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Статті в журналах з теми "Ensifer meliloti"

Автор: Grafiati

Опубліковано: 10 березня 2023

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1

Torres, Maria J., Maria I. Rubia, Teodoro de la Peña, José J. Pueyo, Eulogio J. Bedmar, and María J. Delgado. "Genetic basis for denitrification in Ensifer meliloti." BMC Microbiology 14, no. 1 (2014): 142. http://dx.doi.org/10.1186/1471-2180-14-142.

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2

Baxter, Laura, Proyash Roy, Emma Picot, Jess Watts, Alex Jones, Helen Wilkinson, Patrick Schäfer, Miriam Gifford, and Beatriz Lagunas. "Comparative Genomics across Three Ensifer Species Using a New Complete Genome Sequence of the Medicago Symbiont Sinorhizobium (Ensifer) meliloti WSM1022." Microorganisms 9, no. 12 (November 25, 2021): 2428. http://dx.doi.org/10.3390/microorganisms9122428.

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Анотація:

Here, we report an improved and complete genome sequence of Sinorhizobium (Ensifer) meliloti strain WSM1022, a microsymbiont of Medicago species, revealing its tripartite structure. This improved genome sequence was generated combining Illumina and Oxford nanopore sequencing technologies to better understand the symbiotic properties of the bacterium. The 6.75 Mb WSM1022 genome consists of three scaffolds, corresponding to a chromosome (3.70 Mb) and the pSymA (1.38 Mb) and pSymB (1.66 Mb) megaplasmids. The assembly has an average GC content of 62.2% and a mean coverage of 77X. Genome annotation of WSM1022 predicted 6058 protein coding sequences (CDSs), 202 pseudogenes, 9 rRNAs (3 each of 5S, 16S, and 23S), 55 tRNAs, and 4 ncRNAs. We compared the genome of WSM1022 to two other rhizobial strains, closely related Sinorhizobium (Ensifer) meliloti Sm1021 and Sinorhizobium (Ensifer) medicae WSM419. Both WSM1022 and WSM419 species are high-efficiency rhizobial strains when in symbiosis with Medicago truncatula, whereas Sm1021 is ineffective. Our findings report significant genomic differences across the three strains with some similarities between the meliloti strains and some others between the high efficiency strains WSM1022 and WSM419. The addition of this high-quality rhizobial genome sequence in conjunction with comparative analyses will help to unravel the features that make a rhizobial symbiont highly efficient for nitrogen fixation.

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3

Muratova, Anna, Natalya Pozdnyakova, Oleg Makarov, Mikhail Baboshin, Boris Baskunov, Nina Myasoedova, Ludmila Golovleva, and Olga Turkovskaya. "Degradation of phenanthrene by the rhizobacterium Ensifer meliloti." Biodegradation 25, no. 6 (July 23, 2014): 787–95. http://dx.doi.org/10.1007/s10532-014-9699-9.

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4

Sun, Shi-Lei, Tian-Qi Lu, Wen-Long Yang, Jing-Jing Guo, Xue Rui, Shi-Yun Mao, Ling-Yan Zhou, and Yi-Jun Dai. "Characterization of a versatile nitrile hydratase of the neonicotinoid thiacloprid-degrading bacterium Ensifer meliloti CGMCC 7333." RSC Advances 6, no. 19 (2016): 15501–8. http://dx.doi.org/10.1039/c5ra27966f.

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Анотація:

The nitrogen-fixing bacterium Ensifer meliloti CGMCC 7333 and its nitrile hydratase (NHase) degrade the neonicotinoid insecticides, thiacloprid (THI) and acetamiprid (ACE), to their corresponding amide metabolites.

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5

MAREK-KOZACZUK, MONIKA, JERZY WIELBO, ANNA PAWLIK, and ANNA SKORUPSKA. "Nodulation competitiveness of Ensifer meliloti alfalfa Nodule Isolates and Their Potential for Application as Inoculants." Polish Journal of Microbiology 63, no. 4 (2014): 357–86. http://dx.doi.org/10.33073/pjm-2014-051.

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Анотація:

Alfalfa (Medicago sativa) is a widely cultivated legume, which enters into nitrogen-fixing symbiosis with Ensifer (Sinorhizobium) spp. In this study, an autochthonous rhizobial population of Ensifer sp. occupying alfalfa nodules grown in arable soil was used as the basis for selection of potential inoculants. Alfalfa nodule isolates were identified as Ensifer meliloti by partial 16S rDNA, recA, atpD and nodC nucleotide sequencing. The sampled isolates displayed different symbiotic performance and diversity in the number of plasmids and molecular weight. Isolates that were the most efficient in symbiotic nitrogen fixation were tagged with a constitutively expressed gusA gene carried by a stable plasmid vector pJBA21Tc and used in competition experiments in soil under greenhouse conditions. Two E. meliloti strains LU09 and LU12, which effectively competed with indigenous soil rhizobia, were selected. The metabolic profiles of these selected strains showed differences in the use of carbon and energy sources. In addition, the LU09 strain exhibited bacteriocin production and LU12 mineral phosphate solubilization, which are valuable traits for soil survival. These strains may be considered as potential biofertilizers for alfalfa cultivation.

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6

Biondi, Emanuele G., Enrico Tatti, Diego Comparini, Elisa Giuntini, Stefano Mocali, Luciana Giovannetti, Marco Bazzicalupo, Alessio Mengoni, and Carlo Viti. "Metabolic Capacity of Sinorhizobium (Ensifer) meliloti Strains as Determined by Phenotype MicroArray Analysis." Applied and Environmental Microbiology 75, no. 16 (June 26, 2009): 5396–404. http://dx.doi.org/10.1128/aem.00196-09.

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Анотація:

ABSTRACT Sinorhizobium meliloti is a soil bacterium that fixes atmospheric nitrogen in plant roots. The high genetic diversity of its natural populations has been the subject of extensive analysis. Recent genomic studies of several isolates revealed a high content of variable genes, suggesting a correspondingly large phenotypic differentiation among strains of S. meliloti. Here, using the Phenotype MicroArray (PM) system, hundreds of different growth conditions were tested in order to compare the metabolic capabilities of the laboratory reference strain Rm1021 with those of four natural S. meliloti isolates previously analyzed by comparative genomic hybridization (CGH). The results of PM analysis showed that most phenotypic differences involved carbon source utilization and tolerance to osmolytes and pH, while fewer differences were scored for nitrogen, phosphorus, and sulfur source utilization. Only the variability of the tested strain in tolerance to sodium nitrite and ammonium sulfate of pH 8 was hypothesized to be associated with the genetic polymorphisms detected by CGH analysis. Colony and cell morphologies and the ability to nodulate Medicago truncatula plants were also compared, revealing further phenotypic diversity. Overall, our results suggest that the study of functional (phenotypic) variability of S. meliloti populations is an important and complementary step in the investigation of genetic polymorphism of rhizobia and may help to elucidate rhizobial evolutionary dynamics, including adaptation to diverse environments.

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7

Fterich, A., M. Mahdhi, A. Lafuente, E. Pajuelo, M. A. Caviedes, I. D. Rodriguez-Llorente, and M. Mars. "Taxonomic and symbiotic diversity of bacteria isolated from nodules ofAcacia tortilissubsp.raddianain arid soils of Tunisia." Canadian Journal of Microbiology 58, no. 6 (June 2012): 738–51. http://dx.doi.org/10.1139/w2012-048.

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Анотація:

A collection of rhizobia isolated from Acacia tortilis subsp. raddiana nodules from various arid soils in Tunisia was analyzed for their diversity at both taxonomic and symbiotic levels. The isolates were found to be phenotypically diverse. The majority of the isolates tolerated 3% NaCl and grew at 40 °C. Genetic characterization emphasized that most of the strains (42/50) belong to the genus Ensifer , particularly the species Ensifer meliloti , Ensifer garamanticus , and Ensifer numidicus . Symbiotic properties of isolates showed diversity in their capacity to nodulate their host plant and to fix atmospheric nitrogen. The most effective isolates were closely related to E. garamanticus. Nodulation tests showed that 3 strains belonging to Mesorhizobium genus failed to renodulate their host plant, which is surprising for symbiotic rhizobia. Furthermore, our results support the presence of non-nodulating endophytic bacteria belonging to the Acinetobacter genus in legume nodules.

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8

Ramírez-Bahena, Martha-Helena, Margarita Vargas, María Martín, Carmen Tejedor, Encarna Velázquez, and Álvaro Peix. "Alfalfa microsymbionts from different ITS and nodC lineages of Ensifer meliloti and Ensifer medicae symbiovar meliloti establish efficient symbiosis with alfalfa in Spanish acid soils." Applied Microbiology and Biotechnology 99, no. 11 (January 15, 2015): 4855–65. http://dx.doi.org/10.1007/s00253-014-6347-6.

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9

Pourbabaee, Ahmad Ali, Maryam Khazaei, Hossein Ali Alikhani, and Somayeh Emami. "Root nodulation of alfalfa by Ensifer meliloti in petroleum contaminated soil." Rhizosphere 17 (March 2021): 100305. http://dx.doi.org/10.1016/j.rhisph.2021.100305.

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10

Botou, Maria, Vassilis Yalelis, Panayiota Lazou, Iliana Zantza, Konstantinos Papakostas, Vassiliki Charalambous, Emmanuel Mikros, Emmanouil Flemetakis, and Stathis Frillingos. "Specificity profile of NAT/NCS2 purine transporters in Sinorhizobium ( Ensifer ) meliloti." Molecular Microbiology 114, no. 1 (April 8, 2020): 151–71. http://dx.doi.org/10.1111/mmi.14503.

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11

Sánchez-Romero, Juan José, and Luis F. Olguin. "Choline sulfatase from Ensifer (Sinorhizobium) meliloti: Characterization of the unmodified enzyme." Biochemistry and Biophysics Reports 3 (September 2015): 161–68. http://dx.doi.org/10.1016/j.bbrep.2015.08.002.

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12

Guerrouj, Kamal, Eduardo Pérez-Valera, Hanaa Abdelmoumen, Eulogio J. Bedmar, and Mustapha Missbah El Idrissi. "Ensifer melilotiis the preferred symbiont ofMedicago arboreain eastern Morocco soils." Canadian Journal of Microbiology 59, no. 8 (August 2013): 540–48. http://dx.doi.org/10.1139/cjm-2013-0268.

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Анотація:

Polyphasic characterization of 61 bacteria isolated from root nodules of Medicago arborea (Medic tree) plants growing in 4 arid soils of the arid eastern area of Morocco was studied. All the isolates characterized were fast growers. The phenotypic, symbiotic, and cultural characteristics analyzed allowed the description of a broad physiological diversity among the isolates. The results obtained suggest that the phenotype of these rhizobia might have evolved to adapt to the local conditions. The genetic characterization consisted of an analysis of the rep-PCR (repetitive extragenic palindromic polymerase chain reaction) fingerprints and a PCR-based RFLP (restriction fragment length polymorphism) of the 16S rDNA patterns. The diversity of the isolates was investigated by rep-PCR, giving a similarity of 62%, delineated into 3 clusters, 4 groups, and 6 subclusters. This wide diversity was also observed by a phenotypic approach, where the carbohydrate assimilation test was the most discriminating. The results show a relationship between rep-PCR fingerprinting and sugar assimilation, which are complementary in diversity investigation. The nearly complete 16S rRNA gene sequence from representative strains of each soil showed they are closely related to members of the genus Ensifer of the family Rhizobiaceae within the Alphaproteobacteria and shows the highest similitude values (99.93%/100%) with Ensifer meliloti LMG 6133T(X67222). Sequencing of the symbiotic nodC gene from 7 representative strains revealed they had 94.89% identity with the nodC sequence of the type strain E. meliloti LMG 6133T(EF428922). Therefore, the 61 M. arborea isolates from the 4 different soils have the same phylogenetic affiliation, which proves the restricted host specificity among M. arborea species.

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13

Reeve, Wayne, Ross Ballard, Elizabeth Drew, Rui Tian, Lambert Bräu, Lynne Goodwin, Marcel Huntemann, et al. "Genome sequence of the Medicago-nodulating Ensifer meliloti commercial inoculant strain RRI128." Standards in Genomic Sciences 9, no. 3 (January 25, 2014): 602–13. http://dx.doi.org/10.4056/sigs.4929626.

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14

Spini, G., F. Decorosi, M. Cerboneschi, S. Tegli, A. Mengoni, C. Viti, and L. Giovannetti. "Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses." Plant and Soil 399, no. 1-2 (September 30, 2015): 159–78. http://dx.doi.org/10.1007/s11104-015-2659-2.

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15

Soares Filho, Cecilio Viega, Leonardo Aurélio Silva, Jaqueline Silva Boregio, Mariangela Hungria, Adônis Moreira, and Marco Antônio Nogueira. "Biomass Yield, Nitrogen Content and Uptake, And Nutritive Value of Alfalfa Co-Inoculated with Plant-Growth Promoting Bacteria." International Journal for Innovation Education and Research 8, no. 5 (May 1, 2020): 400–420. http://dx.doi.org/10.31686/ijier.vol8.iss5.2355.

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Alfalfa (Medicago sativa L.) has high forage yield potential, protein quality, palatability, and digestibility, and low seasonality. The aim of this study was to evaluate the effects of strains of Sinorhizobium meliloti and Azospirillum brasilense on the nutritive content, bromatological composition (crude protein [CP], neutral detergent fiber [NDF], acid detergent fiber [ADF], and in vitro digestibility of dry weight [IVDDW]), and shoot dry weight (SDW), relative chlorophyll index (RCI), number of tillers (NT), plant height (PH), and root dry weight (RDW) and volume (RV), of alfalfa grown in a Typic Ultisol. The experiment consisted of eight combinations of plant-growth promoting bacteria (PGPB). The treatments were as follows: T1:non-inoculated control without N-fertilizer (NI); T2: NI + N-fertilizer, and inoculated with T3: Sinorhizobium (=Ensifer) meliloti SEMIA 116 + N-fertilizer; T4: S. meliloti SEMIA 116 + A. brasilense Ab-V5 + Ab-V6 + N-fertilizer; T5: S. meliloti SEMIA 134 + N-fertilizer; T6: S. meliloti SEMIA 134 + co-inoculation + N-fertilizer; T7: S. meliloti SEMIA 135 + N-fertilizer; and T8: S. meliloti SEMIA 135 + co-inoculation + N-fertilizer. S. meliloti strains are used in commercial inoculants for the alfalfa, and A. brasilense for several non-legumes and legumes in Brazil. The experiment was performed for three successive cuts under greenhouse conditions. Application of N-fertilization increases the production cost, making alfalfa cultivation unviable. Inoculation with three strains of Sinorhizobium meliloti highly promoted alfalfa growth, considering several parameters, including PH, RCI, NT, SDW and RDW, nutritive value, and with an emphasis on RV, and total N content and total N accumulated in shoots and roots. No further increases were observed with the co-inoculation with the PGPB A. brasilense. Studies in field and greenhouse conditions are necessary to verify the benefits of the use of PGPB in the cultivation of alfalfa.

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16

Batstone, Rebecca T., Anna M. O’Brien, Tia L. Harrison, and Megan E. Frederickson. "Experimental evolution makes microbes more cooperative with their local host genotype." Science 370, no. 6515 (October 22, 2020): 476–78. http://dx.doi.org/10.1126/science.abb7222.

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Advances in microbiome science require a better understanding of how beneficial microbes adapt to hosts. We tested whether hosts select for more-cooperative microbial strains with a year-long evolution experiment and a cross-inoculation experiment designed to explore how nitrogen-fixing bacteria (rhizobia) adapt to legumes. We paired the bacterium Ensifer meliloti with one of five Medicago truncatula genotypes that vary in how strongly they “choose” bacterial symbionts. Independent of host choice, E. meliloti rapidly adapted to its local host genotype, and derived microbes were more beneficial when they shared evolutionary history with their host. This local adaptation was mostly limited to the symbiosis plasmids, with mutations in putative signaling genes. Thus, cooperation depends on the match between partner genotypes and increases as bacteria adapt to their local host.

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17

Belechheb, Taoufik, Omar Bouhnik, Mohammed Bakkali, Mounir Hassani Zerrouk, Amin Laglaoui, Mustapha Missbah El Idrissi, and Abdelhay Arakrak. "Ensifer. meliloti sv. lancerottense nodulates Lotus creticus in alkaline soils of Northern Morocco." Rhizosphere 18 (June 2021): 100339. http://dx.doi.org/10.1016/j.rhisph.2021.100339.

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18

Mnasri, Bacem, Yazid Badri, Sabrine Saïdi, Philippe de Lajudie, and Ridha Mhamdi. "Symbiotic diversity of Ensifer meliloti strains recovered from various legume species in Tunisia." Systematic and Applied Microbiology 32, no. 8 (December 2009): 583–92. http://dx.doi.org/10.1016/j.syapm.2009.07.007.

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19

Kang, Wenjuan, Lin Xu, Zhehao Jiang, and Shangli Shi. "Genetic diversity and symbiotic efficiency difference of endophytic rhizobia of Medicago sativa." Canadian Journal of Microbiology 65, no. 1 (January 2019): 68–83. http://dx.doi.org/10.1139/cjm-2018-0158.

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Анотація:

Research on rhizobium diversity has paved the way for diversification of rhizobial germplasm resources. Seventy-three endophytic bacterial isolates were collected from seven tissues of five alfalfa cultivars in three geographic locations in Gansu, China. Restriction fragment length polymorphism (RFLP) fingerprinting of 16S rRNA and analysis of concatenated sequence of three housekeeping genes (atpD, glnII, and recA) and two symbiotic genes (nodC and nifH) were used for strain identification. Results showed that the endophytic strains were genetically diverse at different taxonomic levels, and Ensifer meliloti (31) and Agrobacterium radiobacter (12) are common Medicago sativa endophytic bacteria in Gansu, China. The nifH genes (97%–98% sequence identity) of E. meliloti strains were more diverse than the nodC genes (99%–100% sequence identity), even though the strains evolved from a common ancestor. The degree of dispersion of symbiotic phenotypes of E. meliloti strains on M. sativa ‘Gannong No. 3’, ‘Gannong No. 9’, and ‘Qingshui’ was much less than that on M. sativa ‘Longzhong’ and ‘WL168HQ’. This suggested that the symbiotic efficiency of E. meliloti strains on the former three alfalfa cultivars was similar but on the latter two was discrepant. Their symbiotic efficiency differed primarily according to alfalfa cultivars and, to a lesser extent, to the tested strains, indicating the difference in the sensitivity of different alfalfa cultivars to rhizobial strains.

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20

Geddes, Barney A., Jason V. S. Kearsley, Jiarui Huang, Maryam Zamani, Zahed Muhammed, Leah Sather, Aakanx K. Panchal, George C. diCenzo, and Turlough M. Finan. "Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago." Proceedings of the National Academy of Sciences 118, no. 2 (December 31, 2020): e2018015118. http://dx.doi.org/10.1073/pnas.2018015118.

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Reduction of N2 gas to ammonia in legume root nodules is a key component of sustainable agricultural systems. Root nodules are the result of a symbiosis between leguminous plants and bacteria called rhizobia. Both symbiotic partners play active roles in establishing successful symbiosis and nitrogen fixation: while root nodule development is mostly controlled by the plant, the rhizobia induce nodule formation, invade, and perform N2 fixation once inside the plant cells. Many bacterial genes involved in the rhizobia–legume symbiosis are known, and there is much interest in engineering the symbiosis to include major nonlegume crops such as corn, wheat, and rice. We sought to identify and combine a minimal bacterial gene complement necessary and sufficient for symbiosis. We analyzed a model rhizobium, Sinorhizobium (Ensifer) meliloti, using a background strain in which the 1.35-Mb symbiotic megaplasmid pSymA was removed. Three regions representing 162 kb of pSymA were sufficient to recover a complete N2-fixing symbiosis with alfalfa, and a targeted assembly of this gene complement achieved high levels of symbiotic N2 fixation. The resulting gene set contained just 58 of 1,290 pSymA protein-coding genes. To generate a platform for future synthetic manipulation, the minimal symbiotic genes were reorganized into three discrete nod, nif, and fix modules. These constructs will facilitate directed studies toward expanding the symbiosis to other plant partners. They also enable forward-type approaches to identifying genetic components that may not be essential for symbiosis, but which modulate the rhizobium’s competitiveness for nodulation and the effectiveness of particular rhizobia–plant symbioses.

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21

He, Yu Rong, Jing Yu Wang, En Tao Wang, Gu Feng, Yue Li Chang, Xin Hua Sui, and Wen Xin Chen. "Trigonella arcuata-associated rhizobia—an Ensifer (Sinorhizobium) meliloti population adapted to a desert environment." Plant and Soil 345, no. 1-2 (April 5, 2011): 89–102. http://dx.doi.org/10.1007/s11104-011-0762-6.

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22

Paulucci, Natalia S., Marta S. Dardanelli та Mirta García de Lema. "Biochemical and molecular evidence of a Δ9 fatty acid desaturase from Ensifer meliloti 1021". Microbiological Research 169, № 5-6 (травень 2014): 463–68. http://dx.doi.org/10.1016/j.micres.2013.08.003.

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23

Galardini, Marco, Marco Bazzicalupo, Emanuele Biondi, Eveline Brambilla, Matteo Brilli, David Bruce, Patrick Chain, et al. "Permanent draft genome sequences of the symbiotic nitrogen fixing Ensifer meliloti strains BO21CC and AK58." Standards in Genomic Sciences 9, no. 2 (December 15, 2013): 352–33. http://dx.doi.org/10.4056/sigs.3797438.

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24

Martínez, Rosario, Ana Espejo, Manuel Sierra, Irene Ortiz-Bernad, David Correa, Eulogio Bedmar, María López-Jurado, and Jesús María Porres. "Co-inoculation of Halomonas maura and Ensifer meliloti to improve alfalfa yield in saline soils." Applied Soil Ecology 87 (March 2015): 81–86. http://dx.doi.org/10.1016/j.apsoil.2014.11.013.

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25

Ge, Feng, Ling-Yan Zhou, Ying Wang, Yuan Ma, Shan Zhai, Zhong-Hua Liu, Yi-Jun Dai, and Sheng Yuan. "Hydrolysis of the neonicotinoid insecticide thiacloprid by the N2-fixing bacterium Ensifer meliloti CGMCC 7333." International Biodeterioration & Biodegradation 93 (September 2014): 10–17. http://dx.doi.org/10.1016/j.ibiod.2014.05.001.

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26

Povolo, Silvana, and Sergio Casella. "Effect of poly-3-hydroxybutyrate synthase mutation on the metabolism of Ensifer (formerly Sinorhizobium ) meliloti." Journal of Basic Microbiology 49, no. 2 (November 24, 2008): 178–86. http://dx.doi.org/10.1002/jobm.200800139.

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27

Primo, Emiliano D., Sacha Cossovich, Fiorela Nievas, Pablo Bogino, Ethan A. Humm, Ann M. Hirsch, and Walter Giordano. "Exopolysaccharide production in Ensifer meliloti laboratory and native strains and their effects on alfalfa inoculation." Archives of Microbiology 202, no. 2 (November 3, 2019): 391–98. http://dx.doi.org/10.1007/s00203-019-01756-3.

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28

Wehner, Gwendolin, Doris Kopahnke, Klaus Richter, Steffen Kecke, Adam Schikora, and Frank Ordon. "Priming Is a Suitable Strategy to Enhance Resistance Towards Leaf Rust in Barley." Phytobiomes Journal 3, no. 1 (January 2019): 46–51. http://dx.doi.org/10.1094/pbiomes-09-18-0041-r.

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Анотація:

Priming allows plants to respond faster and stronger to abiotic or biotic stresses. Leaf rust (Puccinia hordei) is an important pathogen of barley (Hordeum vulgare), for which resistance genes are known, but mostly overcome. Therefore, the aims of this study were (i) to establish a priming system in barley, based on bacterial N-acyl homoserine lactone (AHL), and (ii) to get information on the effect of priming on the reaction to leaf rust. Plants were inoculated with bacteria, i.e., Ensifer meliloti with repaired expR copy, producing the oxo-C14-homoserine lactone (AHL) and an E. meliloti strain carrying the attM lactonase gene from Agrobacterium tumefaciens, which cleaves the AHL and acts here as negative control. After three bacterial inoculations, plants were challenged with P. hordei strain I-80 at the three leaves stage. Twelve days after infection, scoring of the leaf area diseased and the infection type was conducted followed by the calculation of the relative susceptibility. First results indicate a significantly (P < 0.001) higher resistance level to P. hordei after inoculation with E. meliloti. Furthermore, significant (P < 0.001) differences were detected between the accessions tested for priming efficiency, which can be the basis to screen a larger set of barley accessions to detect quantitative trait loci or candidate genes involved in priming. [Formula: see text] Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

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29

De Benedetto, Juan P., Ricardo García, and Alejandro Perticari. "Efecto de la inoculación con diferentes cepas Ensifer meliloti sobre el rendimiento de forraje de Alfalfa." Agrotecnia, no. 25 (November 30, 2017): 46. http://dx.doi.org/10.30972/agr.0252483.

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Roumiantseva, Marina L., Maria E. Vladimirova, Alla S. Saksaganskaia, Victoria S. Muntyan, Alexandra P. Kozlova, Alexey M. Afonin, Olga A. Baturina, and Boris V. Simarov. "Ensifer meliloti L6-AK89, an Effective Inoculant of Medicago lupulina Varieties: Phenotypic and Deep-Genome Screening." Agronomy 12, no. 4 (March 22, 2022): 766. http://dx.doi.org/10.3390/agronomy12040766.

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This paper presents a deep analysis of the accessory genome of an economically promising strain of Ensifer (Sinorhizobium) meliloti, L6-AK89, obtained as a result of next-generation high-throughput sequencing (MiSeq, MinIon). Strain L6-AK89 is a StrR mutant of the native strain CIAM1775, a symbiont of Medicago lupulina that adapted to a saline and arid habitat in NW Kazakhstan. CIAM1775 is an effective inoculant of M. lupulina cv. Mira (fodder type standard), cultivated on moderately acid soils in the NW agricultural region of Russia. Strain L6-AK89 makes it possible to obtain the expected high (>150%) increases in dry mass of the same plant variety in plant tests. The L6-AK89 genome has an increased proportion of sequences related to the accessory elements relative to reference strain Rm1021, 7.4% versus 4.8%. A set of 53 nod/noe/nol/nif/fdx/fix genes and 32 genes involved in stress tolerance together with 16S rRNA and recA–atpD–glnII–gyrB–dnaJ were evaluated. The high symbiotic efficiency of L6-АК89 with hop clover is most likely due to unique features of its genome, in combination with structural differences in its nod and stress-related genes, as well as unique clusters of quorum-sensing genes and osmoprotector synthesis.

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Gubry-Rangin, Cécile, Gilles Béna, Jean-Claude Cleyet-Marel, and Brigitte Brunel. "Definition and evolution of a new symbiovar, sv. rigiduloides, among Ensifer meliloti efficiently nodulating Medicago species." Systematic and Applied Microbiology 36, no. 7 (October 2013): 490–96. http://dx.doi.org/10.1016/j.syapm.2013.06.004.

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32

Bromfield, E. S. P., J. T. Tambong, S. Cloutier, D. Prévost, G. Laguerre, P. van Berkum, T. V. Tran Thi, R. Assabgui, and L. R. Barran. "Ensifer, Phyllobacterium and Rhizobium species occupy nodules of Medicago sativa (alfalfa) and Melilotus alba (sweet clover) grown at a Canadian site without a history of cultivation." Microbiology 156, no. 2 (February 1, 2010): 505–20. http://dx.doi.org/10.1099/mic.0.034058-0.

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Phage-resistant and -susceptible bacteria from nodules of alfalfa and sweet clover, grown at a site without a known history of cultivation, were identified as diverse genotypes of Ensifer, Rhizobium and Phyllobacterium species based on sequence analysis of ribosomal (16S and 23S rRNA) and protein-encoding (atpD and recA) genes, Southern hybridization/RFLP and a range of phenotypic characteristics. Among phage-resistant bacteria, one genotype of Rhizobium sp. predominated on alfalfa (frequency ∼68 %) but was recovered infrequently (∼1 %) from sweet clover. A second genotype was isolated infrequently only from alfalfa. These genotypes fixed nitrogen poorly in association with sweet clover and Phaseolus vulgaris, but were moderately effective with alfalfa. They produced a near-neutral reaction on mineral salts agar containing mannitol, which is atypical of the genus Rhizobium. A single isolate of Ensifer sp. and two of Phyllobacterium sp. were recovered only from sweet clover. All were highly resistant to multiple antibiotics. Phylogenetic analysis indicated that Ensifer sp. strain T173 is closely related to, but separate from, the non-symbiotic species ‘Sinorhizobium morelense’. Strain T173 is unique in that it possesses a 175 kb symbiotic plasmid and elicits ineffective nodules on alfalfa, sweet clover, Medicago lupulina and Macroptilium atropurpureum. The two Phyllobacterium spp. were non-symbiotic and probably represent bacterial opportunists. Three genotypes of E. meliloti that were symbiotically effective with alfalfa and sweet clover were encountered infrequently. Among phage-susceptible isolates, two genotypes of E. medicae were encountered infrequently and were highly effective with alfalfa, sweet clover and Medicago polymorpha. The ecological and practical implications of the findings are discussed.

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Wehner, Gwendolin, Adam Schikora, Frank Ordon, and Torsten Will. "Priming negatively affects feeding behaviour and aphid biomass of Rhopalosiphum padi on barley." Journal of Pest Science 94, no. 4 (February 9, 2021): 1237–47. http://dx.doi.org/10.1007/s10340-021-01329-8.

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AbstractPlants have developed numerous strategies for responding to abiotic and biotic stresses. In particular, the microbiota surrounding plants may have a positive effect on plant stress responses. One is the reaction to rhizobacteria, which can lead to induced systemic resistance. Gram-negative soil bacteria that produce N-acyl homoserine lactones (AHL), for example, Ensifer meliloti, induce a primed state in plants that is part of the inducible resistance phenomenon. Observing Rhopalosiphum padi feeding behaviour on a priming sensitive barley genotype, treated with the AHL-producing E. meliloti strain expR + ch, using electrical penetration graph technique showed decreased ingestion of food. Aphids appear to overcome this effect within the eight-hour observation period, possibly explaining the absence of differences of reproduction. Reproduction was observed for a period of 14 days on primed and control-treated plants. Long-term observations over a period of 40 days after aphid infestation showed a lower aphid biomass in contrast to a control group, interpreted as delayed population growth, and an increase in the biomass of barley plants. Priming-related genotypic effects of the defence response to aphids were observed, with no beneficial effects on the plant genotype when its sensitivity to priming was low. Previously, an AHL-priming sensitive barley genotype showed enhanced resistance against fungi when primed with the expR + ch strain of E. meliloti. The present study reports the same effect against R. padi. These findings suggest that sensitivity to AHL-priming may represent a new approach for plant breeding, targeting multiple pests in parallel by induced plant resistance.

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Buntic, Aneta, Olivera Stajkovic-Srbinovic, Magdalena Knezevic, Djordje Kuzmanovic, Natasa Rasulic, and Dusica Delic. "Development of liquid rhizobial inoculants and pre-inoculation of alfalfa seeds." Archives of Biological Sciences 71, no. 2 (2019): 379–87. http://dx.doi.org/10.2298/abs181008062b.

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Application of liquid microbial inoculants on legume seeds is a sustainable agricultural practice that can improve plant nutrient uptake and increase crop productivity. Inoculants should provide long-term survival of rhizobia in the final product and after application, to legume seeds. Ten different medium formulations of microbial inoculants were examined (yeast mannitol broth with the addition of agar, sodium-alginate, calcium chloride, glycerol or ferric chloride and combinations thereof) for the survival of the efficient nitrogen-fixing rhizobium, Sinorhizobium (Ensifer) meliloti L3Si strain. The most suitable liquid inoculant for survival of L3Si during a storage time of 150 days was the medium formulation containing glycerol in combination with agar or sodium-alginate. Alfalfa seeds were pre-inoculated with four formulations (yeast mannitol broth (YMB), YMB with agar (1 g L-1), YMB with 1 or 5 g L-1 sodium-alginate) for up to three months. Seeds pre-inoculated and stored for one month produced successful alfalfa plants. The nitrogen content in alfalfa obtained from pre-inoculated seeds one month before sowing was adequate and ranged from 3.72-4.19%. Using S. meliloti-based liquid inoculants for alfalfa and application of the pre-inoculation technique can increase the quality of alfalfa crops and reduce cultivation cost.

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Jia, Rui Zong, En Tao Wang, Jing Hui Liu, Ying Li, Jun Gu, Hong Li Yuan, and Wen Xin Chen. "Effectiveness of different Ensifer meliloti strain-alfalfa cultivar combinations and their influence on nodulation of native rhizobia." Soil Biology and Biochemistry 57 (February 2013): 960–63. http://dx.doi.org/10.1016/j.soilbio.2012.07.021.

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Abd-El-Karem, Yasser, Tanja Elbers, Rudolf Reichelt, and Alexander Steinbüchel. "Heterologous expression of Anabaena sp. PCC7120 cyanophycin metabolism genes cphA1 and cphB1 in Sinorhizobium (Ensifer) meliloti 1021." Applied Microbiology and Biotechnology 89, no. 4 (October 12, 2010): 1177–92. http://dx.doi.org/10.1007/s00253-010-2891-x.

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37

Defez, Valenti, Andreozzi, Romano, Ciaramella, Pesaresi, Forlani, and Bianco. "New Insights into Structural and Functional Roles of Indole-3-acetic acid (IAA): Changes in DNA Topology and Gene Expression in Bacteria." Biomolecules 9, no. 10 (September 23, 2019): 522. http://dx.doi.org/10.3390/biom9100522.

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: Indole-3-acetic acid (IAA) is a major plant hormone that affects many cellular processes in plants, bacteria, yeast, and human cells through still unknown mechanisms. In this study, we demonstrated that the IAA-treatment of two unrelated bacteria, the Ensifer meliloti 1021 and Escherichia coli, harboring two different host range plasmids, influences the supercoiled state of the two plasmid DNAs in vivo. Results obtained from in vitro assays show that IAA interacts with DNA, leading to DNA conformational changes commonly induced by intercalating agents. We provide evidence that IAA inhibits the activity of the type IA topoisomerase, which regulates the DNA topological state in bacteria, through the relaxation of the negative supercoiled DNA. In addition, we demonstrate that the treatment of E. meliloti cells with IAA induces the expression of some genes, including the ones related to nitrogen fixation. In contrast, these genes were significantly repressed by the treatment with novobiocin, which reduces the DNA supercoiling in bacterial cells. Taking into account the overall results reported, we hypothesize that the IAA action and the DNA structure/function might be correlated and involved in the regulation of gene expression. This work points out that checking whether IAA influences the DNA topology under physiological conditions could be a useful strategy to clarify the mechanism of action of this hormone, not only in plants but also in other unrelated organisms.

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Terpolilli, Jason, Yvette Hill, Rui Tian, John Howieson, Lambert Bräu, Lynne Goodwin, James Han, et al. "Genome sequence of Ensifer meliloti strain WSM1022; a highly effective microsymbiont of the model legume Medicago truncatula A17." Standards in Genomic Sciences 9, no. 2 (December 15, 2013): 315–24. http://dx.doi.org/10.4056/sigs.4608286.

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39

Amarelle, Vanesa, Federico Rosconi, Juan Manuel Lázaro-Martínez, Graciela Buldain, Francisco Noya, Mark R. O’Brian, and Elena Fabiano. "HmuS and HmuQ of Ensifer/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo." BioMetals 29, no. 2 (February 23, 2016): 333–47. http://dx.doi.org/10.1007/s10534-016-9919-3.

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40

Robledo, Marta, Esther Menéndez, Jose Ignacio Jiménez-Zurdo, Raúl Rivas, Encarna Velázquez, Eustoquio Martínez-Molina, Giles Oldroyd, and Pedro F. Mateos. "Heterologous Expression of Rhizobial CelC2 Cellulase Impairs Symbiotic Signaling and Nodulation in Medicago truncatula." Molecular Plant-Microbe Interactions® 31, no. 5 (May 2018): 568–75. http://dx.doi.org/10.1094/mpmi-11-17-0265-r.

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The infection of legume plants by rhizobia is tightly regulated to ensure accurate bacterial penetration, infection, and development of functionally efficient nitrogen-fixing root nodules. Rhizobial Nod factors (NF) have key roles in the elicitation of nodulation signaling. Infection of white clover roots also involves the tightly regulated specific breakdown of the noncrystalline apex of cell walls in growing root hairs, which is mediated by Rhizobium leguminosarum bv. trifolii cellulase CelC2. Here, we have analyzed the impact of this endoglucanase on symbiotic signaling in the model legume Medicago truncatula. Ensifer meliloti constitutively expressing celC gene exhibited delayed nodulation and elicited aberrant ineffective nodules, hampering plant growth in the absence of nitrogen. Cotreatment of roots with NF and CelC2 altered Ca2+ spiking in root hairs and induction of the early nodulin gene ENOD11. Our data suggest that CelC2 alters early signaling between partners in the rhizobia-legume interaction.

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Kim, Minsoo, Yuhui Chen, Jiejun Xi, Christopher Waters, Rujin Chen, and Dong Wang. "An antimicrobial peptide essential for bacterial survival in the nitrogen-fixing symbiosis." Proceedings of the National Academy of Sciences 112, no. 49 (November 23, 2015): 15238–43. http://dx.doi.org/10.1073/pnas.1500123112.

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In the nitrogen-fixing symbiosis between legume hosts and rhizobia, the bacteria are engulfed by a plant cell membrane to become intracellular organelles. In the model legume Medicago truncatula, internalization and differentiation of Sinorhizobium (also known as Ensifer) meliloti is a prerequisite for nitrogen fixation. The host mechanisms that ensure the long-term survival of differentiating intracellular bacteria (bacteroids) in this unusual association are unclear. The M. truncatula defective nitrogen fixation4 (dnf4) mutant is unable to form a productive symbiosis, even though late symbiotic marker genes are expressed in mutant nodules. We discovered that in the dnf4 mutant, bacteroids can apparently differentiate, but they fail to persist within host cells in the process. We found that the DNF4 gene encodes NCR211, a member of the family of nodule-specific cysteine-rich (NCR) peptides. The phenotype of dnf4 suggests that NCR211 acts to promote the intracellular survival of differentiating bacteroids. The greatest expression of DNF4 was observed in the nodule interzone II-III, where bacteroids undergo differentiation. A translational fusion of DNF4 with GFP localizes to the peribacteroid space, and synthetic NCR211 prevents free-living S. meliloti from forming colonies, in contrast to mock controls, suggesting that DNF4 may interact with bacteroids directly or indirectly for its function. Our findings indicate that a successful symbiosis requires host effectors that not only induce bacterial differentiation, but also that maintain intracellular bacteroids during the host–symbiont interaction. The discovery of NCR211 peptides that maintain bacterial survival inside host cells has important implications for improving legume crops.

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Redondo, Francisco J., Teodoro Coba de la Peña, M. Mercedes Lucas, and José J. Pueyo. "Alfalfa nodules elicited by a flavodoxin-overexpressing Ensifer meliloti strain display nitrogen-fixing activity with enhanced tolerance to salinity stress." Planta 236, no. 6 (August 4, 2012): 1687–700. http://dx.doi.org/10.1007/s00425-012-1725-8.

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Toniutti, María Antonieta, and Laura Viviana Fornasero. "Efecto de la inoculación con rizobios y la fertilización fosfatada sobre la nodulación y producción de alfalfa (Medicago sativa L.) en el centro de Santa Fe (Argentina)." AgriScientia 37, no. 2 (December 30, 2020): 1–10. http://dx.doi.org/10.31047/1668.298x.v37.n2.24067.

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La simbiosis entre rizobios y alfalfa (Medicago sativa L.) contribuye a la incorporación de nitrógeno en los agroecosistemas a través de la fijación biológica, que dependerá de la adecuada provisión de nutrientes disponibles en el suelo. El objetivo de este trabajo fue evaluar el efecto de la inoculación con Ensifer meliloti y la fertilización con 0, 20 y 40 kg/ha de fósforo sobre la nodulación y productividad de una pastura de alfalfa en el centro de la provincia de Santa Fe. La inoculación y la aplicación de dosis crecientes de fósforo aumentaron el número de nódulos, biomasa nodular y la producción del forraje de las plantas. La técnica de la inoculación condujo a un incremento de la producción de materia seca entre 23 y 58 % para los tratamientos con el máximo nivel de fertilizante a los 120, 180 y 240 días de la siembra. La adición de la cepa de rizobio utilizada como inoculante y la fertilización fosfatada constituyeron una estrategia favorable para incrementar la producción de alfalfa.

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Hansen, Thomas, Bettina Schlichting, Martina Felgendreher, and Peter Schönheit. "Cupin-Type Phosphoglucose Isomerases (Cupin-PGIs) Constitute a Novel Metal-Dependent PGI Family Representing a Convergent Line of PGI Evolution." Journal of Bacteriology 187, no. 5 (March 1, 2005): 1621–31. http://dx.doi.org/10.1128/jb.187.5.1621-1631.2005.

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ABSTRACT Cupin-type phosphoglucose isomerases (cPGIs) were identified in some archaeal and bacterial genomes and the respective coding function of cpgi's from the euryarchaeota Archaeoglobus fulgidus and Methanosarcina mazei, as well as the bacteria Salmonella enterica serovar Typhimurium and Ensifer meliloti, was proven by functional overexpression. These cPGIs and the cPGIs from Pyrococcus and Thermococcus spp. represent the cPGI family and were compared with respect to kinetic, inhibitory, thermophilic, and metal-binding properties. cPGIs showed a high specificity for the substrates fructose-6-phosphate and glucose-6-phosphate and were inhibited by millimolar concentrations of sorbitol-6-phosphate, erythrose-4-phosphate, and 6-phosphogluconate. Treatment of cPGIs with EDTA resulted in a complete loss of catalytic activity, which could be regained by the addition of some divalent cations, most effectively by Fe2+ and Ni2+, indicating a metal dependence of cPGI activity. The motifs TX3PX3GXEX3TXGHXHX6-11EXY and PPX3HX3N were deduced as the two signature patterns of the novel cPGI family. Phylogenetic analysis suggests lateral gene transfer for the bacterial cPGIs from euryarchaeota.

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45

Torres, M. J., A. Hidalgo-García, E. J. Bedmar, and M. J. Delgado. "Functional analysis of the copy 1 of the fixNOQP operon of Ensifer meliloti under free-living micro-oxic and symbiotic conditions." Journal of Applied Microbiology 114, no. 6 (March 15, 2013): 1772–81. http://dx.doi.org/10.1111/jam.12168.

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46

Zhao, Yun-Xiu, Lei-Lei Guo, Shi-Lei Sun, Jing-jing Guo, and Yi-Jun Dai. "Bioconversion of indole-3-acetonitrile by the N2-fixing bacterium Ensifer meliloti CGMCC 7333 and its Escherichia coli-expressed nitrile hydratase." International Microbiology 23, no. 2 (August 13, 2019): 225–32. http://dx.doi.org/10.1007/s10123-019-00094-0.

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47

León-Barrios, Milagros, María José Lorite, Javier Donate-Correa, and Juan Sanjuán. "Ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with Lotus endemic to the Canary Islands and shows distinctive symbiotic genotypes and host range." Systematic and Applied Microbiology 32, no. 6 (September 2009): 413–20. http://dx.doi.org/10.1016/j.syapm.2009.04.003.

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48

Paço, Ana, José Rodrigo da-Silva, Denise Pereira Torres, Bernard R. Glick, and Clarisse Brígido. "Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration." Plants 9, no. 12 (November 24, 2020): 1630. http://dx.doi.org/10.3390/plants9121630.

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Manganese (Mn) toxicity is a very common soil stress around the world, which is responsible for low soil fertility. This manuscript evaluates the effect of the endophytic bacterium Pseudomonas sp. Q1 on different rhizobial-legume symbioses in the absence and presence of Mn toxicity. Three legume species, Cicer arietinum (chickpea), Trifolium subterraneum (subterranean clover), and Medicago polymorpha (burr medic) were used. To evaluate the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase produced by strain Q1 in these interactions, an ACC deaminase knockout mutant of this strain was constructed and used in those trials. The Q1 strain only promoted the symbiotic performance of Rhizobium leguminosarum bv. trifolii ATCC 14480T and Ensifer meliloti ATCC 9930T, leading to an increase of the growth of their hosts in both conditions. Notably, the acdS gene disruption of strain Q1 abolished the beneficial effect of this bacterium as well as causing this mutant strain to act deleteriously in those specific symbioses. This study suggests that the addition of non-rhizobia with functional ACC deaminase may be a strategy to improve the pasture legume–rhizobial symbioses, particularly when the use of rhizobial strains alone does not yield the expected results due to their difficulty in competing with native strains or in adapting to inhibitory soil conditions.

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Shrestha, Abhishek, Ahmed Elhady, Shimaa Adss, Gwendolin Wehner, Christoph Böttcher, Holger Heuer, Frank Ordon, and Adam Schikora. "Genetic Differences in Barley Govern the Responsiveness to N-Acyl Homoserine Lactone." Phytobiomes Journal 3, no. 3 (January 2019): 191–202. http://dx.doi.org/10.1094/pbiomes-03-19-0015-r.

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Enhanced resistance in barley (Hordeum vulgare) against pathogens, such as the powdery mildew-causing fungus Blumeria graminis f. sp. hordei, is of high importance. The beneficial effects of bacterial quorum sensing molecules on resistance and plant growth have been shown in different plant species. Here, we present the effects of the N-3-oxotetradecanoyl-l-homoserine lactone (oxo-C14-HSL) on the resistance of different barley genotypes. Genetically diverse accessions of barley were identified and exposed to the beneficial, oxo-C14-HSL-producing bacterium Ensifer meliloti or the pure N-acyl homoserine lactone (AHL) molecule. Metabolic profiling along with expression analysis of selected genes and physiological assays revealed that the capacity to react varies among different barley genotypes. We demonstrate that upon pretreatment with AHL molecule, AHL-primable barley genotype expresses enhanced resistance against B. graminis f. sp. hordei. We further show that pretreatment with AHL correlates with stronger activation of barley MAP kinases and regulation of defense-related PR1 and PR17b genes after a subsequent treatment with chitin. Noticeable was the stronger accumulation of lignin. Our results suggest that appropriate genetic background is required for AHL-induced priming. At the same time, they bear potential to use these genetic features for new breeding and plant protection approaches.

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García-Fraile, Paula, Raúl Rivas, Anne Willems, Alvaro Peix, Miet Martens, Eustoquio Martínez-Molina, Pedro F. Mateos, and Encarna Velázquez. "Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba." International Journal of Systematic and Evolutionary Microbiology 57, no. 4 (April 1, 2007): 844–48. http://dx.doi.org/10.1099/ijs.0.64680-0.

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During a study of polysaccharide-hydrolysing bacteria present in different plant sources, two strains were isolated from pulverized decaying wood of Populus alba and classified in the genus Rhizobium on basis of their almost complete 16S rRNA gene sequences. Their closest phylogenetic relatives were Rhizobium galegae USDA 4128T and Rhizobium huautlense S02T, with 98.2 and 98.1 % 16S rRNA gene sequence similarity, respectively. recA and atpD sequence analysis showed that these species have less than 88 and 92 % similarity, respectively, to the novel strains. In contrast to their closest phylogenetic relatives, the two strains showed strong cellulase activity on plates containing CM-cellulose as a carbon source. They were also distinguishable from these species on the basis of other phenotypic characteristics. The strains were able to induce ineffective nodules on Medicago sativa and the sequence of their nodD gene was phylogenetically close to that of Ensifer meliloti 1021 (99.6 % similarity). DNA–DNA hybridization values ranged from 10 to 22 % with respect to R. galegae USDA 4128T and 14 to 25 % with respect to R. huautlense S02T, showing that the strains from this study belong to a novel species, for which the name Rhizobium cellulosilyticum sp. nov. is proposed. The type strain is ALA10B2T (=LMG 23642T=DSM 18291T=CECT 7176T).

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