Дисертації з теми "Fetal lamb"
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Vugt, Johannes Marinus Gerardus van. "Fetal artery Doppler velocimetry a study in the human fetus and the fetal lamb /." Maastricht ; Rijksuniversiteit Limburg ; Maastricht : University Library, Maastricht University [Host], 1988. http://arno.unimaas.nl/show.cgi?fid=5436.
Повний текст джерелаShariff, Mohammed Azamatulla. "Kinetics of selenium metabolism in the ewe and fetal lamb." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27528.
Повний текст джерелаLand and Food Systems, Faculty of
Graduate
Van, der Weyde Marlene P. "The effects of the beta-adrenergic agonist, ritodrine, in the fetal lamb." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30417.
Повний текст джерелаMedicine, Faculty of
Obstetrics and Gynaecology, Department of
Graduate
Knight, Kathleen Elizabeth. "Hindlimb blood flow and oxygen consumption in the late gestation fetal lamb." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0031/NQ38915.pdf.
Повний текст джерелаSmith, Gordon C. S. "The expression of prostanoid receptor genes in uterine and fetal tissues : studies in the maternal and fetal baboon and the fetal and neonatal lamb." Thesis, University of Glasgow, 2000. http://theses.gla.ac.uk/40962/.
Повний текст джерелаMorrison, Janna L. "Physiologic responses of the fetal lamb to eight day fluoxetine exposure during late gestation." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ61148.pdf.
Повний текст джерелаKleemann, D. O. "A study of factors affecting embryonic, fetal and lamb survival in high fecundity merino ewes." Title page, table of contents and abstract only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phk638.pdf.
Повний текст джерелаBurry, Andrea F. "The inter-relation of TGF-Beta, LC3 and apolipoprotein D in the fetal lamb ductus arteriosus." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0011/MQ40768.pdf.
Повний текст джерелаBratu, Ioana. "Long growth, structural remodeling, surfactant levels, and lung function after reversible fetal lamb tracheal occlusion in congenital diaphragmatic hernia." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33383.
Повний текст джерелаButter, Andreana. "Lung growth and lung function after a) fetal lamb tracheal occlusion and exogenous surfactant at birth in congenital diaphragmatic hernia and b) selective perfluorocarbon distention in healthy newborn piglets." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33073.
Повний текст джерелаPostnatal intervention involved perfluorocarbon (PFC) liquid distention of the right upper lobe in healthy newborn piglets. Postnatal lung growth, as measured indirectly by rates of DNA synthesis, was not accelerated after PFC distention.
Haan, Harmen Hendrik de. "Fetal asphyxia a study in preterm lambs /." Maastricht : Maastricht : Rijksuniversiteit Limburg ; University Library, Maastricht University [Host], 1994. http://arno.unimaas.nl/show.cgi?fid=6746.
Повний текст джерелаDunn, Stacey Leanne. "Fetal growth restriction : effects on the adrenal cortex in postnatal lambs /." Title page and abstract only, 2004. http://web4.library.adelaide.edu.au/theses/09SB/09sbd9231.pdf.
Повний текст джерелаRocha, Bruno Garcia. "Estabelecimento de metodologias de análise do DNA livre plasmático para o diagnóstico pré-natal não invasivo : sexagem fetal." Universidade Federal de São Carlos, 2011. https://repositorio.ufscar.br/handle/ufscar/6993.
Повний текст джерелаIn this paper we proposed and analyzed methodologies using the technology of Cell-Free Fetal Nucleic Acid-Free (cffDNA = Cell Free Fetal DNA) in noninvasive prenatal diagnosis (NIPD). Due to the modern technologies employed and their repercussion among the involved families, we sought to discuss some ethical, social and legal implications. Contrary to the popular belief that the placenta forms an impermeable barrier between mother and child, there is bidirectional traffic between the fetus and mother during pregnancy. Several studies have shown that not only intact cells but also fetal cell-free fetal nucleic acids (cffNA, ie, DNA and RNA) cross the placenta and travels in the mother's bloodstream. Four different applications of analysis technology were identified: cffDNA: a) Prenatal Sex Determination, used in pregnancies under the risk of sexual transmitted diseases and performed through the detection of the Y chromosome b) The diagnosis of certain diseases of a single gene through the detection of paternally inherited mutation c) Fetal Aneuploidy, such as Down syndrome, where chromosomal abnormalities may occur d) identifying the type of fetal blood in pregnancies under the risk of incompatibility, especially RhD. To carry out this work it was used the pre-natal determination of sex in 53 pregnant women at different gestational periods. For that it was proposed and tested methods of DNA extraction, amplification of DNA obtained by PCR (Polymerase Chain Reaction) and a new methodology called LAMP (Loop Mediated Isothermal Amplification) and the analysis of final results. Furthermore, the efficiency of LAMP and PCR was compared by amplifying different segments of the Y chromosome: DSY14 and TSPY. In three cases the samples were discarded because there was no fetal sex confirmation after molecular tests due to loss of contact with pregnant women. In two other cases the results pointed to male fetuses whilst the ultrasound confirmed these fetuses as females due to contamination. Finally it was obtained 28 male samples (58.33%) with amplification of the sequences of the Y chromosome and 20 female samples (41.67%) that did not amplify the sequence of the Y chromosome but only for the control. These results showed that the amplification lamp is more efficient than PCR, in the analysis of DSY14, the limit of detection is 10 pg and 0.1 pg for LAMP and PCR respectively. It was concluded that the amplification using the LAMP method is faster (60 min) and has a high sensitivity and specificity and does not require sophisticated equipment for reaction if compared to the PCR method. These characteristics make this methodology feaseable in laboratories with limited resources.
Neste trabalho propusemos e analisamos metodologias empregadas no uso da tecnologia dos Ácidos Nucléicos Livres de Células Fetais (cffDNA = Cell Free Fetal DNA) no diagnóstico pré-natal não invasivo (DPIN = Non-invasive Prenatal Diagnosis). Dada a modernidade das tecnologias empregadas e a sua repercussão nas famílias envolvidas, procuramos discutir algumas implicações éticas, sociais e legais. Ao contrário da crença popular de que a placenta constitui uma barreira impermeável entre mãe e filho, há tráfego bidirecional entre o feto e a mãe durante a gravidez. Vários estudos têm demonstrado que tanto células fetais intactas e ácidos nucléicos livres de células fetais (cffNA, ou seja, DNA e RNA) atravessam a placenta e circulam na corrente sanguínea materna. Identificamos quatro diferentes aplicações da tecnologia de análise do cffDNA: a) Determinação pré-natal do sexo, utilizada em gestações com risco de uma doença ligada ao sexo e realizada através da detecção do cromossomo masculino Y; b) O diagnóstico de certas doenças de gene único, normalmente através da detecção de mutação herdada paternalmente; c) Aneuploidia fetal, tal como a Síndrome de Down, onde há alterações cromossômicas; d) Detecção do tipo de sangue fetal em gestações com risco de incompatibilidade, sobretudo RhD. Para realizar este trabalho utilizamos a determinação pré-natal do sexo em 53 gestantes em diferentes períodos gestacionais. Para tal, testamos e propusemos metodologias de extração do DNA; amplificação do DNA extraído através da técnica da PCR (Polymerase Chain Reaction) e de uma nova metodologia denominada LAMP (Loop Mediated Isothermal Amplification); e a análise final dos resultados. Além disso, comparamos a eficiência da PCR e LAMP amplificando diferentes segmentos do cromossomo Y: DSY14 e TSPY. Em três casos as amostras foram descartadas porque não houve a confirmação do sexo fetal após os testes moleculares devido à perda de contato com as gestantes. Em outros dois casos os resultados apontaram para fetos masculinos, entretanto, no ultra-som eles foram confirmados como femininos, provavelmente devido a uma contaminção. No final obtivemos 28 amostras (58,33%) masculinas, com a amplificação das sequências do cromossomo Y e 20 amostras (41,67%) femininas que não amplificaram a sequência do cromossomo Y e apenas para o controle. Nossos resultados demonstraram que a amplificação por LAMP é mais eficiente que a PCR na análise de DSY14, sendo que o limite de detecção é de 10 pg e 0,1 pg, para a PCR e o LAMP, respectivamente. Concluímos que a amplificação utilizando a metodologia de LAMP é mais rápida (60 minutos) e apresenta uma alta especificidade, sensibilidade e não requer equipamentos sofisticados para reação, comparada com a técnica da PCR. Tais características viabilizam esta metodologia em laboratórios com poucos recursos.
Longo, Ana Luisa Silva. "Cortisol fecal em ovinos: curva de excreção e estabilidade." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/74/74135/tde-11052016-092231/.
Повний текст джерелаThis present study was divided into two experiments, aiming to determine the excretion curve of faecal cortisol and its stability over different periods of time and temperature between harvest and analyzes, correlating the fecal cortisol levels with peak blood cortisol. The project was developed in Biometeorology and Ethology Lab, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga - SP. In the first experiment, six crossbred (Dorper x Santa Inês) females sheep had their total feces collected during 24 hours after the application of adrenocorticotropic hormone (ACTH), beyond the blood samples taken before the application of ACTH, and one, two and five hours after application; in which was attributed reactivity scores to each animal. Soon after the analysis was started the second experiment, in which nine crossbred lambs (Dorper x Santa Inês) underwent a situation of thermal stress from 11 to 15 pm, and their feces were collected at 23 hours the same day. After harveting, the feces were pooled and homogenized in three different groups, where aliquots were withdrawn relating to the treatments proposed: three temperatures (15, 25 and 35°C) and four times (1, 3, 6 and 12 hours). The excretion curve data were analyzed by ANOVA, as well as the correlation between blood cortisol levels, faecal and reactivity. For stability analysis were used multifactor ANOVA with two factors (temperature and time range). To evaluate the behavioral variables was performed the transformation of the data range for \"arc sine percentage root\", proceeding to the analysis of variance. The statistical model included effects of day (1, 2 and 3) with individual analysis by animal. The blood cortisol parameters, respiratory rate and rectal temperature were analyzed by t test Pearson correlation. All comparisons of means were performed by F and t test (pdiff). The reactivity during harvest did not exert significant effect on blood cortisol levels, which showed higher averages 60 minutes after the application of ACTH, and after 300 minutes, the sheep showed cortisol levels considered normal to them, without stress. On the other hand, the peak of cortisol in the feces was observed approximately 10-12 hours after the peak of cortisol in the blood, not being observed significant decreases that indicate the return to the basal levels during the 24 hour period (P>0, 05). Were not observed no significant differences between the time and temperature in which the faecal samples were subjected (P>0.05), verifying a tendency on the maintenance of the concentration of faecal cortisol in sheep during the 12 hour period after harvest.
Huisseling, Johannes Caspar Maria van. "Umbilical artery blood flow velocity waveforms and placental vascular resistance a study in fetal lambs /." Maastricht : Maastricht : Datawyse ; University Library, Maastricht University [Host], 1990. http://arno.unimaas.nl/show.cgi?fid=5601.
Повний текст джерелаJunior, João Carlos Morini. "Glicogênio placentário e fetal originados de ovinos gestantes submetidos ou não a tosquia." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-14022008-163944/.
Повний текст джерелаIn order to clarify the association of weight increase of the fetus in the act of shorn, and remembered that this fact decreases the rate of mortality at birth we tested twenty Merino australian sheep. Animals were divided into 2 groups with 10 members each: shorn (OT) to 70 days of gestational period and a second kept as control (ONT). The placenta and fetus were removed by a surgical procedure of caesarean, according conventional technique. As characterization of the placenta quantification of the placentome numbers and their measures. Samples of placenta were fixed and routinely processed for the light microscopy, with inclusion in paraffin. The quantification of glycogen was obtained with the measurement of 5 fields in each slides produced sequentially. The tests were conducted by comparative effect of a difference in color between the slides and the area covered by the histochemistry PAS reaction For statistical analysis was used the T test (student) and comparison between normal averages. The macroscopic results indicated that the treatment of shorn not significantly increased the fetal and placental weights, and so little measures regarding placentomes. The microscopic analysis, shows that the average between the areas of glycogen quantified in placentome were not different (P <0.05). Already in the liver at the portal triad region and in the rectus femoris muscle were found a significant increase of glycogen deposits (P <0.05). With this results we can conclude that the shorn done at 70 days of gestational period influence on the accumulation of glycogen in the liver and muscle of the fetuses and thus increase the weight of them at birth.
Leisinger, Chelsey Audra. "Factors affecting in vitro maturation of alpaca (Lama paco) oocytes." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1373989536.
Повний текст джерелаRosa, Fabiane Quevedo da. "Consumo e disgestibilidade de pastagem nativa do bioma pampa e inclusões de azevém." Universidade Federal do Pampa, 2016. http://dspace.unipampa.edu.br:8080/xmlui/handle/riu/541.
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Com o objetivo de avaliar a proteína bruta fecal como indicador de consumo e digestibilidade de bovinos alimentados com pastagem nativa situada no Bioma Pampa e inclusões de azevém e avaliar a qualidade nutricional dessas pastagens foram realizados dois experimentos em gaiolas de metabolismo. No experimento I, os animais recebiam diferentes níveis de oferta de pastagem nativa onde os tratamentos consistiam em níveis de1,5 e 2,25% do peso vivo de matéria seca e um nível ad libitum, com pelo menos 20% de sobras diárias onde foram realizados seis períodos experimentais, O experimento II consistia em diferentes níveis de inclusão de azevém na dieta de bovinos alimentados com campo nativo, os tratamentos eram 33%, 66% e 100% de azevém em substituição ao campo nativo, em dois períodos experimentias. Os experimentos foram realizados em delineamento inteiramente casualizado com 3 tratamentos e 2 repetições por tratamento em cada período. Os períodos experimentais consistiram de 10 dias de adaptação e 5 dias de coletas, durante o período de coletas foram amostradas as sobras do cocho, a forragem ofertada e ainda a produção total de fezes a cada 24 horas. Realizou-se as medidas de produção fecal, concentração de componentes fecais, consumo de nutrientes e digestibilidade dos componentes da dieta. No capítulo I foram traçadas relações entre digestibilidade e a concentração da proteína bruta fecal (PBf), sendo testados dois modelos não lineares, o exponencial e o hiperbólico gerando as equações de digestibilidade da matéria orgânica(DMO) = 0,709-9,506* exp(-0,041*PBf) com R2 0,61 e DMO = 0,942-38,619/PBf (R2 0,62), respectivamente. A relação de consumo de matéria orgânica (CMO) com a quantidade de PBf, possui comportamento linear, foi então realizada a análise de stepwise para saber quais outras variávies poderiam explicar melhor o modelo junto com a PBf gerando uma equação CMO = -6724,30 + 39*PBf + 2,55*FDNf + 11591,44*DMO com R2 0,95. Determinou-se a relação da proteína bruta (PB) da dieta (g/kg MO) x PBfecal (g/kg MO), PB dieta = 1,346x - 47,63 R² = 0,931 (modelo linear). No capítulo II, foram testados dois modelos para todas as relações traçadas no experimento II, um modelo linear e um modelo quadrático, as relações foram consumo de matéria orgânica (CMO (g/UTM)) x digestibilidade da fibra em detergente neutro (DFDN), DMO x CMO (g/UTM), DMO x CMO (g/dia), consumo de folhas (g/UTM) x DMO e CMO (g/UTM) x relação proteína/energia. As relações que não foram estatisticamente significativas para nenhum modelo foram CMO (g/UTM) x relação proteína/energia e consumo de folhas (g/UTM) x DMO. Após essas relações, verificou-se que a PBf tem um bom potencial para a estimativa de consumo e a digestibilidade por bovinos alimentados com pastagens nativas ou associadas a forragem cultivada como o azevém anual, mas estes parâmetros também são afetados por diferentes variáveis.
In order to evaluate the fecal crude protein as intake and digestibility marker of cattle fed with natural pasture from Pampa Biome and annual ryegrass inclusions and evaluate the nutritional quality of these pastures it was conducted two experiments in metabolic cages. In the first experiment, the animals received different native pasture offer levels where treatments consisted of 1.5 and 2.25% of the live weight of dry matter and ad libitum with at least 20% of daily leftovers which were carried out six experimental periods. The second experiment consisted of annual ryegrass inclusion levels in the diet of cattle fed with natural pasture, the treatments were 33%, 66% and 100% of ryegrass to replace the native pasture in two experimental periods. The experiments were carried out in a completely randomized design with 3 treatments and 2 replicates per treatment in each period. The experimental period consisted of 10 days of adaptation and 5 days of collection during the period of the trough collects the forage offered have been sampled, the leftovers and also the total fecal production in each 24 hours. It was measured the fecal productions, concentration of faecal components, nutrient intake and digestibility of diet components. In the Chapter I were study the relationship between digestibility and fecal concentration of crude protein (CPf), being tested exponential and hyperbolic equations generating the digestibility of organic matter (OMD) = 0.709 - 9.506 * exp (- 0.041 * CPf) with R2 = 0.61 and OMD =0.942 - 38.619 / CPf (R2 0.62), respectively. The ratio of organic matter intake (OMI) with the amount of CPf, has a linear relation, it was then carried out stepwise analysis to find out what other variables could explain better the model along with the CPf generating a equation OMI = -6724.30 + 0,998 * CPf + 2.55 * NDFf + 11591.44 * OMD with R2= 0.95. It was determined the ratio of crude protein (CP) of the diet (g/kg of OM) x CPf (g/kg of OM), CPdiet = 1,346*CPf - 47.63 R² = 0.931 (linear model). In the Chapter II were tested two models for all relationships outlined in experiment II, a linear model and a quadratic model, relations were organic matter intake (OMI) x digestibility of neutral detergent fiber (DNDF), OMD x OMI, leaves intake x OMD and OMI x protein/energy ratio. The relationships that were not statistically significant for either model were OMI x protein/energy and leaves intake x OMD. After these relationships, it was found that the CPf has a good potential to estimate intake and digestibility in cattle fed native pastures or associated with annual ryegrass, but these parameters are also affected by different variables.
Coleman, Danielle Nicole. "The effects of supplementing EPA and DHA during late gestation on ewes and lambs." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1498824557998868.
Повний текст джерелаPaganoni, Beth Louise. "Increasing feed-on-offer to merino ewes during pregnancy and lactation can increase muscle and decrease fat, but does not affect the faecal worm egg count of their progeny." University of Western Australia, 2005. http://theses.library.uwa.edu.au/adt-WU2005.0129.
Повний текст джерелаKnight, Kathleen Elizaabeth. "Hindlimb blood flow and oxygen consumption in the late gestation fetal lamb." Thesis, 1998. http://hdl.handle.net/2429/9896.
Повний текст джерелаWoodall, John Paul. "The ontogeny of interferon-gamma (IFN-[gamma]) responses in the fetal lamb." Phd thesis, 2005. http://hdl.handle.net/1885/148799.
Повний текст джерелаKleemann, David Oswald. "A study of factors affecting embryonic, fetal and lamb survival in high fecundity merino ewes." Thesis, 1994. http://hdl.handle.net/2440/80346.
Повний текст джерелаThesis (Ph.D.) -- Dept. of Obstetrics and Gynaecology; Dept. of Animal Sciences, 1994
Morrison, Janna Leigh. "Physiologic responses of the fetal lamb to eight day fluoxetine exposure during late gestation." Thesis, 2001. http://hdl.handle.net/2429/13104.
Повний текст джерелаKrishna, Rajesh. "Indomethacin : Capillary gas chromatographic-electron capture detection analysis and pharmacokinetic studies in the fetal lamb." Thesis, 1995. http://hdl.handle.net/2429/3557.
Повний текст джерелаSharma, Rajesh. "The effects of uterine environment upon embryonic, fetal, neonatal and post-natal development and glucose metabolism in sheep : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1689.
Повний текст джерелаStevens, Michelle E. A. "Ovine fetal lung development : the effects of thyroidectomy." Phd thesis, 2003. http://hdl.handle.net/1885/148615.
Повний текст джерелаMuhlhausler, Beverly Sara. "Maternal overnutrition and the regulation of energy balance and appetite before and after birth." 2006. http://hdl.handle.net/2440/38006.
Повний текст джерелаThesis (Ph.D.)--School of Molecular and Biomedical Science, 2006.
Alitheen, Noorjahan Mohamed. "An in vitro model of B lymphocyte development in the Peyer's patches of fetal lambs." Phd thesis, 2001. http://hdl.handle.net/1885/144959.
Повний текст джерела