Дисертації з теми "Enterococcus faecalis; biofilm; pH"
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Tse, Chee-choong Micheal, and 謝志聰. "Effect of ultrasonic agitation on enterococcus faecalis biofilm." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45165993.
Повний текст джерелаChittezham, Thomas Vinai. "The molecular control and biological implications of autolysis in enterococcus faecalis biofilm development." Diss., Manhattan, Kan. : Kansas State University, 2009. http://hdl.handle.net/2097/1519.
Повний текст джерелаSchurig, Tilman David [Verfasser], and Karl-Thomas [Akademischer Betreuer] Wrbas. "Der antibakterielle Effekt verschiedener Wurzelkanalfülltechniken auf Enterococcus faecalis – Biofilm in humanen Wurzelkanälen in vitro." Freiburg : Universität, 2016. http://d-nb.info/112274336X/34.
Повний текст джерелаYang, Nan. "Role of Escherichia coli curli in relation with intestinal components - mucin, Klebsiella pneumoniae and Enterococcus faecalis." Phd thesis, INSA de Lyon, 2011. http://tel.archives-ouvertes.fr/tel-00657247.
Повний текст джерелаCastro, Pedro Coimbra de Almeida Osório de. "Biofilmes em endodontia." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4386.
Повний текст джерелаIntrodução: Um biofilme é uma comunidade estruturada de células bacterianas envolvidas numa matriz constituída por substâncias poliméricas e aderido a uma superfície sólida. Esta comunidade permite um modo protegido de crescimento que permite a sobrevivência dos constituintes celulares em ambientes hostis e fornece uma tolerância aumentada a agentes antimicrobianos. Objectivo: Tentar compreender a forma como os constituintes celulares se organizam e ter um melhor conhecimento das formas de resistência antimicrobiana características da organização em biofilmes, como também de rever os métodos actualmente usados para a desinfecção no tratamento endodôntico e abordando métodos alternativos ainda em estudo. Materiais e métodos: Para tal realizou-se uma pesquisa bibliográfica nos principais motores de busca: Pubmed, B-On, SciELO, Science Direct, como também no repositório da Universidade Fernando Pessoa e da Faculdade de Medicina Dentária do Porto utilizando as palavras-chave “Biofilms”, “Apical Periodontitis”, “Enterococcus faecalis” e “Biofilm Treatment “ que foram associadas de várias formas. Desta pesquisa efectuada, entre Junho de 2014 e Julho de 2014, foram escolhidos 117 artigos em Português e Inglês dos quais foram usados 89. Resultados: A forma como actualmente procedemos à desinfecção do sistema de canais radiculares, passando pela instrumentação mecânica e irrigação química não é totalmente satisfatória no que toca a uma total erradicação dos microorganismos devido a várias limitações como a complexidade anatómica dos canais e a ecologia presente no interior dos mesmos. Conclusões: De futuro, terão que ser desenvolvidas outras estratégias antimicrobianas para suplementar as existentes. Embora estas pareçam promissoras in vitro elas carecem de estudos in vivo, os quais serão necessários no futuro para ultrapassar as várias limitações presentes no sistema de canais radiculares. Introduction: A biofilm is a structured bacterial cell community enveloped in a matrix composed of polymeric substances and attached to a solid surface. This community allows for a protected way of growth that permits the survival of the cellular components in hostile environments and provides a higher tolerance to antimicrobial agents. Objective: Trying to understand the way cellular components are organized and have a better knowledge of the antimicrobial resistances that are characteristic of the way biofilms are structured, as well as to review the currently used methods for disinfection in an endodontic treatment and to address alternative methods still in study. Materials and Methods: To this end a bibliographic research was performed on the main search engines: Pubmed, B-On, SciELO, Science Direct, and also on Universidade Fernando Pessoa and Faculdade de Medicina Dentária do Porto’s repository using “Biofilms”, “Apical Periodontitis”, “Enterococcus faecalis” and “Biofilm Treatment“ as key-words that were associated in many forms. From this research performed between June 2014 and July 2014, were selected 117 articles in Portuguese and English and from those, 89 were used. Results: The way that we currently proceed regarding the disinfection of the root canal system using mechanical instrumentation and chemical irrigation is not fully satisfactory, when it comes to the total eradication of the microorganisms present due to several limitations like the complexity of the root canal anatomy and the ecology present inside the root canal. Conclusions: In the future, other antimicrobial strategies will have to be developed to supplement the currently used ones. Although these look promising in vitro they lack in vivo studies, that will be necessary in the future to overcome the several limitations present in the root canal system.
Iyer, Vijayalakshmi Subramanian. "Role of the transcription regulator RpoN (sigma 54) in Enterococcus faecalis biofilm development, metabolism and virulence." Diss., Kansas State University, 2012. http://hdl.handle.net/2097/17150.
Повний текст джерелаDepartment of Biology
Lynn Hancock
Enterococci are the third leading cause of nosocomial infections including urinary tract infections (UTI), surgical site infections (SSI) and blood stream infections. Enterococci are also found in the gastrointestinal tracts of humans, and other mammals. We elucidated the influence of the transcriptional regulator RpoN on enterococcal biofilm formation, virulence potential and cell wall architecture and proposed a potential involvement for carbohydrate metabolism in these processes. Biofilms are held together by matrix (BM) components such as extracellular DNA (eDNA) released by cell death from a sub-population of cells. The rpoN mutant (ΔrpoN) was resistant to autolysis as well as fratricide-mediated cell death and eDNA was not detected in planktonic as well as biofilm cultures. Unlike the parental strain V583, the ΔrpoN mutant formed proteinase K sensitive biofilms, suggesting that protein as well as eDNA serves as an important matrix component. The rabbit model of endocarditis was used to assess the effect of rpoN deletion on enterococcal virulence. Rabbits infected with ΔrpoN had reduced bacterial burden in heart, blood, liver, kidney and vegetation in comparison to the parental strain. The growth defect of ΔrpoN in physiologically relevant glucose levels (5 mM) partially explains the reduced bacterial burdens observed in the virulence study. Microarray analysis of ΔrpoN showed that 10% of the genome is differentially regulated by RpoN. Deletion of rpoN also protects Enterococcus faecalis from lysis in the absence of known modulators of cellular lytic events such as O-acetylation and D-alanylation. Of the four identified enhancer binding proteins in E. faecalis, MptR regulates the RpoN-dependent mannose/glucose uptake system (MptABCD) and the ΔmptR mutant phenocopied the ΔrpoN mutant in the eDNA release and growth assays. Because MptC and MptD have been shown to be the cellular receptors for class IIa and IIc bacteriocins, we are presently testing the hypothesis that these receptors may serve as a global receptor for bacteriocins. In conclusion, our data demonstrates that alterations in the metabolic state of the bacterium, as observed in the ΔrpoN mutant could be responsible for the switch in biofilm matrix composition, and this switch in turn likely influences the virulence potential of the bacterium.
Alves, Denise Ramos Silveira. "Efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis e estrutura dentinária." Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/6229.
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Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
Objective: Evaluate the effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in infected root canals and on dentin structure. Methods: Twenty-one root canals of a sample of 24 extracted single-rooted human teeth were infected by E. faecalis for 60 days to form biofilm. The antimicrobial strategies tested were (n=3 in each group): root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group I) or 0.01% malachite green (group II); root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group III); PDT with 0.01% methylene blue without root canal preparation (group IV); root canal preparation using NiTi rotary instruments, 22.5% NaOCl and 17% EDTA irrigation, and no PDT (group V); 2.5% NaOCl irrigation with no root canal preparation, and 17% EDTA irrigation (group VI); positive control (group VII). Three roots were not infected and were used as negative controls (group VIII). Samples for microbiological tests were collected using three sterile paper points, later stored in BHI and incubated at 37o C for 48 hours at three time points: before (S1) and after (S2) root canal preparation, and after PDT application (S3). Bacterial growth was analyzed according to turbidity of culture medium, presence of bacteria, and spectrophotometric optical density (nm). Specimens were sectioned and prepared for SEM analysis of dentin structure. Results: Bacteria were found at S1, S2 and S3 in all experimental groups. Optical density of culture media at S2 and S3 in groups I, II and III were lower than at S1, but not statistically different. Optical density of culture media at S2 was 28.70% and 24.67% lower than at S1 in groups I and II; after PDT, optical density was 90.00% (group I) and 37.30% (group II) lower. In group III, it was 97.70% lower at S2 and an additional 92.00% lower after PDT. In group IV, optical density increase 3.2%. Dentin analysis after PDT revealed areas of melting and recrystallization, peritubular dentin projections, intertubular dentin erosion and fusion of dentinal tubule openings, which made dentin surface irregular. Some dentinal tubules were obliterated, and there were changes in the shape of their openings. Conclusion: PDT applied after root canal preparation using manual or rotary files was not effective in eliminating E. faecalis completely. PDT changed dentin structure and resulted in dentin melting and recrystallization, as well as in dentinal tubule erosion and obliteration.
Objetivo: Avaliar o efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis em canais radiculares infectados e sobre a estrutura dentinária. Metodologia: O estudo foi desenvolvido em vinte e quatro dentes humanos unirradiculares extraídos, dos quais vinte e um canais radiculares foram infectados com E. faecalis por 60 dias para formação de biofilme. As estratégias antimicrobianas testadas foram (n=3): preparo do canal radicular com instrumentos rotatórios de NiTi/ NaOCl 2,5%/ irrigação final EDTA 17%, e TFD com azul de metileno 0,01% (Grupo I) ou verde malaquita 0,01% (Grupo II); preparo do canal radicular com instrumentos manuais de aço inox/ NaOCl 2,5%/ irrigação final EDTA 17% e TFD com azul de metileno 0,01% (Grupo III); TFD com azul de metileno 0,01% sem preparo prévio do canal radicular (Grupo IV); preparo do canal radicular com instrumentos rotatórios de NiTi/ NaOCl 2,5%/ irrigação final EDTA 17% sem emprego da TFD (Grupo V); irrigação com hipoclorito de sódio 2,5% sem preparo do canal radicular/ irrigação final EDTA 17% (Grupo VI); controle positivo (Grupo VII). Três espécimes não foram contaminados, sendo utilizados como controle negativo (Grupo VIII). Coletas microbiológicas foram realizadas, antes (CM1) e após (CM2) o preparo do canal radicular, e depois da aplicação da TFD (CM3), utilizando três pontas de papel absorventes esterilizadas, posteriormente armazenadas em BHI e a seguir , incubadas a 37o C por 48 horas. O crescimento bacteriano foi analisado pela turbidez do meio de cultura, sendo determinada a presença ou ausência de bactérias, e pela densidade óptica do meio de cultura, interpretada por espectrofotometria (nm). A seguir, os espécimes foram seccionados e preparados para análise da estrutura dentinária por meio de imagens de MEV. Resultados: A presença de bactérias foi verificada na CM1 , CM2 e CM3 de todos os grupos experimentais. As medidas da densidade óptica dos meios de cultura das CM2 e CM3 nos grupos experimentais I, II e III apresentaram redução quando comparada a CM1, porém não significativa estatisticamente. Nos Grupos I e II a densidade óptica do meio de cultura foi reduzida em 28.70% e 24,67% em CM2, respectivamente. Após a TFD, a redução da densidade óptica foi 90,00% (Grupo I) e 37,70% (Grupo II). No Grupo III, a redução da densidade óptica do meio de cultura foi de 97,70% na CM2, com redução adicional de 92,00% após TFD. No Grupo IV foi verificado aumento da densidade óptica do meio de cultura em 3,2%. A análise da dentina evidenciou, nos grupos submetidos à TFD, áreas de derretimento e recristalização, projeção da dentina peritubular, e regiões com erosão da dentina intertubular e união das entradas dos túbulos dentinários, tornando a superfície dentinária irregular. Obliteração de túbulos dentinários com alteração do contorno de suas entradas também foi verificada. Conclusão: A TFD, após preparo do canal radicular com sistema rotatório ou manual, não foi efetiva na eliminação completa de E. faecalis, e alterou a estrutura dentinária, determinando derretimento e recristalização de dentina, erosão e obliteração de túbulos dentinários.
Rosa, Ricardo Abreu da. "Efetividade da terapia fotodinâmica associada à soluções irrigadoras frente a dois modelos de biofilme." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/144218.
Повний текст джерелаThe aim of this study was to evaluate the antibacterial effect and the biofilm dissolution promoted by photodynamic therapy (PDT) associated with 2.5% NaOCl and 2% CHX over monospecies and multispecies biofilms. In monospecies biofilm model, forty-six mandibular premolars were contaminated with Enterococcus faecalis strains (ATCC 29212) broth culture for 21 days. Specimens were divided into three groups according to the irrigant used: saline, 2% of CHX and 2.5% of NaOCl. After irrigation with 5 mL of each irrigant, PDT was performed. Samples were collected at baseline (S1), after irrigation (S2) and after PDT (S3). In multispecies biofilm model, sixty bovine dentin blocs were infected intraorally, and divided into six groups: saline, saline/PDT, CHX, CHX/PDT, NaOCl and NaOCl/PDT. Confocal laser scanning microscopy was used to assess the percentage and the biovolume of live cells and the total biovolume. All groups reduced UFC’ counts after irrigation procedures (S1-S2); however CHX and NaOCl promoted the lowest UFCs counts (P < 0.05). PDT significantly reduced the bacterial counts in saline group (S2-S3; P < 0.05). In multispecies biofilm model, the lowest amount of live cells was observed in CHX, CHX/PDT, NaOCl and NaOCl/PDT groups, with no differences among them (P > 0.05). PDT did not reduce the total biovolume (P > 0.05); however it appears to decrease the biovolume and the amount of live cells after irrigation with 2% CHX and 2.5% NaOCl. PDT associated with saline reduced the bacterial load in canal infected with E. faecalis. PDT seems to reduce the amount and the biovolume of live cells, but did not reduce the total biovolume of cells in multispecies biofilm induced in situ. Finally, the irrigant was decisive to dissolve multispecies biofilm.
Chettaoui, Rayane. "Activité anti-biofilm du cranberry et de l’un de ses métabolites envers Enterococcus faecalis dans un contexte d’infection urinaire." Thesis, Cergy-Pontoise, 2017. http://www.theses.fr/2017CERG0909.
Повний текст джерелаEscherichia coli and Enterococcus faecalis are two main pathogens involved in urinary tract infections (ITU) in town medicine and in the hospital. These bacterial species are responsible for acute UTIs with recurrence phenomena and in chronic ITUs. The consumption of antibiotics is directly correlated with the resistance of uropathogenic bacteria, which shows the importance of controlling the use of antibiotics and of developing alternative preventive and curative treatments for urinary infections.Cranberry consumption of their extracts is traditionally associated with the maintenance of healthy urinary tract. In addition, some clinical studies seem to show a preventive effect of ITUs associated with cranberry consumption. In vitro and ex vivo, the consumption of these extracts by humans reduces the adhesion of certain E. coli strains to urinary epithelial cells and biofilm formation of different species. The working hypothesis is that the consumption of cranberry extracts leads to the formation of urinary metabolites that decrease the adhesion of uropathogenic bacteria to the urinary epithelium. This mechanism would be the basis for the prevention of ITU by consumption of cranberry extracts. However, bioactive metabolites remain largely unknown
Ardalan, Cyrous. "A Comparative Study of Intraradicular Enterococcus Faecalis Biofilm Removal with Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4741.
Повний текст джерелаHüfner, Alexander [Verfasser]. "Auswirkungen von kaltem Atmosphärendruckplasma und Natriumhypochlorit auf einen Enterococcus faecalis Biofilm: Eine in vitro Studie in humanen Zähnen / Alexander Hüfner." Greifswald : Universitätsbibliothek Greifswald, 2018. http://d-nb.info/1149857579/34.
Повний текст джерелаZimmermann, Felix [Verfasser], Tina [Akademischer Betreuer] Rödig, Sabine [Gutachter] Sennhenn-kirchner, and Martin [Gutachter] Oppermann. "Antibakterielle Wirksamkeit schall- und ultraschallaktivierter Wurzelkanalspülungen auf einen Enterococcus faecalis-Biofilm / Felix Zimmermann ; Gutachter: Sabine Sennhenn-kirchner, Martin Oppermann ; Betreuer: Tina Rödig." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/116814843X/34.
Повний текст джерелаDomingues, Nádia [UNESP]. "Avaliação de extratos de plantas medicinais em biofilmes multiespécie de Candida albicans com Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis e Pseudomonas aeruginosa." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/153356.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Os micro-organismos estão cada vez mais resistentes aos medicamentos disponíveis tanto na medicina quanto na odontologia, e esta resistência é ainda maior quando estão organizados em biofilmes mono ou multiespécies, de modo que o estudo de antimicrobianos alternativos, como fitoterápicos, estão em crescente ascensão. A interação entre leveduras e bactérias está intimamente presente na cavidade bucal, em que nichos como dentes, língua, mucosa e bolsa periodontal, nutrientes e temperatura adequados promovem condições favoráveis para formação do biofilme. Com isso, o objetivo deste trabalho foi avaliar a atividade antimicrobiana dos extratos de Pfaffia paniculata K (pfaffia), Hamamelis virginiana L. (hamamelis), Stryphnodendron barbatiman (barbatimão) e Gymnema sylvestre (gimena) em biofilmes heterotípicos de Candida albicans (ATCC 18804) com Streptococcus mutans (ATCC 35688), Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 4083) e Pseudomonas aeruginosa (ATCC 15442). Para isso, suspensões padronizadas em 107 cels/mL dos micro-organismos testes, foram distribuídos em placas de microtitulação de 96 poços, juntamente 100 µL de caldo BHI. As placas foram incubadas em estufa bacteriológica a 37ºC/48h (5% de CO2 para S. mutans) e, após, os biofilmes foram submetidos ao tratamento com os extratos por 5 min e 24 h, nas respectivas concentrações de 100 mg/mL, 50 mg/mL e 25 mg/mL. Foi utilizado solução salina 0,9% (5 min) ou caldo BHI (24 h) nos grupos controles. Após, os biofilmes foram lavados e desagregados do fundo da placa e diluições seriadas foram semeadas em ágar seletivo para cada micro-organismo. Foram realizadas contagens de UFC/mL (log10) após 24 h de incubação e analisadas estatisticamente pelo método Kruskal-Wallis suplementado pelo teste de Dun’s (p<0.05). Os resultados obtidos indicaram reduções significativas promovidas pelos extratos nos dois tempos de tratamento analisados. Foi observado que o extrato de H. virginiana apresentou redução de todos os grupos analisados no tempo de tratamento de 24 h. Conclui-se que os extratos de P. paniculata, H. virginiana, S. barbatiman e G. sylvestre apresentaram ação antimicrobiana sobre biofilmes muliespécie de C. albicans com as bactérias de interesse médico-odontológico, nos tempos de tratamento de 5 min e 24 h.
Microorganisms are increasingly resistant to drugs available in both medicine and dentistry, and this resistance is even greater when they are organized into mono or multispecies biofilms, so that the study of alternative antimicrobials, such as herbal medicines, are on the rise. The interaction between yeasts and bacteria is intimately present in the oral cavity, in which niches such as teeth, tongue, mucosa and periodontal pocket, food and adequate temperature promote adequate conditions for biofilm formation. The aim of this study was to evaluate the antimicrobial activity of extracts of Pfaffia paniculata K., Hamamelis virginiana L., Gymnema sylvestre and Stryphnodendron barbatiman M. in heterotopic biofilms of Candida albicans (ATCC 18804) with Streptococcus mutans (ATCC 35688). Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 4083), and Pseudomonas aeruginosa (ATCC 15442). Standardized suspensions at 107 cells/mL of the test microorganisms were distributed in 96 well microtiter plates together with 100 μL of BHI broth, the plates were incubated in a bacteriological oven at 37°C/48h (5% CO2 for S. mutans). After the incubation time, treatments were performed at 5 min and 24 h times, applying the respective extracts at the concentrations of 100 mg, 50 mg and 25 mg/mL and applying 0.9% saline solution or BHI broth in the control groups. After the biofilms were washed and disaggregated from the bottom of the plate, performing serial dilutions for later seeding in selective agar. UFC/ml (log10) counts were performed after 24 h of incubation and statistically analyzed Kruskal-Wallis method supplemented by the Dun's test (p<0.05%). The obtained results indicated significant reductions promoted by extracts in the two treatment times analyzed. It was observed that the extract of H. virginiana showed reduction of all the analyzed groups without treatment time of 24 h. It is concluded that the extracts of P. paniculata, H. virginiana, S. barbatiman and G. sylvestre presented antimicrobial action after analysis of the heterotypic biofilms of C. albicans with the bacteria of medical and dental interest, in the treatment times of 5 min and 24 h.
Thieme, Lara [Verfasser], Mathias [Gutachter] Pletz, Michael [Gutachter] Bauer, and Annette [Gutachter] Moter. "Evaluation of treatment options for Enterococcus faecalis endocarditis by assessing synergistic and anti-biofilm effects / Lara Thieme ; Gutachter: Mathias Pletz, Michael Bauer, Annette Moter." Jena : Friedrich-Schiller-Universität Jena, 2021. http://d-nb.info/1238142214/34.
Повний текст джерелаSenechal, Annie. "Initial stages of adhesion of enterococcus faecalis responsible for biofilm formation on medical devices : quantification of bacterial adhesion and measurement of cell-surface interaction forces." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80142.
Повний текст джерелаThe results showed significant differences in E. faecalis adhesion between the studied polymers suggesting that precautions should be made for the choice of polymers in the design of medical applications in order to prevent subsequent biofilm formation.
Cambronel, Melyssa. "Analyse de la réponse physiologique et génétique de Pseudomonas aeruginosa et Enterococcus faecalis envers l'épinéphine et recherche de senseurs putatifs. Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103 Influence of catecholamines (epinephrine/norepinephrine) on biofilm formation and adhesion in pathogenic and probiotic strains of Enterococcus faecalis Evaluation of probiotic properties and safety of Enterococcus faecium isolated from artisanal Tunisian meat "dried ossban" Probiotic potential and safety evaluation of Enterococcus faecalis OB14 and OB15, isolated from traditional Tunisian Testouri cheese and Rigouta, using physiological and genomic analysis Draft genome sequence of Enterococcus faecalis strain OB15, a probiotic strain recently isolated from "Tunisian Rigouta Cheese"." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR106.
Повний текст джерелаMicrobial endocrinology studies the interactions that can occur between bacteria and molecules secreted by the host. Pseudomonas aeruginosa and Enterococcus faecalis are two opportunistic pathogens involved in nosocomial infections. Their presence in the human body puts them in contact with eukaryotic molecules, including catecholamines (epinephrine andnorepinephrine) which are produced in the event of a "Fight of Flight" response but also during trauma or surgical procedures. These substances are known to be able to modulate bacterial physiology, including virulence and biofilm formation. The present study has shown that epinephrine could modulate the physiology of P. aeruginosa and E. faecalis probiotic and pathogenic strains. Epinephrine stimulates, among other things, biofilm formation andadherence, and could play, in P. aeruginosa, the role of xenosiderophore, thus promoting the development of infection by iron intake. We also hypothesized the presence of putative adrenergic sensors in P. aeruginosa and E. faecalis, PmrB and VicK, respectively. This work has increased our knowledge on host/pathogen communication and the effect of epinephrine on bacteria within the human microbiota. This may help in the future to discover novel therapeutic targets
Vasconcelos, Rodrigo Arruda. "Estudo do pH e atividade antimicrobiana sobre Enterococcus faecalis de medicação intracanal à base de hidróxido de cálcio associada ao óleo de melaleuca, clorexidina ou farnesol /." Araraquara, 2016. http://hdl.handle.net/11449/138887.
Повний текст джерелаBanca: Denise M Spolidorio
Banca: Flaviana Bombarda de Andrade
Resumo: Medicações intracanal (MIC) são utilizadas para complementar a desinfecção do sistema de canais radiculares. A associação de antimicrobianos ao hidróxido de cálcio visa aumentar sua ação. As MIC (Capítulo 1) hidróxido de cálcio (HC)/óleo de melaleuca (OM) 4,5%/polietilenoglicol 400 (PL), HC/OM 9%/PL, HC/CLX 0,4%/PL e HC/PL (Capítulo 2) HC/farnesol (FAR) 4,5%/PL, HC/FAR 9%/PL e HC/PL foram analisadas. O teste de contato direto sobre células planctônicas (Capítulos 1 e 2) foi realizado utilizando suspensões das MIC. O teste de contato direto das MIC sobre briofilme (Capítulo 2) foi realizado em blocos de dentina bovina (5mm x 5mm x 0,7mm) com biofilme de Enterococcus faecalis induzidos por 15 dias e contato com as suspensões das MIC por 6 horas. A efetividade antibacteriana (Capítulos 1 e 2) foi avaliada em canais radiculares de dentes de humanos extraídos. Canais radiculares de dentes unirradiculares padronizados em 15mm foram preparados até MTwo #40/.04 e contaminados com E. faecalis por 21 dias. As MIC foram mantidas no canal durante 7 dias. A primeira coleta (C1) foi realizada após período de contaminação, a segunda coleta (C2), imediatamente após a remoção das MIC e terceira coleta (C3), realizada 7 dias após a remoção das MIC. Foi realizada contagem de UFCmL-1. O pH foi avaliado após preenchimento de tubos de polietileno (Capítulos 1 e 2) com as pastas por 12 horas, 1, 3, 7, 14, 21 e 28 dias. A difusão de íons hidroxila por meio de dentina bovina (Capítulos 1 e 2) foi ava... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Intracanal medications (ICM) are used to complement desinfection of the root canal system. The association of antimicrobials to calcium hydroxide aims to improve its action. The ICM (Chapter 1) calcium hydroxide (CH)/tea tree oil (TTO) 4.5%/polyethylene glycol 400 (PL), CH/TTO 9%/PL, CH/chlorhexidine (CHX) 0.4%/PL e CH/PL (Chapter 2) CH/farnesol (FAR) 4.5%/PL, CH/FAR 9%/PL and HC/PL were analysed. Direct contact test against E. faecalis planktonic cells (Chapters 1 and 2) was performed by using suspension of ICM. Direct contact test on biofilm (Chapter 2) was performed on bovine dentin blocks (5mm x 5mm x 0.7mm) with E. faecalis biofilms induced by 15 days and contact with the suspensions of ICM for 6 hours. Antibacterial effectiveness was evaluated in root canals of extracted human teeth (Chapters 1 e 2). Standardized single-rooted teeth with 15mm were prepared up to #40/.04 MTwo and contaminated with E. faecalis for 21 days. As ICM were kept within the root canals for 7 days. The first sample (S1) was performed after contamination, S2 immediately after the ICM removal and S3 7 days after the ICM removal.It was performed UFCmL-1 count. pH was evaluated after filling the polyethylene tubes (Chapter 1 and 2) with the ICM for 12 hours, 1, 3, 7, 14, 21 and 28 days. The diffusion of hydroxyl ions through the bovine dentin (Chapters 1 and 2) was evaluated after filling root canals of bovine teeth after 1, 3, 7, 14, 21, 28 and 60 days. Data was statistically analyzed using ANOVA an... (Complete abstract click electronic access below)
Mestre
Endres, Sarah [Verfasser], Tina [Akademischer Betreuer] [Gutachter] Rödig, and Ralf [Gutachter] Bürgers. "Antibakterielle Wirksamkeit der photodynamischen Therapie bei verschiedenen Insertionstiefen einer LED-Lichtquelle anhand eines Enterococcus faecalis-Biofilm-Modells / Sarah Endres ; Gutachter: Tina Rödig, Ralf Bürgers ; Betreuer: Tina Rödig." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1139170600/34.
Повний текст джерелаGómez, Loayza Carmen Rocio. "Evaluación in vitro de la eficacia antimicrobiana del Hipoclorito de Calcio al 2,5% y el Hipoclorito de Sodio al 2,5% sobre un Biofilm de Enterococcus faecalis y Candida albicans." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2017. https://hdl.handle.net/20.500.12672/6313.
Повний текст джерелаTesis
Gómez, Loayza Carmen Rocio, and Loayza Carmen Rocio Gómez. "Evaluación in vitro de la eficacia antimicrobiana del Hipoclorito de Calcio al 2,5% y el Hipoclorito de Sodio al 2,5% sobre un Biofilm de Enterococcus faecalis y Candida albicans." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2017. http://cybertesis.unmsm.edu.pe/handle/cybertesis/6313.
Повний текст джерелаTesis
Hoffmann, Carolin Yvonne [Verfasser], Tina [Akademischer Betreuer] Rödig, Ralf [Akademischer Betreuer] Bürgers, and Rainer [Akademischer Betreuer] Mausberg. "In-vitro-Analyse der antimikrobiellen Effektivität von Octenidol, Natriumhypochlorit und Chlorhexidin gegen Enterococcus faecalis anhand eines intrakanalären Biofilm-Modells / Carolin Yvonne Hoffmann. Betreuer: Tina Rödig. Gutachter: Tina Rödig ; Ralf Bürgers ; Rainer Mausberg." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://d-nb.info/1085592375/34.
Повний текст джерелаEberl, Monika Diana [Verfasser], Tina [Akademischer Betreuer] Rödig, Tina [Gutachter] Rödig, and Sabine [Gutachter] Sennhenn-Kirchner. "Vergleich der antibakteriellen Effektivität vier unterschiedlicher Techniken zur Aktivierung der Wurzelkanalspülung (Hand, Ultraschall, RinsEndo®, EndoVac®) auf Enterococcus faecalis anhand eines Wurzelkanal-Biofilm-Modells / Monika Diana Eberl ; Gutachter: Tina Rödig, Sabine Sennhenn-Kirchner ; Betreuer: Tina Rödig." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1153606968/34.
Повний текст джерелаStenhouse, Mark. "Investigation of the effect of rapid and slow external pH increases on Enterococcus faecalis biofilm grown on dentine." Thesis, 2011. http://hdl.handle.net/2440/71967.
Повний текст джерелаThesis (D.Clin.Dent.) -- University of Adelaide, School of Dentistry, 2011
Chang, Shyang-Yuan, and 張祥源. "Biofilm formation enhances Enterococcus faecalis survived after treatment with irrigants." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/58159284051027849678.
Повний текст джерела中國醫藥大學
牙醫學系碩士班
101
Enterococcus faecalisis the most important pathogen leading to persistent and secondary infections of root canal-treated teeth. The formation of biofilm protects E. faecalis from the eradication by intracanal medicaments. The aim of this study was to investigate the role of biofilm in E. faecalis isolated from patients with root canal retreatment in impeding irrigants–chlorhexidine (CHX) and sodium hypochlorite (NaOCl). A total of 13 E. faecalis were isolated from patients with failed root canals retreatment and were enrolled in this study. Microbiological investigation of available E. faecalis isolates was performed by bacterial biofilm assay and morphological observation. Our results showed that the activities of biofilm formation by the E. faecalis isolated from root canals were highly diverse. E. faecalis with higher biofilm activity exhibited more resistant to irrigants than lower biofilm formation strain. Confocal laser scanning microscopy (CLSM) observation showed that bacterial biofilms were intact of higher biofilm E. faecalis upon treated with CHX. These results demonstrated that E. faecalis harbors biofilm formation activity that encapsulated bacteria in extracellular polysaccharide, which may lead to enhanced bacteria resistant to irrigant eradicate, subsequently induced persistent infection in canal roots.
Plutzer, Barbara. "Comparative efficacy of endodontic medicaments against Enterococcus faecalis biofilms." 2009. http://hdl.handle.net/2440/56427.
Повний текст джерелаThesis (D.Clin.Dent.) -- University of Adelaide, School of Dentistry, 2009
Kwan, Daryl A. "Reduction of enterococcus faecalis biofilm by blue light and sodium hypochlorite." Thesis, 2017. https://doi.org/10.7912/C2465N.
Повний текст джерелаIntroduction: Microbial biofilms have been shown to be a cause of persistent endodontic infections. It is more resistant than planktonic bacteria to host immune defenses and antimicrobials. Studies indicate that photodynamic light therapy (PDT), which involves using light at specific wavelengths, has a potent antibacterial effect on bacterial biofilm. PDT is an antimicrobial strategy that involves the use of a nontoxic photosensitizer (PS) along with a light source. The excited PS reacts with molecular oxygen to produce highly reactive oxygen species, which induce injury or death to microorganisms. PSs have a high degree of selectivity for inhibiting microorganisms without negatively affecting host mammalian cells. PDT has been suggested as an adjuvant to conventional endodontic treatment. Studies at IUSD have shown that blue light at 380 nm to 440 nm has the ability to inactivate Streptococcus mutans biofilm without any exogenous PS. Objective: The objective of this study was to determine the effectiveness of blue light at 380 nm to 440 nm to reduce adherence of Enterococcus faecalis biofilm after NaOCl irrigation at various concentrations. Materials and Methods: E. faecalis biofilm was established for 72 hours in 96- well flat-bottom microtiter plates using Tryptic Soy Broth supplemented with 1.0-percent sucrose (TSBS). Biofilm was irradiated with blue light for 5 minutes before exposure to various concentrations of NaOCl for 30 seconds. A crystal violet biofilm assay was used to determine relative density of the biofilm. Data were analyzed with two-way ANOVA and Sidak-adjusted multiple comparisons using a 5.0-percent significance level. Null Hypothesis: Blue light and NaOCl will not have an effect against E. faecalis biofilm adherence. Results: Overall, there was a significant effect (p < 0.05) for NaOCl and a significant effect for blue light. The effects of the combination of NaOCl and blue light were also significant. Conclusion: We reject the null hypothesis and accept the alternative hypothesis that blue light when used in conjunction with NaOCl will reduce adherence of E. faecalis biofilm.
Zimmermann, Felix. "Antibakterielle Wirksamkeit schall- und ultraschallaktivierter Wurzelkanalspülungen auf einen Enterococcus faecalis-Biofilm." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E4A5-F.
Повний текст джерелаKindler, Justin K. "Use of electromagnetic stimulation on an Enterococcus faecalis biofilm in root canal treated teeth in vitro." Thesis, 2019. http://hdl.handle.net/1805/19654.
Повний текст джерелаIntroduction: Nonsurgical root canal therapy procedures aim to reduce the total microbial load within an infected root canal system through chemomechanical debridement of the root canal system via instrumentation in conjunction with an antibacterial irrigating solution. The most commonly used irrigant is sodium hypochlorite, often at concentrations toxic to human cells. Electromagnetic wave irradiation is a novel method of disinfection that has been shown to be bactericidal against planktonic microorganisms in solution, but its efficacy against an established biofilm is unknown. Pilot studies have demonstrated a synergistic killing effect with sodium hypochlorite through a process termed electromagnetic stimulation (EMS). If confirmed, lower concentrations of the current gold standard of 6.0-percent sodium hypochlorite could be used to irrigate infected root canals during endodontic treatment, resulting in less toxicity to human cells. There are also regenerative implications as EMS could be used to disinfect the root canals of immature teeth using 1.5-percent sodium hypochlorite, as recommended by the American Association of Endodontists. Objectives: The purpose of this in-vitro study was to evaluate the anti-biofilm effect of EMS against an established biofilm of Enterococcus faecalis. Materials and Methods: Single rooted teeth were cut to a standardized length (12 mm) and instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with E. faecalis, which grew for two weeks to form an established biofilm. There were five treatment groups: 1) 6.0-percent sodium hypochlorite; 2) 1.5-percent sodium hypochlorite; 3) 1.5-percent sodium hypochlorite with EMS; 4) 0.9-percent saline with EMS and 5) 0.9-percent saline. Samples were collected, plated, and incubated for two days. The number of CFUs/mL was determined and converted to log10. The effect of treatment group on bacterial counts was made using Wilcoxon Rank Sums Test. One sample per group was scored and split for confocal imaging. Null Hypothesis: Teeth treated with EMS in combination with 1.5-percent sodium hypochlorite or 0.9-percent saline will not demonstrate a significant anti-biofilm effect in comparison to those treated with 6.0-percent sodium hypochlorite alone. Results: 0.9-percent saline and 0.9-percent saline with EMS were significantly higher than 6.0-percent NaOCl, 1.5-percent NaOCl, and 1.5-percent NaOCl with EMS. 0.9-percent saline was significantly higher than 0.9-percent saline with EMS. The three groups that included treatment with NaOCl were not significantly different from each other. Confocal imaging confirmed the CFU findings. Conclusion: Because there was no growth in any of the NaOCl groups, the null hypothesis cannot be rejected. However, there was an antibiofilm effect when comparing the two saline groups, demonstrating that EMS has an antibiofilm effect. Future studies should focus on determining what concentration of NaOCl is most effective in combination with EMS.
Seet, Aaron Nicholas. "An in-vitro evaluation of the effectiveness of endodontic irrigants, with and without sonic and laser activation, in the eradication of Enterococcus faecalis biofilm." Thesis, 2011. http://hdl.handle.net/2440/70448.
Повний текст джерелаThesis (D.Clin.Dent.) -- University of Adelaide, School of Dentistry, 2011
Broady, Adam B. "Effectiveness of ozonated water irrigation against an established Enterococcus faecalis biofilm in root canal treated teeth in vitro." Thesis, 2020. http://hdl.handle.net/1805/23181.
Повний текст джерелаIntroduction: One of the main objectives of endodontic therapy is to reduce microbes and remove inflamed pulpal tissue within the root canal system (RCS). This is accomplished through chemomechanical debridement of the RCS using hand and rotary instrumentation along with an antimicrobial irrigant. Today, the most commonly used irrigant is sodium hypochlorite (NaOCl), often at concentrations toxic to human cells. The use of ozone as an endodontic irrigant is a novel technique that has been proven to be antimicrobial against several microorganisms. However, independent research is lacking on ozone’s efficacy against an established endodontic biofilm. If ozone’s efficacy against biofilms is confirmed, the use of toxic and potentially dangerous sodium hypochlorite could be replaced in some clinical situations (i.e., regeneration, immature teeth, resorption) with a safer and effective alternative. Objective: The aim of the current study was to evaluate the anti-biofilm activity of different concentrations of ozonated water compared to various concentrations of NaOCl against an established endodontic biofilm of Enterococcus faecalis in root canal treated teeth in vitro. Materials and Methods: The crowns of similarly sized, maxillary anterior teeth were removed, and the roots cut to a standard length (12 mm). All root canals were instrumented to a standard size. Specimens were sterilized and then inoculated with E. faecalis, which were allowed to grow for two weeks to form an established biofilm. There were six treatment groups: 1) 6% NaOCl; 2) 1.5% NaOCl; 3) 16µg/mL ozonated water; 4) 25µg/mL ozonated water; 5) 50µg/mL ozonated water, and 6) saline. Following treatment, samples were collected, plated, and incubated for two days. The number of CFU/mL were determined, and samples visualized using confocal imaging. The effect of treatment group on bacterial counts was made using one-way ANOVA followed by pair-wise comparisons. Null Hypothesis: Endodontically treated teeth irrigated with ozonated water will not demonstrate a statistically significant decrease in the E. faecalis biofilm compared to those treated with sodium hypochlorite Results: CFUs were converted to log10 and compared using Fisher’s Exact tests or one-way ANOVA followed by pair-wise tests. In all observations utilizing NaOCl irrigation, no colonies formed following treatment. The two NaOCl groups, with 0 CFU/mL, were significantly different than the other four groups (p=0.009). Saline showed a trend towards higher CFU/mL than 50 µg/ml O3 (p=0.068). None of the other comparisons approached statistical significance (p=0.453 25 µg/ml O3, p=0.606 16 µg/ml O3, p=0.999 25 µg/ml O3 vs 50 µg/ml O3, p=0.990 16 µg/ml O3 vs 50 µg/ml O3, p=1.000 16 µg/ml O3 vs 25 µg/ml O3). Confocal imaging helped illustrate effects of irrigation and confirm CFU findings. Conclusion: The results of this study failed to reject the null hypothesis. There was a statistically significant difference in the E. faecalis biofilm remaining in the groups treated with ozonated water compared to those treated with NaOCl. However, there was a trend towards higher CFU/mL in the saline group compared to the 50µg/mL ozonated water group. According to this finding, future studies should evaluate the effects of higher concentrations of ozonated water against an established E. faecalis biofilm. In addition, other follow-up studies might include ozonated water’s effect on human cells, such as the stem cells of the apical papilla that are so critical to the success of regenerative endodontic procedures. Due to university and laboratory closures caused by the COVID-19 pandemic, this project was stopped short and an insufficient sample size did not allow for proper statistical power. Additional occasions should be run upon the university’s re-opening to allow for proper statistical power.
Endres, Sarah. "Antibakterielle Wirksamkeit der photodynamischen Therapie bei verschiedenen Insertionstiefen einer LED-Lichtquelle anhand eines Enterococcus faecalis-Biofilm-Modells." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3EC9-B.
Повний текст джерелаHaslam, Bryce S. "The antibacterial effect of a radiopaque double antibiotic paste against both an established multispecies and a single enterococcus faecalis biofilm." Thesis, 2019. http://hdl.handle.net/1805/19637.
Повний текст джерелаFor regenerative endodontic procedures (REPs) to be successful an elimination of bacteria from the root canal system must be accomplished. Many different medicaments with antibacterial properties have been used to obtain complete disinfection. Double antibiotic paste (DAP) containing a mixture of ciprofloxacin and metronidazole has been shown to be a promising intracanal medicament. The addition of a radiopaque filler such as zirconium oxide to DAP may affect the antibacterial properties of DAP as well as allow precise placement and radiographic visualization of its position in the canal system. The aim of the proposed study was to evaluate the direct antibacterial properties of zirconium oxide radiopacifier combined with DAP (RoDAP) against a multispecies biofilm from a bacterial isolate from an infected immature tooth with a necrotic pulp and a known single species biofilm. 4x4 mm radicular dentin specimens (n = 112) obtained from human extracted teeth were used prepared and sterilized prior to use. A multispecies clinical bacterial isolate from an immature tooth with a necrotic pulp and a single species Enterococcus faecalis isolate were obtained. These bacterial isolates were used to inoculate dentin slabs and grown for 3 weeks. The dentin slabs were treated for 1 week with 1.0-mg/mL and 10- mg/mL RoDAP, 1.0-mg/mL DAP, and two placebo pastes consisting of methyl cellulose (MC) and methyl cellulose combined with zirconium oxide (RoMC), respectively, as well as two no-treatment controls. Following treatment, the grown biofilm was detached and spiral plated. The plated biofilm cells were cultured for 24 hours and each group examined using a colony counter to determine bacterial numbers (CFUs/mL). Data analysis, using a 5.0-percent significance level was conducted using one-way ANOVA followed by pair-wise group comparisons. Both 1.0-mg/mL and 10 mg/mL RoDAP demonstrated significant antibacterial effects against bacterial isolates from an immature tooth with a necrotic pulp as well as an E. faecalis isolate. The precise application of RoDAP confirmed radiographically with its direct antibacterial properties may be beneficial for intracanal disinfection during REPs.
Hoffmann, Carolin Yvonne. "In-vitro-Analyse der antimikrobiellen Effektivität von Octenidol, Natriumhypochlorit und Chlorhexidin gegen Enterococcus faecalis anhand eines intrakanalären Biofilm-Modells." Doctoral thesis, 2016. http://hdl.handle.net/11858/00-1735-0000-0028-86C8-9.
Повний текст джерелаMüller, Nada [Verfasser]. "Siedeln sich Enterococcus-faecalis-Isolate von Patienten mit periradikulären Läsionen im In-vitro-Biofilm aus Speichelbakterien an? / vorgelegt von Nada Müller." 2009. http://d-nb.info/1001904923/34.
Повний текст джерелаBrothers, Kara M. "Use of Electromagnetic Stimulation in Combination with Low Concentration Sodium Hypochlorite on an In Vitro Enterococcus Faecalis Biofilm on Root Canal Treated Teeth." Thesis, 2021. http://dx.doi.org/10.7912/C2/26.
Повний текст джерелаIntroduction: A novel device developed by J. Morita can generate electromagnetic stimulation (EMS) into the root canal. Objectives: The purpose of this study was to determine the anti-biofilm effect of EMS combined with low concentrations of NaOCl against an established biofilm of Enterococcus faecalis in an in vitro human tooth model. Materials and Methods: Single rooted human teeth were standardized and an E. faecalis biofilm was established in the canal. The specimens were subject to six treatment groups: 1) 1.5% NaOCl; 2) 1.5% NaOCl and EMS; 3) 0.25% NaOCl; 4) 0.25% NaOCl and EMS; 5) saline and 6) saline and EMS. Biofilm was collected, plated, and the number of colony forming units (CFU)/mL was used to determine antibacterial activity. Results: The effect of treatment group on bacterial counts were made using one-way ANOVA followed by pair-wise comparisons. Although there was no significant difference between individual groups tested, there was statistically significant difference between the average difference between ‘treatments with EMS’ and ‘treatments without EMS.’ Conclusion: EMS can improve the antibacterial efficacy of NaOCl against an established biofilm of E. faecalis in an in vitro human tooth model
Eberl, Monika Diana. "Vergleich der antibakteriellen Effektivität vier unterschiedlicher Techniken zur Aktivierung der Wurzelkanalspülung (Hand, Ultraschall, RinsEndo®, EndoVac®) auf Enterococcus faecalis anhand eines Wurzelkanal-Biofilm-Modells." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E32F-5.
Повний текст джерелаMagalhães, Andreia Manuela Pereira. "Medicação intracanalar em endodontia: revisão de literatura." Master's thesis, 2018. http://hdl.handle.net/10284/7487.
Повний текст джерелаEndodontics is an area of Dental Medicine that allows the disinfection and consequent control of the infectious focus in the oral cavity. This treatment has as steps, instrumentation, irrigation and obturation that can be performed in a single session but its instrumentation and irrigation may not be enough, thus arising the importance of the use of intracanal medication. It is entended a literature review and collection of information about the subject in computer databases such as B-on, Pubmed, PubMed, B-On, "Google academic", Journal of Endodontics and in the books Caminhos da Polpa and Prep Manual for Undergraduates Endodontics, with the main objective of this monograph: single session or multi-session Endodontic treatment with intracanalar medication and which are the most effective.