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1

Mignolli, Francesco, Graciela Beatriz Rojas, and María Laura Vidoz. "Supraoptimal ethylene acts antagonistically with exogenous gibberellins during Solanum lycopersicum (Solanaceae) hypocotyl growth." Boletín de la Sociedad Argentina de Botánica 51, no. 2 (June 15, 2016): 235–42. http://dx.doi.org/10.31055/1851.2372.v51.n2.14836.

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In many plant species, ethylene and gibberellins interact to regulate plant growth and development. In some cases, these hormones can act in a synergistic way whereas in others they can be antagonistic. To date, the control of hypocotyl elongation by ethylene and gibberellins has been poorly explored in tomato. In this paper, we report that, application of exogenous ethylene to tomato seedlings or high endogenous ethylene production, as in the epinastic mutant, strongly prevent the effect of gibberellic acid (GA3) application. Moreover, constitutive activation of gibberellin signal in a DELLA deficient mutant is not able to counteract the inhibitory effect of ethylene on hypocotyl elongation, suggesting that ethylene acts independently from DELLA-mediated gibberellin response. Interestingly, when ethylene perception is blocked, the GA3 promotive effect on hypocotyl length is less effective, indicating that the presence of a basal level of ethylene could synergistically enhance hypocotyl growth. Taken together, these observations may suggest that, in tomato, supraoptimal concentrations of ethylene are able to antagonize gibberellin effect but normal levels seem to promote gibberellin-induced hypocotyl elongation.
2

Janas, Krystyna M., and Jiři Šebánek. "Effect of cotyledons and epicotyl upon the activity of endogenous gibberellins in roots of flax (Linum usitatissimum L.) seedlings." Acta Agrobotanica 34, no. 2 (2013): 231–33. http://dx.doi.org/10.5586/aa.1981.017.

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In 11-day-old flax seedlings, the level of endogenous gibberellins in roots decreased within 12 hours after the excision of cotyledons and the epicotyl; however, 24 to 48 hours after excision the gibberellin level increased again. The decrease in the gibberellin level within the first 12 hours after excision suggests a participation of the cotyledons and the epicotyl in the biosynthesis of endogenous gibberellins.
3

Sabovljevic, Aneta, Marko Sabovljevic, and D. Grubisic. "Gibberellin influence on the morphogenesis of the moss Bryum argenteum Hedw. in in vitro conditions." Archives of Biological Sciences 62, no. 2 (2010): 373–80. http://dx.doi.org/10.2298/abs1002373s.

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The moss Bryum argenteum Hedw. was treated with gibberellins as well as some inhibitors of gibberellin biosynthesis in order to investigate their influence on B. argenteum morphogenesis. Generally, gibberellins have not been chemically identified in bryophytes, while other groups of classical phytohormones (auxins, cytokinins, abscisic acid and ethylene) have been chemically identified in these plants. The in vitro culture of the moss Bryum argenteum was established from sterilized spores. The apical shoots of untreated gametophytes grown in vitro were used to investigate the influence of different substances on secondary protonema and on the growth and multiplication of the gametophytes. B. argenteum reacts differently to the growth regulators applied. Both gibberellins applied in vitro (GA3 and GA7) have a positive effect on B. argenteum morphogenesis. Shoot multiplication was negatively affected by three tested growth retardants (ancymidol, BX-112 and chlorocholine chloride), while these substances did not have such strong effects on the moss protonema development.
4

Verma, Meena, Roop Singh Bora, Imran Sheikh, Vinod Kumar, Punesh Sangwan, and Harcharan Singh Dhaliwal. "Effect of gibberellins and ascorbic acid treatment on phytic acid and micronutrients dialyzability in germinated biofortified wheat seeds." Indian Journal of Community Health 33, no. 1 (March 31, 2021): 123–29. http://dx.doi.org/10.47203/ijch.2021.v33i01.017.

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Introduction: Phytic acid chelate minerals, including Fe and Zn and render them inaccessible once ingested by human beings. The evaluation of differences in the dialyzability of macronutrients including Fe and Zn in various wheat derivatives is therefore important for the enhancement of nutritional quality of grains. Objectives: The objective of current study was to improve the micronutrient content in wheat grain. Methods: During germination (12, 24, 48, 72, 96 h), effects of gibberellins and ascorbic acid on phytic acid content as well as dialyzability of iron and zinc of wheat derivatives were determined. Results: The phytic acid content in wheat flour was determined and it was found 7.61 to 7.48 mg/g. After the treatment with gibberellins, it was significantly reduced from 8.68 to 21.6 % and 9.65 to 20.9 % with ascorbic acid. In wheat flour dialyzabilty of Fe was 4.53 to 8.97 mg/kg. After germination, Fe content was increased from 9.77 to 32.0 % with gibberellin, and 13.9 to 31.0 % with ascorbic acid. Moreover, with gibberellins, Zn content was increased from 8.68 to 21.6 % and 9.65 to 20.9 % with ascorbic acid, respectively. Conclusion: These results suggested that gibberellins as well as ascorbic acid can be exploited to improve the dialyzability of iron and zinc content due to reduced antinutrient i. e phytic acid and make the minerals available for the absorption in monogastric animals including human beings.
5

Castro-Camba, Ricardo, Conchi Sánchez, Nieves Vidal, and Jesús Mª Vielba. "Plant Development and Crop Yield: The Role of Gibberellins." Plants 11, no. 19 (October 9, 2022): 2650. http://dx.doi.org/10.3390/plants11192650.

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Gibberellins have been classically related to a few key developmental processes, thus being essential for the accurate unfolding of plant genetic programs. After more than a century of research, over one hundred different gibberellins have been described. There is a continuously increasing interest in gibberellins research because of their relevant role in the so-called "Green Revolution", as well as their current and possible applications in crop improvement. The functions attributed to gibberellins have been traditionally restricted to the regulation of plant stature, seed germination, and flowering. Nonetheless, research in the last years has shown that these functions extend to many other relevant processes. In this review, the current knowledge on gibberellins homeostasis and mode of action is briefly outlined, while specific attention is focused on the many different responses in which gibberellins take part. Thus, those genes and proteins identified as being involved in the regulation of gibberellin responses in model and non-model species are highlighted. The present review aims to provide a comprehensive picture of the state-of-the-art perception of gibberellins molecular biology and its effects on plant development. This picture might be helpful to enhance our current understanding of gibberellins biology and provide the know-how for the development of more accurate research and breeding programs.
6

Wiseman, Nadine J., and Colin G. N. Turnbull. "Effects of photoperiod and paclobutrazol on growth dynamics of petioles in strawberry (Fragaria × ananassa)." Functional Plant Biology 26, no. 4 (1999): 353. http://dx.doi.org/10.1071/pp98001.

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Petiole elongation of strawberry plants (Fragaria × ananassa) is modified by photoperiod, and gibberellins may mediate this response. To test the relationship further, we measured petiole growth dynamics under short and long days, in comparison with responses to paclobutrazol, which reduces gibberellin biosynthesis. Growth rate in short days was lower after 2 days, and at its maximum was 60% of long-day controls. Petiole length at maturity was 75%, and was accounted for primarily by decreased cell length. Subsequent leaves showed similar patterns, but in the third leaf, cell number was also reduced. Petiole growth rate after treatment with paclobutrazol (5 mg plant–1) was maximal at 2 days, 44% of the control maximum at day 7. Mature length was 30% of controls, again due to reduced cell length. Second leaves had even more severely reduced growth rates. Increasing paclobutrazol concentration over the range 0.025–1.0 mg progressively reduced growth, but a gibberellin-independent component accounted for 20% of elongation. Effects of combined paclobutrazol and photoperiod treatments were additive. The normal short-day response therefore occurred in paclobutrazol-treated plants with already slowed growth and reduced capacity for gibberellin synthesis. We conclude that assessment of the function of gibberellins requires direct measurement of gibberellins in petioles during growth.
7

Moncur, M. W., G. F. Rasmussen, and O. Hasan. "Effect of paclobutrazol on flower-bud production in Eucalyptusnitens espalier seed orchards." Canadian Journal of Forest Research 24, no. 1 (January 1, 1994): 46–49. http://dx.doi.org/10.1139/x94-007.

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Eucalyptusnitens (Dean & Maiden) Maiden grafts growing as espaliers at Ridgley, Tasmania, and Canberra, Australian Capital Territory, were treated with paclobutrazol as a single collar drench application. Umbel production was enhanced for 3 years at Ridgley and 2 years at Canberra. Canberra grafts were also treated by trunk injection, which proved effective only in the 1st year. Untreated grafts did not produce an appreciable number of umbels until year 3. Paclobutrazol reduced the level of endogenous gibberellins. Close association between gibberellin levels and increases in reproductive activity of E. nitens grafts suggests that the effects of paclobutrazol treatment may be mediated via a reduction in the rate of gibberellin biosynthesis.
8

Adelina, Enny, Nuraeni Nuraeni, Yohanis Tambing, and Ida Musdalifa. "EFFECTIVENESS OF GIBBERELLINS IN BREAKING DORMANCY OF SNAKEFRUIT SEEDS (SALACCA SNAKEFRUIT GEARTNER)." AGROLAND The Agricultural Sciences Journal (e-Journal) 7, no. 2 (December 23, 2020): 91–98. http://dx.doi.org/10.22487/agroland.v7i2.616.

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This study aims to examine the effectiveness of gibberellins in breaking dormancy to produce high viability snakefruit (Salacca snakefruit Geartner) seeds. The materials used are snakefruit seeds from Tamareja Village, Donggala Regency, sterile sand media, aquades, and GA3 solution. This research was arranged using a completely randomized design (CRD) with one factor, namely the provision of gibberellin, consisting of five levels of concentration, namely P0 = control (without treatment), P1 = 30 ppm and P2 = 40 ppm P3 = 50 ppm P4 = 60 ppm. each treatment was repeated five times so that there were 25 experimental units. Each experimental unit used 20 seeds. Observation data were analyzed using analysis of variance (ANOVA), followed by Tukey' HSD test if the treatment tested showed a significant effect. The results showed that gibberellin administration effectively broke snakefruit seeds' dormancy and produced seeds with high viability. The higher the gibberellins' concentration would increase germination speed plumule length, radicle length, and faster embryo axis emergence. Gibberellin 60 ppm resulted in germination power 98%, germination rate 20.97 days, plumule length 5.52 cm, radicle length 5.15 cm, and embryo axis emergence 9.2 days.
9

Basnizki, Jehuda, and Eliezer E. Goldschmidt. "FURTHER EXAMINATION OF GIBBERELLIN A, EFFECTS ON FLOWERING OF GLOBE ARTICHOKES (CYNARA SCOLYMUS L.) UNDER CONTROLLED ENVIRONMENT AND FIELD CONDITIONS." Israel Journal of Plant Sciences 42, no. 2 (May 13, 1994): 159–66. http://dx.doi.org/10.1080/07929978.1994.10676567.

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The effect of gibberellin A3 (GA3) treatments on flowering of seed-grown globe artichoke (Cynara scolymus) was investigated under controlled environment and field conditions. GA3 induced flower initiation in lines ‘060’ and ‘Talpiot’ under strictly noninductive, short-day-high-temperature conditions. In ‘Talpiot’, GA3 induced microscopically detectable flower initiation but no stem elongation, suggesting involvement of gibberellins in the flower formation process. Under field conditions, GA3 replaced the cold requirements of line ‘HU 271’, thereby enabling the start of flowering during autumn. The vegetative clone Bianca d'Espana flowered during autumn without GA3 treatment, probably due to its minimum cold requirements. Definition of the response type and the role of gibberellins in the flowering of globe artichoke are discussed.
10

Murrinie, Endang Dewi, Untung Sudjianto, and Khoirinnidha Ma’rufa Ma’rufa. "PENGARUH GIBERELIN TERHADAP PERKECAMBAHAN BENIH DAN PERTUMBUHAN SEMAI KAWISTA (Feronia Limonia (L.) Swingle)." Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto 23, no. 2 (December 28, 2021): 183. http://dx.doi.org/10.30595/agritech.v23i2.12614.

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Wood-apple is a rare plant that is almost extinct, so efforts are needed to increase the population. Wood-apple is generally propagated generatively. Wood-apple seeds need 2-3 weeks to germinate, so when germinated many seeds are rotten before germination. Soaking in gibberellins is one way that can be done to shorten germination. The study aimed to determine the effect of concentration and duration of soaking in gibberellins on germination and growth of wood-apple seedlings. The study consisted of two factors which were designed in a completely randomized design. The first factor was the concentration of gibberellins, consisting of three levels, namely 25 ppm (k1), 50 ppm (k2), and 75 ppm (k3). The second factor was soaking time, consisting of three levels, namely 6 hours (l1), 9 hours (12), and 12 hours (13), so there were 9 treatment combinations and one treatment without gibberellins (control), each treatment was repeated three times. The results showed that the gibberellin treatment increased the height of the seedlings compared to the control. The concentration of gibberellins affects the length and diameter of the hypocotyl and the length of the radicle. Concentrations of 50 and 75 ppm resulted in higher hypocotyl diameter and radicle length than 25 ppm. The concentration of 75 ppm gave the highest hypocotyl length. Soaking time affects the percentage of germination, germination rate, and growth rate of wood-apple seedings. Soaking in gibberellins for 12 hours gave a faster growth rate of seedlings than 6 and 9 hours.
11

Puspitasari, Diah Rochana, Anne Nuraini, and Sumadi Sumadi. "Pematahan Dormansi Umbi Bawang Putih (Allium sativum L.) Varietas Lumbu Hijau dengan Perlakuan Lama Penyimpanan Umbi pada Suhu Rendah dan Aplikasi Giberelin." Paspalum: Jurnal Ilmiah Pertanian 8, no. 2 (September 30, 2020): 85. http://dx.doi.org/10.35138/paspalum.v8i2.189.

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Low temperature and gibberellin treatments affected on breaking dormancy. The effects of low temperature in storage duration and gibberellin treatrments on breaking of dormancy of garlic were examined in this study. The aims of this study to determinate the best treatments for breaking garlic bulb dormancy. The sorted garlic bulbs were stored at 4 ± 0.4 ° C for 0 (control), 15, 30 and 45 days. The bulbs that have been treated by low temperature then treated by gibberellins at various concentrations including 0, 75, 150, and 225 mgL-1 by soaking for 24 hours. The bulbs were planted in the seedling house. The low temperature storage (4±0,4ᵒC) for 45 days is more effective than 0, 15 and 30 days, while the concentration of gibberellins were not have a significant effect on breaking dormancy. GA3 endogenous was increased during low temperature storage, on the other hand, it accelerated on breaking dormancy . The highest percentage of sprouting bulb produced by treatment at low temperature storage for 45 days. Growth parameters such as shoot length, shoot dry weight and leaves get the best at 45 days storage time. Low temperature storage (4±0,4ᵒC) is effective in breaking dormancy of garlic bulb.
12

da Silva, Gustavo Cabral, Ivan de-la-Cruz-Chacón, Ana Beatriz Marques Honório, Bruna Cavinatti Martin, Marília Caixeta Sousa, Felipe Girotto Campos, Carmen Sílvia Fernandes Boaro, and Gisela Ferreira. "Temperature and GA3 as Modulating Factors in the Biosynthesis of Alkaloids during Imbibition and Early Development of Annona x atemoya Mabb. cv. ‘Gefner’ Seedlings." Horticulturae 8, no. 9 (August 26, 2022): 766. http://dx.doi.org/10.3390/horticulturae8090766.

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Alkaloids are products of the specialized metabolism of plants and temperature is a factor capable of modulating their biosynthesis. Species of the Annonaceae family biosynthesize alkaloids and present dormancy in their seeds, which can be overcome with the use of gibberellins. Therefore, the aim of this work was to evaluate whether temperature variations and the use of gibberellin in seeds affect the production of alkaloids during germination and early development of Annona x atemoya Mabb. cv. ‘Gefner’ seedlings. Results showed that the temperature of 30 °C associated with imbibition in water caused an increase in the production of total alkaloids and liriodenine and that the use of gibberellin decreased production. In addition, it was possible to identify the presence of nine other alkaloids with organ-specific distribution. The presence of none of them was induced by the effect of temperature or gibberellic acid. Therefore, it could be concluded that temperature variation and the use of GA3 alter the biosynthesis of alkaloids, with high temperature causing increased concentration, but the use of GA3 reducing production.
13

Farida, Farida, and Nani Rohaeni. "PENGARUH KONSENTRASI HORMON GIBERELIN TERHADAP PERTUMBUHAN DAN HASIL TANAMAN OKRA." ZIRAA'AH MAJALAH ILMIAH PERTANIAN 44, no. 1 (February 12, 2019): 1. http://dx.doi.org/10.31602/zmip.v44i1.1601.

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The effect of gibberellins hormone concentration on the growth and yield of okra. The aims of research are (1) to determinate the effects of growth and yield of okra plant with gibberellins hormon treatment, (2) to determine the best concentration of gibberellns hormone on the growth and yield of okra plants. This research obtained for three mounth on January to Aprl 2018. The research was conducted in Diponegoro Street, Village Sangatta Sub-distrct East Kutai. This research uses is Completely Randomized Design (CRD) of non factorial experiments each of four treatments and six replcates, are : g0 = without fertilizer, g1 = 150 ppm, g2 = 200 ppm, g3 = 250 ppm. The obtained data was analyzed by F test 1% and 5% and be continued with LSD at 5% if the treatment had significant effect. The results of the best growth research plant height 7 MST (75,141 cm) and growth of the leaves number 7 MST, best flowering age at 32,167 day and the best yield production result is 971,333 gram solid treatment.
14

Michalski, Leszek. "Effects of ionizing radiation on changes in the level of gibberellin-like substances during Scotch pine seeds germination." Acta Societatis Botanicorum Poloniae 45, no. 3 (2015): 263–69. http://dx.doi.org/10.5586/asbp.1976.022.

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The level of gibberellin-like substances in pine seeds exposed to ionizing radiation was investigated using extraction, fractionation, acid hydrolysis, partitioning on chromatographic columns and biological tests. It was found that the level of free and bound gibberellin-like substances is dependent on the applied gamma radiation dose. It is assumed that ionizing radiation may re-lease bound gibberellins into free ones.
15

Wang, Bing, and Alan R. Langille. "Response of a Gibberellin-deficient Potato Mutant to Induction and Growth Regulators as a Working Model for Tuber Initiation." HortScience 42, no. 3 (June 2007): 540–43. http://dx.doi.org/10.21273/hortsci.42.3.540.

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To explore further the role endogenous gibberellins play in the formation of the potato tuber, a gibberellin-deficient dwarf of Solanum tuberosum subsp. andigena (PI 281036) and its normal sibling were used in this study. Because gibberellins are known to be elevated and to affect tuber formation negatively when potato plants are grown under long day conditions, test plants were first grown in a growth chamber under conditions that did not favor tuberization (noninducing conditions). The stem apices of dwarfs received weekly applications of a 100-ppm gibberellic acid (GA3) solution to achieve growth similar to that of normal plants. When the requisite height was achieved, five dwarfs and five normal sibs were treated to foliar runoff with a 100-ppm GA3 solution, and a like number of these plants received a control spray of distilled water. These plants were then placed in a growth chamber adjusted to inducing conditions for tuber initiation. An additional five dwarfs and five normal sibs were sprayed with 600 ppm paclobutrazol (PB), a gibberellin inhibitor, and a similar number of plants received a distilled water control spray. This group of plants was returned to the noninducing chamber. After 1 week, plants were removed from their respective growth chambers and divided into two-node apical, medial, and basal leaf-bud cuttings. Basal buds of the cuttings were buried in moist potting mix in a mist chamber with a 16-hour photoperiod. Rhizome and tuberization responses were evaluated after 3 weeks. The experiment was repeated and results combined for statistical treatment. Orthogonal contrasts revealed that apical cuttings from normal donor plants produced rhizomes only under noninducing conditions or when treated with GA3. No rhizomes formed on apical cuttings from normal plants growing under inducing conditions (favoring tuberization) or noninduced plants receiving PB (a gibberellin inhibitor). For apical dwarf tissues, there were no effects of treatments on rhizome production, except for the PB treatment, which resulted in shorter rhizomes. Tuberization was observed in apical tissues of induced and noninduced dwarfs, which lack the ability to synthesize gibberellin, but only in induced cuttings of normal sibs. Noninduced dwarf cuttings tuberized as well as those from normal plants receiving the antigibberellin treatment. Tuber weights from induced apical cuttings of dwarfs and normal sibs were not significantly different. These results support the significant role played by gibberellins in tuber formation.
16

Al-Shahwany, Ayyad W., Fatima K. Mohammad, and Ropak Tofiq. "Influence Soaking Gibberellin acid on seed germination for Citrus limetta and Citrus limonum and their seedlings growth." Journal of Biotechnology Research Center 8, no. 1 (January 1, 2014): 43–49. http://dx.doi.org/10.24126/jobrc.2014.8.1.301.

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Study was undertaken to test the effects of soaking seeds Citrus limetta and Citurs limonum in Gibberellic acid (GA3) solutions 0, 100, 500, 1000 mg / L for 24 hours prior on the rate of germination, some morphological characteristics for seedlings also were investigated in this study. Factorial experiment was carried out 4×2×5 in a randomized complete block design with five replicates for each type of seed during the period of March to September 2011. The results showed significant differences in most traits, as it reached the highest percentage of germination for the treatment with 500 mg/l for both types of seeds, while the highest dry and fresh weights of shoot and root, main root length, highest shoot, leaves number and leaf area index were in 1000 mg /l treatment. No significant effect of GA3 treatment on the amount of A, B and total chlorophyll in the seedlings leaves. The best treatment was soaking seed in 1000gm/l Gibberellic acid. The information documented the stimulating effects of seedling be used to increase the ratio of germination. Also gibberellins stimulated effects continues for some seedlings morphological characteristics and progressively with gibberellins seed treatments influence vary according to genotypes of citrus seeds, that can increase concentrations which used in soaking seeds solutions.
17

Han, Jennifer, Jan E. Murray, Qingyi Yu, Paul H. Moore, and Ray Ming. "The Effects of Gibberellic Acid on Sex Expression and Secondary Sexual Characteristics in Papaya." HortScience 49, no. 3 (March 2014): 378–83. http://dx.doi.org/10.21273/hortsci.49.3.378.

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The vegetative forms of male (XY), female (XX), and hermaphrodite (XYh) papaya (Carica papaya L.) plants are phenotypically identical. However, the flower and inflorescence morphology of each sex type is unique. Gynodioecious varieties SunUp, SunUp Diminutive mutant, and dioecious AU9 were used to test the response of papaya to gibberellic acid (GA3). Exogenous applications of GA3 on female and hermaphrodite flowers of papaya did not yield any sex reversal phenotype but caused a significant increase in peduncle elongation and inflorescence branch number in all treated plants. An increase in flower number was seen in females but not hermaphrodites or males. There was an increase in plant height for all treated plants except SunUp Diminutive mutant, suggesting that the mechanism causing the dwarf phenotype is independent of gibberellins. Gibberellin metabolism genes were identified in the papaya genome, none of which mapped to the sex-determining region of either the male- or hermaphrodite-specific region of papaya Y or Yh chromosome. We hypothesize that a transacting regulatory element that enhances gibberellin biosynthesis plays a role in the extreme length of the male papaya peduncle.
18

Harkess, Richard L., and Robert E. Lyons. "Gibberellin- and Cytokinin-induced Growth and Flowering Responses in Rudbeckia hirta L." HortScience 29, no. 3 (March 1994): 141–42. http://dx.doi.org/10.21273/hortsci.29.3.141.

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BA and GA4+7, were applied to vegetative, mature Rudbeckia hirta plants at the beginning of long days (LD). There were no synergistic effects, but BA inconsistently affected branching and had no effect on flowering. Floral initiation of the terminal inflorescence was promoted by GA4+7, although axillary inflorescences were not. Increasing GA4+7 levels decreased the time to terminal inflorescence anthesis. However, the interval between the terminal and second axillary inflorescence anthesis was increased. The net result was no significant effect on the time to second axillary inflorescence anthesis. Gibberellins may enhance the LD effect on the apical meristem of Rudbeckia, but axillary meristems, which initiate later, remained unaffected. Chemical names used: benzyladenine (BA), gibberellin4+7, (GA4+7).
19

Jaques, Lanes B. A., Ivan R. Carvalho, Vinícius J. Szareski, João R. Pimentel, Cristian Troyjack, Simone M. Dellagostin, Mayara T. Mendonça, et al. "Gibberellic Acid Utilization in Seeds and Plants of Beans: Effect on Growth and Seeds Physiological Quality." Journal of Agricultural Science 11, no. 2 (January 15, 2019): 541. http://dx.doi.org/10.5539/jas.v11n2p541.

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The growth regulators from gibberellins, when applied on plants, might improve physic and physiological features, stimulate cell division and elongation, this study aimed at evaluating the influence of gibberellic acid doses applied exogenously on bean crop through seed treatment, and analyzing its effects on morphological attributes of seedlings and physiological quality of the seeds produced. The experimental design was completely randomized design, with treatments corresponding to the doses: zero (distilled water only), 50, 100 and 200 mg L-1, arranged in four replicates. The use of gibberellic acid in bean seeds did not result in changes on the number of leaves, leaf area, dry mass of leaves and stem. Applications of foliar gibberellic acid potentiated stem height and root dry mass of seedlings.
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Zhang, L., S. Rajapakse, R. E. Ballard, and N. C. Rajapakse. "Light Quality Regulation of Gene Expression in Chrysanthemum." HortScience 33, no. 3 (June 1998): 446c—446. http://dx.doi.org/10.21273/hortsci.33.3.446c.

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A major part of Clemson light quality research program focuses on understanding the mechanisms of plant growth alteration under spectral filters. We have extended this research to understand the molecular basis for plant height control by spectral filters. From the whole plant research we have found that gibberellins (GA) play a key role in height control under spectral filters. However, it is not clear whether an alteration of endogenous gibberellin levels or a change in sensitivity to gibberellins is causing the observed effects. Since gibberellins are a key hormone in cell expansion and flower development, an understanding of gibberellin regulation and metabolism under spectral filters is critical for the control of height and flowering of plants grown under these filters. For the molecular study, we have selected the gene that code for enzyme GA 20-oxidase, one of the key enzymes involved in producing functional GAs in the mevalonic acid pathway. GA 20-oxidase is thought to be a site of regulation of GA synthesis by the environment. We are currently studying the regulation of the expression of this gene by light quality using Northern analysis. Results from temporal and tissue specific regulation of chrysanthemum plants grown under CuSO4 filters will be presented.
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Webber, J. E., S. D. Ross, R. P. Pharis, and J. N. Owens. "Interaction between gibberellin A4/7 and root-pruning on the reproductive and vegetative process in Douglas-fir. II. Effects on shoot elongation and its relationship to flowering." Canadian Journal of Forest Research 15, no. 2 (April 1, 1985): 348–53. http://dx.doi.org/10.1139/x85-056.

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Shoot elongation and female flowering response were assessed for gibberellin A4/7 (GA4/7) and root-pruning (RP) treatments in 9- and 10-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings of families with good- and poor-flowering histories. In families with a poor-flowering history, stem injections of GA4/7 significantly enhanced elongation of third-whorl terminal shoots but produced no flowering response. In families with a good-flowering history, GA4/7 treatment had no effect on shoot elongation but did result in a significant increase in seed-cone buds. In contrast, root-pruning significantly retarded shoot growth in families with both good- and poor-flowering histories and was also the single most effective treatment for enhancing flowering. Combined, GA4/7 plus RP had a synergistic effect on flowering and GA4/7 partially overcame the inhibition of shoot growth caused by RP alone. These results are consistent with a hypothesis that exogenous and endogenous gibberellins are used preferentially for vegetative growth processes, with increased flowering occurring only after a threshold concentration of effector gibberellins is reached.
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Swiegers, Hendrik Willem, Barbara Karpinska, Yan Hu, Ian C. Dodd, Anna-Maria Botha, and Christine H. Foyer. "The Effects of High CO2 and Strigolactones on Shoot Branching and Aphid–Plant Compatibility Control in Pea." International Journal of Molecular Sciences 23, no. 20 (October 12, 2022): 12160. http://dx.doi.org/10.3390/ijms232012160.

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Elevated atmospheric CO2 concentrations (eCO2) regulate plant architecture and susceptibility to insects. We explored the mechanisms underpinning these responses in wild type (WT) peas and mutants defective in either strigolactone (SL) synthesis or signaling. All genotypes had increased shoot height and branching, dry weights and carbohydrate levels under eCO2, demonstrating that SLs are not required for shoot acclimation to eCO2. Since shoot levels of jasmonic acid (JA) and salicylic acid (SA) tended to be lower in SL signaling mutants than the WT under ambient conditions, we compared pea aphid performance on these lines under both CO2 conditions. Aphid fecundity was increased in the SL mutants compared to the WT under both ambient and eCO2 conditions. Aphid infestation significantly decreased levels of JA, isopentenyladenine, trans-zeatin and gibberellin A4 and increased ethylene precursor ACC, gibberellin A1, gibberellic acid (GA3) and SA accumulation in all lines. However, GA3 levels were increased less in the SL signaling mutants than the WT. These studies provide new insights into phytohormone responses in this specific aphid/host interaction and suggest that SLs and gibberellins are part of the network of phytohormones that participate in host susceptibility.
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Pizarro, Alberto, and Carmen Díaz-Sala. "Effect of polar auxin transport and gibberellins on xylem formation in pine cuttings under adventitious rooting conditions." Israel Journal of Plant Sciences 67, no. 1-2 (February 25, 2020): 27–39. http://dx.doi.org/10.1163/22238980-20191120.

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Maturation-related decline of adventitious root formation is one of the major factors affecting adventitious rooting in forest tree species. We demonstrate that inhibition of polar auxin transport promoted cambium and xylem differentiation in rooting-competent hypocotyl cuttings from Pinus radiata under conditions of adventitious root formation. Treatments with bioactive gibberellins inhibited rooting while at the same time inducing both the differentiation of a continuous ring of cambium and xylem formation. Treatments with inhibitors of gibberellin biosynthesis did not affect the rooting response. The results demonstrate that xylem parenchyma and procambial cells at the xylem poles of rooting-competent hypocotyl cuttings after excision and under conditions of adventitious root induction become adventitious root meristems or xylem, depending on the directional auxin flow. Gibberellin may interact with this pathway, inducing xylem differentiation and inhibiting rooting. We conclude that modifications of auxin flow at the rooting sites, and the priming of cambial cells to differentiate into xylem during tree ageing, may be associated with the maturation-related decline of adventitious root formation.
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Gökbayrak, Zeliha, Hakan Engin, Arda Akçal, and Hatice Kiraz. "Pollen of Arbutus unedo: Effects of plant growth regulators." Botanica Serbica 44, no. 1 (2020): 55–59. http://dx.doi.org/10.2298/botserb2001055g.

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Arbutus unedo is a species mainly used for landscaping purposes and lately for honey production. Its antioxidant characteristics have also been a subject of of research interest. The germination of pollen grains freshly collected from flowers was tested in vitro, and 30% sucrose gave the highest pollen germination. Subsequently, three growth regulators belonging to the categories of gibberellins and brassinosteroids [24-epibrassinolide (Ebl) and 22S,23S-homobrassinolide (Hbl)] were added to the growth medium singly or in double combinations (gibberellin + brassinosteroid), and the petri dishes were kept for 24 hours at 26?1?C. The results showed that the highest germination rates were obtained from treatments with 0.01 ppm Hbl (45.47%) and 0.001 ppm Hbl (26.74%). They were followed by treatments with 0.001 ppm Ebl, 25 ppm GA3 and 0.1 ppm Ebl. As the concentration of GA3 increased, the germination rate declined considerably. Statistical analysis of the combined treatments showed that combinations of growth regulators lowered the germination rates compared to their individual application. Moreover, action of the lowest GA3 concentration (25 ppm) best matched the effects of brassinosteroids, and increasing the GA3 concentration in combined treatments did not improve germination. The highest germination was obtained with 0.1 ppm Hbl, followed by all Ebl treatments. Brassinosteroids might be an inducer of pollen germination in plants depending on their type and concentrations. When individually included in the growth medium, the extent of this induction was greater with the homobrassinolide than with the epibrassinolide. Their interactions with gibberellins are shown to be mostly dependent on the concentration of gibberellic acid used. The tested epibrassinolide was more consistent than the homobrassinolide in maintaining higher germination levels. The findings of this study indicate that gathering more information from studies involving other plant species is needed to clarify the role of brassinosteroids in in vitro and in vivo germination.
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Jazuli, MI, SN Aini, and NS Khodijah. "PEMANFAATAN GIBERELIN UNTUK MEMACU PERTUMBUHAN DAN PRODUKSI MELON MENGGUNAKAN HIDROPONIK SISTEM SUMBU." Jurnal Bioindustri 4, no. 1 (November 30, 2021): 1–11. http://dx.doi.org/10.31326/jbio.v4i1.1220.

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Melon production has decrease due to decreasing agriculture land area. An effort to increases melon production can be done by cultivating melons using a hydroponic system. Gibberellins can be used to increase melon production in Bangka Belitung. This research aims to determine the effect and gibberellin doses that has increase melon growth and production which cultivated using hydroponic wick system. This research was conducted from January to April 2020 at the Research and Experimental Station, Faculty of Agriculture, Fisheries and Biology, Universitas Bangka Belitung. The experimental design used in this study was a single completely randomized design (CRD) with various gibberellin concentrations (0 ppm, 20 ppm, 40 ppm, 60 ppm, 80 ppm, 100 ppm). Gibberellin was applied by giving it at the soaking seeds period and by spraying it onto the leaves of the plants. The results of study showed that the application of gibberellin (GA3) had a significant effect on stem diameter, leaf area, fruit weight, fruit diameter, pulp thickness and total plant dissolved solids. The application of gibberellin with 100 ppm concentration is the best treatment to increasing growth and 80 ppm concentration is the best treatment to increasing yield of melon with wick hydroponic cultivation system.
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Miceli, Alessandro, Alessandra Moncada, Leo Sabatino, and Filippo Vetrano. "Effect of Gibberellic Acid on Growth, Yield, and Quality of Leaf Lettuce and Rocket Grown in a Floating System." Agronomy 9, no. 7 (July 16, 2019): 382. http://dx.doi.org/10.3390/agronomy9070382.

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Gibberellins (GAs) are growth hormones strongly involved in a wide variety of physiological activities. Currently, gibberellins are commercially used to enhance phenotypic characteristics, earliness, and productivity of many vegetable and ornamental crops. In this work, the efficacy of supplementation of low levels of gibberellic acid (0, 10−8, 10−6, and 10−4 M GA3) through the mineral nutrient solution of a floating system on yield and quality of leaf lettuce and rocket plants was tested. The marketability of plants was lost when 10−4 M GA3 was added to the mineral nutrient solution. This study demonstrated that the addition of 10−4 M GA3 exceeded the acceptable threshold for use in hydroponics production systems. Below the concentration of 10−4 M, the presence of GA3 in the mineral nutrient solutions (MNS), especially at 10−6 M GA3, stimulated plant growth and enhanced the yield. Various morphological and physiological traits were enhanced by GA3 treatments (biomass accumulation, leaf expansion, stomatal conductance, water use efficiency (WUE), Nitrogen use efficiency (NUE), etc.), with superimposable trends in both lettuce and rocket. The addition of 10−6 M GA3 to the nutrient solution of a hydroponic floating system can promote growth and quality of lettuce and rocket plants.
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Vattiprolu, N. M. R., D. J. Woolley, and B. M. van Hooijdonk. "EFFECT OF GIBBERELLINS AND ANTI-GIBBERELLINS ON THE VEGETATIVE GROWTH OF 'HAYWARD' KIWIFRUIT (ACTINIDIA DELICIOSA)." Acta Horticulturae, no. 913 (November 2011): 433–39. http://dx.doi.org/10.17660/actahortic.2011.913.58.

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Tarigan, Sri Murti, Eka Bobby Febrianto, and Lufy Andria Cik. "PENGARUH KONSENTRASI GIBERELIN (GA3) DENGAN WAKTU APLIKASI SEBELUM PANEN TERHADAP MUTU FISIK TANDAN BUAH SEGAR KELAPA SAWIT (Elaeis guineensis Jacq.)." Jurnal Agro Fabrica 1, no. 2 (December 16, 2019): 62–70. http://dx.doi.org/10.47199/jaf.v1i2.106.

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Physical damage to fruit on oil palm fresh fruit bunches due to harvest and postharvest activities include injured fruit mesocarp, fruit weight loss, and loss in fruit bunches. One of the postharvest handlings that can maintain the physical quality of the fruit is by giving gibberellins (GA3). This research aims to determine the effect of the concentration of gibberellins (GA3) whit application time before harvest on the physical quality of postharvest oil palm fresh fruit bunches. The research was conducted at Afdeling VI Garden Cot Girek PT. Perkebunan Nusantara I and in the Cot Girek Palm Oil Factory laboratory. The time of the research was conducted in June until July 2019. The study used a non factorial randomized block design method with 4 treatment levels (0 mg/l, 50 mg/l, 100 mg/l, and 150 mg/l) with 3 replications. The administration of gibberellins has a significant effect on fruit loss and fruit weight reduction with the best concentration of gibberellins is 50 ppm but does not have a significant effect on pH, moisture content, and fruit free fatty acids.
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Rizky, Sarah Afrita, Mardhiah Hayati, and Marai Rahmawati. "INISIASI PEMBENTUKAN BUAH MENTIMUN (Cucumis sativus L.) VARIETAS MERCY F1 SECARA PARTENOKARPI AKIBAT KONSENTRASI GIBERELIN DAN DOSIS PUPUK KALIUM." Jurnal Ilmiah Mahasiswa Pertanian 6, no. 3 (August 1, 2021): 1–8. http://dx.doi.org/10.17969/jimfp.v6i3.17559.

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Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi giberelin dan dosis pupuk kalium, serta interaksi keduanya terhadap pertumbuhan dan hasil tanaman mentimun varietas Mercy F1 secara partenokarpi. Penelitian ini dilaksanakan di lahan kebun Gampong Ilie-Ulee Kareng-Banda Aceh, Laboratorium Fisiologi Tumbuhan dan Laboratorium Hortikultura Fakultas Pertanian Universitas Syiah Kuala. Rancangan yang digunakan adalah Rancangan Acak Kelompok pola faktorial 4 x 3 dengan 3 ulangan. Faktor yang diteliti yaitu konsentrasi giberelin (Kontrol, 100, 200 dan 300 ppm) dan dosis pupuk kalium (Kontrol, 100 dan 200 kg ha-1). Hasil penelitian menunjukkan bahwa konsentrasi giberelin berpengaruh sangat nyata terhadap jumlah biji per buah dan bobot buah per tanaman. Hasil mentimun terbaik dijumpai pada konsentrasi giberelin 300 ppm dalam menurunkan jumlah biji per buah. Hasil penelitian juga menunjukkan bahwa dosis pupuk kalium berpengaruh sangat nyata terhadap bobot buah per tanaman dan bobot buah per buah, berpengaruh nyata terhadap jumlah buah per tanaman dan panjang buah per buah. Hasil mentimun terbaik dijumpai pada dosis 200 kg ha-1 pupuk kalium dalam meningkatkan bobot buah per buah dan panjang buah per buah. Interaksi antara konsentrasi giberelin dan dosis pupuk kalium berpengaruh nyata terhadap jumlah buah per tanaman dan jumlah biji per buah. Hasil mentimun terbaik dijumpai pada kombinasi konsentrasi giberelin 300 ppm dengan dosis pupuk kalium 100 kg ha-1.(Parthenocarp Fruit Initiation of Cucumber (Cucumis sativus L.) Mercy F1 Variety due to the Concentration of Gibberellins and the Dose of Potassium Fertilizer)Abstract. This study aims to determine the effect of gibberellins concentration and potassium fertilizer dosage, as well as their interaction on the growth and yield of Mercy F1 cucumber plant by parthenocarpy. This research was carried out in the gardens of Gampong Ilie-Ulee Kareng-Banda Aceh, Plant Physiology Laboratory and Horticultural Laboratory, Faculty of Agriculture, Syiah Kuala University. The design used was a Randomized Factorial Block Design 3 x 4 with 3 replications. The factors observed were the concentration of gibberellins (Control, 100, 200 and 300 ppm) and the dose of potassium fertilizer (Control, 100 and 200 kg ha-1). The results showed that the concentration of gibberellins had a very significant effect on the number of seeds by fruit and fruit weight by plant. The best cucumber yields were found at a concentration of 300 ppm gibberellins in reducing the number of seeds by fruit. The results also showed that the dose of potassium fertilizer had a very significant effect on fruit weight by plant and fruit weight by fruit, significantly affected the number of fruits by plant and fruit length by fruit. The best cucumber yield was found at a dose of 200 kg ha-1 of potassium fertilizer in increasing fruit weight by fruit and fruit length per fruit. The interaction between the concentration of gibberellins and the dose of potassium fertilizer significantly affected the number of fruits by plant and the number of seeds by fruit. The best cucumber yields were found in a combination of 300 ppm gibberellin concentration with a dose of 100 kg ha-1 potassium fertilizer.
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Roberts, A. V., P. S. Blake, R. Lewis, J. M. Taylor, and D. I. Dunstan. "The Effect of Gibberellins on Flowering in Roses." Journal of Plant Growth Regulation 18, no. 3 (November 1999): 113–19. http://dx.doi.org/10.1007/pl00007058.

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31

Strack, Z., R. Karwowska, and E. Kraszewska. "The effect of several stress conditions and growth regulators on photosynthesis and translocation of assimilates in the bean plant." Acta Societatis Botanicorum Poloniae 44, no. 4 (2015): 567–88. http://dx.doi.org/10.5586/asbp.1975.052.

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Studies were performed on young bean plants, grown in water culture. The effect of salt stress, X-flays and flooding on growth, photosynthesis and translocation of assimilates was investigated. Salt stress (NaCl and Na<sub>2</sub>SO<sub>4</sub>), especially at - 4.5 atm. of water potential, depressed all the mentioned processes, but most dramatically - photosynthesis. Export of photosynthetes from the blades decreased. Salt stress not only reduced the rate of translocation, but also influenced the pattern of <sup>14</sup>C-distoibution, especially inhibited transport to apical part, with growth seriously retarded. Gibberellin (GA<sub>3</sub>, 100 ppm sprayed on leaves) counteracted the negative effects caused by salinization, but did not affected either photosynthesis, or translocation in plants from normal nutrient solution. The conclusion may be advanced, that salt stress disturbed the balance of plant hormones especially gibberellins, which probably participate in. regulation of assimilate translocation.
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Nowak, Tomasz J. "Effect of gibberellin, auxin and kinetin treatments combined with foliar applied NPK on the yield of Capsicum annuum L. fruits and their capsaicin content." Acta Agrobotanica 33, no. 1 (2013): 81–92. http://dx.doi.org/10.5586/aa.1980.007.

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The Wrocław version of hydroponic culture was applied. Under optimal conditions of root fertilization the plants were sprayed with growth regulators sueh as gibberellins, auxins and kinetins, and .their mixtures. Each growth regulator treatment was applied with or without NPK added. The influence of these treatments on the fresh and dry weight of the fruit, percentage of ripe fruits and content and yield of capsaicin was studied. The highest yield of fruits and capsaicin. was obtained from plants sprayed with gibberellic acid and kinetin (in concentrations of 10 and 5 mg/l, respectively) together with NPK foliar application. No influence of ,growth regulators and foliar-applied NPK was noted on capsaicin content and dry weight of fruits.
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Weidner, Stanisław. "Role of gibberellins and cytokinins in regulation of germination during development and ripening of Triticale caryopses." Acta Societatis Botanicorum Poloniae 53, no. 2 (2014): 257–70. http://dx.doi.org/10.5586/asbp.1984.024.

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The germination of caryopses of M-T3 <em>Triticale</em> generation, which were freshly harvested in different growth and developmental phases has been studied. A significant influence of the abscisic acid (ABA) accumulation on the increment of number of germinating caryopses has been found. Already in the first phase af the embryogenesis considerable stimulating effects of kinetin and gibberellin-A<sub>3</sub> (GA<sub>3</sub>) on the germination of embryos which were isolated from freshly collected grains have been shown. When both stimulators were used together marked synergetic reaction occurred. It has been also determined that in the initial period of embryogensis premature germination occurs, to a higher extent, under the action of cytokinins than! Whether in the further phases of the caryopse development, when embryo develop mainly through the cell elongation, mostly gibberellins seem to be responsible for the activation of germination processes. The more mature were seeds the quicker germinated whole caryopses and embryos isolated from them at different ripeness, after 3-month storage. The highest stimulation of germination by phytohormones has been found for the most mature caryopses. The action of gibberellic acid has been particulary strong.
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Vâtcă, Sorin Daniel, Ștefania Gâdea, Roxana Vidican, Mignon Șandor, Vlad Stoian, Anamaria Vâtcă, Adrian Horvath, and Valentina Ancuța Stoian. "Primary Growth Effect of Salix viminalis L. CV. Inger and Tordis in Controlled Conditions by Exploring Optimum Cutting Lengths and Rhizogenesis Treatments." Sustainability 14, no. 15 (July 28, 2022): 9272. http://dx.doi.org/10.3390/su14159272.

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The major disadvantage of setting up a willow coppice is the low survival rate, which reduces economic efficiency and crop sustainability. The aim of this research was to test, under controlled conditions, the impact of water, gibberellic acid A3 (0.05%), and humic acid (0.2%) on the growth and development of two willow clones. Under humic acid treatment, 20 cm cuttings of the Tordis clone developed up to 15 roots, and 25 cm cuttings developed more than 23. In comparison, water stimulated more than 15 roots for both 20 and 25 cm cuttings. Gibberellins acted as an inhibitor, especially on the roots, and the cuttings dried out from the top to the middle, with weak development of shoots and callus formation. For both clones, the highest number of active buds was observed on 20 and 25 cm cuttings grown in water, with more than four for Inger and more than seven for Tordis. Root development of the Inger clone had a maximum of eight for 25 cm cuttings grown in water; it was three times lower in the same variant of Tordis and two times lower for the Tordis clone with humic acid treatment. In general, Inger cuttings of 15 and 25 cm highlighted a delayed root formation when humic acids and gibberellins were applied. In controlled condition experiments, the Tordis clone was more suitable owing to its higher development and increased growth stability.
35

Gosai, Sanjeevan, Subash Adhikari, Saugat Khanal, and Padam Bahadur Poudel. "Effects of plant growth regulators on growth, flowering, fruiting and fruit yield of cucumber (Cucumis sativus L.): A review." Archives of Agriculture and Environmental Science 5, no. 3 (September 25, 2020): 268–74. http://dx.doi.org/10.26832/24566632.2020.050306.

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This review provides a comprehensive overview of the basic and applied aspects of different plant growth regulators in the regulation of growth and development of cucumber plants. The study is completely based on the use of secondary sources of data; related journals, government institutes, and relevant reports. Foliar application of PGRs has been shown to change the physiological and developmental processes, including plant vegetative growth, sex expression, yield, and yield components in cucumber. There are basically two types of growth regulators; plant growth promoters such as auxin, gibberellins, cytokinins, maleic hydrazide, ethephon, etc. and plant growth inhibitors such as ethylene, abscisic acids, dormins, etc. The combined use of auxins and gibberellins result in increased secondary growth. Maleic hydrazide (MH) along with Ethephon at 100 ppm each increases the number of nodes and primary branches. Ethrel at 300-400 ppm retards the secondary development and increase femaleness, and at 200-300 ppm make fruit surface smooth. Silver nitrate (AgNO3) at 400 ppm enhances the maleness in cucumber. Application of Ethephon at 300 ppm reduces the harvesting time of the fruit. Salicylic acid (at 2 doses of 0.07 mm/l + 0.18 mm/l) increases chlorophyll content and its exogenous application increases the fruit yield. Maleic Hydrazide (MH) alone at 100 ppm increases the femaleness, inhibits apical growth at 50-100 ppm, and increases fruit size at 200 ppm. Therefore, various auxin [indole-3-acetic acid (IAA), NAA], auxin transport inhibitor (TIBA), cytokinins (KIN), gibberellin [gibberellic acid (GA3)], ABA, ethylene [(2-chloroethylphosphonic acid (ethrel; ethephon; CEPA)] and growth retardant (MH) have been applied to control the vegetative growth and to maximize yield of cucumber. Numerous obstacles have hindered the quality cucumber production in Nepal; like environmental stresses, biotic and abiotic constraints, pest and disease outbreaks, and many others. The use of exogenous plant growth regulators has been crucial to Nepali cucumber producers as plant growth regulators has hasty effect on vegetative as well as the quality yield of plants. This study aims to reveal the suitable concentrations for the applications of growth regulators so that the use of such regulators is environmentally and toxicologically safe for both plants and the consumers.
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Kurnia Sari, Fransisca Mareta, Maria Marina Herawati, Endang Pudjihartati, and Martinus Andree Wijaya Setiawan. "THE EFFECT OF CONCENTRATION AND FREQUENCY OF GIBERELLINS ON THE GROWTH OF ARTEMISIA ANNUA Linn." Agric 34, no. 1 (August 18, 2022): 15–22. http://dx.doi.org/10.24246/agric.2022.v34.i1.p15-22.

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Artemisia is one of the plants that can be used as an alternative medicine for malaria. The Artemisia plant contains secondary metabolites in the form of artemisinin compounds that function as antimalarials. One of the efforts that can be done to increase the growth of Artemisia annua. plants is by using a growth regulator of gibberellins (GA3). Using a Randomized Block Factorial Designs (RBFD) with the first factor being the concentration of 50 mg.L-1 (K1), mg.L-1(K2), 150 mg.L-1 (K3), 200 mg.L-1 (K4). The second factor has 3 frequencies, 1 time giving 7HSPT (F1), 2 times giving 7HSPT and 14HSPT (F2), and 3 times giving 7HSPT, 14HSPT and 30HSPT (F3). In addition, there were plants that were not given GA3 control (without treatment). Parameters observed were plant height, leaf area, glandular trichome density, and plant dry weight. The results showed that the administration of gibberellins (GA3) with different concentrations and frequencies on plant height, leaf area, glandular trichome density, and dry weight of A. annua plants had no significant effect. Plants have not been able to grow properly because the provision of gibberellins that have not been active stimulates all plant tissues and directly seeps through the roots, stems and leaves.
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Susilo, Hadi, Nisa Rachmania Mubarik, and Triadiati Triadiati. "Characterization of Gibberellin Producing Rhizobacteria Isolated from Soil Forest in Banten." Current Biochemistry 2, no. 1 (April 20, 2015): 32–41. http://dx.doi.org/10.29244/cb.2.1.32-41.

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Gibberellin is plant growth regulator that stimulates cell elongation, seed germination, flowering, and fruit ripening. This study was conducted to isolate, identify, and optimize growth media for gibberellins producing- rhizobacteria isolated from rhizosphere soil of “keruing“ (Dipterocarpus sp.) tree in forest research Carita, Pandeglang, Banten. Eight bacterial isolates were obtained and all produced gibberellin. The BC2 isolate produced the highest of gibberellin (0.897 mg mL-1) and then selected for identification based on physiology, molecular character, and effects of growth media with variation of temperature, pH, and light. The result of physiological test indicated that BC2 isolate does not produce indole, positive on urease and oxidative carbohydrate. The phylogenetic analysis showed that BC2 isolate is belonged to Stenotrophomonas maltophilia with 98% similarity level. The optimation of growth media indicated that the growth of BC2 isolate was optimum at 30°C, pH 7, and dark condition.
38

Rodríguez-Ortiz, Roberto, M. Carmen Limón, and Javier Avalos. "Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants." Applied and Environmental Microbiology 75, no. 2 (December 1, 2008): 405–13. http://dx.doi.org/10.1128/aem.01089-08.

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ABSTRACT The fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces metabolites of biotechnological interest, such as gibberellins, bikaverins, and carotenoids. Gibberellin and bikaverin productions are induced upon nitrogen exhaustion, while carotenoid accumulation is stimulated by light. We evaluated the effect of nitrogen availability on carotenogenesis in comparison with bikaverin and gibberellin production in the wild type and in carotenoid-overproducing mutants (carS). Nitrogen starvation increased carotenoid accumulation in all strains tested. In carS strains, gibberellin and bikaverin biosynthesis patterns differed from those of the wild type and paralleled the expression of key genes for both pathways, coding for geranylgeranyl pyrophosphate (GGPP) and kaurene synthases for the former and a polyketide synthase for the latter. These results suggest regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this fungus. Expression of gene ggs1, which encodes a second GGPP synthase, was also derepressed in the carS mutants, suggesting the participation of Ggs1 in carotenoid biosynthesis. The carS mutations did not affect genes for earlier steps of the terpenoid pathway, such as fppS or hmgR. Light induced carotenoid biosynthesis in the wild type and carRA and carB levels in the wild-type and carS strains irrespective of nitrogen availability.
39

Saini, Hargurdeep S., Pawan K. Bassi, J. Stephen Goudey, and Mary S. Spencer. "Breakage of Seed Dormancy of Field Pennycress (Thlaspi arvense) by Growth Regulators, Nitrate, and Environmental Factors." Weed Science 35, no. 6 (November 1987): 802–6. http://dx.doi.org/10.1017/s0043174500079376.

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Effects of ethylene, gibberellins, kinetin, nitrate, light, and temperature, each alone or combined with other factors, were studied on the germination of dormant seed of field pennycress (Thlaspi arvenseL. # THLAR). Treated seed were incubated at a range of constant temperatures, at temperatures alternating daily between 12 and 22 C (12 h each), or at 12 C followed by transfer to 22 C. No germination ocurrred in water under any conditions. When applied in the dark KNO3had no effect on germination under any of the temperature regimes. A combination of KNO3, light, and a shift in temperature from 12 to 22 C induced 100% germination. Four days of incubation at 12 C induced maximal seed sensitivity towards KNO3+ light, and the sensitivity remained unchanged if the incubation at 12 C was extended to 15 days. Upon transfer to 22 C, the effect of low-temperature incubation was lost within 3 days. Of the two seed lots studied, one had a relatively lower chilling requirement, but both had similar requirements for light and KNO3. Gibberellic acids4+7(GA4+7) induced 100% germination in the dark or light at all temperature regimes. Gibberellic acid3(GA3) was promotive under isothermal conditions, but its effectiveness was enhanced by a shift in temperature from 12 to 22 C. Kinetin or ethephon, each applied alone, had little effect on germination. Ethephon and GA3together induced 100% germination.
40

Huarte, Héctor R., María del R. Pereyra Zorraquín, Eric M. Bursztyn, and María L. Zapiola. "Effects of Environmental Factors on Seed Germination and Seedling Emergence of Common Teasel (Dipsacus fullonum)." Weed Science 64, no. 3 (September 2016): 421–29. http://dx.doi.org/10.1614/ws-d-15-00136.1.

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Common teasel is an invasive and widespread weed in Argentina. Germination experiments were carried out from 2011 to 2014 to determine the effect of various environmental factors on germination and emergence. Germination of recently dispersed seeds was 12% in darkness at constant temperature. In contrast, seed exposure to light and alternating temperatures enhanced germination to 95%. The requirement of light and alternating temperatures suggest that common teasel has physiological dormancy. Several experiments were carried out to determine whether (1) seed responses to light and alternating temperatures have a hormonal basis, (2) seed coats can suppress germination, and (3) time and thermal conditions during seed storage reduce light and alternating temperature requirements. Germination was reduced (≤ 13%) by a gibberellin synthesis inhibitor. In contrast, the presence of gibberellins and an abscisic acid synthesis inhibitor increased germination to 95 and 38%, respectively. Results suggest that a higher ratio among gibberellins and abscisic acid (GA/ABA) leads to a break in dormancy. Germination was 100% when embryos were excised, suggesting that seed coats may suppress germination by mechanical restriction. Likewise, germination was enhanced by hydrogen peroxide (70%). This compound is known to increase GA/ABA ratio in agreement with a hormonal control of dormancy proposed for common teasel. An increment of storage time reduces light and alternating temperature requirements, allowing seeds to germinate in darkness. Taking these results together confirms that common teasel has physiological dormancy. Seedling emergence was progressively reduced from 70 to 8% by increased burial depth from 0 to 3 cm. Information from these experiments may facilitate development of effective management for common teasel.
41

Foschi, María Laura, Mariano Juan, Bernardo Pascual, and Nuria Pascual-Seva. "Influence of Seed-Covering Layers on Caper Seed Germination." Plants 12, no. 3 (January 18, 2023): 439. http://dx.doi.org/10.3390/plants12030439.

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Caper is a perennial shrub that is widespread in the Mediterranean Basin. Although the fruits contain many seeds, they germinate slowly and with very low percentages, due to their nondeep physiological dormancy. The influence of the testa and endosperm, as well as the effect of applying gibberellic acid (GA3) solutions on seed germination to release its dormancy, are reported in this study. The mechanical resistance exerted by the testa and endosperm against radicle protrusion in mature caper seeds was measured. The best germination results were obtained with seeds devoid of testa wetted with water and with intact seeds wetted with a 500 mg L−1 GA3 solution, without statistical differences between them. The GA3 addition triggers an increase in both the content of endogenous gibberellins (GA) and the GA/abscisic acid ratio, increasing germination. Its germination consists of two temporally separated events: testa cracking and endosperm piercing. Testa cracking begins in the hilum-micropillar area; it involves a signal from the embryo, which GA can replace, possibly by increasing the growth potential of the embryo. After testa cracking, the radicle emerges through a hole in the micropylar endosperm. The puncture force necessary to pierce the micropylar endosperm decreased drastically during the first day of imbibition, remaining practically constant until testa cracking, decreasing afterwards, regardless of the addition or not of gibberellins.
42

Rood, Stewart B., Darla M. Bruns, and Susan J. Smienk. "Gibberellins and sorghum development." Canadian Journal of Botany 66, no. 6 (June 1, 1988): 1101–6. http://dx.doi.org/10.1139/b88-157.

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Sorghum (Sorghum bicolor (L.) Moench) hybrids adapted for grain (cv. Northrup King X8102) or forage production (cv. Pride PF70) were grown in field trials at Lethbridge, Alta., Canada, and shoot cylinders containing the apical meristems were harvested at various developmental stages for analyses of endogenous gibberellin-like (GA-like) substances. Extracts were purified and chromatographed on sequential SiO2 partition columns and C18 reversed-phase high-performance liquid chromatography, and GA-like substances were detected and quantified by the cv. Tan-ginbozu dwarf rice microdroop assay. Concentrations of GA-like substances in the shoots were higher during vegetative growth than during shoot elongation or panicle elongation and the pattern of declining concentration of GA-like substances was observed for both hybrids over both field seasons. The qualitative distribution of GA-like substances was generally similar across hybrids and developmental stages, with a GA19-like substance accounting for about 60% of the total GA-like activity (GA19 is a native GA of sorghum). The exogenous application of GA3 promoted height growth and inhibited tillering, with maximal effects occurring when application was early, during rapid vegetative growth. Applications of GA3 subsequent to panicle initiation promoted panicle elongation but did not alter the time of anthesis. These results on endogenous GA concentration and response to exogenous GA3 application suggest a role for endogenous GAs in the control of shoot growth and development in sorghum.
43

Deligios, Paola A., Emma Rapposelli, Massimiliano G. Mameli, Limbo Baghino, Gian Mario Mallica, and Luigi Ledda. "Effects of Physical, Mechanical and Hormonal Treatments of Seed-Tubers on Bud Dormancy and Plant Productivity." Agronomy 10, no. 1 (December 25, 2019): 33. http://dx.doi.org/10.3390/agronomy10010033.

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The aim of this study was to develop a technique easy to apply in order to induce seed-tuber dormancy breakage. Over a two-year study, more than seven dormancy-breaking treatments were tested through evaluating different temperature effects alone or combined with gibberellins application, cutting in half of seed-tubers, and early haulm killing. Three varieties per year were considered: Spunta and Monalisa (medium and long dormancy) in both years, Europa during the first year and Arinda during the second year (both characterized by a short dormancy period). We found firstly that Europa and Arinda promptly responded to thermal treatments, and secondly to the same thermal treatments in combination with the application of gibberellins. Although not easily applicable, especially when a large volume of seed-tubers has to be handled (seed-tuber producers), the cutting in half of the seed-tubers also had a satisfactory result. Notwithstanding that treatments did not perfectly overlap between the two experiments, results were qualitatively similar. Therefore, these findings allow us to conclude that treatment with post-harvest storage at 20 °C, followed by a treatment with gibberellic acid at 38 days from harvesting, is the most efficient in releasing dormancy, in ensuring a good vegetative growth and productive performance at field-level irrespective of the variety.
44

Shan, Fuxin, Rui Zhang, Jin Zhang, Chang Wang, Xiaochen Lyu, Tianyu Xin, Chao Yan, Shoukun Dong, Chunmei Ma, and Zhenping Gong. "Study on the Regulatory Effects of GA3 on Soybean Internode Elongation." Plants 10, no. 8 (August 23, 2021): 1737. http://dx.doi.org/10.3390/plants10081737.

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Excessive plant height is an important factor that can lead to lodging, which is closely related to soybean yield. Gibberellins are widely used as plant growth regulators in agricultural production. Gibberellic acid (GA3), one of the most effective active gibberellins, has been used to regulate plant height and increase yields. The mechanism through which GA3 regulates internode elongation has been extensively investigated. In 2019 and 2020, we applied GA3 to the stems, leaves, and roots of two soybean cultivars, Heinong 48 (a high-stalk cultivar) and Henong 60 (a dwarf cultivar), and GA3 was also applied to plants whose apical meristem was removed or to girded plants to compare the internode length and stem GA3 content of soybean plants under different treatments. These results suggested that the application of GA3 to the stems, leaves, and roots of soybean increased the internode length and GA3 content in the stems. Application of GA3 decreased the proportion of the pith in the soybean stems and primary xylem while increasing the proportion of secondary xylem. The apical meristem is an important site of GA3 synthesis in soybean stems and is involved in the regulation of stem elongation. GA3 was shown to be transported acropetally through the xylem and laterally between the xylem and phloem in soybean stems. We conclude that the GA3 level in stems is an important factor affecting internode elongation.
45

Vargas-Hernandez, J.-Jesus, and Jeniffer-Ivette Vargas-Abonce. "Effect of giberellic acid (GA4/7) and girdling on induction of reproductive structures in Pinus patula." Forest Systems 25, no. 2 (July 20, 2016): e063. http://dx.doi.org/10.5424/fs/2016252-09254.

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Aim of study: Seed production in forest tree species commonly takes a long time due to the length of the juvenile stage. Even though several treatments have been used to induce early flowering in conifer species, experience on their use in subtropical Pinus species is limited. This study aimed to evaluate the effect of GA4/7 dose (0, 1.27 and 2.54 mg cm-2) and application time (July-October), alone or in combination with partial stem girdling, on male and female strobili production in young Pinus patula Schiede ex Schltdl. et Cham. clones. Area of study: Nine clones with different flowering background of high-elevation Pinus patula growing in a six-year old seed orchard established in Central México (Aquixtla, Puebla) at 2,800 m elevation.Material and methods: Two independent flowering trials (FT1 and FT2) were carried out in the seed orchard during the 2009 and 2010 flowering cycles; similar factors were evaluated at both trials but time of application, clones tested, and experimental design used varied for each of them. Partial stem girdling was done at the base of the trunk and the GA4/7 solution was injected into the xylem above the point of girdling. The following spring, the percentage of trees with strobili and the number of strobili per tree were determined for both male and female structures.Main results: Significant differences (p ≤ 0.05) among clones in flowering capacity were found at both trials. None of the treatments applied in FT1 resulted in an increase of strobili formation, most probably because they were applied too late in the growing season. In FT2, however, application of GA4/7 combined with partial stem girdling increased the percentage of trees with strobili and the number of strobili of both sexes, particularly when applied in early July. Partial stem girdling was more effective on promoting male strobili than female ones in gibberellin-treated grafts.Research highlights: Timing of GA4/7 application and stem girdling was important, with a significant interaction with clones tested in FT2 on production of male strobili but not on female strobili. Application of 1.27 mg cm-2 GA4/7 and stem girdling in July promoted the highest percentage of trees with strobili, and increased 25-fold the number of female strobili and 5-fold the number of male strobili per tree as compared to the control treatment. Thus, operational use of this induction treatment would be valuable to increase and accelerate seed production in Pinus patula seed orchards in the region.Keywords: early flowering; gibberellins; juvenile stage; seed orchard; seed production.Abbreviations: GA: Gibberellic acid; GA4: Gibberellic acid 4; GA7: Gibberellic acid 7; GA4/7: Mix of gibberellic acids 4 and 7; FT1: Flowering trial carried out in the 2009 season; FT2: Flowering trial carried out in the 2010 season.
46

Kulikowska-Gulewska, Halina, and Jan Kopcewicz. "The involvement of gibberellins in phytochrome-controlled flowering of Pharbitis nil." Acta Societatis Botanicorum Poloniae 71, no. 1 (2014): 23–27. http://dx.doi.org/10.5586/asbp.2002.003.

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The seedlings of <em>Pharbitis nil</em>, a sesitive short-day plant (SDP), were cultivated under special photoperiodic conditions: 72-h-long darkness, 24-h-long white light with low intensity, 24-h-long inductive night. During 24-h-long inductive darkness the total content of gibberellins in cotyledons underwent fluctuations with a maximum at 0 h and 8 h, and a decrease at the end of the dark period. FR light applied at the end of the 24-h-long white-light period inhibited flowering. R light flash and partially exogenous GA3 added on cotyledons could reverse the effect of FR. The seedling growth was not affected by FR and R light irradiation, but was promoted by exogenous GA3 application. The obtained results suggest that gibberellins are involved in photoperiodic control of SDP <em>P. nil</em> flowering. This involvement has nothing in common with participation of gibberellins in the control of the elongation growth of seedlings.
47

Grbic, Mihailo, Dragana Skocajic, Matilda Djukic, Danijela Djunisijevic-Bojovic, Dragica Obratov-Petkovic, and Ivana Bjedov. "Breaking of Judas tree seed dormancy by plant hormone treatments." Bulletin of the Faculty of Forestry, no. 109 (2014): 73–84. http://dx.doi.org/10.2298/gsf1409073g.

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The study deals with the influence of growth regulators on the germination of Judas tree (Cercis siliquastrum L.) seed which is double dormant. We simultaneously tested seeds prepared in the conventional procedure: scarification + stratification and scarified seeds treated with phytohormones from the groups of gibberellins, auxin and cytokinins. The results indicate a positive effect of gibberellic acid (GA), as well as some combinations of this phytohormone with others. Recommendations for practice are to combine the conventional procedure with GA. The procedure may shorten the duration of stratification; the application of GA should follow stratification because the temperature of 4?C does not provide growth regulators activity. The study results can serve as the base for easier generative reproduction of this valuable woody ornamental species which could have a wide use in changed climate conditions.
48

Ferrante, Antonio, Anna Mensuali-Sodi, and Giovanni Serra. "Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers." Open Life Sciences 4, no. 4 (December 1, 2009): 461–68. http://dx.doi.org/10.2478/s11535-009-0039-8.

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AbstractPlant hormones such as cytokinins and gibberellins are able to inhibit leaf yellowing in different species of cut flowers and potted plants. These hormones can be used alone or in combination among them for preserving chlorophyll in floriculture items. In the present study thidiazuron was tested alone or combined with GA3 for delaying leaf yellowing of cut stock flowers during vase life. Cut flowers were placed in a controlled environment and treated for 24 hours with the following solutions: distilled water (control) or solutions containing 5, 10 µM thidiazuron (TDZ), 0.5 mM gibberellic acid (GA3), or a combination of 0.5 mM GA3 with 5 µM TDZ. The effect of treatments was evaluated by measuring chlorophyll content, ethylene production, leaf gas exchanges and chlorophyll a fluorescence. Results showed that TDZ was able to delay leaf yellowing in light during whole experimental period (30 days). The effect of TDZ on dark stored flowers was less effective, and also delayed chlorophyll losses for 10–12 days. TDZ and GA3 combination did not show any synergistic nor beneficial effect. Gas exchange values such as net photosynthesis, vapour pressure deficit, stomatal conductance and water use efficiency were higher in the TDZ only treatment.
49

Loveys, BR, and M. Jusaitis. "Stimulation of Germination of Quandong (Santalum acuminatum) and Other Australian Native Plant Seeds." Australian Journal of Botany 42, no. 5 (1994): 565. http://dx.doi.org/10.1071/bt9940565.

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Intact seeds of quandong (Santalum acuminatum), Ixodia achillaeoides, Ptilotus exaltatus, Codonocaupus pyramidalis and Exocarpos aphyllus which are normally difficult to germinate responded to vacuum infiltration with gibberellins. In most cases GA4 was far more effective than GA3. Cytokinins, ethylene and silver ions did not affect quandong germination but abscisic acid (ABA) was inhibitory. If imbibed quandong seeds were subject to a rapid drying and re-wetting cycle, subsequent germination was inhibited. This transient drying was associated with a rise in endogenous ABA and the effect of the drying could be overcome by gibberellin. There was no correlation between ABA level and state of dormancy in recently imbibed seed suggesting that ABA was not necessary for the maintenance of dormancy. These results suggest that the mechanisms controlling quandong seed germination are hormonally based although the mechanical constraints imposed by the rigid shell may also be important.
50

Michniewicz, M., B. Rożej, and J. Stopińska. "The influence of nitrogen nutrition on the dynamics of growth and metabolism of endogenous growth regulators in Scotch pine (Pinus silvestris L.) seedlings." Acta Societatis Botanicorum Poloniae 45, no. 4 (2015): 495–510. http://dx.doi.org/10.5586/asbp.1976.044.

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Pine seedlings were cultivated in sterile agar cultures containing nitrogen as NH4Cl. The most pronounced positive effect on the growth of seedlings was affected by N used at a concentration of 50 ppm. After 4 months was stated that nitrogen had only a slight effect on elongation, of .shoots but decreased the length of roots. Nitrogen increased the lenght and number of primary and secondary needles as well as the fresh and dry matter of sboots. It stimulated also the number of lateral roots and the fresh and dry matter of the root system. Stimulation of shoot growth and differentiation as a result of nitrogen treatment was correlated with the increase -of free gibberellins and auxins and decrease of the amount of bound gibberellins and ABA-like inhibitor in shoots. However the effect of N on growth of roots was connected with the increase of auxins, cytokinins and ABA-like inhibitor in these organs.

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