Дисертації з теми "Expression study"
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1
Hettle, S. J. H. "Expression of TN1/3 transposase." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375435.
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Christine, Deborah. "The teaching of children's artistic expression." Thesis, The University of Arizona, 1988. http://hdl.handle.net/10150/276714.
Повний текст джерелаАнотація:
The development of Discipline-based Art Education (DBAE) has focused attention on curricular structure, especially as it relates to the concept of students' creative expression. Creative self-expression, the focus of many school art programs, is to encourage students' art production. Discipline-based art education in contrast strives to develop students' artistic expression. Achievement of artistic expression requires conceptually focused instruction of art content from four art disciplines, art history, art criticism, studio production, and aesthetics. A discipline-based lesson can be examined for the way artistic expression is fostered as a part of production. Specific examples drawn from one lesson are used to illustrate that artistic expression can be recognizable, sensitive to instruction, and subject to evaluation.
3
Wong, Hung-lai. "Gene expression study in ovary cancer." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B4073819X.
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黃虹麗 and Hung-lai Wong. "Gene expression study in ovary cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B4073819X.
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RODRIGUES, PAULA SALGADO LUCENA. "EXPRESSIVE TALKING HEADS: EXPRESSIVE TALKING HEADS: A STUDY ON SPEECH AND FACIAL EXPRESSION IN VIRTUAL CHARACTERS." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2002. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=6525@1.
Повний текст джерелаАнотація:
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFUNDAÇÃO PADRE LEONEL FRANCA
A face humana é interessante e desafiadora acima de tudo pela sua familiaridade. Essencialmente, é a parte do corpo utilizada para reconhecer indivíduos. Assim como a face, a fala é um importante instrumento na forma de comunicação do ser humano. Através da fala é possível externar pensamentos e, muitas vezes, ela indica o estado de ânimo em que uma pessoa se encontra. Juntos, fala e face são os principais elementos de interatividade entre os seres humanos. Contudo, reproduzir com naturalidade e fidelidade as peculiaridades destes dois elementos no universo computacional não é uma tarefa simples, constituindo-se em tópicos de pesquisa em diversas áreas, em particular na animação facial. Entre os diversos tipos de sistemas de animação facial, destacam-se como diretamente relacionados a este trabalho aqueles que envolvem a sincronização da fala de um personagem com a animação da sua face. Sistemas desse tipo são conhecidos como talking head ou talking face. Para o desenvolvimento de um sistema talking head, é necessário identificar as possíveis abordagens para a modelagem dos dois elementos básicos: fala e face. Os modelos utilizados irão influenciar não apenas a maneira como a animação é conduzida, mas a própria forma de interatividade do sistema. Uma contribuição importante deste trabalho é o estudo das possíveis abordagens e a proposta de uma taxonomia para a classificação de sistemas talking head. A partir da taxonomia proposta e fazendo uso de uma determinada abordagem para cada parâmetro analisado, foi desenvolvida uma aplicação que recebe como entrada um texto contendo a fala e anotações de expressividade e gera como saída, em tempo real, a animação de um personagem virtual enunciando o texto de entrada com o áudio e os movimentos faciais sincronizados. O sistema desenvolvido, denominado Expressive Talking Heads, explora a naturalidade da animação facial e ao mesmo tempo busca oferecer ao usuário uma interface com interatividade em tempo real. O Expressive Talking Heads pode ser executado tanto no modo isolado (stand alone) como acoplado a navegadores para a web, tendo sido projetado e desenvolvido com a preocupação de oferecer uma solução independente da plataforma e do sistema operacional utilizados.
The human face is interesting and challenging mainly because of its familiarity. Essentially, it is the part of the human body that is used to recognize individuals. As well as the face, the speech is an important instrument for human communication, allowing the exteriorization of thoughts and the definition of emotions. Together, speech and face are the main elements of interactivity among human beings. However, the natural and faithful reproductions of the pecularities of these elements in the computational universe is not a simple task, constituting topics of the research in the diverse areas, particularly in facial animation. Among the diverse types of facial animation systems developed, those that involve the facial animation of the virtual character combined with speech synchronization are distinguished as directly related to this work. These kinds of systems are known as talking head or talking face. Fot the development of a talking head system, it is necessary to identify the possible approaches for the speech and face modeling. The models used will influence not only the way that the animation is performed, but it will also affect the system´s interactivity. An important contribution of the present master thesis is the study of several possible approaches for the main elements and the proposal of taxonomy for the classification of the talking head systems. From the proposed taxonomy and making use of one approach for each analyzed paramenter, an application was developed that receives as input a text composed by the character´s speech and genus, language and emotion parameters, and it generates as output, in real time, the animation of a virtual character uttering the input text with speech synchronization and expressiveness. The system developed, called Expressive Talking Heads, explores the naturalness of facial animation and it seeks to offer the user a real- time interactivity interface. The Expressive Talking Heads system can run as a stand-alone applicattion or connected to web browsers. It was designed and developed to provide a platform and operating system independent solution.
6
Guo, Ran. "Subcloning, Expression, and Enzymatic Study of PRMT5." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/biology_theses/26.
Повний текст джерелаАнотація:
Protein arginine methyltransferases (PRMTs)mediate the transfer of methyl groups to arginine residues in histone and non-histone proteins. PRMT5 is an important member of PRMTs which symmetrically dimethylates arginine 8 in histone H3 (H3R8) and arginine 3 in histone H4 (H4R3). PRMT5 was reported to inhibit some tumor suppressors in leukemia and lymphoma cells and regulate p53 gene, through affecting the promoter of p53. Through methylation of H4R3, PRMT5 can recruit DNA-methyltransferase 3A (DNMT3A) which regulates gene transcription. All the above suggest that PRMT5 has an important function of suppressing cell apoptosis and is a potential anticancer target. Currently, the enzymatic activities of PRMT5 are not clearly understood. In our study, we improved the protein expression methodology and greatly enhanced the yield and quality of the recombinant PRMT5. In addition, mutagenesis and enzymatic studies implicate an interesting mechanism of PRMT5 activity regulation.
7
Devlin, Andrea. "A study of CYP1B1 expression in tumourigenesis." Thesis, University of Ulster, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494336.
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Fan, Wai-leong, and 樊偉亮. "Study of gene expression on ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44659039.
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Villette, Stephane. "Molecular study of selenoprotein in gene expression." Thesis, University of Newcastle Upon Tyne, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391327.
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Steel, Muriel C. "A study of muscarinic receptor gene expression." Thesis, University College London (University of London), 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314798.
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Yuwono, Triwibowo. "A study of actinidin expression in yeast." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/34380.
Повний текст джерелаАнотація:
Four different actinidin gene constructions have been created, each consisting of different functional parts of the actinidin gene: (1) the mature actinidin-coding DNA, (2) the amino-terminal extension, but without the secretion signal, plus the mature actinidin-coding DNA, (3) the mature actinidin plus the carboxy-terminal extension-coding DNA, and (4) the full-length precursor actinidin-coding DNA. The first three constructions were fused to the yeast MFa1 promoter and secretion leader sequence, while the fourth was coupled to the CYC1-GAL UAS promoter. Upon expression in yeast, no protein product was detected in the culture supernatant. Analysis of intracellular proteins showed that actinidin protein was detected only from the actinidin gene constructions which have the carboxy-terminal extensions, suggesting that the carboxy-terminal extension is required for the stability of the protein. Comparison of the actinidin proteins produced in protease-proficient and protease-deficient strains suggests that the processing of the protein requires the activity of vacuolar protease(s) and indicates that the actinidin was translocated into the yeast vacuole. Examination of the amino acid sequence suggested that actinidin possesses potential vacuolar and peroxisomal targeting signals. Since the actinidin precursor was glycosylated it must have entered the secretory pathway before being translocated into a specific cellular compartment. The HSP26 gene promoter has been shown to be induced by heat-shock and upon entry into stationary phase, thus it is potentially useful for heterologous gene expression in yeast.
12
Allen, Rebecca Louise. "A study of gene expression in Pseudomonas." Thesis, University of Leicester, 1989. http://hdl.handle.net/2381/35185.
Повний текст джерелаАнотація:
An inherent problem in the study of the genetics of the interesting and potentially commercially useful properties of the pseudomonads is that of gene expression, since many of the genes encoding these properties are not well expressed in an E.coli background. The evidence available at the present time indicates that some Pseudomonas genes may possess different promoter sequences not recognised by E.coli RNA polymerase. An in vitro coupled transcription/translation system based on P.putida has been developed. A comparison of E.coli and broad host range plasmid DNA in this and the equivalent E. coli system showed that although cloned E. coli and vector polypeptides were synthesised in both systems, there was a difference in the polypeptide products directed by broad host range plasmid DNA in the two systems. In particular RSF1010 directed the synthesis of a 73kD polypeptide uniquely in the P.putida system. This was shown to be a polypeptide involved in mobilisation of the plasmid. A broad host rsmge proraoter-probe vector based on RSFlOlO was constructed and used for the shotgun cloning of P.putida promoters. A small subset of fragments which were active as promoters in P.putida but exhibited much lower activity in E.coli were isolated, sequenced and analysed with respect to concensus E. coli and nitrogen-regulated promoter sequences. These isolated DNA fragments may represent promoters which have sequences specifically recognised by Pseudomonas RNA polymerase. An analysis of published Pseudomonas chromosomally-encoded promoters revealed putative Pseudomonas-specific concensus regions.
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White, David Robert. "Studies on the acquisition, expression and disruption of mammalian sperm motility." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/27046.
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Gonçalves, Ângela. "RNA sequencing for the study of gene expression regulation." Thesis, University of Cambridge, 2012. https://www.repository.cam.ac.uk/handle/1810/265548.
Повний текст джерелаАнотація:
The process by which information encoded m an organism's DNA is used in the synthesis of functional cell products is known as gene expression. In recent years, sequencing of RNA (RNA-seq) has emerged as the preferred technology for the simultaneous measurement of transcript sequences and their abundance. The analysis of RNA-seq data presents novel challenges and many methods have been developed for the purpose of mapping reads to genomic features and expression quantification. In the first part of my thesis I developed an R based pipeline for pre-processing, expression estimation and data quality assessment of RNA-seq datasets, which formed the basis for my subsequent work on the evolution of gene expression regulation in mammals. Since changes in gene expression levels are thought to underlie many of the phenotypic differences between species, identifying and characterising the regulatory mechanisms responsible for these changes is an important goal of molecular biology. For this, I studied the regulatory divergence of liver gene expression and of isoform usage between mouse strains. I demonstrate that gene expression diverges extensively between the strains and propose that the regulatory mechanism underlying divergent expression between two closely related mammalian species is a combination of variants that arise in cis and in trans. Isoform usage diverges to a lesser extent and appears to display a larger contribution of trans acting regulatory elements to its regulation, suggesting that isoform usage may be under different evolutionary constraints. These observations have important implications for understanding mammalian gene expression divergence and for understanding how speciation occurs.
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Ali, Norryai A. "Expression and secretion of OXA-2 beta-lactamase by Streptomyces lividans." Thesis, University of Surrey, 1986. http://epubs.surrey.ac.uk/847196/.
Повний текст джерелаАнотація:
The OXA-2 beta-lactamase gene was first found on a conjugative plasmid R46 from a clinical isolate of Salmonella typhimurium. To test the expression and secretion of OXA-2 beta-lactamase in Streptomyces lividans a shuttle plasmid (pSU101) was created by fusing an Escherichia coli plasmid (pSU8) carrying the OXA-2 beta-lactamase gene with the S. lividans vector pLJ61. The OXA-2 beta-lactamase gene specified by the hybrid plasmid PSU101 was expressed in S. lividans, although at a lower level than in coli. Almost all the beta-lactamase activity was found in the culture supernatant of S. lividans, whereas in E. coli the enzyme was almost wholly cell associated. The identity of the enzyme was established by substrate specificity and isoelectric focusing. The stability and integrity of the plasmid pSU101 in both E. coli and lividans was determined, in comparison with that of pSU8 and pLJ61 plasmids. The promoter regions of the OXA-2 beta-lactamase gene were identified by using promoter-probe plasmid vectors; and by S1 mapping of the transcriptional start-sites coupled to the DNA sequencing of the OXA-2 beta-lactamase gene. Multiple transcriptional start sites were found in both hosts, with the origin of transcription apparently different in the two organisms. Part of this work has been published as a scientific paper which is appended.
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Toufighi, Kiana 1980. "Integrative study of gene expression and protein complexes." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/380907.
Повний текст джерелаАнотація:
Over the last several decades, the emerging ‘integrated’ view of the cell has triumphed over
the ‘one gene/one protein/one function’ paradigm. This is illustrated by the biologically
opposite effects of key regulatory proteins in different cell types, in established versus
primary cells, and in vitro versus in vivo situations. The persistent theme throughout this
dissertation is the integration of a wide range of data sources for the purpose of
understanding distinct cellular contexts. We first use circadian expression data from human
epidermal stem cells to discover waves of transcripts expressed in tune with known clock
genes and show that time-of-day dependent responses to proliferation/differentiation cues is
important for skin homeostasis. We then combine this expression data with information on
protein structures and complexes to describe how protein-complex assembly is temporally
regulated during differentiation. Lastly, we show that human protein complexes are
composed of a stable ‘core’ and a plastic ‘periphery’ whose tissue-specific expression
allows protein complexes to function in a context-dependent manner.En las últimas décadas, la emergente vista integrativa de la célula ha triunfado sobre el paradigma histórico: ‘un gene/una proteína/una función’. Esto es ilustrado por los efectos biológicos opuestos de proteínas regulatorias clave en cultivos celulares inmortalizados frente a primarios e in vitro frente a in vivo. El tema persistente en este disertación es la integración de un amplio set de datos para estudiar los distintos contextos celulares. En primer lugar, utilizamos los datos de expresión génica obtenidos de células madre epidérmicas para descubrir las ondas de transcripción expresadas en sintonía con los genes conocidos de los ritmos circadianos. En este estudio demostramos que las respuestas de las células madres a las señales de proliferación/diferenciación dependen de hora del día y el tiempo circadiano es importante para la homeostasis de la piel. Posteriormente, combinamos estos datos de expresión con la información estructural de proteínas y complejos proteicos para describir la regulación temporal de complejos durante el proceso de diferenciación. Por último, mostramos que los complejos de proteínas humanos están compuestos de un ‘núcleo’ estable y una 'periferia' plástica cuya expresión específica de tejido celular permite que los complejos de proteínas funcionen de una manera dependiente del contexto.
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De, las Heras Rachel, and n/a. "Neuronal Differentiation: A Study Into Differential Gene Expression." Griffith University. School of Biomolecular and Biomedical Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040225.161725.
Повний текст джерелаАнотація:
Neuronal differentiation encompasses an elaborate developmental program which until recently was difficult to study in vitro. The advent of several cell lines able to differentiate in culture proved to be the turning point for gaining an understanding of molecular neuroscience. In particular the olfactory epithelium provides an attractive tool with which to investigate fundamental questions relating to neuronal differentiation, as it displays a unique capacity to regenerate and to retain a neurogenetic potential from its genesis and throughout adult life. The coordinated regulation of gene expression is fundamental to the control of neuronal differentiation. In order to reveal active processes at the molecular level and to dissect key components of molecular pathways, differential gene expression studies provide a foundation for the elucidation of dynamic molecular mechanisms. This thesis identified genes involved in neuronal differentiation by utilising a clonal olfactory receptor neuronal cell line (OLF442). Gene expression levels were identified using differential display and oligonucleotide array technology before and after serum deprivation. Differential display revealed two kinases whose expression levels were elevated during the differentiation of OLF442, identified as focal adhesion kinase (FAK) related non-kinase (FRNK) and mammalian ste20 like (MST)2 kinase. Furthermore, analysis of the oligonucleotide array data confirmed the expression of genes involved in altering presentation of extracellular matrix molecules, in mediating cytoskeletal rearrangements, and in ceasing the cell cycle, supporting the use of OLF442 as a model for studying differentiation. The differentiation of OLF442 results from the synchronisation of multiple transduction cascades and cellular responses as evidenced by the microarray data. A protein that can synchronise such signalling is the non-receptor protein tyrosine kinase, FAK. Thus the finding of the endogenous FAK inhibitor FRNK by differential display was intriguing as there was no difference in the expression level of FAK induced by differentiation, contrasting that of FRNK. This induced FRNK expression was derived autonomously as it was not responsive to the caspase-3 inhibitor, DEVD-CHO. This is particularly pertinent since the primary role of FRNK is to act as an inhibitor of FAK by competing with its substrates and reducing the phosphorylation of both FAK and its associated proteins. Differential display also revealed the upregulation of another kinase, which had 90% homology with rat MST2 kinase within the 3' UTR. Both mouse MST2 kinase (sequence submitted to GenBank, accession number AY058922) and the closely related family member MST1 kinase were sequenced and cloned. Moreover, evidence to support an autonomously expressed carboxyl-terminal domain of MST2 kinase is presented in Chapter 3 and provides a unique way in which MST2 may regulate its own activity. To further understand the role of MST in neuronal differentiation, a series of stable OLF442 transfections (with mutant and wild-type MST constructs) were carried out. MST was localised with cytoplasmic structures that may represent actin stress fibres, indicating a potential cytoskeletal role during neuronal differentiation. This indicated that MST1 may play a role in the morphological processes involved in neuronal differentiation. The identification of two kinases by differential display provided the motivation to understand the cellular context of OLF442 and to determine the phosphorylation status of the mitogen-activated protein kinase (MAPK) signalling cascades. Differentiation of OLF442 induced high-level phosphorylation of a putative B-Raf isoform, MEK2 and ERK1/2. Interestingly, there was a switch between preferential phosphorylation of MEK1 in undifferentiated OLF442 to preferential phosphorylation of MEK2 following differentiation. SAPK/JNK was also phosphorylated, as was the transcription factor c-Jun, which is a common substrate of both the ERK and SAPK/JNK signalling modules. The mapping of the cellular context of differentiating OLF442 has identified a promising model of a novel MAPK module. This consists of FAK signalling through Rap1 to ERK providing sustained activation, which is buffered or terminated by the expression of the endogenous FAK inhibitor FRNK. Furthermore, MST kinase could potentially play a role in regulating the cytoskeletal re-arrangements that are necessary for neuronal differentiation. MST kinase may signal transiently via the SAPK pathway to provide concomitant activation of c-Jun that is required for neuronal differentiation. An understanding of the gene expression pattern of the normal neuronal differentiation program allows a greater understanding of potential developmental aberrations. This could provide an opportunity for therapies to be conceived, while understanding the complexity of neuronal determination could also provide opportunities for stem cell transplantation.
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Sangaralingham, Sasantha Jeson. "Study of the gene expression during cardiac growth." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ55929.pdf.
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Wakeley, Philip Robert. "A study of maize male gametophytic gene expression." Thesis, Royal Holloway, University of London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261723.
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Choudhury, Tanzeem Khalid 1975. "FaceFacts : study of facial features for understanding expression." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/61109.
Повний текст джерелаАнотація:
Thesis (S.M.)--Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 1999.Includes bibliographical references (p. 79-83).
by Tanzeem Khalid Choudhury.
S.M.
21
Varanka, T. (Tuomas). "A comparative study of facial micro-expression recognition." Bachelor's thesis, University of Oulu, 2019. http://jultika.oulu.fi/Record/nbnfioulu-201909282947.
Повний текст джерелаАнотація:
Abstract. Facial micro-expressions are involuntary and rapid facial movements that reveal hidden emotions. Spotting and recognition of micro-expressions is a hard task even for humans due to their low magnitude and short duration compared to macro-expressions. In this thesis we look at why micro-expressions are important, datasets that contain micro-expressions for training of automatic systems, and how we can utilize modern computational methods to automatically recognize micro-expressions. Furthermore, we experiment with several representative methods in the literature and compare their performance.Vertaileva tutkimus mikroilmeiden tunnistuksesta. Tiivistelmä. Mikroilmeet ovat tahattomia ja nopeita kasvojen liikkeitä, jotka kertovat henkilön piilotetuista ilmeistä. Mikroilmeiden tunnistus ja luokittelu on vaikea tehtävä jopa ihmisille niiden lyhyen keston ja pienten liikkeiden takia verrattaessa makroilmeisiin. Tässä työssä tarkastelemme miksi mikroilmeet ovat tärkeitä, data-aineistoja, jotka sisältävät mikroilmeitä automaattisten systeemien opetukseen ja miten mikroilmeitä voidaan luokitella moderneilla laskennallisilla keinoilla. Lisäksi tarkastelemme ja testaamme eri keinoja kirjallisuudesta ja vertaamme niiden tuloksia.
22
Meyer, Eric C. "A visual scanpath study of facial affect recognition in schizotypy and social anxiety." Diss., Online access via UMI:, 2005.
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23
Mullarkey, K. "Gene expression in the salivary gland of the blowfly, (Calliphora vomitoria), larva." Thesis, University of Exeter, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382866.
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Bohlin, Stina. "Meaningless movement or essential expression : A study about gestures." Thesis, Luleå tekniska universitet, Institutionen för ekonomi, teknik, konst och samhälle, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-85247.
Повний текст джерелаАнотація:
The thesis investigates how body movements influence a musical performance, with the aim to reach a more expressive performance by an increased awareness of gestures. In the study, three versions of the same clarinet piece were recorded on video; one with me, one with my clarinet teacher and one with a fellow clarinet student. The study addresses the following research questions: - How do body movements correspond to musical intentions? - How are my gestures formed and influenced by my teachers’ gestures? - In what ways can a raised awareness of gestures affect my musical performance? The videos were coded and analysed using open coding. As a reference, each clarinettist notated their intended phrasing in the score. This was marked as phrases (slurs) and Goal Points, and was also annotated in ELAN. With the intention to answer the first research question, the body movements were compared with the performers intended phrasing. In order to answer the second research question, coded sequences from each performance were compared with each other to find similarities and differences, using both quantitative and qualitative methods. Finally, I recorded a second performance of the same piece to investigate whether awareness of gestures affected my performance. Results align with previous research and indicate that body gestures are unique for each performer and connected to musical intentions. Results also indicate a resemblance in movement patterns between my teacher’s performance and my own, suggesting that gestures can be transferred from teacher to student.
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Spauwen, Janneke, Lydia Krabbendam, Roselind Lieb, Hans-Ulrich Wittchen, and Os Jim van. "Does urbanicity shift the population expression of psychosis?" Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-108748.
Повний текст джерелаАнотація:
Growing up in an urban area has been shown to be associated with an increased risk of psychotic disorder in later life. While it is commonly held that a only a tiny fraction of exposed individuals will develop schizophrenia, recent evidence suggests that expression of psychosis in exposed individuals may be much more common, albeit at attenuated levels. Findings are based on a population sample of 2548 adolescents and young adults aged originally 14–24 years, and followed up over almost 5 years up to ages 17–28 years. Trained psychologists assessed all these subjects with the core psychosis sections on delusions and hallucinations of the Munich-Composite International Diagnostic Interview. Growing up in an urban area was associated with an increased risk of expression of psychosis in the adolescents and young adults (adjusted OR 1.31, 95% CI 1.03–1.66). The proxy environmental risk factor that urbanicity represents may shift a relatively large section of the adolescent population along a continuum of expression of psychosis. Other causal influences may be required to make the transition to schizophrenia in adult life.
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Bambrough, Marilyn Edna. "An Artful Habitat:Creating an Environment for Divergent Expression." BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/8798.
Повний текст джерелаАнотація:
Over the last century, teaching techniques and philosophies have changed extensively in the art classroom. Teaching methods have spanned a range that stretches from highly rigid, to self-expressive, more learner centered approaches. In recent years, there has been a resurgence of interest and research regarding the role of creativity in visual art education. This research project focused on the qualities of effective art instruction using elements from a number of historical ideologies, with the intent to study creative development in students. The research used a case-study methodology informed by a reflective, action research methodology. Research was used to determine effective engagement, and discover teaching strategies using fun and playful exploration that motivate students to be as creative as possible, discover what they are interested in, and engage them in their own artistic research. The application of this research is to inform and improve my own teaching practice, and to explore the qualities of effective learner centered art instruction for middle school age students.
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Li, Zhi 1959 Dec 23. "Cloning, expression and functional study of human hepadnaviral polymerase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0010/NQ59620.pdf.
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Whitehouse, Adrian. "An inducible expression system to study baculovirus gene function." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260142.
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Price, Rebekah. "Study of expression of multigene families in Plasmodium falciparum." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270241.
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30
Pappas, Beverly. "Mechanistic Study of p23-Mediated Aryl Hydrocarbon Receptor Expression." Scholarly Commons, 2018. https://scholarlycommons.pacific.edu/uop_etds/3131.
Повний текст джерелаАнотація:
The aryl hydrocarbon receptor (AHR) is a ligand-activated signaling molecule which is involved in diverse biological functions ranging from cancer metastasis to immune regulation. This receptor forms a cytoplasmic complex with Hsp90, p23, and XAP2. We have previously reported that down-regulation of p23 triggers degradation of the AHR protein, uncovering a potentially dynamic event which controls the cellular AHR levels without ligand treatment. Here we investigate the underlying mechanisms for this p23 effect using wild-type HeLa and the p23 knockdown HeLa cells. Reduction of the Hsp90 and XAP2 contents, however, did not affect the AHR protein levels, implying that this p23 effect on AHR is more than just alteration of the cytoplasmic complex dynamics. Association of p23 with Hsp90 is not important for the modulation of the AHR levels since exogenous expression of p23 mutants with modest Hsp90-binding affinity effectively restored the AHR message and protein levels. The protein folding property of p23 which resides at the terminal 50-amino acid region is not involved for this p23 effect. Results from our interaction study using the affinity purified thioredoxin fusion proteins and GST fusion proteins and isothermal titration calorimetry showed that p23 directly interacts with AHR and the interaction surface lies within AHR amino acid 1–216 and p23 amino acid 1–110. Down-regulation of the p23 protein content promotes the ubiquitination of AHR, indicating that p23 protects AHR from the ubiquitin-meditated protein degradation. However, the increased ubiquitination is not through the small ubiquitin-like modifier (SUMO) signaling pathway.
Troubleshooting and optimization were paramount for understanding and evaluating the p23 and AHR interaction. Specifically, the p23 mutant purification, p23: Hsp90 interaction, transient transfection, p23: AHR assay, and ITC study were phases of this research that required extensive time and critical thinking. These topics were further detailed to outline the specific problems encountered and the various steps taken to alleviate or optimize these issues.
31
Todd, C. B. "Septin 6 : A Study of Splicing, Expression and Stability." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501611.
Повний текст джерела
32
Torres, FÃbio Fernandes. "The gerund in future tense expression: a sociofunctionalism study." Universidade Federal do CearÃ, 2009. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3814.
Повний текст джерелаАнотація:
Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicoFundaÃÃo de Amparo à Pesquisa do Estado do CearÃ
This research deals with the variation of future tense with gerund forms in the Portuguese spoken in Fortaleza, from data of speakers of three different professions, collected by means of sociolinguistic interviews: salesmen, teachers and telemarketing workers. This phenomenon presents six variants: a) the imminent periphrastic simple future, b) the imminent periphrastic extended future - that compose the imminent future variable; c) the medium periphrastic simple future, d) the medium periphrastic extended future - that compose the medium future variable; e) the resultative periphrastic simple future, and f) the resultative periphrastic extended future - that compose the resultative future variable. We also consider a definition of gerundismo based on form, aspect, modality and temporal criteria. The theoretical referential is composed by the association of postulates of the Sociolinguistics Analysis and the Linguistic Functionalism, resulting in the theoretical configuration of the Sociofuncionalismo, considered by Tavares (2003). The analysis is performed in four stages: in the first one, we analyze the variants of imminent future; after that, the variants of medium future; later, the variants of resultative future and, finally, a section is dedicated for analysis of the variant called gerundismo. Results confirm that the phenomenon under study is influenced by factors of distinct nature: social factors, such as sex and profession of speakers and linguistic factors as type of the verb (auxiliary or modal verbs) and presence of one mark of future tense.
Esta dissertaÃÃo trata da variaÃÃo de tempo futuro no portuguÃs falado em Fortaleza codificado por perÃfrases gerundivas, a partir de dados de fala de informantes de trÃs diferentes Ãreas de atuaÃÃo, coletados por meio de entrevistas sociolinguÃsticas: vendedores, professores e operadores de telemarketing. Esse fenÃmeno apresenta seis variantes: a) futuro iminente perifrÃstico simples, b) futuro iminente perifrÃstico estendido â que compÃem a subvariÃvel futuro iminente; c) futuro mÃdio perifrÃstico simples, d) futuro mÃdio perifrÃstico estendido â que compÃem a subvariÃvel futuro mÃdio; e) futuro resultativo perifrÃstico simples e f) futuro resultativo perifrÃstico estendido â que compÃem a subvariÃvel futuro resultativo. Trata-se tambÃm da variante denominada gerundismo para a qual propomos uma definiÃÃo baseada em critÃrios tais como forma, aspecto, modalidade e natureza temporal. O referencial teÃrico à composto pela associaÃÃo de postulados da SociolinguÃstica Variacionista e do Funcionalismo LinguÃstico, resultando na configuraÃÃo teÃrica do Sociofuncionalismo, proposto por Tavares (2003). A anÃlise à feita em quatro etapas: na primeira, analisamos as variantes de futuro iminente; em seguida, as variantes de futuro mÃdio, depois, as variantes de futuro resultativo e, por Ãltimo, à dedicada uma seÃÃo para anÃlise da variante denominada gerundismo. Os resultados confirmam que o fenÃmeno em estudo à influenciado por fatores de uma natureza distinta: fatores sociais, tais como o sexo e a profissÃo dos falantes e fatores linguÃsticos como o tipo do verbo (verbos auxiliares ou modais) e presenÃa de uma marca de tempo futuro.
33
Tang, Mei Kuen. "Applications of the subtractive hybridization method to study gene expression in rat liver after cadmium exposure." HKBU Institutional Repository, 1994. https://repository.hkbu.edu.hk/etd_ra/39.
Повний текст джерела
34
Parker, Sydney Lawrence. "Temporality and its expression in the interlanguage of adult learners of Welsh." Thesis, University of Wales Trinity Saint David, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683050.
Повний текст джерела
35
Jia, Yizhen, and 贾亦真. "Bioinformatics study of the lineage and tissue specificity of genes and gene expression." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45540652.
Повний текст джерела
36
Lin, Shyr-Yeu 1962. "Genetically engineered mouse models for the study of follistatin biology." Monash University, Institute of Reproduction and Development, 2003. http://arrow.monash.edu.au/hdl/1959.1/5739.
Повний текст джерела
37
Tsiga, I. A. "To tell freedom : A study of black South African autobiography." Thesis, University of Essex, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377927.
Повний текст джерела
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Ball, Sarah Elizabeth. "A study of c-fms in myeloid leukaemias." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252976.
Повний текст джерела
39
Spauwen, Janneke, Lydia Krabbendam, Roselind Lieb, Hans-Ulrich Wittchen, and Os Jim van. "Does urbanicity shift the population expression of psychosis?" Technische Universität Dresden, 2004. https://tud.qucosa.de/id/qucosa%3A26767.
Повний текст джерелаАнотація:
Growing up in an urban area has been shown to be associated with an increased risk of psychotic disorder in later life. While it is commonly held that a only a tiny fraction of exposed individuals will develop schizophrenia, recent evidence suggests that expression of psychosis in exposed individuals may be much more common, albeit at attenuated levels. Findings are based on a population sample of 2548 adolescents and young adults aged originally 14–24 years, and followed up over almost 5 years up to ages 17–28 years. Trained psychologists assessed all these subjects with the core psychosis sections on delusions and hallucinations of the Munich-Composite International Diagnostic Interview. Growing up in an urban area was associated with an increased risk of expression of psychosis in the adolescents and young adults (adjusted OR 1.31, 95% CI 1.03–1.66). The proxy environmental risk factor that urbanicity represents may shift a relatively large section of the adolescent population along a continuum of expression of psychosis. Other causal influences may be required to make the transition to schizophrenia in adult life.
40
Bailey, Paul Charles. "A molecular study of dormancy-breaking in seeds of Trollius ledebouri." Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260658.
Повний текст джерела
41
Effio, Pedro Jorge Chimoy. "Estudo sobre a expressão dos genes de alfa amilase de Bacillus stearothermophilus e de anopheles merus em células de bactéria, levedura e inseto." Universidade de São Paulo, 2001. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-25102018-171719/.
Повний текст джерелаАнотація:
O gene A1 da alfa amilase foi isolado de uma biblioteca genômica em lambda EMBL3, feita com DNA do díptero primitivo Anopheles merus (Díptera, Nematocera, Culicoidea). Ele foi parcialmente seqüenciado, caracterizado pelo padrão da clivagem das enzimas restritivas e clonado no plasmídeo pIBI24 por Pernasetti (1991 ). No presente trabalho relata-se sua expressão em: bactéria (Escherichia coli AD494), células de inseto ( Spodoptera frugiperda) e em levedura (Pichia pastoris), tendo-se observado que somente nas células de Spodoptera frugiperda a enzima é expressa com atividade. A expressão extracelular do gene A1 foi ensaiada em E.coli AD494 usando o vetor pRSETc. Para este fim a seqüência do gene contendo seu próprio peptídeo sinal, foi inserida em fase de leitura (\"frame\") nesse vetor. A expressão sem peptídeo sinal foi executada usando o vetor pAE2, uma construção derivada- do pRSETc. Numa outra construção, o gene A1 foi inserido em fase de leitura após a seqüência do promotor e do peptídeo sinal do gene A2 (alfa amilase de Bacillus stearothermophilus) utilizando-se o vetor pIBI24-Bst. As células de E.coli AD494 transformadas com estes construções expressaram a enzima sem atividade. A amilase A1 não foi secretada nem com seu peptídeo sinal nem com o da amilase A2. A expressão do gene A1 em levedura (Pichia pastoris) foi feita usando o vetor pPic9, que permite a expressão tanto intracelular quanto a secreção da proteína. O gene foi amplificado por PCR usando \"primers forward\" com o intuito de obter o gene com ou sem peptídeo sinal. O gene contendo o peptídeo sinal foi construído com diferentes seqüências tipo Kozak precedendo o códon ATG. Para a expressão extracelular usou-se o peptídeo sinal do fator alfa do pPic9. Os resultados mostraram que a enzima é expressa mas que permanece dentro da célula sem atividade. As mesmas construções do gene A1 feitas para Pichia foram inseridas em Baculovírus para transfectar células de Spodoptera frugiperda. Neste sistema a amilase foi expressa com atividade principalmente no sobrenadante das culturas transformadas com construções usando o gene contendo o seu próprio peptídeo sinal assim com o peptídeo sinal da amilase de Zabrotes subfasciatus. O gene da alfa amilase de Bacillus stearothermophilus foi expresso no sistema Baculovírus-Spodoptera e na levedura Pichia, usando o gene contendo o peptídeo sinal da amilase de Z. subfasciatu. A proteína foi secretada e tinha atividade em ambos os sistemas.The alpha amylase A1 gene was isolated trom a genomic library of lambda EMBL3 made with DNA from the primitve Díptera Anopheles merus (Díptera, Nematocera, Cuclicoidea). This gene was partialy sequenced, characterized by standard restriction enzyme cleavage, and cloned into the pIBI24 plasmid by Pernasetti (1991). Here we present the expression of this gene in: bacteria (Escherichia coli AD494), insect cell (Spodoptera frugiperda) and yeast (Pichia pastoris). It was shown that only in Spodoptera frugiperda cells the protein display enzymatic activity. The presence of the A1 product in the extracelullar culture media was tested in Escherichia coli AD494 using the vector pRSETc. To this end, the gene sequence containing its own signal peptide was inserted in frame into the vector. Expression without a signal peptide was conducted using the pAE2 vector, a construct derived from pRSETc. In another construct, the A1 gene was inserted in frame after the promoter sequence and the signal peptide of the A2 gene (alpha amylase Bacillus stearothermophilus) using the pIBI24-Bst vector. Although the E.coli AD494 cells transformed with these constructs expressed the enzyme, no enzyme activity was detected. A1 was not secreted, either with its own signal peptide or with that of amylase A2. Expression of the A1 gene in yeast (Pichia pastoris) was conducted using the pPic9 vector, wich allows intracellular expression or secretion of this protein. The gene was amplified by PCR with forward primers, so as to amplyfy the gene sequence with or without the signal peptide. The gene containing the signal peptide was constructed with different Kozak sequences preceeding the ATG codon. For extracellular expression, the signal peptide of the pPic9 alpha factor was used. The results showed that the enzyme is expressed , but remained within the cell and do not display activity. The same constructs of the A1 gene made for P. pastoris were inserted in Baculovirus to infect Spodopera frugiperda cells. In this system, the amylase was successfully expressed and display enzymatic activity, mainly in the extracellular supernatant, using constructs of the gene with own signal peptide or with the signal peptide for amylase of Zabrotes subfasciatus. The gene A2 was expressed in the Baculovirus/Spodoptera system and the yeast P. pastoris using constructs containing the signal peptide of alpha amylase of Z. subfasciatus, the protein was secreted with activity.
42
Morten, Karl J. "A study of La France disease in Agaricus bisporus." Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254387.
Повний текст джерела
43
Sodekawa, Hiromi. "Enchi Fumiko : a study in the self-expression of women." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28285.
Повний текст джерелаАнотація:
This thesis examines four major works of Enchi Furaiko in terras of themes, style, and plot development. In these works, Enchi created three "types" of female characters: the vengeful woman, the lovable woman, and the elderly woman facing death and aging. She attempted to show how it was possible for these women, all repressed by a society, to release themselves from suppression to express their hidden, real selves. In exploring these issues, Enchi drew heavily on her knowledge of the Japanese classics, especially The Tale of Genji and late Edo fiction (including Kabuki), creating a literary world in which the classical and the modern, the past and the present were conflated. Unable to express their true selves within the constraints of a repressive social order, her characters seek self-expression and Eros through the intervention of mediumistic, spiritual, and supernatural forces. In Enchi's works, when the characters released spirits united with their Eros, they realized their essential femininity. An analysis of four of Enchi's major works clarifies these themes and Enchi's literary world.
Chapter One examines The Waiting Years, the work which established Enchi's reputation as a powerful novelist. Though marred by a lack of realism in the supportive characters, The Waiting Years succeeds in portraying a "vengeful woman" who expresses her essential femininity through revenge. A well-controlled, repressive style, influenced by that of The Tale of Genji and late Edo fiction, reinforces the theme of revenge and repression.
In contrast to this vengeful woman, Tale of the Mediums, which is analysed in Chapter Two, deals with the "lovable woman." This type of woman uses her spirit force to express her suppressed love. This chapter attempts to explain how Enchi employs complicated stylistic devices and a plot in which historical facts and fiction, present and past, and illusion and reality are conflated, in order to describe an ideal love. Tale of the Mediums, which can be called Enchi's work of Heian literature, creates a highly sophisticated and even a slightly artificial literary world.
Chapter Three focuses on the novel, Wandering Souls, which is part of the larger trilogy also called Wandering Souls. In this work, the heroine is neither a vengeful nor a loving woman. Although she is involved with men, love, and sex, she is forced to face the realities of aging, death, fear and loneliness. These harsh realities force her to release her hidden self from the forces of social suppression and from the barrier of her public self. Her self-expression takes place through the fusion of reality and illusion, in a world associated with that portrayed in The Tale of Genji.
The Mist in Karuizawa, Enchi's most mature work, is the subject of Chapter Four. All of Enchi's major concerns are brought into focus in this work. Using an imaginary classical work as the center of the novel, Enchi develops two additional narrative lines to create a sophisticated, layered plot. The heroine is an elderly woman facing aging, death, fear and loneliness, and her self-liberation takes place in an illusional world created through reference to the Japanese classics. In this work an ancient high priestess symbolizes the essential quality of femininity, the unity of spirit force and Eros, and through a supernatural relationship with this priestess, the novel's protagonist also realizes her essential femininity and life force.
This thesis, through the four works that are examined, can be considered an attempt to shed light on the question how Enchi's women characters express their hidden, real selves; it also attempts to assess Enchi's place as a modern Japanese writer.Arts, Faculty of
Asian Studies, Department of
Graduate
44
Han, Jiahuai. "Study of the regulation of cachectin/tumor necrosis factor expression." Doctoral thesis, Universite Libre de Bruxelles, 1990. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213139.
Повний текст джерела
45
Baken, Kirsten Annika. "Immunotoxicogenomics gene expression profiling as a tool to study immunotoxicity /." [Maastricht] : Maastricht : Universitaire Pers Maastricht ; University Library, Universiteit Maastricht [host], 2007. http://arno.unimaas.nl/show.cgi?fid=9329.
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46
Stern, Kalyn Michiko. "Immunohistological study of sorting nexin 27 expression in rat brain." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p1453421.
Повний текст джерелаАнотація:
Thesis (M.S.)--University of California, San Diego, 2008.Title from first page of PDF file (viewed July 14, 2008). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 88-94).
47
Grant, A. "The hippocalcin gene : a study in neuron-specific gene expression." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599607.
Повний текст джерелаАнотація:
The regulation of gene expression in differentiated neurons is poorly understood. By studying the transcriptional regulation of particular "model genes", whose expression is neuron specific, we hope to gain insight into the mechanisms governing neuron specific gene expression as a whole. The rat hippocalcin gene encodes a small calcium binding protein. Northern blot analysis and an in-situ hybridization study demonstrated that rat hippocalcin gene expression is brain specific, with different neuronal populations transcribing the gene to varying extents. Particularly high hippocalcin mRNA levels are found in the hippocampus, caudate putamen and olfactory tubercle of the rat brain. RT-PCR analysis detected hippocalcin gene expression in neuronal x glial hybridoma (NG108) and rat pheochromocytoma (PC12) cell lines; however not in 3T3 fibroblast (3T3) or human embryonic kidney (HEK) cell lines. To investigate the regulation of this cell-specific expression, the rat hippocalcin gene was cloned and it's structure determined. Genomic regions were then tested for transcriptional activity in transgenic mice. 3.2 kb of the hippocalcin gene upstream region directs neuron-specific expression of a lacZ reporter gene, in a pattern partially corresponding with endogenous hippocalcin gene expression. Reporter gene expression was invariably observed in the hippocampus, suggesting the presence of a hippocampal-specific-cis-regulatory element. Transcriptional activity of the -3.2 to +1 kb hippocalcin upstream region was investigated in more detail using transient transfection of reporter constructs into neuronal (NG108 and PC12) and non-neuronal (3T3 and HEK) cell lines. This analysis identified three, cell-specific, positively activating, elements between -3.2 to 1.4 kb. One of these elements is a small 300 bp fragment, that independently directs high levels of neuronal-cell-line-specific reporter gene expression.
48
Leung, Cheuk-man, and 梁卓文. "A study of BARX2 expression in esophageal squamous cell carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B47560460.
Повний текст джерелаАнотація:
Background
Esophageal carcinoma mainly affects middle aged to elderly males. It ranks the
ninth most common cancer world-wide. The main histological types are squamous
cell carcinoma and adenocarcinoma. In Hong Kong, esophagus squamous cell
carcinoma (ESCC) is by far the more common. BARX2 is a human homeobox gene
located at 11q24-q25, encoding a protein of 254 amino acids. Recent researches show
that its expression in breast cancer promotes cellular invasion.
Objectives
The study aimed to test the hypothesis that BARX2 is a prognostic marker in
ESCC. BARX2 expression in ESCC was correlated with patient survival and other
clinicopathologic parameters in a cohort of patients.
Material and Methods
Records of ESCC patients were obtained retrospectively from the computerized
database of Queen Mary Hospital. ESCC patients, who underwent esophagectomy in
the hospital from 1998 to 2005 but without receiving prior chemotherapy or
radiotherapy directed to the tumor, were selected. Tumor staging was done according
to the 6th edition of AJCC Cancer Staging Manual. Immunohistochemical staining
for BARX2 expression was performed on paraffin sections of the primary ESCC
tissues sampled in a tissue microarray constructed for research purposes. The pattern
of BARX2 expression in nucleus and cell cytoplasm of tumor cells was recorded and
the staining intensity scored on a 4-point scale. The scores were statistically analyzed
together with the various clinicopathologic parameters. BARX2 expression and
patient survival time were analyzed by the log-rank test.
Results
A total of 78 ESCC patients were recruited. At the time of data analysis, 52
(66.7%) patients were dead. The overall median survival of patients was 14.3 months.
BARX2 was found to be mainly expressed in the cytoplasm of tumor cells while
non-tumor epithelium showed strong nuclear expression. Patients with high level
BARX2 expression had short survival time, though the difference did not reach
statistical significance (p=0.075). Within the subgroup of lower T-stage ESCC (T1-3),
high level BARX2 expression was significantly associated with shorter survival time
(p=0.042). However, differential BARX2 expression did not affect survival time
within the group of patients who had advanced stage (T4) disease (p=0.525). In
patients who had no regional lymph node metastasis (N0), high level BARX2
expression was associated with shorter survival time (p=0.023). However, when
patients had regional lymph node metastases (N1), BARX2 expression did not affect
patient survival time (p=0.533). Patients whose ESCC showed moderate
differentiation in a three-tier tumor grading system, when accompanied with low level
BARX2 expression, had longer survival time (p=0.029). However, BARX2
expression did not affect survival time when ESCC showed either well differentiation
(p=0.462) or poor differentiation (p=0.637). Multivariate analysis showed patient age
and T-stage to be the only two independent parameters of prognostic significance
(p=0.025 and p=0.036 respectively).
Conclusions
BARX2 expression in ESCC was aberrant and mainly cytoplasmic. It was
inversely correlated with patient survival time in early ESCC disease (T1-T3 or N0).
BARX2 expression evaluated by immunohistochemistry could be a useful and
practical prognostic marker of ESCC in its early stages, when the proper decision on
treatment would be critical for the patients.published_or_final_version
Pathology
Master
Master of Medical Sciences
49
曾騰霈. "Study on Robot Facial Expression." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/70225260118183508943.
Повний текст джерелаАнотація:
碩士建國科技大學
自動化工程系暨機電光系統研究所
99
The facial expressions of a robot were designed to have the robot look friendly. Propeller servo controller and Visual Basic Windows were applied for controlling actuators. The computer programs developed in this research may drive eyelids, mouth, and brows individually or simultaneously to create the facial expressions that include happiness, anger, depression and gladness. The results of this study made robots personified.
50
"Study of BRE expression and regulation." 2006. http://library.cuhk.edu.hk/record=b5896531.
Повний текст джерелаАнотація:
Tam Ka-ying.Thesis (M.Phil.)--Chinese University of Hong Kong, 2006.
Includes bibliographical references (leaves 164-179).
Abstracts in English and Chinese.
Chapter Chapter one: --- Introduction --- p.4
Chapter 1.1 --- Introduction of BRE --- p.4
Chapter 1.1.1 --- Discovery of BRE --- p.4
Chapter 1.1.2 --- cDNA sequence and amino acids sequence of BRE --- p.4
Chapter 1.1.3 --- BRE expression level in Human and rat organs --- p.5
Chapter 1.1.4 --- Expression of Human and mouse BRE in multiple isoforms --- p.6
Chapter 1.1.4.1 --- BRE isoforms in Human --- p.6
Chapter 1.1.4.2 --- BRE isoforms in mouse --- p.6
Chapter 1.1.5 --- mRNA level of BRE upon stress --- p.7
Chapter 1.1.6 --- BRE and steroidogenesis --- p.8
Chapter 1.1.7 --- BRE and p55 tumor necrosis factor α (TNF) receptor --- p.9
Chapter 1.1.8 --- BRE and NFkB activity --- p.9
Chapter 1.1.9 --- Anti-apoptotic effect of BRE --- p.10
Chapter 1.1.10 --- BRE enhances the growth of tumor cells --- p.12
Chapter 1.1.11 --- BRE and its ubiquitination activity --- p.12
Chapter 1.1.12 --- Regulation of Prohibitin and p53 expression and proliferation by BRE --- p.13
Chapter 1.2 --- Regulation of transcription --- p.15
Chapter 1.2.1 --- Cis-acting elements --- p.17
Chapter 1.2.1.1 --- The TATA box --- p.18
Chapter 1.2.1.2 --- The GC box and CAAT box --- p.18
Chapter 1.2.1.3 --- The initiator (Inr) --- p.19
Chapter 1.2.1.4 --- CpG islands --- p.20
Chapter 1.2.2 --- Trans- acting protein factors --- p.21
Chapter 1.2.2.1 --- Zinc finger domain --- p.21
Chapter 1.2.2.2 --- Basic helix-turn-helix domain (bHLH) --- p.22
Chapter 1.3 --- Hypothesis and Objectives --- p.23
Chapter Chapter two: --- Materials and Methods --- p.25
Chapter 2.1 --- Materials --- p.25
Chapter 2.1.1 --- Primers used in polymerase chain reaction (PCR) and sequencing --- p.25
Chapter 2.1.2 --- DNA clones used in the studies --- p.26
Chapter 2.1.3 --- Materials for DNA manipulation --- p.27
Chapter 2.1.4 --- Materials for protein manipulation --- p.28
Chapter 2.1.5 --- Antibodies --- p.28
Chapter 2.1.6 --- Chemical used in treatments --- p.29
Chapter 2.1.7 --- Kits --- p.29
Chapter 2.1.8 --- Culture media and reagents --- p.30
Chapter 2.1.9 --- Instrumentation --- p.30
Chapter 2.1.10 --- Bacterial strain used for transfection and cloning --- p.31
Chapter 2.2 --- Methodologies --- p.36
Chapter 2.2.1 --- Cell culture --- p.36
Chapter 2.2.1.1 --- Monolayer cells --- p.36
Chapter 2.2.1.2 --- Suspension cell --- p.36
Chapter 2.2.2 --- Identification of the transcriptional start site (TSS) of BRE by RNA ligase- mediated rapid amplification of 5，and 3，cDNA ends (RLM-RACE) --- p.37
Chapter 2.2.3 --- Preparation of the 5' untranslated region (UTR) fragments of BRE --- p.39
Chapter 2.2.3.1 --- Polymerase chain reaction (PCR) with Taq polymerase --- p.39
Chapter 2.2.3.2 --- Polymerase chain reaction (PCR) with PhusiońёØ high-fidelity DNA.… --- p.40
Chapter 2.2.4 --- Construction of the reporter constructs --- p.42
Chapter 2.2.5 --- Cell transfection --- p.42
Chapter 2.2.6 --- Dual-luciferase reporter assay --- p.43
Chapter 2.2.7 --- Western blotting --- p.44
Chapter 2.2.8 --- Cell cycle analysis by flow cytometry --- p.45
Chapter 2.2.9 --- BRE antibody production --- p.43
Chapter Chapter Three: --- Identification of transcriptional start sites and promoter region for BRE --- p.52
Chapter 3.1 --- Identification of the transcriptional start sites for BRE --- p.52
Chapter 3.2 --- Computational analysis of the 5' region of BRE --- p.57
Chapter 3.2.1 --- Putative transcriptional factor binding sites --- p.57
Chapter 3.2.2 --- CpG island --- p.58
Chapter 3.3 --- Identification of BRE promoter --- p.64
Chapter Chapter Four: --- Characterization of transcriptional regulation of BRE --- p.70
Chapter 4.1 --- Regulation of BRE promoter by genotoxic stimuli and retinoic acid --- p.71
Chapter 4.1.1 --- Etoposide --- p.71
Chapter 4.1.2 --- 4-nitroquinoline-l -oxide (4NQO) --- p.79
Chapter 4.1.3 --- Retinoic acid (RA) --- p.87
Chapter 4.2 --- Regulation of BRE promoter by p53 protein and gamma irradiation --- p.90
Chapter 4.2.1 --- Co-transfection with p53 plasmid --- p.90
Chapter 4.2.2 --- Gamma irradiation (y irradiation) --- p.96
Chapter 4.2.2.1 --- γ irradiation treatment of HeLa cells --- p.96
Chapter 4.2.2.2 --- γ irradiation treatment of Balb/c 3T3 cells --- p.99
Chapter 4.3 --- Regulation of BRE promoter by BRE --- p.103
Chapter 4.3.1 --- Co-transfection with V5-tagged BRE (GS-BRE) --- p.103
Chapter 4.3 2 --- Co-transfection with untagged BRE (pcDNA3-BRE) --- p.107
Chapter 4.4 --- Regulation of BRE promoter by culture condition --- p.110
Chapter 4.4.1 --- Cell density --- p.110
Chapter 4.4.2 --- Serum deprivation --- p.114
Chapter 4.5 --- Regulation of BRE promoter by kinase inhibitors --- p.120
Chapter Chapter Five: --- BRE and cell cycle analysis --- p.127
Chapter 5.1 --- Cell synchronization in G1 phase by aphidicolin (APC) --- p.127
Chapter 5.1.1 --- Flow analysis --- p.128
Chapter 5.1.2 --- Luciferase reporter assay --- p.128
Chapter 5.1.3 --- Western blot analysis --- p.129
Chapter 5.2 --- Cell synchronization in G2/M phase by colchicine (COL) --- p.137
Chapter 5.2.1 --- Flow analysis --- p.137
Chapter 5.2.2 --- Luciferase reporter assay --- p.137
Chapter 5.2.3 --- Western blot analysis --- p.138
Chapter 5.3 --- Cell cycle analysis of the treatments investigated by luciferase assays --- p.144
Chapter Chapter Six: --- Discussion --- p.149
Chapter 6.1 --- Study of BRE expression --- p.149
Chapter 6.2 --- Study of BRE regulation --- p.154
Chapter 6.3 --- Conclusion --- p.163
Reference --- p.164
Appendix (Raw data and statistical information of luciferase assays)