Дисертації: "FMFC"

1

AHMED, DANIYAL. "Harvesting Energy from Microbial Fuel Cells and their Impedance Analysis." Doctoral thesis, Politecnico di Torino, 2019. http://hdl.handle.net/11583/2740593.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Aissioui, Abdelkader. "Le chemin vers les architectures futures des services mobiles : du Follow Me Cloud (FMC) au Follow Me edge Cloud (FMeC)." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLV095.

Повний текст джерела

Анотація:

Les travaux décrits dans cette thèse de doctorat visent à traiter les futures architectures de fourniture de services mobiles basés sur le cloud, à travers l'évolution des infrastructures réseau partant de Mobile Cloud Computing (MCC) au Mobile Edge Computing (MEC). Nous nous sommes essentiellement concentrés sur le concept Follow Me Cloud (FMC) comme une nouvelle stratégie de fourniture de services pour une meilleure expérience utilisateur et une utilisation efficace des ressources. Cela permet aux services basés sur le cloud de "suivre" leurs utilisateurs mobiles au cours de leurs déplacements à travers les technologies de réseau d'accès, tout en fournissant le service basé sur le cloud via le point de service le plus optimal au sein de l'infrastructure cloud. Plusieurs contributions sont proposées dans cette thèse, avec des évaluations à la fois en analyse théorique et en simulation scientifique.Premièrement, nous avons proposé une architecture alternative FMC qui permet: (i) d'ouvrir la conception FMC sur les technologies d'accès réseau mobile non-3GPP (ii) d'assurer l'interopérabilité entre différents domaines PMIPv6 permettant au MN une itinérance inter-domaines PMIPv6 avec une mobilité IP transparente ainsi qu'une continuité de session de service.(iii) d'offrir une architecture sans tunnel dans les situations d'itinérance de MN, en évitant ainsi toute surcharge supplémentaire liée aux tunnels dans la gestion de la mobilité. Le schéma proposé exploite la technologie SDN/OpenFlow et le protocole de gestion de la mobilité PMIPv6 en les intégrant dans un unique framework permettant de réaliser la vision FMC.Deuxièmement, pour aborder les problèmes d'évolutivité et de résilience dans les architectures SDN/OpenFlow centralisées de plan de contrôle, nous avons introduit une nouvelle conception d'un contrôleur SDN élastique et distribué adapté pour MCC et plus particulièrement pour les systèmes de gestion FMC. Nous avons illustré comment le nouveau schéma de plan de contrôle est distribué sur une architecture hiérarchique à deux niveaux, un premier niveau avec un seul contrôleur SDN global et un second niveau avec plusieurs contrôleurs SDN locaux. Ensuite, nous avons présenté les éléments constitutifs de notre nouvel framework de plan contrôle, le calcul de l'indicateur de performance (KPI) du système, et nous avons fixé l'objectif clé de notre conception visant à maintenir la valeur KPI du système dans une fenêtre de seuil prédéfinie. Enfin, nous avons démontré comment cet objectif est atteint en adaptant dynamiquement le nombre et l'emplacement des contrôleurs SDN locaux en utilisant la technologie NFV pour provisionner les contrôleurs SDN en tant que instances VNF (fonction réseau virtuelle) dans le cloud.Troisièmement, nous avons introduit le concept FMeC, exploitant les capacités offertes par la combinaison des architectures MEC et FMC dans le but de satisfaire aux exigences des systèmes automobiles 5G. Nous avons commencé par définir les éléments clés du concept FMeC permettant de fournir la technologie FMC en bordure des réseaux mobiles. Ensuite, nous avons présenté une projection de notre solution FMeC sur un cas d'utilisation de conduite automatisée intégrant l'industrie automobile aux infrastructures Telecom en vue de la vision automobile 5G future. Avec une focalisation sur les types de communications V2I/N, nous avons présenté la conception de notre architecture FMeC basée sur les technologies SDN/OpenFlow et les entités de l'infrastructure MEC dont les ressources sont mises en commun pour fournir un cloud de bordure fédéré. Enfin, nous avons présenté notre framework sensible à la mobilité pour le placement des services dans le cloud de bordure, ce dernier est fondé sur un ensemble d'algorithmes de base qui permettent d'atteindre les exigences de QoS de la conduite automatisée en termes de latence ultra-courte au sein du réseau 5G
This Ph.D. thesis aims to deal with the future delivery architectures of mobile cloud-based services, through network infrastructures evolving from Mobile Cloud Computing (MCC) to Mobile Edge Computing (MEC). We mainly focused on Follow Me Cloud (FMC) concept as a new service delivery strategy for improved user experience and efficient resource utilization. That enables cloud-based services to follow their mobile users during their movement across access network technologies and by delivering the cloud-service via the optimal service point inside the cloud infrastructure. Several contributions are proposed in this thesis and evaluated in both theoretical analysis and scientific simulation.First, we proposed an alternative FMC architecture that allows: (i) to open the FMC design on non-3GPP mobile network access technologies (ii) to provide interoperability among different PMIPv6 domains permitting MNs inter-PMIPv6 domain roaming with seamless IP mobility and service session continuity (iii) to offer a tunnel-free architecture in MNs roaming situation, avoiding any additional overhead associated with tunneling in mobility management. This proposed scheme leverage SDN/OpenFlow technology and PMIPv6 mobility management protocol by integrating them within a framework permitting to realize the FMC vision.Second, to address the scalability and resiliency concerns in centralized SDN/OpenFlow control plane architecture, we introduced a new design of an elastic distributed SDN controller tailored for Mobile Cloud Computing (MCC) and more notably for Follow Me Cloud (FMC) management systems. We illustrated how the new control plane scheme is distributed on two-level hierarchical architecture, a first level with a single global SDN controller and a second level with several local SDN controllers. Then, we presented the building blocks of our novel control plane framework, the system Key Performance Indicator (KPI) computation and set the key objective of our design aiming to keep the system KPI value within a predefined threshold window. Last, we proved how this goal is achieved by adapting the number of local SDN controllers and their locations in an elastic manner and deploying them as VNF instances on the cloud thanks to NFV technology.Third, we introduced FMeC concept, leveraging the intertwining of MEC and FMC architectures with the aim of sustaining requirements of the 5G automotive systems. We began by defining FMeC key concept elements permitting to provide FMC technology at the edge of mobile networks. Then, we presented an automated driving use case projection of our FMeC solution integrating automotive with Telco infrastructures towards the future 5G automotive vision. Focusing on the V2I/N communications types, we introduced our FMeC design architecture based on SDN/OpenFlow technologies and MEC infrastructure entities whose resources are pooled together to provide a federated edge clouds. Finally, we presented our mobility-aware framework for edge-cloud service placement based on a set of basic algorithms that permit achieving the automated driving QoS requirements in terms of ultra-short latency within 5G network

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Jayawarna, Vineetha. "Fmoc-peptide gels for cell culture." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489519.

Повний текст джерела

Анотація:

Spontaneous formation of macroscopic hydrogels from small molecule building blocks via self-assembly is a powerful tool for the preparation of novel materials with well defined properties. Peptides are particularly interesting as building blocks for these materials. Self-assembled nanowires, fibres, sheets and tubes from peptide systems have all been described.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Aufderhorst-Roberts, Anders. "Microrheological characterisation of Fmoc derivative hydrogels." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608155.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Zhou, Mi. "Self-Assembled bioactive fmoc-peptide hydrogels for skin tissue engineering." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509392.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Kluth, Stephan. "Quantitative modeling and analysis with FMC-QE." Phd thesis, Universität Potsdam, 2011. http://opus.kobv.de/ubp/volltexte/2011/5298/.

Повний текст джерела

Анотація:

The modeling and evaluation calculus FMC-QE, the Fundamental Modeling Concepts for Quanti-tative Evaluation [1], extends the Fundamental Modeling Concepts (FMC) for performance modeling and prediction. In this new methodology, the hierarchical service requests are in the main focus, because they are the origin of every service provisioning process. Similar to physics, these service requests are a tuple of value and unit, which enables hierarchical service request transformations at the hierarchical borders and therefore the hierarchical modeling. Through reducing the model complexity of the models by decomposing the system in different hierarchical views, the distinction between operational and control states and the calculation of the performance values on the assumption of the steady state, FMC-QE has a scalable applica-bility on complex systems. According to FMC, the system is modeled in a 3-dimensional hierarchical representation space, where system performance parameters are described in three arbitrarily fine-grained hierarchi-cal bipartite diagrams. The hierarchical service request structures are modeled in Entity Relationship Diagrams. The static server structures, divided into logical and real servers, are de-scribed as Block Diagrams. The dynamic behavior and the control structures are specified as Petri Nets, more precisely Colored Time Augmented Petri Nets. From the structures and pa-rameters of the performance model, a hierarchical set of equations is derived. The calculation of the performance values is done on the assumption of stationary processes and is based on fundamental laws of the performance analysis: Little's Law and the Forced Traffic Flow Law. Little's Law is used within the different hierarchical levels (horizontal) and the Forced Traffic Flow Law is the key to the dependencies among the hierarchical levels (vertical). This calculation is suitable for complex models and allows a fast (re-)calculation of different performance scenarios in order to support development and configuration decisions. Within the Research Group Zorn at the Hasso Plattner Institute, the work is embedded in a broader research in the development of FMC-QE. While this work is concentrated on the theoretical background, description and definition of the methodology as well as the extension and validation of the applicability, other topics are in the development of an FMC-QE modeling and evaluation tool and the usage of FMC-QE in the design of an adaptive transport layer in order to fulfill Quality of Service and Service Level Agreements in volatile service based environments. This thesis contains a state-of-the-art, the description of FMC-QE as well as extensions of FMC-QE in representative general models and case studies. In the state-of-the-art part of the thesis in chapter 2, an overview on existing Queueing Theory and Time Augmented Petri Net models and other quantitative modeling and evaluation languages and methodologies is given. Also other hierarchical quantitative modeling frameworks will be considered. The description of FMC-QE in chapter 3 consists of a summary of the foundations of FMC-QE, basic definitions, the graphical notations, the FMC-QE Calculus and the modeling of open queueing networks as an introductory example. The extensions of FMC-QE in chapter 4 consist of the integration of the summation method in order to support the handling of closed networks and the modeling of multiclass and semaphore scenarios. Furthermore, FMC-QE is compared to other performance modeling and evaluation approaches. In the case study part in chapter 5, proof-of-concept examples, like the modeling of a service based search portal, a service based SAP NetWeaver application and the Axis2 Web service framework will be provided. Finally, conclusions are given by a summary of contributions and an outlook on future work in chapter 6. [1] Werner Zorn. FMC-QE - A New Approach in Quantitative Modeling. In Hamid R. Arabnia, editor, Procee-dings of the International Conference on Modeling, Simulation and Visualization Methods (MSV 2007) within WorldComp ’07, pages 280 – 287, Las Vegas, NV, USA, June 2007. CSREA Press. ISBN 1-60132-029-9.
FMC-QE (Fundamental Modeling Concepts for Quantitative Evaluation [1]) ist eine auf FMC, den Fundamental Modeling Concepts, basierende Methodik zur Modellierung des Leistungsverhaltens von Systemen mit einem dazugehörenden Kalkül zur Erstellung von Leistungsvorhersagen wie Antwortzeiten und Durchsatz. In dieser neuen Methodik steht die Modellierung der hierarchischen Bedienanforderungen im Mittelpunkt, da sie der Ursprung aller dienstbasierenden Systeme sind. Wie in der Physik sind in FMC-QE die Bedienanforderungen Tupel aus Wert und Einheit, um Auftragstransformationen an Hierarchiegrenzen zu ermöglichen. Da die Komplexität durch eine Dekomposition in mehreren Sichten und in verschiedene hierarchische Schichten, die Unterscheidung von Operations- und Kontrollzuständen, sowie dazugehörige Berechungen unter Annahme der Stationarität reduziert wird, skaliert die Anwendbarkeit von FMC-QE auf komplexe Systeme. Gemäß FMC wird das zu modellierende System in einem 3-dimensionalen hierarchischen Beschreibungsraum dargestellt. Die quantitativen Kenngrößen der Systeme werden in drei beliebig frei-granularen hierarchischen bi-partiten Graphen beschrieben. Die hierarchische Struktur der Bedienanforderungen wird in Entity Relationship Diagrammen beschrieben. Die statischen Bedienerstrukturen, unterteilt in logische und reale Bediener, sind in Aufbaudiagrammen erläutert. Außerdem werden Petri Netze, genauer Farbige Zeit-behaftete Petri Netze, dazu verwendet, die dynamischen Abläufe, sowie die Kontrollflüsse im System zu beschreiben. Anschließend wird eine Menge von hierarchischen Gleichungen von der Struktur und den Parametern des Modells abgeleitet. Diese Gleichungen, die auf dem stationären Zustand des Systems beruhen, basieren auf den beiden Fundamental Gesetzen der Leistungsanalyse, dem Gesetz von Little und dem Verkehrsflussgesetz. Das Gesetz von Little definiert hierbei Beziehungen innerhalb einer hierarchischen Schicht (horizontal) und das Verkehrsflussgesetz wiederum Beziehungen zwischen hierarchischen Schichten (vertikal). Die Berechungen erlauben Leistungsvorhersagen für komplexe Systeme durch eine effiziente Berechnung von Leistungsgrößen für eine große Auswahl von System- und Lastkonfigurationen. Innerhalb der Forschungsgruppe von Prof. Dr.-Ing Werner Zorn am Hasso Plattner Institut an der Universität Potsdam ist die vorliegende Arbeit in einen größeren Forschungskontext im Bereich FMC-QE eingebettet. Während hier ein Fokus auf dem theoretischen Hintergrund, der Beschreibung und der Definition der Methodik als auch der Anwendbarkeit und Erweiterung gelegt wurde, sind andere Arbeiten auf dem Gebiet der Entwicklung einer Anwendung zur Modellierung und Evaluierung von Systemen mit FMC-QE bzw. der Verwendung von FMC-QE zur Entwicklung einer adaptiven Transportschicht zur Einhaltung von Dienstgüten (Quality of Service) und Dienstvereinbarungen (Service Level Agreements) in volatilen dienstbasierten Systemen beheimatet. Diese Arbeit umfasst einen Einblick in den Stand der Technik, die Beschreibung von FMC-QE sowie die Weiterentwicklung von FMC-QE in repräsentativen allgemeinen Modellen und Fallstudien. Das Kapitel 2: Stand der Technik gibt einen Überblick über die Warteschlangentheorie, Zeit-behaftete Petri Netze, weitere Leistungsbeschreibungs- und Leistungsvorhersagungstechniken sowie die Verwendung von Hierarchien in Leistungsbeschreibungstechniken. Die Beschreibung von FMC-QE in Kapitel 3 enthält die Erläuterung der Grundlagen von FMC-QE, die Beschreibung einiger Grundannahmen, der graphischen Notation, dem mathematischen Modell und einem erläuternden Beispiel. In Kapitel 4: Erweiterungen von FMC-QE wird die Behandlung weiterer allgemeiner Modelle, wie die Modellklasse von geschlossenen Netzen, Synchronisierung und Mehrklassen-Modelle beschrieben. Außerdem wird FMC-QE mit dem Stand der Technik verglichen. In Kapitel 5 werden Machbarkeitsstudien beschrieben. Schließlich werden in Kapitel 6 eine Zusammenfassung und ein Ausblick gegeben. [1] Werner Zorn. FMC-QE - A New Approach in Quantitative Modeling. In Hamid R. Arabnia, editor, Proceedings of the International Conference on Modeling, Simulation and Visualization Methods (MSV 2007) within WorldComp ’07, 280 – 287, Las Vegas, NV, USA, Juni 2007. CSREA Press. ISBN 1-60132-029-9.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Melki, Cecilia, and Paola Rashid. "Standardisering VS Anpassning och korrelationen till lönsamhet : En flerfallsstudie om hur anpassningsgraden av marknadsstrategier påverkar prestation." Thesis, Södertörns högskola, Institutionen för ekonomi och företagande, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-14591.

Повний текст джерела

Анотація:

Syfte: Studien syftar till att undersöka förhållandet mellan standardisering och anpassning. Detta genom att undersöka prestationen för olika marknadsstrategier beroende på anpassningsgrad samt omvärldsfaktorer. Problemformulering: Hur korrelerar anpassningsgraden för marknadsstrategier till prestation? Metod: Kvantitativ och kvalitativ metod har används i första hand för att angripa vår problemformulering. Valet av ansats är viktigt för att kunna avgöra lämplig analysmetod. Det finns sedan två sätt att mäta hur verkligheten ser ut och det är genom induktion och deduktion. För att få en bra överskådlig bild har fallstudie varit en utmärkt val. Flerfallstudier består av flera fall och avsikten med en sådan studie är att få fram det säregna med huvudfallet genom att jämföra med andra fall. Dessa utgör vår population och därmed måste ett urval göras för att det inte skall bli för stort och det måste rymma inom vår tidsram. Totalt har 1102 produkter undersökt för sju olika varumärkena i tio länderna. Teorier: Lönsamhet och prestation är av stor vikt i uppsatsen eftersom beslutet för om en marknadsföringsstrategi ska standardiseras eller anpassas kommer i slutändan ned till den ekonomiska vinsten för företaget. Det finns tre övergripande synsätt, total standardisering, total anpassning eller att använda en mix av dessa. Vi har använt oss av AdaptStand process modellen, och det beror på att modellen visar hur företag bör gå till väga vid utformning av marknadsstrategier. Modellen är en kombination av standardisering och anpassning och utgör ett hjälpmedel för att finna vilken grad en strategi ska anpassas/standardiseras. Marknadsmixen, de fyra P:na, har haft en ledande roll i uppsatsen. Dock föll ett av P:na bort och det var plats. Anledningen till detta var på grund av att det var svårt att få tag på informationen som behövdes för att det skulle vara relevant för att besvara problemformuleringen. Slutsatser: För att man ska vara lönsam måste man finna en balans som är tillåten mellan marknaden man vill etablera sig i samt företagets kärna. man kan inte tillämpa en marknadsstrategi fullt ut, dvs. med en total anpassning eller total standardisering. Man måste visa för den nya marknaden att man tar hänsyn till deras behov och omständigheter samtidigt ska man inte ge för mycket av vad som kärnan i ens eget företag. Kärnan kan vara namnet, logo, produkterna, förpackningarna osv. Man måste hitta en balans som fungerar för båda parter om man vill vara långvarig och lönsam på den nya marknaden och slå igenom som en världsprodukt.
Objective: This study aims to examine the relationship between standardization and adaptation. This by examining the performance of different marketing strategies depending on the degree of adaptation and environmental factors Problem formulation: How to correlate the degree of adaptation marketing strategies to performance? Methodology: Quantitative and qualitative methods have been used primarily to attack our problem. The choice of approach is important to determine the appropriate method of analysis. There are then two ways to measure the reality and it is through induction and deduction. To get a good overall picture this methodology an excellent choise for our case. More Case Studies consists of a number of cases and the purpose of this study is to seek out the peculiarity of the main case by comparing with other cases. These are our population and therefore a choice hade to be made and that was to make sure that the population become too large and it must fit within our time frame. A total of 1102 products were investigated for seven different brands in ten countries. Theories: Productivity and performance are of great importance in the essay because the decision of whether a marketing strategy to standardize or adapt will ultimately down to the economic benefit for the company. There are three general approaches, total standardization, overall adjustment, or to use a mix of these. We have used the AdaptStand process model, and it is because the model shows how companies should design there marketing strategies. The model is a combination of standardization and adaptation, and as a tool for finding the degree to which a strategy will be adapted / standardized. Marketing mix of the four P's hade a leading role in the essey. However, one of the P´s fell away and there was place. The reason for this was because it was difficult to obtain information needed for it to be relevant to answer the problem formulation. Conclusions: In order to be profitable you have to find a balance that is allowed between the market they want to establish themselves in and the company's identity. You can not use a marketing strategies in full use which a total adaptation or total standardization. You have to show for the new market that the company takes their needs and circumstances in to consideration at the same time the companies should not give too much of what is their identity. The identity may be such as the name, logo, products, packaging and so on. One must find a balance that works for both parties if you want to be a lasting and profitable company in the new market and to make it as a world product.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Fjeld, Hanna. "Kartläggning av FMF och PFAPA hos barn i VästraGötalandsregionen." Thesis, Örebro universitet, Institutionen för medicinska vetenskaper, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-65409.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Mallette, Marielle. "Un programme de formation musicale créative (FMC) au secondaire /." Thèse, Chicoutimi : Université du Québec à Chicoutimi, 2002. http://theses.uqac.ca.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Vieira, João Paulo Fernandes 1982. "Separação enantiomérica do marcador molecular fmoc-poac em fase estacionária normal e reversa." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266863.

Повний текст джерела

Анотація:

Orientador: Cesar Costapinto Santana
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química
Made available in DSpace on 2018-08-19T02:22:30Z (GMT). No. of bitstreams: 1 Vieira_JoaoPauloFernandes_M.pdf: 2071458 bytes, checksum: 4e3166090a9b86f0b92b425b128fb73f (MD5) Previous issue date: 2011
Resumo: A enantiosseparação de alguns compostos é um brilhante e interessante tópico de muitas áreas da química analítica, principalmente na farmacêutica e biomédica. Sabe-se que apesar dos enantiômeros apresentarem fórmulas e massa molecular iguais, quando expostos em um ambiente biológico podem mostrar grandes diferenças nas suas atividades biológicas. O Fmoc-POAC (9-fluorenilmetiloxicarbonil -2,2,5,5-tetrametilpirrolidina-N-oxil-3-amino-4- ácido carboxílico) é um marcador paramagnético quiral com grande potencialidade de uso como derivado marcador de estruturas peptídicas com funções no organismo animal. De acordo com a literatura consultada, não há relatos de escalas semipreparativas na separação enantiomérica desse composto, extremamente necessária para testes de clínicos-analíticos. Este estudo teve como objetivo o desenvolvimento de métodos inovadores na separação enantiomérica do Fmoc-POAC e obtenção dos parâmetros necessários para um aumento de escala. O presente trabalho realizou uma avaliação experimental através de Cromatografia Líquida de Alta Eficiência (CLAE) para enantiosseparação desse composto, com eluição isocrática e nas colunas quirais de fase estacionária normal e reversa: i) analítica da OD-RH Chiralcel® (150x4,6 mm); ii) Analítica Lux Cellulose-2 da Phenomenex® (250x4,6 mm); iii) Semipreparativa OD da Chiralcel® (150x10 mm); iv) Semipreparativa OD-RH da Chiralcel® (250x21 mm). A partir desses ensaios experimentais, foram estimados os parâmetros cromatográficos da enantiosseparação do marcador molecular Fmoc-POAC nas colunas estudadas, além de parâmetros de transferência de massa e termodinâmicos. Os resultados desse trabalho foram que todas as colunas estudadas apresentaram a possibilidade de separação enantiomérica do Fmoc-POAC através desses métodos relativamente simples comparados aos apresentados na literatura, com destaque para a coluna de fase estacionária normal Lux Cellulose-2 (250x4,6 mm), com resolução de até 18,4. De acordo com resultados obtidos, temos a possibilidade de realizar a separação e recuperação desse composto, lançando-se mão de técnicas cromatográficas de maior rendimento, como sistemas contínuos de separação cromatográfica
Abstract: The enantioseparation of some compounds has interesting application in several areas of analytical chemistry, especially in pharmaceutical and biomedical. It is well known that some compounds with same chemical formulas and molecular mass, when exposed to a biological environment, may show different biological activities. The 9- fluorenilmetiloxicarbonil-2,2,5,5-tetrametilpirrolidina-N-oxil-3-amino-4-carboxylic acid (Fmoc-POAC) is a chiral paramagnetic marker with great potential as a marker for peptide structures with functions in the animal organism. According to the literature, there are no reports of enantiomeric separation of this compound unless in laboratory scale and large scale would be necessary for clinical and analytical testing. This study aimed at the development of innovative methods for the enantiomeric separation of Fmoc-POAC as well as obtaining the necessary parameters for scale up of their purification. The present work carried out an experimental evaluation using High Performance Liquid Chromatography (HPLC) for this compound enantioseparation with isocratic elution and columns with normal and reverse chiral stationary phase: i) Analytical Chiralcel® OD-RH (150x4, 6 mm ), ii) Analytical Lux Cellulose-2 from Phenomenex® (250x4, 6 mm), iii) Semi-preparative Chiralcel OD® (150x10 mm), iv) Semi-preparative Chiralcel OD-RH® (250x21 mm). From these assays, chromatographic parameters were estimated for the enantioseparation of Fmoc-POAC molecular marker beside parameters related to the thermodynamics and mass transfer. The conclusions in this research were that all columns present the possibility of enantiomeric separation of Fmoc-POAC by methods relatively simple compared to those presented in the literature, specially the column with normal stationary phase Lux Cellulose-2 (250x4, 6 mm) with resolution of up to 18.4. The results indicate the possibility of enantioseparation and recovery of these compounds by high yield continuous chromatography techniques
Mestrado
Desenvolvimento de Processos Biotecnologicos
Mestre em Engenharia Química

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Sasselli, Iván Ramos. "Modelling Fmoc-dipeptide nanostructures : the synergistic effect of combining computational and experimental methods." Thesis, University of Strathclyde, 2015. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=27047.

Повний текст джерела

Анотація:

Nanomaterials based on aromatic peptide amphiphiles are interesting new materials with potential applications in the areas of biomedicine and nanotechnology. These natural based materials take advantage of the properties of the peptides, as it is the ability to form the final structures spontaneously without any external stimulus by self-assembly, or the high number of functionality available due to the 20 natural building blocks, amino acids, and their possible combinations. Although it is known that the functionality of these nanostructures is highly dependent on both, the chemical groups and the topology of the nanostructure, and both vary with the amino acids side chains, the relationship between nanostructure shape and peptide chain composition is still unknown. Understanding this is necessary to be able to design Fmoc-peptide nanostructures on demand. In this thesis a combination between these experimental techniques and computational methods, molecular dynamic (MD) simulations, is used to elucidate the self-assembly motifs for a set of model systems composed of Fmoc-dipeptides. The interpretation of the experimental spectroscopic characterization is improved by using enzymatic self-assembly under thermodynamic control Fmoc-dipeptide and side-by-side comparison of nanostructures using dynamic peptide libraries (DPLs). This approach allowed to resolve which features increase the self-assembly tendency of these molecules. Both MD and DPL approaches depend on the premise that gels can be at thermodynamic equilibrium, which is not clear in the literature. It has been argued that they represent metastable states, where crystals are suggested to represent the actual thermodynamically favoured structures. Hence, the study starts with a model proposed to demonstrate that nanofibrous gels can represent the thermodynamically favoured structure. This is achieved by using a packing model where self-assembling molecules are represented by prisms with faces of different nature, solvophilic andsolvophobic to mimic the amphiphilicity of these molecules as a key feature. This approach gives rise to a combination of solvophobic and solvophilic interactions where a level of solvent exposure is favourable. The model depends on parameters which can be related with features of the system and demonstrates that the amphiphilicity is key to allow 1D objects, fibres, to be more stable than 3D objects,crystals; and hence, that MD simulations and DPLs can be applied for their study. For MD simulations, the CHARMM force field is used because it has beenapplied and validated to a wide variety of peptide-based systems. However, this forcefield does not include parameters for the Fmoc moiety. Therefore, the second steps for this study was to develop an Fmoc parameterization for the CHARMM force field, in order to be able to run all atoms self-assembling Fmoc-peptides simulations, to improve the understanding of these nanostructures and their formation. The parameterization is based in the CHARMM protocol adapted due to the amphiphilic nature of the Fmoc moiety. Experimentally, in order to get more valuable information from the experimental characterization, the study of different Fmoc-dipeptides nanostructures with specific changes in their peptide chain are compared in order to understand how these specific changes affect the self-assembled structure: phenylalanine/leucinesubstitution to understand how the aromatic side chain affects; and amide/methyl esterC-terminus substitution, to understand the role of the possible extra hydrogen bondsof the amide group. Furthermore, DPLs are also applied to rationalize the influence of these changes in the self-assembling tendency of Fmoc-dipeptides. Then, the experimental information is used to develop a model for Fmoc-TF-NH ₂ fibre and simulate it. The analysis of the model in addition with correlation of these data with the experimental insights, allows the refinement of the model. The resulting new model is validated by comparing the simulation analysis with the previous model and,again, correlating the computational results with experimental. Finally, the new model is applied to gain understanding of the experimental observed phenomena of fibres evolving to twisted ribbons. The simulations using the developed fibre model demonstrate that those twisted ribbons are formed by lateral aggregation of the fibres. The useful information obtained using the model, supports its validity. In conclusion, in this thesis the thermodynamic nature of gels is demonstrated to be able to use MD simulations and DPLs for the molecular level study of Fmoc-dipeptide nanostructures. A parameterization of the Fmoc is also developed to allow the implementation of the MD simulations for these systems. Then, standard characterization of Fmoc-dipeptide nanostructures is combined with DPLs to gain intermolecular interaction information of these systems to then use this information for an iterative model development of a fibre model, which is correlated and validated with experimental observations. This demonstrates the synergistic effect of combining computational with experimental methods to gain understanding of supramolecular nanostructures at a level which is not accessible with any other technique.

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Gröne, Bernhard. "Konzeptionelle Patterns und ihre Darstellung." Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2005/230/.

Повний текст джерела

Анотація:

Zur Beherrschung großer Systeme, insbesondere zur Weitergabe und Nutzung von Erfahrungswissen in der frühen Entwurfs- und Planungsphase, benötigt man Abstraktionen für deren Strukturen. Trennt man Software- von Systemstrukturen, kann man mit letzteren Systeme auf ausreichend hohem Abstraktionsgrad beschreiben.

Software-Patterns dienen dazu, Erfahrungswissen bezüglich programmierter Systeme strukturiert weiterzugeben. Dabei wird unterschieden zwischen Idiomen, die sich auf Lösungen mit einer bestimmten Programmiersprache beziehen, Design-Patterns, die nur einen kleinen Teil des Programms betreffen und Architektur-Patterns, deren Einfluss über einen größeren Teil oder gar das komplette Programm reicht.

Eine Untersuchung von existierenden Patterns zeigt, dass deren Konzepte nützlich zum Finden von Systemstrukturen sind. Die grafische Darstellung dieser Patterns ist dagegen oft auf Software-Strukturen eingeschränkt und ist für die Vermittlung von Erfahrungen zum Finden von Systemstrukturen meist nicht geeignet.

Daher wird die Kategorie der konzeptionellen Patterns mit einer darauf abgestimmten grafischen Darstellungsform vorgeschlagen, bei denen Problem und Lösungsvorschlag im Bereich der Systemstrukturen liegen. Sie betreffen informationelle Systeme, sind aber nicht auf Lösungen mit Software beschränkt. Die Systemstrukturen werden grafisch dargestellt, wobei dafür die Fundamental Modeling Concepts (FMC) verwendet werden, die zur Darstellung von Systemstrukturen entwickelt wurden.
Planning large and complex software systems is an important task of a system architect. It includes communicating with the customer, planning the overall system structure as well as preparing the division of labor among software engineers. What's more, a system architect benefits from other professionals' experiences concerning system architecture. By separating system from software structures, one can now describe a system by its system structure on an adequate level of abstraction.

Patterns provide a common form for the transfer of experiences. A pattern describes a widely used and proven solution to a problem that occurs in a certain context.

A study of existing architectural and design patterns shows that the concepts of many patterns carry valuable experiences concerning finding system structures. On the other hand, the graphical representations of these patterns usually focus on the software structures resulting from the solution in terms of classes and their relationships. This can be a problem if the solution doesn't imply one specific software structure but rather describes a concept which may be even independent from an implementation via software at all.

For that reason, Conceptual Patterns are introduced. A pattern can be called conceptual if both problem and solution concern system structures. Here, the functional aspects and structures of the system are relevant while code structures or even the use of software for implementation are not. To support the focus on system structures, terminology and notation of conceptual patterns should use an adequate means such as provided by the Fundamental Modeling Concepts (FMC).

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Stewart, Victor A. "A Comparison of Methods for Measuring Damage in Sucrose-Treated Medial Collateral Ligaments." Thesis, Virginia Tech, 2013. http://hdl.handle.net/10919/23110.

Повний текст джерела

Анотація:

The knee is the most complex joint in the human body. It consists of a system of muscle, bone, and ligaments that endures repetitive loading during daily and athletic activities. When this loading is excessive, damage to the knee occurs leading to a decreased quality of life.The medial collateral ligament (MCL) is one of the 4 major ligaments known to be commonly injured in the knee. The risk of injury to the knee joint increases with the elderly and individuals who experience chronic dehydration. For this reason, the focus of this study is to compare different mechanical quantitites that can be used to analyze damage to the MCL.
In this study, a novel mechanical testing protocol is used to progressively induce damage in dehydrated rat MCLs by performing tensile tests. This involves stretching the ligaments along their longitudinal axes to consecutive and increasing displacements starting at a 0.4 mm dispalcement and in increments of 0.2 mm until complete failure occurs. The load and change in length that the ligament experiences are measured at each displacement. Three different methods were evaluated to determine subfailure and damage propagation in rat MCLs: changes in tangent stiffness and chord stiffness, and changes in the load value at the 0.4 mm displacement for each load-displacement curve. The findings of this study indicate that the tangent stiffness and load at the 0.4 mm displacement provide information of the early onset of damage propagation. The decrease in chord stiffness of the ligament does not indicate damage progression in the ligament, but rather is the sign of the imminent failure of the MCL.This study provides insightful data into understanding the subfailure damage in the MCL.

Master of Science

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Brannen, Donald E. "Comparison of Selected Clinical Laboratory Tests on Adult Participants of the Fernald Medical Monitoring Program (FMMP), from the First Medical Examination from 1991 to 1994, Using the Third National Health and Nutrition Examination Survey (NHANES III) co." University of Cincinnati / OhioLINK, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ucin976116589.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Lin, Changyu. "Non-Binary Coded Modulation for FMF-Based Coherent Optical Transport Networks." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/613281.

Повний текст джерела

Анотація:

The Internet has fundamentally changed the way of modern communication. Current trends indicate that high-capacity demands are not going to be saturated anytime soon. From Shannon's theory, we know that information capacity is a logarithmic function of signal-to-noise ratio (SNR), but a linear function of the number of dimensions. Ideally, we can increase the capacity by increasing the launch power, however, due to the nonlinear characteristics of silica optical fibers that imposes a constraint on the maximum achievable optical-signal-to-noise ratio (OSNR). So there exists a nonlinear capacity limit on the standard single mode fiber (SSMF). In order to satisfy never ending capacity demands, there are several attempts to employ additional degrees of freedom in transmission system, such as few-mode fibers (FMFs), which can dramatically improve the spectral efficiency. On the other hand, for the given physical links and network equipment, an effective solution to relax the OSNR requirement is based on forward error correction (FEC), as the response to the demands of high speed reliable transmission. In this dissertation, we first discuss the model of FMF with nonlinear effects considered. Secondly, we simulate the FMF based OFDM system with various compensation and modulation schemes. Thirdly, we propose tandem-turbo-product nonbinary byte-interleaved coded modulation (BICM) for next-generation high-speed optical transmission systems. Fourthly, we study the Q factor and mutual information as threshold in BICM scheme. Lastly, an experimental study of the limits of nonlinearity compensation with digital signal processing has been conducted.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Kluth, Stephan [Verfasser], and Werner [Akademischer Betreuer] Zorn. "Quantitative modeling and analysis with FMC-QE / Stephan Kluth. Betreuer: Werner Zorn." Potsdam : Universitätsbibliothek der Universität Potsdam, 2011. http://d-nb.info/1014619114/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Damian, Fideleon II. "The road to FMFM 1: the United States Marine Corps and maneuver warfare doctrine, 1979-1989." Thesis, Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/550.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Xu, Wei. "Studies of molecular dynamics of FMOC Amino Acids using solid state deuteron nuclear magnetic resonance spectroscopy." W&M ScholarWorks, 2015. https://scholarworks.wm.edu/etd/1563899059.

Повний текст джерела

Анотація:

The purpose of devising and validating models for intramolecular motions for FMOC amino acids is to quantify side chain motion in proteins which plays an important role in understanding biological structure function relations of proteins. In this thesis, spin lattice relaxation times (Ti) of FMOC amino acids were m easured under both static and magic angle spinning (MAS) condition at variable tem peratures. Lower activation energies of the relaxation times than the normal amino acids observed indicate a less sterically crowded environment for the rotation methyl group. A three-site jump model for the methyl group w as developed to fit the Tiz and Tiq anisotropy under static condition. Under MAS, Multiple deuterated sites can be resolved and studied independently. Finally, a tem perature model for the spinning rotor w as developed to account for the tem perature gradient across the rotor. A com parison of using the single most probable tem perature and the tem perature distribution in the simulation of relaxation times concludes the difference between th ese two approaches is minimal.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Stephenson, Michael. "The utilisation of Fmoc solid phase chemistry as a novel approach to the generation of duocarmycin analogues." Thesis, University of East Anglia, 2015. https://ueaeprints.uea.ac.uk/58436/.

Повний текст джерела

Анотація:

The family is characterised by a common spirocyclopropylcyclohexadienone pharmacophore. This unusual structural motif is responsible for exceptionally efficient alkylation of adenine bases following activation through conformational changes induced by non-covalent recognition of DNA’s minor groove. This thesis describes the conception and multi-gram synthesis of a duocarmycin SA alkylation subunit suitably substituted to serve as a building block for Fmoc based solid phase synthesis and initial investigations into its application. Chapter two describes the pilot and subsequent large scale racemic synthesis of the desired duocarmycin building block, and its preparative chiral resolution by supercritical fluid chromatography. The synthesis includes a novel route to an early indole intermediate, and represents one of the shortest available strategies to access a previously reported di-Boc-protected duocarmycin structure. The large scale synthesis afforded over 8 g of the racemic Fmoc-protected building block, representing a 3 % overall yield over 13 steps. In chapter three, application of the building block to the conjugation of amino acids through solid phase synthesis is explored. This work highlights the importance of careful resin selection and the need to optimise cleavage conditions. A small library of duocarmycin analogues was generated, and subsequent assays revealed the effects of C-terminal amino acid substituents on biological activity. This work has demonstrated the potential utility of this building block for the future development of novel peptide linked antibody drug conjugates of duocarmycin SA. Finally, chapter four explores incorporation of the building block into polypyrrole structures, highlighting the potential for the direct solid phase synthesis of sequence selective bifunctional hairpin polyamides which contain the alkylation subunit of duocarmycin SA.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Pan, Jinhong. "Synthesis of Fmoc-3-(N-ethyl-3-carbazolyl)-L-alanine and its incorporation into a cyclic peptide." Link to electronic thesis, 2002. http://www.wpi.edu/Pubs/ETD/Available/etd-0814102-133523.

Повний текст джерела

Анотація:

Thesis (M.S.)--Worcester Polytechnic Institute.
Keywords: chromophore -- N-ethyl-3-carbazolylalanine -- 9-anthrylalanine -- chiral amino acids -- cyclic peptide -- solid-phase peptide synthesis -- enzymatic resolution -- cyclization. Includes bibliographical references (p. 92-95).

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Svensson, Jonny. "Implementation of an FMCW Radar Platform With High-Speed Real-Time Interface." Thesis, Linköping University, Department of Electrical Engineering, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-6163.

Повний текст джерела

Анотація:

Acreo AB has developed a radar prototype used for illustrate how the SiGe technology could be used. The radar prototype needs further development with a fast interface and a more integrated design. The beginning of the report describes the radar technique theory and the composing equations. The theoretical background is used to explain each component of the system. The report continues by specifying the target of the next radar prototype. The chosen implementation is motivated and the mode of procedure is described in detail. Test benches were used to verify correct functionality and some limits were found. The report is concluded with test results and recommendations on further enhancements.

Acreo AB har utvecklat en radarprototyp för att illustrera hur SiGe teknologi kan användas. Prototypen behöver vidareutvecklas med ett snabbt digitalt interface och en kompaktare design. Rapporten inleds med att beskriva radarteknikens funktionalitet och de utgörande ekvationerna. Den teoretiska bakgrunden används för att förklara varje komponent som systemet utgörs av. Rapporten fortsätter med att specificera målet med nästa radarprototyp. Den valda implementationen motiveras och tillvägagångssättet beskrivs detaljerat. Testuppkopplingar verifierade korrekt funktionalitet och begränsningar insågs. Rapporten avslutas med en sammanfattning av uppnådda testresultat och rekommendationer på framtida förbättringar.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Knöpfel, Andreas. "Konzepte der Beschreibung interaktiver Systeme." Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2005/289/.

Повний текст джерела

Анотація:

Interaktive System sind dynamische Systeme mit einem zumeist informationellen Kern, die über eine Benutzungsschnittstelle von einem oder mehreren Benutzern bedient werden können. Grundlage für die Benutzung interaktiver Systeme ist das Verständnis von Zweck und Funktionsweise. Allein aus Form und Gestalt der Benutzungsschnittstelle ergibt sich ein solches Verständnis nur in einfachen Fällen. Mit steigender Komplexität ist daher eine verständliche Beschreibung solcher Systeme für deren Entwicklung und Benutzung unverzichtbar.

Abhängig von ihrem Zweck variieren die Formen vorgefundener Beschreibungen in der Literatur sehr stark. Ausschlaggebend für die Verständlichkeit einer Beschreibung ist jedoch primär die ihr zugrundeliegende Begriffswelt. Zur Beschreibung allgemeiner komplexer diskreter Systeme - aufbauend auf einer getrennten Betrachtung von Aufbau-, Ablauf- und Wertestrukturen - existiert eine bewährte Begriffswelt. Eine Spezialisierung dieser Begriffs- und Vorstellungswelt, die den unterschiedlichen Betrachtungsebenen interaktiver Systeme gerecht wird und die als Grundlage beliebiger Beschreibungsansätze interaktiver Systeme dienen kann, gibt es bisher nicht.

Ziel dieser Arbeit ist die Bereitstellung einer solchen Begriffswelt zur effizienten Kommunikation der Strukturen interaktiver Systeme. Dadurch soll die Grundlage für eine sinnvolle Ergänzung bestehender Beschreibungs- und Entwicklungsansätze geschaffen werden. Prinzipien der Gestaltung von Benutzungsschnittstellen, Usability- oder Ergonomiebetrachtungen stehen nicht im Mittelpunkt der Arbeit.

Ausgehend von der informationellen Komponente einer Benutzungsschnittstelle werden drei Modellebenen abgegrenzt, die bei der Betrachtung eines interaktiven Systems zu unterscheiden sind. Jede Modellebene ist durch eine typische Begriffswelt gekennzeichnet, die ihren Ursprung in einer aufbauverwurzelten Vorstellung hat. Der durchgängige Bezug auf eine Systemvorstellung unterscheidet diesen Ansatz von dem bereits bekannten Konzept der Abgrenzung unterschiedlicher Ebenen verschiedenartiger Entwurfsentscheidungen. Die Fundamental Modeling Concepts (FMC) bilden dabei die Grundlage für die Findung und die Darstellung von Systemstrukturen.

Anhand bestehender Systembeschreibungen wird gezeigt, wie die vorgestellte Begriffswelt zur Modellfindung genutzt werden kann. Dazu wird eine repräsentative Auswahl vorgefundener Systembeschreibungen aus der einschlägigen Literatur daraufhin untersucht, in welchem Umfang durch sie die Vorstellungswelt dynamischer Systeme zum Ausdruck kommt. Defizite in der ursprünglichen Darstellung werden identifiziert. Anhand von Alternativmodellen zu den betrachteten Systemen wird der Nutzen der vorgestellten Begriffswelt und Darstellungsweise demonstriert.
Interactive systems are dynamic systems which provide services to one or more users via a user interface. Many of these systems have an information processing core. To effectively use such a system, a user needs to know about the purpose and functional concepts of the system. Only in case of a rather simple functionality, the required knowledge is likely to be obtained by mere exploration of the user interface. For complex systems, a comprehensive description is essential for effective and efficient operation, but also for system development. Especially in the context of this publication, the focus is on the diagrams that are used for communication in the development process of interactive systems.

With regard to its purpose these descriptions vary in form and notation. In any case, it is the underlying terminology which is crucial to the understandability. Established concepts and notations for the description of generic information processing systems promote a strict separation of three categories of system structures: Compositional structures, behavioral structures and value-range structures.

This publication suggests a specialization of this approach for the description of interactive systems. The definition of specific notions and semantic layers is intended to provide a complement to existing description approaches in that field that constitutes a mental framework to enhance the efficiency of communication about interactive systems. In focusing to the description aspect only, design principles, system architectures and development methods are the context but not the subject of this work.

The separation of three semantic layers provides the foundation to distinguish purpose-related, interaction-related and implementation-related models of interactive systems. Each semantic layer is characterized by a specific terminology. Referring to the idea of equivalent models, the compositional system structure varies from layer to layer and provides the framework to ask for the observable behavior and values. The strict assignment of system aspects to system components distinguishes this approach from a simple layering of design decisions in the development of interactive systems as commonly found in the relevant literature. The Fundamental Modeling Concepts (FMC) provide the foundation for the identification and representation of system structures.

A selection of system descriptions taken from the relevant literature provides the starting point to demonstrate the application of the suggested concept. Purpose, content and form of each example are analyzed with regard to the implied system structure. Weaknesses in the original representation are identified. Alternative models complement the examples to illustrate the benefit of the new approach.

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Catrinck, Tereza Cristina Pimenta Gon?alves. "Estudo da derivatiza??o de glyphosate e AMPA utilizando FMOC-CL e BSTFA para an?lises cromatogr?ficas." UFVJM, 2013. http://acervo.ufvjm.edu.br:8080/jspui/handle/1/295.

Повний текст джерела

Анотація:

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:31:00Z No. of bitstreams: 2 tereza_cristina_pmenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:31:33Z (GMT) No. of bitstreams: 2 tereza_cristina_pmenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:31:42Z (GMT) No. of bitstreams: 2 tereza_cristina_pmenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: rever nome do arquivo on 2014-12-19T12:35:15Z (GMT)
Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:36:30Z No. of bitstreams: 2 tereza_cristina_pimenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:37:00Z (GMT) No. of bitstreams: 2 tereza_cristina_pimenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:37:08Z (GMT) No. of bitstreams: 2 tereza_cristina_pimenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-19T12:37:43Z (GMT) No. of bitstreams: 2 tereza_cristina_pimenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Made available in DSpace on 2014-12-19T12:37:43Z (GMT). No. of bitstreams: 2 tereza_cristina_pimenta_goncalves_catrinck.pdf: 1238472 bytes, checksum: 651b04064f25bbcb2260de4d863373f2 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2013
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes)
Com este trabalho, objetivou-se otimizar simultaneamente metodologias para derivatiza??o de glyphosate (GLY) e ?cido aminometilfosf?nico (AMPA) para an?lises por cromatografia gasosa acoplada a espectrometria de massas (CG-EM) e cromatografia l?quida de alta efici?ncia com detector ultravioleta (CLAE-UV). Al?m disso, foram otimizadas as condi??es cromatogr?ficas por CLAE-UV para a an?lise destes compostos. O GLY foi selecionado para o estudo por ser um herbicida amplamente utilizado para o controle de pragas que, junto ao seu principal metab?lito secund?rio (AMPA) apresenta potencial risco toxicol?gico para a sa?de humana e para o meio ambiente. Devido aos seus efeitos delet?rios, alguns m?todos anal?ticos t?m sido desenvolvidos a fim de monitorar seus res?duos no meio ambiente. M?todos cromatogr?ficos t?m sido preferencialmente usados, contudo as mol?culas em estudo apresentam baixa volatilidade e aus?ncia de grupos crom?foros, sendo necess?rio adicionar etapas de derivatiza??o. Neste trabalho foram avaliadas duas metodologias para derivatiza??o das mol?culas de GLY e AMPA: com piririna e N-O(bistrimetilsilil)trifluoroacetamida (BSTFA) para an?lises por CG-EM e com cloroformiato de 9-fluorenilmetila (FMOC-Cl) para an?lises por CLAE-UV. A utiliza??o de piridina:BSTFA em propor??o 60:100, respectivamente, sem vibra??o ultrass?nica e ajuste de pH resultaram em condi??es ?timas de an?lises por CG-EM. J? para an?lises por CLAE-UV, tamp?o borato 200 mmol L-1, FMOC-Cl 500 mg L-1, homogeneiza??o por 5 minutos, an?lise imediatamente ap?s a rea??o e lavagem com ?ter diet?lico promoveram resultados satisfat?rios. Finalmente, as condi??es ?timas de an?lise por CLAE-UV foram estudas e determinou-se que a fase m?vel composta por acetonitrila/tamp?o fosfato, comprimento de onda 260 nm, gradiente com propor??o de 25:75 v/v (0-2,5 minutos), 65:35 v/v (4,5-10 minutos) e 25:75 v/v (11-12 minutos) apresentaram melhores resultados.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013.
ABSTRACT This study aimed to optimize both methodologies for derivatization of glyphosate (GLY) and aminomethylphosphonic acid (AMPA) for analysis by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography with ultraviolet detector (HPLC-UV). Moreover, the chromatographic conditions were optimized by HPLC-UV for the analysis of these compounds. The GLY was selected for the study because it is a herbicide widely used for pest control that, with its main secondary metabolite (AMPA) has potential toxicological risk to human health and the environment. Due to the deleterious effects, some analytical methods have been developed to monitor their residues on the environment. Chromatographic methods have been used preferentially, however the molecules in study have low volatility and absence of chromophoric groups being necessary to add derivatization steps. In this work were evaluated two methodologies for derivatization of GLY and AMPA molecules: pyridine and N-O(bistrimethylsilyl)trifluoroacetamide (BSTFA) for analysis by GC-MS and 9-fluorenylmethyl chloroformate (FMOC-Cl) for analysis by HPLC-UV. The use of pyridine:BSTFA in ratio 60:100, respectively, without ultrasonic vibration and adjustment pH result in better analyses conditions by GC-MS. Already for analysis by HPLC-UV, borate buffer 200 mmol L-1, FMOC-Cl 500 mg L-1, homogenization for 5 minutes, analysis immediately after reaction and washing with diethyl ether promoted satisfactory results. Finally, the optimal conditions for analysis by HPLC-UV were studied and it was determined that the mobile phase consisting of acetonitrile / phosphate buffer, wavelength 260 nm, ratio gradient 25:75 v / v (0-2.5 minutes ), 65:35 v / v (4.5-10 minutes), 25:75 v / v (11-12 minutes) showed better results.

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Mitchell, Scott Allan. "O-glycopeptide analogues of enkephalin: FMOC-amino acid glycoside synthesis, solid-phase glycopeptide synthesis and optimizations, and pharmacology." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/283920.

Повний текст джерела

Анотація:

The synthesis of a series of N-9-fluorenylmethoxycarbonyl (N-FMOC) protected amino acid glycosides is reported. These (1-2)-trans glycosides came directly from Koenigs-Knorr type glycosylations under Hanessian's silver triflate conditions, except for the synthesis of N-acetylgalactosamine FMOC amino acid in which silver perchlorate conditions were used to promote α-glycoside formation. The effect of D-amino acid aglycones was investigated under glucosylation conditions, and a yield dependence on amino protection was seen in the enantiomers of threonine. Due to this match vs. mismatch dichotomy, both O'Donnell Schiff bases and FMOC-amino aglycones were used in the subsequent glycosylation reactions. Glycosides were made using the monosaccharides xylose, mannose, glucose, galactose, N-acetylglucosamine, N-acetylgalactosamine, and disaccharides lactose [galactose-β-(1-4)-glucose], cellobiose [glucose-β-(1-4)-glucose] and melibiose [galactose-α-(1-6)-glucose]. All glycosides were converted to their respective FMOC-amino acid forms for direct use in solid-phase glycopeptide synthesis (SPGPS) using established methodology. A strategy into the synthesis of an FMOC-amino acid trisaccharide of Lewis ˣ (Leˣ) was also investigated in an effort to expand on the established glycoside methodology. Preliminary work with D-glucosamine and L-fucose is reported. Our synthetic rationale was based on retaining the peptide pharmacophore or message sequence constant as DCDCE (D-cys²ʼ⁵-enkephalin) with a serine-glycine tether, and making changes only in the environment of the amino-acid glycoside. Changes in amino acid, amino acid chirality, and in the sugar moiety itself would provide a stereochemical investigation into the requisite orientation and electronics for optimum blood-brain barrier (BBB) penetration, opiate receptor binding, and analgesia. Several glycopeptides were synthesized, and all were purified in both reduced and oxidized forms (if containing cysteine). A highly optimized glycopeptide synthetic strategy has been developed and will be presented and critiqued. Pharmacological analysis involving serum stability studies, BBB-penetration studies, GPI/MVD physicochemical studies and mu/delta-opiate receptor studies were completed on all glycopeptides. SAM-1095, the most potent of the glycopeptides synthesized, was resynthesized on a large scale, and this compound was assessed for in vivo pharmacology, along with the non-glycosylated version SAM-995. Preliminary results demonstrate an analgesic effect similar to that of the narcotic morphine. Assessment of all pharmacology will afford a platform for future SAR-based glycopeptide investigations.

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Sun, Jianhua. "Studies of Molecular Dynamics of Fmoc-Alanine-d₃ through Solid State Deuteron Nuclear Magnetic Resonance." W&M ScholarWorks, 2013. https://scholarworks.wm.edu/etd/1539626936.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Ibrahim, José-Noël. "Étude de la physiopathologie et du caractère inflammatoire de la Fièvre Méditerranéenne Familiale (FMF)." Thesis, Poitiers, 2014. http://theses.univ-poitiers.fr/62728/2014-Ibrahim-Jose-Noel-These.

Повний текст джерела

Анотація:

Afin de mieux comprendre le mécanisme physiopathologique et inflammatoire impliqué dans la fièvre Méditerranéenne familiale (FMF), nous avons étudié le profil cytokinique ex vivo monocytaire et lymphocytaire des patients FMF. Nous avons ensuite étudié l’implication potentielle dans la FMF de la protéine RAC1, qui semble jouer un rôle dans la production de l’IL-1β, d’une part, et dans la production du stress oxydant, d’autre part. Enfin, nous avons recherché une association potentielle entre les gènes IL-1β et IL-1RA et la susceptibilité et/ou la sévérité de la maladie.La première partie de l’étude a porté sur 34 patients FMF génétiquement confirmés, dont 9 ont été prélevés à deux reprises, en crise et hors crise, et 24 sujets sains. Les marqueurs inflammatoires MBL et CRP ont été dosés dans le sérum par ELISA, et les cytokines ont été quantifiées par Luminex dans le sérum et les surnageants de culture de PBMC de patients et des contrôles avec ou sans stimulation des monocytes par LPS et des lymphocytes par anti-CD3/CD28.Dans la deuxième partie de ce travail, une étude comparative des niveaux d’expression du gène RAC1 chez les patients FMF, en crise ou non, et les contrôles a été effectuée par PCR quantitative en temps réel. L’IL-1β et l’IL-6 ont été dosées par ELISA en présence ou en absence de l’inhibiteur de RAC1 dans les surnageants de culture de PBMC, avec ou sans stimulation par LPS. La quantification des marqueurs du stress oxydant a été effectuée dans le plasma ainsi que dans les surnageants de culture de PMN stimulés ou non par LPS, en présence ou en absence de l’inhibiteur de RAC1.Les génotypes au niveau des 3 polymorphismes à la position -511, -31 et +3954 d
In order to clarify the physiopathological and inflammatory mechanism underlying familial Mediterranean fever (FMF), we evaluated the ex vivo PBMC cytokine profile of FMF patients and compared it with that of controls. Then, we tried to study the implication of RAC1 protein in oxidative stress generation and IL-1β production in FMF patients. Finally, we aimed at investigating the possible association between IL- 1β and IL- 1ra genes and susceptibility and/ or severity of the disease.The first part of the study included 34 genetically confirmed FMF patients, of whom 9 were studied during attack and remission, and 24 healthy controls. Inflammatory markers CRP and MBL were measured in serum by ELISA and cytokine levels were evaluated by Luminex technology in serum and supernatants of PBMC cultures with and without 24h stimulation of monocytes by LPS and T lymphocytes by anti-CD3/CD28 beads.In the second part of this work, we compared expression levels of RAC1 gene between FMF patients, during or between crises, and controls by quantitative real-time PCR. Then, we measured spontaneous and LPS-induced production of IL-1β and IL-6 by ELISA in supernatants of PBMC cultured in the presence or absence of RAC1 inhibitor. Evaluation of oxidative stress markers was performed in plasma and in unstimlated and LPS-stimulated PMN culture supernatants in the presence or absence of RAC1 inhibitor

Стилі APA, Harvard, Vancouver, ISO та ін.

27

CADINOUCHE, KATHIA CARINA. "Aspects actuels de la fmc en medecine : resultats et commentaires d'une enquete interessant 183 medecins." Aix-Marseille 2, 1993. http://www.theses.fr/1993AIX20025.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Giribaldi, Julien. "Synthèse de peptides bioactifs inspirés des venins." Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTS124.

Повний текст джерела

Анотація:

Les extraits naturels tels que les venins animaux constituent une source importante de peptides bioactifs à visée thérapeutique. Parmi les peptides dérivés de venins utilisés en médecine, citons l’Eptifibatide, un médicament antiplaquettaire développé à partir de l’echistatin provenant d’une vipère, le Ziconotide, un puissant analgésique identifié dans le venin d’un cône ou encore l’Exenatide, un agoniste du récepteur du glucagon-like peptide 1 isolé de la salive du monstre de Gila et utilisé pour le traitement du diabète de type 2. Ces peptides riches en ponts disulfures provenant des venins présentent une structure tridimensionnelle contrainte et une stabilité plasmatique accrue comparé aux peptides linéaires. La conservation et la ressemblance des récepteurs de la proie/du prédateur avec les récepteurs humains, fait des peptides de venins une source unique de composés tête de série pour le design d’outils pharmacologiques et de composés thérapeutiques. Il est estimé que moins de 1% des peptides de venins ont été caractérisé pharmacologiquement. Ce projet a pour objectif d’explorer la pharmacologie de nouveaux peptides isolés de venins en utilisant la synthèse peptidique en phase solide basée sur la chimie Fmoc (Fmoc-SPPS) ainsi que des stratégies de repliement oxydant et régiosélectif afin de produire le peptide correctement replié responsable de l’activité biologique pour sa caractérisation ultérieure. Alors que la première partie de ce projet est dédiée à la synthèse de peptides de venins linéaires et pauvre en pont disulfures, la seconde partie sera consacrée à la synthèse de peptides riches en pont disulfures via des stratégies de repliement oxydatif et régiosélectifs. Dans un dernier chapitre, nous allons explorer les approches de protéomique intégrées à la transcriptomique pour l’identification de nouvelles séquences à partir des venins. Dans l'ensemble, ce projet permettra de mieux comprendre la pharmacologie des peptides de venins et mènera à la conception de nouveaux outils pharmacologiques et de potentiels candidats médicaments
Natural extracts such as animal venoms are an important source of bioactive peptides for therapeutic purposes. Peptides derived from venoms currently used in medicine include Eptifibatide, an antiplatelet drug developed from echistatin, a toxin isolated from a viper, Ziconotide, a potent analgesic identified in the venom of a cone snail and Exenatide , a glucagon-like peptide 1 receptor agonist isolated from the saliva of the Gila monster and used for the treatment of type 2 diabetes. These disulfide-rich venom peptides exhibit a constrained three-dimensional structure and increased plasma stability compared to linear peptides. Conservation of prey / predator receptors with human receptors makes venom peptides a unique source of lead compounds for the design of pharmacological tools and therapeutic compounds. It is estimated that less than 1% of the venom peptides have been pharmacologically characterized. Thus, this project aims to explore the pharmacology of novel venom-isolated peptides using solid phase peptide synthesis based on Fmoc chemistry (Fmoc-SPPS) as well as oxidative and regioselective folding strategies to produce the correctly folded and biologically active peptide for subsequent characterization. While the first part of this project is dedicated to the synthesis of linear and disulfide-poor venom peptides, the second part will be dedicated to the synthesis of disulfide-rich peptides via oxidative and regioselective folding strategies. Finally, we will use proteomic approaches integrated with transcriptomic data for the identification of new sequences from venoms. Overall, this project provides a better understanding of the pharmacology of venom peptides and identifies leads for the development of new pharmacological tools and potential drug candidates

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Barros, Bernardo Solé. "Cenário de mudanças e implicações na transferência do conhecimento tácito: estudo de caso FMC Technologies do Brasil." reponame:Repositório Institucional do FGV, 2013. http://hdl.handle.net/10438/13320.

Повний текст джерела

Анотація:

Submitted by Bernardo Solé Barros (bernardosole@hotmail.com) on 2015-01-22T22:11:56Z No. of bitstreams: 1 Cenário de mudanças e implicações na transferência de conhecimento tácito.versão final para download.pdf: 1378134 bytes, checksum: 4c7960434e0af2def0f74217e1cbcb4c (MD5)
Approved for entry into archive by Janete de Oliveira Feitosa (janete.feitosa@fgv.br) on 2015-02-04T19:28:02Z (GMT) No. of bitstreams: 1 Cenário de mudanças e implicações na transferência de conhecimento tácito.versão final para download.pdf: 1378134 bytes, checksum: 4c7960434e0af2def0f74217e1cbcb4c (MD5)
Approved for entry into archive by Marcia Bacha (marcia.bacha@fgv.br) on 2015-02-11T13:02:22Z (GMT) No. of bitstreams: 1 Cenário de mudanças e implicações na transferência de conhecimento tácito.versão final para download.pdf: 1378134 bytes, checksum: 4c7960434e0af2def0f74217e1cbcb4c (MD5)
Made available in DSpace on 2015-02-11T13:09:06Z (GMT). No. of bitstreams: 1 Cenário de mudanças e implicações na transferência de conhecimento tácito.versão final para download.pdf: 1378134 bytes, checksum: 4c7960434e0af2def0f74217e1cbcb4c (MD5) Previous issue date: 2013-12-19
O objetivo dessa dissertação é analisar o desafio da transferência de conhecimento no setor de tecnologia de Óleo e Gás, especificamente na empresa FMC Technologies do Brasil, e suas consequências no diferencial competitivo, ao compreender como se dá a transferência de conhecimento tácito dos especialistas para os novos colaboradores, que devido ao enorme crescimento do segmento são a maioria atualmente. O estudo de caso se dará pela observação participante do próprio autor no local de trabalho, abordagem de teoria analítica e consequente pesquisa qualitativa com colaboradores de diferentes áreas e tempos de serviço, a fim de qualificar observações, bem como entender como se estabelece este processo e fundamentalmente, como melhorá-lo e garantir sustentabilidade. Como resultado deste trabalho tivemos a validação dos especialistas como responsáveis pela transferência do conhecimento tácito dentro do ambiente e contexto capacitante criado desde a fundação da empresa CBV, o qual desdobra a estratégia através da inspiração na valorização do trabalho e sentimento de contribuição.
The main purpose of this study is to analyze the knowledge transfer challenge in the oil and gas technology industry, specifically in FMC Technologies in Brazil, and its consequences on the competitive edge by understanding how is the transfer of knowledge from the specialist to new employees , that due to the massive growth of the segment are the most current . The case study will be done by the author's own participant observation in the workplace, the analytical theory approach and consequent qualitative research with employees from different areas and service times in order to qualify observations and to understand how this process is established and fundamentally, how to improve it and ensure sustainability. As a result of this work we validate the experts as responsible for the transfer of tacit knowledge within the environment and enabling context created since the company's founding CBV, which deploy the strategy through inspiration in the recognition of work and sense of contribution.

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Osborn, Nigel John. "Characterisation of two antipeptide antibodies and the synthesis and implementation of a novel linker for use in Fmoc solid-phase peptide synthesis." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386763.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Knorr, Camila de Lellis. "Combustão iniciada por micro-ondas com radiação focalizada (FMIC) para decomposição de condimentos de origem vegetal para posterior determinação de metais por ICP OES." Universidade Federal de Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/10494.

Повний текст джерела

Анотація:

Conselho Nacional de Desenvolvimento Científico e Tecnológico
In this work the focused microwave induced combustion (FMIC) method was proposed for decomposition of spices, as Origanum vulgare L., Petrosolium sativum, Ocimum basilicum, Coriandrum sativum L. e Allium fistolosum for further Ba, Ca, Mn and Sr determination by inductively coupled plasma optical emission spectrometry (ICP OES). Origanum vulgare L. was chosen for optimization of parameters related to FMIC. For results comparison, microwave assisted wet digestion (MAWD), focused microwave assisted wet digestion (FMAWD), microwave induced combustion (MIC) and dry ashing were used to Origanum vulgare L. digestion. No statistical differences (ANOVA, confidence level 95%) were observed between the results obtained by MAWD, FMAWD, MIC, FMIC and dry ashing. Agreement for certified reference materials of apple leaves (NIST 1515) and peach leaves (NIS1547) after FMIC digestion ranged from 97 to 103%. Values of residual carbon content in digests obtained after MAWD, FMAWD, FMIC and MIC procedures were 0.93, 2.73, 0.17 e 0.04 mg de C/100 mg, respectively. The main advantages related to the use of FMIC are: the possibility to use diluted acids as absorbing solution, sample masses up to 1 g can be digested allowing lower limits of detection and digestion time is lower in comparison to the other methods evaluated in this work.
No presente trabalho é proposta a combustão iniciada por micro-ondas com radiação focalizada (FMIC) para a decomposição de condimentos de origem vegetal, para posterior determinação de Ba, Ca, Mn e Sr por espectrometria de emissão óptica com plasma indutivamente acoplado (ICP OES). Origanum vulgare L. foi utilizado para a otimização dos parâmetros relacionados à decomposição por FMIC. Para comparação dos resultados, a amostra de Origanum vulgare L. foi também decomposta por via úmida assistida por radiação micro-ondas em sistema fechado (MAWD), por via úmida assistida por micro-ondas com radiação focalizada (FMAWD), por combustão iniciada por micro-ondas em sistema fechado (MIC) e por decomposição por via seca em mufla. Os resultados obtidos por MAWD, FMAWD, MIC, FMIC e por decomposição por via seca com mufla não apresentaram diferença estatística significativa (ANOVA, nível de confiança 95%) entre si. Para avaliar a exatidão do método proposto, materiais de referência certificados de folhas de macieira (NIST 1515) e folhas de pessegueiro (NIST 1547) foram utilizados e foram obtidas concordâncias entre 97 e 103% para os elementos avaliados. Os teores de carbono residual nos digeridos obtidos por MAWD, FMAWD, FMIC e MIC foram de 0,93, 2,73, 0,17 e 0,04 mg C/100 mg de amostra, respectivamente. Dentre as vantagens do uso do método de decomposição por FMIC destacam-se: a possibilidade do uso de ácido diluído como solução absorvedora, massas de amostra de até 1 g podem ser decompostas, permitindo a obtenção de menores limites de detecção e a decomposição é feita em menor tempo quando comparada com os demais métodos avaliados.

Стилі APA, Harvard, Vancouver, ISO та ін.

32

RIBEIRO, LEONARDO DE SOUSA. "STRATEGIC FIT ANALYSIS OF A TECHNOLOGY COMPANY IN THE BRAZILIAN OFFSHORE OIL AND GAS MARKET: CASE STUDY OF FMC TECHNOLOGIES." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2006. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=8903@1.

Повний текст джерела

Анотація:

O mercado brasileiro de produção de petróleo e gás offshore tem se desenvolvido significativamente nos últimos anos, alavancado pela alta mundial do preço do petróleo e pelo incentivo do Governo ao alcance da auto-suficiência na produção de petróleo. As maiores e mais promissoras reservas brasileiras de petróleo e gás estão localizadas no mar, em águas cada vez mais profundas. Novas tecnologias são necessárias para viabilizar a extração do petróleo destes campos. Oito anos após a abertura do setor de produção e exploração, novas operadoras começam a fazer grandes investimentos no país. Neste ambiente, as empresas fornecedoras de produtos e serviços têm grandes oportunidades de crescer seus negócios, porém também têm grandes desafios relacionados à capacitação do corpo técnico e ampliação da capacidade produtiva. Este trabalho tem como objetivo auxiliar a FMC Technologies, umas das empresas fornecedoras de produtos de tecnologia, na avaliação da adequação de sua estratégia no Brasil e propor mudanças, com o objetivo de criar vantagens competitivas sustentáveis de longo prazo. A investigação foi desenvolvida usando a metodologia de estudo de caso. Coletados por meio de investigação documental/ telematizada, questionário predominantemente estruturado e entrevistas. Os dados da pesquisa foram tratados com métodos quantitativos e qualitativos. A interpretação e a análise dos dados foram realizadas com base no referencial teórico, fundamentado em revisão de literatura pertinente à área de estudo, com auxílio do modelo genérico integrativo de Macedo- Soares (2001). Os resultados evidenciaram algumas inconsistências entre os objetivos estratégicos explicitados pela empresa, os fatores organizacionais, os fatores marcoambientais econômicos e os atores estratégicos. Ao mesmo tempo, trouxeram subsídios para identificar quais mudanças seriam necessárias para superar estas inconsistências.
The Brazilian offshore oil and gas market increased significantly in the last years, stimulated by the oil high price and by the government incentive for the country selfsufficiency. The bigger and more promising Brazilian oil and gas reserves were located in deeper waters. New technologies and products are demanded to make feasible oil extraction. After eight years of exploration and production sectors opening, new operators start to make wide investments. Considering this environment, the product and service suppliers have great opportunities to develop their business, but have also big challenges related to the qualification of technical personnel and production expansion. The objective of this study is to support FMC Technologies do Brasil, a technological product supplier, in the evaluation of the adequacy of its strategic in Brazil. It also aims to recommend changes that will improve the fit of the company`s actions with the objectives, creating long term competitive advantages. The investigation was carried out by means of a case study methodology. Data collected through documental/ telematic investigations, mainly structured questionnaires and interviews, were threated with qualitative and quantitative methods. The data interpretation and analysis were conducted based on the theoretical references from the literature review, with the support of the systemic and integrative model developed by Macedo-Soares (2001). The results highlight some inconsistencies between the firm s strategy objectives, its organizational factors, the macro-environmental factors and the strategic actors. In addition, the changes required to overcome those inconsistencies were identified.

Стилі APA, Harvard, Vancouver, ISO та ін.

33

連秀屘 та Hsiu-man Lien. "葡萄糖胺-FMOC 衍生化反應之高效能液相層析法分析方法開發". 碩士, 朝陽科技大學, 1992. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22092CYUT5500002%22.&searchmode=basic.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Eido, Souheir. "Contrôle de la mobilité dans un réseau d'opérateur convergé fixe-mobile." Thesis, Ecole nationale supérieure Mines-Télécom Atlantique Bretagne Pays de la Loire, 2017. http://www.theses.fr/2017IMTA0025/document.

Повний текст джерела

Анотація:

Les réseaux fixes et mobiles font face à une croissance dramatique du trafic de données, qui est principalement due à la distribution de contenus vidéo. Les opérateurs Télécoms envisagent donc de décentraliser la distribution de contenus dans les futures architectures convergées fixe-mobile (FMC). Cette décentralisation, conjointement au déploiement d'un cœur de réseau mobile distribué, sera un élément majeur des futurs réseaux 5G. L'approche SIPTO définie par 3GPP permet déjà le délestage sur le réseau fixe du trafic mobile, et pourra donc être utilisée en 5G. SIPTO s'appuie sur la distribution des passerelles de données (PGW) qui permet ainsi de décharger le cœur du réseau mobile actuel. Cependant, dans certains cas de mobilité des usagers, SIPTO ne supporte pas la continuité de session, quand il est nécessaire de changer de PGW, donc de modifier l'adresse IP du terminal. Cette thèse commence par quantifier le gain apporté par le délestage du trafic mobile en termes de capacité requise pour différentes portions du réseau. Un état de l'art des différentes solutions de délestage du trafic de données mobiles est fourni, démontrant qu'aucune des solutions existantes ne supporte la continuité de service pour les sessions de longue durée. C'est pourquoi, cette thèse propose des solutions pour supporter une mobilité transparente ; ces solutions s'appuient à la fois sur SIPTO et sur le protocole MultiPath TCP (MPTCP). Les protocoles du 3GPP sont inchangés car la continuité est maintenue par les extrémités. Enfin, ces solutions sont appliquées aux différentes implémentations d'architectures FMC envisagées à ce jour
Fixed and mobile networks are currently experiencing a dramatic growth in terms of data traffic, mainly driven by video content distribution. Telecoms operators are thus considering de-centralizing content distribution architecture for future Fixed and Mobile Converged (FMC) network architectures. This decentralization, together with a distributed mobile EPC, would be used for future 5G networks. Mobile data offloading, in particular SIPTO approaches, already represent a good implementation model for 5G network as it allows the use of distributed IP edges to offload Selected IP traffic off the currently centralized mobile core network. However, in some cases, SIPTO does not support session continuity during users' mobility. This is due to the fact that user's mobility may imply packet gateway (PGW) relocation and thus a modification of the UE's IP address.This PhD thesis first quantifies the gain, in terms of bandwidth demands on various network portions, brought by the generalized use of mobile traffic offloading. A state of art of existing mobile data offloading solutions is presented, showing that none of the existing solutions solve the problem of session continuity for long-lived sessions. This is why, in the context of future FMC mobile network architectures, the PhD thesis proposes solutions to provide seamless mobility for users relying on SIPTO with the help of Multipath TCP (MPTCP). 3GPP standards are not modified, as session continuity is ensured by end-points. Lastly, the proposed solutions are mapped on different architecture options considered for future FMC networks

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Silva, Maria João da Costa Soares da. "Clinical and molecular characterization of feline mammary carcinomas overexpressing HER2 proto-oncogene (FMC-HER2+) : new strategies for effective diagnostic and cancer therapy." Doctoral thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2016. http://hdl.handle.net/10400.5/12008.

Повний текст джерела

Анотація:

Tese de Doutoramento em Ciências Veterinárias na Especialidade de Ciências Biológicas e Biomédicas
mariajosoares@gmail.com
Considering the scarce data available in feline mammary carcinoma (FMC) and despite its importance in veterinary clinical practice, this thesis emerges in order to increase the knowledge of this tumor type, especially the FMC-HER2 positive. In the first two studies, the protocols for detection and quantification of the fHER2 and Ki-67 biomarkers were optimized and validated. These studies demonstrated that, in cats, the incidence of fHER2 overexpression were similar to women (about 30%), although no gene amplification was detected. It was also demonstrated that high levels of Ki-67 index were associated with a worse prognosis. Using a panel of protein biomarkers, the FMC were divided into six different groups that demonstrated prognostic value, similarly to what is described in women. In fact, cats with triple negative basal-like or HER2-positive subtypes were associated with shorter overall survival, contrasting with cats presenting luminal A tumors. Moreover, these studies also indicated that luminal B and triple negative basal-like subtypes are the most common in cats. When the metastatic lesions were evaluated, a marked loss of receptor expression was found, which was associated with an increase of the triple negative basal-like subtype, highlighting the importance of immunophenotyping all lesions (primary and metastatic) in cats. Considering these results, the development of diagnostic methodologies that allows the continuous follow-up of the patients would be very useful. Therefore, the last study presented in this thesis evaluates the fHER2 serum levels in cats with FMC using two different immunoassays (ELISA and dot blot). The serum levels of fHER2 were significantly associated with the fHER2 in tissue samples of FMC (assessed by IHC). This is consistent to what is described for humans and suggests that serum quantification could be an important tool for monitoring cats after the surgery. In sum, the results presented herein provide new diagnostic and prognostic tools for veterinary oncology. Considering the high prevalence and similarities with the human counterpart, cat can also represent a potential animal model for the study of luminal B and triple negative subtypes. Considering fHER2-positive FMC more studies are required in order to determine the aetiology of the protein overexpression.
RESUMO - Os tumores mamários felinos (TMF) são umas das neoplasias mais comuns em Oncologia Felina, com uma incidência que pode atingir os 40%, pelo que assumem um papel relevante na prática clínica veterinária. Estes tumores apresentam habitualmente uma etiologia maligna (carcinomas) e um comportamento agressivo, estando associados a um prognóstico reservado. Atualmente, existem poucas opções terapêuticas que permitam aumentar a qualidade e a esperança média de vida dos animais afectados por esta neoplasia. Assim, estudos que permitam uma melhor caracterização dos tumores, identificando potenciais biomarcadores que possam ser utilizados como factores de prognóstico ou preditivos, são fundamentais para o desenvolvimento da Medicina Felina. Por outro lado, os carcinomas mamários que ocorrem espontaneamente nos animais de companhia têm sido sugeridos como potenciais modelos biológicos para o estudo do Cancro da Mama, com vantagens, comparativamente aos animais de laboratório que são atualmente utilizados. De facto, nesses animais, os tumores são quimicamente induzidos ou xenotransplantados, pelo que são considerados modelos mais artificiais. Deste modo, esta tese de doutoramento surge com o objetivo de aumentar o conhecimento sobre os tumores mamários felinos, com especial interesse no recetor transmembranar para o fator de crescimento epidérmico de tipo 2 (HER2), quer numa perspetiva clínica, de forma a melhorar a qualidade de vida destes animais de companhia, abrindo portas a novos meios de diagnóstico e potenciais novos alvos terapêuticos, quer no sentido de investigar a viabilidade de a Gata ser um bom modelo animal para o estudo do Cancro da Mama na Mulher. Relativamente à Mulher, a investigação oncológica desenvolveu grandes esforços para encontrar biomarcadores que permitam otimizar o diagnóstico e o tratamento do cancro da mama. Entre estes, encontra-se a proteína HER2, uma oncoproteína que pode estar sobreexpressa em vários tumores da espécie humana (mama, pâncreas, cólon, próstata, bexiga, entre outros) e que lhes confere elevada agressividade e prognóstico reservado. No Cancro da Mama, estima-se que entre 10 a 40% dos tumores apresentem amplificação do gene HER2, o que se traduz na sobreexpressão da proteína. Estas alterações são rotineiramente detetadas através de duas técnicas moleculares, a hibridação in situ (ISH) e a imunohistoquímica (IHC), respetivamente. A avaliação do status da proteína HER2 é importante, não só pelo seu valor de prognóstico, mas também como fator preditivo, já que pacientes com sobreexpressão desta proteína são elegíveis para tratamento com terapêuticas específicas dirigidas contra o HER2, tais como os anticorpos anti-HER2 (sendo o trastuzumab o mais conhecido), o que veio aumentar consideravelmente a sobrevida destas doentes. Contrastando com a medicina humana, a literatura disponível em medicina veterinária apresenta ainda escassos estudos sobre a importância da proteína HER2 nos TMF, ou sobre os novos sistemas de classificação que subdividem os tumores mamários conforme o seu perfil imunofenotípico.(...)

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Aldea, Tomé Ana Isabel. "Bases genètiques de la febre mediterrània familiar a la població espanyola, dinàmica genòmica i història natural de les mutacions al locus "MEFV"." Doctoral thesis, Universitat de Barcelona, 2004. http://hdl.handle.net/10803/858.

Повний текст джерела

Анотація:

La febre mediterrània familiar (FMF) afecta unes 10.000 i 100.000 persones d'origen principalment àrab, armeni, turc i jueu i és caracteritza per la recurrència d'episodis autolimitats de febre i inflamació de seroses, sense una causa infecciosa aparent. A la població espanyola, els pacients de FMF descrits són casos aïllats i el recull més extens és el de pacients xuetes de Mallorca. La recent clonació del gen MEFV, al 1997, va constituir un punt d'inflexió en l'estudi de la FMF en els més diversos aspectes de la malaltia i va crear grans expectatives en els camps del diagnòstic, terapèutic i preventiu. A més, l'estudi en poblacions no ancestrals era un camp totalment inexplorat, començant pel fet que es considerava que la FMF no les afectava.
La hipòtesi de treball d'aquesta tesi s'ha basat en què l'estudi de les mutacions del MEFV en els pacients de FMF espanyols, així com la detecció de la possible intervenció de factors modificadors de la malaltia, permetria establir la relació causal de les mutacions al gen MEFV i la FMF i establir-ne les bases genètiques. A més, l'estudi de la dinàmica genòmica del locus MEFV permetria estudiar la història natural de les mutacions més freqüents a la vessant occidental de la Mediterrània.
Les conclusions de l'estudi són, en quant a les bases genètiques de la FMF a la població espanyola, que només el 53% dels pacients de FMF espanyols tenen una o dues mutacions en el gen MEFV, essent l'espectre de mutacions força heterogeni, mentre que el 47% dels pacients espanyols diagnosticats clínicament de FMF no presenta cap mutació al MEFV ni en cap dels gens coneguts responsables d'altres SHFP. Existeix una alta freqüència de pacients heterocigots qüestiona el patró d'herència de la FMF i suggereix l'existència d'almenys un altre locus implicat en la malaltia. D'altra banda, els pacients espanyols diagnosticats clínicament de FMF probable sense mutacions al gen MEFVpodrien manifestar un fenotip de FMF inusual o bé una nova síndrome de febre periòdica, altrament anomenada, FMF-like.
Els pacients de FMF a la població espanyola no pertanyen a cap ètnia relacionada amb les de les poblacions anomenades ancestrals, per la qual cosa l'origen ètnic no s'hauria de considerar un criteri d'exclusió en el diagnòstic clínic de FMF a la població espanyola. Clínicament, els pacients espanyols diagnosticats de FMF constitueixen un grup heterogeni: un subgrup manifesta la simptomatologia clínica típica de la malaltia, on ni l'amiloïdosi ni l'ELE ni l'historial familiar són manifestacions clíniques freqüents, mentre que l'altre subgrup manifesta episodis lleugerament més llargs i amb una major freqüència de limfadenopaties i mialgia. Es conclou que és necessari afegir l'anàlisi de la totalitat dels exons del MEFV en el diagnòstic de la FMF a la població espanyola.
A la població general espanyola s'observa una diversitat d'haplotips del MEFV força limitada causada per l'alt LD dels subhaplotips de cada bloc. L'equilibri de lligament entre els haplotips majoritaris del gen MEFV i l'alt LD de cada subhaplotip suggereix l'existència d'un punt calent de recombinació a l'intró 2, que constituiria un mecanisme plausible de generació dels haplotips majoritaris. Es proposa, per primera vegada, una nomenclatura sistematitzada dels haplotips de SNPs intragènics al gen MEFV, basada en criteris genètics i independentment del grup ètnic on s'han estat descrits. A més, es descriuen Tag-SNPs, per tal d'optimitzar la recerca de futurs estudis.
L'estudi dels haplotips associats a mutacions al MEFV en pacients de FMF revela la proximitat genètica de la població espanyola amb altres poblacions no ancestrals com la italiana o la francesa i una clara distància amb les poblacions ancestrals i estaria d'acord amb l'origen jueu sefardita dels xuetes.

Стилі APA, Harvard, Vancouver, ISO та ін.

37

SANDRINI, VITOR DE UZEDA. "ANALYSIS OF STRATEGIC ALLIANCES, MERGERS AND ACQUISITIONS OF COMPANIES THAT COMPETE GLOBALLY IN THE OIL AND GAS INDUSTRY: A CASE STUDY OF FMC TECHNOLOGIES." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2014. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=24482@1.

Повний текст джерела

Анотація:

A indústria brasileira de óleo e gás é, atualmente, uma das mais atrativas do mundo. Investidores, fabricantes de equipamentos e companhias de petróleo procuram a todo momento formas de entrar neste mercado para sustentar sua competitividade. Devido à complexidade e aos elevados investimentos necessáriosà atuação neste setor, as alianças estratégicas, fusões e aquisições tornam-se indispensáveis para o aproveitamento dessas oportunidades. Desta maneira, o objetivo deste trabalho consiste em analisar as alianças estratégicas, fusões e aquisições da empresa FMC Technologies com seus atores-chave no setor de óleo e gás. A investigação foi conduzida mediante o emprego da metodologia de estudo de caso, com triangulação de métodos. Assim, os dados da pesquisa foram coletados por vários meios, a saber: investigação documental/telematizada e levantamentos de percepções, com o auxílio de questionário predominantemente estruturado e de entrevistas respectivamente. Tais elementos foram tratados com base no referencial teórico, fundamentado na revisão da literatura pertinente à área de estudo, com o auxílio da ferramenta analítica Global SNA Framework de Macedo-Soares (2011). Os resultados da pesquisa evidenciaram uma adequação entre os objetivos estratégicos explicitados pela empresa e as alianças e F&A realizadas com parceiros, proporcionando um aumento das oportunidades e uma redução das ameaças no nível da indústria. Como recomendação resultante da pesquisa, sugere-se a realização de novos trabalhos enfocando os principais concorrentes da FMC Technologies os quais também competem globalmente por meio de alianças globais no setor de óleo e gás.
The Brazilian oil and gas industry is currently one of the most attractive worldwide. Investors, equipment manufacturers and oil companies continuously seek ways to enter in this market to sustain its competitiveness. Due to the complexity and high investments required to operate in this sector, strategic alliances, mergers and acquisitions become mandatory to benefit from such opportunities. Thus, the aim of this thesisis to analyze strategic alliances, mergers and acquisitions of FMC Technologies with their key players in the oil and gas sector. The investigation was conducted using a case study methodology, with triangulation of methods. The survey data were collected by different ways: analysis of documents, telematic and perception research, using surveys and predominantly structured interviews. The data treatment was based on the theoretical framework, pursuant to a review of the relevant literature, with the support of the analytical tool GlobalSNA Framework Macedo-Soares (2011). The study results showed a match between the strategic objectives for the company and explained the alliances and M&A transactions with partners, providing increased opportunities and reducing threats in the industry level. As a result from the research, it is recommendedto conduct new studies focused on the main competitors of FMC Technologies, whose international operations are also based on global alliances in the oil and gas sector.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Arlt, Dorit Hella. "Untersuchungen zur Aktivierung des RET-Tyrosinkinase-Rezeptors mit seltenen MEN-2A-FMTC-assoziierten Mutationen in der extrazellulären Domäne in der Mäusefibroblasten-Zelllinie NIH3T3 und der humanen neuroektodermalen Tumorzelllinie SK-N-MC." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=965216233.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Aguilar, Malagón Victor. "LA HISTORIA TAMBIÉN JUEGA. HISTORIA DEL FUTBOL EN MÉXICO Y SU PAPEL EN LA IDENTIDAD NACIONAL A PARTIR DE TRES CATEGORÍAS ANALÍTICAS: PRÁCTICA, DISCURSO Y REPRESENTACIÓN." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2020. http://hdl.handle.net/20.500.11799/110370.

Повний текст джерела

Анотація:

Actualmente, el deporte representa un símbolo de identidad social, sin embargo; los libros de historia difícilmente mencionan dicha práctica. El futbol, en específico, se ha convertido un generador de pasiones y sentimientos, en ocasiones difíciles de comprender pero que vale la pena historiar. De esta forma, el presente trabajo de tesis parte del siguiente cuestionamiento: ¿A qué se debe que el futbol haya sido el deporte más popular en México durante el siglo XX y aún continúe siendo en el siglo XXI? Y ¿Por qué el futbol alimenta la identidad nacional mexicana? Para una correcta redacción y una mejor comprensión de la temática seleccionada, se optó por echar mano de la metodología aportada por Roger Chartier, de la cual se toman tres categorías analíticas: practica, discurso y representación. De esta manera, el trabajo se divide en tres capítulos, uno por cada categoría analítica, con el objetivo de abordar el tema a partir de diversos fenómenos sociales, los cuales mostrarán la respuesta a las preguntas principales. En un principio se aborda la historia de la práctica del futbol en México, desde su aparición hasta su consolidación. A continuación, se analiza el discurso que envuelve al futbol, mencionando a las instituciones de mayor importancia y el papel que los medios de comunicación han jugado en la evolución de este deporte. Finalmente, se estudian las representaciones, que nos hablan, mediante imágenes, sobre la identidad nacional en el futbol. Con un sustento metodológico y mediante la investigación histórica, este trabajo de investigación pretende mostrar la importancia del futbol, proveerle un correcto estudio, y construir un conocimiento acorde a dicha significación, para así, permitirle encontrar un espacio de reconocimiento y crítica.

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Mahl, Deise Luiza. "SUSCEPTIBILIDADE in vitro DE ISOLADOS DE Pythium insidiosum FRENTE A AMINOGLICOSÍDEOS E TIGECICLINA." Universidade Federal de Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/8960.

Повний текст джерела

Анотація:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Pythiosis is a chronic disease that affects humans, other mammals and birds. It is caused by the aquatic oomycete Pythium insidiosum. Pythiosis progresses rapidly and can become life threatening if not treated in the early stages. The absence of ergosterol in the cell wall of this oomycete prevents the treatment of pythiosis with antifungal therapy because most antifungal drugs act by inhibiting the synthesis of ergosterol. Members of the genus Pythium are known to be susceptible to some antimicrobial of the tetracycline, macrolide, aminoglycoside and chloramphenicol classes. This study aimed to evaluate the in vitro susceptibility of isolates of P. insidiosum to the aminoglycosides gentamicin, neomycin, paromomycin and streptomycin and to the minocycline derivative tigecycline. The susceptibility tests were carried out with 24 P. insidiosum isolates using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute (CLSI) document M38-A2. The minimum inhibitory concentration (MIC) values for gentamicin, neomycin, paromomycin and streptomycin ranged from 32 to 64 mg/L, and the minimal fungicidal concentration (MFC) values ranged from 32 to 128 mg/L. Tigecycline had a good inhibitory activity, with values of mic MIC (0.25-2 mg/L) and MFC (1-8 mg/L) values. The observed in vitro susceptibility to tigecycline makes this drug a good option for future in vivo assays investigating options of treatment of pythiosis.
Pitiose é uma doença crônica que afeta humanos, outros mamíferos e pássaros. O seu agente etiológico é um oomiceto aquático denominado Pythium insidiosum. A Pitiose evolui rapidamente e pode se tornar fatal se não tratada nos primeiros estágios. A ausência de ergosterol na parede da célula deste oomiceto impede o tratamento de pitiose com terapia antifúngica, pois a maioria das drogas antifúngicas atua sobre a síntese de ergosterol. Os membros do gênero Pythium são conhecidos por serem susceptíveis a alguns antimicrobianos do grupo das tetraciclinas, macrolídeos, aminoglicosídeos e cloranfenicol. Este estudo teve como objetivo avaliar a susceptibilidade in vitro de isolados de P. insidiosum frente aos aminoglicosídeos gentamicina, neomicina, paromomicina e estreptomicina e ao derivado da minociclina denominado de tigeciclina. Os testes de susceptibilidade foram realizados com 24 isolados de P. insidiosum, utilizando o método de microdiluição em caldo, de acordo com o documento M38- A2 do Clinical and Laboratory Standards Institute (CLSI). Os valores de Concentração Inibitória Mínima (CIM) de gentamicina, neomicina, paromomicina e estreptomicina variaram de 32 a 64 mg/L, e os valores de concentração fungicida mínima (CFM) variaram de 32 a 128 mg/l. Tigeciclina apresentou boa atividade inibitória, com valores de CIM (0,25-2 mg/L) e CFM (1-8 mg/L). A susceptibilidade à tigeciclina observada in vitro faz deste fármaco uma boa opção em futuros ensaios e in vivo, investigando o tratamento da pitiose.

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Moussallem, Márcia. "Terceiro setor: um ator estratégico para o desenvolvimento humano Fundação Dona Mindoca Rennó Moreira- Escola Técnica de Eletrônica Francisco Moreira da Costa-FMC-ETE: Santa Rita do Sapucaí- MG-2013." Pontifícia Universidade Católica de São Paulo, 2014. https://tede2.pucsp.br/handle/handle/17670.

Повний текст джерела

Анотація:

Made available in DSpace on 2016-04-29T14:16:30Z (GMT). No. of bitstreams: 1 Marcia Moussallem.pdf: 44289054 bytes, checksum: 56a9edd6f0a67e07a56d4c2931d44ff5 (MD5) Previous issue date: 2014-03-28
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
This thesis has as its theme the role of a third sector organization in human development in a municipality. Dona Mindoca Rennó Moreira Foundation - Technical School of Electronics Francisco Moreira da Costa (FMC ETE) was chosen as a research study case. This organization is located in Santa Rita do Sapucaí, Minas Gerais State, which is a third sector organization with innovative actions in the field of technological education. Was formulated as a hypothesis that the FMC-ETE while a third sector organization innovated and transformed, through their actions in the educational field, the economic, social, political and cultural profile of the municipality of Santa Rita do Sapucaí, turning into a strategic actor in promoting human development of this municipality. The general objective aimed to analyze the innovative actions of the Foundation since its beginning in 1959. The theoretical references comprise the concepts of third sector and human development based on classical authors of contemporary areas of study in social sciences and social service. It was adopted an exploratory approach methodology of quantitative and qualitative research for the case study of FMC-ETE. Indicators of human development were analyzed and conducted interviews with 25 residents of the city including: alumni, entrepreneurs, directors and employees of FMC -ETE, directors and teachers of INATEL and FAI. The research main results were: actions and innovative initiatives for development are not exclusive of state and private enterprises as the classical theories use to state, but also can be the result of the initiatives of third sector organizations. The strong investment in education made by FMC-ETE provided the human development of the city, collaborating for the formation of a culture of active citizenship and social participation
A presente tese tem como tema a atuação de uma organização do terceiro setor no desenvolvimento humano de um município. Como objeto de pesquisa foi delimitado o estudo da Fundação Dona Mindoca Rennó Moreira - Escola Técnica de Eletrônica Francisco Moreira da Costa-FMC-ETE localizada em Santa Rita do Sapucaí- Minas Gerais, sendo esta uma organização do terceiro setor com ações inovadoras no campo da educação tecnológica. Formulou-se como hipótese que a FMC-ETE enquanto uma organização do terceiro setor inovou e transformou por meio de suas ações no campo educacional o perfil econômico, social, político e cultural do município de Santa Rita do Sapucaí, se constituindo em um ator estratégico na promoção do desenvolvimento humano deste município. O objetivo geral visou analisar as ações inovadoras da Fundação desde a sua criação em 1959. As referências teóricas compreenderam os conceitos de terceiro setor e desenvolvimento humano fundamentados em autores clássicos e contemporâneos das áreas das ciências sociais e do serviço social. Adotou-se como metodologia uma abordagem exploratória de pesquisa quantiqualitativa por meio do estudo de caso da FMC-ETE. Foram analisados os indicadores de desenvolvimento humano e realizadas entrevistas com 25 moradores da cidade entre eles: exalunos, empresários, diretores e funcionários da FMC-ETE, diretores e professores do INATEL e da FAI. Os principais resultados evidenciados na pesquisa foram: as ações e iniciativas inovadoras voltadas para o desenvolvimento não são de exclusividade do Estado e das empresas como afirmam as teorias clássicas sobre o tema, mas também das iniciativas das organizações do terceiro setor; o forte investimento em educação da FMC-ETE propiciou o desenvolvimento humano do município, contribuindo para a formação de uma cultura de cidadania de ativa e de participação social

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Lundberg, Jonas. "MPEG-4 Facial Feature Point Editor." Thesis, Linköping University, Department of Electrical Engineering, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1401.

Повний текст джерела

Анотація:

The use of computer animated interactive faces in film, TV, games is ever growing, with new application areas emerging also on the Internet and mobile environments. Morph targets are one of the most popular methods to animate the face. Up until now 3D artists had to design each morph target defined by the MPEG-4 standard by hand. This is a very monotonous and tedious task. With the newly developed method of Facial Motion Cloning [11]the heavy work is relieved from the artists. From an already animated face model the morph targets can now be copied onto a new static face model.

For the Facial Motion Cloning process there must be a subset of the feature points specified by the MPEG-4 standard defined. The purpose of this is to correlate the facial features of the two faces. The goal of this project is to develop a graphical editor in which the artists can define the feature points for a face model. The feature points will be saved in a file format that can be used in a Facial Motion Cloning software.

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Lawton-Misra, Nita. "Crisis leadership at South African universities: An exploration of the effectiveness of the strategies and responses of university leadership teams to the #FeesMustFall (#FMF) protests at South African universities in 2015 and 2016." University of the Western Cape, 2019. http://hdl.handle.net/11394/7096.

Повний текст джерела

Анотація:

Doctor Educationis
The #FeesMustFall (#FMF) movement which began in 2015 and continued in 2016 was initially a call for free tuition, but soon grew to include substantial academic demands, quickly spiralling into violence and destruction of property. This required university leaders to step into roles for which they were largely untrained and inexperienced –– even for those who were once among the ranks of the protesting students. Neither the operational systems nor the personnel had ever conceived of or anticipated such an unprecedented revolt, and the leadership had to summon all their intuition and acumen to navigate, deciding whether to merely defend their institutions or to concede to students’ demands. Did they manage the moment or lead it, and did they steel their institutions against similar future confrontations? This study used an interpretative phenomenological analysis (IPA) approach to answer the primary research question, and to understand the lived experiences of the participants, which aligned with the interpretive paradigm. Leadership theories and chaos and complexity theories were employed and provided the lens through which the data was collected and analysed. Semi-structured interviews were used as data collection methods with 29 participants. University leaders and staff who did not belong to the leadership band of universities, from six universities participated in the study. The findings revealed that South African university leaders are not adequately trained to lead during crises, and that leadership-enhancement programmes need to be developed to include this component in the training of future leaders for the higher education sector. It further revealed that the Department of Higher Education and Training should take a proactive role in training and supporting university leaders, as well as developing a national communication strategy. This research makes a contribution towards crisis leadership in the South African higher education sector by providing insights for both university leaders as well as the Ministry of Higher Education and Training, as well as proposing a model of crisis leadership.

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Hastenreiter, Roberto Soares da Cruz. "Das palavras aos quanta: analogia como elemento do pensamento e ferramenta didática em aulas de física quântica na educação básica." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/81/81131/tde-17122015-113723/.

Повний текст джерела

Анотація:

O presente trabalho trata do uso de analogias em atividades didáticas voltadas às aulas de ciências e, mais especificamente, do uso de analogias em aulas sobre temas de física quântica para alunos do ensino médio. Partimos, então, da aparente contradição que resulta da tentativa de abordar conceitos da física quântica, conceitos estes abstratos e sem relação com a realidade sensível, por meio de um mecanismo representativo que é baseado na comparação entre dois domínios do conhecimento, no qual um deles pertence ao repertório do conhecido (familiar) e o outro diz respeito ao que se deseja conhecer. Nossa principal contribuição, ligada à nossa proposição inicial, se dá na medida em que propomos inicialmente reflexões a respeito do conceito de analogia em três dimensões: a primeira, associada à perspectiva da psicologia cognitiva, na qual a analogia se constitui como elemento central do pensamento e assim da formação dos conceitos em toda a história do sujeito; a segunda, relacionada à epistemologia da ciência, especificamente sob a perspectiva de Henri Poincaré, a partir da qual se apontam basicamente três categorias de analogias (figurativas, mecânicas e matemáticas); a terceira, ligada às atividades didáticas que consideram a analogia como ferramenta de aprendizagem, e que busca, a partir de esforços coletivos presentes na literatura especializada em ensino de ciências, sistematizar o seu uso. A partir de uma ampla leitura das três dimensões supracitadas, propomos uma síntese que nos permita um olhar específico para nosso objeto de pesquisa. A fim de analisar o potencial de nossa síntese teórica, realizamos um ensaio empírico que consistiu na elaboração de episódios criados a partir de aulas de temas de física quântica ministradas a alunos do ensino médio (modelos atômicos, efeito fotoelétrico, dualidade onda-partícula, interpretações da Mecânica Quântica). A metodologia usada na interpretação dos episódios pressupõe que analogias em atividades didáticas podem ser classificadas quanto ao seu nível de elaboração, assim como em aspectos ligados ao seu uso, como o formato de apresentação e de ações prévias. Os resultados nos ajudam a perceber situações de uso de analogias prioritariamente figurativas com alguns casos de analogias mecânicas. A ausência de uso de analogias matemáticas, assim como do formato de apresentação matemático-representativo, nos permite reafirmar o caráter crucial de se investigarem novos \"formatos\" de analogias que sejam adequados à comunicação e ao ensino de conceitos da física quântica.
This work deals with the use of analogies in teaching activities of sciences and, more specifically, with the use of analogies in high school quantum physics courses. Our starting point is the apparent contradiction of attempting to approach the concepts of quantum physics, abstract and deprived of any relation with the sensory reality, through a mechanism of representation based on the comparison of two fields knowledge: a first one which is known and familiar, and a second one which we wish to grasp. Our main contribution lies in our initial proposition to reflect on the concept of analogy from three different perspectives: first, there is the point of view of cognitive psychology, in which analogy appears as central element of thought and therefore of the formation of concepts during the whole history of the subject; second, there is the point of view of epistemology of science, and particularly that of Henri Poincaré who distinguished three basic categories for analogies (figurative, mechanical and mathematical); third, there is the point of view of educational activities, which considers analogies as a learning tool and attempts, through collective efforts manifested in the science education literature, to provide a systematic account of their use. Based on an ample reading along the three aforementioned dimensions, we then propose a synthesis enabling us to adopt a specific look at our object of research. In order to analyse the potential of this theoretical synthesis, we have conducted an empirical test constituted by episodes created within high school quantum physics lectures (atomic models, the photoelectric effect, the wave-particle duality, and the interpretations of Quantum Mechanics). In order to interpret these episodes, the methodology assumes that analogies in teaching activities can effectively be classified according to their level of development and to some aspects of their use, such as the presentation format and the previous actions. The results help us in perceiving how most of the analogies used are figurative, even though there are also some mechanical analogies. The lack of use of mathematical analogies, as well as the absence of the mathematical-representative presentation format, allows us to reaffirm the crucial need of investigating new \"formats\" of analogies, better suited for the communication and teaching of the concepts of quantum physics.

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Nobileau, Constance Médioni Michel. "Évaluation de la recherche des facteurs de risque cardiovasculaires en prévention primaire et en soins primaires à partir des résultats d'une évaluation des pratiques professionnelles (EPP) d'une association de formation médicale continue (FMC) de la ville de Créteil." Créteil : Université de Paris-Val-de-Marne, 2008. http://doxa.scd.univ-paris12.fr:80/theses/th0487208.pdf.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Vasseur, Julien. "Fiabilité prévisionnelle de la tenue en service de composants mécaniques en présence d’amas de porosités détectés et caractérisés par contrôle non destructif ultrasonore." Thesis, Ecole nationale supérieure Mines-Télécom Lille Douai, 2020. http://www.theses.fr/2020MTLD0002.

Повний текст джерела

Анотація:

Ces travaux de thèse ont pour objectif de définir une méthodologie permettant d’estimer de manière réaliste la durée de vie d’une structure en alliage d’aluminium (aluminium 7075-T6) contenant des amas porosités. Le choix technique retenu vise à détecter et caractériser ces porosités au moyen de contrôles non destructifs (CND) par ultrasons et de corréler les images ultrasonores obtenues à des mesures de fatigue. Dans le cadre de nos travaux, ce problème est ramené à une étude 2D où l’amas de porosités est représenté par un ensemble de trous-génératrices usinés, dont les caractéristiques géométriques sont totalement contrôlables, contrairement à celles de la porosité réelle. En partie ultrasonore, un effort particulier est apporté dans la prise en compte des effets de diffusion multiple existant entre les défauts lors du contrôle. Une étude comparative du modèle d’imagerie ainsi développé est menée en confrontant nos simulations à d’autres simulations d’imagerie simplifiées (effectuées sur le logiciel CIVA) mais également à des mesures expérimentales. En partie fatigue, une résolution d’un cas très complexe s’avère nécessaire, puisqu’il implique des possibilités de multi-amorçage et de couplage local de modes de sollicitation. Ce problème de la propagation de la fissure en fatigue est résolu par la méthode G-Ө. Nous montrons l’efficacité de la méthodologie employée en comparant les résultats de durée de vie qu’elle permet de prédire et ceux trouvés expérimentalement par essais de fatigue. Enfin, afin de faire le lien entre le domaine du CND et celui de la mécanique de la rupture, nous présentons une méthode permettant d’estimer la tenue en fatigue d’une éprouvette au vu de son imagerie obtenue suite à son contrôle ultrasonore. Cette démarche permet ainsi de proposer une méthodologie originale couplant le contrôle ultrasonore et la fatigue, en mettant en place un chaînage entre les deux domaines pour la fiabilisation de la prédiction de la durée de vie des structures en fatigue
This work aims at defining a methodology for the lifetime estimation of aluminum alloy structures (aluminum 7075-T6) according to the properties of porosity clusters contained in the sample. We chose to use advanced ultrasonic imaging techniques to detect and characterize these porosities and then to correlate the ultrasonic images obtained with fatigue measurements. In the framework of our study, the problem is simplified with a 2D study where the cluster of porosities is represented as a set of side-drilled holes (SDH) whose geometric characteristics are fully controllable, unlike those of real porosity. A special effort is made to estimate the effects of multiple scattering due to wave interactions between defects. A comparative study of the imaging model thus developed is carried out by comparing our simulations including multiple scattering with other simplified simulation results (carried out on CIVA software) but also with experimental measurements. Concerning fatigue study, a phenomenological approach is suggested to take into account multi-cracks initiation and local multi-mode behaviour generated by the cluster of defects. Therefore, crack propagation is solved by a G-Ө finite element method for prediction of the fatigue lifetime of different samples with different cases of cluster of defects. Numerical and experimental results were compared showing that the suggested methodology gives realistic lifetimes despite of complexity of the studied cases. Finally, the overall originality of this thesis consists in making a direct link between NDT results and fatigue calculation. Thus, a method for estimating the fatigue life of a specimen based on inversed ultrasonic images of defects is suggested and applied on some studied cases

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Pujol, Nadal Ramon. "Comportamiento Óptico y Térmico de un Concentrador Solar Lineal con reflector estacionario y Foco Móvil." Doctoral thesis, Universitat de les Illes Balears, 2012. http://hdl.handle.net/10803/84115.

Повний текст джерела

Анотація:

El concentrador solar Fixed Mirror Solar Concentrator (FMSC) apareció en los años 70 con la finalidad de reducir costes en la producción de energía termoeléctrica. Este diseño consiste en un concentrador de reflector estacionario y foco móvil, presenta buena integrabilidad en cubiertas, y es capaz de alcanzar temperaturas entre 100 y 200ºC manteniendo una eficiencia aceptable. En esta tesis se expone una metodología para determinar el comportamiento del FMSC. Se ha desarrollado una herramienta de cálculo basada en el método de ray-tracing, que simula el trazado de los rayos solares en el sistema óptico. Con esta herramienta se ha analizado el comportamiento óptico y térmico del FMSC, y de la versión con espejos curvos Curved Slats Fixed Mirror Solar Concentrator (CSFMSC). Se ha realizado un análisis paramétrico para conocer la influencia de los distintos parámetros en el modificador de ángulo (IAM), y para obtener los diseños óptimos a una temperatura de 200ºC para tres climas en diferentes latitudes. Se han comparado los valores teóricos obtenidos mediante ray-tracing con dos prototipos ensayados, obteniendo un buen ajuste en ambos casos. Los ensayos han sido utilizados para determinar la curva de rendimiento de uno de los prototipos. Se ha hecho uso del método propuesto en la norma EN-12975-2:2006, combinado con valores de IAM obtenidos mediante ray-tracing. Se prueba que esta combinación puede ser útil para obtener la curva de rendimiento de colectores complejos con un modelo biaxial para el IAM.
The Fixed Mirror Solar Concentrator (FMSC) appeared during the 70s with the aim of reducing costs in the production of electricity in solar thermal power plants. This design consists of a concentrator with fixed reflector and moving receiver, has a very good integrability into building roofs and can reach temperatures between 100 and 200ºC with an acceptable efficiency. In this Thesis a methodology is presented for the determination of the behaviour of the FMSC. A simulation tool based on the forward ray-tracing method has been developed. The optical and thermal behaviour of the FMSC and its curved mirror variation called the Curved Slats Fixed Mirror Solar Concentrator (CSFMSC), have been analyzed with this tool. A parametric analysis has been carried out in order to determine the influence of the different parameters on the Incidence Angle Modifier (IAM) and to determine the optimal designs at a temperature of 200ºC for three different climates at different latitudes. The theoretical values obtained from the ray-tracing code have been compared with two experimental prototypes. The experimental and numerical results obtained show a good fit. The efficiency curve of one of the prototypes has been determined from the experimental tests. The methodology proposed in the norm EN-12975-2:2006 has been used in combination with IAM values obtained by ray-tracing. It has been shown that this combination can be effectively used to obtain the efficiency curve of complex collectors with a bi-axial IAM model.

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Ghimire, Harishchandra. "Structure, Dynamics, and Distance Measurements in Membrane Proteins and Peptides using EPR Spectroscopic Techniques." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1291739688.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

49

"Conceptual architecture patterns : FMC–based representations." Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2009/3317/.

Повний текст джерела

Анотація:

This document presents the results of the seminar "Coneptual Arachitecture Patterns" of the winter term 2002 in the Hasso-Plattner-Institute. It is a compilation of the student's elaborations dealing with some conceptual architecture patterns which can be found in literature. One important focus laid on the runtime structures and the presentation of the patterns. 1. Introduction 1.1. The Seminar 1.2. Literature 2 Pipes and Filters (André Langhorst and Martin Steinle) 3 Broker (Konrad Hübner and Einar Lück) 4 Microkernel (Eiko Büttner and Stefan Richter) 5 Component Configurator (Stefan Röck and Alexander Gierak) 6 Interceptor (Marc Förster and Peter Aschenbrenner) 7 Reactor (Nikolai Cieslak and Dennis Eder) 8 Half–Sync/Half–Async (Robert Mitschke and Harald Schubert) 9 Leader/Followers (Dennis Klemann and Steffen Schmidt)

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Zizzo, Carmela. "Familial Mediterranean Fever (FMF) or Fabry disease? Genetic analysis and study of GLA as “modifier gene” of FMF." Doctoral thesis, 2012. http://hdl.handle.net/10447/94892.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Дисертації з теми "FMFC"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями