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Статті в журналах з теми "Fluorescenza della Chl a"
MORO, A., C. DE WAURE, F. DI NARDO, F. SPADARI, M. D. MIGNOGNA, M. GIULIANI, L. CALIFANO, et al. "Il dispositivo medico GOCCLES® è in grado di individuare displasie e cancro orale se impiegato nel setting odontoiatrico. Risultati da uno studio multicentrico." Acta Otorhinolaryngologica Italica 35, no. 6 (December 2015): 449–54. http://dx.doi.org/10.14639/0392-100x-922.
Повний текст джерелаDi Lullo, A. M., M. Scorza, F. Amato, M. Comegna, V. Raia, L. Maiuri, G. Ilardi, E. Cantone, G. Castaldo, and M. Iengo. "An ex vivo model contributing to the diagnosis and evaluation of new drugs in cystic fibrosis." Acta Otorhinolaryngologica Italica 37, no. 3 (June 2017): 207–13. http://dx.doi.org/10.14639/0392-100x-1328.
Повний текст джерелаQuaranta, Luca, Piera Di Marzio, Romeo Di Pietro, Fabrizio Ferretti, Umberto Di Salvatore, and Paola Fortini. "Analysis of the functional traits of Quercus cerris L. seedlings in the Molise region (southern Italy)." Plant Sociology 59, no. 1 (May 16, 2022): 11–24. http://dx.doi.org/10.3897/pls2022591/02.
Повний текст джерелаNiroumand-Jadidi, Milad, Francesca Bovolo, and Lorenzo Bruzzone. "Water Quality Retrieval from PRISMA Hyperspectral Images: First Experience in a Turbid Lake and Comparison with Sentinel-2." Remote Sensing 12, no. 23 (December 6, 2020): 3984. http://dx.doi.org/10.3390/rs12233984.
Повний текст джерелаДисертації з теми "Fluorescenza della Chl a"
Gottardini, Elena. "Risposte morfologiche, fisiologiche e geniche all’ozono della specie arbustiva Viburnum lantana L." Doctoral thesis, country:IT, 2012. http://hdl.handle.net/10449/22868.
Повний текст джерелаRubino, Vincenzo. "Studio della dinamica di perfusione per la valutazione della endometriosi ureterale." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2021. http://amslaurea.unibo.it/23018/.
Повний текст джерелаPalladino, Giorgia. "Valutazione della localizzazione e della tossicità di nanoparticelle di silice fluorescenti su linee cellulari tumorali umane." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/11152/.
Повний текст джерелаPiccotto, Massimo. "Effetti degli NOx sulla fisiologia dei licheni foliosi epifiti." Doctoral thesis, Università degli studi di Trieste, 2009. http://hdl.handle.net/10077/3170.
Повний текст джерелаL’obiettivo della ricerca è stato individuare i possibili effetti degli NOx, inquinanti aerodiffusi foto-ossidanti, sui licheni foliosi epifiti. Il lavoro è stato svolto con un particolare riguardo nell’individuare le modificazioni indotte a carico del processo fotosintetico del fotobionte lichenico attraverso metodi fluorimetrici. Le ricerche condotte hanno approfondito inizialmente alcuni aspetti metodologici, individuando, ad esempio, le variabili ambientali che influenzano maggiormente la capacità e l’efficienza fotosintetica di questi organismi. Successivamente, è stata disegnata una articolata sperimentazione, condotta mediante trapianti in siti urbani inquinati e non, che ha permesso di verificare gli effetti degli inquinanti aerodiffusi a concentrazioni ambientali in presenza di altri, naturali, fonti di disturbo. Questo lavoro dimostra, attraverso misure fisiologiche, che l’arido microclima urbano può essere un fattore limitante la fisiologia dei licheni e che la loro tolleranza agli NOx dipende strettamente dalla loro ecologia.
XXI Ciclo
1980
CICCONE, SARAH. "Studio dei polimorfismi delle glutatione trasferasi nell'aumentata suscettibilità ai processi tumorali: caratterizzazione strutturale e funzionale della glutatione trasferasi di cianobatterio." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2010. http://hdl.handle.net/2108/1219.
Повний текст джерелаGlutathione S-transferases (GSTS) are considered part of a coordinated defence strategy, together with other GSH-dependent enzymes, the cytochrome P450s (Phase I enzymes) and some membrane transporters (Phase III) such as MRP1 and MRP2, to remove from the cell the products of oxidative stress generated after interaction of reactive oxygen species, that escape the first line of defense, with cellular macromolecules such as DNA, lipids and proteins. Glutathione S-transferases (EC 2.5.1.18) catalyze the conjugation of glutathione (GSH) with a variety of toxic compounds (carcinogens, anticancer drugs, reactive oxygen species and products of cellular metabolism) that contain an electrophilic atom, i.e. carbon, nitrogen or sulphur. In mammals, there are three major families of proteins that exhibit glutathione transferase activity: two of these, the cytosolic and mitochondrial GSTs, comprise soluble enzymes, while the third family are microsomal (MAPEG) and are referred to as membrane-associated proteins in eicosanoid and glutathione metabolism (Hayes et al., 2005). The human cytosolic GSTs are dimeric proteins; each subunit contains a very similar binding site for GSH (G-site) and a second one for the hydrophobic co-substrate (H-site). Structural differences at the H-site confer a certain degree of substrate selectivity. The human cytosolic GSTs can be grouped into at least seven gene-independent classes on the basis of their amino acid sequence and immunological properties: Alpha, Mu, Pi, Sigma, Theta, Omega, and Zeta Cytosolic GSTs display polymorphisms in humans, and this is likely to contribute to interindividual differences in responses to xenobiotics . A lot of studies suggest that that combinations of polymorphisms in Mu, Pi, and Theta class GST contribute to diseases development. In the first part of this work we analyzed three common polymorphisms in the GSTP1, GSTT1, and GSTM1 genes either decrease or abolish GST enzyme activity: the GSTP1 allelic variants, that differ at either a single codon position (Ile104 (HGSTP1*A), Val104 (HGSTP1*B), Val113 HGSTP1*D) or at two different positions (Val104/Val113 (HGSTP1*C)), and the homozygous deletions of the GSTT1 or GSTM1 gene that lead to an absence of enzymatic activity. The commonly used anti-cancer drug chlorambucil is the primary treatment for patients with chronic lymphocytic leukaemia. Chlorambucil has been shown to be detoxified by human glutathione transferase Pi (GST P1-1), an enzyme that is often found over-expressed in cancer tissues. The allelic variants of GST P1-1 are associated with differing susceptibilities to leukaemia and differ markedly in their efficiency in catalysing glutathione (GSH) conjugation reactions. Here, we perform detailed kinetic studies of the allelic variants with the aid of three representative co-substrates. We show that the differing catalytic properties of the variants are highly substrate-dependent. We show also that all variants exhibit the same temperature stability in the range 10 °C to 55 °C. We have determined the crystal structures of GST P1-1 in complex with chlorambucil and its GSH conjugate for two of these allelic variants that have different residues at positions 104 and 113. Chlorambucil is found to bind in a non-productive mode to the substrate-binding site (H-site) in the absence of GSH. This result suggests that under certain stress conditions where GSH levels are low, GST P1-1 can inactivate the drug by sequestering it from the surrounding medium. However, in the presence of GSH, chlorambucil binds in the H-site in a productive mode and undergoes a conjugation reaction with GSH present in the crystal. The crystal structure of the GSH–chlorambucil complex bound to the *C variant is identical with the *A variant ruling out the hypothesis that primary structure differences between the variants cause structural changes at the active site. Finally, we show that chlorambucil is a very poor inhibitor of the enzyme in contrast to ethacrynic acid, which binds to the enzyme in a similar fashion but can act as both substrate and inhibitor. In another part of this work we determined in the peripheral blood lymphocytes of 157 workers exposed to benzene, using 25 individuals not exposed as external controls, the presence of polymorphic genes GSTT1 and GSTM1 and the distribution of GSTP1 allelic variants. We have also evaluated the glutathione transferases (GST) activities and the levels of glutathionylated hemoglobin in the RBC of the same samples. Because this study is in progress again, we can’t estabilish the finals conclusions. During my Phd project I have also analyzed the presence of GSTP1-1 enzyme in prostatic cancer cells lines. In contrast to frequent overexpression of GST-pi observed in many types of cancer, the vast majority of primary human prostate tumors contain no detectable GST pi. So, this enzyme is abundant in normal prostate basal epithelial cells, but basal cells are lost during development of invasive cancer. Absence of GST pi expression in human prostate cancer is accompanied by hypermethylation of regulatory sequences within the GST pi gene, whereas no such hypermethylation is present in normal tissues or benign prostatic hyperplasia (HPB). So we employed Western blot analysis and specific activity assays to measure GST pi expression in diferrents prostatic cancer cells lines selected on the basis of their tumor staging. We have analyzed HBP lines (positive controls), G1 cells lines (associated with a negative prognosis), G2 cells lines (associated with good prognosis) and LNCaP (negative controls). At the and of this study we observed that the most relevant expression of GST pi was detectable in HPB cells, but also in G2 and G1 cells is possible to note a very low presence of this enzyme. The last part of my project comprehend the purification and the enzymatic caratherization of a new GST, called GST short, sequenzed by the cyanobacteria Synechocystis sp PCC 6803 genome. Cyanobacteria represent a group of widely distributed prokaryotes performing oxygenic photosynthesis similar to plants. This, together with their generally accepted role as progenitors of plant plastids, and their ease of genetic manipulation, has made them extremely useful in studies of environmental gene regulations and the mechanism of oxygenic photosynthesis. In according to its phylogenetic origin, GST short presents a very similar G-site sequence with the sequence previously described for GSTI and GSTIII of Zea mays. It is also active towards several classical substrates, but at the same moderate rates that have been observed for other glutathione transferases derived from prokaryotes. Particulary, it was possible observe a strong perossidase activity. We have also analyzed the GST short thermal stability respect to hGSTP1-1 (10°-55°C) and the results indicate that the cyanobacterial enzyme is less resistant at this temperatures than human enzyme probably because its different structure. The cloning, expression and characterization of this cyanobacterial glutathione transferase is also described . The possible significance of the observed catalytic properties is discussed in the context of structural organization and glutathione transferase evolution.
TAGLIABUE, GIULIA. "Linking vegetation optical properties from multi-source remote sensing to plant traits and ecosystem functional properties." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2019. http://hdl.handle.net/10281/241317.
Повний текст джерелаRemote Sensing (RS) data have been successfully exploited in the last decades to monitor vegetation due to their inherent capacity of providing repeated and spatially-distributed quantitative information about vegetation properties. However, most research focused on the description of the structural and biochemical properties of vegetation rather than on the understanding of its functioning. In the last decade, RS of sun-induced chlorophyll fluorescence (F) emerged as a novel and promising tool for assessing plant functional status. F is a weak signal emitted by the core of the photosynthetic machinery in the red and far-red spectral regions (~650-800 nm) as a side product of light absorption. The potential of F relies on the relationship existing between photochemistry and the energy dissipation pathways: since photochemistry competes with F emission and heat dissipation for the absorbed energy, F can be a direct indicator of plant actual functional state. The main aim of this Ph.D. research was to exploit optical data (i.e., reflectance and fluorescence) to advance the understanding of vegetation functioning and of its variability across space. In particular, the work aimed at better understanding the link between vegetation optical properties, plant traits (PTs) and ecosystem functional properties (EFPs) in a case study represented by a mid-latitude forest ecosystem. At this purpose, innovative RS techniques were exploited to infer information about the vegetation functioning from fine and ultra-fine spectral resolution optical measurements acquired with the HyPlant airborne imaging spectrometer. The analyses were focused on two main work streams: i) the investigation of the spatial relationship between F and EFPs to better understand the variability of the ecosystem functioning at regional scale; ii) the analysis of the potential of F as a synthetic descriptor of the ecosystem functional diversity. Results provided evidence of the effectiveness of F as a tool for assessing vegetation functioning, but also pointed out the complexity of the link existing between F, PTs and EFPs and the need to integrate different RS derived products to obtain an unambiguous interpretation of the F signal. In particular, results showed that: i) F can be related to the spatial variability of the EFPs, thus demonstrating that this link usually observed in the temporal domain holds in the spatial domain; ii) F is a more powerful tool compared to traditional reflectance-based indices for explaining the functional diversity. Overall, these results improved the understanding of the complex relationship between F and vegetation functioning by adding new insights into the critical role of the spatial heterogeneity in controlling the carbon uptake. Further research in this direction constitutes a high priority for advancing the understanding of the imprint of plants on the global carbon balance.
Florio, Francesco Paolo. "Studio delle modificazioni strutturali della matrice vegetale mela a seguito di differenti intensità di campi elettrici pulsati (PEF)." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2019.
Знайти повний текст джерелаPASQUA, IRENE. "Significato clinico dell'espressione della proteina ZAP-70 nelle leucemie linfatiche croniche." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2009. http://hdl.handle.net/2108/1024.
Повний текст джерелаDysfunctional apoptosis and cell cycle are the main reasons for the clinical enigma, that CLL can not yet be cured with conventional chemotherapy. In B-CLL, malignant cells seem to be arrested in the G0/early G1 phase of the cell cycle, and inhibition of spontaneous apoptosis and upregulation of the anti-apoptotic protein bcl-2 define clinical prognosis. However, increasing evidence exists that disease progression relies upon cycling B-CLL cells: a proliferating pool of cells has been described in lymph nodes and bone marrow and might feed the accumulating pool in the blood. Moreover, the lack of immunoglobulin (Ig) VH gene mutation also has been shown to predict a rapid disease progression (DP) and an inferior overall survival (OS) (Damle, Hamblin, 1999). B-CLL cells that use non-mutated IgVH genes express ZAP-70 RNA, which encodes ZAP-70, a 70-kDa protein tyrosine kinase, associated both with an enhanced B cell receptor signaling and with an early DP risk in B-CLL (Del Principe, 2006). Moreover, the today availability of rapamycin or proteasome inhibitors effective against proliferating B-CLL cells and bcl-2 antisense oligonucleotides prompted us to evaluate the real impact of proliferation and apoptosis pathways on B-CLL prognosis. The primary aims of our study were: 1) to determine progression-free survival (PFS) upon apoptosis/proliferation subgroups and ZAP-70 expression; 2) whether apoptosis/proliferation could predict varied outcome within ZAP-70 subgroups; and finally 3) whether ZAP-70 and apoptosis/proliferation groups were independent prognostic factors. Therefore we investigated 265 pts, median age 64 years (range 37-84), 136 males and 129 females. With regard to modified Rai stages, 87 patients had a low stage, 170 an intermediate stage and 8 a high stage. ZAP-70 was quantified by a multicolor flow cytometric method fixing a cut-off value of 20%. Bcl-2 was determined by flow cytometry, dividing mean fluorescence intensity (MFI) of CD19+B-CLL cells / MFI of T-cells (Bcl-2B/T). The threshold was set at the median value >1.6. Transferrin receptor (CD71) was used as a measure of the proliferation and the threshold was set at the median value >8%. Combining Bcl-2B/T with CD71 (Bcl2CD71) we enucleated three subgroups: 1) Bcl2CD71- [106 pts] with low proliferation (CD71 <8%) and high apoptosis (Bcl-2B/T <1.6); 2) Bcl2CD71+ [49 pts] with high proliferation (CD71>8%) and low apoptosis (Bcl-2B/T >1.6); and 3) Bcl2CD71+/- [110 pts] with low proliferation and low apoptosis or with high proliferation and high apoptosis. ZAP-70+ B-CLL patients were 95/265 (36%). In 111 studied pts ZAP-70 expression and Ig V gene mutational status were significantly correlated (p<0.00001). Furthermore, we found significant associations either between lower ZAP-70 and lower Bcl-2B/T index (p=0.001) or lower ZAP-70 and Bcl2CD71- (p=0.002), confirming that low levels of ZAP-70 were characterized by high apoptosis and low proliferation. With regard to clinical outcome, a significant shorter progression-free survival (PFS) was observed in ZAP-70+ pts vs ZAP-70 negative pts (0% vs 58% at 13 years; p<0.00001) and in Bcl2CD71+ pts vs Bcl2CD71- pts (10% vs 56% at 12 years; p<0.00001). The Bcl2CD71+/- subgroup showed an intermediate outcome (30% at 12 years). To further explore the prognostic impact of Bcl2CD71 index, we investigated its expression within ZAP70+ (95 pts) and ZAP70- (170 pts) subsets. As a matter of fact, Bcl2CD71 was not able to identify prognostic subsets within ZAP-70+ pts, because all these cases presented a shorter PFS without significant differences. On the other hand, this index identified subsets at different PFS within the ZAP-70 negative subgroup (73% for Bcl2CD71- pts vs 29% for Bcl2CD71+ at 12 years, p=0.00009). In multivariate analysis of PFS, in which age, Rai modified stages, CD38, soluble CD23 (sCD23), lymphocyte doubling time (LDT), Bcl-2CD71 and ZAP-70 entered, ZAP-70 (p=0.00005), LDT (p=0.006), Rai modified stages (p=0.03) and sCD23 (p=0.01) resulted to be independent prognostic factors. Therefore, ZAP-70 was confirmed as the most important indipendent prognostic factor with regard to PFS. However, our apoptotic/proliferative index (Bcl2CD71), performed by flow cytometry, was very useful to identify pts at different progression rate within the ZAP-70 negative subgroup. Since the ZAP-70 negative subset represents a large and heterogeneous B-CLL population with a variable progression, other biological factors, such as the amount of apoptosis and the proliferative rate, have to be added in order both to identify early progressive pts and to take timely accurate therapeutic decisions.
FRACASSO, ALESSANDRA. "Caratterizzazione di genotipi di sorgo in funzione di tratti legati alla tolleranza alla siccità." Doctoral thesis, Università Cattolica del Sacro Cuore, 2015. http://hdl.handle.net/10280/6073.
Повний текст джерелаDue to the increasing human population and the consequent surging energy and water demand, it is necessary to implement energy and fuel production from low input renewable sources. Sweet sorghum (Sorghum bicolor Moench) is a low input multipurpose crop that provides food, feed and bioethanol from conversion of sugars accumulated in the stalk and biogas from anaerobic digestion of whole aboveground dry biomass. This multipurpose crop was studied in response to water deficit. In particular, the biomass production and its composition were evaluated in response to drought for new developed and commercial genotypes for biogas and bioethanol production. The physiologic and molecular approaches were combined in order to provide an integrated view on drought tolerance in sorghum enabling to know which are the mechanisms and with which extent they were affected by drought in this bioenergy crop. The transcriptomic analysis was performed on two sorghum genotypes (one sensitive and the other one tolerant to drought) with RNA-Seq technology in order to evaluate the diversity existing in the sorghum transcriptome that could be related to drought tolerance and to identify candidate genes that could be used as potentially marker for the marker assisted selection.
FRACASSO, ALESSANDRA. "Caratterizzazione di genotipi di sorgo in funzione di tratti legati alla tolleranza alla siccità." Doctoral thesis, Università Cattolica del Sacro Cuore, 2015. http://hdl.handle.net/10280/6073.
Повний текст джерелаDue to the increasing human population and the consequent surging energy and water demand, it is necessary to implement energy and fuel production from low input renewable sources. Sweet sorghum (Sorghum bicolor Moench) is a low input multipurpose crop that provides food, feed and bioethanol from conversion of sugars accumulated in the stalk and biogas from anaerobic digestion of whole aboveground dry biomass. This multipurpose crop was studied in response to water deficit. In particular, the biomass production and its composition were evaluated in response to drought for new developed and commercial genotypes for biogas and bioethanol production. The physiologic and molecular approaches were combined in order to provide an integrated view on drought tolerance in sorghum enabling to know which are the mechanisms and with which extent they were affected by drought in this bioenergy crop. The transcriptomic analysis was performed on two sorghum genotypes (one sensitive and the other one tolerant to drought) with RNA-Seq technology in order to evaluate the diversity existing in the sorghum transcriptome that could be related to drought tolerance and to identify candidate genes that could be used as potentially marker for the marker assisted selection.