Добірка наукової літератури з теми "Epithelial thickne"
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Статті в журналах з теми "Epithelial thickne"
1
Wu, Yanan, and Yan Wang. "Detailed Distribution of Corneal Epithelial Thickness and Correlated Characteristics Measured with SD-OCT in Myopic Eyes." Journal of Ophthalmology 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/1018321.
Повний текст джерелаАнотація:
Purpose.To investigate the detailed distribution of corneal epithelial thickness in single sectors and its correlated characteristics in myopic eyes.Methods.SD-OCT was used to measure the corneal epithelial thickness distribution profile. Differences of corneal epithelial thickness between different parameters and some correlations of characteristics were calculated.Results.The thickest and thinnest part of epithelium were found at the nasal-inferior sector (P<0.05) and at the superior side (P<0.05), respectively. Subjects in the low and moderate myopia groups have thicker epithelial thickness than those in the high myopia group (P<0.05). Epithelial thickness was 1.39 μm thicker in male subjects than in female subjects (P<0.001). There was a slight negative correlation between corneal epithelial thickness and age (r=−0.13,P=0.042). Weak positive correlations were found between corneal epithelial thickness and corneal thickness (r=0.148,P=0.031). No correlations were found between corneal epithelial thickness, astigmatism axis, corneal front curvature, and IOP.Conclusions.The epithelial thickness is not evenly distributed across the cornea. The thickest location of the corneal epithelium is at the nasal-inferior sector. People with high myopia tend to have thinner corneal epithelium than low–moderate myopic patients. The corneal epithelial thickness is likely to be affected by some parameters, such as age, gender, and corneal thickness.
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Crespo Millas, Sara, José Carlos López, Elena García-Lagarto, Estibaliz Obregón, Denise Hileeto, Miguel J. Maldonado, and J. Carlos Pastor. "Histological Patterns of Epithelial Alterations in Keratoconus." Journal of Ophthalmology 2020 (July 30, 2020): 1–7. http://dx.doi.org/10.1155/2020/1468258.
Повний текст джерелаАнотація:
Purpose. The purpose of this study was to confirm the presence of specific patterns of epithelial response in corneal buttons from keratoconus patients. Methods. This was a retrospective and descriptive study. 90 penetrating keratoplasty specimens obtained from patients diagnosed with keratoconus were evaluated using bright-field microscopy. Morphologically identifiable characteristics including epithelial cell density and epithelial thickness were analyzed on hematoxylin and eosin- (H&E-) and periodic acid of Schiff- (PAS-) stained slides. Results. Three distinctive patterns of epithelial alteration of the central cornea were established. Pattern 3, in which the central epithelium was as thick as peripheral epithelium, was the commonest (44.4%), followed by the pattern 2, defined as central epithelium thinner than periphery epithelium (38.9%), and the uncommonest pattern was number 1, with central epithelium thicker than the periphery (16.7%). Conclusions. Three distinctive histologic patterns that could potentially have a diagnostic and prognostic value in keratoconus patients were found.
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Buffault, Juliette, Pierre Zéboulon, Hong Liang, Anthony Chiche, Jade Luzu, Mathieu Robin, Ghislaine Rabut, Marc Labetoulle, Antoine Labbé, and Christophe Baudouin. "Assessment of corneal epithelial thickness mapping in epithelial basement membrane dystrophy." PLOS ONE 15, no. 11 (November 25, 2020): e0239124. http://dx.doi.org/10.1371/journal.pone.0239124.
Повний текст джерелаАнотація:
Purpose To investigate the corneal epithelial thickness topography with optical coherence tomography (OCT) and its relationship with vision quality in epithelial basement membrane dystrophy (EBMD). Methods 45 eyes of EBMD patients, 26 eyes of dry eye (DED) patients and 22 eyes of normal subjects were enrolled. All participants were subjected to 9-mm corneal epithelial mapping with OCT and vision quality was assessed with the optical quality analysis system using the objective scatter index (OSI). Central, superior, inferior, minimum, maximum, and standard deviation of epithelium thickness (Irregularity), were analysed and correlations with the OSI were calculated. Results The mean (±SD) central, inferior and maximum epithelial thicknesses of the EBMD patients (respectively, 56.4 (±8.1) μm, 58.9 (±6.4) μm, and 67.1 (±8.3) μm) were thicker compared to DED patients (P<0.05) and normal subjects (P<0.05). We found greater irregularity of epithelial thickness in EBMD (5.1±2.5 μm) compared to DED patients (2.6±1.0 μm) (P = 4.4.10−6) and normal subjects (2.1±0.7 μm) (P = 7.6.10−7). The mean OSI was worse in EBMD patients than in DED patients (P = 0.01) and compared to normal subjects (P = 0.02). The OSI correlated with the epithelial thickness irregularity (Spearman coefficient = 0.54; P = 2.65.10−5). Conclusions The OCT pachymetry map demonstrated that EBMD patients had thicker corneal epithelium in the central and inferior region. These changes were correlated with objective measurements of vision quality. This OCT characterisation of the EMBD provides a better understanding of the epithelial behaviour in this dystrophy and its role in vision quality.
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Smith, Timothy D., Hayley M. Corbin, Scot E. E. King, Kunwar P. Bhatnagar, and Valerie B. DeLeon. "A comparison of diceCT and histology for determination of nasal epithelial type." PeerJ 9 (November 3, 2021): e12261. http://dx.doi.org/10.7717/peerj.12261.
Повний текст джерелаАнотація:
Diffusible iodine-based contrast-enhanced computed tomography (diceCT) has emerged as a viable tool for discriminating soft tissues in serial CT slices, which can then be used for three-dimensional analysis. This technique has some potential to supplant histology as a tool for identification of body tissues. Here, we studied the head of an adult fruit bat (Cynopterus sphinx) and a late fetal vampire bat (Desmodus rotundus) using diceCT and µCT. Subsequently, we decalcified, serially sectioned and stained the same heads. The two CT volumes were rotated so that the sectional plane of the slice series closely matched that of histological sections, yielding the ideal opportunity to relate CT observations to corresponding histology. Olfactory epithelium is typically thicker, on average, than respiratory epithelium in both bats. Thus, one investigator (SK), blind to the histological sections, examined the diceCT slice series for both bats and annotated changes in thickness of epithelium on the first ethmoturbinal (ET I), the roof of the nasal fossa, and the nasal septum. A second trial was conducted with an added criterion: radioopacity of the lamina propria as an indicator of Bowman’s glands. Then, a second investigator (TS) annotated images of matching histological sections based on microscopic observation of epithelial type, and transferred these annotations to matching CT slices. Measurements of slices annotated according to changes in epithelial thickness alone closely track measurements of slices based on histologically-informed annotations; matching histological sections confirm blind annotations were effective based on epithelial thickness alone, except for a patch of unusually thick non-OE, mistaken for OE in one of the specimens. When characteristics of the lamina propria were added in the second trial, the blind annotations excluded the thick non-OE. Moreover, in the fetal bat the use of evidence for Bowman’s glands improved detection of olfactory mucosa, perhaps because the epithelium itself was thin enough at its margins to escape detection. We conclude that diceCT can by itself be highly effective in identifying distribution of OE, especially where observations are confirmed by histology from at least one specimen of the species. Our findings also establish that iodine staining, followed by stain removal, does not interfere with subsequent histological staining of the same specimen.
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Bazan-Socha, Stanislawa, Sylwia Buregwa-Czuma, Bogdan Jakiela, Lech Zareba, Izabela Zawlik, Aleksander Myszka, Jerzy Soja, et al. "Reticular Basement Membrane Thickness Is Associated with Growth- and Fibrosis-Promoting Airway Transcriptome Profile-Study in Asthma Patients." International Journal of Molecular Sciences 22, no. 3 (January 20, 2021): 998. http://dx.doi.org/10.3390/ijms22030998.
Повний текст джерелаАнотація:
Airway remodeling in asthma is characterized by reticular basement membrane (RBM) thickening, likely related to epithelial structural and functional changes. Gene expression profiling of the airway epithelium might identify genes involved in bronchial structural alterations. We analyzed bronchial wall geometry (computed tomography (CT)), RBM thickness (histology), and the bronchial epithelium transcriptome profile (gene expression array) in moderate to severe persistent (n = 21) vs. no persistent (n = 19) airflow limitation asthmatics. RBM thickness was similar in the two studied subgroups. Among the genes associated with increased RBM thickness, the most essential were those engaged in cell activation, proliferation, and growth (e.g., CDK20, TACC2, ORC5, and NEK5) and inhibiting apoptosis (e.g., higher mRNA expression of RFN34, BIRC3, NAA16, and lower of RNF13, MRPL37, CACNA1G). Additionally, RBM thickness correlated with the expression of genes encoding extracellular matrix (ECM) components (LAMA3, USH2A), involved in ECM remodeling (LTBP1), neovascularization (FGD5, HPRT1), nerve functioning (TPH1, PCDHGC4), oxidative stress adaptation (RIT1, HSP90AB1), epigenetic modifications (OLMALINC, DNMT3A), and the innate immune response (STAP1, OAS2). Cluster analysis revealed that genes linked with RBM thickness were also related to thicker bronchial walls in CT. Our study suggests that the pro-fibrotic profile in the airway epithelial cell transcriptome is associated with a thicker RBM, and thus, may contribute to asthma airway remodeling.
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Qu, Dongyi, and Yuehua Zhou. "Post-Ortho-K Corneal Epithelium Changes in Myopic Eyes." Disease Markers 2022 (May 29, 2022): 1–5. http://dx.doi.org/10.1155/2022/3361172.
Повний текст джерелаАнотація:
The study is aimed at evaluating corneal epithelial thickness changes associated with overnight orthokeratology (ortho-K). In this retrospective study, epithelial thickness was measured using optical coherence tomography (OCT) before and after 1 day, 1 week, 1 month, and 3 months ortho-K nightly lens wear. Compared with pre-orthokeratology measurements, central (2 mm) corneal epithelium thickness was significantly reduced at 1 day, 1 week, 1 month, and 3 months with ortho-K ( P < 0.05 ). Paracentral (2 mm~5 mm annular ring) epithelial thickness was also significantly reduced at superior temporal, inferior temporal, temporal, and inferior locations after ortho-K ( P < 0.05 ), while midperipheral (5 mm~6 mm annular ring) epithelial thickness was greater post- than pre-ortho-K at superior, superior temporal, inferior temporal, inferior, and inferior nasal locations ( P < 0.05 ). In other zones, superior, superior nasal, nasal, and inferior nasal in paracentral annular ring and temporal and superior nasal in midperipheral ring, epithelial thickness underwent no significant change. Ortho-K lens wear caused the central corneal epitheliums to thin. The temporal half zones become thinner in paracentral zones and thicker in midperipheral zones.
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Sun, Yan, Amy G. Hise, Carolyn M. Kalsow, and Eric Pearlman. "Staphylococcus aureus-Induced Corneal Inflammation Is Dependent on Toll-Like Receptor 2 and Myeloid Differentiation Factor 88." Infection and Immunity 74, no. 9 (September 2006): 5325–32. http://dx.doi.org/10.1128/iai.00645-06.
Повний текст джерелаАнотація:
ABSTRACT Toll-like receptors (TLRs) expressed by the corneal epithelium represent a first line of host defense to microbial keratitis. The current study examined the role of TLR2, TLR4, and TLR9 and the common adaptor molecule myeloid differentiation factor 88 (MyD88) in a Staphylococcus aureus model of corneal inflammation. The corneal epithelia of C57BL/6, TLR2−/−, TLR4−/−, TLR9−/−, and MyD88−/− mice were abraded using a trephine and epithelial brush and were exposed to heat- or UV-inactivated S. aureus clinical strain 8325-4 and other clinical isolates. Corneal thickness and haze were measured by in vivo confocal microscopy, neutrophil recruitment to the corneal stroma was quantified by immunohistochemistry, and cytokine production was measured by enzyme-linked immunosorbent assay. The exposure of corneal epithelium to S. aureus induced neutrophil recruitment to the corneal stroma and increased corneal thickness and haze in control C57BL/6 mice but not in TLR2−/− or MyD88−/− mice. The responses of TLR4−/− and TLR9−/− mice were similar to those of C57BL/6 mice. S. aureus-induced cytokine production by corneal epithelial cells and neutrophils was also significantly reduced in TLR2−/− mice compared with that in C57BL/6 mice. These findings indicate that S. aureus-induced corneal inflammation is mediated by TLR2 and MyD88 in resident epithelial cells and infiltrating neutrophils.
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Vanoni, Simone, Giada Scantamburlo, Silvia Dossena, Markus Paulmichl, and Charity Nofziger. "Interleukin-Mediated Pendrin Transcriptional Regulation in Airway and Esophageal Epithelia." International Journal of Molecular Sciences 20, no. 3 (February 9, 2019): 731. http://dx.doi.org/10.3390/ijms20030731.
Повний текст джерелаАнотація:
Pendrin (SLC26A4), a Cl−/anion exchanger, is expressed at high levels in kidney, thyroid, and inner ear epithelia, where it has an essential role in bicarbonate secretion/chloride reabsorption, iodide accumulation, and endolymph ion balance, respectively. Pendrin is expressed at lower levels in other tissues, such as airways and esophageal epithelia, where it is transcriptionally regulated by the inflammatory cytokines interleukin (IL)-4 and IL-13 through a signal transducer and activator of transcription 6 (STAT6)-mediated pathway. In the airway epithelium, increased pendrin expression during inflammatory diseases leads to imbalances in airway surface liquid thickness and mucin release, while, in the esophageal epithelium, dysregulated pendrin expression is supposed to impact the intracellular pH regulation system. In this review, we discuss some of the recent findings on interleukin-mediated transcriptional regulation of pendrin and how this dysregulation impacts airway and esophagus epithelial homeostasis during inflammatory diseases.
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Mustonen, MV, MH Poutanen, S. Kellokumpu, Y. de Launoit, VV Isomaa, RK Vihko, and PT Vihko. "Mouse 17 beta-hydroxysteroid dehydrogenase type 2 mRNA is predominantly expressed in hepatocytes and in surface epithelial cells of the gastrointestinal and urinary tracts." Journal of Molecular Endocrinology 20, no. 1 (February 1, 1998): 67–74. http://dx.doi.org/10.1677/jme.0.0200067.
Повний текст джерелаАнотація:
17 beta-Hydroxysteroid dehydrogenase (17HSD) type 2 efficiently catalyzes the conversion of the high activity 17 beta-hydroxy forms of sex steroids into less potent 17-ketosteroids. In the present study in situ hybridization was utilized to analyze the cellular localization of 17HSD type 2 expression in adult male and female mice. The data indicate that 17HSD type 2 mRNA is expressed in several epithelial cell layers, including both absorptive and secretory epithelia as well as protective epithelium. In both males and females, strong expression of 17HSD type 2 was particularly detected in epithelial cells of the gastrointestinal and urinary tracts. The mRNA was expressed in the stratified squamous epithelium of the esophagus, and surface epithelial cells of the stomach, small intestine and colon. The hepatocytes of the liver and the thick limbs of the loops of Henle in the kidneys, as well as the epithelium of the urinary bladder, also showed strong expression of 17HSD type 2 mRNA in both male and female mice. In the genital tracts, low 17HSD type 2 expression was detected in the seminiferous tubules, the uterine epithelial cells and the surface epithelium of the ovary. Expression of the mRNA was also detected in the sebaceous glands of the skin. The results indicate that in both male and female mice, 17HSD type 2 is expressed mainly in the various epithelial cell types of the gastrointestinal and urinary tracts, and therefore suggest a role for the enzyme in steroid inactivation in a range of tissues and cell types not considered as classical sex steroid target tissues.
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Martyniuk, Kamila, Natalia Ziółkowska, Maria Hanuszewska-Dominiak, Natalia Szyryńska, and Bogdan Lewczuk. "Histology and Ultrastructure of the Esophagus in European Beaver (Castor fiber) Displays Features Adapted to Seasonal Changes in Diet." Animals 13, no. 4 (February 11, 2023): 635. http://dx.doi.org/10.3390/ani13040635.
Повний текст джерелаАнотація:
The European beaver is a herbivorous rodent whose diet changes seasonally, and in winter consists of large quantities of woody plants. It is distinguished among other mammals by a unique organization of the stomach that comprises the cardiogastric gland and by the unusual process of mucus formation in the gastric mucosa. The aim of study was to (i) characterize the structure of the beaver esophagus with particular attention to the mucosal epithelium; (ii) compare the histological structure of the esophagi collected in spring, summer, and winter; (iii) provide preliminary data on the structure of the esophagus in beaver fetuses. The study was conducted on esophagi of 18 adult beavers captured in Poland in April, August, and December, and on 3 fetal organs. The results obtained in adults show that the mucosa is lined with thick stratified squamous keratinized epithelium with a structure similar to that of the skin epidermis. Ultrastructural studies reveal the presence of multiple lamellar and non-lamellar bodies in granular cells, whose morphology and location gradually change while reaching the upper epithelial layers. The muscularis mucosa comprises a layer of longitudinally oriented bundles of smooth muscle cells. Both mucosa and submucosa do not comprise any glands. The thick muscularis externa consists mainly of internal circular and external longitudinal layers of striated muscle fibers. The keratinized layer of mucosa epithelium was 2-3-fold thicker in esophagi collected in winter than in those collected in spring and summer, while the epithelial cell layer thickness remained unchanged regardless of the season. Immunolabeling for proliferating cell nuclear antigen shows a higher index of epithelium proliferation in esophagi collected in winter than in spring and summer. No seasonal differences were noted in other layers of the esophagus. Fetal organs have epithelium covered with a keratinized layer, thinner than in adults, and the muscularis externa comprises both striated and smooth muscle cells.
Дисертації з теми "Epithelial thickne"
1
Kinikoglu, Beste F. "Tissue Engineering Of Full-thickness Human Oral Mucosa." Phd thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/12612770/index.pdf.
Повний текст джерелаАнотація:
Tissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal human oral mucosa biopsies in a collagen-glycosaminoglycan-chitosan scaffold, developed in our laboratory to construct a skin equivalent. An oral mucosal
equivalent closely mimicking the native one was obtained and characterized by histology, immunohistochemistry and transmission electron microscopy. Using the same model, the influence of mesenchymal cells on oral epithelial development was investigated by culturing epithelial cells on lamina propria, corneal stroma and dermal equivalents. They were found to significantly influence the thickness and the ultrastructure of the epithelium. Finally, in order to improve the adhesiveness of conventional scaffolds, an elastin-like recombinamer (ELR) containing the cell adhesion tripeptide, RGD, was used in the production of novel bilayer scaffolds employing lyophilization and electrospinning. These scaffolds were characterized by
mercury porosimetry, scanning electron microscopy and mechanical testing. In vitro tests revealed positive contribution of ELR on the proliferation of both fibroblasts and epithelial cells. It was thus possible to construct a viable oral mucosa equivalent using the principles of tissue engineering.
2
Lu, Fenghe. "BIOMECHANICAL ALTERATION OF CORNEAL MORPHOLOGY AFTER CORNEAL REFRACTIVE THERAPY." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/2961.
Повний текст джерелаАнотація:
Purpose: Although orthokeratology (non-surgical corneal reshaping, Corneal Refractive Therapy, CRT®) has been used for almost a half century, contemporary CRT's outcomes and mechanisms still require investigation. A series of studies was designed to examine different aspects of non-surgical corneal reshaping for myopic and hyperopic corrections, including the efficacy and stability of this procedure, the effect of the lens material characteristics (Dk/t), and the corneal or superficial structural change (e. g. corneal/epithelial thickness) in corneal reshaping. Methods: In the CRT® for myopia (CRT1) study, 20 myopes wore CRT® lenses on one eye and control lenses on the contralateral eye (eye randomized) for one night while sleeping. Corneal topography and refractive error were measured the night prior to lens insertion, immediately after lens removal on the following morning and at 20 and 60 minutes and 3, 6 and 12 hours later. In the CRT® for hyperopia (CRTH) study, 20 ametropes wore CRT®H lenses on one eye for one night while sleeping, the contralateral eye (no lens wear) served as control (eye randomized). Corneal topography, aberrations and refractive error were measured the night prior to lens insertion, immediately after lens removal on the following morning and at 1 and 3, 6, 12 and 28 hours later. In the relatively long term (4 weeks) CRT® for myopia (CRT2) study, 23 myopes wore CRT® lenses overnight and removed their lenses on awakening. Visual Acuity (VA), subjective vision, refractive error, aberrations, and corneal topography were measured at baseline, immediately after lens removal on the first day and 14 hours later, and these measurements were repeated on days 4, 10, and 28. The treatment zone size was demarcated by the change in corneal curvature from negative to positive and vice versa, using tangential difference maps from the corneal topographer. In the study of effects of Dk/t on CRT® for myopia (CRTHDK), 20 myopic subjects were fit with Menicon Z (MZ) lenses (Dk/t=90. 6, Paragon CRT®) on one eye and an Equalens II (EII) CRT® lenses (Dk/t=47. 2) on the contralateral eye (eye randomized). Corneal topography, refractive error and aberrations were measured before lens insertion (baseline), and the following day after overnight lens wear, on lens removal and 1, 3, 6, 12 hours later. In the study of short term effects of CRT® for myopia and hyperopia (STOK), 20 ametropes wore CRT® and CRT®H lenses in a random order on one eye (randomly selected). The lenses were worn for 15, 30 and 60 minutes (randomly ordered, with each period taking place on a different day). Refractive error, aberrations, corneal topography, and corneal/epithelial thickness (using OCT) were measured before and after lens wear. The measurements were performed on the control eyes at 60 minutes only.
Results: In the CRT1 study, after one night of CRT® for myopia, the central cornea flattened and the mid-periphery steepened, and myopia reduced. In the CRTH study, after one night of CRT® for hyperopia, the central cornea steepened and the para-central region flattened, myopia was induced or hyperopia was reduced, all aberrations except for the astigmatism increased and signed spherical aberration (SA) shifted from positive to negative. In the CRT2 study, after 4 weeks of CRT® lens wear, in general, the treatment zones stabilized by day 10, vision improved, myopia diminished, total aberration and defocus decreased and higher order aberrations (HOAs) including coma and SA increased. The visual, optical and subjective parameters became stable by day 10. In the CRTHDK study, after one night of CRT® (MZ vs. EII) lens wear, the central corneal curvature and aberration were similar with a slight exception: The mid-peripheral corneal steepening was greater in the EII (lower Dk/t) lens-wearing eyes compared to the MZ (higher Dk/t) eyes. In the STOK study, after brief CRT® and CRT®H lens wear, significant changes occurred from the 15 minutes time point: The corneal shape and optical performance changed in a predictable way; the central cornea swelled less than the mid-periphery after CRT® lens wear, whereas the central cornea swelled more than the para-central region after CRT®H lens wear; the central epithelium was thinner than the mid-periphery after CRT® lens wear and was thicker than the para-central region after CRT®H lens wear.
Conclusion: After one night of lens wear, CRT® and CRTH® lenses were effective for myopia and hyperopia correction, respectively. In the 4 week CRT study, the treatment zone size changed during the first 10 days. Its size was associated with VA, refractive error, aberrations, and subjective vision. In the CRTHDK study, after one night of lens wear, changes in corneal shape were slightly different, with more mid-peripheral steepening in the lower Dk lens-wearing eyes compared to the higher Dk lens-wearing eyes. Changes in central corneal shape and optical performance were similar in both eyes. In the STOK study, CRT® lenses for myopia and hyperopia induced significant structural and optical changes in as little as 15 minutes. The cornea, particularly the epithelium, is remarkably moldable, with very rapid steepening and flattening possible in a small amount of time.
3
Zwet, Marwa. "The extent of the role of apoptosis in oral lichen planus – a morphometric study." University of the Western Cape, 2016. http://hdl.handle.net/11394/5491.
Повний текст джерелаАнотація:
Magister Chirurgiae Dentium (MChD)Oral lichen planus (OLP) is a T-cell mediated chronic inflammatory disease with different clinical types that remains inscrutable in respect of its pathogenetic mechanisms and effective therapy. Increased apoptosis may influence the histopathological criteria of oral lichen planus (decrease in thickness of the epithelium and band of inflammatory infiltrate). Null hypothesis: The apoptotic rate does not correlate with a decrease in the epithelial thickness as well as the thickness of the band of inflammatory infiltrate in OLP. Aim: The present study aims to quantify apoptotic activity and to correlate the apoptotic rate with epithelial thickness as well as thickness of the inflammatory infiltrate of OLP cases diagnosed at Tygerberg Hospital from 2006 – 2015. Further, the epithelial thickness and thickness of the inflammatory infiltrate were also assessed for their association, if any. Materials and Methods: The study sample comprised 17 diagnostically verified cases of OLP. Sections stained with Haematoxylin and Eosin (H&E) were used to identify and count the number of apoptotic cells as well as measure the thickness of epithelium and the thickness of the lymphocytic inflammatory infiltrate by using software morphometric analysis (Zen Blue lite 2012). Statistical analysis was applied to analyse the correlation between apoptotic cells and histopathological features of OLP. Results: The present study's results showed no statistically significant association between the apoptotic rate, the epithelial thickness and the thickness of the lymphocytic inflammatory infiltrate.
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Ildgruben, Anna. "Human vaginal epithelial immunity and influences of hormonal contraceptive usage." Doctoral thesis, Umeå : Klinisk mikrobiologi, enh. för Immunologi och Klin. vetenskap, obstetrik och gynekologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-595.
Повний текст джерела
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Kinikoglu, Fatma Beste. "Tissue engineering of full-thickness human oral mucosa." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10310.
Повний текст джерелаАнотація:
L’ingénierie de la muqueuse orale humaine (MOH) a pour but le comblement des pertes de substances suite à un traumatisme facial ou à la chirurgie des lésions malignes. Elle a aussi des applications en recherche pour élucider les mécanismes biologiques de la MO et en pharmacotoxicologie comme alternative à l’expérimentation animale. L'objectif de cette thèse était de reconstruire une MOH proche du tissu normal. À cette fin, la faisabilité du concept a d'abord été testée par co-culture de fibroblastes de la lamina propria et de cellules épithéliales de MOH dans le substrat de collagène-chitosan glycosaminoglycane, développé pour la production de peaux reconstruites. La caractérisation de la MOH reconstruite par histologie, immunohistochimie et microscopie électronique à transmission a montré la présence d’une LP équivalente avec un épithélium pluristratifié et non kératinisé très proche du tissu d’origine. Grâce à ce modèle, nous avons ensuite démontré que l’origine des fibroblastes (MO, cornée, peau) influence significativement l’épaisseur et l’ultrastructure de l'épithélium obtenu par culture de cellules épithéliales orales. Enfin, afin d'améliorer les propriétés adhésives du substrat à base collagène, nous avons ajouté au collagène, une élastine-like recombinante (ELR) contenant le tri-peptide d’adhésion cellulaire, RGD, et produit un nouveau substrat bicouche, poreux par lyophilisation et recouvert d’une couche fibreuse par électrofilage. Ces substrats ont été caractérisés par porosimétrie au mercure, microscopie électronique à balayage et essais mécaniques. Nous avons démontré l’effet stimulant de ELR sur la prolifération des fibroblastes et des cellules épithélialesTissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal human oral mucosa biopsies in a collagen-glycosaminoglycan-chitosan scaffold, developed in our laboratory to construct a skin equivalent. An oral mucosal equivalent closely mimicking the native one was obtained and characterized by histology, immunohistochemistry and transmission electron microscopy. Using the same model, the influence of mesenchymal cells on oral epithelial development was investigated by culturing epithelial cells on lamina propria, corneal stroma and dermal equivalents. They were found to significantly influence the thickness and the ultrastructure of the epithelium. Finally, in order to improve the adhesiveness of conventional scaffolds, an elastin-like recombinamer (ELR) containing the cell adhesion tripeptide, RGD, was used in the production of novel bilayer scaffolds employing lyophilization and electrospinning. These scaffolds were characterized by mercury porosimetry, scanning electron microscopy and mechanical testing. In vitro tests revealed positive contribution of ELR on the proliferation of both fibroblasts and epithelial cells. It was thus possible to construct a viable oral mucosa equivalent using the principles of tissue engineering
6
Hutton, Christopher G. "Comparison of two different surgical approaches to increase peri-implant mucosa thickness: a randomized controlled clinical trial." Thesis, University of Iowa, 2016. https://ir.uiowa.edu/etd/2091.
Повний текст джерелаАнотація:
Objectives: Tooth replacement therapy using endosseous implants has become an essential component of contemporary dental practice. While a plethora of factors determine clinical success, the bucco-lingual and apico-coronal dimensions of the peri-implant mucosa play an important role in both esthetics and the maintenance of peri-implant health. Studies, most of which treat mucogingival defects in the natural dentition, comparing acellular dermal matrix (ADM) and autologous subepithelial connective tissue grafts (sCTG) have shown similar clinical outcomes. The purpose of this non-inferiority trial is to determine the clinical efficacy of ADM in the augmentation of peri-implant mucosa thickness (PMT) as compared to an autologous sCTG in human adults.
Methods: Twenty healthy adults treatment planned for a single tooth implant restoration in need of simultaneous peri-implant mucosa augmentation at the time of implant placement were recruited on the basis of an eligibility criteria. Patients were randomly assigned to the control group (autologous sCTG), or the experimental group (ADM allograft). Clinical measurements of mucosal thickness at the site were made with a periodontal probe and an endodontic spreader at baseline and 16 weeks post-op. These measurements were made by a masked, calibrated examiner. Gingival health, oral hygiene, wound healing and patient reported outcomes were also obtained. Mann-Whitney U tests were used to compare the mean mucosal thickness changes between the groups.
Results: The mean gain in PMT was approximately 1.5mm in the control group and 0.8mm in the experimental group. When measured at 1, 3 and 5mm apical from the CEJ, only the 3mm site exhibited a difference between the groups that approached statistical significance (control: 2.08 ± 0.80mm, test: 0.83 ± 1.37mm, Mann Whitney U = 10.00, p=0.05). Changes in keratinized mucosa width, healing index and patient reported outcomes were generally similar between the two groups.
Conclusions: Within the limitations of this study, both autologous sCTG and ADM appear to be adequate materials to augment PMT without sacrificing other relevant clinical parameters and/or patient related outcomes.
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Dai, Long-jun. "Control of intracelluar Ca²⁺ in epithelial cells of the kidney thick ascending limb." Thesis, 1995. http://hdl.handle.net/2429/7260.
Повний текст джерелаАнотація:
The cortical thick ascending limb of Henle’s loop (cTAL) plays a fundamental role in
salt reabsorption and concentration-dilution processes within the nephron. This study was
performed to characterize the role of intracellular free Ca²⁺ ([Ca²⁺]i) in hormone-mediated
signal transduction and to describe the function of Na⁺/Ca²⁺ exchange in control of [Ca²⁺]i
in isolated cTAL cells from porcine kidney. Parathyroid hormone, arginine vasopressin, and
atrial natriuretic peptide transiently increased [Ca²⁺]i, in a dose-dependent manner. The
increment in [Ca²⁺]i induced by these hormones was by intracellular release and entry
through plasma membrane Ca²⁺ channels. These hormone-induced Ca²⁺ transients were
modulated by cAMP, cGMP, and PKC activation. In order for intracellular Ca²⁺ to play
a role in signal transduction mechanisms it is necessary to have regulated processes which
maintain [Ca²⁺]i at submicromolar levels. We evaluated the functional role of Na⁺/Ca²⁺
exchange in cTAL cells. cTAL cells treated with ouabain had basal [Ca²⁺]i 86±2 nM.
Removal of external Na⁺ or voltage depolarization with KC1 resulted in rapid and
reversible maximal elevation of [Ca²⁺]i 1023 ±72 nM (n=28), which was dependent on the
presence of external Ca²⁺ and elevated [Na⁺]i. The activity of Na⁺/ Ca²⁺ exchange was
modulated by protein phosphorylation as calmodulin inhibition decreased and phosphatase
inhibition increased the apparent exchange activity. The presence of a Na⁺/ Ca²⁺ exchanger
was confirmed with northern hybridization techniques. A gene transcript which encodes
a portion of the intracellular ioop of the renal Na⁺/ Ca²⁺ exchanger was amplified from
cortical tissue and cTAL cells by polymerase chain reaction (PCR) using primers flanking
the alternative splicing site. Southern hybridization and DNA sequencing demonstrated
the isoform contained exons B and D characteristic of one isoform (NACA3) of the renal Na⁺/ Ca²⁺ exchanger. The results provide both functional and molecular evidence for a
N2aICa exchanger in cTAL cells of the porcine kidney. It is likely that Na⁺/ Ca²⁺
exchange plays an important role in [Ca²⁺]i control and thus hormonal regulation of
electrolyte reabsorption within the cTAL cells.
Книги з теми "Epithelial thickne"
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Lennon, Rachel, and Neil Turner. The molecular basis of glomerular basement membrane disorders. Edited by Neil Turner. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199592548.003.0320_update_001.
Повний текст джерелаАнотація:
The glomerular basement membrane (GBM) is a condensed network of extracellular matrix molecules which provides a scaffold and niche to support the function of the overlying glomerular cells. Within the glomerulus, the GBM separates the fenestrated endothelial cells, which line capillary walls from the epithelial cells or podocytes, which cover the outer aspect of the capillaries. In common with basement membranes throughout the body, the GBM contains core components including collagen IV, laminins, nidogens, and heparan sulphate proteoglycans. However, specific isoforms of these proteins are required to maintain the integrity of the glomerular filtration barrier.Across the spectrum of glomerular disease there is alteration in glomerular extracellular matrix (ECM) and a number of histological patterns are recognized. The GBM can be thickened, expanded, split, and irregular; the mesangial matrix may be expanded and glomerulosclerosis represents a widespread accumulation of ECM proteins associated with loss of glomerular function. Whilst histological patterns may follow a sequence or provide diagnostic clues, there remains limited understanding about the mechanisms of ECM regulation and how this tight control is lost in glomerular disease. Monogenic disorders of the GBM including Alport and Pierson syndromes have highlighted the importance of both collagen IV and laminin isoforms and these observations provide important insights into mechanisms of glomerular disease.
Частини книг з теми "Epithelial thickne"
1
de Toledo, Márcia Cristina, and Bruno Viana Gonçalves. "Corneal Epithelial Thickness Mapping in Keratoconus." In Keratoconus, 979–87. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-85361-7_85.
Повний текст джерела
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Reinstein, Dan Z., Timothy J. Archer, Marine Gobbe, Raksha Urs, and Ronald H. Silverman. "Diagnosing Keratoconus Using VHF Digital Ultrasound Epithelial Thickness Profiles." In Keratoconus, 151–66. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-43881-8_13.
Повний текст джерела
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Gupta, Brij L. "1 μm Thick Frozen Hydrated/Dried Sections for Analysing Pericellular Environment in Transport Epithelia; New Results from Old Data." In Electron Probe Microanalysis, 199–212. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74477-8_15.
Повний текст джерела
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Greger, R., and E. Schlatter. "Electrolyte Activities in Cl−-Transporting Epithelia: Cortical Thick Ascending Limb of Rabbit Nephron and Rectal Gland Tubules of the Spiny Dogfish, Squalus acanthias." In Ion Measurements in Physiology and Medicine, 301–8. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70518-2_47.
Повний текст джерела
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Bueno, Juliana. "Cystic Lung Disease." In Chest Imaging, 429–33. Oxford University Press, 2019. http://dx.doi.org/10.1093/med/9780199858064.003.0074.
Повний текст джерелаАнотація:
A lung cyst is a round parenchymal lucency or low-attenuating area with a well-defined interface with adjacent normal lung and surrounded by an epithelial or fibrous wall that is often less than 2 mm in thickness. Diseases that manifest with lung cysts comprise a limited but well known group of entities. Classic diseases manifesting with diffuse lung cysts include pulmonary Langerhans cell histiocytosis (PLCH), lymphangioleiomyomatosis (LAM), lymphocytic interstitial pneumonia (LIP) and Birt-Hogg-Dubé syndrome. However, it is important to recognize other conditions that may mimic cystic lung disease, such as pneumatoceles, tracheobronchial papillomatosis, cavitary disease (e.g. tuberculosis, fungal infection), bronchiectasis, and emphysema. Differentiation between true cysts and cyst mimics may be difficult, and may rely on identification of ancillary findings.
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Atkinson, Martin E. "Skin and fascia." In Anatomy for Dental Students. Oxford University Press, 2013. http://dx.doi.org/10.1093/oso/9780199234462.003.0013.
Повний текст джерелаАнотація:
Skin is a specialized boundary tissue which forms the entire external surface of the body and is continuous with mucosa lining the respiratory, gastrointestinal, and urinogenital tracts at their respective openings. Skin is the largest organ in the body but is often overlooked in this respect. Skin has many functions, some of which are not immediately obvious. • It minimizes damage from mechanical, thermal, osmotic, chemical, and sunlight insults. • It forms a barrier against microorganisms. • It has a major function in thermoregulation. • It is a sensory surface equipped with touch, pressure, temperature, and pain receptors. • It has good frictional properties useful in locomotion and handling objects. • It is waterproof. • It is the site of vitamin D synthesis. • It also plays a role in non-verbal communication when we blush, alter our facial expression, or use tactile communication such as touching or kissing. Skin has two distinct parts when seen under a microscope, the superficial epidermis and the deeper dermis. The epidermis is a surface epithelium in which the outer cells are keratinized. Keratinization is the deposition of tough mats of keratin which are intracellular fibrous proteins that make the cells tough; keratinization also kills the superficial cells so the outer layers of your skin are dead. The epidermis varies in thickness. The thickest and most heavily keratinized areas are on the soles of the feet and palms of the hands whereas the epidermis on the face and back of the hand is much thinner and less heavily keratinized. Habitual activity, such as holding a pen, digging with a shovel or using scissors, may produce localized thickenings of thick skin by increasing the thickness of keratin to produce calluses. Cells below the keratin layer have a special coating that forms a permeability barrier, preventing water moving between cells, thus preventing water loss from the body and water-logging when exposed to water. Epithelium does not contain blood vessels, which is why you do not bleed when you lightly knock your skin. To bleed, you need to expose the blood vessels that lie in the dermis and supply the overlying epidermis by diffusion of nutrients through fenestrated capillaries.
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Reinstein, Dan, Timothy Archer, and Marine Gobbe. "Diagnosing Keratoconus Using Artemis VHF Digital Ultrasound Epithelial Thickness Profiles." In Textbook on Keratoconus: New Insights, 96. Jaypee Brothers Medical Publishers (P) Ltd., 2012. http://dx.doi.org/10.5005/jp/books/11483_12.
Повний текст джерела
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"Corneal Dystrophies." In Medical Atlas of Cornea and External Diseases in Middle Eastern Populations, 251–64. IGI Global, 2022. http://dx.doi.org/10.4018/978-1-7998-6937-5.ch008.
Повний текст джерелаАнотація:
Corneal dystrophies have classically referred to inherited, bilateral disease without systemic findings, although there are several exceptions to this definition. Hereditary pattern is not present in most patients with epithelial basement membrane dystrophy (EBMD). Unilateral corneal changes may be found in some patients with posterior polymorphous corneal dystrophy (PPCD). TGFBI gene mutation (p.His572del) is associated with a unilateral, late-onset variant of lattice corneal dystrophy. Among all dystrophies, macular corneal dystrophy and posterior amorphous corneal dystrophy are associated with decreased corneal thickness. The International Committee for Classification of Corneal Dystrophies (IC3D) was created in 2005 to revise the corneal dystrophy nomenclature and create a current and accurate corneal dystrophy classification system. Evidential categories were created in the IC3D classification for reflecting the natural evolution of a corneal dystrophy and indicate the level of evidence supporting the existence of a given dystrophy.
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Kayal, Abraham. "The Physiology of Tear Film." In Dry Eye [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98945.
Повний текст джерелаАнотація:
The precorneal tear film is a thin layer, about 2–5.5 μm thick, which overlays the corneal and conjunctival epithelium. It functions to lubricate and protect the corneal and eyelid interface from environmental and immunological factors as well as provide an optical medium. The tear film is depicted as a three-layered structure: lipid, aqueous, and mucous layers. Within each layer possesses a different composition which dictates its function. In common between the three layers are their homeostatic process of evaporation and drainage. Any dysfunction in either of the layers can result in Dry Eye Syndrome (DES). The composition, regulation, and pathology of tear film will be discussed in this chapter.
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Reinstein, Dan Z., Timothy J. Archer, and Ryan S. Vida. "Epithelial Thickness Mapping for Keratoconus Screening by VHF Digital Ultrasound or Anterior Segment OCT." In Keratoconus, 257–78. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-75978-6.00018-2.
Повний текст джерелаТези доповідей конференцій з теми "Epithelial thickne"
1
Molladavoodi, Sara, John B. Medley, Maud Gorbet, and H. J. Kwon. "Mechanotransduction in Corneal Epithelial Cells." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65406.
Повний текст джерелаАнотація:
Mechanical properties of the cornea can be affected by diseases such as keratoconus. In keratoconus, a decrease in both thickness and rigidity of the cornea is observed. It is currently not clear whether and how changes in mechanical properties of the cornea are associated with corneal epithelial cell behavior. In the present study, polyacrylamide (PAA) gels with different elastic moduli have been prepared and human corneal epithelial cells (HCECs) have been cultured on them. To investigate the effect that changes in elastic modulus may have on adhesion and migration of corneal epithelial cells, actin filament organization and expression of adhesion molecules were characterized. It was found that HCECs actin filament organization improves with increasing substrate stiffness and integrin α3 expression significantly increases on more compliant substrates.
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Akiba, M., A. Kubota, C. Reisman, Y. Fukuma, K. Nishida, and K. P. Chan. "Evaluation of cultured corneal epithelial cells and epithelial thickness by full-field optical coherence tomography." In Biomedical Optics. Washington, D.C.: OSA, 2008. http://dx.doi.org/10.1364/biomed.2008.bmd71.
Повний текст джерела
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Taber, Larry A. "Mechanical Model for Myocardial Trabeculation." In ASME 1998 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1998. http://dx.doi.org/10.1115/imece1998-0193.
Повний текст джерелаАнотація:
Abstract Trabeculation of the embryonic heart is studied using a biomechanical model for epithelial morphogenesis. The analysis of the model includes large deformation and cytoskeletal activation. Illustrative results for a membrane on an elastic foundation (substrate) illustrate the pattern-forming capability of the model, with concentrations in membrane thickness representing trabeculae.
4
Nerurkar, Nandan L., and Cliff J. Tabin. "Collective Cell Movements Drive Morphogenesis and Elongation of the Avian Hindgut." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14438.
Повний текст джерелаАнотація:
At the end of gastrulation, the endoderm forms a single-cell thick epithelium lining the ventral surface of the developing embryo. Subsequently, through a series of poorly understood events, the initially flat endoderm is transformed into the gut tube, a cylindrical structure that gives rise to the epithelial lining of the entire respiratory and gastrointestinal tracts. In birds and mammals, formation of the gut tube begins with two invaginations at the anterior (head) and posterior (tail) poles of the embryo, termed the anterior (AIP) and caudal intestinal portals (CIP). It is thought that the AIP and CIP begin moving toward one another as two progressing waves of lateral-to medial folding (from left and right toward center), “zipping” the gut tube closed along the embryonic midline (Fig. 1A). This view of lateral-to-medial folding is, however, inconsistent with several observations. For example, fate mapping studies in chick and mouse that suggest that cells originating in the posterior end (toward the tail) of the flat endoderm do not form the hindgut, but instead contribute to the more anterior midgut [1, 2]. This would not be possible in a simple lateral-to-medial folding process. Therefore, it is largely unknown how this fundamental structure of the vertebrate body plan is established. The objective of the present work is to apply multi-photon live imaging of the chick embryo to determine how the hindgut is formed. Our findings suggest the hindgut arises from directed, collective cell movements that drive antero-posterior folding of the initially flat endoderm.
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Tai, Cheng-feng, David Halpern, and James B. Grotberg. "Airway Closure With Two Liquid Layers." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53504.
Повний текст джерелаАнотація:
There are several mechanisms which may cause airway closure in the lung. In this paper, we focus on airway closure due to the capillary instability [1]. In Gauglitz and Radke’s study [2], they showed that once the ratio of film thickness to tube radius is larger than 0.12, airway closure could occur. The induced interfacial deformation creates a driving pressure which forces more liquid into a growing bulge. The interface will then deform rapidly towards the end of the closure process due to the presence of large curvatures, which create strong driving pressures and will eventually lead to the formation of a liquid plug. Due to the instability, stresses, including normal and shear stresses on the airway wall, will be induced. The epithelial cells on the inner wall of the airway may be injured by these induced stresses. The purpose of this study is to calculate the wall stresses for a two-layer system and determine if the magnitude of the stresses are sufficient to injure the epithelial cells.
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Tai, Cheng-feng, David Halpern, and James B. Grotberg. "Two Layer Fluid Stress Analysis During Airway Closure." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19375.
Повний текст джерелаАнотація:
There are several mechanisms which may cause airway closure in the lung. In this paper, we focus on airway closure due to the capillary instability [1]. In Gauglitz and Radke’s study [2], they showed that once the ratio of film thickness to tube radius is larger than 0.12, airway closure could occur. The induced interfacial deformation creates a driving pressure which forces more liquid into a growing bulge. The interface will then deform rapidly towards the end of the closure process due to the presence of large curvatures, which create strong driving pressures and will eventually lead to the formation of a liquid plug. Due to the velocity and pressure gradients in the liquid film caused by the instability, stresses, including normal and shear stresses on the airway wall, will be induced. The epithelial cells on the inner wall of the airway may be injured by these induced stresses. The purpose of this study is to measure the stresses on airway numerically and determine if the magnitude of the stresses are sufficient to injure the epithelial cells.
7
Hattery, David W., Brenda Hattery, Moinuddin Hassan, Victor V. Chernomordik, Abby Vogel, Farid Hekmat, and Amir H. Gandjbakhche. "Optical quantification of epithelial layer thickness as a measure of oral inflammation." In Biomedical Optics 2003, edited by Peter Rechmann, Daniel Fried, and Thomas Hennig. SPIE, 2003. http://dx.doi.org/10.1117/12.476656.
Повний текст джерела
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Jetta, Deekshitha, Tasnim Shireen, Rajath D. Prabhu, and Susan Z. Hua. "Contribution of Piezo1 in ECM Stiffness Incited Epithelial Cell Remodeling." In ASME 2022 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/imece2022-95032.
Повний текст джерелаАнотація:
Abstract Cells are sensitive to mechanical cues from the ECM such as, stiffness, topography, and roughness. Tissue stiffening due to ageing or diseases has been shown to affect cell proliferation, differentiation, migration, and apoptosis, but the mechanosensors that respond to ECM stiffness changes have not yet been fully understood. Recent studies on mechanosensitive Piezo1 channels have shown that these channels are sensitive to ECM cues, such as mechanical confinements implemented by micropatterns. We hypothesize that Piezo1 could serve as a force sensor for local ECM stiffness. Using a ‘2-well PDMS chip’ consisting of substrates of different stiffness, namely, hard (∼1000 kPa) and soft (∼0.1 kPa), we studied the role of Piezo1 on stiffness-dependent morphology changes in epithelial cells. The results show that cells respond to substrate stiffness variations with profound cytoskeletal reorganization and moderate changes in their spreading area and shape. Cells on the hard substrates were only ∼20% larger than that on soft substrates within the same culture period of 2.5 hrs and at a similar confluency. However, cells on hard substrates show abundant F-actin bundles that are reorganized to peripheral actin rings on soft substrates. Inhibition of Piezo1 with GsMTx4 or Gd3+, largely reduced the formation of thick actin bundles on hard substrates. Activation of Piezo1 with specific agonist, Yoda1, enhanced the formation of actin bundles. These results indicate that the epithelial cells’ response to ECM stiffness is mediated by Piezo1 through its function of permeating Ca2+ ions. This study demonstrates that Piezo1 could be an ECM mechanosensor in epithelial cells.
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Li, Yan, Ou Tan, and David Huang. "Normal and keratoconic corneal epithelial thickness mapping using Fourier-domain optical coherence tomography." In SPIE Medical Imaging, edited by John B. Weaver and Robert C. Molthen. SPIE, 2011. http://dx.doi.org/10.1117/12.878567.
Повний текст джерела
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Prestin, S., C. Betz, and M. Kraft. "Measurement of epithelial thickness within the oral cavity using optical coherence tomography (OCT)." In BiOS, edited by Nikiforos Kollias, Bernard Choi, Haishan Zeng, Reza S. Malek, Brian J. Wong, Justus F. R. Ilgner, Kenton W. Gregory, et al. SPIE, 2010. http://dx.doi.org/10.1117/12.853543.
Повний текст джерелаЗвіти організацій з теми "Epithelial thickne"
1
Jangir, Hemlata, Aparna Ningombam, Arulselvi Subramanian, and Subodh Kumar. Traumatic Jejunal Mesenteric Pseudocyst in the Vicinity of Blunt Abdominal Trauma with a Brief Review of Literature. Science Repository, January 2023. http://dx.doi.org/10.31487/j.ajscr.2022.04.04.
Повний текст джерелаАнотація:
Mesenteric pseudocyst (MP) is a rare heterogeneous group of intra-abdominal benign cystic lesions with different etiopathogenesis and clinically silent behaviours. These lesions are introduced as one of the entities based on the histological features of thick fibrous cyst walls, barren of the epithelial lining. Often, they present as expanding abdominal masses or are diagnosed incidentally in conventional radiological studies, exploratory laparotomies, or with symptoms of complications such as infection, torsion, or rupture. Surgical removal of the cyst, with or without resection of the affected intestinal segment, is the treatment of choice. Depending upon the size and location of the lesion and related complications, it can be managed by open surgical procedures or laparoscopic approach. Only a handful of 7 cases of traumatic mesenteric cysts have been reported yet in the vicinity of blunt abdominal trauma. We report a rare incidentally detected case of mesenteric pseudocyst (traumatic) in a male of early 20s with a history of blunt abdominal trauma 13 months back and for which serial abdominal exploratory laparotomies were performed. A brief review of the literature is provided, conforming to the rarity of the case. This case highlights the role of histomorphology in diagnosing a benign cystic entity with accuracy, that could be misdiagnosed as infectious granulomatous lesion.