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Добірка наукової літератури з теми "Ensifer meliloti"

Автор: Grafiati

Опубліковано: 10 березня 2023

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Статті в журналах з теми "Ensifer meliloti"

1

Torres, Maria J., Maria I. Rubia, Teodoro de la Peña, José J. Pueyo, Eulogio J. Bedmar, and María J. Delgado. "Genetic basis for denitrification in Ensifer meliloti." BMC Microbiology 14, no. 1 (2014): 142. http://dx.doi.org/10.1186/1471-2180-14-142.

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2

Baxter, Laura, Proyash Roy, Emma Picot, Jess Watts, Alex Jones, Helen Wilkinson, Patrick Schäfer, Miriam Gifford, and Beatriz Lagunas. "Comparative Genomics across Three Ensifer Species Using a New Complete Genome Sequence of the Medicago Symbiont Sinorhizobium (Ensifer) meliloti WSM1022." Microorganisms 9, no. 12 (November 25, 2021): 2428. http://dx.doi.org/10.3390/microorganisms9122428.

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Here, we report an improved and complete genome sequence of Sinorhizobium (Ensifer) meliloti strain WSM1022, a microsymbiont of Medicago species, revealing its tripartite structure. This improved genome sequence was generated combining Illumina and Oxford nanopore sequencing technologies to better understand the symbiotic properties of the bacterium. The 6.75 Mb WSM1022 genome consists of three scaffolds, corresponding to a chromosome (3.70 Mb) and the pSymA (1.38 Mb) and pSymB (1.66 Mb) megaplasmids. The assembly has an average GC content of 62.2% and a mean coverage of 77X. Genome annotation of WSM1022 predicted 6058 protein coding sequences (CDSs), 202 pseudogenes, 9 rRNAs (3 each of 5S, 16S, and 23S), 55 tRNAs, and 4 ncRNAs. We compared the genome of WSM1022 to two other rhizobial strains, closely related Sinorhizobium (Ensifer) meliloti Sm1021 and Sinorhizobium (Ensifer) medicae WSM419. Both WSM1022 and WSM419 species are high-efficiency rhizobial strains when in symbiosis with Medicago truncatula, whereas Sm1021 is ineffective. Our findings report significant genomic differences across the three strains with some similarities between the meliloti strains and some others between the high efficiency strains WSM1022 and WSM419. The addition of this high-quality rhizobial genome sequence in conjunction with comparative analyses will help to unravel the features that make a rhizobial symbiont highly efficient for nitrogen fixation.

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3

Muratova, Anna, Natalya Pozdnyakova, Oleg Makarov, Mikhail Baboshin, Boris Baskunov, Nina Myasoedova, Ludmila Golovleva, and Olga Turkovskaya. "Degradation of phenanthrene by the rhizobacterium Ensifer meliloti." Biodegradation 25, no. 6 (July 23, 2014): 787–95. http://dx.doi.org/10.1007/s10532-014-9699-9.

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4

Sun, Shi-Lei, Tian-Qi Lu, Wen-Long Yang, Jing-Jing Guo, Xue Rui, Shi-Yun Mao, Ling-Yan Zhou, and Yi-Jun Dai. "Characterization of a versatile nitrile hydratase of the neonicotinoid thiacloprid-degrading bacterium Ensifer meliloti CGMCC 7333." RSC Advances 6, no. 19 (2016): 15501–8. http://dx.doi.org/10.1039/c5ra27966f.

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Анотація:

The nitrogen-fixing bacterium Ensifer meliloti CGMCC 7333 and its nitrile hydratase (NHase) degrade the neonicotinoid insecticides, thiacloprid (THI) and acetamiprid (ACE), to their corresponding amide metabolites.

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5

MAREK-KOZACZUK, MONIKA, JERZY WIELBO, ANNA PAWLIK, and ANNA SKORUPSKA. "Nodulation competitiveness of Ensifer meliloti alfalfa Nodule Isolates and Their Potential for Application as Inoculants." Polish Journal of Microbiology 63, no. 4 (2014): 357–86. http://dx.doi.org/10.33073/pjm-2014-051.

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Alfalfa (Medicago sativa) is a widely cultivated legume, which enters into nitrogen-fixing symbiosis with Ensifer (Sinorhizobium) spp. In this study, an autochthonous rhizobial population of Ensifer sp. occupying alfalfa nodules grown in arable soil was used as the basis for selection of potential inoculants. Alfalfa nodule isolates were identified as Ensifer meliloti by partial 16S rDNA, recA, atpD and nodC nucleotide sequencing. The sampled isolates displayed different symbiotic performance and diversity in the number of plasmids and molecular weight. Isolates that were the most efficient in symbiotic nitrogen fixation were tagged with a constitutively expressed gusA gene carried by a stable plasmid vector pJBA21Tc and used in competition experiments in soil under greenhouse conditions. Two E. meliloti strains LU09 and LU12, which effectively competed with indigenous soil rhizobia, were selected. The metabolic profiles of these selected strains showed differences in the use of carbon and energy sources. In addition, the LU09 strain exhibited bacteriocin production and LU12 mineral phosphate solubilization, which are valuable traits for soil survival. These strains may be considered as potential biofertilizers for alfalfa cultivation.

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6

Biondi, Emanuele G., Enrico Tatti, Diego Comparini, Elisa Giuntini, Stefano Mocali, Luciana Giovannetti, Marco Bazzicalupo, Alessio Mengoni, and Carlo Viti. "Metabolic Capacity of Sinorhizobium (Ensifer) meliloti Strains as Determined by Phenotype MicroArray Analysis." Applied and Environmental Microbiology 75, no. 16 (June 26, 2009): 5396–404. http://dx.doi.org/10.1128/aem.00196-09.

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ABSTRACT Sinorhizobium meliloti is a soil bacterium that fixes atmospheric nitrogen in plant roots. The high genetic diversity of its natural populations has been the subject of extensive analysis. Recent genomic studies of several isolates revealed a high content of variable genes, suggesting a correspondingly large phenotypic differentiation among strains of S. meliloti. Here, using the Phenotype MicroArray (PM) system, hundreds of different growth conditions were tested in order to compare the metabolic capabilities of the laboratory reference strain Rm1021 with those of four natural S. meliloti isolates previously analyzed by comparative genomic hybridization (CGH). The results of PM analysis showed that most phenotypic differences involved carbon source utilization and tolerance to osmolytes and pH, while fewer differences were scored for nitrogen, phosphorus, and sulfur source utilization. Only the variability of the tested strain in tolerance to sodium nitrite and ammonium sulfate of pH 8 was hypothesized to be associated with the genetic polymorphisms detected by CGH analysis. Colony and cell morphologies and the ability to nodulate Medicago truncatula plants were also compared, revealing further phenotypic diversity. Overall, our results suggest that the study of functional (phenotypic) variability of S. meliloti populations is an important and complementary step in the investigation of genetic polymorphism of rhizobia and may help to elucidate rhizobial evolutionary dynamics, including adaptation to diverse environments.

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7

Fterich, A., M. Mahdhi, A. Lafuente, E. Pajuelo, M. A. Caviedes, I. D. Rodriguez-Llorente, and M. Mars. "Taxonomic and symbiotic diversity of bacteria isolated from nodules ofAcacia tortilissubsp.raddianain arid soils of Tunisia." Canadian Journal of Microbiology 58, no. 6 (June 2012): 738–51. http://dx.doi.org/10.1139/w2012-048.

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A collection of rhizobia isolated from Acacia tortilis subsp. raddiana nodules from various arid soils in Tunisia was analyzed for their diversity at both taxonomic and symbiotic levels. The isolates were found to be phenotypically diverse. The majority of the isolates tolerated 3% NaCl and grew at 40 °C. Genetic characterization emphasized that most of the strains (42/50) belong to the genus Ensifer , particularly the species Ensifer meliloti , Ensifer garamanticus , and Ensifer numidicus . Symbiotic properties of isolates showed diversity in their capacity to nodulate their host plant and to fix atmospheric nitrogen. The most effective isolates were closely related to E. garamanticus. Nodulation tests showed that 3 strains belonging to Mesorhizobium genus failed to renodulate their host plant, which is surprising for symbiotic rhizobia. Furthermore, our results support the presence of non-nodulating endophytic bacteria belonging to the Acinetobacter genus in legume nodules.

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8

Ramírez-Bahena, Martha-Helena, Margarita Vargas, María Martín, Carmen Tejedor, Encarna Velázquez, and Álvaro Peix. "Alfalfa microsymbionts from different ITS and nodC lineages of Ensifer meliloti and Ensifer medicae symbiovar meliloti establish efficient symbiosis with alfalfa in Spanish acid soils." Applied Microbiology and Biotechnology 99, no. 11 (January 15, 2015): 4855–65. http://dx.doi.org/10.1007/s00253-014-6347-6.

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9

Pourbabaee, Ahmad Ali, Maryam Khazaei, Hossein Ali Alikhani, and Somayeh Emami. "Root nodulation of alfalfa by Ensifer meliloti in petroleum contaminated soil." Rhizosphere 17 (March 2021): 100305. http://dx.doi.org/10.1016/j.rhisph.2021.100305.

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10

Botou, Maria, Vassilis Yalelis, Panayiota Lazou, Iliana Zantza, Konstantinos Papakostas, Vassiliki Charalambous, Emmanuel Mikros, Emmanouil Flemetakis, and Stathis Frillingos. "Specificity profile of NAT/NCS2 purine transporters in Sinorhizobium ( Ensifer ) meliloti." Molecular Microbiology 114, no. 1 (April 8, 2020): 151–71. http://dx.doi.org/10.1111/mmi.14503.

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Більше джерел

Дисертації з теми "Ensifer meliloti"

1

Neumann, Ute [Verfasser]. "Ko-Inokulation von Rhizobien (Ensifer meliloti) und Azospirillum : In vitro Effekte auf Luzerne (und ko-kultivierte Birken) und die mikrobiellen Gemeinschaften ihrer Rhizosphären in unterschiedlichen Böden / Ute Neumann." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2011. http://d-nb.info/1018206531/34.

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2

Selmi, Hela. "Effet de l'ajout de biochar sur la symbiose tripartite Ensifer meliloti-Rhizophagus irregularis-luzerne (Medicago sativa L.), sur la production d'inocula bactériens et envers la lutte aux agents pathogènes." Master's thesis, Université Laval, 2016. http://hdl.handle.net/20.500.11794/26598.

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L’agriculture durable repose sur l’utilisation de moins d’intrants chimiques et favorise l’utilisation des produits biologiques comme le biochar. Ce dernier, à certaines doses, peut stimuler la fixation biologique d’azote chez les légumineuses. Notre projet vise à étudier l’effet d’un biochar de copeaux de pin pyrolysés à 700 ºC sur la symbiose tripartite Ensifer meliloti-Rhizophagus irregularis-Medicago sativa L., sur la production d’inocula bactériens et envers la lutte contre les agents pathogènes. Une culture de luzerne inoculée avec deux E. meliloti A2 et S14, en présence du Rhizophagus irregularis et dans un sol amendé avec 0, 15 ou 30 % de biochar (v:v) a été réalisée. Une stimulation significative de la mycorhization a été observée en présence de 15 % de biochar, et des souches A2 et S14. Par contre, l’inoculation de la luzerne avec A2 ou S14 n’a pas eu d’effet significatif sur les rendements, indiquant la présence dans le sol de souches efficaces d’E. meliloti. Une étude portant sur la survie des rhizobiums et du Bacillus subtilis MBI 600 menée pendant 120 jours à 4 et 25 °C. Elle a montré que 15 % de biochar favorise significativement la survie des cellules de rhizobiums. Par contre, pour Bacillus subtilis c’est le Pro-mix à 100 % qui supporte mieux la survie des cellules. Ainsi, l’effet de biochar sur la survie des microorganismes dépend de la nature de ces derniers et de la dose utilisée. L’étude de l’effet des trois doses du biochar sur les agents pathogènes a montré qu’à forte dose il pourrait favoriser le développement des pathogènes. Les résultats apportent des pistes pour les modalités d’application de biochar en agriculture. Par contre, notre étude est réalisée avec un seul type de biochar à trois doses. Il sera donc très important de tester d’autres types ainsi que d’autres doses de biochar.
Sustainable agriculture is based on the use of less chemical inputs and promotes the use of biological products such as biochar. Many studies clearly indicate that some biochars can stimulate biological nitrogen fixation in legumes. Our project aims to study the effect of a biochar (Pines, 700ºC) on the tripartite symbiosis Ensifer meliloti-Rhizophagus irregularis-Medicago sativa L., on the production of bacterial inocula and on the fight against pathogens. A culture of alfalfa inoculated with two strains of E. meliloti A2 or S14, in the presence of Rhizophagus irregularis and in a soil amended with 0, 15 or 30% of biochar (vol:vol) was conducted. A significant stimulation of mycorrhization was observed in the presence of 15% biochar and A2 or S14. However, inoculation of alfalfa with A2 or S14 had no significant effect on yields, indicating the presence in soil of effective strains of E. meliloti. A study on survival of rhizobia and Bacillus subtilis MBI 600 at 4°C and 25°C was conducted for 120 days. It showed that biochar (15%, vol:vol) promotes significantly the survival of rhizobial cells but for Bacillus subtilis it is the Pro-mix without biochar which supports better cell survival. Thus, the biochar effect on survival of microorganisms depends on the dose of biochar used. The effect of biochar amendments on P. ultimum and FORL colonization and infection was also evaluated. The study of the effect of three doses of biochar (0, 15, 30%; vol:vol) on pathogens showed that high doses may offer a good environment for pathogens development. Our study was performed using a single type of biochar with three doses. Therefore, it is very important to test other types and doses of biochar to be able to make recommendations.

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3

SPINI, GIULIA. "Response of the symbiont Ensifer meliloti to the plant flavonoid lutelin: not only symbiosis." Doctoral thesis, 2016. http://hdl.handle.net/2158/1028231.

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The present work was mainly addressed to provide an extensive phenotypic investigation of the luteolin effects on the phenotypes of E. meliloti to get an interpretative framework in modeling luteolin-induced metabolic switches. In the context of the luteolin-responsive phenotypes, the changes dependent or independent from the NodD regulation (i.e the major luteolin sensor) were elucidated using a deletion nodD mutant of E. meliloti and the possible contribution of other luteolin mediators, beyond NodD, was then investigated.

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Частини книг з теми "Ensifer meliloti"

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Missbah El Idrissi, Mustapha, Mohammed Ourarhi, Omar Bouhnik, and Hanaa Abdelmoumen. "Ensifer meliloti is the main microsymbiont of Prosopis chilensis in arid soils of Eastern Morocco." In Microbial Symbionts, 111–31. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-99334-0.00033-5.

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