Дисертації з теми "Fats modification"
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BARLETTA, LUCA. "Random enzymatic interesterification of Palm Olein for future industrial applications." Doctoral thesis, Politecnico di Torino, 2014. http://hdl.handle.net/11583/2528299.
Повний текст джерелаSmith, Leanna Marie. "Fast Corn Grading System Verification and Modification." OpenSIUC, 2012. https://opensiuc.lib.siu.edu/theses/795.
Повний текст джерелаRajah, Kanes K. "Fractionation of milk fat." Thesis, University of Reading, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233844.
Повний текст джерелаShi, Chuan. "Protein Modification and Catabolic Fates of Lipid Peroxidation Products." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1481231287497328.
Повний текст джерелаCampbell, Pearl. "Pou5f1 Post-translational Modifications Modulate Gene Expression and Cell Fate." Thèse, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23607.
Повний текст джерелаHudson, Oliver Thomas. "Polymer modification of fast growing woods for improved mechanical response and sustainable architecture." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610241.
Повний текст джерелаPugsley, Gustavo de Revorêdo. "O efeito modificativo do fato jurídico sobre a relação jurídica obrigacional." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/2/2131/tde-12042015-180612/.
Повний текст джерелаCurrent legal theory generally considers the effects of legal facts (those resulting from the correlation between facts and norms) on the creation, modification or termination of legal relationships. While most authors addresses how legal facts create these relationships, the present study is devoted to legal facts tending to modify the legal relationship, particularly in the field of the Law of Obligations (duty to perform). At first, a criterion must be determined: modification of the isolated right or modification of the Obligation? In \"complex\" obligational relationships, there are many mutual rights and duties, such that the extinction of a right can result only in the modification of the relationship fully considered. Long ago, Savigny asserted that a reasonable analysis of a right must be based on a thorough understanding of the legal relationship at issue. There are now renewed reasons to adopt these relational criteria when analyzing legal relationships. The obligational relationship may be seen as a process, which leads to the reinforcement of its identity with the inclusion of a finalistic element. The ideas of modification and identity are linked, because if a \"change\" leads to the loss of identity, the legal relationship may, in fact, have been terminated. Most of the authors on the subject of modifications addresses the \"transfer of obligations\". Some other studies, beside these subjective modifications, addresses objective modifications, usually restricted to those made by agreement. The \"Pontes de Miranda\" classification, may serve as a useful reference when analyzing the issue of modifications. The author points to modifications by agreement, changes by dolus and culpa (fault, tort law and breach of contract), by force majeure or unforeseeable circumstances, impossibility of performance, and, ultimately, by the mora debitoris and mora creditoris/accipiendi (default, delay of performance) as modifications \"without breaking the identity of the legal relationship\".
Christopher, Bridgette A. "The Influence of Myofilament Protein Modification and Myocardial Insulin Resistance on Pathologic Left Ventricular Function." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1296007529.
Повний текст джерелаStoilova, Bilyana. "Structural plasticity and post-translational modifications of C/EBP beta direct distinct myeloid cell fates." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16742.
Повний текст джерелаThe CCAAT enhancer binding protein beta (C/EBPβ) transcription factor regulates differentiation, proliferation, and functionality of many cell types, including various cells of the immune system. A detailed molecular understanding of how C/EBPβ directs alternative cell fates remains largely elusive. Ectopic expression of C/EBPβ has been previously shown to reprogram committed B cell progenitors into inflammatory macrophages. We took advantage of this reprogramming system in order to examine how C/EBPβ regulates (trans)differentiation. To determine which C/EBPβ protein modules are important for reprogramming, C/EBPβ wild type isoforms and mutants were ectopically expressed in primary mouse B cell progenitors. The data showed that the translationally regulated long isoforms LAP* and LAP, but not the N-terminally truncated isoform LIP can reprogram lymphoid cells into myeloid cells. Furthermore, we found that conserved regions 2,3 and 4 in the C/EBPβ protein transactivation domain are necessary and sufficient for B-to-myeloid cell conversion. Interestingly, the reprogrammed myeloid cells were found to represent a heterogeneous mixture of different myeloid cell types. Detailed analyses of the reprogrammed CD11b+ cells revealed that discrete conserved regions in C/EBPβ activated distinct pro- and anti-inflammatory genes and triggered divergent differentiation programs. Moreover, not only structural C/EBPβ mutants, but also post-translational modification (PTM) site mutations led to different reprogramming outcomes. These data suggest that C/EBPβ orchestrates myeloid diversification by integrating PTMs with structural plasticity as signal dependent adaptable modular properties to determine cell fate.
Guecha, Lopez Carlos Alfonso. "La modification du contrat administratif en droit colombien." Thesis, Paris 2, 2015. http://www.theses.fr/2015PA020037/document.
Повний текст джерелаAlthough the modification of the public contract is in principle free, it can only take place under certain limitations. In France, the rules of advertising and competition impose substantive conditions of form and procedure to determine the legality of the modifying instrument. The prohibition of agreeing to a new object, restrictions on altering the economic balance, setting financial thresholds, etc., reveal a rigorous control for any modifications. In Colombia, however, it appears that the freedom of the parties is much broader. A general threshold of fifty percent of the initial price, the possibility of altering the economics of the contract and even the fundamental modification of the object, are part of a legal system that tends to abolish all restrictions to the will of the parties regarding modification. Although it is of the essence of contract modifications to disclose the conditions initially established, a major theme is that of the nature of the change in Colombian law to reveal all the challenges of its implementation
Jarymowycz, Virginia A. "Studies of the influence of structural modifications or binding on the fast time scale dynamics of proteins." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3322532.
Повний текст джерелаTitle from PDF t.p. (viewed May 8, 2009). Source: Dissertation Abstracts International, Volume: 69-07, Section: B, page: 4156. Adviser: Martin J. Stone.
Чобіт, М. Р., В. П. Васильєв та Ю. В. Панченко. "Модифікація поверхні крейди відпрацьованими рослинними жирами". Thesis, Cумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/46388.
Повний текст джерелаAlan, Salih. "Development Of A Fast Analytical Method For Prediction Of Part Dynamics In Machining Stability Analysis." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/2/12611096/index.pdf.
Повний текст джерелаReeves, Valerie Lynn. "A DIET ENRICHED IN STEARIC ACID PROTECTS AGAINST THE PROGRESSION OF TYPE 2 DIABETES IN LEPTIN RECEPTOR DEFICIENT MICE (DB/DB)." UKnowledge, 2012. http://uknowledge.uky.edu/physiology_etds/3.
Повний текст джерелаLou, Ruding. "Modification of semantically enriched FE mesh models : Application to the fast prototyping of alternative solutions in the context of industrial maintenance." Paris, ENSAM, 2010. http://www.theses.fr/2010ENAMA017.
Повний текст джерелаBehaviour analysis is largely performed on the virtual model of the product before its physical manufacturing. Anyhow, the process could be further optimised especially during the product behaviour optimisation phase. This process involves repetition of four main processing steps: CAD design, mesh creation, enrichment of physical semantics and Finite Element Analysis (FEA). The product behaviour analysis is performed on the first design solution as well as on the numerous successive product optimisation loops. Each design solution evaluation necessitates the same time as required for the first product design and it is particularly crucial in the context of maintenance and lifecycle assessment. This thesis proposes a new framework for CAD-less product optimisation through FEA, which reduces the mesh preparation and semantics enrichment activities. More concretely, the idea is to directly operate the firstly created FE mesh, enriched with semantics, to perform the product modifications required to achieve its optimised version. In this thesis, the underlying concepts and the devised components for the development of such CAD-less operator are discussed. A high-level operator specification is proposed according to a modular structure that allows an easy realisation of different mesh modification operators. Finally, four instances of this high-level operator are described: merging, cracking, drilling and filleting. These operators are prototyped and validated on academic and industrial FE mesh models, thus clearly showing the feasibility of our approach
Adam, Mohammed A. "Fate of the transferrin receptor during in vitro maturation of sheep reticulocytes and post-translational modifications of the transferrin receptor." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=70354.
Повний текст джерелаThe transferrin receptor can be fatty acylated and phosphorylated in intact cells and in isolated plasma membranes. The phosphorylation of the receptor is not affected by the binding of transferrin or anti-transferrin receptor antibody. However, $ beta$-phorbol esters can stimulate the phosphorylation of the receptor suggesting that protein kinase C may be responsible for receptor phosphorylation. During reticulocyte maturation, the externalized transferrin receptor is not phosphorylated. Furthermore, the ability of this externalized receptor to undergo phosphorylation by protein kinase C is also lost, suggesting a maturation induced change in the receptor compared to the cell associated receptor. This change may be a signal which determines whether the transferrin receptor is to be segregated for externalization during red cell maturation or recycled for iron delivery.
Torcal, Garcia Guillem 1991. "Methylation of a lineage-instructive transcription factor acts as a cell fate switch." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2022. http://hdl.handle.net/10803/673532.
Повний текст джерелаLos factores de transcripción instructores de linaje orquestan decisiones de identidad celular a través de la regulación de programas de expresión génica. Sin embargo, cómo su función está regulada durante el desarrollo todavía se desconoce. C/EBPα es un factor de transcripción que juega un papel clave en varias decisiones de identidad celular, incluyendo mielopoiesis, adipogénesis y el desarrollo embrionario temprano. La metiltransferasa de argininas Carm1 también está involucrada en los mismos procesos, pero todavía no se ha descrito una posible conexión con C/EBPα. En esta Tesis, hemos investigado la conexión entre éstas dos proteínas en decisiones de identidad celular. Hemos descubierto que la arginina 35 de C/EBPα es un blanco clave para la metilación por Carm1, y está modificación postraduccional altera las propiedades biológicas del factor y actúa como un interruptor de identidad celular en mielopoiesis, adipogénesis y en la primera decisión entre pluripotencia y trofectodermo en el embrión.
Чобіт, М. Р., В. Л. Костюк, В. П. Васильєв та Ю. В. Панченко. "Модифікація жировмісними відходами мінеральних наповнювачів". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/52779.
Повний текст джерелаMcDaniel, Lori Heldreth. "Investigation of Liquid Trapping Following Supercritical Fluid Extraction." Diss., Virginia Tech, 1999. http://hdl.handle.net/10919/29121.
Повний текст джерелаPh. D.
Bouhlal, Sofia. "Consequence of salt, sugar and fat content modifications in foods on children's preference and intake." Thesis, Dijon, 2011. http://www.theses.fr/2011DIJOS110/document.
Повний текст джерелаBackground: In France, the National Nutrition and Health Programme (“Programme National Nutrition Santé” or “PNNS”) is in charge of implementing the recommendations aiming at reducing salt, sugar and fat quantities in foods. However, the extent to which this might impact children’s food preferences and intake is limited. Objective: The present work aimed at understanding the impact of varying salt, sugar and fat variations in foods, on toddlers and children’s food preferences and intake. Methods: Studies were conducted in toddlers’ and children’s natural eating environment (nursery and school canteen), at their usually scheduled lunch and/or snack times. Results: Concerning sucrose, the addition of a low amount seems enough to trigger intake; thus, sucrose can be reduced without affecting food preferences and intake. As far as fat is concerned, suppressing or adding butter did not have an immediate impact on toddlers’ green beans or pasta intake. Besides, low-fat products seem to be well accepted by toddlers and adults. Concerning salt, its reduction seems more puzzling and should be considered cautiously. Our results highlighted dissociation between the direct positive effect of salt on food preferences and intake, and its role on learning. Conclusions: Food sensory qualities are important for eating behaviour. Although it seems that reductions of sugar and fat in foods are possible, thus helping to reduce energy intake, those concerning salt should be considered cautiously
Stoilova, Bilyana [Verfasser], Achim Akademischer Betreuer] Leutz, Klaus [Akademischer Betreuer] Rajewsky, and Thomas [Akademischer Betreuer] [Sommer. "Structural plasticity and post-translational modifications of C/EBP beta direct distinct myeloid cell fates / Bilyana Stoilova. Gutachter: Achim Leutz ; Klaus Rajewsky ; Thomas Sommer." Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://d-nb.info/103545095X/34.
Повний текст джерелаMaurel, Delphine. "Consommation chronique d'alcool, exercice physique et tissu osseux : modifications densitométriques, architecturales, biomécaniques et métaboliques chez le rat." Phd thesis, Université d'Orléans, 2011. http://tel.archives-ouvertes.fr/tel-00789948.
Повний текст джерелаPascart, Tristan. "Étude des modifications osseuses de composition et de structure au cours de l’ostéonécrose de tête fémorale et corrélations étiopathogéniques IRM." Thesis, Lille 2, 2017. http://www.theses.fr/2017LIL2S049/document.
Повний текст джерелаIntroductionThe pathophysiology and structural evolution of the osteonecrosis of the femoral head (ONFH) are poorly understood. The objectives of this thesis were to search for modifications of composition and structure of both the trabecular and the subchondral bone occurring during ONFH.MethodsA preliminary study looked into the potential modifications of bone composition induced by embalmment procedures using Raman spectroscopy in order to determine which controls are the most appropriate for comparisons with pathological bones. Femoral bones from 5 embalmed anatomical subjects were compared to femoral bones from 5 subjects that did not undergo any preservation procedure. The first work on ONFH addressed the potential modifications of trabecular bone mineral and organic physico-chemical parameters using Raman spectroscopy. The study included bone samples from femoral heads of 11 patients affected with ONFH and compared them to samples from 11 fresh anatomical subjects. The second study on ONFH addressed modifications of the subchondral bone. The study included 15 patients suffering from ONFH and 15 patients affected with hip osteoarthritis (OA) requiring joint prosthetic replacement. MRI examination that included standard medical sequences as well as experimental perfusion and DIXON sequences was performed prior to surgery. Once removed, the femoral heads were scanned using a nano-CT and samples of regions of interest were performed and analysed using histology, nano-CT with contrastenhancer marking and Raman spectroscopy.ResultsBone femoral mineral and organic composition was significantly altered in embalmed samples. Trabecular bone composition did not differ in any of the regions of interest inside the group of patients with ONFH nor in comparison with the control group of embalmed subjects after adjustment on age. The volume of the necrotic zone defined by the hypo-T1 MRI signal correlates to the volume including the sclerotic zone measured by nano-CT. Regarding the subchondral bone, preliminary results found differences of mineral and organic composition of the subchondral plate between zones of the ONFH group and with the OA group. Qualitative preliminary results from the study of biopsies analysed with the nano-CT and histology suggest that there are alterations of the subchondral plate and of the subchondral trabecular bone to a lesser extent. MRI examination with the DIXON sequence and nano-CT scans suggest that there is a decrease of the adipose content in the femoral head but an increase in the femoral neck in the ONFH group compared to the OA group.ConclusionThis work adds further evidence advocating that trabecular disorganization during ONFH is a consequence of massive alterations of the subchondral bone
Trebuchet, Guillaume. "Etude des mécanismes de la différenciation cellulaire impliquant le facteur de transcription Glide/Gcm chez la drosophile." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAJ114/document.
Повний текст джерелаCell fate determination involves key transcription factors. ln Drosophila, the transcription factor Glide/Gcm is required for the differentiation of two immune cell types: circulating macrophages,which arise from hematopoietic precursors, and glial cells, resident macrophages of the central nervous system, which differentiate from neural precursors. ln first, 1 characterized Gcm hematopoietic potential and identified its target genes in hemocytes. Then, to get an insight intomolecular mechanisms underlying the acquisition of several identities with a single fate determinant, 1 investigated how the choice between the hemocyte and the glial fates is regulated.Being necessary to consolidate and to maintain a specific fate, 1 highlight the key role of genes acting downstream of a fate determinant. Finally, 1 contribute to characterize Gcm expression profile at the protein level and highlight a new role of Gcm in the differentiation of neurosecretory cells, cells absolutely required to initiate the hormonal signal triggering the molting process in insects
Reddy, Yella S. "Studies on modification of fats of tree-origin for use in confectionery." Thesis, 1988. http://hdl.handle.net/2009/1963.
Повний текст джерелаSadeqzadeh, Elham. "Analysis of post-translational modifications of Fat1 cadherin." Thesis, 2014. http://hdl.handle.net/1959.13/1050575.
Повний текст джерелаFirst identified in Drosophila as a tumour-suppressor gene, Fat cadherin (Ft) and the closely related Fat2 (Ft2) have been identified as giant members of the cadherin superfamily. Ft engages the Hippo signalling pathway during development and both receptors have been shown to function in different aspects of cell polarity and migration. There are four vertebrate homologues, Fat1-Fat4, all closely-related in structure to Drosophila ft and ft2. Over the past decade knock-out mouse studies, genetic manipulation and large sequencing projects have aided our understanding of the function of vertebrate Fat cadherins in tissue development and disease. The majority of studies of this family have focused on Fat1, with evidence now showing it can bind to ENA/VASP, β-catenin and Atrophin proteins to influence cell polarity and motility; Homer1 and 3 proteins to regulate actin accumulation in neuronal synapses; and Scribble to influence the Hippo signalling pathway. Fat2 and Fat3 can regulate cell migration in a tissue specific manner and Fat4 appears to influence both planar cell polarity and Hippo signalling recapitulating the activity of Drosophila Ft. Knowledge about the exact downstream signalling pathways activated by each family member remains in its infancy, but it is becoming clearer that each may have tissue specific and redundant roles in development. Importantly there is also evidence building to suggest that Fat cadherins may be lost or gained in certain cancers. This thesis represents the first in-depth biochemical investigation of human FAT1 cadherin, particularly its comparative expression in normal versus cancer cells. The first chapter studied the expression profile of all FAT cadherins in a panel of 20 cultured melanoma cells where all melanoma cell lines variably, but universally express FAT1 at the mRNA level and less commonly Fat2, Fat3 and Fat4. Both normal melanocytes and keratinocytes also express comparable FAT1 mRNA levels relative to melanoma cells. Analysis of the protein processing of FAT1 in keratinocytes revealed that human FAT1 was site-1 (S1) cleaved into a non-covalent heterodimer before achieving cell surface expression. A similar processing event had been reported in Drosophila Ft indicating that this was an evolutionary conserved mechanism. The use of inhibitors also established such cleavage is catalysed by a member of the proprotein convertase family, likely furin. However, in melanoma cells the non-cleaved pro-form of FAT1 was also expressed on the cell surface together with the S1-cleaved heterodimer. The appearance of both processed and non-processed forms of FAT1 on the cell surface demarked two possible biosynthetic pathways. Moreover FAT1 processing in melanoma cells generated a potentially functional proteolytic product in melanoma cells: a persistent 65kDa membrane-bound cytoplasmic fragment no longer in association with the extracellular fragment. Localisation studies of FAT1 both in vitro and in vivo showed melanoma cells display high levels of cytosolic FAT1 protein whereas keratinocytes, despite comparable FAT1 expression levels, exhibited mainly cell-cell junctional staining. The mechanisms deriving the unprocessed FAT1 and the p65 product were then further investigated to uncover the potential biological activities of these cancer specific products. The second chapter investigated the mechanisms behind dual processing of FAT1 in cancer cells including the mechanism of FAT1 heterodimerisation. Generally the S1 processing step and accompanying receptor heterodimerisation is thought to occur constitutively but the functional significance of this process in transmembrane receptors has been unclear and controversial. Using siRNA against a number of different proprotein convertases it was established that the S1-cleavage of FAT1 is catalysed only by furin. Mass spectrographic analysis identified the precise location of the cleavage site occurring between the laminin G and the second EGF domain on the extracellular domain of FAT1, consistent with an evolutionarily conserved region found in Drosophila DE-cadherin known to be involved in heterodimerisation. Utilising furin overexpressing studies in melanoma together with the furin deficient LoVo cells, indicated the likely reason behind partial heterodimerisation of FAT1 was deficiency in furin activity. Moreover, it was also determined from these experiments that only the heterodimer form of FAT1 was subject to a second cleavage step (S2) and subsequent release of the extracellular domain. This indicated that S1-processing was a prerequisite for FAT1 ectodomain shedding and established a general biological precedent with implications for the shedding of other transmembrane receptors that undergo heterodimerisation. Part of this work also established an ELISA assay against the extracellular domain of FAT1 that may find utility to investigate shed FAT1 as a potential new cancer biomarker in blood. Previous studies in Drosophila had shown that the interaction between Ft and its ligand, the large cadherin Dachsous (Ds) is regulated through ectodomain phosphorylation mediated by the atypical kinase, Four-jointed (Fj). The third chapter investigated the process of ectodomain phosphorylation of FAT1 on the basis that this important regulatory mechanism may be conserved. Using the known Fj-phosphorylation motif, in silico analyses were undertaken to determine if phosphorylation sites were conserved in human FAT cadherins. This search identified nine potential sites in FAT1 as potential substrates for the sole homologue of Fj in humans, FJX1. Using general antibodies against phospho-serine and phospho-threonine it was revealed that the extracellular domain of FAT1 was multiply phosphorylated on these residues. However, silencing FJX1 using either siRNA or stable shRNA transduction did not indicate any role for FJX1 in FAT1 ectodomain phosphorylation. Nevertheless, given that many regulatory processes are conserved between Drosophila and vertebrate Fat cadherins, the establishment that ectodomain phosphorylation occurs in FAT1 provides the strong likelihood that this process will be important in regulating the interaction of FAT1 with its presently unknown ligand. This knowledge may therefore provide an essential starting point for identifying the ligand of FAT1 and in helping to understand how their interaction is regulated between cells.
Rinas, Aimee Lynn. "Advancing the Applicability of Fast Photochemical Oxidation of Proteins to Complex Systems." Thesis, 2016. http://hdl.handle.net/1805/10991.
Повний текст джерелаHydroxyl radical protein footprinting coupled with mass spectrometry has become an invaluable technique for protein structural characterization. In this method, hydroxyl radicals react with solvent exposed amino acid side chains producing stable, covalently attached labels. Although this technique yields beneficial information, the extensive list of known oxidation products produced increases the complexity of identifying and quantifying oxidation products. The current methods available for quantifying the extent of oxidation either involve manual analysis steps, or limit the number of searchable modifications or the size of sequence database. This creates a bottleneck which can result in a long and arduous analysis process, which is further compounded in a complex sample. In addition to the data complexity, the peptides containing the oxidation products of hydroxyl radical-mediated protein footprinting experiments are typically much less abundant than their unoxidized counterparts. This is inherent to the design of the experiment as excessive oxidation may lead to undesired conformational changes or unfolding of the protein, skewing the results. Thus, as the complexity of the systems studied using this method expands, the detection and identification of these oxidized species can be increasingly difficult with the limitations of data-dependent acquisition (DDA) and one-dimensional chromatography. The recently published in cell FPOP method exemplifies where this field is headed - larger and more complex systems. This dissertation describes two new methodologies and one new technology for hydroxyl radical-mediated protein footprinting, expanding the applicability of the method. First is development of a new footprinting analysis method for both peptide and residue level analysis, allowing for faster quantification of results. This method utilizes a customized multilevel search workflow developed for an on-market search platform in conjunction with a quantitation platform developed using a free Excel add-in, expediting the analysis process. Second is the application of multidimensional protein identification technology (MudPIT) in combination with hydroxyl radical footprinting as a method to increase the identification of quantifiable peptides in these experiments. Last is the design and implementation of a flow system for in cell FPOP, which hydrodynamically focuses the cells, and when used yielded a 13-fold increase in oxidized proteins and 2 orders of magnitude increase in the dynamic range of the method.
Jesus, Diana Sofia Santiago. "Tailoring of the cellular microenvironment to guide cell fate and functions for bone tissue engineering strategies." Master's thesis, 2021. http://hdl.handle.net/10773/33110.
Повний текст джерелаThe ability of stem cells to differentiate into almost every cell type has been harnessed in tissue engineering strategies to direct their fate into specific lineages, through the mimicking of the extracellular matrix and microenvironment of tissues. For long, regenerative medicine approaches were almost exclusively dependent on the delivery of biochemical cues, but currently the role of mechanobiology in cellular behaviour has been increasingly clarified. Surface topography is one of the most well studied physical signal used for guiding cell fate. Different patterns have been shown to mediate several cellular events, including proliferation, migration, and differentiation. However, most research has been conducted in a two-dimensional context (2D), which does not truly mimic the native environment, while tri-dimensional (3D) constructs remain fairly unexplored. Furthermore, while synthetic polymers present tailoring advantages, they may hinder cell functions, including adhesiveness to substrates. In this work, we propose an integrative 3D approach, combining biochemical and physical stimulation, by a set of surface modifications applied to polycaprolactone microparticles (PCL). We evaluate their performance in mediating cellular behaviour, including cell adhesion and differentiation into the osteogenic lineage. Specifically, polycaprolactone microspheres were modified into discoidal microparticles presenting a nanogrooved surface pattern by imprinting, as confirmed by scanning electron microscopy, followed by coatings with poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenic protein- 2 (BMP-2). This technology allows more efficient delivery of biochemical signals, enabling the use of smaller concentrations, making it safer and less expensive than current approaches, allied to the topography effect. Furthermore, different growth factors may be bound instead of BMP-2, enabling the application of the modified microparticles to other target-tissues responsive to topography. Modified particles were seeded with human adipose-derived stem cells (hASCs) in static and dynamic conditions and used for a bottom-up assembly, in a 3D concept. Results showed that the modified microparticles can support cell viability and adhesion up to 21 days. In addition, the presence of osteogenic markers and important bone components suggest that, for themselves, topography and the biochemical coatings can promote differentiation of hASCs in the osteogenic lineage. Nonetheless, the overlap of the patterning dimensions and thickness of the PEA/FN/BMP-2 coating hampers the synergistic effect that we first expected. Finally, we trace future directions for the exploration of more biomimetic topographies.
Mestrado em Biotecnologia
Baril, Philip. "Étude théorique des mécanismes de transfert d'énergie suivant le passage d'un ion rapide sans un matériau." Thèse, 2008. http://hdl.handle.net/1866/8024.
Повний текст джерелаMachut, McLean. "Corrosion behavior of steels for lead-alloy cooled fast reactors and the effects of surface modifications on corrosion performance." 2007. http://catalog.hathitrust.org/api/volumes/oclc/152593766.html.
Повний текст джерелаTypescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 53-54).
Huang, Chen-Hsiu, and 黃振修. "Oocyte-induced extracellular matrix modification affected soma sexual fate from male to female phase in the protandrous black porgy, Acanthopagrus schlegelii." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/73113991881112992565.
Повний текст джерела國立臺灣海洋大學
水產養殖學系
104
Protandrous black porgy has a digonic gonad. After the E2 treatment, ectopic oocyte could be observed in the newly generated testis. Ectopic oocyte-expressing Figla levels highly-correlated to the Sertoli cells transdifferentiate into follicle-like cells. The ectopic oocytes were competent to create an ovarian microenvironment to protect against a testicular environment. The Amh released by ectopic oocyte-bordering Sertoli cells may suppress the proliferative activities of spermatogonia. Therefore, proliferation-arrested spermatogonia may act as a physical boundary to prevent the factors released by an ectopic oocyte-created female microenvironment to interfere with further ovarian development. The modification of heparan sulfate may keep specific factors in the extracellular matrix, we surmise that heparan sulfate help Amh gather in the nearby ectopic oocyte. In the present study, we collected abnormal testicular tissue contain ectopic oocyte from the ovotestis generating by feeding fish with E2 and then withdraw, as well as normal ovary tissue contain normal oocyte. The expression of glycosyl normal oocytes had no difference during 6-10 month of age, but had difference in the ectopic oocyte compared with normal oocytes by PAS staining. According to transcriptomic analysis of oocytes we found 4 genes in the heparan sulfate family. Base on qPCR results, the hs6st genes highly expressed in the ovary as compared with the testis. However, the hs6st genes are not related to sex differentiation. The gdf9 and the bmp15, belong to TGF-β superfamily members, are expressed in an oocyte-specific manner from a very early stage and play key roles in folliculogenesis. Furthermore, we select grb2 which has highly correlation with figla expression by transcriptomic analysis, and further confirmed by qPCR. The ectopic oocytes expressed stronger signals of bmp15, gdf9 and grb2 than the normal oocytes by in situ hybridization. Take together, our results support the hypothesis that the ectopic oocytes can create an ovarian microenvironment to protect against a testicular environment.
Wu, Ming-Feng, and 吳明鋒. "Modification and Implementation of an Edge-Based Fast Intra Prediction Mode Decision Algorithm for H.264/AVC High Resolution Real-time Systems." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/72087099617532834749.
Повний текст джерела國立交通大學
電機學院通訊與網路科技產業專班
96
In this thesis, we propose architecture of H.264/AVC fast intra prediction mode decision in high resolution real-time applications. Because the intra prediction mode decision occupied lots computations of the H.264/AVC video coding, besides it needs the extra time for modes generating of the intra prediction mode decision (about 20%), and the processed data is high dependence also, hence, it is a bottleneck of the high resolution real-time applications. In the intra prediction mode decision operations, we use an algorithm which based on the edge information of the object. It can reduce about 66% estimations of mode predictions with negligible loss of video quality and a little increasing of data rate. According to this algorithm, we propose a low cost architecture and the gate counts can be reduced about 50% to compare with the former design. The total gate counts are 86,671 only and the maximum operating frequency is 250 MHz. It is a favorable contribution to all the current high resolution real-time video processing while without spending a lot of hardware.
Sheibani, Ali. "The Effect of Salt Reduction, Culture and Process Modifications on the Physicochemical and Microbiological Characteristics of Cheddar-type Cheeses." Thesis, 2016. https://vuir.vu.edu.au/32884/.
Повний текст джерелаGupta, Rahul. "Influence of Chemical Coating on Droplet Impact Dynamics." Thesis, 2016. http://hdl.handle.net/2005/3262.
Повний текст джерела