Дисертації з теми "Flow cytometers"
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Bausch, Cornelius Sebastian [Verfasser]. "Tubular coplanar waveguides for the use in radio-frequency microfluidic flow cytometers / Cornelius Sebastian Bausch." München : Verlag Dr. Hut, 2016. http://d-nb.info/1103872044/34.
Повний текст джерелаLe, Lann Lucas. "Elaboration d'une procédure standardisée d'harmonisation des données de cytométrie en flux dans le cadre d'études multicentriques Multi-center harmonization of flow cytometers in the context of the European “PRECISESADS” project, in Autoimmunity Reviews 15(11), November 2016." Thesis, Brest, 2019. http://www.theses.fr/2019BRES0048.
Повний текст джерелаThe aim of this thesis is to ensure the comparability of flow cytometry data within the context of multi-center studies. This thesis work is part of the PRECISESADS project. This European project seeks to reclassify the systemic autoimmune diseases using "omic" data to find useful biological signatures. This encompasses tools like genomic, proteomic ... and flow cytometry. The inclusion of numerous individuals in the project make the use of informatics tools a must for the analysis automation of the thousands flow cytometry files obtained during the 5-years period of the project.Cell populations frequencies extracted from the files are comparable between centers but that is not the case for the median of fluorescence intensities (MFI) of the studied molecules, despite a normalization step. The origin of this incomparability is due to a combination of a batch effect and a center effect. Those two effects can be corrected with specific coefficients. The normalization and correction of both batch and center effect thanks to the elaboration of new R script and python script allow the production of comparable MFI between centers. Overall, this thesis work established a new standardized procedure, efficient for any multi-center projects of flow cytometry data analysis
Venkatesh, Mukung C. (Mukund Chakravarthy). "Optimization of the mini-flo flow cytometer dc by Mukund C. Venkatesh." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/40009.
Повний текст джерелаDavey, Hazel Marie. "Flow cytometry of microorganisms." Thesis, Aberystwyth University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309050.
Повний текст джерелаNovak, John P. (John Peter) 1957. "Development of the in vivo flow cytometer." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30331.
Повний текст джерелаIncludes bibliographical references.
An in vivo flow cytometer has been developed that allows the real-time detection and quantification of circulating cells containing fluorescent proteins or labeled with fluorochrome molecules in live animals, without the need to extract blood samples. A stationary laser beam is focused by a cylindrical lens to a slit of light that is then demagnified and focused across a blood vessel by an achromat and microscope objective. Fluorescent cells are excited one by one as they flow through the excitation laser light slit, creating a burst of fluorescence whose width is inversely proportional to their velocity. The fluorescence signal is detected through a confocal slit aperture using a photomultiplier tube. The analog signal from the photomultiplier tube is then digitized, filtered, and recorded as a function of time onto a computer. Computer programs post- process the data for the presence of cell signal, as well as various aspects of the cell signal such as height, width, and temporal location of the signal peak. Two in vivo flow cytometers have been built: a single-slit, single-color system and a two- slit, two-color system. The single-slit, single-color system provides excitation at 632 nm, and the two-slit, two-color system provides excitation at 632 nm and 473 nm. The two- slit, two-color system can operate in several different modes: single-slit at 632 nm or 473 nm, double-slit at 632 nm or 473 nm, and double-slit with one excitation slit at 632 nm and the other at 473 nm.
(cont.) Thus far, the single-slit, single-color system has been used to study the circulation kinetics of different prostate cancer and leukemia cell lines with different metastatic potential, as well as the effect of different host environments (i.e., mouse versus rat). In addition, the device has been used to develop a new in vivo labeling method of white blood cells that does not result in significant depletion of the labeled cells, allowing for the possibility of autoimmune and transplant rejection studies. The two-slit, two-color system is being used to track two different cell populations, or one cell population labeled with two different markers, one of which can be the green fluorescent protein.
by John P. Novak.
Ph.D.
Wållberg, Fredrik. "Flow cytometry for bioprocess control." Licentiate thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1736.
Повний текст джерелаDuring bio-technical processing it is important to monitorbiological parameters such as cell growth, viability andproduct formation. Many of the analyses traditionally used areslow to perform and provide only average data for thepopulation. Flow cytometry is a laser-based technique, whichmeasures physical properties of a cell in a flowing stream, ata rate of several thousand cells per second. It offers theprospect of an at-line, multi-parameter analysis of individualmicroorganisms in a population.
In this project several methods for at-line measurements ofbioprocesses were developed such as protocol's for measuringcell concentration, viability and product formation. Theprimary focus was on prokaryotic organisms (E. coli) but eukaryotic organisms (P. pastoris) were included.
The possibility to use volumetric cell counting to measurecell concentration (cell number) was evaluated. It was shownthat the method was applicable for high cell density processesof bothE. coliandP. pastoris.
The combination of Bis- (1,3-dibutylbarbituric acid)trimethine oxonol (depolarised membranes) and propidium iodide(loss of membrane integrity) as fluorescent markers was usefulto measure viability at-line of cells in high cell densityprocesses. The protocol was shown to be reproducible forE. coliandP. pastoris.
The viability staining was used to study the kinetics ofweak organic acids (food preservatives). The protocol provideddata about cell functions such as membrane depolarisation andloss of membrane integrity caused by introducing weak organicacids to shake flask cultures ofE. coli.
Labeling inclusion bodies with fluorescent antibodiesprovided a method, which could specifically monitor theincreased accumulation of recombinant promegapoetin proteinwith process time. This technique was further developed forintracellular staining by application of a permeabilising stepbefore labeling with antibodies. Staining of inclusion bodiesdirectly inside permeabilised cells gave information about thedistribution of protein expression in the cell population.
In conclusion, flow cytometry provides an at-line, singlecell technique for measurement of several biological parametersin bioprocesses.
Key words: flow cytometry, Partec PAS, propidium iodide(PI), bis- (1,3-dibutylbarbituric acid) trimethine oxonol(BOX), Alexa fluor 488, bioprocess,E. coli,P. pastoris, inclusion body, food preservatives,viability, membrane potential
Helou, Michael [Verfasser]. "Magnetic Flow Cytometry / Michael Helou." Kiel : Universitätsbibliothek Kiel, 2018. http://d-nb.info/1169132596/34.
Повний текст джерелаStewart, Justin William. "Photonic Crystal-Based Flow Cytometry." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5396.
Повний текст джерелаSobek, Daniel. "Microfabricated fused silica flow chambers for flow cytometry." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/10262.
Повний текст джерелаIncludes bibliographical references (leaves 107-116).
by Daniel Sobek.
Ph.D.
Collins, Gary Stephen. "Multivariate analysis of flow cytometry data." Thesis, University of Exeter, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324749.
Повний текст джерелаShooshtari, Parisa. "Computational techniques for flow cytometry : the application for automated analysis of innate immune response flow cytometry data." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/42179.
Повний текст джерелаCondrau, Marc Anton. "Time-resolved fluorescence measurement in flow cytometry /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10267.
Повний текст джерелаPorter, Jonathan David. "Applications of flow cytometry to bacterial ecology." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385323.
Повний текст джерелаHeiden, Thomas. "Clinical fluorescence cytometry : improvements to preparation methods and instrumentation /." Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3648-X/.
Повний текст джерелаHerrala, S. (Sauli). "Level set clustering in a flow cytometry dataset." Master's thesis, University of Oulu, 2016. http://urn.fi/URN:NBN:fi:oulu-201604191509.
Повний текст джерелаHelstad, Amanda. "Application of Flow Cytometry for Slow Sand Filters." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-157760.
Повний текст джерелаFördröjning av publikation fram till 31 december 2020.
Buldini, Barbara. "Flow cytometry application in hematological malignancies of childhood." Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425984.
Повний текст джерелаHuang, Ming-Chieh. "Silicon microfabricated device for non-sheath-flow cytometer-based chemical analysis and microchannel flow sensing /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/5870.
Повний текст джерелаWu, Jianglai. "A light sheet based fluorescence imaging flow cytometer for phytoplankton analysis." HKBU Institutional Repository, 2014. https://repository.hkbu.edu.hk/etd_oa/36.
Повний текст джерелаMeyer, Michael [Verfasser]. "Applications of aptamers in flow cytometry assays / Michael Meyer." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2014. http://d-nb.info/1059512718/34.
Повний текст джерелаAlexander, R. G. "Flow cytometry and cell sorting in plant genetic manipulations." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356016.
Повний текст джерелаSpurgeon, Benjamin E. J. "Phosphoproteomic analysis of platelet signalling cascades by flow cytometry." Thesis, University of Hull, 2014. http://hydra.hull.ac.uk/resources/hull:10543.
Повний текст джерелаAutret, Arnaud. "Modular neural networks for analysis of flow cytometry data." Thesis, University of South Wales, 2003. https://pure.southwales.ac.uk/en/studentthesis/modular-neural-networks-for-analysis-of-flow-cytometry-data(49f3349b-e86a-4bfb-a689-c853323b6f2d).html.
Повний текст джерелаBRANCHI, FEDERICA. "THE ROLE OF FLOW CYTOMETRY IN COMPLICATED CELIAC DISEASE." Doctoral thesis, Università degli Studi di Milano, 2018. http://hdl.handle.net/2434/553600.
Повний текст джерелаFarahat, Nahla Mohamed Gamal. "Minimal residual disease in acute leukaemia by quantitative flow cytometry." Thesis, Institute of Cancer Research (University Of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244275.
Повний текст джерелаJepras, Robert Ian. "Applications of photon correlation spectroscopy and flow cytometry to microbiology." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290872.
Повний текст джерелаWant, Andrew James. "Physiological studies on bacterial fermentations using multi-parameter flow cytometry." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/1048/.
Повний текст джерелаVeale, Margaret Fiona. "Activated immune cells : 1H-NMR spectroscopy and flow cytometry studies." Thesis, The University of Sydney, 1996. https://hdl.handle.net/2123/27547.
Повний текст джерелаHedblom, Sofia. "Detection and quantification of fetal hemoglobin in blood using flow cytometry." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-176636.
Повний текст джерелаHall, Jerome Lynn. "Optical Properties of Marine Phytoplankton: A Study in Multiparameter Flow Cytometry." NSUWorks, 1989. http://nsuworks.nova.edu/occ_stuetd/364.
Повний текст джерелаLam, Yung-chun Nelson, and 林勇進. "Annual distribution of phytoplankton in Tolo Harbour: a flow cytometry approach." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B3124192X.
Повний текст джерелаDeans, Gordon Taylor. "The role of flow cytometry and image analysis in colorectal carcinoma." Thesis, Queen's University Belfast, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334468.
Повний текст джерелаAnvarian, Amir Hossein Pour-Taghi. "Physiology of Escherichia coli in orange juice : applications of flow cytometry." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/5653/.
Повний текст джерелаBliss, Jonathan G. "Rapid detemination of antimicrobial susceptibility using gel microdroplets and flow cytometry." Thesis, Massachusetts Institute of Technology, 1990. http://hdl.handle.net/1721.1/12797.
Повний текст джерелаIncludes bibliographical references (leaves 226-233).
by Jonathan G. Bliss.
Ph.D.
Shimada, Toshihide. "Detection of IgA binding site by flow cytometry with fluorescent microspheres." Kyoto University, 1992. http://hdl.handle.net/2433/168720.
Повний текст джерелаKyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第5141号
医博第1403号
新制||医||540(附属図書館)
UT51-92-K331
京都大学大学院医学研究科病理系専攻
(主査)教授 鈴木 康弘, 教授 内田 温士, 教授 杉山 武敏
学位規則第4条第1項該当
Lam, Yung-chun Nelson. "Annual distribution of phytoplankton in Tolo Harbour a flow cytometry approach /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22718874.
Повний текст джерелаSimone, Lucchesi. "Computational flow cytometry for characterizing the immune response in vaccine studies." Doctoral thesis, Università di Siena, 2020. http://hdl.handle.net/11365/1095903.
Повний текст джерелаMartini, V. "CLINICAL PATHOLOGICAL FEATURES OF CANINE HEMATOPOIETIC NEOPLASMS ASSESSED VIA FLOW CYTOMETRY." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/232394.
Повний текст джерелаPitzler, Christian Verfasser], Ulrich [Akademischer Betreuer] [Schwaneberg, and Andrij Z. [Akademischer Betreuer] Pich. "Novel flow cytometer-based platforms for directed evolution / Christian Pitzler ; Ulrich Schwaneberg, Andrij Pich." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1130590496/34.
Повний текст джерелаWelsh, Joshua. "Flow cytometer optimisation and standardisation for the study of extracellular vesicles as translational biomarkers." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/410614/.
Повний текст джерелаPitzler, Christian [Verfasser], Ulrich [Akademischer Betreuer] Schwaneberg, and Andrij Z. [Akademischer Betreuer] Pich. "Novel flow cytometer-based platforms for directed evolution / Christian Pitzler ; Ulrich Schwaneberg, Andrij Pich." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1130590496/34.
Повний текст джерелаOwen, Henry R. "Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement." Diss., This resource online, 1987. http://scholar.lib.vt.edu/theses/available/etd-07282008-135528/.
Повний текст джерелаMulhearn, Ben. "Identifying immune biomarkers to predict treatment response to biologic drugs in rheumatoid arthritis." Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/identifying-immune-biomarkers-to-predict-treatment-response-to-biologic-drugs-in-rheumatoid-arthritis(c311fc8c-4239-444a-9912-ddd4fde5f7fa).html.
Повний текст джерелаCullen, Matthew John. "The use of flow cytometry in the diagnosis of the Myelodysplastic Syndromes." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/13713/.
Повний текст джерелаBjörklund, Elisabet. "Multiparameter flow cytometry and minimal residual disease in patients with acute leukemia /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-624-3.
Повний текст джерелаLevering, Wilfridus Henricus Bernardus Maria. "External quality assessment in flow cytometry educational aspects and trends toward improvement /." [S.l.] : Rotterdam : [The Author] ; Erasmus University [Host], 2007. http://hdl.handle.net/1765/10602.
Повний текст джерелаZare, Habil. "Automatic analysis of flow cytometry data and its application to lymphoma diagnosis." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/39660.
Повний текст джерелаTu, Ran [Verfasser]. "Flow cytometry based screening systems for directed evolution of proteases / Ran Tu." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2010. http://d-nb.info/1035032872/34.
Повний текст джерелаKarlsson, Mathilda. "Can the proliferative ability of chicken cardiomyocytes be assessed using flow cytometry?" Thesis, Linköpings universitet, Biologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-128890.
Повний текст джерелаCOZZI, MARZIA. "FLOW CYTOMETRY FOR THE DIAGNOSIS AND THE CHARACTERIZATION OF CANINE LYMPHOPROLIPHERATIVE TUMORS." Doctoral thesis, Università degli Studi di Milano, 2018. http://hdl.handle.net/2434/580991.
Повний текст джерела