Готові списки джерел за темами / Ex vivo bone

Добірка наукової літератури з теми "Ex vivo bone"

Автор: Grafiati
Опубліковано: 10 грудня 2022
Оновлено: 28 січня 2023

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Статті в журналах з теми "Ex vivo bone"

1

Stirpe, Fiorenzo, Luigi Barbieri, Pier Luigi Tazzari, Angelo Dinota, and Marco Gobbi. "Ex vivo bone marrow purging with immunotoxins." European Journal of Haematology 43, S51 (April 24, 2009): 173–75. http://dx.doi.org/10.1111/j.1600-0609.1989.tb01512.x.

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Rutherford, R. B., B. Nussenbaum, and P. H. Krebsbach. "Bone morphogenetic protein 7 ex vivo gene therapy." Drug News & Perspectives 16, no. 1 (2003): 5. http://dx.doi.org/10.1358/dnp.2003.16.1.829301.

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FERNANDES, M. G., E. M. M. FONSECA, R. N. JORGE, M. VAZ, and M. I. DIAS. "THERMAL ANALYSIS IN DRILLING OF EX VIVO BOVINE BONES." Journal of Mechanics in Medicine and Biology 17, no. 05 (July 12, 2017): 1750082. http://dx.doi.org/10.1142/s0219519417500828.

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Bone drilling is a common procedure in Medicine, mainly in traumatology and orthopedic procedure for fractures fixation and in reconstructive surgery. The success of this surgical procedure is dependent on many factors, namely, on heat generation control during the bone drilling. The main concern in bone drilling is the mechanical and thermal damage of the bone induced by inappropriate parameters such as drill speed and feed-rate during the drilling. This study focuses on the temperature generated during drilling of cortical bone tissue (bovine origin) and solid rigid polyurethane foams with similar mechanical properties to the human bone tissue. Different parameters such as drill speed, feed-rate and hole depth were tested. All results showed that improvement of the drilling parameters and the drill temperatures can be estimated. It was concluded that when the drill speed and feed-rate were higher, the bone temperature increase was lower. The obtained results of temperature in the drilling process of polyurethane foam blocks or bovine bone were compared with a good agreement in between both.
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Cramer, E. E. A., K. Ito, and S. Hofmann. "Ex vivo Bone Models and Their Potential in Preclinical Evaluation." Current Osteoporosis Reports 19, no. 1 (January 11, 2021): 75–87. http://dx.doi.org/10.1007/s11914-020-00649-5.

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Abstract Purpose of Review Novel therapies for damaged and diseased bone are being developed in a preclinical testing process consisting of in vitro cell experiments followed by in vivo animal studies. The in vitro results are often not representative of the results observed in vivo. This could be caused by the complexity of the natural bone environment that is missing in vitro. Ex vivo bone explant cultures provide a model in which cells are preserved in their native three-dimensional environment. Herein, it is aimed to review the current status of bone explant culture models in relation to their potential in complementing the preclinical evaluation process with specific attention paid to the incorporation of mechanical loading within ex vivo culture systems. Recent Findings Bone explant cultures are often performed with physiologically less relevant bone, immature bone, and explants derived from rodents, which complicates translatability into clinical practice. Mature bone explants encounter difficulties with maintaining viability, especially in static culture. The integration of mechanical stimuli was able to extend the lifespan of explants and to induce new bone formation. Summary Bone explant cultures provide unique platforms for bone research and mechanical loading was demonstrated to be an important component in achieving osteogenesis ex vivo. However, more research is needed to establish a representative, reliable, and reproducible bone explant culture system that includes both components of bone remodeling, i.e., formation and resorption, in order to bridge the gap between in vitro and in vivo research in preclinical testing.
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Denis, I., G. Cournot, H. Lacroix, C. Colin, E. Zerath, and A. Pointillart. "In vivo bone metabolism and ex vivo bone marrow osteoprogenitors in vitamin D-deprived pigs." Bone 26, no. 5 (May 2000): 491–98. http://dx.doi.org/10.1016/s8756-3282(00)00257-x.

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Walker, Mary M., Molly E. Baumann, John H. Alexander, Britani N. Blackstone, Christopher B. Morgan, Thomas J. Scharschmidt, and Heather M. Powell. "Mechanical strain induces ex vivo expansion of periosteum." PLOS ONE 17, no. 12 (December 30, 2022): e0279519. http://dx.doi.org/10.1371/journal.pone.0279519.

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Segmental bone defects present complex clinical challenges. Nonunion, malunion, and infection are common sequalae of autogenous bone grafts, allografts, and synthetic bone implants due to poor incorporation with the patient’s bone. The current project explores the osteogenic properties of periosteum to facilitate graft incorporation. As tissue area is a natural limitation of autografting, mechanical strain was implemented to expand the periosteum. Freshly harvested, porcine periosteum was strained at 5 and 10% per day for 10 days with non-strained and free-floating samples serving as controls. Total tissue size, viability and histologic examination revealed that strain increased area to a maximum of 1.6-fold in the 10% daily strain. No change in tissue anatomy or viability via MTT or Ki67 staining and quantification was observed among groups. The osteogenic potential of the mechanical expanded periosteum was then examined in vivo. Human cancellous allografts were wrapped with 10% per day strained, fresh, free-floating, or no porcine periosteum and implanted subcutaneously into female, athymic mice. Tissue was collected at 8- and 16-weeks. Gene expression analysis revealed a significant increase in alkaline phosphatase and osteocalcin in the fresh periosteum group at 8-weeks post implantation compared to all other groups. Values among all groups were similar at week 16. Additionally, histological assessment with H&E and Masson-Goldner Trichrome staining showed that all periosteal groups outperformed the non-periosteal allograft, with fresh periosteum demonstrating the highest levels of new tissue mineralization at the periosteum-bone interface. Overall, mechanical expansion of the periosteum can provide increased area for segmental healing via autograft strategies, though further studies are needed to explore culture methodology to optimize osteogenic potential.
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Steck, R., C. Gatzka, E. Schneider, P. Niederer, and M. L. Tate. "Measurement of bone surface strains on the sheep metacarpus in vivo and ex vivo." Veterinary and Comparative Orthopaedics and Traumatology 16, no. 01 (2003): 38–43. http://dx.doi.org/10.1055/s-0038-1632754.

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SummaryBone surface strains were measured on the dorsal ovine metacarpus during normal locomotion on a treadmill at different walking speeds to determine physiological strain levels. These measured strains were related to the strains measured in an ex vivo model of the sheep forelimb with two types of load application: loading by two Schanz-screws and loading via the radius. In vivo, the average surface strains were found to be dependent upon body weight as well as the walking speed. The orientation of the peak principal strain corresponded to the longitudinal axis of the bone. Ex vivo, loads applied via Schanz screws in the screw-loading model lead to strains on the dorsal metacarpus that corresponds to strains experienced in vivo during intermittent peak loads. Screw loading imparted primarily a bending load to the metacarpus, with the dorsal aspect in compression and the palmar aspect in tension. Loads, applied via the radius and the hoof in the radius-loading model, resulted in bone surface strains comparable to those measured during slow walking in vivo. In both ex vivo loading situations, peak strain orientation was parallel to the longitudinal axis of the sheep metacarpus. In conclusion, the results show that although the ex vivo loading models do not exactly replicate the load experienced in vivo, the magnitude and orientation of the principal strains on the dorsal metacarpus are within the range of strains occurring during normal physiological loading. These data validate the physiological significance of the ex vivo model and aid in understanding effects of mechanical loading on interstitial fluid flow and mass transport through bone.
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Buduo, Christian A. Di, Alessandra Balduini, and David L. Kaplan. "Translational approaches to functional platelet production ex vivo." Thrombosis and Haemostasis 115, no. 02 (March 2016): 250–56. http://dx.doi.org/10.1160/th15-07-0570.

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SummaryPlatelets, which are released by megakaryocytes, play key roles in haemostasis, angiogenesis, immunity, tissue regeneration and wound healing. The scarcity of clinical cures for life threatening platelet diseases is in a large part due to limited insight into the mechanisms that control the developmental process of megakaryocytes and the mechanisms that govern the production of platelets within the bone marrow. To overcome these limitations, functional human tissue models have been developed and studied to extrapolate ex vivo outcomes for new insight on bone marrow functions in vivo. There are many challenges that these models must overcome, from faithfully mimicking the physiological composition and functions of bone marrow, to the collection of the platelets generated and validation of their viability and function for human use. The overall goal is to identify innovative instruments to study mechanisms of platelet release, diseases related to platelet production and new therapeutic targets starting from human progenitor cells.
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Giulivi, Antonio, Mike Halpenny, Paul Birch, Lin Yang, and Lisa Martin. "Ex-Vivo Expansion of Megakaryocyte Progenitors." Blood 104, no. 11 (November 16, 2004): 2885. http://dx.doi.org/10.1182/blood.v104.11.2885.2885.

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Abstract Following Bone Marrow Transplant, thrombocytopenia and neutropenia always occur and patients require additional post transplant support in the form of platelet transfusions. Megakaryocytes (Mk), the precursors of platelets, are contained in hematopoietic progenitor cell products but their number is variable and relatively low. The infusion of ex vivo expanded Mk precursors could be beneficial by shortening the time to platelet engraftment and therefore reducing the amount of platelet transfusion support required by bone marrow transplant patients. The objective of this project was to investigate the expansion of Mk progenitors from peripheral blood stem cell (PBSC) harvests from patients with haematological malignancies. Briefly, CD34+ cells were isolated and cultured in serum free media supplemented with thrombopoietin (TPO) and Interleukin 1 (IL-1) then incubated at 37°C /5% CO2 for 8 – 12 days. Megakaryocyte progenitor analysis was accomplished using flow cytometry analysis (CD34+/41+, CD41+, CD61+) and Mk culture analysis (CFU-Mk) (Stem Cell Technologies). Mk progenitor expansion efficiency was determined as “fold expansion” of Mk progenitors produced over input levels. After 8 days of culture, a mean expansion of 46 fold (range 1.2 – 327.0, n=10) in megakaryocytic cells (CD61+) and a 15 fold expansion (1.2 – 41.7, n=10) in megakaryocyte progenitor cells (CD34+/41+) was observed. After 12 days, a 116 fold expansion (1.5 – 286, n=7) in megakaryocytic cells (CD61+) and a 19 fold expansion (2.4 – 40, n=7) in Mk progenitors (CD34+/CD41+) was observed. This study demonstrates that CD34+ cells can be used to effectively expand megakaryocytic cells using just two cytokines for an incubation period of 8 – 12 days. This data could be used to develop future protocols for use in clinical applications.
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Davies, H. M. S. "Ex vivo calibration and validation of in vivo equine bone strain measures." Equine Veterinary Journal 41, no. 3 (March 2009): 225–28. http://dx.doi.org/10.2746/042516409x396317.

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Дисертації з теми "Ex vivo bone"

1

Yip, Stephen. "Ex vivo bone marrow purging using BPD-mediated photodynamic therapy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ34649.pdf.

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Smith, Emma Louise. "Development and characterisation of an ex vivo model system for bone repair." Thesis, Cardiff University, 2009. http://orca.cf.ac.uk/55910/.

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Limitations in current model systems for researching bone repair have hampered the development of alternative clinical therapies. This thesis aimed to develop and validate an ex vivo rat mandible model, to investigate specific molecular and cellular processes involved in bone repair. Maintenance of cell and tissue architecture and viability was shown within mandible slices cultured for up to 21 days, both intact and fractured. Autoradiographic studies showed that resident cells were actively synthesising and secreting proteins, and cells of the osteoblast lineage were shown to survive throughout the culture period. The model was responsive to exogenously added growth factors TGF-p1 and BMP-2, with increased cellular migration / proliferation and expression of bone matrix proteins observed. A second model system, an in vitro bone slab cell culture system, demonstrated that endogenous growth factors could be released from the matrix of bone by chemicals such as EDTA, calcium hydroxide, and sodium hydroxide. Different growth factor release kinetics were observed with each treatment, and released growth factors were capable of actively influencing the behaviour of osteogenic cells. Pre-treatment of mandible slices with these chemical treatments yielded similar results, with an observed increase in cell number, proliferation, and bone matrix protein expression. The ex vivo mandible model developed within this study may represent an ideal system for investigating specific processes of bone repair, as well as a promising alternative to in vivo testing of novel clinical therapeutics.
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Nahirnyak, Volodymyr M. "Ultrasound-Induced Hyperthermia in Ex VivoClotted Blood and Cranial Bone." Cincinnati, Ohio : University of Cincinnati, 2006. http://www.ohiolink.edu/etd/view.cgi?acc%5Fnum=ucin1155759458.

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Thesis (Ph. D.)--University of Cincinnati, 2006.
Title from electronic thesis title page (viewed Nov. 30, 2006). Includes abstract. Keywords: Ultrasound, hyperthermia, thrombolysis, clotted blood, ischemic stroke. Includes bibliographical references.
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Davies, Catrin Meleri. "Validation of an ex vivo, loaded, circumfusion culture for living cancellous bone explants." Thesis, Cardiff University, 2005. http://orca.cf.ac.uk/54559/.

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The goal of this project was to validate a novel, mechanically loaded culture system, for the maintenance of cancellous bone explants ex vivo. The Zetos system utilised cancellous biopsies (5 mm high, 10 mm diameter) from ovine distal femora, bovine distal metacarpals and human femoral heads loaded daily for 300 cycles, at 1 Hz, giving 4,000 microstrain. Prior to culture, qualitative evaluation of bone density and overall morphology was conducted. These tissues were highly variable with bovine tissue being the most homogenous with regards to density and that each species contained different ratios of red and yellow marrow. The viability of bone cells and matrix synthesis were analysed using a variety of techniques. The outcome of this study was that diffusion constraints were the major limitation of this system. Chamber design was not optimal for bathing the explants, which was inferior to submerged static culture in centrifuge tubes. Harvesting the tissue created damage to the bone core that resulted in a maximal volume loss of 36%, which also encouraged unwanted growth of a fibrous-like tissue over the explant periphery in a wound-like response, possibly enhanced by foetal calf serum in the media. Nevertheless, 3H-glycine incorporation detected proteins synthesised during day 7 and 14 of culture. Collagen was the predominant protein synthesised. Fluorochrome labelling demonstrated human bone apposition during culture, but was unsuccessful with bovine and ovine tissue. Mechanically loaded explants were qualitatively more viable than unloaded disuse explants and submerged static controls. These results demonstrate cell viability at least 15 days post-harvest. If the limitations can be improved, then there is potential for this system to become routinely used in bone research. This system would provide a future means to allow bone-biomaterial interactions and interfaces to be studied, reducing, refining and replacing the need for animal experimentation.
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Kiyan, Wataru. "Ultrasound Parameters for Human Osteoarthritic Subchondral Bone Ex Vivo: Comparison with Micro-Computed Tomography Parameters." Kyoto University, 2019. http://hdl.handle.net/2433/236621.

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Garcia, érika Fernanda Villamayor. "Análise biomecânica ex vivo de dois métodos de osteossíntese de pelve em cães." Universidade Federal de Santa Maria, 2010. http://repositorio.ufsm.br/handle/1/10070.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Approximately 25% of all fractures in dogs involve the pelvis, of which 18-46% are iliac fractures. Conservative treatment can be performed in simple cases where minimum displacement occurs fractured fragments. However when there is severe displacement of the fragments, pelvic canal narrowing and involvement of weight bearing, surgical fixation is indicated. A variety of techniques have been described for the iliac fracture fixation. The highest percentage of successful cases can be attributed to the use of plates. Other methods used include pins, cerclage wire and compression screw. This study evaluated biomechanically the use of cortical allografts preserved in honey for the stabilization of transverse osteotomy of the ilium in dogs, as well as the use of hemicerclage wire isolated this cases, and compared the of two methods against the forces of bending. Were prepared cortical bone implants removed from humerus of dogs that eventually died for reasons not related to this research. The implants were preserved in honey for a period between 30 and 128 days. Were tested bilaterally thirteen canines pelves which held the body transverse osteotomy of the ilium. One hemipelvis of each dog was stabilized with a bone graft fixed by two hemicerclage wire and the contralateral hemipelvis with hemicerclage wire alone. To test the strength of flexion was used a manual compression machine where hemipelvis each was mounted on a wooden support. It was established that the time to stop the application of bending force would be when the fissure of the fracture suffer traction until half the width of the ilium (TMLI) or to failure. The strength of flexion needed to TMLI was significantly higher (P = 0.03) for hemipelves stabilized with bone implants (mean ± SD: 16.54 ± 5.29 kg) than for hemipelves stabilized with hemicerclage wire used alone (mean ± SD: 12.54 ± 4.01 kg). The force applied to fail was also statistically higher (P = 0.002) for hemipelves stabilized with bone implants (mean ± SD: 20.16 ± 7.3 kg) than in stabilized with hemicerclage wire used alone (mean ± SD: 12.54 ± 4.01 kg). The results showed that the use of cortical bone implants is a viable alternative for fixing the iliac osteotomy and is more resistant to strength of flexion in relation to the use of hemicerclage wire used in isolation.
Aproximadamente 25% de todas as fraturas em cães envolvem a pelve, sendo que 18-46% são fraturas ilíacas. O tratamento conservador pode ser realizado em casos simples onde ocorre deslocamento mínimo dos fragmentos fraturados. Entretanto, quando há deslocamento grave dos fragmentos, estreitamento do canal pélvico e comprometimento do suporte de peso, a fixação cirúrgica é indicada. Uma variedade de técnicas tem sido descrita para a fixação de fraturas ilíacas. A maior porcentagem de casos de sucesso pode ser atribuída ao uso de placas. Outros métodos usados incluem pinos, cerclagem de fio de aço e parafusos compressivos. Este trabalho avaliou biomecanicamente o uso de um implante ósseo cortical alógeno preservado em mel para a estabilização de osteotomia transversa de ílio em cães, bem como o uso de hemicerclagem de fio de aço isoladamente nestes casos, e comparou os dois métodos de estabilização de ílio frente às forças de flexão. Foram confeccionados implantes ósseos corticais alógenos retirados de úmeros de cães que vieram a óbito por motivos não relacionados com este trabalho. Os implantes foram preservados em mel por um período entre 30 e 128 dias. Foram testadas bilateralmente 13 pelves caninas nas quais se realizou osteotomia transversa do corpo do ílio. Uma hemipelve de cada cão foi estabilizada com o implante ósseo fixado por meio de duas hemicerclagens de fio de aço e a hemipelve contralateral com hemicerclagem de fio de aço isoladamente. Para testar a força de flexão, foi utilizada uma prensa de compressão manual onde cada hemipelve foi montada em um suporte de madeira. Foi estabelecido que o momento de interromper a aplicação da força de flexão seria quando a fenda da fratura sofresse tração até a metade da largura do ílio (TMLI) ou até a falha. A força de flexão necessária para TMLI foi significativamente maior (P=0,03) para hemipelves estabilizadas com implante ósseo (média ± SD: 16,54 ± 5,29 kgf) do que para as hemipelves estabilizadas com hemicerclagem de fio de aço usada isoladamente (média ± SD: 12,54 ± 4,01 kgf). A força aplicada para falhar também foi estatisticamente maior (P= 0,002) para as hemipelves estabilizadas com implante ósseo (média ± SD: 20,16 ± 7,3 kgf) do que nas estabilizadas com hemicerclagem de fio de aço usada isoladamente (média ± SD: 12,54 ± 4,01 kgf). Os resultados demonstraram que o uso de implante ósseo cortical alógeno é uma alternativa viável para a fixação da osteotomia ilíaca e apresenta maior resistência à força de flexão em relação ao uso de hemicerclagem de fio de aço usada isoladamente.
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Drago, Manuela Aleluia. "Placa de osso bovino na osteossíntese de tíbia de coelhos: avaliação biomecânica ex-vivo." Universidade Federal do Espírito Santo, 2011. http://repositorio.ufes.br/handle/10/5097.

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Made available in DSpace on 2016-08-29T15:37:22Z (GMT). No. of bitstreams: 1 tese_5256_.pdf: 375211 bytes, checksum: 832eb7716f5f86a3d232c597e8e95ae1 (MD5) Previous issue date: 2011-09-06
O uso de materiais produzidos a partir de osso bovino tem sido proposto na confecção de implantes como pinos, placas e parafusos, por promoverem as mesmas funções de um enxerto ósseo, ou seja, serem osteoindutores e osteocondutores. Entretanto, aspectos estruturais e mecânicos devem ser estudados previamente ao uso in vivo de implantes de osso. Portanto, o objetivo desse estudo foi avaliar o comportamento mecânico, por meio do ensaio mecânico de flexão, de placas produzidas a partir osso cortical bovino, no reparo de fratura de tíbia de coelhos ex vivo. Para tal, 26 placas foram confeccionadas a partir de osso cortical bovino e conservadas em solução de sal a 150%. Foram utilizados três grupos para estudo: grupo GP (n=10), composto pelas placas ósseas; grupo GTP (n=16), tíbias de coelhos osteotomizadas e estabilizadas com placas ósseas e quatro parafusos; grupo GT (n=10), tíbias intactas. No ensaio biomecânico de flexão em três pontos, verificou-se a tensão máxima, deflexão máxima e rigidez. Os resultados foram submetidos ao teste de Kruskal-Wallis (p<0,05) e ao teste de Dunn. Comparando GT com o GTP, observou-se redução de 80% na tensão máxima. Também se notou redução de 87% na tensão máxima ao comparar GP com o GTP. Verificou-se que a placa de osso bovino possuiu maior tensão máxima que a tíbia do coelho. Houve redução a 52% na rigidez do GTP em relação ao GT. Não observou-se diferença significativa nesta propriedade entre GPT e GP. Observou-se diferença significativa entre os três grupos com relação à deflexão máxima, onde notou-se aumento de 100% e 30% nos grupos GTP e GP, respectivamente, em relação ao GT. Pode-se concluir que placas ósseas, no reparo de fratura de tíbia de coelhos ex vivo obtiveram propriedades mecânicas inferiores, quando comparada à tíbia intacta.
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Sadique, Faiqa M. "Feasibility of Ex Vivo Expansion, Transduction and Transplantation of Murine Bone Marrow Mesenchymal Progenitor/Stem Cells." Cincinnati, Ohio : University of Cincinnati, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=ucin1085741764.

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Ota, Tsuyoshi. "Administration of ex vivo-expanded bone marrow-derived endothelial progenitor cells attenuates focal cerebral ischemia-reperfusion injury in rats." Kyoto University, 2006. http://hdl.handle.net/2433/135643.

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10

Preston, Timothy. "Biomechanical comparison of dual bone fixation in an ex-vivo mid-diaphyseal fracture model of the feline radius and ulna." Thesis, Preston, Timothy (2015) Biomechanical comparison of dual bone fixation in an ex-vivo mid-diaphyseal fracture model of the feline radius and ulna. Masters by Research thesis, Murdoch University, 2015. https://researchrepository.murdoch.edu.au/id/eprint/27566/.

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This investigation was conducted in two phases. The first phase aim was to describe the length, internal and external diameter, cancellous bone volume/extent and cortical bone thickness at predetermined locations in the radius and ulna in a cohort of skeletally mature and disease-free cat cadavers using radiography and computed tomography. This phase provided a morphometric description of normal cat antebrachii and served as reference for implant selection for the second phase. The aim of the second phase was to compare the biomechanical properties of three constructs for dual bone fixation (DBF) in a cat ex vivo antebrachial, fracture-gap model. Twelve cat cadaver antebrachii were radiographed to confirm normal skeletal appearance and maturity. Antebrachii were allocated for application of one of three constructs in an incomplete randomised block design (n=8/group); 10-hole 1.5/2.0mm Synthes® LCP radial plate (P); Plate and 1.2mm ulna intramedullary pin (PI) and Plate with an 8-hole 1.5/2.0mm orthogonal Synthes® ulna LCP plate (PP). Dual bone fixation constructs were PP and PI with P used as a control. Constructs were tested in non-destructive mediolateral and caudocranial four point bending, axial compression and finally axial compression to failure. Dual bone fixation constructs (PI and PP) were significantly stiffer (P< 0.001) than P in axial compression and caudocranial bending. There was no difference between PI and PP in axial compression and caudocranial bending (P=0.28) and no difference between any construct in mediolateral bending (P=0.72). The failure load was significantly greater for PP than PI (P<0.001) and PP and P (P<0.001) respectively. There was no difference between PI and P failure loads (P=0.45). In this cat ex vivo fracture-gap model, DBF (PP and PI) constructs were significantly stiffer in axial compression and caudocranial bending than radial plate alone. Dual plate constructs had a significantly higher failure load than any other construct.
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Частини книг з теми "Ex vivo bone"

1

Goodyear, Simon R., and Richard M. Aspden. "Mechanical Properties of Bone Ex Vivo." In Methods in Molecular Biology, 555–71. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-415-5_35.

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Goodyear, Simon R., and Richard M. Aspden. "Mechanical Properties of Bone Ex Vivo." In Methods in Molecular Biology, 241–57. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8997-3_12.

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Bhat, Sunil, and Arun Singh Danewa. "Ex Vivo Manipulation of Stem Cell Product." In Contemporary Bone Marrow Transplantation, 1–16. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-64938-2_15-1.

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Bhat, Sunil, and Arun Singh Danewa. "Ex Vivo Manipulation of Stem Cell Product." In Contemporary Bone Marrow Transplantation, 483–98. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-36358-1_15.

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Nakahata, Tatsutoshi, Xingwei Sui, Sakura Tajima, Kohichiro Tsuji, Kiyoshi Yasukawa, Tetsuya Taga, and Tadamitsu Kishimoto. "Ex Vivo Expansion of Human Primitive Hemopoietic Progenitors." In Bone Marrow Transplantation, 47–63. Tokyo: Springer Japan, 1996. http://dx.doi.org/10.1007/978-4-431-68320-9_6.

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Staines, Katherine A., Genevieve Brown, and Colin Farquharson. "The Ex Vivo Organ Culture of Bone." In Methods in Molecular Biology, 199–215. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8997-3_10.

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Derocq, J. M., G. Laurent, P. Casellas, H. Blythman, and F. Jansen. "Techniques for Ex-Vivo Bone Marrow Treatment with Immunotoxins." In Targeting of Drugs, 93–101. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5574-8_8.

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Salih, Erdjan. "Ex-Vivo Model Systems of Cancer-Bone Cell Interactions." In Methods in Molecular Biology, 217–40. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8997-3_11.

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Vallera, Daniel A. "Immunotoxins for ex vivo bone marrow purging in human bone marrow transplantation." In Immunotoxins, 515–35. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1083-9_29.

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Scheding, Stefan, Wolfram Brugger, and Lothar Kanz. "Transplantation of Ex-Vivo Expanded Peripheral Blood Progenitor Cells After High Dose Chemotherapy in Cancer Patients." In Bone Marrow Transplantation, 250–57. Tokyo: Springer Japan, 1996. http://dx.doi.org/10.1007/978-4-431-68320-9_31.

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Тези доповідей конференцій з теми "Ex vivo bone"

1

Zilberberg, Jenny, Saba Choudhary, Eugenia Dziopa, Cirian Mannion, Yair Kissin, Erika Parasido, Christopher Albanese, and Woo Lee. "Abstract 1083A: An ex vivo 3D bone metastasis model for prostate cancer." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-1083a.

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2

Li, Yifang, Qinzhen Shi, Yuan Liu, Lingwei Shi, Meilin Gu, Xiaojun Song, Chengcheng Liu, and Dean Ta. "Ex-vivo Ultrasonic Tomography Imaging of Cortical Bone Based on Velocity Model Prediction." In 2021 IEEE International Ultrasonics Symposium (IUS). IEEE, 2021. http://dx.doi.org/10.1109/ius52206.2021.9593393.

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3

Peccarisi, Marco, Tommaso De Marco, Antonio Greco, Francesco Conversano, and Sergio Casciaro. "Ex-vivo measurements of quantitative ultrasound and micro-CT parameters on intact human femoral heads." In 2015 6th European Symposium on Ultrasonic Characterization of Bone (ESUCB). IEEE, 2015. http://dx.doi.org/10.1109/esucb.2015.7169891.

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4

De Tommasi, Francesca, Martina Zaltieri, Emiliano Schena, Carlo Massaroni, Eliodoro Faiella, Rosario Francesco Grasso, Bruno Beomonte Zobel, Elena De Vita, Agostino Iadicicco, and Stefania Campopiano. "Temperature Monitoring During Microwave Thermal Ablation of Ex Vivo Bovine Bone: a Pilot Test." In 2020 IEEE International Workshop on Metrology for Industry 4.0 & IoT (MetroInd4.0&IoT). IEEE, 2020. http://dx.doi.org/10.1109/metroind4.0iot48571.2020.9138272.

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5

Coughlin, Thomas R., Matthew Haugh, Muriel Voisin, Evelyn Birmingham, Laoise M. McNamara, and Glen L. Niebur. "Primary Cilia Knockdown Reduces the Number of Stromal Cells in Three Dimensional Ex Vivo Culture." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14723.

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Анотація:
Mesenchymal stem cells (MSCs) are multipotent stromal cells that reside in the bone marrow and differentiate into connective cell lines, such as adipocytes and osteoblasts [1]. An appropriate balance of MSC differentiation toward adipocytes and osteoblasts is vital to bone homeostasis [6]. In vitro work demonstrates that differentiation of MSCs is influenced by mechanical stimuli [2, 3]. In a mouse model, the ratio of adipocytes to MSCs in the marrow was 19% lower compared to controls following treatment by low magnitude mechanical signals (LMMS) [4]. In mice, LMMS increased MSC number by 46% and the differentiation capacity of MSCs was biased towards osteoblastic compared to adipogenic differentiation [5]. Thus, mechanobiological stimuli may play an important role in maintaining balanced MSC differentiation.
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6

Stadelmann, Vincent A., and Dominique P. Pioletti. "Micromotions at the Bone Implant Interface Up-Regulate Osteoblasts-Mediated Activation of Osteoclasts in Ex Vivo Human Samples." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-175743.

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Анотація:
A radiolucent zone at the interface of bone and implants in total joint prosthesis is frequently observed in uncemented and cemented implants. This radiolucent zone, representing a fibrous tissue, is the result of the progressive deterioration of the bone surrounding implants. Fibrous tissues affect the implant fixation, increasing then the risk of aseptic loosening. Today, aseptic loosening is the most common cause of arthroplasties revisions.
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7

Khmelinskii, A., M. Baiker, X. J. Chen, J. H. C. Reiber, R. M. Henkelman, and B. P. F. Lelieveldt. "Atlas-based organ & bone approximation for ex-vivo μMRI mouse data: A pilot study." In 2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro. IEEE, 2010. http://dx.doi.org/10.1109/isbi.2010.5490209.

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8

Zhang, Siyuan, Zhiwei Cui, Lei Zhang, Xingguang Zhu, Tianqi Xu, Yuqiang Han, Supin Wang, Xijing He, and Mingxi Wan. "Feasibility of acoustic evaluation of thermal lesions at bone-soft tissue interface of an ex vivo bovine bone exposed to high-intensity focused ultrasound." In 2015 IEEE International Ultrasonics Symposium (IUS). IEEE, 2015. http://dx.doi.org/10.1109/ultsym.2015.0411.

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9

Wölfel, EM, AK Siebels, H. Mushumba, B. Wulff, K. Püschel, M. Amling, C. Glüer, and B. Busse. "Ex vivo analysis of microstructural bone parameters in tibia and femur in diabetes mellitus using high-resolution peripheral quantitative computed tomography." In 1. MuSkITYR Symposium. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1700646.

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10

King, Bonnie L., Marie Bammer, Irfan Ali-Khan, Jonathan W. Hardy, and Christopher H. Contag. "Abstract A48: Optical imaging model for monitoring dynamic interactions of breast cancer cells and bone tissues in ex vivo co-cultures." In Abstracts: AACR Special Conference on Tumor Invasion and Metastasis - January 20-23, 2013; San Diego, CA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.tim2013-a48.

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