Статті в журналах з теми "Fleming`s model"

This study examines the fiscal and monetary policy in Indonesia using the Mundell-Fleming model. The main objective of this study was to determine which policies are effective between fiscal and monetary policies of the national income in Indonesia because Indonesia is a small open economy with not perfect capital mobility. The analysis technique used is Two Stage Least Square (TSLS) by using secondary data base on International Financial Statistics, 2000.I 2014.II . The research result is monetary policy is more effective than the fiscal policy in which monetary policy multiplier at 0.0028 greater than fiscal policy multiplier 0.001316. The results are consistent with the theory of the Mundell-Fleming.

Genetic Algorithms (GAs) are an adaptive heuristic search algorithm premised on the evolutionary ideas of natural selection and genetic. In this study we apply GAs for Fundamental Models of Exchange Rate Determination in exchange rate market. In this framework, we estimated absolute and relative purchasing power parity, Mundell-Fleming, sticky and flexible prices, equilibrium exchange rate and portfolio balance model as fundamental models for European Union’s Euro against the US Dollar using monthly data from January 1992 to December 2008. Then, we put these models into the genetic algorithm system for measuring their optimal weight for each model. These optimal weights have been measured according to four criteria i.e. R-squared (R2), mean square error (MSE), mean absolute percentage error (MAPE) and root mean square error (RMSE). Based on obtained Results, it seems that for explaining of EU Euro against the US Dollar exchange rate behavior, equilibrium exchange rate and portfolio balance model are better than the other fundamental models.

Abstract A thermodynamic model is presented for modeling the partitioning of amphiphilic species between the different partitioning of amphiphilic species between the different phases of systems typically used for chemical flooding. phases of systems typically used for chemical flooding. The model, an extension of the pseudophase model by Biais et al. that can analyze only a four-component system, can work with five-component systems, including two partitioning amphiphilic species (e.g., two alcohols or one alcohol and a partitioning cosurfactant species). The self-association of alcohol in the organic phases, which results in a variable alcohol partition coefficient, is considered. Experiments to determine thermodynamic constants (which are entered into the model) are described for four-component systems, including one alcohol. The salinity dependence of these parameters is also studied. Brine/decane/isobutanol/TRS 10–410 as well as brine/nonane/ isopropanol/TRS 10–80 systems are considered. Some computations of pseudophase compositions for the five-component model and for various overall compositions are included. This partitioning model has been included in the chemical-flooding simulator developed at the U. of Texas; the results of this model have been presented in another paper. The model used for the presented in another paper. The model used for the binodal surface that is required to calculate phase compositions from pseudophase compositions is presented in this paper, as well as comparisons with experimental data for both four- and five-component systems. Reservoir simulation results are presented in Ref. 3. Introduction The possibility of reaching very low interfacial tensions (IFT) during the displacement of oil by surfactant solutions has been the subject of intense interest for some time. Because the decrease in IFT can be as much as several orders of magnitude, almost all the contacted oil can be mobilized by this process. However, the recovery rate has proved to be very sensitive to many parameters, and the process has to be designed carefully to achieve a good oil recovery. It is commonly recognized that the phase behavior is one of the most critical features for the phase behavior is one of the most critical features for the design of chemical oil-recovery processes. Many investigators have studied phase behavior of systems with various combinations of brine, oil, surfactants, and cosurfactants. Winsor introduced a very convenient classification of phase behavior for such systems. Type I is a lower-phase microemulsion (surfactant-rich phase) in equilibrium with an oleic phase; Type II is an phase) in equilibrium with an oleic phase; Type II is an upper-phase microemulsion in equilibrium with an aqueous phase, and Type III corresponds to a middle-phase microemulsion in equilibrium with both aqueous lower phase and oleic upper phase. The number of phases and their composition determined IFT's, viscosity, relative permeabilities and other hydrodynamic parameters on permeabilities and other hydrodynamic parameters on which the efficiency of the process is directly dependent. Components present in the reservoir during chemical flooding include water, electrolytes, oil, polymer, and the amphiphilic species surfactant and cosurfactant. From the viewpoint of chemical thermodynamics, the number of chemical species is very large if we consider every species of which oil, surfactant, and cosurfactant are made. Fortunately, some of these species behave collectively, so they can be considered a single pseudocomponent in the phase behavior description, thereby pseudocomponent in the phase behavior description, thereby making the study more tractable. For example, Vinatieri and Fleming considered brine a good pseudocomponent, which means that the ratio of salt to water is about the same in each phase. McQuigg et al.'s experiments yield similar conclusions. Even crude oil has been shown to be a good pseudocomponent with a fairly acceptable accuracy. Dealing with amphiphilic species is far more difficult. In some laboratory studies, surfactant can be a chemically pure component, but for field applications it is usually a complex blend, such as petroleum sulfonates. In the case of petroleum sulfonates, different monosulfonated or polysulfonated species are present with varied carbon polysulfonated species are present with varied carbon tails. Commercial nonionic surfactants, which generally are ethoxylated alcohols, show a broad distribution of ethylene oxide number (EON). In both cases, investigators have shown that these commercially available surfactants do not behave collectively but in some situations partition selectively between the phases. The cosurfactant generally is an alcohol or an ethoxylated alcohol. Although many research programs currently are devoted to the design of alcohol-free systems to avoid some of the drawbacks induced by its presence (lower solubilization parameters, higher IFT's), most of the commonly used systems include alcohol or even a blend of alcohols with different carbon chain lengths and/or branching. SPEJ P. 693

There are three different species of redfish (Sebastes spp.) in the waters of the Flemish Cap (Division 3M, NAFO Regulatory Area): S. fasciatus, S. mentella, and S. norvegicus. Historically, S. fasciatus and S. mentella have been managed together as a single stock because of similar biology and difficulty in species identification. Here we use multivariate autoregressive state-space models to examine the abundance trajectories of the three species and to determine whether they can be treated as a single stock for management purposes or whether they should be treaty separately. We also included covariates to evaluate relationships with climate, commercial catch, and the abundance of predators and (or) competitors and prey. We did two separate analyses: (i) a single-period analysis over the full time series and (ii) a blocked, two-period analysis over different regulatory periods. In both analyses, the best-fit model included separate trajectories for each species at each depth but one overall stock growth rate; both also included commercial catches as a covariate. These analyses suggest that a single assessment for the Sebastes complex is acceptable.

Abstract The Prose Poem in Flanders and the Netherlands as a Model. On Genres and GenerationsThis article explores the ways in which the genre of the prose poem functioned as a model in Flemish and Dutch literature from the 1890’s to the 1920’s. Focusing on two cases ‐ a prewar and a postwar one ‐, it is argued that the genre was reinterpreted by new literary generations and, infused with new elements, became a productive model for new texts. A first case study deals with Pol de Mont and Ellen Corr. The former modified the model as he borrowed it from the authors of the Eighties movement, and it subsequently became a productive model for an epigone like Corr. In a second case study, dealing with Herman Heijermans and Constant van Wessem, we show how the genre was modified again in the period following World War I, and was made to incorporate modern elements such as cinematic processes and a businesslike, objective form. Finally, these cases reveal that models go beyond the genre, since their evolution is often based on eye-catching features that are not necessarily essential to the definition of the genre per se.

In navolging van zijn oud-leraren en medebroeders Jozef Van Mierlo junior en Desideer Stracke, ontpopte de jezuïetenpater Jozef Van Opdenbosch zich tot een vooraanstaande propagandist van het etnisch-culturele Vlaams-nationalisme in het interbellum.In de late jaren 1920 werd Van Opdenbosch een van de voornaamste inspiratoren van het Algemeen Katholiek Vlaamsch Studentenverbond (AKVS).In de jaren 1930 publiceerde hij artikels en brochures waarin hij de zelfstandige katholieke Vlaamse studentenbeweging verdedigde tegen de door het episcopaat geleide Katholieke Studentenactie (KSA). Tegelijk pleitte hij in die publicaties voor de vernietiging van het tegennatuurlijke, liberale België, en voor de stichting van een organisch ingerichte, op katholieke leest geschoeide Groot-Nederlandse volksstaat.________A figure head of extreme-right Catholic Flemish nationalism during the interbellum period: Jozef Van Opdenbosch S.J. (1892-1944)Following the example of his former teachers and fellow brothers Jozef Van Mierlo Junior and Desideer Stracke the Jesuit Father Jozef Van Opdenbosch turned out to be a prominent propagandist of the ethnico-cultural Flemish nationalism during the interbellum period. At the end of the 1920's Van Opdenbosch became one of the main sources of inspiration of the General Catholic Flemish Student Union (AKVS).During the 1930's he published articles and leaflets in which he defended the independent Catholic Flemish Student Union against the Catholic Students' Action (KSA) which was run by the diocese. In those publications he also argued for the destruction of the unnatural liberal state of Belgium, and in favour of the foundation of an organically set up Greater Netherlands nation, based on a Catholic model.

Microstructures and mechanical properties of directionally solidified Mg-xGd (5.21, 7.96 and 9.58 wt.%) alloys were investigated at a wide range of growth rates (V = 10-200 μm/s) under the constant temperature gradient (G = 30 K/mm). The results showed that when the growth rate was 10 μm/s, different interface morphologies were observed in three tested alloys: cellular morphology for Mg-5.21Gd alloy, a mixed morphology of cellular structure and dendritic structure for Mg-7.96Gd alloy and dendrite morphology for Mg-9.58Gd alloy, respectively. Upon further increasing the growth rate, only dendrite morphology was exhibited in all experimental alloys. The microstructural parameters (λ1, λ2) decreased with increasing the growth rate for all the experimental alloy, and the measured λ1 and λ2 values were in good agreement with Trivedi model and Kattamis-Flemings model, respectively. Vickers hardness and the ultimate tensile strength increased with the increase of the growth rate and Gd content, while the elongation decreased gradually. Furthermore, the relationships between the hardness, ultimate tensile strength, the growth rate and the microstructural parameters were discussed and compared with the previous experimental results.

This paper presents a comparative study which investigates the influence of Saudi Arabic guttural consonants /χ/, /ħ/ and /h/ on the vowel /a/ when they are adjacent and in the same syllable. Cohn (2007, 2009), Flemming (2001), and Keating (1996) discuss a unified model in which phonology and phonetics are treated as two distinct elements of one domain where each element has an effect on the other to some degree. McCarthy (1991, 1994), Rose (1996), Zawaydeh (1999, 2004), and BinMuqbil (2006) presented phonological studies on gutturals, as well as discussions on gutturals as a natural class, which uphold the phonological aspect of Cohn’s (2009) unified model. The aim of this study is to address the phonetic aspect of Cohn’s (2009) unified model by analyzing the phonetic effects of guttural-vowel coarticulation. An acoustic analysis method was used as a framework for this investigation to extract first formant frequency (F1) and second formant frequency (F2) to measure the influence in the coarticulation. For the purpose of this study, seven native Saudi Arabic speakers were recorded pronouncing 70 Saudi Arabic words. The results showed that guttural consonants have an influence on the vowel /a/ by lowering and backing it when they are adjacent and in the same syllable, while the vowel /a/ in the nonguttural consonants is raising and fronting their adjacent vowel /a/ in the same syllable in comparison with the vowel /a/ in the guttural environment.

AbstractBenthic storms are important for both the energy budget of the ocean and for sediment resuspension and transport. Using 30 years of output from a high-resolution model of the North Atlantic, it is found that most of the benthic storms in the model occur near the western boundary in association with the Gulf Stream and the North Atlantic Current, in regions that are generally collocated with the peak near-bottom eddy kinetic energy. A common feature is meander troughs in the near-surface jets that are accompanied by deep low pressure anomalies spinning up deep cyclones with near-bottom velocities of up to more than 0.5 m s−1. A case study of one of these events shows the importance of both baroclinic and barotropic instability of the jet, with energy being extracted from the jet in the upstream part of the meander trough and partly returned to the jet in the downstream part of the meander trough. This motivates examining the 30-yr time mean of the energy transfer from the (annual mean) background flow into the eddy kinetic energy. This quantity is shown to be collocated well with the region in which benthic storms and large increases in deep cyclonic relative vorticity occur most frequently, suggesting an important role for mixed barotropic–baroclinic instability-driven cyclogenesis in generating benthic storms throughout the model simulation. Regions of the largest energy transfer and most frequent benthic storms are found to be the Gulf Stream west of the New England Seamounts and the North Atlantic Current near Flemish Cap.

Abstract The mechanisms involved in setting the annual cycle of the Florida Current transport are revisited using an adjoint model approach. Adjoint sensitivities of the Florida Current transport to wind stress reproduce a realistic seasonal cycle with an amplitude of ~1.2 Sv (1 Sv ≡ 106 m3 s−1). The annual cycle is predominantly determined by wind stress forcing and related coastal upwelling (downwelling) north of the Florida Strait along the shelf off the North American coast. Fast barotropic waves propagate these anomalies southward and reach the Florida Strait within a month, causing an amplitude of ~1 Sv. Long baroclinic planetary Rossby waves originating from the interior are responsible for an amplitude of ~0.8 Sv but have a different phase. The sensitivities corresponding to the first baroclinic mode propagate westward and are highly influenced by topography. Considerable sensitivities are only found west of the Mid-Atlantic Ridge, with maximum values at the western shelf edge. The second baroclinic mode also has an impact on the Florida Current variability, but only when a mean flow is present. A second-mode wave train propagates southwestward from the ocean bottom on the western side of the Mid-Atlantic Ridge between ~36° and 46°N and at Flemish Cap, where the mean flow interacts with topography, to the surface. Other processes such as baroclinic waves along the shelf and local forcing within the Florida Strait are of minor importance.

Background: Learning styles are inherent personal attributes that determine the preferred teaching and learning method(s) for individual while an intelligence type is an individual’s levels of aptitude in various content areas as there is no one individual who is universally intelligent. An understanding of the two concepts is essential for designing suitable teaching approaches.Methods: This was a descriptive correlation study which explored the relationship between two variables; learning styles and intelligence types and academic performance of nursing students enrolled in the conventional nursing program at the University of Zambia. Data on learning styles was collected using the Visual, Auditory, Reading/Writing and Kinesthetic (VARK) questionnaire version 7.8 as proposed by Neil Fleming while Gardner’s questionnaire for assessing multiple intelligences was used to collect data on the students’ intelligence types. Results for the end of 2015/2016 academic year were used to determine academic performance.Results: Almost two thirds 64 (65.4%) of the participants’ academic performance was at credit level, 32 (32.7%) were at pass while only 2 (2%) were meritorious students. Participants had wide-ranging learning styles from unimodal, to bimodal and tri modal styles, although the majority were unimodal learners 81 (82.6%). Among the unimodal learners, majority 35 (35.5%) were kinaesthetic. On the other hand, 37 (37.8%) had logical mathematical intelligence, followed by bodily-Kinaesthetic 27 (27.6%). Both learning styles and intelligence types showed negative correlation and no significant association with academic performance. The Pearson r correlation and level of significance between learning styles and academic performance was -.092 and 0.372 respectively, while that for intelligence types and academic performance were -.027 and 0.790. Of the three demographic variables of age, gender and year of study, only age showed a positive correlation and significant association with academic performance (p = .002, and r = .144).Conclusions: The negative correlation between learning styles and intelligence types and academic performance may be an indication that the teaching methods utilized for nursing students are varied and therefore capable of promoting learning across different styles and intelligence types without necessarily favouring a specific style or type or that there is simply no association between learning styles and intelligence types and academic performance.

Abstract STUDY QUESTION What is the role of POU class 5 homeobox 1 (POU5F1) in human preimplantation development and how does it compare with the mouse model? SUMMARY ANSWER POU5F1 is required for successful development of mouse and human embryos to the blastocyst stage as knockout embryos exhibited a significantly lower blastocyst formation rate, accompanied by lack of inner cell mass (ICM) formation. WHAT IS KNOWN ALREADY Clustered regularly interspaced short palindromic repeats—CRISPR associated genes (CRISPR-Cas9) has previously been used to examine the role of POU5F1 during human preimplantation development. The reported POU5F1-targeted blastocysts always retained POU5F1 expression in at least one cell, because of incomplete CRISPR-Cas9 editing. The question remains of whether the inability to obtain fully edited POU5F1-targeted blastocysts in human results from incomplete editing or the actual inability of these embryos to reach the blastocyst stage. STUDY DESIGN, SIZE, DURATION The efficiency of CRISPR-Cas9 to induce targeted gene mutations was first optimized in the mouse model. Two CRISPR-Cas9 delivery methods were compared in the B6D2F1 strain: S-phase injection (zygote stage) (n = 135) versus metaphase II-phase (M-phase) injection (oocyte stage) (n = 23). Four control groups were included: non-injected media-control zygotes (n = 43)/oocytes (n = 48); sham-injected zygotes (n = 45)/oocytes (n = 47); Cas9-protein injected zygotes (n = 23); and Cas9 protein and scrambled guide RNA (gRNA)-injected zygotes (n = 27). Immunofluorescence analysis was performed in Pou5f1-targeted zygotes (n = 37), media control zygotes (n = 19), and sham-injected zygotes (n = 15). To assess the capacity of Pou5f1-null embryos to develop further in vitro, additional groups of Pou5f1-targeted zygotes (n = 29) and media control zygotes (n = 30) were cultured to postimplantation stages (8.5 dpf). Aiming to identify differences in developmental capacity of Pou5f1-null embryos attributed to strain variation, zygotes from a second mouse strain—B6CBA (n = 52) were targeted. Overall, the optimized methodology was applied in human oocytes following IVM (metaphase II stage) (n = 101). The control group consisted of intracytoplasmically sperm injected (ICSI) IVM oocytes (n = 33). Immunofluorescence analysis was performed in human CRISPR-injected (n = 10) and media control (n = 9) human embryos. PARTICIPANTS/MATERIALS, SETTING, METHODS A gRNA-Cas9 protein mixture targeting exon 2 of Pou5f1/POU5F1 was microinjected in mouse oocytes/zygotes or human IVM oocytes. Reconstructed embryos were cultured for 4 days (mouse) or 6.5 days (human) in sequential culture media. An additional group of mouse-targeted zygotes was cultured to postimplantation stages. Embryonic development was assessed daily, with detailed scoring at late blastocyst stage. Genomic editing was assessed by immunofluorescence analysis and next-generation sequencing. MAIN RESULTS AND THE ROLE OF CHANCE Genomic analysis in mouse revealed very high editing efficiencies with 95% of the S-Phase and 100% of the M-Phase embryos containing genetic modifications, of which 89.47% in the S-Phase and 84.21% in the M-Phase group were fully edited. The developmental capacity was significantly compromised as only 46.88% embryos in the S-Phase and 19.05% in the M-Phase group reached the blastocyst stage, compared to 86.36% in control M-Phase and 90.24% in control S-Phase groups, respectively. Immunofluorescence analysis confirmed the loss of Pou5f1 expression and downregulation of the primitive marker SRY-Box transcription factor (Sox17). Our experiments confirmed the requirement of Pou5f1 expression for blastocyst development in the second B6CBA strain. Altogether, our data obtained in mouse reveal that Pou5f1 expression is essential for development to the blastocyst stage. M-Phase injection in human IVM oocytes (n = 101) similarly resulted in 88.37% of the POU5F1-targeted embryos being successfully edited. The developmental capacity of generated embryos was compromised from the eight-cell stage onwards. Only 4.55% of the microinjected embryos reached the late blastocyst stage and the embryos exhibited complete absence of ICM and an irregular trophectoderm cell layer. Loss of POU5F1 expression resulted in absence of SOX17 expression, as in mouse. Interestingly, genetic mosaicism was eliminated in a subset of targeted human embryos (9 out of 38), three of which developed into blastocysts. LIMITATIONS, REASONS FOR CAUTION One of the major hurdles of CRISPR-Cas9 germline genome editing is the occurrence of mosaicism, which may complicate phenotypic analysis and interpretation of developmental behavior of the injected embryos. Furthermore, in this study, spare IVM human oocytes were used, which may not recapitulate the developmental behavior of in vivo matured oocytes. WIDER IMPLICATIONS OF THE FINDINGS Comparison of developmental competency following CRISPR-Cas-mediated gene targeting in mouse and human may be influenced by the selected mouse strain. Gene targeting by CRISPR-Cas9 is subject to variable targeting efficiencies. Therefore, striving to reduce mosaicism can provide novel molecular insights into mouse and human embryogenesis. STUDY FUNDING/COMPETING INTEREST(S) The research was funded by the Ghent University Hospital and Ghent University and supported by the FWO-Vlaanderen (Flemish fund for scientific research, Grant no. G051516N), and Hercules funding (FWO.HMZ.2016.00.02.01). The authors declare no competing interests. TRIAL REGISTRATION NUMBER N/A.

Abstract STUDY QUESTION Can we use a mitochondrial-targeted antioxidant (Mitoquinone) during in vitro embryo culture to rescue developmental competence of oocytes matured under lipotoxic conditions, exhibiting mitochondrial dysfunction and oxidative stress? SUMMARY ANSWER Supplementation of embryo culture media with Mitoquinone reduced oxidative stress and prevented mitochondrial uncoupling in embryos derived from metabolically compromised oocytes in vitro, leading to higher blastocyst rates and lower blastomeric apoptosis. WHAT IS KNOWN ALREADY Maternal metabolic disorders, such as obesity and type-II diabetes are associated with hyperlipidemia and elevated free fatty acid (FFA) concentrations in the ovarian follicular fluid (FF). Oocyte maturation under these lipotoxic conditions results in increased oxidative stress levels, mitochondrial dysfunction, reduced developmental competence and disappointing IVF results. STUDY DESIGN, SIZE, DURATION A well-described bovine oocyte IVM model was used, where a pathophysiologically relevant elevated FF concentrations of palmitic acid (PA; 150 μM or 300 μM) were added to induce oxidative stress. After fertilization (Day 0, D0), zygotes were in vitro cultured (IVC, from D1 to D8) in standard fatty acid-free media in the presence or absence of Mitoquinone or its carrier triphenyl-phosphonium. PARTICIPANTS/MATERIALS, SETTING, METHODS Embryo cleavage and fragmentation (D2) and blastocyst rates (D8) were recorded. Mitochondrial activity and oxidative stress in cleaved embryos at D2 were determined using specific fluorogenic probes and confocal microscopy. D8 blastocysts were used to (i) examine the expression of marker genes related to mitochondrial unfolded protein responses (UPRmt; HSPD1 and HSPE1), mitochondrial biogenesis (TFAM), endoplasmic reticulum (ER) UPR (ATF4, ATF6 and BiP) and oxidative stress (CAT, GPX1 and SOD2) using real time RT-PCR; (ii) determine cell differentiation and apoptosis using CDX-2 and cleaved caspase-3 immunostaining; and (iii) measure mtDNA copy numbers. This was tested in a series of experiments with at least three independent replicates for each, using a total of 2525 oocytes. Differences were considered significant if a P value was <0.05 after Bonferroni correction. MAIN RESULTS AND THE ROLE OF CHANCE Exposure to PA during IVM followed by culture under control conditions resulted in a significant increase in oxidative stress in embryos at D2. This was associated with a significant reduction in mitochondrial inner membrane potential (uncoupling) compared with solvent control (P < 0.05). The magnitude of these effects was PA-concentration dependent. Consequently, development to the blastocysts stage was significantly hampered. Surviving blastocysts exhibited high apoptotic cell indices and upregulated mRNA expression indicating persistent oxidative stress, mitochondrial and ER UPRs. In contrast, supplementation of PA-derived zygotes with Mitoquinone during IVC (i) prevented mitochondrial uncoupling and alleviated oxidative stress at D2; and (ii) rescued blastocyst quality; normalized oxidative stress and UPR related genes and apoptotic cell indices (P > 0.01 compared with solvent control). Mitoquinone also improved blastocyst rate in PA-exposed groups, an effect that was dependent on PA concentration. LARGE SCALE DATA N/A LIMITATIONS, REASONS FOR CAUTION This is a fundamental study performed using a bovine in vitro model using PA-induced lipotoxicity during oocyte maturation. PA is the most predominant FFA in the FF that is known to induce lipotoxicity; however, in vivo maturation in patients suffering from maternal metabolic disorders involve more factors that cannot be represented in one model. Nevertheless, focusing on the carryover oxidative stress as a known key factor affecting developmental competence, and considering the novel beneficial rescuing effects of Mitoquinone shown here, we believe this model is of high biological relevance. WIDER IMPLICATIONS OF THE FINDINGS Human oocytes collected for IVF treatments from patients with maternal metabolic disorders are vulnerable to lipotoxicity and oxidative stress during in vivo maturation. The results shown here suggest that mitochondrial targeted therapy, such as using Mitoquinone, during IVC may rescue the developmental competence and quality of these compromised oocytes. After further clinical trials, this may be a valuable approach to increase IVF success rates for infertile patients experiencing metabolic disorders. STUDY FUNDING/COMPETING INTEREST(S) This study was financially supported by a BOF/KP grant number 34399, from the University of Antwerp, Belgium. W.F.A.M. was supported by a postdoctoral fellowship from the Research Foundation-Flanders (FWO), grant number 12I1417N, Antwerp, Belgium. The Leica SP 8 confocal microscope used in this study was funded by the Hercules Foundation of the Flemish Government (Hercules grant AUHA.15.12). All authors have no financial or non-financial competing interests to declare.

Abstract STUDY QUESTION Does oocyte maturation under lipolytic conditions have detrimental carry-over effects on post-hatching embryo development of good-quality blastocysts after transfer? SUMMARY ANSWER Surviving, morphologically normal blastocysts derived from bovine oocytes that matured under lipotoxic conditions exhibit long-lasting cellular dysfunction at the transcriptomic and metabolic levels, which coincides with retarded post-hatching embryo development. WHAT IS KNOWN ALREADY There is increasing evidence showing that following maturation in pathophysiologically relevant lipotoxic conditions (as in obesity or metabolic syndrome), surviving blastocysts of good (transferable) morphological quality have persistent transcriptomic and epigenetic alteration even when in vitro embryo culture takes place under standard conditions. However, very little is known about subsequent development in the uterus after transfer. STUDY DESIGN, SIZE, DURATION Bovine oocytes were matured in vitro in the presence of pathophysiologically relevant, high non-esterified fatty acid (NEFA) concentrations (HIGH PA), or in basal NEFA concentrations (BASAL) as a physiological control. Eight healthy multiparous non-lactating Holstein cows were used for embryo transfers. Good-quality blastocysts (pools of eight) were transferred per cow, and cows were crossed over for treatments in the next replicate. Embryos were recovered 7 days later and assessed for post-hatching development, phenotypic features and gene expression profile. Blastocysts from solvent-free and NEFA-free maturation (CONTROL) were also tested for comparison. PARTICIPANTS/MATERIALS, SETTING, METHODS Recovered Day 14 embryos were morphologically assessed and dissected into embryonic disk (ED) and extraembryonic tissue (EXT). Samples of EXT were cultured for 24 h to assess cellular metabolic activity (glucose and pyruvate consumption and lactate production) and embryos’ ability to signal for maternal recognition of pregnancy (interferon-τ secretion; IFN-τ). ED and EXT samples were subjected to RNA sequencing to evaluate the genome-wide transcriptome patterns. MAIN RESULTS AND THE ROLE OF CHANCE The embryo recovery rate at Day 14 p.i. was not significantly different among treatment groups (P &gt; 0.1). However, higher proportions of HIGH PA embryos were retarded in growth (in spherical stage) compared to the more elongated tubular stage embryos in the BASAL group (P &lt; 0.05). Focusing on the normally developed tubular embryos in both groups, HIGH PA exposure resulted in altered cellular metabolism and altered transcriptome profile particularly in pathways related to redox-regulating mechanisms, apoptosis, cellular growth, interaction and differentiation, energy metabolism and epigenetic mechanisms, compared to BASAL embryos. Maturation under BASAL conditions did not have any significant effects on post-hatching development and cellular functions compared to CONTROL. LARGE-SCALE DATA The datasets of RNA sequencing analysis are available in the NCBI’s Gene Expression Omnibus (GEO) repository, series accession number GSE127889 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE127889). Datasets of differentially expressed genes and their gene ontology functions are available in the Mendeley datasets at http://dx.doi.org/10.17632/my2z7dvk9j.2. LIMITATIONS, REASONS FOR CAUTION The bovine model was used here to allow non-invasive embryo transfer and post-hatching recovery on Day 14. There are physiological differences in some characteristics of post-hatching embryo development between human and cows, such as embryo elongation and trophoblastic invasion. However, the main carry-over effects of oocyte maturation under lipolytic conditions described here are evident at the cellular level and therefore may also occur during post-hatching development in other species including humans. In addition, post-hatching development was studied here under a healthy uterine environment to focus on carry-over effects originating from the oocyte, whereas additional detrimental effects may be induced by maternal metabolic disorders due to adverse changes in the uterine microenvironment. RNA sequencing results were not verified by qPCR, and no solvent control was included. WIDER IMPLICATIONS OF THE FINDINGS Our observations may increase the awareness of the importance of maternal metabolic stress at the level of the preovulatory oocyte in relation to carry-over effects that may persist in the transferrable embryos. It should further stimulate new research about preventive and protective strategies to optimize maternal metabolic health around conception to maximize embryo viability and thus fertility outcome. STUDY FUNDING/COMPETING INTEREST(S) This study was supported by the Flemish Research Fund (FWO grant 11L8716N and FWO project 42/FAO10300/6541). The authors declare there are no conflicts of interest.

Abstract STUDY QUESTION Can pronuclear transfer (PNT) or maternal spindle transfer (ST) be applied to overcome poor embryo development associated with advanced maternal age or early embryo arrest in a mouse model? SUMMARY ANSWER Both PNT and ST may have the potential to restore embryonic developmental potential in a mouse model of reproductive ageing and embryonic developmental arrest. WHAT IS KNOWN ALREADY Germline nuclear transfer (NT) techniques, such as PNT and ST, are currently being applied in humans to prevent the transmission of mitochondrial diseases. Yet, there is also growing interest in the translational use of NT for treating infertility and improving IVF outcomes. Nevertheless, direct scientific evidence to support such applications is currently lacking. Moreover, it remains unclear which infertility indications may benefit from these novel assisted reproductive technologies. STUDY DESIGN, SIZE, DURATION We applied two mouse models to investigate the potential of germline NT for overcoming infertility. Firstly, we used a model of female reproductive ageing (B6D2F1 mice, n = 155), with ages ranging from 6 to 8 weeks (young), 56 (aged) to 70 weeks (very-aged), corresponding to a maternal age of &lt;30, ∼36 and ∼45 years in humans, respectively. Secondly, we used NZB/OlaHsd female mice (7–14 weeks, n = 107), as a model of early embryo arrest. This mouse strain exhibits a high degree of two-cell block. Metaphase II (MII) oocytes and zygotes were retrieved following superovulation. PARTICIPANTS/MATERIALS, SETTING, METHODS Ovarian reserve was assessed by histological analysis in the reproductive-aged mice. Mitochondrial membrane potential (△Ψm) was measured by JC-1 staining in MII oocytes, while spindle-chromosomal morphology was examined by confocal microscopy. Reciprocal ST and PNT were performed by transferring the meiotic spindle or pronuclei (PN) from unfertilised or fertilised oocytes (after ICSI) to enucleated oocytes or zygotes between aged or very-aged and young mice. Similarly, NT was also conducted between NZB/OlaHsd (embryo arrest) and B6D2F1 (non-arrest control) mice. Finally, the effect of cytoplasmic transfer (CT) was examined by injecting a small volume (∼5%) of cytoplasm from the oocytes/zygotes of young (B6D2F1) mice to the oocytes/zygotes of aged or very-aged mice or embryo-arrest mice. Overall, embryonic developmental rates of the reconstituted PNT (n = 572), ST (n = 633) and CT (n = 336) embryos were assessed to evaluate the efficiency of these techniques. Finally, chromosomal profiles of individual NT-generated blastocysts were evaluated using next generation sequencing. MAIN RESULTS AND THE ROLE OF CHANCE Compared to young mice, the ovarian reserve in aged and very-aged mice was severely diminished, reflected by a lower number of ovarian follicles and a reduced number of ovulated oocytes (P &lt; 0.001). Furthermore, we reveal that the average △Ψm in both aged and very-aged mouse oocytes was significantly reduced compared to young mouse oocytes (P &lt; 0.001). In contrast, the average △Ψm in ST-reconstructed oocytes (very-aged spindle and young cytoplast) was improved in comparison to very-aged mouse oocytes (P &lt; 0.001). In addition, MII oocytes from aged and very-aged mice exhibited a higher rate of abnormalities in spindle assembly (P &lt; 0.05), and significantly lower fertilisation (60.7% and 45.3%) and blastocyst formation rates (51.4% and 38.5%) following ICSI compared to young mouse oocytes (89.7% and 87.3%) (P &lt; 0.001). Remarkably, PNT from zygotes obtained from aged or very-aged mice to young counterparts significantly improved blastocyst formation rates (74.6% and 69.2%, respectively) (P &lt; 0.05). Similarly, both fertilisation and blastocyst rates were significantly increased after ST between aged and young mice followed by ICSI (P &lt; 0.05). However, we observed no improvement in embryo development rates when performing ST from very-aged to young mouse oocytes following ICSI (P &gt; 0.05). In the second series of experiments, we primarily confirmed that the majority (61.8%) of in vivo zygotes obtained from NZB/OlaHsd mice displayed two-cell block during in vitro culture, coinciding with a significantly reduced blastocyst formation rate compared to the B6D2F1 mice (13.5% vs. 90.7%; P &lt; 0.001). Notably, following the transfer of PN from the embryo-arrest (NZB/OlaHsd) zygotes to enucleated non-arrest (B6D2F1) counterparts, most reconstructed zygotes developed beyond the two-cell stage, leading to a significantly increased blastocyst formation rate (89.7%) (P &lt; 0.001). Similar findings were obtained after implementing ST between NZB/OlaHsd and B6D2F1 mice, followed by ICSI. Conversely, the use of CT did not improve embryo development in reproductive-age mice nor in the embryo-arrest mouse model (P &gt; 0.05). Surprisingly, chromosomal analysis revealed that euploidy rates in PNT and ST blastocysts generated following the transfer of very-aged PN to young cytoplasts and very-aged spindles to young cytoplasts were comparable to ICSI controls (with young mouse oocytes). A high euploidy rate was also observed in the blastocysts obtained from either PNT or ST between young mice. Conversely, the transfer of young PN and young spindles into very-aged cytoplasts led to a higher rate of chromosomal abnormalities in both PNT and ST blastocysts. LARGE SCALE DATA N/A LIMITATIONS, REASONS FOR CAUTION The limited number of blastocysts analysed warrants careful interpretation. Furthermore, our observations should be cautiously extrapolated to humans given the inherent differences between mice and women in regards to various biological processes, including centrosome inheritance. The findings suggest that ST or PNT procedures may be able to avoid aneuploidies generated during embryo development, but they are not likely to correct aneuploidies already present in some aged MII oocytes. WIDER IMPLICATIONS OF THE FINDINGS To our knowledge, this is the first study to evaluate the potential of PNT and ST in the context of advanced maternal age and embryonic developmental arrest in a mouse model. Our data suggest that PNT, and to a lesser extent ST, may represent a novel reproductive strategy to restore embryo development for these indications. STUDY FUNDING/COMPETING INTEREST(S) M.T. is supported by grants from the China Scholarship Council (CSC) (Grant no. 201506160059) and the Special Research Fund from Ghent University (Bijzonder Onderzoeksfonds, BOF) (Grant no. 01SC2916 and no. 01SC9518). This research is also supported by the FWO-Vlaanderen (Flemish fund for scientific research, Grant no. G051017N, G051516N and G1507816N). The authors declare no competing interests. TRIAL REGISTRATION NUMBER N/A

In the Garden of EdenThe covers of the first three volumes of Kuml show photographs of fine Danish antiquities. Inside the volumes have articles on the Stone Age, the Bronze Age and the Iron Age in Jutland, which is to be expected as Kuml is published by the Jutland Archaeological Society. However, in 1954 the scene is moved to more southern skies. This year, the cover is dominated by a date palm with two huge burial mounds in the background. In side the book one reads no less than six articles on the results from the First Danish Archaeological Bahrain Expedition. P.V. Glob begins with: Bahrain – Island of the Hundred Thousand Burial Mounds, The Flint Sites of the Bahrain Desert, Temples at Barbar and The Ancient Capital of Bahrain, followed by Bibby’s Five among Bahrain’s Hundred Thousand Burial Mounds and The Well of the Bulls. The following years, reports on excavations on Bahrain and later in the sheikhdoms of Qatar, Kuwait and Abu Dhabi are on Kuml’s repertoire.However, it all ends wit h the festschrift to mark Glob’s 60th anniversary, Kuml 1970, which has three articles on Arab archaeology and a single article in 1972. For the past thirty years almost, the journal has not had a single article on Arabia. Why is that? Primarily because the character of the museum’s work in the Arabian Gulf changed completely. The pioneers’ years of large-scale reconnaissance and excavations were succeeded by labourous studies of the excavated material – the necessary work preceding the final publications. Only in Abu Dhabi and Oman, Karen Frifelt carried on the pioneer spirit through the 1970s and 1980s, but she mainly published her results in in ternational, Englishlanguage journals.Consequently, the immediate field reports ended, but the subsequent research into Arab archaeology – carried out at the writing desk and with the collections of finds– still crept into Kuml. From 1973 , the journal contained a list of the publications made by the Jutland Archaeological Society (abbreviated JASP), and here, the Arab monographs begin to make their entry. The first ones are Holger Kapel’s Atlas of the Stone Age Cultures of Qatar from 1967 and Geoffrey Bibby’s survey in eastern Saudi Arabia from 1973. Then comes the Hellenistic excavations on the Failaka island in Kuwait with Hans Erik Mathiesen’s treatise on the terracotta figurines (1982), Lise Hannestad’s work on the ceramics (1983) and Kristian Jeppesen’s presentation of the temple and the fortifications (1989). A similar series on the Bronze Age excavations on Failaka has started with Poul Kjærum’s first volume on the stamp and cylinder seals (1983) and Flemming Højlund’s presentation of the ceramics (1987). The excavations on the island of Umm an-Nar in Abu Dhabi was published by Karen Frifelt in two volumes on the settlement (1991) and the graves (1995), and the ancient capital of Bahrain was analysed by H. Hellmuth Andersen and Flemming Højlund in two volumes on the northern city wall and the Islamic fort (1994) and the central, monumental buildings (1997) respectively.More is on its way! A volume on Islamic finds made on Bahrain has just been made ready for printing, and the Bronze Age temples at the village of Barbar is being worked up. Danish and foreign scholars are preparing other volumes, but the most important results of the expeditions to the Arabian Gulf have by now been published in voluminous series.With this, an era has ended, and Moesgård Museum’s 50th anniversary in 1999 was a welcome opportunity of looking back at the Arabian Gulf effort through the exhibition Glob and the Garden ef Eden. The Danish Bahrain expeditions and to consider what will happen in the future.How then is the relation ship between Moesgård Museum and Bahrain today, twenty-three years after the last expedition – now that most of the old excavations have been published and the two originators of the expeditions, P.V. Glob and Geoffrey Bibby have both died?In Denmark we usually consider Bahrain an exotic country with an exciting past. However, in Bahrain there is a similar fascination of Denmark and of Moesgård Museum. The Bahrain people are wondering why Danish scholars have been interested in their small island for so many years. It was probably not a coincidence when in the 1980s archaeologist and ethnographers from Moesgård Museum were invited to take part in the furnishing of the exhibitions in the new national museum of Bahrain. Today, museum staff from Arab countries consider a trip to Moesgård a near-pilgrimage: our collection of Near East artefacts from all the Gulf countries is unique, and the ethnographic collections are unusual in that they were collected with thorough information on the use, the users and the origin of each item.The Bahrain fascination of Moesgård Museum. was also evident, when the Bahrain minister of education, Abdulaziz Al-Fadl, visited the museum in connection with the opening of the Bahrain exhibition in 1999.Al-Fadl visited the museum’s oriental department, and in the photo and film archive a book with photos taken by Danish members of the expeditions to the Arabian Gulf was handed over to him. Al-Fadl was absorbed by the photos of the Bahrain of his childhood – the 1950s and 1960s – an un spoilt society very different from the modern Bahrain. His enthusiasm was not lessened when he saw a photo of his father standing next to P.V. Glob and Sheikh Salman Al Khalifa taken at the opening of Glob’s first archaeological exhibition in Manama, the capital. At a banquet given by Elisabeth Gerner Nielsen, the Danish minister of culture, on the evening following the opening of the Glob exhibition at Moesgård, Al-Fadl revealed that as a child, he had been on a school trip to the Danish excavations where – on the edge of the excavation – he had his first lesson in Bahrain’s prehistory from a Danish archaeologist (fig. 1).Another example: When attending the opening of an art exhibition at Bahrain’s Art Centre in February 1999, I met an old Bahrain painter, Abdelkarim Al-Orrayed, who turned out to be a good friend of the Danish painter Karl Bovin, who took part in Glob’s expeditions. He told me, how in 1956, Bovin had exhibited his paintings in a school in Manama. He recalled Bovin sitting in his Arabian tunic in a corner of the room, playing a flute, which he had carved in Sheikh Ibrahim’s garden.In a letter, Al-Orrayed states: ”I remember very well the day in 1956, when I met Karl Bovin for the first time. He was drawin g some narrow roads in the residential area where I lived. I followed him closely with my friend Hussain As-Suni – we were twentythree and twenty-one years old respectively. When he had finished, I invited him to my house where I showed him my drawings. He looked at them closely and gave me good advice to follow if I wanted to become a skilful artist – such as focusing on lines, form, light, distance, and shadow. He encouraged me to practice outdoors and to use different models. It was a turning point in our young artists’ lives when Hussein and I decided to follow Bovin’s instructions. We went everywhere – to the teahouses, the markets, the streets, and the countryside – and practised there, but the sea was the most fascinating phenomenon to us. In my book, An Introduction to Modern Art in Bahrain, I wrote about Bovin’s exhibitions in the 1950s and his great influence on me as an artist. Bovin’s talent inspired us greatly in rediscovering the nature and landscape on Bahrain and gave us the feeling that we had much strength to invest in art. Bovin contributed to a new start to us young painters, who had chosen the nature as our main motif.”Abdelkarim Al-Orrayed was the first Bahrain painter to live of his art, and around 1960 he opened a studio from which he sold his paintings. Two of his landscape watercolours are now at Moesgård.These two stories may have revealed that Bahrain and Moesgard Museum have a common history, which both parts value and wish to continue. The mutual fascination is a good foundation to build on and the close bonds and personal acquaintance between by now more generations is a valuable counterbalance to those tendencies that estrange people, cultures, and countries from one another.Already, more joint projects have been initiated: Danish archaeology students are taking part in excavations on Bahrain and elsewhere in the Arabic Gulf; an ethnography student is planning a long stay in a village on Bahrain for the study of parents’ expectations to their children on Bahrain as compared with the conditions in Denmark; P.V. Glob’s book, Al-Bahrain, has been translated into Arabic; Moesgård’s photos and films from the Gulf are to become universally accessible via the Internet; an exhibition on the Danish expeditions is being prepared at the National Museum of Bahrain, and so forth.Two projects are to be described in more detail here: New excavations on Bahrain that are to investigate how fresh water was exploited in the past, and the publication of a book and three CDs, Music in Bahrain, which will make Bahrain’s traditional music accessible not just to the population of Bahrain, but to the whole world.New excavations on BahrainFor millennia, Bahrain was famous for its abundance of fresh water springs, which made a belt of oases across the northern half of the island possible. Natural fertility combined with the favourable situation in the middle of the Arab Gulf made Bahrain a cultural and commercial centre that traded with the cities of Mesopotamia and the IndusValley already in the third millennium BC.Fresh water also played an important part in Bahrain’s ancient religion, as seen from ar chaeological excavations and Mesopotamian cuneiform tablets: A magnificent temple of light limestone was built over a spring, and according to old texts, water was the gods’ gift to Bahrain (Dilmun).Although fresh water had an overwhelming importance to a parched desert island, no studies have been directed towards the original ”taming” of the water on Bahrain. Therefore, Moesgård Museum is now beginning to look into the earliest irrigation techniques on the island and their significance to Bahrain’s development.Near the Bahrain village of Barbar, P.V. Glob in 1954 discovered a rise in the landscape, which was excavated during the following years. It turned out that the mound covered three different temples, built on top of and around each other. The Barbar temple was built of whitish ashlars and must have been an impressive structure. It has also gained a special importance in Near East research, as this is the first and only time that the holy spring chamber, the abzu, where the god Enki lived, has been un earthed (fig. 2).On the western side of the Barbar temple a monumental flight of steps, flank ed on both sides by cult figures, was leading through a portal to an underground chamber with a fresh water spring. In the beautiful ashlar walls of this chamber were three openings, through which water flowed. Only the eastern out flow was investigated, as the outside of an underground stonebuilt aqueduct was found a few metres from the spring chamber.East of the temple another underground aqueduct was followed along a 16-m distance. It was excavated at two points and turned out almost to have the height of a man. The floor was covered with large stones with a carved canal and the ceiling was built of equally large stones (fig. 3).No doubt the spring chamber was a central part of the temple, charge d with great importance. However, the function of the aqueducts is still unknown. It seems obvious that they were to lead the fresh water away from the source chamber, but was this part of a completely ritual arrangement, or was the purpose to transport the water to the gardens to be used for irrigation?To clarify these questions we will try to trace the continuations of the aqueducts using different tracing techniques such as georadar and magnetometer. As the sur roundings of Barbar temple are covered by several metres of shifting sand, the possibilities of following the aqueducts are fine, if necessary even across a great distance, and if they turn out to lead to old gardens, then these may be exposed under the sand.Underground water canals of a similar construction, drawing water from springs or subsoil water, have been used until modern times on Bahrain, and they are still in use in Iran and on the Arabian Peninsula, especially in Oman, where they supply the gardens with water for irrigation. They are called qanats and are usually considered built by the Persians during periods when the Achaemenid or Sassanid kings controlled Arabia (c. 500 BC-c. 600 AD). However, new excavation results from the Oman peninsula indicate that at least some canal systems date from c. 1000 BC. It is therefore of utmost interest if similar sophisticated transportation systems for water on Bahrain may be proven to date from the time of the erection of the Barbar temple, i.e. c. 2000 BC.The finds suggest that around this time Bahrain underwent dramatic changes. From being a thinly inhabited island during most of the 3rd millennium BC, the northern part of the island suddenly had extensive burial grounds, showing a rapid increase in population. At the same time the major settlement on the northern coast was fortified, temples like the one at Barbar were built, and gigantic ”royal mounds” were built in the middle of the island – all pointing at a hierarchic society coming into existence.This fast social development of Dilmun must have parallelled efficiency in the exploitation of fresh water resources for farm ing to supply a growing population with the basic food, and perhaps this explains the aqueducts by Barbar?The planned excavatio ns will be carried out in close cooperation between the National Museum of Bahrain and Aarhus University, and they are supported financially by the Carlsberg Foundation and Bahrain’s Cabinet and Information Ministry.The music of BahrainThe composer Poul Rovsing Olsen (1922-1982) was inspired by Arab and Indian music, and he spent a large part of his life studying traditional music in the countries along the Arabian Gulf. In 1958 and 1962-63 he took part in P.V. Glob’s expeditions to Arabia as a music ethnologist and in the 1970s he organised stays of long duration here (fig. 4).The background for his musical fieldwork was the rapid development, which the oil finds in the Gulf countries had started. The local folk music would clearly disappear with the trades and traditions with which they were connected.” If no one goes pearl fishing anymore, then no one will need the work songs connected to this work. And if no one marries according to tradition with festivity lasting three or sometimes five days, then no one will need the old wedding songs anymore’’.It was thus in the last moment that Rovsing Olsen recorded the pearl fishers’ concerts, the seamen’s shanties, the bedouin war songs, the wedding music, the festival music etc. on his tape recorder. By doing this he saved a unique collection of song and music, which is now stored in the Dansk Folkemindesamling in Copenhagen. It comprises around 150 tapes and more than 700 pieces of music. The instruments are to be found at the Musikhistorisk Museum and Moesgård Museum (fig. 5).During the 1960s and 1970s Rovsing Olsen published a number of smaller studies on music from the Arabian Gulf, which established his name as the greatest connoisseur of music from this area – a reputation, which the twenty years that have passed since his death have not shaken. Rovsing Olsen also published an LP record with pearl fisher music, and with the music ethnologist Jean Jenkins from the Horniman Museum in London he published six LP records, Music in the World of Islam with seven numbers from the Arabian Gulf, and the book Music and Musical Instruments in the World of Islam (London 1976).Shortly before his death, Rovsing Olsen finished a comprehensive manuscript in English, Music in Bahrain, where he summed up nearly twenty-five years of studies into folk music along the Arabian Gulf, with the main emphasis on Bahrain. The manuscript has eleven chapters, and after a short introduction Rovsing Olsen deals with musical instruments, lute music, war and honour songs of the bedouins, festivity dance, working songs and concerts of the pearl fishers, music influenced front Africa, double clarinet and bag pipe music, religious songs and women’s songs. Of these, eighty-four selected pieces of music are reproduced with notes and commented in the text. A large selection of this music will be published on three CDs to go with the book.This work has been anticipated with great expectation by music ethnologists and connoisseurs of Arabic folk music, and in agreement with Rovsing Olsen’s widow, Louise Lerche-Lerchenborg and Dansk Folkemindesamling, Moesgård Museum is presently working on publishing the work.The publication is managed by the Jutland Archaeological Society and Aarhus University Press will manage the distribution. The Carlsberg Foundation and Bahrain’s Cabinet and Information Ministry will cover the editing and printing expenses.The publication of the book and the CDs on the music of Bahrain will be celebrated at a festivity on Bahrain, at the next annual cultural festival, the theme of which will be ”mutual inspiration across cultural borders” with a focus on Rovsing Olsen. In this context, Den Danske Trio Anette Slaato will perform A Dream in Violet, a music piece influenced by Arabic music. On the same occasion singers and musicians will present the traditional pearl fishers’ music from Bahrain. In connection with the concert on Bahrain, a major tour has been planned in cooperation with The Danish Institute in Damascus, where the Danish musicians will also perform in Damascus and Beirut and give ”masterclasses” in chamber music on the local music academies. The concert tour is being organised by Louise Lerche-Lerchenborg, who initiated one of the most important Danish musical events, the Lerchenborg Musical Days,in 1963 and organised them for thirty years.ConclusionPride of concerted effort is not a special Danish national sport. However,the achievements in the Arabian Gulf made by the Danish expeditions from the Århus museum are recognised everywhere. It is only fair to use this jubilee volume for drawing attention to the fact that the journal Kuml and the publications of the Jutland Archaeological Society were the instruments through which the epoch-making investigations in the Gulf were nude public nationally and internationally.Finally, the cooperationon interesting tasks between Moesgård Museum and the countries along the Arabian Gulf will continue. In the future, Kuml will again be reporting on new excavations in the palm shadows and eventually, larger investigation s will no doubt find their way to the society’s comprehensive volumes.Flemming HøjlundMoesgård MuseumTranslated by Annette Lerche Trolle

ABSTRACT Infections caused by multiple organisms, or polymicrobial infections, are likely more common than is broadly appreciated. Interaction among microbial communities (and with their host) can change the infection landscape by subverting immunity, providing nutrients and inhibiting competing microbes. Stacy et al. (A. Stacy, D. Fleming, R. J. Lamont, K. P. Rumbaugh, and M. Whiteley, mBio 7:e00782-16, 2016, http://dx.doi.org/10.1128/mBio.00782-16 ) described a novel mechanism that results in synergistic growth of oral microbes Aggregatibacter actinomycetemcomitans and Streptococcus gordonii . The authors used whole-genome fitness profiling by transposon sequencing (Tn-seq) to identify genes differentially required for growth in vitro versus in a mono- or coinfection in a thigh abscess model. They found that coinfection with S. gordonii allowed A. actinomycetemcomitans to shift from an anaerobic to an aerobic mode of growth. This shift involved the production of a terminal electron acceptor H 2 O 2 by S. gordonii and increased A. actinomycetemcomitans persistence—an interaction termed “cross-respiration.”

Abstract Background/Aims Fracture Liaison Services (FLSs) are recommended to deliver best practice in secondary fracture prevention. As part of the iFraP (Improving uptake of Fracture Prevention drug Treatments) research programme this study aimed to 1) co-design content for a ‘model FLS consultation’ and 2) gain consensus on the appropriateness of osteoporosis clinical guidelines in the context of FLSs. Methods Three rounds of modified Delphi survey were sent to patients with osteoporosis and/or fragility fractures, carers, and clinicians. Participants were presented with potential consultation content derived from an evidence synthesis of current guidelines, frameworks and theories of shared decision-making, communication and medicine adherence, and stakeholder consultation. Participants were asked to rate their perception of the importance of each statement on a 5-point Likert scale and elaborate using free-text boxes. In Round 2, participants were shown mean scores of importance from Round 1. Statements identified as of ‘low importance’ at the end of Rounds 1 and 2 were discussed by the study team, including patient contributors, and were removed or amended. In Round 3, participants were asked whether the statement was ‘essential’ or ‘optional’ in a time-limited FLS consultation. Percentage agreement with each statement was ranked. The threshold for ‘essential’ versus ‘optional’ was determined by the study team. Results 391 invitations to participate were sent, with 72, 49, and 52 responders to Rounds 1, 2 and 3 respectively. Throughout Rounds 1-3 participants considered 122 statements. By Round 3, 81 statements were deemed essential, with an additional 14 optional statements. Essential statements were distilled into 18 recommendations constituting the ‘model FLS consultation’. Statements related to stages of the consultation, including: introductions; gather information; consider therapeutic options; elicit patient perceptions; establish shared decision-making preferences; share information about condition and treatment; check understanding; and signpost next steps. There was consensus that FLS clinicians should discuss the benefits and risks of oral and intravenous bisphosphonates and denosumab. Optional consultation content included a statement suggesting clinicians should observe the patient to look for signs of fractures in their spine, with free-text responses suggesting that FLS clinicians may not ‘be best qualified’ to perform physical examinations. Removed statements included those relating to the discussion of Hormone Replacement Therapy, Raloxifene and Teriparatide, with free-text statements suggesting that ‘specialists’ (e.g. Rheumatologists) should discuss these medications. Additionally, statements that described the potential consequences of fracture (e.g. ‘the clinician should explain that one in ten patients with a hip fracture will die within 12 months of fracture.’) were removed. Free-text comments described these statements as potentially ‘scary’. Conclusion The Delphi survey has informed iFraP intervention development by highlighting essential and optional FLS consultation content. Findings also provide insight into aspects of current osteoporosis clinical guidelines deemed appropriate in nurse/allied health professional led FLSs. Disclosure L. Bullock: None. C. Jinks: Grants/research support; CJ is part funded by the NIHR Applied Research Collaboration (ARC) West Midlands. F. Crawford-Manning: Grants/research support; FCM is part funded by the NIHR Clinical Research Network Scholar Programme. S. Leyland: None. J. Fleming: None. E.M. Clark: None. E. Cottrell: None. J. Edwards: Grants/research support; JE is an NIHR Academic Clinical Lecturer in Primary Care (CL-2016-10-003). Z. Paskins: Grants/research support; ZP is funded by the NIHR, Clinician Scientist Award (CS-2018-18-ST2-010)/NIHR Academy.

Flemish Cap cod collapsed in the early 90’s after facing multiple threats due to climate variability and anthropogenic pressures. A master 35-year cod growth chronology was reconstructed in the present study by using sclerochronology techniques. Three additional chronologies were developed to account for the age-dependent effect of maturity and fishing intensity on growth. All chronologies showed that cod growth has decreased over the years and it is currently at the minimum level in the historic of data series. Hierarchical mixed-effects models were applied to the master chronology to identify extrinsic drivers of growth. The master chronology was divided into 3 periods: Premoratorium (1988-1998), Moratorium (1999-2009), and Postmoratorium (2010-2019). Our results showed that drivers of cod growth in the Flemish Cap varied between periods (Premoratorium: bottom temperature and fishing mortality, Moratorium: cod and pandalus abundance, and Postmoratorium: cod abundance and thickness of the cold intermediate layer). Our results suggest that density-dependence processes, food availability, and global warming may be behind the lack of recovery of the Flemish Cap cod stock. The Flemish Cap cod growth chronology shows variations on growth over time and identifies its drivers, having the potential to be included in the NAFO stock assessments or to be used to set dynamic reference points that guarantees the stock sustainability.

Abstract The Chandra X-ray Observatory has detected relatively hard X-ray emission from the central stars of several planetary nebulae (PNe). A subset has no known late-type companions, making it very difficult to isolate which of several competing mechanisms may be producing the X-ray emission. The central star of NGC 2392 is one of the most vexing members, with substantial indirect evidence for a hot white dwarf (WD) companion. Here we report on the results of a radial velocity (RV) monitoring campaign of its central star with the HERMES échelle spectrograph of the Flemish 1.2 m Mercator telescope. We discover a single-lined spectroscopic binary with an orbital period of 1.902208 ± 0.000013 d and an RV semi-amplitude of 9.96 ± 0.13 km s−1. The high degree of nebula ionisation requires a WD companion (M ≳ 0.6M⊙), which the mass-function supports at orbital inclinations ≲ 7°, in agreement with the nebula orientation of 9°. The hard component of the X-ray spectrum may be explained by the companion accreting mass from the wind of the Roche lobe filling primary, while the softer component may be due to colliding winds. A companion with a stronger wind than the primary could produce the latter and would be consistent with models of the observed diffuse X-ray emission detected in the nebula. The diffuse X-rays may also be powered by the jets of up to 180 km s−1, and active accretion would imply that they may be the first active jets of a post-common-envelope PN, potentially making NGC 2392 an invaluable laboratory to study jet formation physics. The 1.9 d orbital period rules out a double-degenerate merger leading to a Type Ia supernova, and the weak wind of the primary likely also precludes a single-degenerate scenario. We suggest that a hard X-ray spectrum, in the absence of a late-type companion, could be a powerful tool to identify accreting WD companions.

Introduction Greater resilience is associated with the ability to self-organise, and with social learning as part of a process of adaptation and transformation (Goldstein 341). This article deals with responses to a crisis in a Norwegian community in the late 1880s, and with some of the many internal conflicts it caused. The crisis and the subsequent conflicts in this particular community, Volda, were caused by a number of processes, driven mostly by external forces and closely linked to the expansion of the capitalist mode of production in rural Norway. But the crisis also reflects a growing nationalism in Norway. In the late 1880s, all these causes seemed to come together in Volda, a small community consisting mostly of independent small farmers and of fishers. The article employs the concept of ‘resilience’ and the theory of resilience in order better to understand how individuals and the community reacted to crisis and conflict in Volda in late 1880, experiences which will cast light on the history of the late 1880s in Volda, and on individuals and communities elsewhere which have also experienced such crises. Theoretical Perspectives Some understandings of social resilience inspired by systems theory and ecology focus on a society’s ability to maintain existing structures. Reducing conflict to promote greater collaboration and resilience, however, may become a reactionary strategy, perpetuating inequalities (Arthur, Friend and Marschke). Instead, the understanding of resilience could be enriched by drawing on ecological perspectives that see conflict as an integral aspect of a diverse ecology in continuous development. In the same vein, Grove has argued that some approaches to anticipatory politics fashion subjects to withstand ‘shocks and responding to adversity through modern institutions such as human rights and the social contract, rather than mobilising against the sources of insecurity’. As an alternative, radical politics of resilience ought to explore political alternatives to the existing order of things. Methodology According to Hall and Lamont, understanding “how individuals, communities, and societies secured their well-being” in the face of the challenges imposed by neoliberalism is a “problem of understanding the bases for social resilience”. This article takes a similarly broad approach to understanding resilience, focusing on a small group of people within a relatively small community to understand how they attempted to secure their well-being in the face of the challenges posed by capitalism and growing nationalism. The main interest, however, is not resilience understood as something that exists or is being produced within this small group, but, rather, how this group produced social imaginaries of the past and the future in cooperation and conflict with other groups in the same community. The research proceeds to analyse the contributions mainly of six members of this small group. It draws on existing literature on the history of the community in the late 1800s and, in particular, biographies of Synnøve Riste (Øyehaug) and Rasmus Steinsvik (Gausemel). In addition, the research builds on original empirical research of approximately 500 articles written by the members of the group in the period from 1887 to 1895 and published in the newspapers Vestmannen, Fedraheimen and 17de Mai; and will try to re-tell a history of key events, referring to a selection of these articles. A Story about Being a Woman in Volda in the Late 1880s This history begins with a letter from Synnøve Riste, a young peasant woman and daughter of a local member of parliament, to Anders Hovden, a friend and theology student. In the letter, Synnøve Riste told her friend about something she just had experienced and had found disturbing (more details in Øyehaug). She first sets her story in the context of an evangelical awakening that was gaining momentum in the community. There was one preacher in particular who seemed to have become very popular among the young women. He had few problems when it comes to women, she wrote, ironically. Curious about the whole thing, Synnøve decided to attend a meeting to see for herself what was going on. The preacher noticed her among the group of young women. He turned his attention towards her and scolded her for her apparent lack of religious fervour. In the letter she explained the feeling of shame that came over her when the preacher singled her out for public criticism. But the feeling of shame soon gave way to anger, she wrote, before adding that the worst part of it was ‘not being able to speak back’; as a woman at a religious meeting she had to hold her tongue. Synnøve Riste was worried about the consequences of the religious awakening. She asked her friend to do something. Could he perhaps write a poem for the weekly newspaper the group had begun to publish only a few months earlier? Anders Hovden duly complied. The poem was published, anonymously, on Wednesday 17 March 1888. Previously, the poem says, women enjoyed the freedom to roam the mountains and valleys. Now, however, a dark mood had come over the young women. ‘Use your mind! Let the madness end! Throw off the blood sucker! And let the world see that you are a woman!’ The puritans appreciated neither the poem nor the newspaper. The newspaper was published by the same group of young men and women who had already organised a private language school for those who wanted to learn to read and write New Norwegian, a ‘new’ language based on the old dialects stemming from the time before Norway lost its independence and became a part of Denmark and then, after 1814, Sweden. At the language school the students read and discussed translations of Karl Marx and the anarchist Peter Kropotkin. The newspaper quickly grew radical. It reported on the riots following the hanging of the Haymarket Anarchists in Chicago in 1886. It advocated women’s suffrage, agitated against capitalism, argued that peasants and small farmers must learn solidarity from the industrial workers defended a young woman in Oslo who was convicted of killing her newborn baby and published articles from international socialist and anarchist newspapers and magazines. Social Causes for Individual Resilience and Collaborative Resilience Recent literature on developmental psychology link resilience to ‘the availability of close attachments or a supportive and disciplined environment’ (Hall and Lamont 13). Some psychologists have studied how individuals feel empowered or constrained by their environment. Synnøve Riste clearly felt constrained by developments in her social world, but was also resourceful enough to find ways to resist and engage in transformational social action on many levels. According to contemporary testimonies, Synnøve Riste must have been an extraordinary woman (Steinsvik "Synnøve Riste"). She was born Synnøve Aarflot, but later married Per Riste and took his family name. The Aarflot family was relatively well-off and locally influential, although the farms were quite small by European standards. Both her father and her uncle served as members of parliament for the (‘left’) Liberal Party. From a young age she took responsibility for her younger siblings and for the family farm, as her father spent much time in the capital. Her grandfather had been granted the privilege of printing books and newspapers, which meant that she grew up with easy access to current news and debates. She married a man of her own choosing; a man substantially older than herself, but with a reputation for liberal ideas on language, education and social issues. Psychological approaches to resilience consider the influence of cognitive ability, self-perception and emotional regulation, in addition to social networks and community support, as important sources of resilience (Lamont, Welburn and Fleming). Synnøve Riste’s friend and lover, Rasmus Steinsvik, later described her as ‘a mainspring’ of social activity. She did not only rely on family, social networks and community support to resist stigmatisation from the puritans, but she was herself a driving force behind social activities that produced new knowledge and generated communities of support for others. Lamont, Welburn and Fleming underline the importance for social resilience of cultural repertoires and the availability of ‘alternative ways of understanding social reality’ (Lamont, Welburn and Fleming). Many of the social activities Synnøve Riste instigated served as arenas for debate and collaborative activity to develop alternative understandings of the social reality of the community. In 1887, Synnøve Riste had relied on support from her extended family to found the newspaper Vestmannen, but as the group around the language school and newspaper gradually produced more radical alternative understandings of the social reality they came increasingly into conflict with less radical members of the Liberal Party. Her uncle owned the printing press where Vestmannen was printed. He was also a member of parliament seeking re-election. And he was certainly not amused when Rasmus Steinsvik, editor of Vestmannen, published an article reprimanding him for his lacklustre performance in general and his unprincipled voting in support of a budget allocating the Swedish king a substantial amount of money. Steinsvik advised the readers to vote instead for Per Riste, Synnøve Riste’s liberal husband and director of the language school. The uncle stopped printing the newspaper. Social Resilience in Volda The growing social conflicts in Volda might be taken to indicate a lack of resilience. This, however, would be a mistake. Social connectedness is an important source of social resilience (Barnes and Hall 226). Strong ties to family and friends matter, as does membership in associations. Dense networks of social connectedness are related to well-being and social resilience. Inversely, high levels of inequality seem to be linked to low levels of resilience. Participation in democratic processes has also been found to be an important source of resilience (Barnes and Hall 229). Volda was a small community with relatively low levels of inequality and local cultural traditions underlining the importance of cooperation and the obligations of everyone to participate in various forms of communal work. Similarly, even though a couple of families dominated local politics, there was no significant socioeconomic division between the average and the more prosperous farmers. Traditionally, women on the small, independent farms participated actively in most aspects of social life. Volda would thus score high on most indicators predicting social resilience. Reading the local newspapers confirms this impression of high levels of social resilience. In fact, this small community of only a few hundred families produced two competing newspapers at the time. Vestmannen dedicated ample space to issues related to education and schools, including adult education, reflecting the fact that Volda was emerging as a local educational centre; local youths attending schools outside the community regularly wrote articles in the newspaper to share the new knowledge they had attained with other members of the community. The topics were in large part related to farming, earth sciences, meteorology and fisheries. Vestmannen also reported on other local associations and activities. The local newspapers reported on numerous political meetings and public debates. The Liberal Party was traditionally the strongest political party in Volda and pushed for greater independence from Sweden, but was divided between moderates and radicals. The radicals joined workers and socialists in demanding universal suffrage, including, as we have seen, women’s right to vote. The left libertarians in Volda organised a ‘radical left’ faction of the Liberal Party and in the run-up to the elections in 1888 numerous rallies were arranged. In some parts of the municipality the youth set up independent and often quite radical youth organisations, while others established a ‘book discussion’. The language issue developed into a particularly powerful source for social resilience. All members of the community shared the experience of having to write and speak a foreign language when communicating with authorities or during higher education. It was a shared experience of discrimination that contributed to producing a common identity. Hing has shown that those who value their in-group ‘can draw on this positive identity to provide a sense of self-worth that offers resilience’. The struggle for recognition stimulated locals to arrange independent activities, and it was in fact through the burgeoning movement for a New Norwegian language that the local radicals in Volda first encountered radical literature that helped them reframe the problems and issues of their social world. In his biography of Ivar Mortensson Egnund, editor of the newspaper Fedraheimen and a lifelong collaborator of Rasmus Steinsvik, Klaus Langen has argued that Mortensson Egnund saw the ideal type of community imagined by the anarchist Leo Tolstoy in the small Norwegian communities of independent small farmers, a potential model for cooperation, participation and freedom. It was not an uncritical perspective, however. The left libertarians were constantly involved in clashes with what they saw as repressive forces within the communities. It is probably more correct to say that they believed that the potential existed, within these communities, for freedom to flourish. Most importantly, however, reading Fedraheimen, and particularly the journalist, editor and novelist Arne Garborg, infused this group of local radicals with anti-capitalist perspectives to be used to make sense of the processes of change that affected the community. One of Garborg’s biographers, claims that no Norwegian has ever been more fundamentally anti-capitalist than Garborg (Thesen). This anti-capitalism helped the radicals in Volda to understand the local conflicts and the evangelical awakening as symptoms of a deeper and more fundamental development driven by capitalism. A series of article in Vestmannen called for solidarity and unity between small farmers and the growing urban class of industrial workers. Science and Modernity The left libertarians put their hope in science and modernity to improve the lives of people. They believed that education was the key to move forward and get rid of the old and bad ways of doing things. The newspaper was reporting the latest advances in natural sciences and life sciences. It reported enthusiastically about the marvels of electricity, and speculated about a future in which Norway could exploit the waterfalls to generate it on a large scale. Vestmannen printed articles in defence of Darwinism (Egnund), new insights from astronomy (Steinsvik "Kva Den Nye Astronomien"), health sciences, agronomy, new methods of fishing and farming – and much more. This was a time when such matters mattered. Reports on new advances in meteorology in the newspaper appeared next to harrowing reports about the devastating effects of a storm that surprised local fishermen at sea where many men regularly paid with their lives. Hunger was still a constant threat in the harsh winter months, so new knowledge that could improve the harvest was most welcome. Leprosy and other diseases continued to be serious problems in this region of Norway. Health could not be taken lightly, and the left libertarians believed that science and knowledge was the only way forward. ‘Knowledge is a sweet fruit,’ Vestmannen wrote. Reporting on Darwinism and astronomy again pitted Vestmannen against the puritans. On several occasions the newspaper reported on confrontations between those who promoted science and those who defended a fundamentalist view of the Bible. In November 1888 the signature ‘-t’ published an article on a meeting that had taken place a few days earlier in a small village not far from Volda (Unknown). The article described how local teachers and other participants were scolded for holding liberal views on science and religion. Anyone who expressed the view that the Bible should not be interpreted literally risked being stigmatised and ostracised. It is tempting to label the group of left libertarians ‘positivists’ or ‘modernists’, but that would be unfair. Arne Garborg, the group’s most important source of inspiration, was indeed inspired by Émile Zola and the French naturalists. Garborg had argued that nothing less than the uncompromising search for truth was acceptable. Nevertheless, he did not believe in objectivity; Garborg and his followers agreed that it was not possible or even desirable to be anything else than subjective. Adaptation or Transformation? PM Giærder, a friend of Rasmus Steinsvik’s, built a new printing press with the help of local blacksmiths, so the newspaper could keep afloat for a few more months. Finally, however, in 1888, the editor and the printer took the printing press with them and moved to Tynset, another small community to the east. There they joined forces with another dwindling left libertarian publication, Fedraheimen. Generations later, more details emerged about the hurried exit from Volda. Synnøve Riste had become pregnant, but not by her husband Per. She was pregnant by Rasmus Steinsvik, the editor of Vestmannen and co-founder of the language school. And then, after giving birth to a baby daughter she fell ill and died. The former friends Per and Rasmus were now enemies and the group of left libertarians in Volda fell apart. It would be too easy to conclude that the left libertarians failed to transform the community and a closer look would reveal a more nuanced picture. Key members of the radical group went on to play important roles on the local and national political scene. Locally, the remaining members of the group formed new alliances with former opponents to continue the language struggle. The local church gradually began to sympathise with those who agitated for a new language based on the Norwegian dialects. The radical faction of the Liberal Party grew in importance as the conflict with Sweden over the hated union intensified. The anarchists Garborg and Steinsvik became successful editors of a radical national newspaper, 17de Mai, while two other members of the small group of radicals went on to become mayors of Volda. One was later elected member of parliament for the Liberal Party. Many of the more radical anarchist and communist ideas failed to make an impact on society. However, on issues such as women’s rights, voting and science, the left libertarians left a lasting impression on the community. It is fair to say that they contributed to transforming their society in many and lasting ways. Conclusion This study of crisis and conflict in Volda indicate that conflict can play an important role in social learning and collective creativity in resilient communities. There is a tendency, in parts of resilience literature, to view resilient communities as harmonious wholes without rifts or clashes of interests (see for instance Goldstein; Arthur, Friend and Marschke). Instead, conflicts should rather be understood as a natural aspect of any society adapting and transforming itself to respond to crisis. Future research on social resilience could benefit from an ecological understanding of nature that accepts polarisation and conflict as a natural part of ecology and which helps us to reach deeper understandings of the social world, also fostering learning, creativity and the production of alternative political solutions. This research has indicated the importance of social imaginaries of the past. Collective memories of ‘what everybody knows that everybody else knows’ about ‘what has worked in the past’ form the basis for producing ideas about how to create collective action (Swidler 338, 39). Historical institutions are pivotal in producing schemas which are default options for collective action. In Volda, the left libertarians imagined a potential for freedom in the past of the community; this formed the basis for producing an alternative social imaginary of the future of the community. The social imaginary was not, however, based only on local experience and collective memory of the past. Theories played an important role in the process of trying to understand the past and the present in order to imagine future alternatives. The conflicts themselves stimulated the radicals to search more widely and probe more deeply for alternative explanations to the problems they experienced. This search led them to new insights which were sometimes adopted by the local community and, in some cases, helped to transform social life in the long-run. References Arthur, Robert, Richard Friend, and Melissa Marschke. "Fostering Collaborative Resilience through Adaptive Comanagement: Reconciling Theory and Practice in the Management of Fisheries in the Mekong Region." 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Fürstbischof Julius Echter von Mespelbrunn (1573 – 1617) und die Hexenverfolgungen im Hochstift Würzburg (Hexenforschung, 16), Bielefeld 2017, Verlag für Regionalgeschichte, 252 S. / Abb., € 24,00. (Rainer Walz, Bochum) Sidler, Daniel, Heiligkeit aushandeln. Katholische Reform und lokale Glaubenspraxis in der Eidgenossenschaft (1560 – 1790) (Campus Historische Studien, 75), Frankfurt a. M. / New York 2017, Campus, 593 S. / Abb., € 58,00. (Heinrich Richard Schmidt, Bern) Moring, Beatrice / Richard Wall, Widows in European Economy and Society, 1600 – 1920, Woodbridge / Rochester 2017, The Boydell Press, XIII u. 327 S. / Abb., £ 75,00. (Margareth Lanzinger, Wien) Katsiardi-Hering, Olga / Maria A. Stassinopoulou (Hrsg.), Across the Danube. Southeastern Europeans and Their Travelling Identities (17th–19th C.) (Studies in Global Social History, 27; Studies in Global Migration History, 9), Leiden / Boston 2017, Brill, VIII u. 330 S. / Abb., € 110,00. (Olivia Spiridon, Tübingen) „wobei mich der liebe Gott wunderlich beschutzet“. Die Schreibkalender des Clamor Eberhard von dem Bussche zu Hünnefeld (1611 – 1666). Edition mit Kommentar, hrsg. v. Lene Freifrau von dem Bussche-Hünnefeld / Stephanie Haberer, [Bramsche] 2017, Rasch, 216 S. / Abb., € 34,50. (Helga Meise, Reims) Rohrschneider, Michael / Anuschka Tischer (Hrsg.), Dynamik durch Gewalt? Der Dreißigjährige Krieg (1618 – 1648) als Faktor der Wandlungsprozesse des 17. Jahrhunderts (Schriftenreihe zur Neueren Geschichte, 38; Neue Folge, 1), Münster 2018, Aschendorff, VII u. 342 S. / Abb., € 48,00. (Claire Gantet, Fribourg) Schloms, Antje, Institutionelle Waisenfürsorge im Alten Reich 1648 – 1806. Statistische Analyse und Fallbeispiele (Beiträge zur Wirtschafts- und Sozialgeschichte, 129), Stuttgart 2017, Steiner, 395 S., € 62,00. (Iris Ritzmann, Zürich) Mühling, Christian, Die europäische Debatte über den Religionskrieg (1679 – 1714). Konfessionelle Memoria und internationale Politik im Zeitalter Ludwigs XIV. (Veröffentlichungen des Instituts für europäische Geschichte Mainz, 250), Göttingen 2018, Vandenhoeck &amp; Ruprecht, 587 S., € 85,00. (Cornel Zwierlein, Bamberg) Dietz, Bettina, Das System der Natur. Die kollaborative Wissenskultur der Botanik im 18. Jahrhundert, Köln / Weimar / Wien 2017, Böhlau, 216 S., € 35,00. (Flemming Schock, Leipzig) Friedrich, Markus / Alexander Schunka (Hrsg.), Reporting Christian Missions in the Eighteenth Century. Communication, Culture of Knowledge and Regular Publication in a Cross-Confessional Perspective (Jabloniana, 8), Wiesbaden 2017, Harrassowitz, 196 S., € 52,00. (Nadine Amsler, Frankfurt a. M.) Berkovich, Ilya, Motivation in War. The Experience of Common Soldiers in Old-Regime Europe, Cambridge / New York 2017, Cambridge University Press, XII u. 280 S. / graph. Darst., £ 22,99. (Marian Füssel, Göttingen) Stöckl, Alexandra, Der Principalkommissar. Formen und Bedeutung sozio-politischer Repräsentation im Hause Thurn und Taxis (Thurn und Taxis Studien. Neue Folge, 10), Regensburg 2018, Pustet, VII u. 280 S., € 34,95. (Dorothée Goetze, Bonn) Wunder, Dieter, Der Adel im Hessen des 18. Jahrhunderts – Herrenstand und Fürstendienst. Grundlagen einer Sozialgeschichte des Adels in Hessen (Veröffentlichungen der Historischen Kommission für Hessen, 84), Marburg 2016, Historische Kommission für Hessen, XIV u. 844 S. / Abb., € 39,00. (Alexander Kästner, Dresden) Mährle, Wolfgang (Hrsg.), Aufgeklärte Herrschaft im Konflikt. Herzog Carl Eugen von Württemberg 1728 – 1793. Tagung des Arbeitskreises für Landes- und Ortsgeschichte im Verband der württembergischen Geschichts- und Altertumsvereine am 4. und 5. Dezember 2014 im Hauptstaatsarchiv Stuttgart (Geschichte Württembergs, 1), Stuttgart 2017, Kohlhammer, 354 S. / Abb., € 25,00. (Dietmar Schiersner, Weingarten) Bennett, Rachel E., Capital Punishment and the Criminal Corpse in Scotland, 1740 – 1834 (Palgrave Historical Studies in the Criminal Corpse and its Afterlife), Cham 2018, Palgrave Macmillan, XV u. 237 S., € 29,96. (Benjamin Seebröker, Dresden) York, Neil L., The American Revolution, 1760 – 1790. New Nation as New Empire, New York / London 2016, Routledge, XIII u. 151 S. / Karten, Hardcover, £ 125,00. (Volker Depkat, Regensburg) Richter, Roland, Amerikanische Revolution und niederländische Finanzanleihen 1776 – 1782. Die Rolle John Adams’ und der Amsterdamer Finanzhäuser bei der diplomatischen Anerkennung der USA (Niederlande-Studien, 57), Münster / New York 2016, Waxmann, 185 S. / Abb., € 29,90. (Volker Depkat, Regensburg) Steiner, Philip, Die Landstände in Steiermark, Kärnten und Krain und die josephinischen Reformen. Bedrohungskommunikation angesichts konkurrierender Ordnungsvorstellungen (1789 – 1792), Münster 2017, Aschendorff, 608 S. / Abb., € 59,00 (Simon Karstens, Trier)

Populist communication, in this entry, refers to the occurrence of a) specific messages that are seen as the expression of populist ideology and b) characteristic style elements that are often associated with these messages expressing populist ideology in political actors’ (or other actors such as journalists’ or citizens’) communication (Ernst et al., 2019; De Vreese et al., 2018). Field of application/theoretical foundation: Populism has been defined in various terms; e.g., as Ideology (Canovan, 1999; Mudde, 2004), set of ideas (Hawkins et al., 2018, Taggart, 2000), discourse (Laclau, 2005; Mouffe, 2018), political style (Moffit, 2016), communication style (Jagers & Walgrave, 2007), or political strategy (Weyland, 2017). Thus, there have been numerous operationalizations of populism or populist communication in content analyses that cannot all be accounted for here. This entry specifically follows a communication-centered perspective (Stanyer et al., 2016; De Vreese et al., 2018). Jagers & Walgrave (2007), in a pioneer study on populist communication, define populism as a political communication style “essentially displaying proximity of the people, while at the same time taking an anti-establishment stance and stressing the (ideal) homogeneity of the people by excluding specific population segments.” In a more recent study, Ernst et al. (2019) differentiate between populist communication content and populist communication style. Populist communication content refers to the communicative representation of the populist ideology (what is being said) that can be expressed in the form of populist key messages. Depending on the parsimony of the definition, populist ideology comprises three or four dimensions: people-centrism, anti-elitism, restoring sovereignty, and exclusion (e.g., De Vreese et al., 2018; Mudde, 2004; Jagers & Walgrave, 2007; Wirth et al., 2016). In distinction to the content, Ernst et al. (2019) define populist communication style as the use of populism-related style elements (how something is said) (see also De Vreese et al., 2018; Bracciale & Martella, 2018). Communication-centered content analyses of populist communication are often carried out in three steps. First, specific characteristics of populist communication (e.g., populist key messages or stylistic elements) are identified. Second, the occurrence of these individual elements is then coded either on the statement level (e.g. Ernst et al., 2019; Wirth et al., 2016), excerpts level (Jagers & Walgrave, 2007), or on the text/article level (e.g. Blassnig et al, 2019). Third, the level of populism is determined using different indices for populist communication as a whole (e.g. maximum indices; Blassnig et al., 2019; Ernst et al., 2019) or for the individual dimensions separately (e.g., Jagers & Walgrave, 2007). Populism indices can be calculated at the statement level, text level, or actor level. References/combination with other methods of data collection: Whereas this entry focuses on quantitative and deductive approaches, populist communication has also been investigated using qualitative or inductive approaches (e.g., Wodak, 2015), especially in studies following a more actor-centered approach (Stanyer et al., 2016). Most studies on populist communication have used manual content analysis. Yet, some analyses have also applied automated approaches to investigate the occurrence of populist communication in texts (e.g., Hawkins & Castanho Silva, 2018). Example studies: Blassnig et al., (2019); Bracialle & Martella (2017); Ernst et al., (2019); Jagers & Walgrave, (2007) Table 2: Summary of a selection of studies on populist communication Author(s) Sample Unit of Analysis Values Reliability Jagers & Walgrave, 2007 Content type: political party broadcasts (PPB) Country: Belgium (Flemish part) Political actors: six Belgian-Flemish parties Outlets: 20 PPBs per party Sampling period: 1999 - 2001 Sample size: 1,200 PPB excerpts Unit of analysis: excerpts including ‘thin’ populism (references to the people) Level of analysis: excerpt level and actor level People-index: multiplication of the proportion and intensity of references to the people for each party Anti-state-index: number of anti-state excerpts * average intension anti-state excerpts (1-5) per party Anti-politics-index: number of anti-politics excerpts * average intension anti-politics excerpts (1-5) per party Anti-media-index: number of anti-media excerpts * average intension anti-media excerpts (1-5) per party Anti-establishment-index: anti-state + anti-politics + anti-media per party Exclusivity-index: J-scores; (positive – negative evaluations) / (positive + neutral + negative evaluations of specific population categories) References to the people: terms referring to the population (as a whole or population categories), that cover the people “in political terms”, meaning the “political entity” Anti-state: failure of the state with regard to (1) single failure, (2) systematic failure, (3) public service should be abolished, (4) all public services are criticized at once, (5) the system Anti-politics: criticism directed towards (1) policy measure or present situation, (2) policy, (3) politician, (4) party, (5) group of parties, (6) all parties. (7) the system Anti-media: media targets of criticism; (1) newspaper/ magazine/ tv channel, (2) group of media, (3) all (the) media Evaluation of specific population categories: positive, neutral, negative (for further restrictions for the individual variables and more detailed instructions see the methodological appendix by Jagers & Walgrave, 2007) Reliability is not reported Ernst, Blassnig, Engesser, Büchel, & Esser (2019) (See also Ernst et al., 2018; Ernst, Esser et al., 2019; Wirth et al., 2016) Content type: statements by politicians expressing either a political position, an elaboration on a political issue, or an evaluation/ attribution of a target actor Countries: CH, DE, IT, FR, UK, US Political actors: 98 politicians from 31 parties Outlets: political talk shows (2 per country), politicians’ Facebook and Twitter accounts Sampling period: April through May 2015 Sample size: n = 2’067 (n = 969 talk show statements, n = 734 Facebook posts, and n = 364 Tweets Unit of analysis: a single statement by a politician on a target actor or an issue Level of analysis: statement level and actor level Populism index: Maximum index based on the nine populist key messages and seven stylistic elements (0/1) Populist key messages: Anti-elitism: discrediting the elite, blaming the elite, detaching the elite from the people People-centrism: stressing the people’s virtues, praising the people’s achievements, stating a monolithic people, demonstrating closeness to the people Restoring sovereignty: demanding popular sovereignty, denying elite sovereignty Populist style elements: Negativity: negativism, crisis rhetoric Emotionality: emotional tone, absolutism, patriotism) Sociability: colloquialism, intimization (all items were coded as dummy variables based on more detailed sub-categories) Brennan & Prediger’s kappa average = 0.91 (³0.65) Blassnig, Ernst, Büchel, Engesser, & Esser (2019) Content type: election news coverage about immigration and adjacent reader comments Countries: CH, FR, UK Actors/Speakers: politicians, journalists, and citizens Outlets: 6 online news outlets per country Sampling period: six weeks before the respective election days. CH: September to October 2015; FR: April to May 2017; UK: April to May 2015 Sample size: n = 493 news articles and n = 2904 reader comments Unit of analysis: news article / reader comment Level of analysis: article level Populism index: Maximum index based on the twelve populist key messages (0/1) Populist key messages: Anti-elitism: discrediting the elite, blaming the elite, detaching the elite from the people People-centrism: praising the people’s virtues, praising the people’s achievements, describing the people as homogenous, demonstrating closeness to the people Restoring sovereignty: demanding popular sovereignty, denying elite sovereignty Exclusion: discrediting specific social groups, blaming specific social groups, excluding specific social groups from the people (all items were coded as dummy variables) Brennan & Prediger’s kappa average = 0.75 Bracciale & Martella (2017) Content type: politicians’ tweets Country: Italy Political actors: 5 party leaders Outlets: leaders’ Twiter timelines Sampling period: 1 January 2015 to 1 July 2016 Sample size: n = 7,772 Unit of analysis: tweets Level of analysis: tweets, actors Indices: Populist ideology: three additive synthetic dichotomous indices adding together the indicators for each of the three dimensions of populism (sovereignty of the people, attacking the elite, ostracizing others) The variables for political communication style were summarized using multiple correspondence analysis (MCA) into two dimensions: communicative mode (positive vs. negative) and communicative focus (personalization vs. political/ campaign) Political communication style: Stagecraft: emotionalisation; informality, instrumental actualization, intimisation, negative affect, simplification, storytelling, taboo breaker, vulgarism Register (communicative tone): referential/ neutral, aggressive/ provocative, humorous/ ironic, conversational/ participatory Topic: political issues, policy issues, campaign issues, personal issues, current affairs Function: campaign updating, self-promotion, setting the agenda, position-taking, call to action, opposition/ violence, endorsement, irony, request for interaction, pointless babble Populist ideology: Emphasizing sovereignty of the people: refers to the people, refers to ‘ad hoc’ people, direct representation Attacking the elite: generic anti-establishment, political anti-establishment, economic anti-establishment, EU anti-establishment, institutional anti-establishment, anti-elitism media, anti-elitism intellectuals Ostracizing others: dangerous others, authoritarianism (all individual indicators were coded as dummy variables) Krippendorff's Alpha > .83 References Blassnig, S., Ernst, N., Büchel, F., Engesser, S., & Esser, F. (2019). Populism in online election coverage. Journalism Studies, 20(8), 1110–1129. https://doi.org/10.1080/1461670X.2018.1487802 Bracciale, R., & Martella, A. (2017). Define the populist political communication style: the case of Italian political leaders on Twitter. Information, Communication & Society, 20(9), 1310–1329. https://doi.org/10.1080/1369118X.2017.1328522 Canovan, M. (1999). Trust the people! Populism and the two faces of democracy. Political Studies, 47(1), 2–16. https://doi.org/10.1111/1467-9248.00184 Cranmer, M. (2011). Populist communication and publicity: An empirical study of contextual differences in Switzerland. Swiss Political Science Review, 17(3), 286–307. https://doi.org/10.1111/j.1662-6370.2011.02019.x De Vreese, C. H., Esser, F., Aalberg, T., Reinemann, C., & Stanyer, J. (2018). Populism as an expression of political communication content and style: A new perspective. The International Journal of Press/Politics, 23(4), 423-438. https://doi.org/10.1177/1940161218790035 Engesser, S., Fawzi, N., & Larsson, A. O. (2017). Populist online communication: Introduction to the special issue. Information, Communication & Society, 20(9), 1279–1292. https://doi.org/10.1080/1369118X.2017.1328525 Ernst, N., Blassnig, S., Engesser, S., Büchel, F., & Esser, F. (2019). Populists prefer social media over talk shows: An analysis of populist messages and stylistic elements across six countries. Social Media + Society, 5(1), 1-14. https://doi.org/10.1177/2056305118823358 Hawkins, K. A., Carlin, R. E., Littvay, L., & Rovira Kaltwasser, C. (Eds.). (2018). Extremism and democracy. The ideational approach to populism: Concept, theory, and analysis. Routledge. Haswkins, K. A., & Castanho Silva, B. (2018). Textual analysis: big data approaches. In K. A. Hawkins, R. E. Carlin, L. Littvay, & C. Rovira Kaltwasser (Eds.). Extremism and democracy. The ideational approach to populism: Concept, theory, and analysis (pp. 27-48). Routledge. Jagers, J., & Walgrave, S. (2007). Populism as political communication style: An empirical study of political parties' discourse in Belgium. European Journal of Political Research, 46(3), 319–345. https://doi.org/10.1111/j.1475-6765.2006.00690.x Laclau, E. (2005). On populist reason. London: Verso. Moffitt, B. (2016). The global rise of populism: Performance, political style, and representation. Stanford University Press. Mudde, C. (2004). The populist Zeitgeist. Government and Opposition, 39(4), 542–563. https://doi.org/10.1111/j.1477-7053.2004.00135.x Stanyer, J., Salgado, S., & Strömbäck, J. (2017). Populist actors as communicators or political actors as populist communicators: Cross-national findings and perspectives. In T. Aalberg, F. Esser, C. Reinemann, J. Strömbäck, & C. H. de Vreese (Eds.), Populist political communication in Europe (pp. 353–364). Routledge. Taggart, P. (2000). Populism. Concepts in the social sciences. Open University Press. Wirth, W., Esser, F., Engesser, S., Wirz, D. S., Schulz, A., Ernst, N., . . . Schemer, C. (2016). The appeal of populist ideas, strategies and styles: A theoretical model and research design for analyzing populist political communication. Zurich: NCCR Democracy, Working Paper No. 88, pp. 1–60. https://doi.org/10.5167/uzh-127461 Wodak, R. (2015). The Politics of Fear: What Right-Wing Populist Discourses Mean. SAGE Publications.

Abstract STUDY QUESTION What is the role of the Hippo and PI3K/Akt pathway in follicles during ovarian tissue culture in tissue derived from oncological patients and transgender men? SUMMARY ANSWER Results highlight a Hippo pathway driven primordial follicle activation in vitro, predominantly from Day 0 to Day 4. WHAT IS KNOWN ALREADY In-vitro ovarian tissue culture aims at activating and maturing primordial follicles for fertility restoration in patients with a threatened ovarian reserve. Not all patients are eligible for ovarian cortex transplantation and therefore several groups are attempting to culture ovarian tissue in-vitro. Cortex fragmentation disrupts the Hippo pathway, leading to increased expression of downstream growth factors and follicle growth. The PI3K/Akt pathway is considered the intracellular pathway to where different extracellular factors involved in primordial follicle activation in-vivo converge. In order to optimise current ovarian tissue culture models, information on progression of these pathways during tissue culture is mandatory. STUDY DESIGN, SIZE, DURATION The first step of a multistep cortex culture system was performed using 144 ovarian cortex pieces from a total of six patients. Per patient, 24 cortical strips were cultured for 6 days and six pieces per patient were collected for downstream analysis of follicle development and Hippo and PI3K/Akt pathway targets every second day. PARTICIPANTS/MATERIALS, SETTING, METHODS Ovarian tissue was obtained from oncological (N = 3; 28.67 ± 4.51 years) and transgender (N = 3; 23.33 ± 1.53 years) patients. Follicles were analysed using haematoxylin-eosin staining and pathways were studied using immunohistochemistry and precise follicle excision by laser capture micro-dissection for RT-qPCR analysis. MIQE guidelines for RT-qPCR were pursued. Reference gene selection (GAPDH, RPL3A, 18s rRNA) was performed using GeNorm Reference Gene Selection Kit. Statistical analysis was conducted with IBM SPSS Statistics 23 (Poisson regression, negative binomial regression, ANOVA and paired t-test). MAIN RESULTS AND THE ROLE OF CHANCE Immunohistochemical analysis confirmed a Hippo pathway driven primordial follicle activation due to mechanical manipulation of the cortical strips. Ovarian tissue preparation and culture induced the inhibitory phosphorylated Yes-associated protein (pYAP) to disappear in granulosa cells of primordial follicles on Day 2. The stimulatory YAP on the contrary appeared in primordial granulosa cells over increasing culture days. Looking at the YAP target connective tissue growth factor (CTGF), a significantly up-regulated CTGF was noted in primordial follicles when comparing Day 2 and Day 4 (ratio Day 2/4 = 0.082; P &lt; 0.05), clearly showing an effect on the Hippo pathway in primordial follicles during tissue culture. Follicle classification showed a significant drop in estimated primordial follicle counts in the oncological cohort (−78%; P = 0.021) on Day 2 and in the transgender cohort on Day 4 (−634%; P = 0.008). Intermediate follicle counts showed a non-significant increasing trend to during culture and this follicle recruitment and growth resulted in a significant rise in estimated primary follicle counts on Day 6 in oncological patients (170%; P = 0.025) and, although limited in absolute numbers, a significant increase in secondary follicles on Day 4 (367%; P = 0.021) in the transgender cohort. Subsequent antral follicle development could not be observed. LIMITATIONS, REASONS FOR CAUTION A limitation is the small sample size, inherent to this study subject, especially as a large amount of tissue was needed per patient to reduce inter-patient variation in different downstream analysis techniques. A particular and specific weakness of this study is the inability to include an age-matched control group. WIDER IMPLICATIONS OF THE FINDINGS These findings support an adapted tissue preparation for Hippo pathway disruption and a shorter first phase of tissue culture. This work may also have implications for transplantation of cryopreserved tissue as larger strips (and thus slower burnout due to less Hippo pathway disruption) could be a benefit. STUDY FUNDING/COMPETING INTEREST(S) This research was financially supported by the Foundation Against Cancer (Stichting tegen Kanker, TBMT001816N), the Flemish Foundation of Scientific Research (FWO Vlaanderen, FWO G0.065.11N10) and the Gender Identity Research and Education Society (GIRES) foundation. The authors declare no competing interests. TRIAL REGISTRATION NUMBER N/A.

Morning report (MR) is a valued educational experience in internal medicine training programs. Many senior residents and faculty have not received formal training in how to effectively facilitate MR. Faculty at the University of Toronto were surveyed to provide insights into what they felt were key elements for the successful facilitation of MR. These insights fell within 5 major categories: planning and preparation, the case, running the show, wrapping up and closing the loop.Résumé Le rapport du matin (RM) est un outil pédagogique précieux dans les programmes de formation en médecine interne. Nombre de résidents séniors et de membres du corps enseignant n’ont toutefois jamais reçu de formation officielle sur la façon de faciliter l’élaboration du RM. Nous avons sondé les membres du corps enseignant de l’université de Toronto pour avoir un aperçu de ce qu’ils percevaient comme étant des éléments-clés susceptibles d’améliorer grandement l’élaboration du RM. Les réponses reçues se répartissent en cinq principales catégories: la planification et la préparation du RM, les caractéristiques du cas évalué, l’importance et la façon de prendre en main le processus, le résumé des informations et l’art de « boucler la boucle». Morning report (MR) has long been an integral and valued part of Internal Medicine training programs in North America.1,2 Some residents recognize MR as the most important educational activity during their training.3 Medical students, residents and faculty typically attend MR. Although the structure and function of MR can vary across institutions, it usually involves a case-based discussion facilitated by a faculty member, chief medical resident (CMR), or other senior resident. The facilitator discusses pertinent aspects of one or more clinical cases to teach medical knowledge, clinical reasoning and other important aspects of physician competencies, such as communication and collaboration skills. 4 Residents have expressed a preference for an interactive teaching session led by an individual with extensive medical knowledge and excellent clinical acumen.5Despite trainees’ perceptions about the core educational function of MR and their preference for skilled facilitators, most residents and many faculty have never received any formal training on how to conduct an effective MR. This, coupled with a lack of resources in the literature, may contribute to feelings of trepidation about assuming the role of facilitator.6 Based on this need, we were invited by the organizing group of residents at the 2015 Canadian CMR Conference, held in Toronto, Canada, to lead a seminar to introduce CMRs to the principles of effective MR facilitation. The conference was attended by over 70 current and future CMRs. In preparation for this seminar, we reviewed available literature and found that practical guidelines on how to facilitate a successful MR were generally lacking. To help us to provide guidance and to capture broad opinions and experiences, we recruited a sample of 24 faculty at the University of Toronto, including many award-winning teachers whose experience in leading MR ranges from 3 to over 30 years. We asked them to provide insights into what they felt were key elements of facilitating a successful MR. While not a systematic collection of data, their insights taken together represent a broad experience base. Given the relative lack of evidence-based literature describing how to facilitate MR, we decided to disseminate a refined summary of the shared wisdom we uncovered in hopes that it would benefit other CMRs and junior faculty as they take on this challenging role.The insights provided fall within 5 main themes (Table 1) which are discussed below, followed by a brief discussion about future directions for MR:1) Planning and preparation2) The case3) Running the show4) Wrapping up5) Closing the LoopTable 1. Experience-Based Tips to Running an Effective Morning ReportPLANNING AND PREPARATION:1) Ensure audiovisual aids are present and working before starting. 2) Start and end on time. 3) Encourage all faculty to attend and participate. 4) Know the audience (including names).THE CASE:5) The case can be undifferentiated or one for which the diagnosis and even response to treatment is known. 6) There are pros and cons to the facilitator knowing details of the case in advance. 7) If details of the case are not known to the facilitator, determine with the person presenting if the discussion should be focused on diagnosis, management or other pertinent issues. 8) Cases need not be limited to inpatients and can include ambulatory cases and case simulations.RUNNING THE SHOW:9) Establish a respectful learning climate. 10) Personal anecdotes and reflections on past cases can engage the audience. 11) Ensure time is spent discuss learning issues valuable to all present. 12) Facilitate and engage in discussion rather than deliver a lecture. 13) Use a mix of pattern recognition (heuristics) and analytical reasoning strategies. 14) Start with a question that has an obvious answer if dealing with a quiet audience. 15) Promote volunteerism for answers as much as possible, but direct a question to a specific person if no one volunteers. 16) Begin by engaging the most junior learners and advance to involve senior learners. 17) Encourage resource stewardship and evidence-based medicine. 18) Acknowledge areas of uncertainty and don’t be afraid to say “I don’t know”. 19) Teaching “scripts” or the use of a systematic approach to developing a differential diagnosis can be used when discussing less familiar topics. 20) Highlight the variability in clinical approach amongst "the experts" in the room.WRAPPING-UP:21) Ensure there is time to summarize “take home points”. 22) Provide learners with the opportunity to summarize what they have learned.CLOSING THE LOOP23) Reinforcement of learning can include a distribution of a relevant paper or providing a summary of learning points via email or blog. 24) Maintain a case log to ensure a balanced curriculum. 25) Provide feedback to the case presenter and facilitator.Planning and Preparation It is important for the organizer and facilitator (these may or may not be the same person) to be diligent when preparing for MR. The person in charge of organizing MR should ensure that all necessary audiovisual equipment is in working order, which may be as simple as ensuring there is a whiteboard and working marker. To optimize housestaff attendance, the sessions and facilitators should be scheduled in a regular and predictable way. The lure of a light breakfast should not be underestimated and may add to the social aspect of this event. Sessions should begin and finish on time (or even slightly early). Ideally, deferring pages for all but critical clinical issues should occur. Having faculty regularly attend MR as audience participants, and not just as facilitators, improves the attendance of learners who see through role-modelling the importance of continuing medical education and lifelong learning. Faculty presence also raises the level of discussion around grey areas of diagnosis and management, providing trainees with a spectrum of opinions and approaches to clinical medicine, specifically role-modelling how faculty approach clinical uncertainty. The organizer must also ensure that someone, usually a trainee, is responsible for bringing the details of one or more clinical cases to be discussed.The facilitator should ensure they know the names and year of training of the housestaff in attendance. It is helpful if the organizer can provide a list (ideally with pictures) of those who will be in attendance for the facilitator to reference. Over time, this helps to develop a sense of community within the group. It also allows the facilitator to engage all participants and with the goals of first posing level-specific questions to the more junior learners and ending with the most senior learners.The Case The selected clinical case can be either a new patient seen in consultation in the past 24 hours or a patient that has been in hospital for some time and for whom results of investigations and response to treatment are known. Ideally, the majority of the cases selected should not involve particularly rare medical issues and should mirror the clinical case mix of patients being cared for by the trainees. Trainees will benefit more from discussions about common clinical problems rather. However, to highlight issues of diagnostic reasoning, it can be beneficial to occasionally discussing uncommon case including typical presentations of rare diseases or unusual presentations of common problems.The faculty surveyed expressed differing opinions when asked if they thought the details of the case should be known to the facilitator in advance. Knowing the details of the case in advance can ensure the facilitator is comfortable with the content area and allows them to focus on aspects of the case that they think will have the highest learning impact for trainees. However, when the case is not known to the facilitator, the audience will be more likely to garner insight into the clinical reasoning process of the facilitator. The opportunity to learn about the cognitive process that an “expert” uses when generating a differential diagnosis and formulating plans for investigation and management is potentially much more valuable than the discussion of content that could be read in a textbook or electronically. When the details of a case are not known, the discussion is more spontaneous and the lines of discussion are more reflective of the thoughts of the trainees, rather than the facilitator. The discussion can be guided by the case itself and the trainees’ questions and answers. A mixed approach to case discussion will provide the variety that the participants value.Although traditionally MR has focused on the diagnosis or management of one or more clinical cases from the inpatient service, its format is flexible enough to provide opportunity for discussion or for other important aspects of patient care. MR can also address ambulatory cases,7 include the presence of a real patient for the purposes of highlighting history-taking and clinical findings and also incorporate discussion of simulated cases, such as code blue scenarios. The discussion can also be enriched by the health professionals from other disciplines including, pharmacists, physical therapists, occupational therapists, nurses, and social workers. The case can also be selected to allow the discussion to be focused on other specific elements of management, such as resource utilization and “choosing wisely,”8 quality and safety, bioethics, and evidence-based medicine.9Running the Show In developing their skills in facilitation, many of the faculty surveyed discussed that they continuously build on the facilitation skills that they have learned over time, the basic principles of which are described elsewhere.10,11 Through feedback and reflection, they adapt to a style that reflects how they believe the MR should be conducted.The facilitator must establish a respectful climate at MR that is conducive to learning. He or she must ensure that the session is collegial and enforce that the goal of the session is learning, rather than showmanship. The environment should encourage interaction and permit people to ask questions. Trainees should feel comfortable enough to answer questions and test hypotheses, even if answers are incorrect. However, the facilitator must ensure that the correct information is conveyed to the group and that incorrect answers are explored as key teaching points. Humour can put people at ease. Self-deprecating humour can be non-threatening and freely employed if it is within the facilitator’s comfort zone. However, humour should never come at the expense of a trainee. Personal anecdotes and reflections on past cases can engage the audience, relax the atmosphere and vividly impart key facts and clinical wisdom.It is important for the facilitator to be respectful of time. Trainees often report that too much time is spent on reviewing the history and physical examination and on the development of an exhaustive differential diagnosis while less time is spent on investigation and management issues, which senior trainees find most valuable. There need not be a fixed formula related to how much time to spend on specific components of the case. A skilled facilitator will expand and abbreviate aspects of the case discussion based on the specific case presented. Some cases represent excellent opportunities to review evidence-based physical examination, some may highlight issues of resource stewardship related to investigation and some are particularly well-suited to discussion of evidence-based management.The facilitator should facilitate a clinical discussion, rather than deliver a didactic talk. He or she should coach the audience to identify key historical facts or findings on physical examination to allow everyone to fully participate in the case formulation and clinical reasoning that will follow. Demonstrating a mix of pattern recognition and heuristics (e.g., “Quick – what do you think the diagnosis is?”) and analytical reasoning strategies will help trainees learn to employ and recognize the strengths and limitations of each.In the face of a quiet audience, questions that have obvious answers should be posed first. The facilitator should promote volunteerism as much as possible; however, addressing specific members of the audience prevents silence and can help ensure everyone is engaged in the discussion. Sensitivity to the level of trainee is important. A facilitator should avoid potential embarrassment of a trainee by allowing a more junior learner to come up with the answer to a question that the more senior trainee could not answer. In other words, there should be an inviolate sequence wherein, for any given topic, the facilitator starts with trainees at an appropriate level for the questions and moves upward sequentially by level of training. This allows participants to relax and set their focus on learning, rather than avoiding eye contact and fearing embarrassment.A skilled facilitator should not allow any one person to dominate the discussion and should also refrain from asking multiple questions to the same participant. However, it can be valuable to challenge a respondent or the group to elaborate on their answers, as this can uncover gaps in knowledge and understanding and provide additional opportunities for learning.It is important to ensure that the discussion is of interest to trainees at all levels. If faculty are present, their opinions should be sought throughout the case. It is helpful to highlight the variability in approach amongst “the experts” in the room. Judicious use and justification of investigations should be encouraged to promote learning about resource stewardship and evidence-based medicine principles should be incorporated, when relevant.Many facilitators are anxious about how to handle situations where they don’t know the answer to a particular clinical problem. In these cases, a demonstration of the clinical reasoning process and a focus on an approach to clinical problems can be helpful. Some of the most useful discussions centre on how to deal with uncertainty and on how to find answers to clinical questions in real-time using available resources. The facilitator should not hesitate to say “I don’t know,” as this demonstrates that nobody has infinite knowledge and role-models the necessity of recognizing one’s limitations. Teaching scripts relating to specific topics or the use of an etiologic or body systems-based approach to developing a differential diagnosis are helpful teaching approaches6.Wrapping Up Sufficient time should be dedicated to recapitulation and repetition of one to 3 key take home messages. This serves to reinforce the important points that were discussed and to ensure that participants walk away with key messages to facilitate learning. Having a few members of the audience identify what they have learned is often beneficial as the facilitator may not identify the same issues as the trainees.Closing the Loop Further reinforcement can occur if a summary of the take home points, or a relevant paper, is circulated by email or posted to a blog.12 This must be done in a manner that protects patient confidentiality. Updates on previously presented diagnostic dilemmas will enhance learning. Finally, the organizer of MR can keep a log of cases that have been presented to avoid excessive repetition of topics and ensure a balanced curriculum.A process for the person presenting the case to be provided with feedback about their presentation skills by the facilitator or peers should be implemented. It is also important for the facilitator to receive feedback about their teaching and the session overall. Feedback will help faculty refine their facilitation skills, especially if coupled with faculty development initiatives to improve teaching skills.13 It may also be important for novice clinician teachers who need to build a teaching portfolio as part of their academic review and promotion process. 14 If it is clear the faculty utilize the feedback, it serves to role-model self-reflection and promote a culture of frequent formative feedback.The Future of MR MR has a long tradition and can be an evolving teaching format capable of meeting current educational needs. For example, with the implementation of competency-based medical education (CBME) into residency training programs, the competencies being developed for Internal Medicine trainees can provide a framework to organize aspects of learning experiences, including MR. 15 Issues of advocacy and stewardship may be highlighted as explicit learning points of cases, as MR allows for discussion of authentic core clinical tasks and problems, avoiding the reduction of competencies to endless lists taught without the necessary context needed for deeper learning.16 There are also challenges to implementing and sustaining a successful MR in today's current training climate. Issues such as duty-hour restrictions, increased volume and acuity of patients, and pressure to discharge patients early in the day17–19 have prompted some to modify the traditional MR. An “afternoon report” allows for attention to clinical duties early in the day and preserves teaching for later in the day. MR should continue to evolve to meet current education and healthcare delivery needs, and these innovations should be described in the literature and studied.Although these tips have been generated from shared experiences at a single centre, we believe they will be useful to facilitators in many other settings, as they represent the experiences of many facilitators with many cumulative years of experience. This article is intended to stimulate others to reflect upon and discuss what they have found to be the key elements to facilitating a successful MR.Acknowledgements We would like to thank our colleagues who contributed tips and whose teaching has influenced the careers of countless trainees: Dr. Ahmed Bayoumi, Dr. Isaac Bogoch, Dr. Mark Cheung, Dr. Allan Detsky, Dr. Irfan Dhalla, Dr. Vera Dounaevskaia, Dr. Trevor Jamieson, Dr. Lauren Lapointe Shaw, Dr. Jerome A. Leis, Dr. Don Livingstone, Dr. Julia Lowe, Dr. Ophyr Mourad, Dr. Valerie Palda, Dr. Joel Ray, Dr. Donald Redelmeier, Dr. Steve Shadowitz, Dr. Rob Sargeant.References1. Parrino TA, Villanueva AG. The principles and practice of MR. JAMA 1986;256(6):730–33.2. Amin Z, Guajardo J, Wisniewski W, Bordage G, Tekian A, Niederman LG. MR: focus and methods over the past three decades. Acad Med 2000;75(10):S1–S5.3. Gross CP, Donnelly GB, Reisman AB, Sepkowitz KA, Callahan MA. Resident expectations of MR: a multi-institutional study. Arch Int Med 1999;159(16):1910–14.4. McNeill M, Ali SK, Banks DE, Mansi IA. MR: can an established medical education tradition be validated? J Grad Med Educ 2013;5(3):374–84.5. Ways M, Kroenke K, Umali J, Buchwald D. MR: A survey of resident attitudes. Arch Int Med 1995;155(13):1433–37.6. Sacher AG, Detsky AS. Taking the stress out of MR: an analytic approach to the differential diagnosis. J Gen Intern Med 2009;24(6):747–51.7. Wenderoth S, Pelzman F, Demopoulos B. Ambulatory MR. J Grad Med Educ 2002;17(3):207–209.8. Kane GC, Holumzer C, Sorokin R. Utilization management MR: Purpose, planning and early experience in a university hospital residency program. Sem Med Pract 2001;4(1):27–36.9. Banks DE, Runhua Shi M. Decreased hospital length of stay associated with presentation of cases at MR with librarian support. J Med Libr Assoc 2007;95(4):381–87.10. Azer SA. Challenges facing PBL tutors: 12 tips for successful group facilitation. Med Teach 2005;27(8):676–81.11. Skeff KM. Enhancing teaching effectiveness and vitality in the ambulatory setting. J Gen Intern Med 1988;3(1):S26–S33.12. Bogoch II, Frost DW, Bridge S, Lee TC, Gold WL, Pansiko DM, Cavalcanti R. MR blog: a web-based tool to enhance case-based learning. Teach Learn Med 2012;24(3):238–41.13. Boerboom TB, Stalmeijer RE, Dolmans DH, Jaarsma DA. How feedback can foster professional growth of teachers in the clinical workplace: A review of the literature. Stud Educ Eval 2015;46:47–52.14. Fleming VM, Schindler N, Martin GJ, DaRosa DA. Separate and equitable promotion tracks for clinician-educators. JAMA 2005;294(9):1101–1104.15. Frank JR, Snell LS, Ten Cate O, Holmboe ES, Carraccio C, Swing SR, Harris, KA. Competency-based medical education: theory to practice. Med Teach, 2010;32(8):638–45.16. Hawkins RE, Welcher CM, Holmboe ES, Kirk LM, Norcini JJ, Simons KB, Skochelak SE. Implementation of competency‐based medical education: are we addressing the concerns and challenges? Med Educ. 2015;49(11):1086–1102.17. Arora VM, Georgitis E, Siddique J, Vekhter B, Woodruff JN, Humphrey HJ, Meltzer DO. Association of workload of on-call medical interns with on-call sleep duration, shift duration, and participation in educational activities. JAMA 2008;300(10):1146–53.18. Horwitz LI, Krumholz HM, Huot SJ, Green ML. Internal medicine residents' clinical and didactic experiences after work hour regulation: a survey of chief residents. J Gen Int Med 2006;21(9):961–65.19. Khanna S, Sier D, Boyle J, Zeitz K. Discharge timeliness and its impact on hospital crowding and emergency department flow performance. Emerg Med Aus 2016;28(2):164–70.

Introduction The study of online conspiracy theory communities presents unique methodological challenges. Online conspiracy theorists often adhere to an individualistic knowledge culture of “doing one’s own research” (Fenster 158). This results in a decentralised landscape of theories, narratives and communities that challenges conventional top-down approaches to analysis. Moreover, conspiracy theories tend to be discussed on the fringes of the online ecosystem, in chat groups, small subcultural Web forums and away from mainstream social media platforms such as Facebook and Twitter (Frenkel; see also De Zeeuw et al.). In this context, the messaging app Telegram has developed into a particularly prominent space (Rogers, “Deplatforming”; Urman and Katz). On the one hand, this platform is not quite part of the same mainstream as Facebook or Twitter, owing in part to its emphasis on security, “social privacy”, and lack of central moderation (Rogers, “Deplatforming”). But it is also not quite an “alternative social medium” (Gehl), as it does not position itself in opposition to mainstream platforms per se, nor does its business model centered around investor funding and advertisements present a break from the “dominant political economy” (ibid.). This ambiguous position might account for Telegram’s wide adoption, as well as its status as a relatively safe haven for communities deplatformed elsewhere – including a lively ecosystem of conspiracy theory communities (La Morgia et al.). Because Telegram communities are distributed over a wide range of channels and chat groups, they cannot always be investigated using existing analytical approaches for social media research. Confronting this challenge, we propose and discuss a method for studying Telegram communities that repurposes the “methods of the medium” (Rogers, Digital Methods). Specifically, our method appropriates Telegram’s feature of forwarding messages from one group to another to discover interlinked distributed communities, collect data from these communities for close reading, and map their information sharing practices. In this article, we will first present this approach and illustrate the types of analyses the collected data might afford in relation to a brief case study on Dutch-speaking conspiracy theories. In this short illustration, we map the convergence of right-wing and conspiratorial communities, both structurally and discursively. As Vieten discusses, “digital pandemic populism during lockdown might have pushed further the mobilisation of the far right, also on the streets”. In the Dutch context there has been a demonstrated connection between the two. Because of this connection, we were drawn to the questions of what these entanglements might look like in a relatively unmoderated Telegram environment. We then proceed to discuss some strengths and limitations, identify avenues for future research, and conclude with some ethical, methodological and epistemological reflections. Overview of Method Our method first combines expert knowledge, and the affordances of the Telegram app’s ‘search’ function to retrieve a set of channels mentioning specific politicians or political parties, as well as other marked terms that might point towards far-right or conspiratorial content. This includes wakker (awakened), variations of batavier and geus (nationalist demonyms), names of known far right politicians (such as The Netherlands’ Thierry Baudet and Flanders’ Dries Van Langenhove) and conspiracy theory activists, and volk (a term meaning roughly “our people”). As this approach precludes discovery of related groups that do not match the queries exactly, this initial curated list is then supplemented with channels advertised elsewhere, such as those featured on the Websites of far-right politicians and organisations, as well as channels covered in mainstream news media. This yields an initial expert list of channels, in our sample case of Dutch-speaking right-wing and conspiracist actors comprising 50 items. One might stop here, and collect data for this manually curated list of groups, as in Nikkhah et al.’s study of Telegram use among the Iranian diaspora in the United States, or Davey and Weinberg’s analysis of far-right groups used in the US military. But this would exclude any groups not known by the researchers; and groups are not always easy to naively discover on Telegram. Because of this, in a subsequent step, we expanded the initial set of relevant Telegram channels by crawling posts in these channels that were forwarded from other channels, constituting links between these channels. We used a custom crawler based on the open source library Selenium, which allows one to control a browser programmatically. The browser was then made to scroll through the Web-based view of the selected channels (e.g. https://t.me/s/durov). In principle, all messages ever posted in a channel are available in this view. We then follow those links, and store the names of the linked channels. Overall, this method thus presumes that if a channel forwards a message from another channel there will be some overlap in terms of topic of discussion between both, making the newly discovered channel similarly relevant to the analysis. This results in a network-like representation of connections between channels. In the context of our case study, this process expands our initial seed list to a list of over 215 relevant public channels, after discarding groups that are not germane to the case study, i.e. those that were not related to far-right or conspiracy theory-related communities. To verify this, channels were inductively coded by a team of four researchers after capture. We then repeat the data collection for this new list of channels, retrieving forwarded messages from over 370,000 total messages spanning the period 2017-2021. This dataset then serves as a starting point for structural analysis of the wider context of the community, aspects of which will be illustrated in the next section. Illustration Emphasising the value of a “quali-quanti” (Venturini and Latour) approach we offer a tentative analysis of the decentralised Dutch-speaking conspiracist narratives and communities on Telegram, and in a broader sense observe what such a distributed community may look like on the platform. This then suggests the various affordances which a dataset collected with this method can offer. Fig. 1: Network visualisation of collected channels (depth: 1) including channels forwarded from (4354 nodes). Nodes are sized and coloured by degree (amount of connections to other channels). A first observation that can be made concerns the topology of the network of channels we found (see fig. 1). A network analysis is a suitable distant reading approach for this kind of data, because it “maps and measures formal and informal relationships … viz., who knows whom, and who shares what information and knowledge with whom” (Serrat 40). It is a type of analysis that can reveal the relevance and positioning of actors and narratives within the data. In our network visualisation, we use the ForceAtlas2 algorithm (Jacomy et al.) to position the nodes. This algorithm makes more connected nodes “gravitate” towards each other; the more central a node is, the more connected it is to the rest of the network, roughly speaking (Figure 1). Highlighting the channels representing political parties shows, for example, that while the Dutch far right party FvD (FVDNL) is quite central (connected), this is not the case for the Flemish far right politician Dries Van Langenhove (kiesdries). This suggests that compared to a similar Flemish politician, the Dutch FvD is a more prominent part of the general conspiracist discussion – which is then perhaps more overtly politicised in the Dutch context. We can additionally discern channels that we might label “content aggregators”, which forward large numbers of messages from other channels but post comparatively little original content, occupying a relatively central place in the wider network. These content aggregators play an important structural role in the network, as other channels might forward messages from these collections on a “pick and choose” basis. More abstractly, they also serve to confirm thematic similarity between the channels messages are forwarded from, with the owners of the aggregator channels playing the role of a curator that collects interesting content about a certain topic of interest. Furthermore, our data reveal that the network is highly dynamic. As forwarded messages are timestamped, we can plot the graph at different moments in time. When comparing changes over a year, we can observe a significant growth in the number of channels that connect to the network particularly between 2020 and 2021 (see fig. 2). This growth, and the associated diversity of the network, can be attributed to Telegram’s role as a haven for actors that were deplatformed (or present themselves as targets of deplatforming and censorship) from other social media; a “platform of last resort”. Previous research has for instance indicated that a number of alt-right fringe actors moved to Telegram after being deplatformed from Facebook or Twitter (Rogers, “Deplatforming”). It can be hypothesised that events such as Donald Trump’s removal from Twitter around the time of the January 2021 Capitol riots might similarly have inspired other actors to move to Telegram in response to the platform policies of mainstream social media. Fig. 2: The channel network based on messages sent before June 2020 (334 nodes). Same layout and parameters as in Figure 1 (not to scale). Nodes also appearing in Figure 1 are highlighted. The structure of the network (in fig. 1) can also be used to discern ‘sub-communities’, which forward messages mutually but have relatively few links to the broader network. These can then be analysed qualitatively. This may then reveal that Flemish groups that oppose COVID-19 policies are less connected with the far right, whereas such groups that can be identified as Dutch seem to merge more easily with far-right channels. As discussed, this is also suggested by the position of FVDNL, the Dutch far-right political party Forum voor democratie (FvD) which is central in the network. On this level, this suggests that structurally, Dutch far-right parties are more explicitly affiliating themselves with conspiracy-related channels than Flemish parties. Actual textual analyses of the channels’ posts and images, however, could offer a more nuanced picture, whereby structurally unconnected channels might still share common harmful narratives, spanning anti-progressive discourse, anti-mainstream sentiments, anti-government discourse, and evocations of prominent conspiracy theories such as QAnon and The Great Reset. These structural analyses then present a number of possibilities for further content analysis, where one might for example “zoom in” on Dutch far-right groups in particular, and qualitatively study images posted therein to identify salient narratives and positions. Discussion Methodological Gains Variations of the proposed approach have been used in other work (e.g. Hashemi and Chahooki; La Morgia et al.). Most prominently, the Pushshift Telegram Dataset (Baumgartner et al.) comprises a large dataset of channel metadata, author metadata and messages. This dataset was collected by discovering new channels from an initial seed list of approximately 300 channels using forwarded messages, and then collecting messages from these channels. While there is great archival value in the resulting datasets, our approach differs from these earlier approaches in a number of instructive ways. Like other work we appropriate an affordance of Telegram – forwarded messages – for our own research purposes, but we purposely limit the extent of “following” these forwarded messages. Though one could keep following links indefinitely, not every link is a link that is structural to the distributed community of users that is of interest here. Though more extensive crawling might reveal ever more channels and associated data, these are also increasingly unlikely to be related to the initial topic of interest, and are in any case further removed from the users of the initial seed groups. For this reason, we use a relatively shallow crawl depth and only retain links up to two “hops” away from the initial seed channels. This trades a higher number of crawled channels for a higher likelihood that the captured channels are indeed relevant to the case study. The suitable crawl depth would differ from case to case. In our case, it was established empirically through pilot crawls, which were stopped once collected groups appeared to no longer be strongly connected to the initial seed groups by topic. Datasets of this type are often also difficult to reproduce or qualify. For example for the datasets compiled by La Morgia et al. as well as for Baumgartner et al., the original seed list is not provided. Because of this, it is impossible to see where the network of found groups originates and how it might be biased one way or another. We suggest that where possible, this seed list is documented and shared. In our case, this would be particularly important as the seeds represent an intentional and explicit bias; that is, Dutch-speaking conspiracy-themed and far-right groups. If the starting point of the crawl is documented, one could potentially re-collect the data later from the same starting points and compare results to those found initially, allowing for longitudinal analyses of the topology of these communities. Ethics The method described here does not deal with personally identifiable information (PII) per se; one can map the channel network on a structural level without collecting user data or analysing specific messages, when purely tracing the origin of forwarded messages. It should be noted, however, that in the process of collecting these structural data, one can potentially go further. For example, it is possible to scrape the full content of messages. When also including chat groups, user details including (user-provided) full names are also available. Their inclusion in (public) datasets should be subject to closer scrutiny than that of public channels, as the former may represent conversations had under the assumption that this conversation was more or less off the record; while the latter are explicitly intended for information broadcast. Even if many of these chat groups are technically public, we should consider that "even if users are aware of being observed by others, they do not consider the possibility that their actions and interactions may be documented and analysed in detail at a later occasion" (Sveningsson Elm 77). In many cases, a (structural) analysis of only channels strikes a good balance between collecting representative data and respecting the privacy of those who produce the collected data. Avenues for Future Research The method may be expanded in a number of ways. One could, as discussed, increase the amount of crawl iterations, which would expand the network at the potential cost of case-specificity. A larger seed list could also increase the quantity of the data, though the effect of this can often be limited, as a relatively limited amount of channels forward messages from other channels. Links between channels could be collected not only from forwarded messages, as we do here, but also via other repurposed Telegram features such as channel invitation links or simple hyperlinks to other channels found in message content. The latter would require more fine-grained parsing of the message texts through natural language processing, for example, as a hyperlink can suggest a wider range of connections than an intentionally forwarded message. Additionally, and as previously mentioned, one could include not only public channels but also public chat groups, which are often linked to these channels and offer a space for people to discuss the content posted in them. While this can be an attractive way of acquiring extra data, we forego this in our example. As discussed, there are ethical trade-offs to consider when deciding to work with data from groups; but, it can be argued that Telegram channels represent an explicit “broadcast” style of communication (Shehabat et al.). Because the channel owner(s) decide what is worthy of sharing, one can reasonably assume that if one “follows the medium” here, all content retrieved from a channel will be somewhat relevant to the channel's purported theme. Conversely, discourse in chat groups might be expected to meander into a variety of directions and can easily include many (forwarded) messages only tangentially related to the case study of interest. Conclusion In this article we have sought to present one methodological approach to studying communities on Telegram. Rather than presenting a thorough case study or a definitive analysis of the Telegram-based community we discuss, our goal was to demonstrate the method's benefits but also its potential shortcomings, avenues of further development, and what types of analysis data collected with it might afford. A cursory analysis of the fringe community we studied here shows how with such data one can map a given community or set of communities on a structural level, which may then be used to demarcate areas of interest for further content analysis. The observations presented in this article are far from a complete picture of the data collected, but can serve as suggestions for analytical avenues one might venture down in a more substantive analysis. Beyond these observations, our repurposing of “the methods of the medium” (Rogers, Digital Methods) through forwarded messages allows us to contribute an empirically informed reflection on the possibilities and limitations of studying conspiracist information sharing practices on Telegram. Our method for instance highlights tensions between public and private knowledge, whereby we only consider information from public channels, and for technical and ethical reasons omit Telegram’s closed-off, private chat groups from our analysis. Our method of sourcing channels through forwarded messages does not preclude the existence of isolated channels or clusters of channels that, for a lack of forwarded messages from channels that were already identified, elude the scope of such snowballing efforts. Along the same lines, one could imagine that a deeper, more far-reaching crawl would reveal some strange bedfellows for the initial seed that were not part of our a priori understanding and hypotheses concerning the communities of interest. All of these considerations represent choices that may be taken differently depending on the case study at hand. Statement on Data and Ethics The analysis on offer in this article is limited to names of public channels on Telegram and we purposely refrain from citing channel names or analysing specific messages so they cannot be traced back to single persons. Our analysis does not comprise live subjects or PII, and thus did not require ERB clearance from our respective institutions. The anonymised dataset described above is available upon request via Zenodo at https://doi.org/10.5281/zenodo.6344795. 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