Готові списки джерел за темами / Enteroccus faecalis

Добірка наукової літератури з теми "Enteroccus faecalis"

Автор: Grafiati
Опубліковано: 14 березня 2023

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Статті в журналах з теми "Enteroccus faecalis"

1

Sietho, Randy Carlos, Mandojo Rukmo, Edhie Arif Prasetyo та Tamara Yuanita. "EKSPRESI TNF-α DAN CALCINEURIN PADA ASIMTOMATIS APIKAL PERIODONTITIS AKIBAT INDUKSI Enterococcus faecalis". Conservative Dentistry Journal 7, № 2 (5 грудня 2019): 74. http://dx.doi.org/10.20473/cdj.v7i2.2017.74-85.

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Анотація:
Background. Gram positive bacteria strain are the major cause of endodontic failure as asymptomatic apical periodontitis. One of the dominant group of bacteria is Enterococcus faecalis that still persistent in root canal system post endodontic therapy procedures. This bacteria has lipoteichoic acid on its membrane that can cause induction of cytokines expression such as Tumor Necrosing Factor-α (TNF-α) and Calcineurin Purpose. This experiment to demonstrated asymptomatic apical periodontitis that induced with Enteroccus faecalis produce raising amount of TNF-α and Calcineurin expression cells in pericapical tissue of wistar rat. Method. The upper right molar teeth of the rat was drilled until perforation then exposed by BHIB 10µl (control positive group), E.faecalis 106 CFU in BHIB 10µl (experimental group) and without drilling (control negative group) then observed until 21th days and counting the amount of TNF-α and Calcineurin expression cells. Conclusion.The results show that asymtomatic apical periodontitis that was induced E.faecalis produce increasing amount of TNF-α and Calcineurin expression cells in periapical tissue wistar rat.
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2

Baca, Pilar, María Luisa Mendoza-Llamas, María Teresa Arias-Moliz, María Paloma González-Rodríguez, and Carmen María Ferrer-Luque. "Residual Effectiveness of Final Irrigation Regimens on Enteroccus faecalis–infected Root Canals." Journal of Endodontics 37, no. 8 (August 2011): 1121–23. http://dx.doi.org/10.1016/j.joen.2011.05.003.

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3

FREITAS, A. CRISTINA, CÉLIA PAIS, F. XAVIER MALCATA, and TIM A. HOGG. "Microbiological Characterization of Picante da Beira Baixa Cheese." Journal of Food Protection 59, no. 2 (February 1, 1996): 155–60. http://dx.doi.org/10.4315/0362-028x-59.2.155.

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Анотація:
Ninety cheeses of the type Picante da Beira Baixa (a hard, highly salted, and spicy traditional cheese produced in Portugal from a mixture of goat's and ewe's milks) were manufactured and analyzed for microbiological quality at different stages of the 180-day ripening period. The most abundant species of Enterobacteriaceae, staphylococci, enterococci, lactobacilli, and yeasts found in the Picante cheese were Hafnia alvei and Serratia liquefaciens; Staphylococcus hominis and S.xylosus; Enteroccus faecium, E. faecalis, and E. durans; Lactobacillus plantarum and L. paracasei; and Debaryomyces hansenii and Yarrowia lipolytica.
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4

Athanassiadis, B., PV Abbott, N. George, and LJ Walsh. "An in vitro study of the antimicrobial activity of some endodontic medicaments against Enteroccus faecalis biofilms." Australian Dental Journal 55, no. 2 (June 2010): 150–55. http://dx.doi.org/10.1111/j.1834-7819.2010.01222.x.

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5

Barcelona-Andrés, Belén, Alberto Marina, and Vicente Rubio. "Gene Structure, Organization, Expression, and Potential Regulatory Mechanisms of Arginine Catabolism in Enterococcus faecalis." Journal of Bacteriology 184, no. 22 (November 15, 2002): 6289–300. http://dx.doi.org/10.1128/jb.184.22.6289-6300.2002.

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Анотація:
ABSTRACT Although Enteroccus faecalis is the paradigm for biochemical studies on the arginine deiminase (ADI) pathway of fermentative arginine catabolism, little genetic information exists on this pathway in this organism. We fill this important gap by characterizing, in an 8,228-bp region cloned from a λgt11 genomic library of E. faecalis, a five-gene cluster forming a transcriptional unit (revealed by Northern blots and primer extension in E. faecalis) that corresponds to the ADI operon. Four additional genes in the opposite DNA strand and one in the same DNA strand are also identified. Studies on the protein products, including heterologous expression and/or sequence comparisons, allow us to ascertain or propose functions for all but 1 of the 10 genes. The ADI operon genes, arcABCRD, encode, respectively, ADI, ornithine transcarbamylase, carbamate kinase, a putative Crp/Fnr-type regulator (ArcR), and a putative ornithine-arginine antiporter (ArcD). Arginine induces the expression of arcABCRD, most likely by means of two homologous ArgR/AhrC-type regulators encoded by two genes, argR1 and argR2, that precede arcABCRD in each DNA strand and that are transcribed monocistronically, their transcription being influenced differentially by glucose and arginine. Potential ArgR1/ArgR2 (double and single) binding sequences are found in the promoter regions of arcA and of argR1/argR2 themselves. In addition, putative binding sequences for ArcR and for CcpA are found, respectively, in the argR1/argR2 and arcA promoter regions. Of the three other genes identified, two form a transcriptional unit and encode a putative metal-sensitive transcriptional regulator (ArsR) and a cysteine protease.
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6

Barbaccia, Pietro, Leopoldo Lipocelli, Giancarlo Moschetti, Nicola Francesca, Simone De Martino, Vincenzo Arrigo, Raimondo Gaglio, and Luca Settanni. "Application of Hydrogen Peroxide to Improve the Microbiological Stability of Food Ice Produced in Industrial Facilities." Applied Sciences 12, no. 1 (December 26, 2021): 210. http://dx.doi.org/10.3390/app12010210.

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Анотація:
This work was aimed to produce an “active” food ice to preserve its microbiological safety over time. With this in mind, ice cubes were processed with the addition of H2O2 to water before freezing. Four food ice productions were performed at the industrial level: one control trial without the addition of H2O2 (0OX) and three experimental trials obtained by adding 4, 8, and 12 mg/L of H2O2 (4OX, 8OX, and 12OX), respectively. After production, all food ice trials were artificially contaminated with 102 CFU/100 mL of water-borne pathogenic bacteria (Escherichia coli ATCC 25922, Enteroccus faecalis ATCC 29212, and Pseudomonas aeruginosa ATCC 27853) inoculated individually. Thawed ice samples were then subjected to microbiological analyses performed by the membrane filtration method and the results indicated that only trial 12OX was able to inactivate all bacteria strains. In conclusion, the addition of 12 mg/L H2O2 represents an optimal cost-effective strategy to preserve the microbiological stability of food ice even when it is improperly handled after production.
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7

Porras, M., S. Madrigal, and J. Vega. "Síntesis de Nanoparticulas Poliméricas de Quitosano Funcionalizadas con extractos de la mora (Rubus glaucus) y su Evaluación preliminar como agentes antimicrobianos." Revista Científica 22, no. 1 (December 31, 2012): 81–91. http://dx.doi.org/10.54495/rev.cientifica.v22i1.126.

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Анотація:
El objetivo de este trabajo ha sido evaluar el efecto bacteriostático de nanopartículas de quitosano funcionalizadas con agentes antioxidantes de extractos de frutas tropicales. En la síntesis y caracterización de nanopartículas de quitosano funcionalizadas se manipulan variables experimentales para obtener concentraciones y otros parámetros que presenten efecto bacteriostático en seis especies de bacterias patógenas ATCC, tanto gram-negativas: Escherichia coli, Serratia marcescens y Psudomona aeroginosa; como gram-positivas: Staphylococcus aureus, Bacillus subtilis y Enteroccus faecalis.La hipótesis planteada supone que al funcionalizar las nanopartículas sintetizadas mediante el método de gelificación iónica con tripolifosfato de sodio como agente entrecruzante, con agentes activos provenientes de extractos de la fruta tropical mora (Rubus glaucus), al contener agentes antioxidantes, expresados como concentración de acido gálico, otorgan a las nanopartículas de quitosano la capacidad de inhibir el crecimiento bacteriano. Este trabajo se ha concentrado en el estudio de las propiedades de la mora como agente modificante de las nanopartículas de quitosano y la evaluación preliminar de su actividad antimicrobiana. Se evaluó el contenido de polifenoles de la fruta y su actividad antimicrobiana en conjunto con las nanopartículas frente a los microorganismos patógenos antes mencionados. Las frutas evaluadas presentan contenidos de compuestos fenólicos de aproximadamente 100 mg de ácido gálico/100g y actividad antimicrobiana frente a las bacterias gram-positivas S. aureus, E. faecalis, y B. subtilis y la gram-negativa S. marcescens, determinada por porcentajes de inhibición de un ámbito entre 10% y 60% con respecto al control positivo. Estos resultados muestran que estos nanoproductos tienen potencial como posibles agentes antimicrobianos para ser utilizados en la industria farmaceútica.
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Lauková, A., V. Strompfová, R. Szabóová, A. Slottová, M. Tomáška, V. Kmeť, and M. Kološta. "Bioactive Enterococci Isolated from Slovak Ewes’ Lump Cheese." Scientia Agriculturae Bohemica 47, no. 4 (December 1, 2016): 187–93. http://dx.doi.org/10.1515/sab-2016-0027.

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Анотація:
Abstract Enterococci are widespread organisms; some of their properties are desired in dairy industry. They can produce antimicrobial proteinaceous substances (enterocins) linked to food biopreservation. This study focused on bioactive Enterococcus faecium and Enterococcus faecalis strains from Slovak ewes’ lump cheeses to check genes encoding enterocins production and inhibition activity. The total counts of enterococci in ewes’ lump cheeses reached 5.95 ± 2.44 log CFU/g on average. Genotypization by PCR and identification by MALDI-TOF mass spectrometry alloted 12 strains to the species Enterococcus faecium and 18 strains to the species E. faecalis. Enterococci were hemolytic phenotype free. Gelatinase negative strains were tested for the presence of enterocins genes. E. faecium and E. faecalis strains from Slovak ewes’ lump cheeses possessed mostly genes for enterocins P and A. Enterocin gene free E. faecalis EE29E3 inhibited indicator Enterococcus avium EA5 (inhibition zone > 10 mm); EE36E1inhibited Listeria innocua LMG 13568 (inhibiton zone 12 mm). Among E. faecium possessing enerocins genes, inhibition activity was only noted in EF27E4 strain (against E. avium EA5, Listeria monocytogenes CCM4699; inhibiton zone 10–22 mm). E. faecium EF27E4 was selected for more detailed studies in vitro aimed at its potential use in dairy industry.
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Toc, Dan Alexandru, Stanca Lucia Pandrea, Alexandru Botan, Razvan Marian Mihaila, Carmen Anca Costache, Ioana Alina Colosi, and Lia Monica Junie. "Enterococcus raffinosus, Enterococcus durans and Enterococcus avium Isolated from a Tertiary Care Hospital in Romania—Retrospective Study and Brief Review." Biology 11, no. 4 (April 14, 2022): 598. http://dx.doi.org/10.3390/biology11040598.

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Анотація:
(1) Background: This paper aims to provide a description of non-faecalis non-faecium enterococci isolated from a tertiary care hospital in Romania and to briefly review the existing literature regarding the involvement of Enterococcus raffinosus, Enterococcus durans and Enterococcus avium in human infections and their antimicrobial resistance patterns; (2) Methods: We retrospectively analyzed all Enteroccocus species isolated from the “Prof. Dr. O. Fodor” Regional Institute of Gastroenterology and Hepatology from Cluj-Napoca during one year focusing on non-faecalis non-faecium Enterococci. A brief review of the literature was performed using case reports involving Enterococcus raffinosus, Enterococcus durans and Enterococcus avium; (3) Results: Only 58 out of 658 Enteroccocus isolates were non-faecalis non-faecium and met the inclusion criteria. These species were isolated more often (p < 0.05) from the surgical ward from mixed etiology infections with E. coli. In our review, we included 39 case reports involving E. raffinosus, E. durans and E. avium; (4) Conclusions: Isolation of non-faecalis non-faecium enterococci displays an emerging trend with crucial healthcare consequences. Based on the analysis of the case reports, E. avium seems to be involved more often in neurological infections, E. durans in endocarditis, while E. raffinosus displays a more heterogenous distribution.
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10

Odeyemi, Adebowale, and Paul Omorovie. "Pathogenicity and antibiotic resistance of Enterococcus faecalis isolated from water used in health-care centers of Ekiti State University and environ." International Journal of Biological Research 4, no. 2 (September 26, 2016): 220. http://dx.doi.org/10.14419/ijbr.v4i2.6636.

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Анотація:
The quality of water samples obtained from the health-care center in the Ekiti State University and three other centers around the campus; Ekiti State University Teaching Hospital (EKSUTH), Iworoko-Ekiti health Centre (IHC) and the State Hospital, Ikole-Ekiti (SHI) were investigated by analyzing the total bacterial count using pour plate method; the incidence and antibiotic resistance of Enterococcus faecalis as water quality indicator was enumerated using selective isolation and disk diffusion method respectively. The mean TBC, TCC and TEC of all the water samples ranged from 9.1 x 102 to 17.4 x 103 CFU/ml, 4.1 x 102 to 5.5 x 103 CFU/ml and 0.4 x 102 to 0.4 x 103 CFU/ml respectively. A total of 70 (32.9%) Enterococcus faecalis were recovered from the water samples from Iworoko HC, which showed highest distribution in bore-hole and well water samples while least frequency of E. faecalis (15.7%) was recovered from EKSU HC. However, no incidence of E. faecalis in table water obtained from all the health-care facilities. Just 35% of 20 selected E. faecalis were caseinase producers while 80% of the isolates were biofilm producers. All the isolates were resistant to cefuroxime, cefixime, augmentin and ceftazidine while only 10% of them were resistant to ofloxacin. 58.6% of the isolates showed MAR to eight (8) antibiotics with three different resistotypes while only 1.4% of them showed MAR to four (4) antibiotics with just one resistotype (CRX-CXM-AUG-CAZ). Only E. faecalis15 among the selected isolates possessed two plasmids with molecular weight of 1.415bp and 13.535bp. However, consumption of contaminated water traceable to faecal sources and plasmid mediated of the causative microbes would be discussed.
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Більше джерел

Дисертації з теми "Enteroccus faecalis"

1

De, Giorgi Stefano. "Bacterial infections of the genital tract of infertile couples and in vitro fertilization." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1138555.

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Анотація:
Infertility is estimated to affect approximately 9-12% of reproductive aged couples worldwide. The causes of infertility can be attributed to several pathological conditions affecting one or both partners. The introduction of “Assisted Reproduction Technologies” (ART) has allowed remarkable scientific and medical advances in the field of assisted reproduction. ART consists of different strategies to overcome some infertility factors, thereby improving reproduction efficacy. Infections of the urogenital tract may contribute to infertility with different sexually transmitted diseases (STDs) being directly or indirectly associated to infertility. Among the pathogens that have been associated to infertility, there is Enterococcus faecalis. This microorganism, previously considered just as a member of the gut microbiota of both animals and humans, is now acknowledged as an important human pathogen responsible for a variety of infections, including infections of the urogenital tract, but also life-threating infections such as sepsis and endocarditis. The presence of acquired resistance to major antibiotic classes, in addition to natural intrinsic resistance, is a relevant issue for the treatment of enterococcal infections. Aminoglycosides in combination with β-lactams is the frontline drug combination therapy for severe enterococcal infections. Of special concern is the acquisition, by horizontal transfer, of genes coding for aminoglycoside modifying enzymes (AMEs) which confer resistance to high levels of aminoglycosides (HLA) and eliminate the synergistic bactericidal activity with β-lactams. Despite genital tract infections can affect human fertility, there are no consensus guidelines available on their management (i.e., microbial screening, antibiotic treatment, therapy outcome) in infertile couples undergoing ART treatment. In the present thesis, an attempt was made to better understand how infections impact on couple fertility. We have also explored the effect of hormone therapy on vaginal microbiota and reproductive outcomes of females undergoing in vitro fertilization (IVF). In addition, we have characterized the antibiotic susceptibility and population structure of a collection of E. faecalis clinical strains isolated from the genital tract of infertile couples to provide clinicians with relevant data to implement management of urogenital infections in infertile couples. Chapter 2 is a general introduction to infertility-associated infections with a special focus on E. faecalis. In the first section, virulence determinants, disease pathogenesis and clinical manifestations of E. faecalis are described. Then, treatment of enterococcal infections with a broad overview on action and resistance mechanisms of major antibiotic classes is provided. Chapter 3 explores the role of urogenital infections on couple fertility starting from a collection of vaginal/endocervical swabs and semen samples from 285 infertile couples. The impact of different bacterial species on the outcome of IVF was examined. The results showed the negative impact of E. faecalis on sperm quality and the association of different bacterial pathogens with reduced levels of vaginal lactobacilli. Interestingly, the presence of E. faecalis together with Ureaplasma urealyticum/Mycoplasma hominis in genital samples of infertile couples was predictive for a negative outcome of IVF. Chapter 4 describes the phenotypic and genotypic features of 41 “infertility-associated E. faecalis” (IAF) strains described in chapter 3. Antibiotic susceptibility of different drugs was carried out (Vitek, MIC and disk diffusion methods) and assessed using EUCAST guidelines. The majority of IAF isolates were susceptible to clinically relevant antibiotics, except for 8 strains that were resistant to HLA and 1 which was also resistant to fluoroquinolones. In order to characterize the IAF isolates, whole genome sequences were used to get insights into the IAF population structure and analyse the genetic bases of antimicrobial resistance. Multi-locus sequence typing (MLST) showed a high diversity of the IAF population. However, a clonal structure of HLA resistant strains was found, as 6 out of 8 resistant IAF isolates belonged to the same clonal complex (CC)/sequence type (ST) CC16/ST480. The work described in chapter 5 evaluates the effect of treatment with exogenous gonadotropins on the vaginal microbiota of 108 infertile women undergoing controlled ovarian stimulation prior to being subjected to IVF. A significant increase of vaginal diamines was observed following hormonal treatment. Analysis of vaginal swabs revealed that a shift occurred after hormone treatment from a Lactobacillus-based microbiota to a microbial population mostly constituted by streptococci, enterococci, enterobacteria, staphylococci and yeasts. A highly significant association between reduced amounts of vaginal lactobacilli and the presence of above mentioned pathogens was found. Finally, IVF outcome was significantly decreased in the patients whose vaginal samples were positive for high levels of diamines and presence of bacterial pathogens, suggesting that a link may exist between vaginal microbiota dysbiosis due to hormonal treatment and IVF failure. Chapter 6 contains a short paragraph with the main conclusions of this Ph.D. thesis.
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2

Sumi, Jáuregui Ada Elizabeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Universidad Nacional Mayor de San Marcos. Programa Cybertesis PERÚ, 2008. http://www.cybertesis.edu.pe/sisbib/2008/sumi_ja/html/index-frames.html.

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Анотація:
El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes
The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments
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3

Sumi, Jáuregui Ada Lizbeth. "Transferencia de plásmidos con resistencia a antibióticos en especies de Enterococcus provenientes del mar de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/907.

Повний текст джерела
Анотація:
El género Enterococcus es conocido por ser de origen fecal o intestinal, pero tiene una amplia distribución en la naturaleza y se le puede encontrar en suelos, aguas, plantas y en productos alimenticios, siendo capaz de sobrevivir en medios poco enriquecidos. Los estudios reportados sobre estos microorganismos generalmente inciden en su aspecto clínico y su resistencia a antibióticos, y algunos se ubican en un contexto ambiental evaluando métodos para su detección o enumeración para uso en aguas recreacionales. Está aumentando la importancia de este microorganismo como agente causal de infecciones adquiridas en hospitales, pero el interés de estudio en este género radica en su alta resistencia natural a múltiples antimicrobianos y a su capacidad de adquirir y transferir dicha resistencia. Se sabe que Enterococcus es un microorganismo introducido al ecosistema marino debido a la contaminación de éste ambiente con desechos orgánicos, pero son pocos los reportes sobre estudios de resistencia antimicrobiana de éste género provenientes de muestras de agua de mar, siendo necesario este tipo de investigación que nos permita conocer la importancia de estos microorganismos en estos ambientes.
The genus Enterococcus is recognized as being of fecal origin but have a wide distribution in nature, they can be found in soil, water, plants and food products, being able to survive in low-enriched media. Studies on these microorganisms usually affect their appearance and clinical resistance to antibiotics, and there are some who are placed in an environmental context, evaluating methods of detection or enumeration in waters for recreational use. It is increasing the importance of this microorganism as a causative agent of infections acquired in hospitals, but the interest in this kind of study lies in its high natural resistance to multiple antimicrobials and their ability to acquire and transfer the resistance. Despite that Enterococcus is a microorganism introduced to the marine ecosystem by contamination with organic wastes, there are few reports on studies of antimicrobial resistance of the Enterococcus genus water samples from the sea, being necessary to this type of research that allows us to know the importance of these microorganisms in these environments.
Tesis
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4

Roberts, Gretta. "Glycoprotein utilisation by enterococcus faecalis." Thesis, King's College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412446.

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5

Barros, Mariana Vilhena. "Infeções nosocomiais por enterococcus faecalis." Master's thesis, [s.n.], 2014. http://hdl.handle.net/10284/4512.

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Анотація:
Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas
As infeções nosocomiais são consideradas um problema mundial de saúde pública. A sua disseminação tem contribuído para aumento das taxas de mortalidade e morbilidade, a maioria das vezes devido às limitadas ou mesmo inexistentes opções terapêuticas. Enterococcus faecalis é uma bactéria Gram-positiva, anaeróbia facultativa, presente na flora comensal do trato gastrintestinal de humanos e animais. Apesar da sua suposta inocuidade, nas últimas décadas E.faecalis tem-se revelado um patogénio oportunista, representando a segunda e a terceira maior causa de infeções hospitalares a nível mundial. Esta bactéria é apontada como uma das principais causas de endocardites, bacteremias, infeções do trato urinário, intra-abdominais e de feridas contraídas em hospitais. As suas caraterísticas fisiológicas permitem-lhe sobreviver a altas temperaturas, a elevados valores de pH e concentrações salinas. Esta bactéria resiste também em ambientes hostis, em situações de subnutrição, de stress oxidativo e às técnicas tradicionais de limpeza. Apresenta inúmeros fatores de virulência nomeadamente proteínas de superfície, enzimas hidrolíticas e capacidade de formação de biofilmes, o que auxilia esta bactéria a invadir, colonizar e infetar tecidos hospedeiros. Enterococcus faecalis exibe uma resistência intrínseca a algumas classes de antibióticos como β-lactâmicos, lincosamidas, trimetropim-sulfametoxazol, fluoroquinolonas e baixas concentrações de aminoglícosídeos. Devido à sua capacidade mutagénica e adaptativa, este microrganismo desenvolve e adquire novas resistências, através de mutações cromossomais ou por transferência de genes. Como é o caso do gene vanA e vanB, que lhe conferem resistência à vancomicina (VRE). E.faecalis tem-se revelado uma constante ameaça de vida em todo o mundo, por isso é urgente controlar a disseminação desta bactéria. É importante desenvolver novos métodos terapêuticos, tendo em conta a ineficácia dos atuais e aplicar estratégias, a nível hospitalar, que diminuam ao máximo a transmissão do microrganismo. The nosocomial infections are nowadays a worldwide issue in terms of health. Its dissemination has been the cause of the rising of mortality and disease rates, most of the time due to limited or even nonexistent therapeutic options. Enterococcus faecalis is a Gram-positive anaerobic facultative bacterium witch is found in the flora of the gastrointestinal tract of humans and animals. Despite its alleged harmlessness, lately E.faecalis has been proved to be an opportunistic pathogen, representing the second and the third leading cause of hospital-acquired infections worldwide. This bacterium is being pointed as a mainly cause of endocarditis, fast progressing bacteremia may present, urinary tract infections, intra-abdominal and wounds contracted in hospitals. Its physiological characteristics allows this bacterium to survive at high temperatures, high PH values and highly concentration salts. It can also resist in hostiles environments such as malnutrition, oxidative stress and traditional cleaning technics. It feature numerous virulence factors including surface proteins, hydrolytic enzymes and ability of biofilm formation, which helps the bacterium to invade, colonize and infect the host tissue. Enterococcus faecalis displays an intrinsic resistance to some classes of antibiotics such as β-lactam, lincosamides, trimetropim-sulfamethoxazole, fluoroquinolones and low concentration of aminoglycosides. Due to its mutagenic capacity and adaptive skills, this micro-organism develops and acquires new resistances, through chromosome mutations or by genetic transferences e.g. gene vanA e vanB, witch confers resistance to Vancomycin (VRE). E. faecalis has proved a constant threat of life throughout the world, so there is an urgent need to control the spread of this bacteria by developing new therapeutic methods, having regard to the ineffectiveness of current ones and looking for new strategies inside hospitals that will reduce as much as possible the transmission of the micro-organism.
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Böttcher, Daiana Elisabeth. "Avaliação do efeito da presença do biofilme de Enterococcus faecalis no canal radicular sobre a manutenção da substantividade da clorexidina a 2% : estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/110768.

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Анотація:
Introdução: O objetivo do presente estudo foi correlacionar o efeito antimicrobiano residual e a substantividade da solução de clorexidina a 2%, em dentina humana de dentes extraídos e contaminada com E. faecalis, por 48 horas, 7 e 30 dias. Metodologia: Cento e vinte e três dentes humanos extraídos foram utilizados para esse estudo. As amostras foram divididas em quatro grupos conforme a solução irrigadora utilizada (CHX 2% ou soro fisiológico) e na presença ou ausência do biofilme de Enterococcus faecalis. As amostras foram mantidas em contato com a respectiva solução durante 5 minutos. Cada grupo foi distribuído aleatoriamente em 3 subgrupos de acordo com o período de avaliação (n=10). A quantidade de CHX presente foi avaliada através de cromatografia líquida de alta eficiência (HPLC) e a viabilidade bacteriana foi analisada através de microscópio confocal a laser (CLSM). A análise estatistica foi feita através dos testes de Kruskal-Wallis e Mann-Whitney U (P<.05) e teste de correlação de Spearman (P<.01). Resultados: Houve uma correlação negativa entre o percentual de células viáveis e a quantidade de CHX remanescente (P = .000). A CHX reduziu significativamente o percentual de células viáveis em relação ao soro após 48 horas (P = .007). A diferença foi mantida no período de 7 dias (P = .001). Após 30 dias, o grupo CHX apresentou um aumento da viabilidade bacteriana tornando-se semelhante ao soro (P = .623). Simultaneamente, a quantidade de CHX reduziu significativamente após 30 dias (P = .000). Conclusão: Os resultados do presente estudo indicam que a CHX a 2% foi detectada nos periodos de 48 horas e 7 dias, mantendo reduzido o percentual de células viáveis. A presença de microrganismos na dentina humana não alterou a quantidade de CHX residual.
Introduction: The aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (CHX) on dentin by means of confocal laser scanning microscope (CLSM) and high-performance liquid chromatography (HPLC) for 48 hours, 7 and 30 days. Methods: One hundred twenty three extracted human teeth were used. Samples were divided into 4 groups according to the solution (CHX or saline) and the presence of Enterococus faecalis biofilm. Samples were kept in contact with 5mL of the solution for 5 minutes. Each group was divided into 3 subgroups according to the evaluation period (n = 10). Statistical analysis was performed by using the Kruskal- Wallis, Mann-Whitney U tests (P < .05) and Spearman’s Rank Correlation Coefficient (P < .01). Results: There was a negative correlation between the percentage of live cells and the amount of remaining CHX (P = .000). CHX significantly reduced the percentage of viable cells compared to saline after 48 hours (P = .007). Differences were maintained in the 7-day evaluation (P = .001). After 30 days, CHX group presented an increase of viable cells, thereby becoming similar to saline (P = .623). Simultaneously, remaining CHX significantly reduced in the 30-day specimens (P = .000). Conclusion: The results of this study indicate that 2% CHX solution was detected for 48 hours and 7 days, keeping a low percentage of viable cells. The presence of microorganisms on human dentin did not affect 2% chlorhexidine maintenance.
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Tse, Chee-choong Micheal, and 謝志聰. "Effect of ultrasonic agitation on enterococcus faecalis biofilm." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45165993.

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Abadía, Patiño Lorena. "Caractérisation de l'opéron vanE chez Enterococcus faecalis BM4405." Paris 7, 2003. http://www.theses.fr/2003PA077001.

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Diederich, Ann-Kristin [Verfasser], and Johannes [Akademischer Betreuer] Hübner. "Membrane lipids of Enterococcus faecalis as microbial pathogens." Freiburg : Universität, 2016. http://d-nb.info/1119452708/34.

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Neto, Manoel Matos. "Avaliação in vitro da eficácia de técnicas endodônticas de preparo mecânico na redução de Enterococcus faecalis." Universidade de Taubaté, 2007. http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=483.

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O preparo mecânico tem por objetivo a modelagem dos canais radiculares e redução de microrganismos nele presentes. Para sua execução foram utilizados instrumentos endodônticos confeccionados em aço inoxidável ou ligas de níquel-titânio. Esse estudo tem como objetivo avaliar a eficácia de três sistemas de preparo mecânico na redução da contagem de Enterococcus faecalis no interior de canais radiculares. Para isso foram utilizados 24 dentes humanos unirradiculares, divididos em três grupos: G1 - instrumentação com o sistema ProTaper rotatório, G2 - com sistema ProTaper manual e G3 - com limas tipo Kerr manuais. Os canais radiculares foram contaminados com Enterococcus faecalis, e após formação de biofilme, submetidas ao preparo mecânico. As unidades formadoras de colônia foram contadas antes e depois da instrumentação e a porcentagem de redução calculada. Os resultados demonstraram que todos os sistemas reduziram significantemente a quantidade de microrganismos dentro do canal radicular. Contudo, quando se comparou a redução promovida pelos sistemas entre si não houve diferença significativa. O estudo confirmou a eficácia do preparo mecânico, entretanto nenhum dos sistemas avaliados foi superior ao outro na redução do número de Enterococcus faecalis no interior dos canais radiculares.
The objects of mechanical preparation are to model root canals and reduce the number of microorganisms present in them. For this purpose, endodontic instruments made of stainless steel or nickel-titanium alloys are used. The aim of this study was to assess the efficacy of three systems of mechanical preparation for reducing the counting of Enterococcus faecalis inside root canals. To do this, 24 single rooted human teeth were used, divided into three groups: G1 instrumentation with the ProTaper rotary system, G2 with the manual ProTaper system and G3 manual Kerr type files. The root canals were contaminated with Enterococcus faecalis, and after biofilm had formed, they were submitted to mechanical preparation. The colony forming units were counted before and after instrumentation and the percentage of reduction was calculated. The results demonstrated that all the systems significantly reduced the number of microorganisms inside the root canal. However, when the reduction promoted by the systems was compared among them, there was no significant difference. The study confirmed the efficacy of mechanical preparation, however, none of the assessed systems was superior to the other for reducing the number of microorganisms inside root canals.
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Книги з теми "Enteroccus faecalis"

1

Berthold, Martin. Die Pathogenität biofilmassoziierter Virulenzfaktoren von Enterococcus faecalis im Endokarditismodell. [S.l: s.n.], 2014.

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2

Cattelan, Jessy. Immunologische Studie zum Oberflächenprotein TraM, ein Typ IV Sekretionsprotein in Enterococcus faecalis / Enterococcus faecium. [S.l: s.n.], 2014.

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3

Shorrock, Patricia Joan. Surface properties of enterococcus faecalis in relation to infective endocarditis. Birmingham: Aston University. Department of Pharmaceutical Sciences, 1990.

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4

Bao, Yinyin. Role of mprF1 and mprF2 in the pathogenicity of Enterococcus faecalis. Freiburg: Universität, 2012.

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5

Peters, Tansy M. Studies on the activation of azo-dyes into direct-acting genotoxic agents by enterococcus faecalis. London: University of East London, 1995.

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6

Mack, Henry L. Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects. Nova Science Publishers, Incorporated, 2014.

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7

Bambawale, Amrut, Sumita Bhagwat, and Leena Padhye. Aktywność przeciwdrobnoustrojowa Propolis, HEBP, chlorheksydyny, EDTA: Przeciwko Enterococcus Faecalis i Candida Albicans ; badanie Invitro. Wydawnictwo Nasza Wiedza, 2020.

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8

Shepard, Brett David. Differential expression of virulence-related genes by Enterococcus faecalis in response to biological cues. [s.n.], 2002.

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9

Herrington, William G., Aron Chakera, and Christopher A. O’Callaghan. Urinary tract infection. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0158.

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Анотація:
A urinary tract infection (UTI) is defined as present when urine culture results in the growth of a single organism at greater than 105 colony-forming units/ml of urine. Bowel flora are the commonest cause of UTIs. Escherichia coli accounts for 80% of infections. Klebsiella spp., Proteus mirabilis, Enterococcus faecalis, and Staphylococcal saprophyticus account for most of the remaining 20%. Staphylcoccus aureus culture is usually catheter related or secondary to haematogenous spread. This chapter looks at the symptoms of a UTI, as well as its demographics, complications, diagnosis (including investigations), and treatment. It also discusses prognosis.
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10

Hancock, Lynn Ernest. Identification and characterization of an enterococcus faecalis capsular polysaccharide genetic determinant and the contribution of the capsular polysaccharide to the biology of the organism. [s.n.], 2001.

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Частини книг з теми "Enteroccus faecalis"

1

Murray, Barbara E., Liangxia Jiang, Jianguo Xiao, Xiang Qin, Kavindra V. Singh, Aart de Kok, Al Claiborne, Patrice Courvalin, and George M. Weinstock. "Enterococcus faecalis." In Bacterial Genomes, 649–50. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_59.

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2

Manson, Janet M., and Michael S. Gilmore. "Pathogenomics of Enterococcus faecalis." In Pathogenomics, 125–47. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2006. http://dx.doi.org/10.1002/352760801x.ch7.

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3

Wirth, Reinhard. "The sex pheromone system of Enterococcus faecalis." In EJB Reviews 1994, 117–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-79502-2_9.

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4

Fujimoto, S., M. Bastos, K. Tanimoto, F. An, K. Wu, and D. B. Clewell. "The pAD1 Sex Pheromone Response in Enterococcus faecalis." In Streptococci and the Host, 1037–40. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_244.

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5

Clermont, Dominique, Gilda de Cespédès, Françoise Delbos, and Théa Horaud. "Genetic Analysis of Enterococcus faecalis Chromosome Carrying Mobile Elements." In Streptococci and the Host, 1023–27. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_241.

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6

Shankar, Viswanathan, and Michael S. Gilmore. "Characterization of the Enterococcus faecalis Alpha C Protein Homolog." In Streptococci and the Host, 1045–48. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_246.

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7

Nakayama, Jiro, Yuuichiro Takanami, and Akinori Suzuki. "Analysis of Pheromone Binding and Pheromone Reception by Enterococcus faecalis." In Streptococci and the Host, 1033–35. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_243.

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8

Pasquarella, Cesira, Donald Morrison, Angelo Savino, and Barry D. Cookson. "Dynamics of Enterococcus faecalis Colonization of Bone Marrow Transplant Patients." In Streptococci and the Host, 275–79. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1825-3_68.

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9

McShan, W. Michael, and Nathan Shankar. "The Genome of Enterococcus faecalis V583: a Tool for Discovery." In The Enterococci, 409–15. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817923.ch11.

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10

Clewell, Don B. "Sex Pheromones and the Plasmid-Encoded Mating Response in Enterococcus faecalis." In Bacterial Conjugation, 349–67. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_14.

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Тези доповідей конференцій з теми "Enteroccus faecalis"

1

Santos, Pâmela Gomes, Rosane Nassar Meireles Guerra, Josivan Regis Farias, Simone Batista Muniz, and Danielle Cristine Gomes Franco. "AÇÃO ANTIMICROBIANA DAS FLORES DE ANACARDIUM OCCIDENTALE E DO ÁCIDO ELÁGICO PRESENTE NO EXTRATO." In I Congresso Brasileiro de Imunologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/965.

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Introdução: As infecções bacterianas têm aumentado significativamente nas últimas décadas, sobretudo aquelas ocasionadas por microrganismos multirresistentes. Assim, o uso de produtos naturais com finalidades terapêuticas surge com alvo de bioprospecção na busca de novos compostos com ação antimicrobiana. Além disso, o uso de insetos, como o Tenebrio molitor como modelo experimental para avaliação in vivo tem sido muito frequente, pois exige menos material em relação aos testes com animais vertebrados. Objetivo: O presente trabalho investigou o efeito citotóxico e ação antimicrobiana do extrato hidroalcoólico das flores de Anacardium occidentale (EHAo) e do ácido elágico. Material e Métodos: Avaliamos a citoxicidade de ácido elágico e do EHAo nas concentrações (1; 5 e 50mg/kg) em Tenebrio molitor. A ação antimicrobiana para Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae e a toxicidade do ácido elágico, foi avaliada por microdiluição, segundo a norma M7-A6 do manual da Clinical and Laboratory Standards Institute – CLSI. Foi determinada a Concentração Bactericida Mínima (CBM) e concentração inibitória mínima (CIM), em culturas de 24 horas, incubadas à 37ºC. Resultados: No ensaio de citotoxidade aguda se verificou que nenhuma das concentrações usados foram tóxicas, pois não ocorreram óbitos e nem nenhuma anormalia morfológica nas larvas de Tenebrio molitor. Os testes de concentração inibitória mínima (CIM) de concentração bactericida mínima (CBM) mostraram que o EHAo apresentou ação bactericida para Enterococcus faecalis em todas as concentrações testadas. Para Staphylococcus aureus os resultados mostraram ação bactericida para as maiores concentrações e bacteriostática para a menor diluição. O ácido elágico teve ação bactericida apenas para Enterococcus faecalis. Para as bactérias Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa e Klebsiella pneumoniae as concentrações de EHAo e ácido elágico testadas não foram inibitórias. Conclusões: Os resultados mostraram baixa toxicidade tanto para o EHAo como para o ácido elágico e ainda, que o extrato apresentou melhor efeito antimicrobiano do que o ácido elágico, para Enterococcus faecalis e Staphylococcus aureus.
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Keyhani, Jacqueline, and Ezzatollah Keyhani. "Increased resistance to detergent in Enterococcus faecalis." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0010.

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Tverezovska, Olesia, Viktoriia Holubnycha, Rafal Banasiuk, Yevheniia Husak, Savchenko Anton, and Viktoriia Korniienko. "The Effect of Silver Nanoparticles Against Formation of Enterococcus Faecalis Biofilms." In 2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP). IEEE, 2022. http://dx.doi.org/10.1109/nap55339.2022.9934155.

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4

Du, Juan, Hofung Cheng, Ken T. M. Wong, and Stanley C. K. Lau. "A microcosm study on the die-off response of the indicator bacteria, Enterococcus faecium and Enterococcus faecalis." In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0001.

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LUIS BAPTISTA DE GUSMÃO, PEDRO, THAIS DA SILVA FEITOSA, Brenda Paula Figueiredo de Almeida Gomes, Eloá Cristina Bícego-Pereira, and MAICON RICARDO ZIEBERG PASSINI. "Isolamento e identificação de Enterococcus faecalis de dentes submetidos à reintervenção endodôntica." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-51716.

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6

Ke Sun, Jue Zhang, Jing Fang, Jing Wang, Jie Pan, and Weidong Zhu. "Cold plasma therapy for enterococcus faecalis biofilm infected tooth root canal in vitro." In 2012 IEEE 39th International Conference on Plasma Sciences (ICOPS). IEEE, 2012. http://dx.doi.org/10.1109/plasma.2012.6383868.

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7

Paula Figueiredo De Almeida Gomes, Brenda, Maikon Tadeu Ferrari Martinucho, Ela͍se Gabriele Martins, and Beatriz Leonardo Prudenciano. "Avaliação de substâncias químicas auxiliares, utilizadas em endodontia, na redução de Enterococcus faecalis." In XXIII Congresso de Iniciação Científica da Unicamp. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.19146/pibic-2015-38003.

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Pinto, Vanessa Arantes, Gabriela Santos Alencar, Adriano Favero, Hugo Franciscon, and Fagner Luiz Da Costa Freitas. "POTENCIAL MICROBIOLÓGICO DO SYZYGIUM AROMATICUM (L). SOBRE MICRORGANISMOS DE IMPORTÂNCIA NA MEDICINA VETERINÁRIA." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1920.

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Introdução: O fenômeno de resistência a antimicrobianos está se mostrando um grande problema na saúde, ameaçando tanto a população humana como animal. Devido a isso, a descoberta de novos compostos com capacidade antimicrobiana torna-se imprescindível, e os fitoterápicos têm se mostrado uma alternativa promissora na terapia complementar. O óleo essencial (OE) de Syzygium aromaticum (Cravo da Índia), apresenta potencial capacidade antibacteriana e antifúngica devido às altas concentrações de Eugenol. Objetivos: Avaliar o potencial antimicrobiano do óleo essencial de Syzygium aromaticum (L). contra microrganismos de importância na Medicina veterinária. Materiais e métodos: Foram testadas as bactérias gram positivas: S. aureus LB 25923, S. aureus B24, S. aureus NP 38, Streptococcus uberis, S. agalactiae, Enterococcus faecalis e S. epidermidis; as gram negativas: Pseudomonas aeruginosa ATCC 27853 e E. coli ATCC 25922; e os fungos: C. tropicalis e C. gattii 179. Utilizou-se a técnica de disco difusão (Kirby-bauer) aplicando 10µL de OE, e posteriormente, para os patógenos que apresentaram o halo inibitório, determinou-se, aplicando 15µL de OE, a concentração inibitória mínima (CIM), concentração bactericida mínima (CBM) e concentração fungicida mínima (CFM) através da microdiluição seriada utilizando leitor de ELISA e corante resazurina. Resultados: Todos os microrganismos apresentaram halo inibitório. Com relação aos valores de CIM e CBM para as bactérias, obteve-se, respectivamente: S. aureus LB25923 (18,75% e 18,75%), S. aureus B 24 (4,94% e 4,94%) S. aureus NP 38 (3,64% e 3,64%) Streptococcus uberis (2,60% e 3,125%) S. agalactiae (5,20 %, 5,20%), Enterococcus faecalis (5,20% e 6,25%), S. epidermidis (3,38% e 4,42%), Pseudomonas aeruginosa ATCC 27853 (2,09% e 2,09%), E. coli ATCC 25922 (10,41% e 10,41%). Os resultados de CIM e CFM obtidos para os fungos foram respectivamente: C. tropicalis (1,82% e 1,82%) e C. gattii 179 (5,27% e 5,27%). Conclusão: O óleo essencial de Syzygium aromaticum possui considerável potencial antimicrobiano “in vitro”, e dessa forma, pode ser considerado uma possível alternativa para as terapias sintéticas de tratamento das doenças infecciosas bacterianas e fúngicas encontradas na rotina clínica.
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Fernandes, Meg da Silva, Luciana Maria Ramires Esper, Dirce Yorika Kabuki, and Arnaldo Yoshiteru Kuaye. "Produção de Moléculas Sinalizadoras de Quorum Sensing por Cepas de Enterococcus faecium e Enterococcus faecalis Isoladas do Processamento de Ricota." In V Simpósio de Bioquímica e Biotecnologia. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/biochem-vsimbbtec-21938.

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Gu, Jie, David Jaramillo, Paul Webster, and Chunqi Jiang. "Study of oxygen concentration dependence of the plasma-induced bactericidal effect against enterococcus faecalis." In 2013 IEEE 40th International Conference on Plasma Sciences (ICOPS). IEEE, 2013. http://dx.doi.org/10.1109/plasma.2013.6635203.

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Звіти організацій з теми "Enteroccus faecalis"

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A. Komnos, Georgios, Antonios Papadopoulos, Efstratios Athanaselis, Theofilos Karachalios, and Sokratis E. Varitimidis. Migrating Periprosthetic Infection from a Total Hip Replacement to a Contralateral Non-Operated Osteoarthritic Knee Joint. Science Repository, January 2023. http://dx.doi.org/10.31487/j.ijscr.2022.03.02.

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Introduction: There is a paucity of published data on whether a treated infected arthroplasty is a risk factor for infection in another, non-operated joint. Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty is a relatively rare entity. Case: We report a case of migration of a pathogen (Enterococcus faecalis) from an infected prosthetic joint (hip) to the contralateral native joint (knee). Identification of the pathogen was made with PCR, by obtaining cultures during the implantation of the primary knee prosthesis. Conclusion: Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty has not been widely reported. Management of such cases is extremely challenging and without clear and established guidelines. Our experience shows that tissue samples should be taken intraoperatively and sent for cultures, so as to exclude contamination in those cases.
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Bezerra, Alexandre Sacchetti, Flavia Altheman Loureiro, Carla Maria Pasquareli Vazquez, Afonso Cesar Polimanti, and Rafi Felicio Bauab Dauar. Empiric Treatment of Foot Infection in Patients with Severe Diabetes. Science Repository, December 2021. http://dx.doi.org/10.31487/j.jicoa.2021.04.04.

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Background: Despite being treated with antibiotics of broad spectrum recommended by International Consensus, severe diabetic patients with lower limb infection do not present a positive clinical evolution during empirical treatment. This study’s bacterial profile was analysed and compared with other worldwide hospital centers. Objective: To confirm the need of an individualized empirical treatment for severe diabetic patients with foot infection. Methods: Retrospective analysis of cultures and antibiograms of severe diabetic patients admitted by foot infection. Results: The results were consistent with the socioeconomic realities of developing countries. Gram-negative bacteria (52,11%) were present in most bone cultures. Results presented a high incidence of Enterococcus faecalis in both gram-positive (21,2%) and polymicrobial (34,7%) samples. Bacterial resistance with the use of ordinary antibiotics in the statistical analysis was high. Conclusion: The community infections should undergo broad spectrum empirical therapy combining amikacin (80,43%) or meropenem (72,00%) with gram-negative and vancomycin (100%) or teicoplanin (90,00%) or linezolid (74,19%) with gram-positive.
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