Дисертації з теми "Enzymes Separation"
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Haileselassie, Seble Sereke Berhan. "Production of enzyme-modified cheese and bioactive peptides by Lactobacillus and commercial enzymes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50782.pdf.
Повний текст джерелаPerraud, Xavier. "Characterization of lipoxygenases and associated enzymes from selected microorganisms." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0032/NQ64642.pdf.
Повний текст джерелаWang, Yan. "Pretreatment and Enzymatic Treatment of Spruce : A functional designed wood components separation for a future biorefinery." Doctoral thesis, KTH, Träkemi och massateknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-150395.
Повний текст джерелаQC 20140903
Rodrigues, Eliana Maria Gonçalves. "Extração liquido-liquido de xilanase por micela reversa numa microcoluna de campanulas pulsadas." [s.n.], 2001. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267501.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica
Made available in DSpace on 2018-07-28T22:32:01Z (GMT). No. of bitstreams: 1 Rodrigues_ElianaMariaGoncalves_D.pdf: 3569293 bytes, checksum: b7c2f26dc727eabf16b3528d0a1813bb (MD5) Previous issue date: 2001
Resumo: Neste trabalho, foi estudada uma microcoluna agitada por campânulas pulsadas, visando promover um eficiente contato entre as fases através de uma agitação suave, aumentando assim o tempo de contato entre elas no interior da microcoluna e também evitando a desnaturação da enzima. O objetivo deste estudo visou a recuperação da xilanase, produzida pelo fungo Penicillium janthinellum, através da técnica de extração líquido - líquido por micela reversa, para o interior micelar. Para tanto, foi utilizado o agente tensoativo catiônico BDBAC (c1oreto de benzil dodecil bis (hidroxietil) amônio). Foram utilizados planejamentos estatísticos com o intuito de se realizar uma triagem das variáveis significativas no processo: freqüência de pulsação das campânulas, razão entre as fases aquosa/orgânica e condutividade da fase aquosa; a metodologia de superficie de resposta foi empregada para a quantificação dos níveis das mesmas. O trabalho resultou em dois modelos matemáticos, um que representa a recuperação da enzima pura, e outro que representa a recuperação da enzima presente no extrato enzimático bruto. Foram previstos e observados experimentalmente para ambos os modelos obtidos, rendimento em atividade na ordem de 140% para a enzima pura e 43% para o extrato enzimático. Verificou-se neste trabalho que, a metodologia estatística empregada foi de extrema importância e que, a microcoluna estudada tem operação estável e altos rendimentos em atividade foram alcançados
Abstract: In this work, it was studied a microcolumn agitated by puIsed caps, due to promote an efficient contact between the phases in the column and also to avoid the enzyme denaturation and the loss of main proteins properties. This work deals with the purification of xylanase produced by Penicillium janthinellum by liquid-liquid extraction on reverse micelles, to micellar inner, utilizing a cationic surfactant BDBAC (N-benzyI-N-dodecyl-N-bis(2-hydroxyethyl)). It was used design statistical with the intention of realized selection of the main variables in the process: frequency pulsed, volumetric flow and ionic strength; the response surface methodology was employed to the quantified levels of this. This resulted in two major mathematical models: one representing the use of pure enzyme and the other the use of enzymatic extract. It was predict and observed experimentally for both models, obtained activity yield in the order of 140% to the pure enzyme and 43% to the enzymatic extract. It was observed in this work that, the methodology statistical employed was extremely important and that the microcolumn used were stable operation and that high activity yield was reached.
Doutorado
Desenvolvimento de Processos Biotecnologicos
Doutor em Engenharia Química
Pigeon, Dominique. "Etude des enzymes de synthèse des catécholamines et phosphorylation de la tyrosine hydroxylase de phéochromocytome de rat." Paris 6, 1986. http://www.theses.fr/1986PA066168.
Повний текст джерелаSadler, Andrew Michael. "The separation and immobilisation of a yeast intracellular enzyme." Thesis, University of Surrey, 1988. http://epubs.surrey.ac.uk/847980/.
Повний текст джерелаOlceroglu, Ayse Hande. "Chiral Separations By Enzyme Enhanced Ultrafiltration: Fractionation Of Racemic Benzoin." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12607460/index.pdf.
Повний текст джерелаwater solvent. Effect of BAL concentration on total benzoin retention and ee% was investigated. It was found that
for all the studied BAL concentrations in the range of 650- 1936 ppm total benzoin retention and ee % were kept almost constant at ~75% and ~60%, respectively.
Prinz, Axel [Verfasser]. "Enzyme Separation Using Aqueous Two-Phase Extraction: Experiment, Model and Simulation / Axel Prinz." München : Verlag Dr. Hut, 2014. http://d-nb.info/1053859686/34.
Повний текст джерелаChen, Cheau-Yun. "Studies of Enzyme Mechanism Using Isotopic Probes." Thesis, North Texas State University, 1987. https://digital.library.unt.edu/ark:/67531/metadc331996/.
Повний текст джерелаChen, Zhiqiang. "NANOMETER-SCALE MEMBRANE ELECTRODE SYSTEMS FOR ACTIVE PROTEIN SEPARATION, ENZYME IMMOBILIZATION AND CELLULAR ELECTROPORATION." UKnowledge, 2014. http://uknowledge.uky.edu/cme_etds/33.
Повний текст джерелаKunze, Anna-Katharina [Verfasser]. "Intensified reactive absorption processes for CO2 separation using enzyme accelerated solvents / Anna-Katharina Kunze." München : Verlag Dr. Hut, 2016. http://d-nb.info/1084385406/34.
Повний текст джерелаRoth, Hans-Christian [Verfasser]. "Design of nanoscale enzyme carriers and their separation from highly viscous liquors / Hans-Christian Roth." München : Verlag Dr. Hut, 2017. http://d-nb.info/1135596964/34.
Повний текст джерелаYahia, Marei Abdelrahim Mohamed. "Bio(molecular) control of selective ion transport, gas separation and catalytic enzyme-based reactions using functionalized membranes." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS251/document.
Повний текст джерелаDifferent research works have been described in this thesis. The research works can be summarized as the following. The first chapter deals with the identification of effective potent inhibitors for the human carbonic anhydrase I (hCAI) isozyme. Considering the pharmacological importance to find selective CA inhibitors (CAIs) and CA activators (CAAs), human carbonic anhydrase I (hCAI) has been subjected to a parallel screening of various constitutional dynamic libraries (CDL). In the second chapter, constitutional dynamic networks have been used in liquid and solid membrane systems as a carrier network for transporting lanthanides. The transport is based on the complexing ability of lanthanides metals (La+3, Lu+3, and Eu+3) with the functional polyether groups in the membrane materials. In the third chapter, the proposed approach consists in using supported ionic liquid membranes (SILMs) comprising two different carbonic anhydrase enzymes, the thermo-resistant SspCA enzyme and the Bovine-CA enzyme, which catalyze the reaction of reversible conversion of CO2 to bicarbonate, enhancing the driving force for CO2 transport. Membrane stability, CO2 and N2 permeability and (CO2/N2) ideal selectivity were determined for the membranes developed. In the fourth chapter, the research work consists in the synthesis and characterization of dense polymeric membranes for gas separation application. The gas permeability measurements for the synthesized polymeric membranes showed that the permeability of CO2 is higher than other used gases (N2 and CH4). In the last chapter, two different methods of PVDF membrane functionalization with a phosphotriesterase (PTE) enzyme have been developed to construct biocatalytic membrane reactor (BMR) for bioconversion and selective separation of paraoxon substrate. The first method employs reversible dispersion of magnetic nanoparticle immobilized with PTE using an external magnetic field on the surface of native PVDF membrane. On the contrary, the second method comprises chemical grafting of the PTE enzyme, after surface modification of the native PVDF membrane (DAMP-GA-Enzyme). Both methods of enzyme immobilization showed good efficiency and sensitivity towards the bioconversion of paraoxon substrate at different conditions applied in a biocatalytic membrane reactor (BMR).In general, the concepts developed in this thesis research work will help bring new tracks on the way to the development of a polymeric membrane for selective ion and gas separation but also for selective catalytic reaction under bio(molecular) control
Pruksasri, Suwattana. "Production and separation of galacto-oligosaccharides from lactose by β-galactosidase immobilized on nanofiltration membranes". The Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1190137539.
Повний текст джерелаKavurt, Ulku Bade. "Enzyme Enhanced Ultrafiltration For The Resolution Of Racemic Mandelic Acid." Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613483/index.pdf.
Повний текст джерелаRaak, Norbert, Raffaele Andrea Abbate, Albena Lederer, Harald Rohm, and Doris Jaros. "Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-234862.
Повний текст джерелаLi, Hao, Deyi Zhu, Yanchun Li, Shan Cao, Changhua Jiang, and Tianping Yu. "Analysis of the Functional Components of Acid Protease and Investigation of Bating Mechanism of Wet-blue - 36." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34266.
Повний текст джерелаSilva, Mojica Ernesto. "Polymer-silica Hybrids for Separation of CO2 and Catalysis of Organic Reactions." University of Akron / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=akron1398439043.
Повний текст джерелаFriberg, Andrew S. "Standardization of Islet Isolation and Transplantation Variables." Doctoral thesis, Uppsala universitet, Klinisk immunologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-150247.
Повний текст джерелаMémet, Sylvie. "Clonage des genes de structure des arn polymerases de la levure saccharomyces cerevisae et etude des genes de leurs plus grandes sous-unites." Paris 6, 1987. http://www.theses.fr/1987PA066186.
Повний текст джерелаOlsen, Mark Jon. "High throughput directed enzyme evolution using fluorescence activated cell sorting." 2003. http://wwwlib.umi.com/cr/utexas/fullcit?p3116133.
Повний текст джерелаLIN, GIU-XING, and 林秋杏. "Application of cascade ion exchange-affinity chromatography to separation and purification of enzymes." Thesis, 1988. http://ndltd.ncl.edu.tw/handle/94503305290031473550.
Повний текст джерелаMartinez, Rachael Elizabeth. "A novel differential extraction technique utilizing multiple enzymes: developing separation of non-sperm and sperm fractions." Thesis, 2015. https://hdl.handle.net/2144/13994.
Повний текст джерела2017-11-03T00:00:00Z
Huang, Pin-Hsiu, and 黃評脩. "Separation PL from commercial pectolytic enzymes with affinity chromatography of re-esterification CL-AIS and using this enzyme to reduce methanol in grape wine making." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/73468937052063157850.
Повний текст джерела國立屏東科技大學
食品科學系
94
Commercial pectolytic enzymes play an important role in the winemaking process. PE (pectinesterase) can catalysis the de-esterification reaction、the methyl ester group on the C6 carboxyl of D- galacturonic acid in the pectin could be hydrolyzed by PE to produce the free carboxyl group and the methanol、and the methanol is harmful to human body. Affinity chromatography re-esterification CL-AIS can separate PL from commercial pectolytic enzymes、but it was hard to separate PG (polygalacturonase) from PE. In winemaking、Black queen grape was used as the raw material in this study、and it was fermented with commercial yeast RA-17 (S. cerevisiar). Commercial pectolytic enzymes、commercial pectolytic enzymes without PL and PL were added in grape winemaking individually、Lab value、pH value、%T、titrable acidity(%)、toatal soluble solids(°Brix)、methanol and alcohol content were detected during the procedure of winemaking. The methanol content in the sample added with commercial pectolytic enzymes was 3102ppm (based on ethanol content) and the methanol content was only 563ppm as the PL enzyme was added only. The result showed that the PL (pectin lyase) enzyme used in the winemaking could reduce the methanol content and the must was clear、so、the development of PL enzyme is promising for the clarification and reducing the content of the methanol in fruit juice and wine in the future.
HOU, Wen-Chi, and 侯文琪. "I. Studies on the Linkages Between Pectin Molecules from Pea II. Studies on the Separation and Reaction Mechanisms of Pectinesterase and Pectic Acid Methylating Enzymes from Pea Plants." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/10292430806861317530.
Повний текст джерелаHou, Wen-Qi, and 侯文琪. "I. Studies on the linkages between pectin molecules from pea plants II. Studies on the separation and reaction mechanisms of pectinesterase and pectic acid methylating enzymes from pea plants." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/43764041293033439547.
Повний текст джерелаKnight, Matthew. "Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol." Thesis, 2003. https://vuir.vu.edu.au/15621/.
Повний текст джерелаLI, TAI-XING, and 李泰興. "Characteristics in enzyme separation and purification of affinity chromatography." Thesis, 1986. http://ndltd.ncl.edu.tw/handle/14976640427337227344.
Повний текст джерелаLiao, Yu-Chieh, and 廖玉潔. "Investigation of Enzyme Separation and Chromatographic Analysis of a Chemotherapeutic Drug." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/32494766912599946806.
Повний текст джерела國立成功大學
化學工程學系碩博士班
93
This thesis is divided into two parts. The first part is aimed at the investigation of immobilized metal ion affinity adsorbent for a-amylase separation and the second part is on the development and validation of analysis methods, liquid chromatography and capillary electrophoresis, for a novel chemotherapeutic enhancer, methoxyamine. b-Cyclodextrin (b-CD) and epichlorohydrin (EPI) were chosen as the matrix material and crosslinker, respectively. Thus the cross-linked b-cyclodextrin (b-CDcl) could react with iminodiacetic acid (IDA) to form b-CDcl-IDA. The immobilized metal ion adsorbent, b-CDcl-IDA-Cu2+, was prepared by chelating metal ions with the carboxylic groups from IDA. The adsorbent could adsorb 750 U/mL Bacillus licheniformis a-amylase (BLA) within 2 min. However, it took 2 hours to reach the adsorption equilibrium as the BLA activity was raised to 38,900 U/mL. A rapid and repeated BLA adsorption-desorption procedure was executed 50 times with 500 mg of adsorbent and the average recovery of 97% was achieved. In total, 93% of recovery and 46-fold concentration could thus be obtained. The presence of PEG could facilitate a-amylase adsorption from Bacillus amyloliquefaciens a-amylase (BAA) fermentation broth. 95% of adsorption could be obtained by changing the molecular weight and the amount of PEG. Adding 200 mM NaCl to the desorption agent, imidazole, the desorption of BAA raised by 35% to more than 95%. The BAA were separated from the fermentation broths by the prepared adsorbents and the BAA recovery were higher than 80%. Esterase was used to examine the specific affinity of adsorbents for a-amylase. In the mixture of a-amylase/esterase, the adsorption of a-amylase was nearly not influenced by esterase. On the contrary, the esterase adsorption was inhibited by a-amylase. In the second part, UV-Visible photometer, LC-MS and chemical reduction were used to discover the derivatization reaction of derivating agent, 4-(diethylamino)benzaldehyde (DEAB), with methoxyamine. The high performance liquid chromatography (HPLC) method quantitated the main derivate, 4-(diethylamino)benzaldehyde o-methoxyloxime (DBMO), at the wavelength of 310 nm. A linear calibration range of 0.10-10.0 mM was obtained. Another method is using pH 2.5 phosphate buffer as the separation buffer for capillary electrophoresis (CE). The relative peak area of protonated 4-(diethylamino)benzaldehyde o-methoxyloxime (DBMOH+) to the internal standard, N,N-dimethyl-p-toluidine (DMPT), was adopted to the calibration which had a linear range of 5.0-500 mM at the wavelength of 200 nm.
Huang, Shih-Hung, and 黃世宏. "Applications of iron oxide magnetic nanoparticles in enzyme immobilization and separation." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/91512743779856321288.
Повний текст джерела國立成功大學
化學工程學系碩博士班
91
This thesis concerns the applications of iron oxide magnetic nanoparticles in enzyme immobilization and separation. In the former, Lipase was immobilized on Fe3O4 magnetic nanoparticles. The preparation conditions, product properties, and the performances in the water systems were investigated. In the latter, polyacrylic acid (PAA) was covalently bound onto Fe3O4 magnetic nanoparticles to be a novel nano-adsorbent. The preparation conditions, product properties, and the application in the adsorption of Bromelain in aqueous solution were investigated. Lipase was covalently bound onto Fe3O4 magnetic nanoparticles (12.7 nm) via carbodiimide activation. The Fe3O4 magnetic nanoparticles were prepared by co-precipitating Fe2+ and Fe3+ ions in an ammonia solution and treating under hydrothermal conditions. The analyses of transmission electron microscopy (TEM) and X-ray diffraction (XRD) showed that the size and structure of magnetic nanoparticles had no significant changes after enzyme binding. Magnetic measurement revealed the resultant lipase-bound magnetic nanoparticles were superparamagnetic with a saturation magnetization of 61 emu/g (only slightly lower than that of the naked ones (64 emu/g)), a remanent magnetization of 1.0 emu/g, and a coercivity of 7.5 Oe. The analysis of Fourier transform infrared (FTIR) spectroscopy confirmed the binding of lipase onto magnetic nanoparticles. Compared to the free enzyme, the bound lipase exhibited a 1.41-fold enhanced activity, a 31-fold improved stability, and better tolerance to the variation of solution pH . The kinetic behavior of bound Lipase was also determined in aqueous solution. A novel magnetic nano-adsorbent was prepared by covalently binding polyacrylic acid (PAA) on Fe3O4 magnetic nanoparticles (13.2 nm) via carbodiimide activation. From the analyses of TEM, XRD and magnetism, the magnetic nanoparticles showed no change in size, structure and superparamagnetic characteristics after binding PAA. The analyses of FTIR, thermogravimetric analysis (TGA), differential thermal analysis (DTA) and X-ray photoelectron spectroscopy (XPS) confirmed the binding of PAA to magnetic nanoparticles and suggested the binding mechanism of PAA. The ionic exchange capacity of the resultant magnetic nano-adsorbents was estimated to be 1.64 meq/g, much higher than those of the commercial ionic exchange resins. The full recovery of bromelain was achievable within 1 min onto the nano-adsorbents at pH 3-5 and 0.1 M phosphate when the concentration of bromelain was 6 mg/ml. Moreover, the complete desorption of bromelain from the nano-adsorbents was attained within 1 minute at pH 7 when the concentration of KCl was above 0.6 M. The isothermal adsorption indicated that the adsorption behavior of bromelain followed the Langmuir adsorption isothermal and the values of the maximum amount of adsorbed bromelain (qm) and Langmuir constant (K) were 0.476 mg/mg and 58.4 ml/mg, respectively. In addition, bromelain retained 87.4% activity after adsorption/desorption.
LAI, JIE-YONG, and 賴傑勇. "Application of aqueous two-phase partition chromatography to separation and purification of enzyme." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/49547195234807262287.
Повний текст джерелаChi, Li-Lun, and 紀立倫. "Study and Fabrication on Novel Separative Structure of Enzyme-modified Field Effect Transistor." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/44373089669517176854.
Повний текст джерела中原大學
電子工程學系
87
More recently the ion sensitive field effect transistor (ISFET) has been studied extensively because of some advantage, such as small size, rapid response and more importantly, the possibility of manufacturing by MOSFET processes. However, as far as we know, ISFET is limited by the structure for biological and chemical application. Therefore, it is important to study the structure of ISFET for useful application. In this thesis, our laboratory introduced a novel separative structure of EGFET. This structure is an improvement of extended gate structure ISFET. Tin oxide thin film was prepared by the sputtering system as a pH sensitive material which has been presented by our laboratory. The first topic is the first application of separative structure of EGFET with tin oxide thin film. The results show that separative structure of EGFET with tin oxide thin film has better characteristics for pH sensor. Another major topic in this thesis emphasized on the bio-application of the novel separative structure of EnFET. In this study, we firstly immobilized the enzyme on tin oxide pH-sensitive film, and investigated the effect of acetylcholinesterase immobilized processes to detect acetylcholine. We also discussed the parameters during the measurement for pesticide detection in the further.
Barr, Love Celina Elizabeth. "Characterisation of enzymatic reactions in coacervate-based synthetic cells." 2020. https://tud.qucosa.de/id/qucosa%3A73770.
Повний текст джерела‘Bottom-up'’ Modelle synthetischer Zellen, die Schlüsselmerkmale zellbasierten Lebens imitieren, rücken immer mehr in den Fokus. Von zentraler Bedeutung ist hier die Kompartmentbildung. Sie erst ermöglicht die räumliche und zeitliche Kontrolle biochemischer Abläufe und ist daher entscheidend bei der Entwicklung synthetischer Zellen. Bisher wurden in der Mehrzahl der synthetischen Zellmodelle klassische, membrangebundene Reaktionsräume als Modellkompartimente verwendet. Jüngste Fortschritte in der Zellbiologie belegen jedoch die Bedeutung von membranlosen Kompartimenten, die durch Flüssig-Flüssig-Phasentrennung (LLPS) gebildet werden. Es wird angenommen, dass diese membranlosen Kompartimente eine zentrale Rolle bei der Regulierung der Zellchemie spielen. Jedoch ist bisher nur sehr wenig über ihren Einfluss auf enzymatische Reaktionen bekannt und experimentell belegt. Mit dem Ziel, die Bandbreite und das Verständnis synthetischer Modelle zu erweitern, wurden in dieser Arbeit neue Methoden entwickelt und dargestellt, die membranlose Kompartmentbildung benutzen. Es wurden hierfür komplexe Koazervate eingesetzt, eine spezielle Art der LLPS, welche durch die elektrostatische Anziehung von entgegengesetzt geladenen Polymeren angetrieben wird. Diese verhältnismäßig einfachen Systeme bieten eine ideale Plattform für systematische Untersuchungen des Einflusses von membranlosen Koazervatkompartimenten auf enzymatische Reaktionen. In den Kapiteln 3 und 4 konzentrierte ich mich auf die Entwicklung eines reaktionsfähigen synthetischen Modellsystems, das die Phänomene sowohl membrangebundener als auch membranfreier Kompartmentbildung vereint. Zur Steuerung der Koazervierung innerhalb von Liposomen wurde ein pH-reaktives System verwendet, welches sich den intrinsischen pKa von kationischen Polylysin zunutze macht. Diese synthetis- che Zelle wurde im folgenden Schritt mit dem Enzym Formiat-Dehydrogenase (FDH) funktionalisiert. Ich konnte damit zeigen, dass es die Eigenschaften von Koazervaten ermöglichen, die FDH-Reaktion bei global sehr niedrigen Enzymkonzentrationen zu aktivieren. Hierbei wirken die membranlosen Koazervate in Folge einer lokal er- höhten Enzymkonzentration als Zentren gesteigerter Reaktivität. Dies geschieht durch die lokale Konzentrationserhöhung in Koazervaten, was bei LLPS auch durch den Verteilungskoeffizient beschrieben wird. Mit anderen Worten agieren diese membran- losen Kompartimente durch Sequestrierung als Reaktionszentren. Im Kapitel 5 charakterisierte ich den Einfluss von diffusivem Molekülaustausch auf die Enzymkinetik über die Koazervat-Phasengrenze hinweg. Hierbei wurden zwei Systeme miteinander verglichen. Einerseits wurde ein synthetisches Zellmodell, beste- hend aus mikrofluidisch hergestellten Wasser-in-Öl Emulsionstropfen, die Koazervate enthalten, als Modellsystem mit diffusivem Austausch zwischen den Phasen verwendet. Andererseits wurden separate, reine Koazervatphasen und reine Überstandsphasen als Modellsysteme ohne Austausch verwendet. Ich habe die FDH-Reaktion in beiden Modellsystemen untersucht und kam zu dem Schluss, dass die Kopplung der Phasen die Reaktionsgeschwindigkeiten im Vergleich zu den ungekoppelten Systemen erhöht. Bei der Kopplung wirkt die Überstandsphase als Senke, die das Produkt NADH aus den Koazervaten aufnimmt. Dies erhöht die scheinbare Reaktionsgeschwindigkeit im Überstand, während die Verringerung der NADH-Konzentration im Koazervat die Produkthemmung verringert. Dies zeigt, dass die offene Phasengrenze membranloser Kompartimente eng mit ihrer Umgebung gekoppelt ist, was als erhöhte Reaktionsraten sowohl innerhalb als auch außerhalb der Kompartimente gemessen werden kann. Schließlich untersuchte ich in Kapitel 6 die Enzymkinetik der Enzyme FDH und β- Galaktosidase in der physikalisch-chemischen Umgebung des Koazervats. Mit Hilfe von Michaelis-Menten-Experimenten in der CM-Dextran/PDDA-Bulkphase konnte gezeigt werden, dass KM und Vmax von FDH im Vergleich zum Überstand signifikant erhöht sind, wohingegen jene von β-Galaktosidase ein solches Verhalten nicht zeigen. Das führte mich zu der Hypothese, dass das negativ geladene Formiatsubstrat der FDH- Reaktion stark mit dem positiv geladenen PDDA interagiert, wodurch seine Affinität für das Enzym abnimmt. Darüber hinaus wird der ratenbegrenzende Hydridtransfer in der Umgebung des Koazervats erleichtert und es kann eine Erhöhung der Reaktionsrate beobachtet werden. Die Daten zeigen, dass abhängig vom Koazervat-Milieu die Enzymdynamik in verschiedene Richtungen gesteuert werden kann. Zusammenfassend lässt sich sagen, dass meine Arbeit reaktionsfähige, steuerbare und enzymatisch aktive synthetische Zellsysteme mit Eigenschaften membranloser Kompartmentbildung etabliert. Meine Ergebnisse deuten darauf hin, dass membranlose Kompartimente einen signifikanten Einfluss auf die Dynamik enzymatischer Reaktio- nen haben. Meine Untersuchungen eröffnen damit neuartige Wege zur Kontrolle der Reaktionsgeschwindigkeit in synthetischen Systemen und erweitern das Verständnis möglicher Funktionen membranloser Organellen in vivo. Insgesamt zeige ich, dass über- legt entworfene synthetische Zellen eine hervorragende biomimetische Plattform bieten, um Einblicke in den Einfluss von membranloser Kompartimentierung auf enzymatische Reaktionen zu gewinnen. Teile der vorgestellten Arbeit wurden als wissenschaftliche Beiträge in zwei begutachteten Journalen als Erstautor veröffentlicht.