Готові списки джерел за темами / ECPMV

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Добірка наукової літератури з теми "ECPMV"

Автор: Grafiati

Опубліковано: 11 березня 2023

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Статті в журналах з теми "ECPMV"

Alonso-Miguel, Daniel, Guillermo Valdivia, Diego Guerrera, Maria Dolores Perez-Alenza, Stanislav Pantelyushin, Angela Alonso-Diez, Veronique Beiss, et al. "Neoadjuvant in situ vaccination with cowpea mosaic virus as a novel therapy against canine inflammatory mammary cancer." Journal for ImmunoTherapy of Cancer 10, no. 3 (March 2022): e004044. http://dx.doi.org/10.1136/jitc-2021-004044.

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BackgroundInflammatory mammary cancer (IMC), the counterpart of human inflammatory breast cancer (IBC), is the deadliest form of canine mammary tumors. IMC patients lack specific therapy and have poor outcomes. This proof-of-principle preclinical study evaluated the efficacy, safety, and effect on survival of neoadjuvant intratumoral (in situ) empty cowpea mosaic virus (eCPMV) immunotherapy in companion dogs diagnosed with IMC.MethodsTen IMC-bearing dogs were enrolled in the study. Five dogs received medical therapy, and five received weekly neoadjuvant in situ eCPMV immunotherapy (0.2–0.4 mg per injection) and medical therapy after the second eCPMV injection. Efficacy was evaluated by reduction of tumor growth; safety by hematological and biochemistry changes in blood and plasma; and patient outcome by survival analysis. eCPMV-induced immune changes in blood cells were analyzed by flow cytometry; changes in the tumor microenvironment were evaluated by CD3 (T lymphocytes), CD20 (B lymphocytes), FoxP3 (Treg lymphocytes), myeloperoxidase (MPO; neutrophils), Ki-67 (proliferation index, PI; tumor cell proliferation), and Cleaved Caspase-3 (CC-3; apoptosis) immunohistochemistry.ResultsTwo neoadjuvant in situ eCPMV injections resulted in tumor shrinkage in all patients by day 14 without systemic adverse events. Although surgery for IMC is generally not an option, reduction in tumor size allowed surgery in two IMC patients. In peripheral blood, in situ eCPMV immunotherapy was associated with a significant decrease of Treg+/CD8+ ratio and changes in CD8+Granzyme B+ T cells, which behave as a lagging predictive biomarker. In the TME, higher neutrophilic infiltration and MPO expression, lower tumor Ki-67 PI, increase in CD3+ lymphocytes, decrease in FoxP3+/CD3+ ratio (p<0.04 for all comparisons), and no changes in CC-3+ immunostainings were observed in post-treatment tumor tissues when compared with pretreatment tumor samples. eCPMV-treated IMC patients had a statistically significant (p=0.033) improved overall survival than patients treated with medical therapy.ConclusionsNeoadjuvant in situ eCPMV immunotherapy demonstrated anti-tumor efficacy and improved survival in IMC patients without systemic adverse effects. eCPMV-induced changes in immune cells point to neutrophils as a driver of immune response. Neoadjuvant in situ eCPMV immunotherapy could be a groundbreaking immunotherapy for canine IMC and a potential future immunotherapy for human IBC patients.

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Albakri, Marwah M., Veronique Beiss, Steven N. Fiering, Nicole F. Steinmetz, and Scott F. Sieg. "Activation of human immune cells with cowpea mosaic virus." Journal of Immunology 202, no. 1_Supplement (May 1, 2019): 136.7. http://dx.doi.org/10.4049/jimmunol.202.supp.136.7.

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Abstract Novel approaches to retrieve and enhance immunity against cancer antigens have been established. One promising approach involves the use of virus particles derived from the plant virus, Cowpea Mosaic Virus (CPMV). Injection of CPMV into tumor stimulates systemic antitumor immunity in tumor mouse models and canine patients with tumors. The molecular pathways that account for these effects are not well defined. In these studies, we examine the capacity of CPMV to activate human monocytes. Peripheral blood mononuclear cells were incubated overnight with CPMV and CD14+ monocytes were examined by flow cytometry for expression of various markers. Cells were also incubated with “empty” (RNA-free) CPMV (eCPMV) for comparison. Responses to CPMV were also tested with THP-1 dual reporter cells, which are activated by various surface and cytoplasm pattern recognition receptors ligands except for TLR7. CPMV, but not eCPMV, mediated activation of human monocytes as indicated by induced expression of CD86, PDL-1, HLA-DR, IL-15R, and IP-10. In contrast to positive controls, PAM3CSK4 (TLR1/2 agonist) and LPS (TLR4 agonist), neither CPMV nor eCPMV induced activation of THP-1 cells. Activation of monocytes by CPMV was markedly inhibited by a chemical inhibitor of spleen tyrosine kinase (SYK), a key signaling component of TLR activation pathways. Effects of SYK inhibition were also observed in cells stimulated by a TLR7 agonist, imiquimod, but with different outcomes than CPMV stimulation, depending on the activation index that was assessed. These data demonstrate that CPMV activates monocytes in a manner that is highly dependent on RNA and SYK signaling. Overall, these data raise the possibility that TLR7 may play a role in CPMV adjuvant-like activity.

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Valdivia, G., D. Alonso-Miguel, A. Alonso-Diez, V. Beiss, S. Fiering, N. Steinmetz, M. D. Perez-Alenza, H. Arias-Pulido, and L. Peña. "ECPMV Neoadjuvant Immunotherapy Induced Neutrophilic Infiltration and Increased Survival in Canine Inflammatory Mammary Cancer." Journal of Comparative Pathology 191 (February 2022): 23. http://dx.doi.org/10.1016/j.jcpa.2021.11.053.

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Kerstetter-Fogle, Shukla, Wang, Beiss, Harris, Sloan, and Steinmetz. "Plant Virus-Like Particle In Situ Vaccine for Intracranial Glioma Immunotherapy." Cancers 11, no. 4 (April 10, 2019): 515. http://dx.doi.org/10.3390/cancers11040515.

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Despite aggressive multi-modality treatment with surgery, radiation and chemotherapies, malignant glioma inevitably recurs and has dismal survival rates. Recent progress in immunotherapy has led to a resurgence of interest, and immunotherapies are being investigated for treatment of glioma. However, the unique brain anatomy and a highly immunosuppressive glioma microenvironment pose significant challenges to achieving efficacy. Thus, there is a critical need for assessment of next-generation immunotherapies for glioma. In this study, we have investigated the efficacy of the nanoparticle platform technology based on plant-derived Cowpea mosaic virus like particles (empty CPMV or eCPMV) to instigate a potent immune response against intracranial glioma. CPMV immunotherapy has been shown to efficiently reverse the immunosuppressive tumor microenvironments in pre-clinical murine models of dermal melanoma and metastatic melanoma, metastatic breast cancer, intraperitoneal ovarian cancer and in canine patients with oral melanoma. In the present study, we demonstrate that in situ administration of CPMV immunotherapy in the setting of glioma can effectively recruit unique subset of effector innate and adaptive immune cells to the brain parenchyma while reducing immune suppressive cellular population, leading to regression of intracranial glioma. The in situ CPMV nanoparticle vaccine offers a potent yet safe and localized immunotherapy for intracranial glioma.

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Gee, Christine, Alison Small, Kathleen Shorter, and Wendy Y. Brown. "A Radiographic Technique for Assessment of Morphologic Variations of the Equine Caudal Cervical Spine." Animals 10, no. 4 (April 12, 2020): 667. http://dx.doi.org/10.3390/ani10040667.

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Equine Caudal Cervical Morphologic Variation (ECCMV) is a congenital malformation of the caudal cervical spine distinct from the more commonly recognized Cervical Vertebral Stenotic Myelopathy (CVSM). The most common presentation of ECCMV is recognized on the sixth cervical vertebra (C6). In “normal” presentations, the transverse processes on the left and right sides have a caudal lamina projecting ventrally below the caudal vertebral body in a heel shape. With ECCMV, variations occur to the structure of the caudal ventral lamina on one or both sides of C6 and may include the seventh cervical (C7) and first thoracic (T1) vertebrae and ribs, in varying configurations. Whereas the prevalence of ECCMV is not known, it has been recognized for many years and has been reported to occur with relatively high frequency within multiple populations of domesticated horses. To date, there is no documented link between the occurrence of ECCMV and clinical signs. However, based on retrospective studies, multiple authors have recognized the potential impact on performance that this condition may have. Establishing a reliable radiographic protocol for the consistent diagnosis of ECCMV would allow quantitative, scientific evaluation of the problem and support clinicians working in this field. We present a radiographic technique, which has been illustrated by diagnosis of ECCMV in three horses and confirmation of the diagnoses in two cases via postmortem examination.

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Greenwood, Sarah, Betty Chow-Lockerbie, Sophie Ramsauer, Garrett Wachoski-Dark, Cameron Knight, and Bruce Wobeser. "Prevalence of Equus caballus Papillomavirus Type-2 Infection and Seropositivity in Asymptomatic Western Canadian Horses." Veterinary Pathology 57, no. 5 (August 19, 2020): 632–41. http://dx.doi.org/10.1177/0300985820941270.

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Equus caballus papillomavirus type 2 (EcPV-2) has been recognized as a potential cause of a subset of genital squamous cell carcinomas (SCCs) in horses. In the current study, we measured EcPV-2 seropositivity in 50 healthy horses from Western Canada, and these were compared to a herd of horses with known EcPV-2 exposure. Second, the presence of EcPV-2 DNA was measured using EcPV-2-specific PCR (polymerase chain reaction), performed on a variety of tissues collected at necropsy from 70 horses that lacked any history, gross, or histologic evidence of neoplasia or papillomavirus-associated disease. EcPV-2-specific RNA in situ hybridization (R-ISH) was performed on PCR-positive samples to identify the specific tissues infected. The prevalence of asymptomatic infection with EcPV-2 in Western Canadian horses was 20/70 (29%). Exposure to EcPV-2 as measured by seropositivity was 18/50 (36%). EcPV-2 positivity by anatomic location, as measured by R-ISH, was as follows: penis 10/29 (35%), vulva 5/34 (15%), eyelid 8/68 (12%), oral mucosa 7/65 (11%), skin from muzzle 7/68 (10%), and retropharyngeal lymph node 2/64 (3%). The youngest horses with EcPV-2 infection, based on PCR, were fetuses, suggesting for the first time that vertical transmission of EcPV-2 occurs in horses. The current study observed an increased prevalence of EcPV-2 as compared to previous studies. We suggest that this difference is due to our use of biopsies in place of superficial swabs. We propose that EcPV-2 infection in asymptomatic horses is more common than previously reported and that the virus’ role in equine genital SCCs may be more complex than originally thought.

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Greenwood, Sarah, Betty Chow-Lockerbie, Tasha Epp, Cameron Knight, Garrett Wachoski-Dark, Valerie MacDonald-Dickinson, and Bruce Wobeser. "Prevalence and Prognostic Impact of Equus caballus Papillomavirus Type 2 Infection in Equine Squamous Cell Carcinomas in Western Canadian Horses." Veterinary Pathology 57, no. 5 (August 19, 2020): 623–31. http://dx.doi.org/10.1177/0300985820941266.

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Equus caballus papillomavirus type-2 (EcPV-2) has been proposed as a causal factor in equine genital squamous cell carcinoma (SCC). This study had 2 objectives: first, calculate the frequency of papillomavirus (PV) and EcPV-2 infection in papillomas, carcinomas in situ (CIS), and SCCs in Western Canadian horses; and second, determine if EcPV-2 status of equine SCCs is associated with overall survival (OS). EcPV-2 status of 115 archived tissue samples, spanning 6 years, was determined using broad spectrum (MY09/11) and EcPV-2-specific polymerase chain reaction (PCR) assays, EcPV-2-E6/E7 chromogenic RNA in situ hybridization (R-ISH), and amplicon sequencing. A retrospective survey gathered data on history, outcome, breeding, treatment, and rationales of referring veterinarians when managing PV-associated diseases. Histologic grade and completeness of surgical margins of SCCs were also considered. EcPV-2 DNA was identified in 10/58 (17%) SCC, 8/27 (30%) papillomas, 0/5 CIS, and 0/11 lesions identified as “other.” Overall, 18/101 (18%) of these lesions were positive for EcPV-2. EcPV-2 was identified in 10/35 (29%) SCCs arising from genital tissues but in 0/22 SCCs from other locations. There was no association between breeding history and EcPV-2 status of genital SCCs. EcPV-2 status of genital SCCs was not associated with OS ( P = .76). The strongest negative predictors of OS were a lack of treatment ( P < .01) and recurrence post-treatment ( P < .01). Weaker predictors of OS included older age at time of diagnosis ( P = .02). Completeness of margins at surgical excision, concurrent disease, treatment type, anatomic location of the SCC (anogenital vs other), and histologic grade of the SCC did not influence OS ( P > .1).

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Mira, Juliana, Mariana Herman, Luiza S. Zakia, Giovane Olivo, João P. Araújo, Alexandre S. Borges, and José P. Oliveira-Filho. "Frequency of Equus caballus papillomavirus in equine aural plaques." Journal of Veterinary Diagnostic Investigation 30, no. 4 (March 30, 2018): 565–68. http://dx.doi.org/10.1177/1040638717753495.

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Despite the reported association between aural plaques and the presence of Equus caballus papillomavirus (EcPV), there are few data regarding the distribution of viral types in different geographic regions or possible correlations for different papillomaviruses and lesion characteristics. We detected the presence and frequency of EcPV (1–7) DNA in aural plaque biopsies of horses from different regions of Brazil and identified the patterns of these infections or coinfections and their possible association with lesion severity. A total of 108 aural plaque biopsies from horses in the 5 geopolitical regions of Brazil were examined. We performed PCR to detect EcPV DNA in the biopsies. At least 1 type of EcPV was detected in 97% of the samples. EcPV coinfection was observed in 59% of the samples. Compared to the other viruses, EcPV-4 was found at the highest frequency in coinfection (84%) or individually identified (32%). EcPV-2 and -7 were not detected. No significant association was found between lesion characteristics (type and distribution) and either the viral type detected or the presence of coinfection. EcPV is widely distributed in Brazil, both isolated and in coinfection; the viral type does not appear to influence the clinical characteristics of equine aural plaques.

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Alloway, Elizabeth, Keith Linder, Susan May, Trevor Rose, Josepha DeLay, Susan Bender, Alison Tucker, and Jennifer Luff. "A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus–2 Infection." Veterinary Pathology 57, no. 3 (March 17, 2020): 427–31. http://dx.doi.org/10.1177/0300985820908797.

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Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As Equus caballus papillomavirus–2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.

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Abdullah, Hikmat Najem, and Ibrahim Fahmi Ali. "Design of Chaotic Bifurcation Parameter Modulation Scheme using Mean Value Estimation." Iraqi Journal of Information & Communications Technology 1, no. 1 (May 14, 2018): 52–61. http://dx.doi.org/10.31987/ijict.1.1.13.

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In this paper, the bifurcation parameter of the chaotic map is estimated accurately by utilizing the ergodic properties of a chaotic dynamical signal. Binary Phase Shift Keying Ergodic Chaotic Parameter Modulation (BPSK-ECPM) scheme is used to modulate the information signal in the bifurcating parameter of the chaotic map. A mean value estimation technique is used at the receiver to retrieve the original information accurately. This method minimizes the computational complexity of the receiver; thereby, reduces the total manufacturing cost. Simulation results confirm that, in AWGN channel and at bit error rate (BER) of 10-3, BPSK-ECPM achieves gains in Eb/N0 of about 0.7dB and 5dB in comparison with conventional direct sequence spread spectrum with BPSK modulation (DS-SS BPSK) and conventional ergodic chaotic parameter modulation spread spectrum (ECPM-SS) systems respectively. The results also confirm that in Rayleigh fading channel and at BER of 10-3, BPSK-ECPM achieves gains in Eb/N0 of about 6.3dB and 1.6dB in comparison with DS-SS BPSK and ECPM-SS systems respectively, which make BPSK-ECPM an ideal candidate for the intelligent transportation system (ITS).

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Більше джерел

Дисертації з теми "ECPMV"

Danzi. "eCPMV NANOPARTICLES: THE POTENTIAL OF A BIO-INSPIRED STRATEGY FOR PLANT PROTECTION." Doctoral thesis, 2021. http://hdl.handle.net/11562/1044775.

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Agricultural yield is dramatically affected by crop pests and pathogens, which are considered a major threat to food security. The intense and indiscriminate use of pesticides led to an increase in resistance occurrence in microorganisms and environmental issues, leading to the awareness towards the need for new alternative strategies respectful of both health and environment. Nanotechnology is one of the most promising technologies of the modern era. Indeed, its multidisciplinary nature makes it fascinating and exploitable in many fields, precisely including agriculture. Nanoagrochemicals can indeed provide a valuable alternative to common pesticides, due to a more targeted and enhanced efficacy, resulting in a lower dosage of active substances and a reduction of possible off-target effects. Although the biosafety of nanoagrochemicals is still not completely understood, their development for plant protection represents a promising strategy to achieve a sustainable and environmentally friendly crop production system. Plant viruses can play an important role in this context since they are naturally occurring proteinaceous nanoparticles able to carry, protect, and deliver a cargo. Moreover, the biosafety concerns about the use of plant viruses for crop protection have been exceeded by the development of nucleic acid-free virus nanoparticles, named empty virus-like particles (eVLPs). The Cowpea mosaic virus (CPMV) can be produced as eVLPs (eCPMV) through transient expression in Nicotiana benthamiana plants, at high titres and in an inexpensive manner. Additionally, eCPMV can be functionalized with peptides that can be displayed on the external surface by genetic manipulation or chemical conjugation, thus offering a wide range of possibilities for their application in plant protection. In this work, eCPMV nanoparticles have been exploited in an attempt to develop new products applicable for crop protection, using different approaches that rely on the physical stability of the eCPMV capsid to expose natural bioactive peptides or on the capacity of the empty eCPMV cavity to load natural molecules: (i) eCPMV as a scaffold for antimicrobial peptide (AMP) exposure, (ii), eCPMV as a plant immunity-triggering nanoparticle, and (iii) eCPMV as a functional nanocarrier.While it has not been possible to produce eCPMV harbouring AMPs on the surface, eCPMV functionalized with different elicitors were successfully expressed and purified and showed the capacity to induce plant molecular defence responses. Moreover, eCPMV nanoparticles were loaded with different molecules, efficiently further released in apoplastic-mimicking conditions, thus establishing the possibility for the cargo to be delivered within plant tissues. Overall, the data enforce to reconsider the paradigms regarding eCPMV functionalization, in particular in terms of peptide features required for genetical modification. Moreover, they support that eCPMV is a promising tool to develop new nanobiopesticides applicable in agriculture. Finally, they unveil unexpected effects of eCPMV nanoparticles, such as their capacity to disturb fungal growth and to fully protect plant from bacterial infection, which will certainly deserve attention for future research

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Книги з теми "ECPMV"

Canada Mortgage and Housing Corporation. and Canadian Centre for Housing Technology., eds. Effects of ECPM furnace motors on electricity and gas use. [Ottawa]: Canada Mortgage and Housing Corporation, 2005.

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Частини книг з теми "ECPMV"

Halappanavar, M., and R. Mukkamala. "ECPV: EFficient Certificate Path Validation in Public-Key Infrastructure." In Data and Applications Security XVII, 215–28. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/1-4020-8070-0_16.

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Thurmayr, Rudolf, and Bernd Graubner. "Towards a European Classification of Procedures in Medicine (ECPM) — AIM-SESAME and the Dutch and German Approach —." In Medical Informatics Europe 1991, 775–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-93503-9_139.

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Тези доповідей конференцій з теми "ECPMV"

Smith, M., T. Portone, and L. Swiler. "Effects of Fracture Transmissivity Relationship on Repository Performance Characteristics." In 3rd International Discrete Fracture Network Engineering Conference. ARMA, 2022. http://dx.doi.org/10.56952/arma-dfne-22-0007.

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Abstract When simulating flow and transport through sparsely fractured rocks in Earth’s subsurface, discrete fracture network (DFN) modeling has become the alternative approach to continuum approaches. In this work, DFNs are generated using dfnWorks, a parallelized computational suite developed by Los Alamos National Laboratory. The DFNs are then mapped to an equivalent continuous porous medium (ECPM) to allow for nuclear waste repository performance assessment simulations. To this end, reactive flow and transport calculations are performed in PFLOTRAN, a parallel multiphase flow and reactive transport code. For this study, the application problem is a crystalline repository reference case based on the Forsmark site in Sweden. When converting the fracture networks into ECPMs, fracture transmissivity is used to determine the continuum permeability field. New capabilities in dfnWorks have enabled the use of the depth-dependent correlated relationship. To understand the effect of adding the depth-dependent relationship, this study compares repository performance quantities of interest and ECPM permeabilities from the same DFN where the only change is the transmissivity relationship. It was found that although the permeabilities for the varying transmissivity relationships were significantly different, this did not strongly influence the quantities of interest.

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Wasef, Albert, Yixin Jiang, and Xuemin Shen. "ECMV: Efficient Certificate Management Scheme for Vehicular Networks." In IEEE GLOBECOM 2008 - 2008 IEEE Global Telecommunications Conference. IEEE, 2008. http://dx.doi.org/10.1109/glocom.2008.ecp.129.

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Paplicki, Piotr. "Simplified reluctance equivalent circuit for hybrid excited ECPMS-machine modelling." In 2016 21st International Conference on Methods and Models in Automation and Robotics (MMAR). IEEE, 2016. http://dx.doi.org/10.1109/mmar.2016.7575140.

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