Добірка наукової літератури з теми "EPSPS gene amplification"
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Статті в журналах з теми "EPSPS gene amplification"
Chatham, Laura A., Kevin W. Bradley, Greg R. Kruger, James R. Martin, Micheal D. K. Owen, Dallas E. Peterson, Jugulam Mithila, and Patrick J. Tranel. "A Multistate Study of the Association Between Glyphosate Resistance and EPSPS Gene Amplification in Waterhemp (Amaranthus tuberculatus)." Weed Science 63, no. 3 (September 2015): 569–77. http://dx.doi.org/10.1614/ws-d-14-00149.1.
Повний текст джерелаMalone, Jenna M., Sarah Morran, Neil Shirley, Peter Boutsalis, and Christopher Preston. "EPSPS gene amplification in glyphosate-resistant Bromus diandrus." Pest Management Science 72, no. 1 (May 6, 2015): 81–88. http://dx.doi.org/10.1002/ps.4019.
Повний текст джерелаChatham, Laura A., Chenxi Wu, Chance W. Riggins, Aaron G. Hager, Bryan G. Young, Gordon K. Roskamp, and Patrick J. Tranel. "EPSPS Gene Amplification is Present in the Majority of Glyphosate-Resistant Illinois Waterhemp (Amaranthus tuberculatus) Populations." Weed Technology 29, no. 1 (March 2015): 48–55. http://dx.doi.org/10.1614/wt-d-14-00064.1.
Повний текст джерелаGaines, Todd A., Alice A. Wright, William T. Molin, Lothar Lorentz, Chance W. Riggins, Patrick J. Tranel, Roland Beffa, Philip Westra, and Stephen B. Powles. "Identification of Genetic Elements Associated with EPSPS Gene Amplification." PLoS ONE 8, no. 6 (June 10, 2013): e65819. http://dx.doi.org/10.1371/journal.pone.0065819.
Повний текст джерелаKoo, Dal-Hoe, Rajendran Sathishraj, Bernd Friebe, and Bikram S. Gill. "Deciphering the Mechanism of Glyphosate Resistance in <b><i>Amaranthus palmeri</i></b> by Cytogenomics." Cytogenetic and Genome Research 161, no. 12 (2021): 578–84. http://dx.doi.org/10.1159/000521409.
Повний текст джерелаChen, Jingchao, Cuilan Jiang, Hongjuan Huang, Shouhui Wei, Zhaofeng Huang, Huimin Wang, Dandan Zhao, and Chaoxian Zhang. "Characterization of Eleusine indica with gene mutation or amplification in EPSPS to glyphosate." Pesticide Biochemistry and Physiology 143 (November 2017): 201–6. http://dx.doi.org/10.1016/j.pestbp.2017.09.012.
Повний текст джерелаPline-Srnic, Wendy. "Physiological Mechanisms of Glyphosate Resistance." Weed Technology 20, no. 2 (June 2006): 290–300. http://dx.doi.org/10.1614/wt-04-131r.1.
Повний текст джерелаChen, Jingchao, Hongjuan Huang, Chaoxian Zhang, Shouhui Wei, Zhaofeng Huang, Jinyi Chen, and Xu Wang. "Mutations and amplification of EPSPS gene confer resistance to glyphosate in goosegrass (Eleusine indica)." Planta 242, no. 4 (May 22, 2015): 859–68. http://dx.doi.org/10.1007/s00425-015-2324-2.
Повний текст джерелаSalas, Reiofeli A., Franck E. Dayan, Zhiqiang Pan, Susan B. Watson, James W. Dickson, Robert C. Scott, and Nilda R. Burgos. "EPSPS gene amplification in glyphosate-resistant Italian ryegrass (Lolium perenne ssp. multiflorum) from Arkansas." Pest Management Science 68, no. 9 (July 19, 2012): 1223–30. http://dx.doi.org/10.1002/ps.3342.
Повний текст джерелаGhanizadeh, Hossein, Andrew G. Griffiths, Christopher E. Buddenhagen, Craig B. Anderson, and Kerry C. Harrington. "A PCR plus restriction enzyme-based technique for detecting target-enzyme mutations at position Pro-106 in glyphosate-resistant Lolium perenne." PLOS ONE 16, no. 2 (February 2, 2021): e0246028. http://dx.doi.org/10.1371/journal.pone.0246028.
Повний текст джерелаДисертації з теми "EPSPS gene amplification"
Putta, Karthik. "Physical mapping of EPSPS gene copies in glyphosate resistant Italian ryegrass (Lolium perenne ssp. multiflorum)." Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/36183.
Повний текст джерелаDepartment of Agronomy
Randall S. Currie
Mithila Jugulam
Italian ryegrass (Lolium perenne L. ssp. multiflorum (Lam.) Husnot), one of the problem weeds of the US, evolved resistance to multiple herbicides including glyphosate due to selection in Arkansas (AR). Glyphosate is a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor and amplification of EPSPS gene, the molecular target of glyphosate confers resistance to this herbicide in several weed species, including Italian ryegrass from AR. The objective of this study was to determine the expression of EPSPS gene and protein as well as distribution of EPSPS copies on the genome of glyphosate-resistant Italian ryegrass (ARR) using a known susceptible Italian ryegrass (ARS) from AR. EPSPS gene copies and expression of ARR and ARS were determined using quantitative PCR with appropriate endogenous controls. EPSPS protein expression was determined using Western blot analysis. Fluorescence in situ hybridization (FISH) was performed on somatic metaphase chromosomes to determine the location of EPSPS copies. Based on qPCR analysis, ARR plants showed a wide range of 12 to 118 EPSPS copies compared to a single copy in ARS. EPSPS gene expression correlated with the gene copy number in both ARR and ARS. Individuals with high EPSPS copies showed high protein expression in Western blot analysis. FISH analysis showed presence of brighter EPSPS signals, distributed randomly throughout the genome of ARR individuals compared to a faint signal in ARS plants. Random distribution of EPSPS copies was previously reported in glyphosate-resistant Palmer amaranth. Overall, the results of this study will help understand the origin and mechanism of EPSPS gene amplification in Italian ryegrass.
Dillon, Andrew James. "Relationship between EPSPS copy number, expression, and level of resistance to glyphosate in common waterhemp (Amaranthus rudis) from Kansas." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/19147.
Повний текст джерелаAgronomy
Mithila Jugulam
Common waterhemp (Amaranthus rudis) is a problematic weed species of cropping systems throughout the Midwestern states, including Kansas. Recently, waterhemp populations from Kansas were found to have evolved resistance to the widely used herbicide glyphosate as a result of amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), the enzyme target of glyphosate. The objectives of this research were to 1) perform glyphosate dose-response study and determine the relationship between relative EPSPS genomic copies and EPSPS gene expression in glyphosate-resistant waterhemp, and 2) characterize the genomic configuration and distribution of EPSPS copies using florescence in situ hybridization (FISH) in three glyphosate-resistant waterhemp populations. Waterhemp populations from eastern Kansas were screened with 868 g ae haˉ¹ (field used rate) of glyphosate, and genomic DNA and total RNA was isolated from the survivors to determine the EPSPS genomic copies and EPSPS gene expression relative to the acetolactate synthase (ALS) gene using qPCR. Furthermore, waterhemp specific EPSPS probes were synthesized to perform florescence in situ hybridization (FISH) on these glyphosate-resistant plants. Results of these experiments indicate a positive correlation between level of glyphosate resistance, EPSPS copies, and their expression. As expected, a negative correlation was found between shikimate accumulation and EPSPS copies. Sequencing of the EPSPS gene showed no presence of the proline 106 mutation, which is known to be associated with glyphosate resistance suggesting that an insensitive EPSPS enzyme was not involved in the mechanism of glyphosate resistance. FISH analysis of resistant plants illustrated presence of amplified EPSPS copies on two homologous chromosomes, likely near the centromeric region. . This is the first report demonstrating a positive relationship between EPSPS copies and expressions, as well as chromosome configuration of EPSPS copies in glyphosate- resistant waterhemp from Kansas.
Borgato, Ednaldo Alexandre. "Identificação de Amaranthus palmeri, caracterização da resistência múltipla a herbicidas inibidores da ALS e da EPSPS e controle químico baseado no uso das novas tecnologias transgênicas." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-16052018-125757/.
Повний текст джерелаPalmer Amaranth (Amaranthus palmeri) is a weed species native to the United States, but it was reported in Brazil for the first time in 2015. Despite this population being resistant to EPSPS and ALS inhibitors, the molecular basis of its multiple resistance is unknown up to date. Because of this species introduction to Brazil, alternatives of management with the new herbicide-tolerant crops technologies need to be studied. The objectives of this research are to characterize the weed species introduced to Brazil, identify the mechanisms conferring resistance to ALS and EPSPS inhibitors herbicides, and to propose management approaches in environments with the new genetically modified herbicide-tolerant crops. A genotyping bioassay using genetic markers was developed to confirm that the species collected in the state of Mato Grosso (BR-R) is indeed A. palmeri and not A. tuberculatus, another dioceous species in the Amaranthus genus. Dose-response experiments and shikimate accumulation bioassay data indicate high level of resistance, with LD50 of 4,426 and 3,400 g glyphosate ha-1 in the first and second experiments, respectively, higher than the double rate tipically recommended to control it, and minimal accumulation in BR-R with 1 mM of glyphosate in treated plants in the leaf disks assay. BR-R also was resistanto to sulfonilurea and imidazolinone herbicides. The mechanism conferring resistance to glyphosate identified in this population was gene amplification, with increased EPSPS copy number - between 50 and 179 more copies in BR-R. Besides, two target-site mutations were identified in the ALS gene sequencing, W574L and S653N, conferring resistance to sulfonilureas and imidazolinones. The weed control experiment, overal, herbicide tank mixtures achieved higher levels of control. Therefore, this research confirms the introduction of A. palmeri to Brazil, as well as its multiple resistance to EPSPS and ALS inhibitor herbicides. Its control is more efficient with herbicide mixtures, which guarantees more susteinable use of the new herbicide-tolerant crop technologies.
Ngo, The Duc. "Biology and glyphosate resistance in Chloris truncata (windmill grass) and Chloris virgata (feathertop Rhodes grass) in southern Australia." Thesis, 2017. http://hdl.handle.net/2440/119095.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food & Wine, 2017