of ETS using an LC-ESI-ion trap-MS/MS method, and of a HILIC-ESI-ion trap-MS/MS method for the simultaneous determination of ETG and ETS in post-mortem urine samples.
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Al-Ahmadi, Tareq Mohmmed. "A comparison of derivatisation procedures for the detection of multiple analytes in systematic forensic toxicology." Thesis, University of Glasgow, 2007. http://theses.gla.ac.uk/948/.
Анотація:
Three different derivatisation procedures were evaluated for their general applicability to systematic toxicological analysis (STA) involving (a) acylation with pentafluoropropionyl anhydride (PFPA) and pentafluoropropanol (PFP-OH), (b) acylation/esterification (methylation) with pentafluoropropionyl anhydride (PFPA) and a novel methylating agent trimethylsilyldiazomethane (TMS-diazomethane), used as a chromatographic derivatisation reagent for the first time in this study, and (c) silylation with tertiary-butyldimethylsilyl-trifluoroacetamide (MTBSTFA). Model compounds were selected for the evaluation process including a primary amine (amphetamine), a secondary amine (methamphetamine), alicyclic and aromatic hydroxy compounds (morphine, tetrahydrocannabinol), and carboxylic acids (benzoylecgonine, 11-nor tetrahydrocannabinol-9-carboxylic acid). For method (a) derivatisation was successful for all of the test compounds and mass spectra were obtained for each of them. For method (b), the novel methylating agent trimethylsilyl-diazomethane was used to convert carboxylic acids into the corresponding methyl esters. This reaction was found to proceed rapidly and quantitatively at room temperature and holds potential for future use in toxicology to replace diazomethane, a hazardous and toxic material. Method (c) gave derivatives with all test compounds except the secondary amine, methamphetamine, and the alcohol, morphine. The gas chromatographic behaviour of these derivatives was good and the mass spectra had prominent ions suitable for GC-MS-SIM. The extraction of multiple drugs from blood was evaluated using the novel polymeric SPE sorbent Strata-X. The same test compounds were used to assess the extraction step in terms of recovery and variation (within day and between days). The extracts were analysed by GC-MS-SIM using each of the three types of derivative. Recoveries of the test compounds were in the range of 50-100 percent depending on the analyte and its concentration in blood. All calibration curves were linear and had correlation coefficients higher than 0.99. Within day variations and between day variations were in the range of 2-22% relative standard deviation. Limits of detection and quantitation were measured for the model compounds and were found to be in the ranges of 0.4-7.3 ng/ml and 1.1-24.4 ng/ml respectively. The full method, combining extraction with each of the derivatisation reactions was finally evaluated for the presence of interferences with real case blood samples. The three derivatisation procedures were evaluated using four test compounds comprising diazepam plus its three metabolites nordiazepam, temazepam and oxazepam. The hydroxylated metabolites (temazepam and oxazepam) formed derivatives readily with all three reagent mixtures but nordiazepam (secondary aromatic amine) did not react except with MTBSTFA. Based on the work of this study it is concluded that a method is possible for STA based on a polymeric sorbent, to give a general extract, followed by a generalised derivatisation procedure such as acylation, with PFPA/PFP-OH prior to GC-MS.