Статті в журналах: "Fish cell line"

1

Kolarova, J., J. Velisek, and Z. Svobodova. "Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology." Veterinární Medicína 66, No. 8 (July 5, 2021): 350–55. http://dx.doi.org/10.17221/161/2020-vetmed.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The use of in vitro (fish cell lines) is a cost-effective, very rapid, and informative tool for toxicological assessments. Using the neutral red (NR) assay, we compared the in vitro acute toxicity (20hEC50) of twenty-six chemical substances on a rainbow trout gonad cell line (RTG-2) with their in vivo acute toxicity to Barbados Millions Poecilia reticulata (48hLC50, OECD 203) and crustacean Daphnia magna (48hEC50, OECD 202). The 20hEC50 values obtained by the NR assay were higher in nearly all the cases when compared to the 48hLC50 in P. reticulata and the 48hEC50 in D. magna, indicating that the sensitivity of the RTG-2 cell line was lower compared to P. reticulata and D. magna. A high (r = 0.89) and significant (P < 0.001) correlation was recorded between the 20hEC50 values of the RTG-2 and the 48hEC50 values of D. magna. The correlation between the 20hEC50 values of the RTG-2 and the 48hLC50 values of P. reticulata was lower (r = 0.65; P < 0.001), but also significant. The authors recommend use of the NR assay on the RTG-2 cell lines as a screening protocol to evaluate the toxicity of xenobiotics in aquatic environments to narrow the spectrum of the concentrations for the fish toxicity test.

2

Kan, Yuting, Ying Zhong, Muhammad Jawad, Xiao Chen, Dong Liu, Mingchun Ren, Gangchun Xu, Lang Gui, and Mingyou Li. "Establishment of a Coilia nasus Gonadal Somatic Cell Line Capable of Sperm Induction In Vitro." Biology 11, no. 7 (July 13, 2022): 1049. http://dx.doi.org/10.3390/biology11071049.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Coilia nasus is an important economic anadromous migratory fish of the Yangtze River in China. In recent years, overfishing and the deterioration of the ecological environment almost led to the extinction of the wild resources of C.nasus. Thus, there is an urgent need to protect this endangered fish. Recently, cell lines derived from fish have proven a promising tool for studying important aspects of aquaculture. In this study, a stable C. nasus gonadal somatic cell line (CnCSC) was established and characterized. After over one year of cell culture (>80 passages), this cell line kept stable growth. RT-PCR results revealed that the CnGSC expressed some somatic cell markers such as clu, fshr, hsd3β, and sox9b instead of germ cell markers like dazl, piwi, and vasa. The strong phagocytic activity of CnGSC suggested that it contained a large number of Sertoli cells. Interestingly, CnGSC could induce medaka spermatogonial cells (SG3) to differentiate into elongated spermatids while co-cultured together. In conclusion, we established a C. nasus gonadal somatic cell line capable of sperm induction in vitro. This research provides scientific evidence for the long-term culture of a gonadal cell line from farmed fish, which would lay the foundation for exploring the regulatory mechanisms between germ cells and somatic cells in fish.

3

Lee, K. W., S. C. Chi, and T. M. Cheng. "Interference of the life cycle of fish nodavirus with fish retrovirus." Journal of General Virology 83, no. 10 (October 1, 2002): 2469–74. http://dx.doi.org/10.1099/0022-1317-83-10-2469.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Interference of the life cycle of grouper nervous necrosis virus (GNNV), a member of the Nodaviridae, genus Betanodavirus, by snakehead retrovirus (SnRV) has been studied in vitro. SGF-1, a new fish cell line that is persistently infected with SnRV, was induced by inoculating SnRV into the grouper fin cell line GF-1. Culture supernatants and cell pellets from both GNNV-infected SGF-1 and GF-1 cells were collected and employed for virus productivity analysis. The yields of GNNV RNA and capsid protein in GNNV-infected SGF-1 cells were similar to those in GNNV-infected GF-1 cells. However, when GF-1 cells were used for titration, the titre of the culture supernatant from GNNV-infected SGF-1 cells was much higher than that from GNNV-infected GF-1 cells. The titration result suggested that SnRV enhanced the infection or cytopathic effect (CPE) of GNNV during GNNV and SnRV coinfection of the GF-1 cell titration system, although SnRV cannot induce any CPE in GF-1 cells alone, nor can it increase the yield of GNNV after GNNV superinfection of SGF-1 cells. Moreover, GNNV cDNA was detected in both the pellet and the supernatant from GNNV-infected SGF-1 cells. This result indicated that SnRV reverse-transcribed the GNNV single-stranded genomic RNA into cDNA during GNNV superinfection of SGF-1 cells and created a new cDNA stage in the life cycle of the fish nodavirus.

4

Tanneberger, Katrin, Melanie Knöbel, Frans J. M. Busser, Theo L. Sinnige, Joop L. M. Hermens, and Kristin Schirmer. "Predicting Fish Acute Toxicity Using a Fish Gill Cell Line-Based Toxicity Assay." Environmental Science & Technology 47, no. 2 (December 27, 2012): 1110–19. http://dx.doi.org/10.1021/es303505z.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Tung, Li-Chu, Yung-Reui Chen, Shiu-Nan Chen, and Guang-Hsiung Kuo. "Fish kidney cell line in response to heat shock." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 1 (August 1992): 704–5. http://dx.doi.org/10.1017/s0424820100123921.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

6

Gagné, F., and C. Blaise. "Evaluation of environmental estrogens with a fish cell line." Bulletin of Environmental Contamination and Toxicology 65, no. 4 (October 2000): 494–500. http://dx.doi.org/10.1007/s001280000151.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Chaumont, L., L. Jouneau, M. Peruzzi, P. Boudinot, and B. Collet. "Functional characterisation of a Viperin knockout fish cell line." Developmental & Comparative Immunology 148 (November 2023): 104932. http://dx.doi.org/10.1016/j.dci.2023.104932.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Zhang, Hetong, Junjian Dong, Yunyun Yan, Shanshan Liu, Xing Ye, Fengying Gao, and Chengfei Sun. "Development of a Highly Permissive Mandarin Fish (Siniperca chuatsi) Kidney Cell Line for Mandarin Fish Ranavirus Using a Single-Cell Cloning Method." Cells 13, no. 1 (December 20, 2023): 18. http://dx.doi.org/10.3390/cells13010018.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Mandarin fish ranavirus (MRV) infection poses a substantial challenge to the mandarin fish culture industry as no effective preventive or therapeutic measures currently exist. The creation of a highly permissive cell line from a natural host is crucial for developing a vaccine for MRV and understanding its pathogenic mechanisms. In this research, the mandarin fish (Siniperca chuatsi) kidney cell line (SCK) was isolated from mandarin fish kidneys. Subsequently, SCK-a to SCK-g monoclonal cell lines were derived from the SCK cell population, distinguished by morphological variations. Notably, MRV infection induced an advanced cytopathic effect (CPE) in almost all cells of the SCK-f clone. Further tests showed that MRV achieved a peak viral titer of 1010.7 50% tissue culture infectious dose (TCID50)/mL and consistently exceeded 1010 TCID50/mL across nine passages in SCK-f cells. Electron microscopy verified the MRV virion integrity within SCK-f. In vivo experiments revealed that MRV infections led to cumulative mortality rates of 86.9% in mandarin fish and 88.9% in largemouth bass (Micropterus salmoides). Such results suggest that SCK-f is highly permissive to MRV. This study underscores the importance of cellular diversity in developing viral permissive cell lines. The SCK monoclonal cell line pool may offer potential for generating highly permissive cell lines for other mandarin fish viruses.

9

Luo, Xia, Xiaozhe Fu, Min Zhang, Hongru Liang, Yinjie Niu, Qiang Lin, Baofu Ma, Lihui Liu, and Ningqiu Li. "Development of a New Marine Fish Continuous Cell Line Derived from Brain of Red Sea Bream (Pagrosomus major) and Its Application to Fish Virology and Heavy Metal Toxicology." Animals 13, no. 22 (November 15, 2023): 3524. http://dx.doi.org/10.3390/ani13223524.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Red sea bream (Pagrosomus major) is one of the most popular farmed marine teleost fish species. Fish cell lines are becoming important research tool in the aquaculture field, and they are suitable models to study fish virology, immunology and toxicology. To obtain a Pagrosomus major cell line for biological studies, a continuous cell line from brain of red sea bream (designated as RSBB cell line) was established and has been successfully subcultured over 100 passages. The RSBB cell line predominantly consisted of fibroblast-like cells and multiplied well in M199 medium supplemented with 10% fetal bovine serum at 28 °C. Karyotyping analysis indicated that the modal chromosome numbers of RSBB cells was 48. After transfection with pEGFP-N1, RSBB cells showed bright green fluorescence with a transfection efficiency approaching 8%. For toxicology study, it was demonstrated that metal Cd could induce cytotoxic effects of RSBB cells, accompanied with a dose-dependent MTT conversion capacity. Morphologically, cells treated with metal Cd produced rounding, shrinking and detaching and induced both cell apoptosis and necrosis. For virology study, the RSBB cells were highly susceptible to Nervous necrosis virus (NNV) and Singapore grouper iridovirus (SGIV) with steady titers (i.e., 108.0~8.3 TCID50 mL−1 and 107.0~7.2 TCID50 mL−1 respectively). Furthermore, an obvious cytopathic effect (CPE) could be observed in RSBB cells infected with Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdoviruses (SCRV). Meanwhile, all the infections were confirmed by polymerase chain reaction. The new brain cell line developed and characterized from red sea bream in this study could be used as an in vitro model for fish studies in the fields of toxicology and virology.

10

TAKARADA, Yutaka, Yoshio OJIMA, and Akinori TAKAI. "Establishment of a fish cell line transformed by MNNG, RFM." Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 65, no. 5 (1989): 108–11. http://dx.doi.org/10.2183/pjab.65.108.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

11

WANG, Jiyang, and Yoshio OJIMA. "Isolation and characterization of a BUdR-resistant fish cell line." Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 65, no. 5 (1989): 112–15. http://dx.doi.org/10.2183/pjab.65.112.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Frerichs, G. N. "Identification and elimination of mycoplasmas in fish cell line cultures." Journal of Fish Diseases 19, no. 6 (November 1996): 435–39. http://dx.doi.org/10.1046/j.1365-2761.1996.00258.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Frerichs, G. N. "Identification and elimination of mycoplasmas in fish cell line cultures." Journal of Fish Diseases 19, no. 6 (November 1996): 435–39. http://dx.doi.org/10.1111/j.1365-2761.1996.tb00383.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Bandín, I., J. G. Olveira, J. J. Borrego, C. P. Dopazo, and J. L. Barja. "Susceptibility of the fish cell line SAF-1 to betanodavirus." Journal of Fish Diseases 29, no. 10 (October 2006): 633–36. http://dx.doi.org/10.1111/j.1365-2761.2006.00757.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Sapède, Dora, Nicolas Gompel, Christine Dambly-Chaudière, and Alain Ghysen. "Cell migration in the postembryonic development of the fish lateral line." Development 129, no. 3 (February 1, 2002): 605–15. http://dx.doi.org/10.1242/dev.129.3.605.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

We examine at the cellular level the postembryonic development of the posterior lateral line in the zebrafish. We show that the first wave of secondary neuromasts is laid down by a migrating primordium, primII. This primordium originates from a cephalic region much like the primordium that formed the primary line during embryogenesis. PrimII contributes to both the lateral and the dorsal branches of the posterior lateral line. Once they are deposited by the primordium, the differentiating neuromasts induce the specialisation of overlying epidermal cells into a pore-forming annulus, and the entire structure begins to migrate ventrally across the epithelium. Thus the final two-dimensional pattern depends on the combination of two orthogonal processes: anteroposterior waves of neuromast formation and dorsoventral migration of individual neuromasts. Finally, we examine how general these migratory processes can be by describing two fish species with very different adult patterns, Astyanax fasciatus (Mexican blind cavefish) and Oryzias latipes (medaka). We show that their primary patterns are nearly identical to that observed in zebrafish embryos, and that their postembryonic growth relies on the same combination of migratory processes that we documented in the case of the zebrafish.

16

Chen, Wenbiao, Shawn Burgess, Greg Golling, Adam Amsterdam, and Nancy Hopkins. "High-Throughput Selection of Retrovirus Producer Cell Lines Leads to Markedly Improved Efficiency of Germ Line-Transmissible Insertions in Zebra Fish." Journal of Virology 76, no. 5 (March 1, 2002): 2192–98. http://dx.doi.org/10.1128/jvi.76.5.2192-2198.2002.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

ABSTRACT Vesicular stomatitis virus glycoprotein G-pseudotyped mouse retroviral vectors have been used as mutagens for a large-scale insertional mutagenesis screen in the zebra fish. To reproducibly generate high-titer virus stocks, we devised a method for rapidly selecting cell lines that can yield high-titer viruses and isolated a producer cell line that yields virus at a high titer on zebra fish embryos. Virus produced from this line, designated GT virus, is nontoxic following injection of zebra fish blastulae and efficiently infects embryonic cells that give rise to the future germ line. Using GT virus preparations we generated roughly 500,000 germ line-transmissible proviral insertions in a population of 25,000 founder fish in about 2 months. The GT virus contains a gene trap, and trap events can be detected in the offspring of almost every founder fish. We discuss potential applications of this highly efficient method for generating germ line-transmissible insertions in a vertebrate

17

Swaminathan, T. Raja, A. Gopalakrishnan, V. S. Basheer, P. R. Divya, and J. K. Jena. "Development and characterization of a new fish cell line from Honeycomb grouper, Epinephelus merra." Indian Journal of Animal Sciences 82, no. 9 (September 11, 2012): 1100–1105. http://dx.doi.org/10.56093/ijans.v82i9.23682.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

A new cell line HGF was developed from fin tissue of Epinephelus merra Bloch, 1793. The cell line was maintained in Leibovitz’s L-15 supplemented with 15% FBS and cells have been subcultured 45 times. The HGF cell line consists predominantly of fibroblastic-like cells. The cells were able to grow at temperatures between 25° and 32°C with optimum temperature of 28°C. The growth rate of fin cells increased as the FBS proportion increased from 2 to 20% at 28°C with optimum growth at the concentrations of 15 or 20% FBS. After confluence, the cells were sub-cultured with a split ratio of 1:2. The cells showed fibroblastic-like morphology and reached confluence on the fourth day after subculture. Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI indicated identity of these cell lines with those reported from this animal species, confirming that the cell lines were of honeycomb grouper origin. The cells were successfully cryopreserved and revived at passage numbers 10, 20 and 30. The bacterial extracellular products from Vibrio cholerae MTCC 3904 was found toxic to this cell line.

18

Sindre, Hilde, Mona C. Gjessing, Johanna Hol Fosse, Lene C. Hermansen, Inger Böckerman, Marit M. Amundsen, Maria K. Dahle, and Anita Solhaug. "Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)." Cells 10, no. 9 (September 16, 2021): 2442. http://dx.doi.org/10.3390/cells10092442.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish.

19

Reid, Ross M., Keira Osbourn, Timothy Leeds, and Gregory D. Wiens. "Selection for disease resistance in Oncorhynchus mykissincreases the myeloid to lymphoid balance and is affected by rapamycin as measured by flow cytometry and scRNAseq." Journal of Immunology 210, no. 1_Supplement (May 1, 2023): 225.03. http://dx.doi.org/10.4049/jimmunol.210.supp.225.03.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Abstract Heritable variation in disease resistance has been described in many species, but the effects of selection on innate immunity are unclear. Here, we compare the cellular response of two lines of rainbow trout that differ in survival due to five generations of selection for resistance to Flavobacterium psychrophilum(Fp), an important fish pathogen. We examined bacterial load, plasma biomarkers, whole blood cellular composition, and blood chemistry at days 1 and 5 after bacterial or PBS injection. We also examined the effect of 0.8 mg/kg rapamycin pretreatment, which results in immunosuppression and a ~100-fold reduction in the challenge LD 50. On day 1, bacterial load did not differ between genetic lines and treatment, but by day 5, the R-line exhibited no increase in Fpload, while rapamycin increased R- and S-line loads 33- and 506-fold, respectively. Biomarkers: cathelicidin 2 and C1q-LP3 significantly differed between genetic lines and by time, corresponding to Fpload. Analysis of whole blood by flow cytometry identified a response to infection and an ~8 percentage point higher frequency of myeloid-gated cells in R-line fish that was consistent in both naïve and challenged animals. S-line fish exhibited a higher percentage of IgM +B cells. Single-cell RNAseq was conducted on day 1 purified blood and identified 23 cell clusters including multiple B cell, neutrophil and macrophage subpopulations. In naïve animals, S-line fish harbored a cxcr4and mychexpressing B cell subpopulation absent in R-line fish. Rapamycin pretreatment caused profound loss of multiple B cell subpopulations in R- and S-line fish. In summary, selection of rainbow trout for resistance to Fpis correlated with a cellular shift in peripheral blood and is mTOR dependent. US Department of Agriculture, Agricultural Research Service; Project number 1930-32000-007

20

Campos, Lydia, Andrei Tchirkov, Jerome Cornillon, Mirella Mihaescu, Michel Giollant, Simone Piselli, Philippe Vago, and Michele Cottier. "Evaluation of Focal Adhesion Kinase Gene Expression in Leukemia and Cancer Cell Lines Using Combined Molecular Cytogenetic Investigations and Quantitative Real Time RT-PCR." Blood 104, no. 11 (November 16, 2004): 4282. http://dx.doi.org/10.1182/blood.v104.11.4282.4282.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Abstract FAK (Focal Adhesion Kinase) is a non-receptor tyrosine kinase protein implicated in integrin-mediated signal pathways. The human gene fak is located on chromosome 8q near c-myc. Cells that overexpress FAK show increased migration and increased cell survival with apoptosis inhibition. The aim of the present study was to investigate the genomic or post-genomic origin of this overexpression in seven tumor cell lines with different levels of FAK expression: K562, an erythroblastic cell line; U937, a monoblastic cell line; KG1a, a poorly differenciated myeloblastic cell line; HL 60, a promyelocytic cell line; Jurkat, a lymphoblastic cell line; A549, a bronchial adenocarcinoma cell line; M4Beu, a lymph node metastasis melanoma cell line. Total or partial chromosome 8 qualitative or quantitative abnormalities and the presence of double minute chromosomes bearing coding genomic DNA fragments were investigated using both conventional cytogenetic (standard karyotype after R- and G- banding) and molecular cytogenetic such as multicolor karyotype (M-FISH), FISH and comparative genomic hybridization (CGH). The mRNA expression was investigated using quantitative real time RT-PCR and the protein level using immunocytochemistry and western blot (immunoblot) analysis. Except for the Jurkat cell line, karyotypes revealed complex modifications. FISH and M-FISH allowed to precisely identify the chromosomal origin of the genomic material on each chromosome and CGH allowed to characterize relative loss and gain of coding genomic DNA and to identify their chromosomal origin. Summarized results are shown in the following table: No detectable RT-PCR product was observed for HL60 and U937 cell lines. The preliminary results show that there was usually a good correlation between PCR data and western blot analysis. However, FAK overexpression for KG1a cell line most probably has a genomic origin, while in K562 and A549 cell lines the origin is most probably at the transcription or post-transcription level without any abnormality of chromosome 8. Dysregulation of fak gene is observed in hematopoietic as well as solid tulor cell lines, and may contribute to leukemogenesis.</CENTER> Cell line FISH 8q24-qter CGH Chr. 8 RT-PCR RT-Q-PCR Western blot RT-Q-PCR results in copy number ratio K562 Normal Normal + 130.1 ++++ KG1a Amplified enh(8)(q11qter) + 37.5 ++++ Jurkat Normal Normal + 8.7 + (low) U937 Normal enh(8)(pterqter) 0 0 0 HL60 Amplified amp(8)(q23-24) 0 0 0 M4Beu Normal Normal + 43.5 0 A549 Normal Normal + 46 ++++

21

Liu, Xiaodan, Jiagang Tu, Junfa Yuan, Xueqin Liu, Lijuan Zhao, Farman Dawar, Muhammad Khattak, et al. "Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection." International Journal of Molecular Sciences 17, no. 2 (January 26, 2016): 154. http://dx.doi.org/10.3390/ijms17020154.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

22

McCONNACHIE, S. H., J. SHEPPARD, G. M. WRIGHT, and D. J. SPEARE. "Development of the microsporidian parasite, Loma salmonae, in a rainbow trout gill epithelial cell line (RTG-1): evidence of xenoma development in vitro." Parasitology 142, no. 2 (December 1, 2014): 326–31. http://dx.doi.org/10.1017/s0031182014001620.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

SUMMARYGrowth and propagation of fish-infecting microsporidians within cell culture has been more difficult to achieve than for insect- and human-infecting microsporidians. Fish microsporidia tend to elicit xenoma development rather than diffuse growth in vivo, and this process likely increases host specificity. We present evidence that the fish microsporidian, Loma salmonae, has the capacity to develop xenomas within a rainbow trout gill epithelial cell line (RTG-1). Spore numbers increased over a 4 weeks period within cell culture flasks. Xenoma-like structures were observed using phase contrast microscopy, and then confirmed using transmission electron microscopy. Optimization of the L. salmonae-RTG-1 cell model has important implications in elucidating the process of xenoma development induced by microsporidian parasites.

23

Tan, Leilei, Qian Liu, Yangbin He, Jingjing Zhang, Jilun Hou, Yuqin Ren, Wenxiu Ma, Qian Wang, and Changwei Shao. "Establishment and Characterization of a Spermatogonial Stem Cell Line from Tiger Puffer Fish (Takifugu rubripes)." Animals 13, no. 18 (September 19, 2023): 2959. http://dx.doi.org/10.3390/ani13182959.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Tiger puffer fish (Takifugu rubripes) has become the main fish species cultured in China since the last century because of its high economic value. Male and female tiger puffer fish need 2 and 3 years each to reach sexual maturity, which limits the development of breeding research for this species. In recent years, in vitro culture of fish spermatogonial stem cells (SSCs) have shown potential in aquaculture. In the present study, we established a spermatogenic stem cell line from T. rubripes (TrSSCs). TrSSCs were characterized by polygonal morphology, predominantly retained 44 chromosomes, and grew rapidly at 26 °C and in L-15. TrSSCs were still able to grow stably after more than one year of in vitro culture. TrSSCs showed positive alkaline phosphatase staining. TrSSCs expressed germ cell-associated genes, including dnd, ddx4, piwil, gfra1b, sox2, myca, nanog, ly75, and dazl, as determined by semiquantitative assays, and almost all cells were found to express the germ cell genes ddx4 and gfra1b in a fluorescence in situ hybridization assay. In vitro, induction experiments demonstrated the TrSSCs possessed the ability to differentiate into other types of cells. Our research has enriched the fish spermatogonial stem cell resource bank, which will provide an efficient research model for sex determination and sex control breeding in fish, establishing a foundation for subsequent breeding research.

24

Goswami, Mukunda, Yashwanth Belathur Shambhugowda, Arjunan Sathiyanarayanan, Nevil Pinto, Alexandrea Duscher, Reza Ovissipour, Wazir Singh Lakra, and Ravishankar Chandragiri Nagarajarao. "Cellular Aquaculture: Prospects and Challenges." Micromachines 13, no. 6 (May 26, 2022): 828. http://dx.doi.org/10.3390/mi13060828.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Aquaculture plays an important role as one of the fastest-growing food-producing sectors in global food and nutritional security. Demand for animal protein in the form of fish has been increasing tremendously. Aquaculture faces many challenges to produce quality fish for the burgeoning world population. Cellular aquaculture can provide an alternative, climate-resilient food production system to produce quality fish. Potential applications of fish muscle cell lines in cellular aquaculture have raised the importance of developing and characterizing these cell lines. In vitro models, such as the mouse C2C12 cell line, have been extremely useful for expanding knowledge about molecular mechanisms of muscle growth and differentiation in mammals. Such studies are in an infancy stage in teleost due to the unavailability of equivalent permanent muscle cell lines, except a few fish muscle cell lines that have not yet been used for cellular aquaculture. The Prospect of cell-based aquaculture relies on the development of appropriate muscle cells, optimization of cell conditions, and mass production of cells in bioreactors. Hence, it is required to develop and characterize fish muscle cell lines along with their cryopreservation in cell line repositories and production of ideal mass cells in suitably designed bioreactors to overcome current cellular aquaculture challenges.

25

Castro, Rosario, Samuel A. M. Martin, Jun Zou та Christopher J. Secombes. "Establishment of an IFN-γ specific reporter cell line in fish". Fish & Shellfish Immunology 28, № 2 (лютий 2010): 312–19. http://dx.doi.org/10.1016/j.fsi.2009.11.010.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

26

MacLeod, Roderick A. F., Stefan Nagel, Maren Kaufmann, Karin Greulich-Bode, and Hans G. Drexler. "Multicolor-FISH analysis of a natural killer cell line (NK-92)." Leukemia Research 26, no. 11 (November 2002): 1027–33. http://dx.doi.org/10.1016/s0145-2126(02)00055-3.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Brocal, I., A. Falco, V. Mas, A. Rocha, L. Perez, J. M. Coll, and A. Estepa. "Stable expression of bioactive recombinant pleurocidin in a fish cell line." Applied Microbiology and Biotechnology 72, no. 6 (April 25, 2006): 1217–28. http://dx.doi.org/10.1007/s00253-006-0393-7.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Iwamoto, T., T. Nakai, K. Mori, M. Arimoto, and I. Furusawa. "Cloning of the fish cell line SSN-1 for piscine nodaviruses." Diseases of Aquatic Organisms 43 (2000): 81–89. http://dx.doi.org/10.3354/dao043081.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Oh, Y. B., J. S. Lee, and E. H. Park. "Fish Cell Line As an Test System for Analyzing Chromosome Aberrations." Bulletin of Environmental Contamination and Toxicology 67, no. 1 (July 2001): 0006–11. http://dx.doi.org/10.1007/s00128-001-0084-0.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

30

ISA, K., and A. SHIMA. "Transfection and stable expression of a dominant selective marker Ecogpt in a cultured cell line of the fish, Carassius auratus." Journal of Cell Science 88, no. 2 (September 1, 1987): 219–24. http://dx.doi.org/10.1242/jcs.88.2.219.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The synthetic plasmid, pSV2-gpf, was transfected into the cultured fish cells (RBCF-1 line) using polycation and dimethyl sulphoxide. The maximum transfection frequency estimated by colony number in the selection medium was 585 transfectants/5x105 treated cells per 50 ng plasmid DNA. The isolated transfectants expressed the xanthine guanine phosphoribosyltransferase (XGPRT) activity encoded by the plasmid DNA associated with the promoter of simian virus 40 (SV40). The resistance to mycophenolic acid and the XGPRT activity of every transfectant examined were stable, indicating the possibility that pSV2-gpt was integrated into the genomic DNA of the host fish cells. Our results, in addition to those already reported for mammalian and avian cell lines, indicate that the promoter in the early region of SV40 can function in cultured fish cells. This success in obtaining cultured fish cells with a dominant selective marker will provide a useful clue for somatic cell genetic studies of fish in the future.

31

Rivers, Nicola, Jonathan Daly, and Peter Temple-Smith. "New directions in assisted breeding techniques for fish conservation." Reproduction, Fertility and Development 32, no. 9 (2020): 807. http://dx.doi.org/10.1071/rd19457.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Fish populations continue to decline globally, signalling the need for new initiatives to conserve endangered species. Over the past two decades, with advances in our understanding of fish germ line biology, new exsitu management strategies for fish genetics and reproduction have focused on the use of germ line cells. The development of germ cell transplantation techniques for the purposes of propagating fish species, most commonly farmed species such as salmonids, has been gaining interest among conservation scientists as a means of regenerating endangered species. Previously, exsitu conservation methods in fish have been restricted to the cryopreservation of gametes or maintaining captive breeding colonies, both of which face significant challenges that have restricted their widespread implementation. However, advances in germ cell transplantation techniques have made its application in endangered species tangible. Using this approach, it is possible to preserve the genetics of fish species at any stage in their reproductive cycle regardless of sexual maturity or the limitations of brief annual spawning periods. Combining cryopreservation and germ cell transplantation will greatly expand our ability to preserve functional genetic samples from threatened species, to secure fish biodiversity and to produce new individuals to enhance or restore native populations.

32

Qiu, Huiying, Yongquan Xue, Jinlan Pan, Yafang Wu, and Yong Wang. "The Establishment and Characterization of a Human Acute Myelocytic Leukemia Cell Line, SH-2 Carrying t(16;17))(q24;q12) Translocation." Blood 110, no. 11 (November 16, 2007): 4128. http://dx.doi.org/10.1182/blood.v110.11.4128.4128.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Abstract We report the establishment of a novel cell line from a patient with acute myelocytic leukemia. The initial growth of this cell line was dependent on the presence of the stromal cell and cytokine interleukine 3. Subsequently, a stroma and cytokine-independent cell line was established, named SH-2. The cell line has proliferated continuously in vitro for more than 24 months. The SH-2 cell line showed identical chromosomal alterations to that of the initial bone marrow aspiration specimen, demonstrated a t(16;17))(q24;q12) translocation accompanied by deletions of y and 17 and triple 19.Chromosome painting FISH and Multi-FISH conformed the karyotype.SH-2 cells expressed myeloid antigens CD13, CD33 and MPO, SH-2 co-expressed NK markers CD16 and CD56. The loss of one p53 allele were proven by chromosome painting, FISH. A point mutation of CAG to CAT at codon 576 of extron 5 in another p53 allele was found by direct sequencing of DNA. Neither Epstein-Barr virus nor mycoplasma was not detected in SH-2 cells. DNA fingerprinting confirmed the authenticity of the cell line. Cytokines of GM-CSF,IL-3,IL-6,SCF,IL-4 and IL-5 can improve the proliferation of SH-2. SH-2 has colony formality and tumorigenic capacity in nude mice. The breakpoints of chromosomes 16 and 17 were localized RP11-356C4(16q24.3)and between BAC clone RP1-161P9 and RP11-47L3 corresponding to 17q12. The establishment of an myelocytic leukemia cell line with t(16;17)(q24;q12) could be valuable for the study of leukemogenesis and for the research of cloning the new gene involved in the t(16;17)(q24;q12) translocation.

33

Stuart, G. W., J. V. McMurray, and M. Westerfield. "Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos." Development 103, no. 2 (June 1, 1988): 403–12. http://dx.doi.org/10.1242/dev.103.2.403.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

To generate stable lines of transgenic fish, early zebrafish embryos were injected with high concentrations of a linear bacterial plasmid. After injection, the foreign DNA was converted into a high molecular weight form and then amplified approximately tenfold during the initial rapid cleavages characteristic of the early embryo prior to gastrulation. While most of this DNA was subsequently degraded during gastrulation, some of the foreign sequences survived the gastrula stage and could be found in most of the injected fish at 3 weeks of age. Only about 5% of fish analysed 4 months after the injection retained foreign DNA in their fins, usually at less than one copy per cell. One of these fish was also found to contain about 100 copies per cell of foreign DNA in a fraction of its germ cells. Approximately 20% of the F1 offspring from this germ-line-positive parent inherited the foreign DNA, whereas 50% of F2 progeny obtained from an identified F1 individual inherited these sequences. The 50% transmission rate in F2 progeny was as expected for a single, heterozygous genomic insert. These observations indicate that injected DNA can be integrated into the fish genome, that the resulting transgenic fish are mosaic and that some of these mosaic individuals give rise to stable lines of transgenic fish.

34

Han, Dan Hee, and Seung Pyo Gong. "Establishment and Characterization of a Skeletal Muscle-Derived Myogenic Cell Line from Black Sea Bream (Acanthopagrus schlegelii)." Journal of Marine Science and Engineering 12, no. 2 (January 30, 2024): 249. http://dx.doi.org/10.3390/jmse12020249.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Establishing muscle lineage cell lines from fish will provide a great opportunity to study muscle development, which can eventually contribute to the improvement of the fish quality in the aquaculture industry. However, there has been a lack of the development of proper fish muscle lineage cell lines so far. Here, we report the establishment of a skeletal muscle-derived myogenic cell line from black sea bream (Acanthopagrus schlegelii). For this, we first attempted to find the optimal conditions for the primary explant culture of A. schlegelii muscle tissues and then established muscle-derived cell lines. After that, cell lines were characterized for their muscle-specific gene expression, growth, and myogenic differentiation. We found that the primary explant culture was effective when the tissue fragments were cultured in Dulbecco’s Modified Eagle Medium supplemented with fetal bovine serum and antibiotics on gelatin-coated dishes. Additionally, we confirmed that the addition of basic fibroblast growth factor was necessary to establish the cell lines. One of three cell lines established was capable of long-term culture, expressed three major myogenic regulatory genes including Pax7, MyoD, and Myog, and differentiated to myotubes in the condition using low concentration of horse serum, demonstrating that this cell line was a skeletal muscle-derived myogenic cell line.

35

BiBi, Tayyaba, and Taj Muhammad Khan. "Assessment of engineered nanosilver as an alternative nano-antibiotic in marine water pollution using biomarker of fish cell line." Toxicology Research and Application 5 (January 1, 2021): 239784732199828. http://dx.doi.org/10.1177/2397847321998282.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

A large volume of antibiotics is used in fish farms to treat diseases because the farmed fish are fully affected by diseases and parasites in the aquaculture and particularly in the ocean environment where disease pathogens multiply quickly. The frequent use of these antibiotics in aquaculture has resulted in animal; stress, infection, and their dissemination in the form of antibiotic resistant genes to other bacteria including human and animal pathogens. The problems arising with antibiotics can be overcome by using silver nanoparticles (AgNPs) due to their physiochemical properties and low toxicity. So AgNPs could be combined with antibiotics to induce infections in fish cell lines and to protect dissemination of antibiotics in the form of antibiotics resistant genes. We expose AgNPs on fish cell lines as a new nano-antibacterial agent to investigate and obtain findings in terms of the cell viability and toxicity. The experimental data is analyzed to examine the antibacterial effects of nanosilver as a replacement agent and discuss the complex scenario, drawbacks, techniques, methods, main mechanisms, and procedures to perform antibacterial tests of exposed AgNPs. There would be an attempt to deal with the AgNPs antibacterial therapies for the fish cell lines.

36

Morin, Guillaume, Karine Pinel, Karine Dias, Iban Seiliez, and Florian Beaumatin. "RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition." Cells 9, no. 8 (July 22, 2020): 1754. http://dx.doi.org/10.3390/cells9081754.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Nowadays, aquaculture provides more than 50% of fish consumed worldwide but faces new issues that challenge its sustainability. One of them relies on the replacement of fish meal (FM) in aquaculture feeds by other protein sources without deeply affecting the whole organism’s homeostasis. Multiple strategies have already been tested using in vivo approaches, but they hardly managed to cope with the multifactorial problems related to the complexities of fish biology together with new feed formulations. In this context, rainbow trout (RT) is particularly concerned by these problems, since, as a carnivorous fish, dietary proteins provide the amino acids required to supply most of its energetic metabolism. Surprisingly, we noticed that in vitro approaches considering RT cell lines as models to study RT amino acid metabolism were never previously used. Therefore, we decided to investigate if, and how, three major pathways described, in other species, to be regulated by amino acid and to control cellular homeostasis were functional in a RT cell line called RTH-149—namely, the mechanistic Target Of Rapamycin (mTOR), autophagy and the general control nonderepressible 2 (GCN2) pathways. Our results not only demonstrated that these three pathways were functional in RTH-149 cells, but they also highlighted some RT specificities with respect to the time response, amino acid dependencies and the activation levels of their downstream targets. Altogether, this article demonstrated, for the first time, that RT cell lines could represent an interesting alternative of in vivo experimentations for the study of fish nutrition-related questions.

37

Struski, Stéphanie, Martine Doco‐Fenzy, Michael Koehler, Ilse Chudoba, Francis Levy, Linda Masson, Nicole Michel, et al. "Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity." Analytical Cellular Pathology 25, no. 3 (2003): 115–22. http://dx.doi.org/10.1155/2003/151042.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex‐FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug‐resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). Colour figure can be viewed onhttp://www.esacp.org/acp/2003/25‐3/struski.htm.

38

Parrow, MW, JAM Burkholder, NJ Deamer, and JS Ramsdell. "Contaminant-free cultivation of Pfiesteria shumwayae (Dinophyceae) on a fish cell line." Aquatic Microbial Ecology 39 (2005): 97–105. http://dx.doi.org/10.3354/ame039097.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Trento, Marcus Vinicius Cardoso, Pedro Henrique Souza César, Silvana Marcussi, and Lucy E. J. Lee. "Genotoxic action of naphthenic acids on the fish macrophage cell line, RTS11." International Journal of Environment and Pollution 63, no. 1/2 (2018): 117. http://dx.doi.org/10.1504/ijep.2018.093045.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Trento, Marcus Vinicius Cardoso, Pedro Henrique Souza César, Silvana Marcussi, and Lucy E. J. Lee. "Genotoxic action of naphthenic acids on the fish macrophage cell line, RTS11." International Journal of Environment and Pollution 63, no. 1/2 (2018): 117. http://dx.doi.org/10.1504/ijep.2018.10014156.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Lammel, Tobias, Maria-Luisa Fernández-Cruz, Mona Connolly, Barrado Ana Isabel Conde Estefania, Sylvain Derick, Yolanda Pérez, Marta Fernández, Christophe Furger, and José Maria Navas. "Toxicity of zinc oxide nanoparticles towards a fish and mammalian cell line." Toxicology Letters 211 (June 2012): S202. http://dx.doi.org/10.1016/j.toxlet.2012.03.726.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Puerto, María, Silvia Pichardo, Ángeles Jos, and Ana M. Cameán. "Oxidative stress induced by microcystin–LR on PLHC-1 fish cell line." Toxicology in Vitro 23, no. 8 (December 2009): 1445–49. http://dx.doi.org/10.1016/j.tiv.2009.08.011.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Minghetti, Matteo, Michael J. Leaver, John B. Taggart, Elisa Casadei, Meirav Auslander, Moshe Tom, and Stephen G. George. "Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1." Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 154, no. 2 (August 2011): 93–99. http://dx.doi.org/10.1016/j.cbpc.2011.03.010.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Iwamoto, T., K. Mori, M. Arimoto, and T. Nakai. "High permissivity of the fish cell line SSN-1 for piscine nodaviruses." Diseases of Aquatic Organisms 39 (1999): 37–47. http://dx.doi.org/10.3354/dao039037.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Riva, M. C., P. Alañón, and A. Castaño. "Cytotoxicity of Leather Processing Effluents on the RTG-2 Fish Cell Line." Bulletin of Environmental Contamination and Toxicology 75, no. 1 (July 2005): 34–41. http://dx.doi.org/10.1007/s00128-005-0715-y.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Ferrero, M., A. Castaño, A. Gonzalez, F. Sanz, and C. Becerril. "Characterization of RTG-2 Fish Cell Line by Random Amplified Polymorphic DNA." Ecotoxicology and Environmental Safety 40, no. 1-2 (May 1998): 56–64. http://dx.doi.org/10.1006/eesa.1998.1642.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Chen, Xiao, Yuting Kan, Ying Zhong, Muhammad Jawad, Wenbo Wei, Kaiyan Gu, Lang Gui, and Mingyou Li. "Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro." Biology 11, no. 7 (July 18, 2022): 1069. http://dx.doi.org/10.3390/biology11071069.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Opsariichthys bidens belongs to the family Cyprinidae and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of O. bidens have been drastically reduced due to overfishing and the destruction of the water environment. The in vitro culture and long-term preservation of germ stem cells are the key technologies to keep genetic resources from degeneration. However, except for the establishment of the first long-term cultured medaka spermatogonia cell line (SSC) capable of producing sperm in vitro in 2004, no other long-term cultured SSC line has been found in other fish species. In this study, we successfully established another long-term-cultured spermatogonial stem cell line from Opsariichthys bidens (ObSSC). After more than 2 years of culture, ObSSC had a diploid karyotype and stable growth, with the typical gene expression patterns of SSC. Under in vitro culture, ObSSC could be induced to differentiate into sperm and other different types of somatic cells. In vivo, ObSSC could differentiate into different cells of three germ layers upon being transplanted into zebrafish embryos. Our research helps to explore the potential and regulation mechanism of fish SSC differentiation and spermatogenesis in vitro, provides a new way for solving the problem of fish genetic resource degradation and lays a foundation for further research on fish germ cell transplantation.

48

Crooker, A. R., E. W. Devlin, D. E. Johnson, and N. K. Mottet. "Comparative ultrastructural pathology of methyl mercury-induced lesions in a teleostean and a mammalian cell line." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 362–63. http://dx.doi.org/10.1017/s0424820100143432.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Toxic organic mercury compounds, especially methyl mercury (MeHg), have been responsible for severe environmental contamination. Industrial effluents of inorganic or metallic mercury are methylated by aquatic organisms leading to transfer and bioconcentration of MeHg up the food chain to fish and man. In vivo mammalian and teleostean (bony fish) models have provided useful data on mercury toxicity; cultures of animal cells have been used to investigate the toxic actions of heavy metals such as mercury. The present study was designed to determine if RTG-2 cells, a fibroblast-like line derived from rainbow trout (Salmo gairdneri) gonads, would provide a highly controlled system in which to study the mercury-cell interaction. A mammalian cell line derived from Chinese hamster ovary (CHO)2 and known to be sensitive to MeHg3 was chosen as a positive control. The specific objective was to determine the effect of MeHg on RTG-2 and CHO cell viability and ultrastructure.

49

Bernal-Algaba, Elena, Marta Pulgarín-Alfaro, and María Luisa Fernández-Cruz. "Cytotoxicity of Mycotoxins Frequently Present in Aquafeeds to the Fish Cell Line RTGill-W1." Toxins 13, no. 8 (August 20, 2021): 581. http://dx.doi.org/10.3390/toxins13080581.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

In the last decades, the aquaculture industry has introduced plant-based ingredients as a source of protein in aquafeeds. This has led to mycotoxin contaminations, representing an ecological, health and economic problem. The aim of this study was to determine in the RTgill-W1 fish cell line the toxicity of fifteen mycotoxins of common occurrence in aquafeeds. To identify the most sensitive endpoint of toxicity, the triple assay was used. It consisted of three assays: alamarBlue, Neutral Red Uptake and CFDA-AM, which revealed the mitochondrial activity, the lysosomal integrity and the plasma membrane integrity, respectively. Most of the assayed mycotoxins were toxic predominantly at lysosomal level (enniatins, beauvericin, zearalenone, ochratoxin A, deoxynivalenol (DON) and its acetylated metabolites 15-O-acetyl-DON and 3-acetyl-DON). Aflatoxins B1 and B2 exerted the greatest effects at mitochondrial level, while fumonisins B1 and B2 and nivalenol were not toxic up to 100 µg/mL. In general, low toxicity was observed at plasma membrane level. The vast majority of the mycotoxins assayed exerted a pronounced acute effect in the fish RTgill-W1 cell line, emphasizing the need for further studies to ascertain the impact of mycotoxin contamination of fish feeds in the aquaculture industry and to establish safe limits in aquafeeds.

50

Espinosa-Ruiz, Cristóbal, Javier Mayor-Lafuente, and M. Ángeles Esteban. "Mitochondrial Metabolism Characterization of Four Different Fish Cell Lines." Fishes 7, no. 6 (November 28, 2022): 354. http://dx.doi.org/10.3390/fishes7060354.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Mitochondria are a crucial cellular organelle that organizes a wide range of biological processes, from energy production and calcium homeostasis to cell proliferation, differentiation, and death as well as inflammation. Mitochondria also support immune cell phenotypes and function. The aim of this communication is to evaluate the mitochondrial status and plasticity of four fish cell lines: SAF-1 (derived from gilthead sea bream skin), DLB-1 (derived from European sea bass brain), FuB-1 (established from mummichog brain), and PLHC-1 (a topminnow hepatocellular carcinoma). A mitochondrial stress assay was performed to compare the bioenergetic capabilities of these four fish cell lines as well as the importance of choosing the correct cell line when performing different in vitro studies.

Статті в журналах з теми "Fish cell line"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями