Дисертації з теми "E1 and E2"
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Hudson, Natalia Joanna. "Analysis of diversity of hepatitis C virus glycoproteins E1 and E2." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12644/.
Повний текст джерелаSorathia, Rina. "Identification of human papillomavirus type 16 (HPV16) E1^E4 binding partners and the characterisation of the E1^E4/E2 interaction." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446481/.
Повний текст джерелаCocquerel, Laurence. "Caracterisation des domaines transmembranaires des glycoproteines e1 et e2 du virus de l'hepatite c." Paris 6, 2001. http://www.theses.fr/2001PA066052.
Повний текст джерелаAlmeida, Ana Isabel Matos de. "Produção de partículas semelhantes a retrovírus como candidatas a vacina para a Hepatite C." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/9297.
Повний текст джерелаVírus da Hepatite C (HCV) infeta mais de 170 milhões de pessoas em todo o mundo, sendo uma das principais causas de cancro do fígado. Como tal, é extremamente importante e desejável a criação de uma vacina. Recentemente têm vindo a ser desenvolvidas e comercializadas vacinas baseadas em partículas semelhantes a vírus (VLPs) capazes de estimular respostas humorais e celulares eficientes. Os objetivos deste trabalho consistiram no melhoramento de linhas celulares produtoras de VLPs derivadas de retrovírus e no melhoramento da qualidade das partículas virais, com o intuito de produzir VLPs candidatas a vacina para a hepatite C. Para tal, utilizaram-se duas metodologias. Um sistema de troca de cassette (RMCE), que é um sistema versátil e rápido para o desenvolvimento de linhas celulares, permitindo obter uma elevada expressão de um gene de interesse, tendo sido utilizado para expressar os epítopos do HCV. Testaram-se dois sistemas, um mediado pela enzima Cre e outro mediado pela enzima Flipase. Para o melhoramento da qualidade das partículas utilizou-se a metodologia de silenciamento por RNAi para eliminar a incorporação da tetraspanina CD63, uma vez que a presença desta proteína nas VLPs poderá desencadear respostas imunológicas inespecíficas. Verificou-se que o sistema de troca de cassette mediado pela enzima Cre não foi eficiente nas condições experimentais utilizadas, não tendo sido possível produzir VLPs pseudotipadas com os epítopos do HCV. No sistema mediado pela enzima Flipase a troca de cassette foi eficiente obtendo-se clones que expressam as proteínas E1 e E2, para a produção de VLPs pseudotipadas com o envelope do vírus HCV. Em relação ao silenciamento da tetraspanina CD63 apesar de serem testadas diversas condições, a percentagem de silenciamento obtida foi muito reduzida e instável, concluindo-se não ser possível realizar o silenciamento desta proteína através das sequências utilizadas.
Patel, Janisha. "An investigation of the complexes formed between the hepatitis C virus E1 and E2 glycoproteins." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342004.
Повний текст джерелаMeunier, Jean-Christophe. "Étude des glycoprotéines E1 et E2 du virus de l'hépatite C : influence de la glycosylation de la protéine E1 sur la formation du complexe E1E2." Lille 1, 1999. https://pepite-depot.univ-lille.fr/LIBRE/Th_Num/1999/50376-1999-23.pdf.
Повний текст джерелаEn second lieu, nous avons identifié les sites potentiels de glycosylation reconnus sur la glycoprotéine E1 du virus de l'hépatite C. Nous avons montré que seul le site de glycosylation n°5 de la protéine E1 n'est pas utilisé pour l'addition d'un oligosaccharide. Puis nous avons étudié le rôle des glycannes dans le repliement et l'assemblage des glycoprotéines E1 et E2. Nous avons montré que la glycosylation sur le site n°4 de la protéine E1 est un élément déterminant pour la formation d'un complexe E1E2 natif, alors que la mutation des sites 1, 2 ou 3 n'entraîne que peu ou pas de perturbations. Les hypothèses permettant d'expliquer l'importance de la glycosylation sur le site n°4 ont été testées. Enfin, nous avons testé la sécrétion de formes tronquées de la protéine E1, délétées leurs extrémités C-terminales. Nous avons pu mettre en évidence une forme tronquée très efficacement sécrétée. Une meilleure compréhension des mécanismes de formation des hétérodimères matures permettrait une optimisation de leur potentiel immunogénique. De même, des formes fortement sécrétées de la protéine E1 seraient très utiles pour constituer un outil diagnostique
Spieker, Mark-Christoph [Verfasser]. "The origin of low-lying collective E1 and E2 strength in atomic nuclei / Mark-Christoph Spieker." München : Verlag Dr. Hut, 2017. http://d-nb.info/1126297844/34.
Повний текст джерелаMarkson, Gabriel Benjamin. "A systematic analysis of the E1, E2, and E3 interactions within the human protein ubiquitination system." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612409.
Повний текст джерелаCiczora, Yann. "Rôles fonctionnels des domaines transmembranaires des glycoprotéines d'enveloppe E1 et E2 du virus de l'hépatite C." Lille 2, 2006. http://www.theses.fr/2006LIL2S064.
Повний текст джерелаHepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2 associated as heterodimers. These proteins are essential for virus infectivity. The two charged residues (Asp728 and Arg 730) of transmembrane domain (TMD) of E2 do not contribute equally in the glycoprotein functions. The two charged residues are required for ER retention, but only the aspartic acid is necessary for heterodimerization. Moreover the mutation of this charged residues affects the entry functions of these proteins. We have done a tryptophane scanning mutagenesis of each residue of these segments. The Asp728 and the two glycine residues (Gly354 and Gly358) are required for the formation of the heterodimer. Moreover other residues (Lys370, Leu726, Ala727, Ala729) are also implicated in these interactions. Finally, our observations indicate that the TMDs are also involved in virus entry. Indeed, some mutants of the TMDs of E1 and E2 affected an early step of the fusion between the viral and cell membrane
Brett, Tricia Korrin. "The fate of estrone (E1), 17beta-estradiol (E2), estriol (E3) and 17alpha-ethinylestradiol (EE2) in surface waters." Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/46253.
Повний текст джерелаSoranzo, Thomas. "Approches Recombinantes pour l’Etude Structure/Fonction des Protéines E1, E2 et p7 du Virus de l’Hépatite C." Thesis, Université Grenoble Alpes (ComUE), 2015. http://www.theses.fr/2015GREAV056.
Повний текст джерелаThe Hepatitis C virus (HCV) is a major cause of chronic liver disease, including cirrhosis and liver cancer. An estimated 170 million people worldwide are chronically infected with HCV and 3 to 4 million people are infected each year. One of the major handicaps of the HCV research is the lack of effective in vitro culture systems and animal models. To adress this issue, we chose a recombinant approach to study the E1, E2 and p7 proteins of HCV.The E1, E2 and p7 proteins are involved in critical steps of the viral cycle. They are membrane proteins, a class of protein that is extremely complex to express. Indeed, overexpression of membrane proteins is often toxic to the host cells. This phenomenon is caused by protein aggregation or degradation in the cytoplasm due to a lack of available membrane space for their integration into the host cell. Moreover, overexpression of membrane proteins induces saturation of the cellular machinery linked to membrane proteins. This diversion prevents the flow of a normal cell cycle and is fatal to the host cell. Destabilization of the host cell's membrane and its homeostatis may also be caused by the high concentration of membrane proteins or their heterologous nature. To circumvent these limitations, we used a method for producing membrane proteins in their native form by a cell-free system in the presence of liposomes; a technology patented by the University Joseph Fourier and licenced by the startup company Synthelis. First, we have set up the cell-free production system using a bacterial lysate from E. coli and a complementary energy mix. We then used this system to study the p7 viroporine. This protein is essential for the production of infectious virus particles and is involved in viral assembly making it an attractive therapeutic target. The production of a large quantity of p7 proteoliposomes allowed us to characterize the protein by biochemical and biophysical techniques. We have demonstrated the inhibition of oligomerization of p7 by HMA, which thereby inhibits its ion channel function. Thanks to the flexibility of the cell-free expression system we have characterized the structure of the viroporine within the membrane in a neutron reflectivity assay and have confirmed the funnel shape of the protein complex. Preliminary results on proteoliposomes E1E2 offer hope for the production viral particles mimicking the hepatitis C virus in order to better study the virus and fight against this epidemic.Together, these results confirm the suitability of the expression of membrane proteins in native forms using a cell-free system in the presence of liposomes. Proteoliposomes products are a new tool for the study of HCV and consideration for very broad therapeutic applications and the development of biopharmaceuticals based on the use of recombinant membrane proteins
Radtke, Christina [Verfasser], Birke Andrea [Akademischer Betreuer] Tews, Michael R. [Gutachter] Knittler, and Norbert [Gutachter] Tautz. "Charakterisierung der pestiviralen Glykoproteine E1 und E2 / Christina Radtke ; Gutachter: Michael Knittler, Norbert Tautz ; Betreuer: Birke Andrea Tews." Greifswald : Universität Greifswald, 2020. http://d-nb.info/1211087069/34.
Повний текст джерелаRadtke, Christina [Verfasser], Birke Andrea [Akademischer Betreuer] Tews, Michael [Gutachter] Knittler, and Norbert [Gutachter] Tautz. "Charakterisierung der pestiviralen Glykoproteine E1 und E2 / Christina Radtke ; Gutachter: Michael Knittler, Norbert Tautz ; Betreuer: Birke Andrea Tews." Greifswald : Universität Greifswald, 2020. http://d-nb.info/1211087069/34.
Повний текст джерелаHaddad, Juliano. "Etude biochimique et fonctionnelle de la glycoprotéine E1 du virus de l'Hépatite C (HCV)." Thesis, Lille 2, 2017. http://www.theses.fr/2017LIL2S029/document.
Повний текст джерелаBeing part of the viral particle, HCV envelope glycoproteins E1 and E2 play an essential role in virion morphogenesis as well as in HCV entry into liver cells. These glycoproteins form a non-covalent heterodimer, and until recently, research on HCV envelope glycoproteins has been mainly focused on E2. Indeed, this glycoprotein is the receptor-binding protein, it is also the major target of neutralizing antibodies and it was postulated to be the fusion protein. However, the recent publications of the structure of E2 do not show the presence of a fusion peptide and its structure does not fit with what one would expect for a fusion protein, suggesting that E1 alone or in association with E2 might be responsible for the fusion step. Concerning E1, only the crystal structure of the two-fifth N-terminal region, comprising amino acids 192 to 270, has been reported. This partial structure reveals a complex network of covalently linked, intertwined homodimers. The overall fold of the N-terminal E1 monomer consists of a beta-hairpin (β1 and β2) followed by a segment composed of a 16 amino-acid long alpha-helix (α1) flanking a three-strand antiparallel beta-sheet (β3, β4 and β5). In addition to the characterization of secondary structures within E1, a region located in the middle of the polypeptide (approximately between aa 274 and 292) has been suggested to play an active role during the fusion process and might potentially act as a fusion peptide. We took advantage of these recently published data to further investigate the functional role of HCV glycoprotein E1 by using a site-directed mutagenesis approach targeting conserved amino acids in the N-terminal region as well as in the region postulated to contain the fusion peptide in the context of an infectious clone. As expected, our results indicate that these mutations have no effect on virus replication. However, twenty-one out of twenty-eight mutations led to attenuation or inactivation of infectivity. Interestingly, two attenuated mutants, T213A and I262A, were less dependent on tight junction protein claudin-1, a co-receptor for HCV. Instead, these mutant viruses relied on another claudin (claudin-6) for cellular entry, indicating a shift in receptor dependence. In contrast, two other mutants, L286 and E303, were more dependent on claudin-1 for cellular entry into hepatoma cells cells. We also identified an interesting mutation downstream of the putative fusion peptide, G311A, which leads to the release of non-infectious particles having a defect in cellular entry. Finally, an unexpected phenotype was also observed for D263A mutant, which was no longer infectious but led to the secretion of viral particles devoid of genomic RNA. Further characterization of the D263A mutant revealed a change in subcellular co-localization between HCV RNA and E1, highlighting for the first time a crosstalk between HCV glycoprotein E1 and the genomic RNA during HCV morphogenesis.In conclusion, our observations allowed for the identification of specific regions in the E1 glycoprotein that play a role in virion assembly and entry, highlighting the major role played by this protein at different steps of the HCV infectious cycle
Liu, Bing. "Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition." Doctoral thesis, Universitat Autònoma de Barcelona, 2018. http://hdl.handle.net/10803/665314.
Повний текст джерелаUbiquitination and SUMOylation are of the most studied post-translational modifications (PTMs). Here, we focus on USP25, USP28, and the SUMO E1-E2 protein-protein recognition in these two PTM pathways. USP25 and USP28 have important roles in cellular processes, and their enzymatic activities are regulated by diverse PTMs including SUMOylation, ubiquitination, and phosphorylation. SUMO E1-E2 protein-protein interaction is a major discrimination step in the conjugation pathway. In this thesis, the main goals include the elucidation of the structural basis for the activity regulation of USP25 and USP28, as well as to decipher the structural determinants for the specificity provided by the E1 UFD-E2 interaction. We have solved the crystal structure of human USP25 (18 – 714). Unexpectedly, USP25 displays a homotetrameric quaternary assembly that is directly involved in the inhibition of its enzymatic activity, revealing a novel tetramerization/inhibition mechanism. In vitro biochemical and kinetic assays with dimer, tetramer and truncation constructs of USP25 support this mechanism, displaying in all cases a higher catalytic activity in the dimer assembly. Moreover, the strong stabilization of tankyrases in cultured cells by the ectopic expression of the USP25 dimer verifies the biological relevance of this novel tetramerization/inhibition mechanism. Regarding to the E1 UFD-E2 interaction, we have solved the crystal structure of the E1 UFD-E2 complex in both human and A. thaliana. Despite the low sequence homology displayed by the UFD binding interface, structural comparison between complexes reveals common determinants in the interfaces between human, yeast, and A. thaliana. Structural comparison also reveals a strong conservation in the E2 binding interface across species, despite the strong specificity displayed in SUMO conjugation assays for each organism. Interestingly, E2 residues outside the UFD interface had impact on SUMO conjugation, suggesting the contribution of determinants other than the primary UFD binding interface in the specificity of the conjugation system.
Stockhammer, Engelbert, and Özlem Onaran. "Accumulation, distribution and employment. A structural VAR approach to a Post-Keynesian Macro Model." Inst. für Volkswirtschaftstheorie und -politik, WU Vienna University of Economics and Business, 2002. http://epub.wu.ac.at/1220/1/document.pdf.
Повний текст джерелаSeries: Working Papers Series "Growth and Employment in Europe: Sustainability and Competitiveness"
Laurans, Françoise. "Definition histologique et approche moleculaire des interactions peupliers/rouilles (populus deltoides x p. Nigra cv. Ogy/melampsora larici-populina races e1 et e2)." Orléans, 1997. http://www.theses.fr/1997ORLE2017.
Повний текст джерелаOnaran, Özlem, and Engelbert Stockhammer. "Do profits affect investment and employment? An empirical test based on the Bhaduri-Marglin model." Inst. für Volkswirtschaftstheorie und -politik, WU Vienna University of Economics and Business, 2005. http://epub.wu.ac.at/1534/1/document.pdf.
Повний текст джерелаSeries: Working Papers Series "Growth and Employment in Europe: Sustainability and Competitiveness"
Uribe, Laplechade Catalina del Carmen. "Evaluación de la aplicación de estiércol animal en relación a la presencia, disponibilidad y biodisponibilidad de estrona (E1), 17β-estradiol (E2) y 17α-etinilestradiol (EE2) en suelos degradados". Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/151314.
Повний текст джерелаCon el objeto de buscar alternativas de producción más amigables con el medio ambiente, y evitar el deterioro de los ecosistemas, la producción silvoagropecuaria ha generado opciones sustentables y ecológicas como la agricultura orgánica, la cual conserva o aumenta la materia orgánica del suelo reciclando los residuos de cosecha, poda, estiércol y guano de animales, a través de distintos sistemas de incorporación al suelo. Según la Ley 20.089, el estiércol corresponde a fecas, orinas y productos de cama de animales, que no han sido compostado. El guano y la orina de los animales contienen cantidades importantes de nitrógeno, fósforo, potasio y otros elementos necesarios para el crecimiento de las plantas. La combinación de estiércol, paja de cereales y restos hortícolas es una mezcla de alta calidad como abono para el suelo. La composición de los guanos es muy variable y generalmente depende de la dieta que se le suministra al animal. La legislación chilena señala que la carga ganadera establecida se debe fijar considerando que no se debe sobrepasar el límite de 170 kg de nitrógeno·ha-1·año-1. Por otro lado, la ganadería intensiva crea problemas de depósito de estiércol y contaminación de agua. En este tipo de ganadería de producción intensiva el uso de fármacos u otros insumos es una práctica habitual que tiene como objetivos, aumentar la eficiencia en la engorda de los animales y la producción de leche. Uno de los problemas que puede provocar este hecho, es el aumento de la concentración de algunos contaminantes emergentes como lo son las hormonas estrogénicas naturales y sintéticas, por ejemplo, la estrona (E1), el 17β-estradiol (E2) y el 17α-etinilestradiol (EE2), que están consideradas disruptores endocrinos, y aunque se encuentren en muy bajas concentraciones, pueden provocar serios daños en animales y en seres humanos. Por esto, el objetivo de este trabajo fue evaluar la biodisponibilidad de dos hormonas naturales (E1 y E2) y una hormona sintética (EE2) en suelos restaurados con estiércol, empleando plantas de trigo como indicadores y compararlas con una extracción mediante hidroxipropil-β-ciclodextrina (HPCD). Para esto se evaluó la concentración de los tres analitos (E1, E2 y EE2) en estiércol de vacuno, cerdo y caballo, realizando una extracción asistida con ultrasonido, seguido de una etapa de clean up, derivatización y cuantificación en un cromatógrafo de gases acoplado a espectrometría de masas. También se realizó una caracterización física y química de los tres tipos de suelos y los distintos estiércoles, se determinó la fracción biodisponible en plantas de trigo cultivadas en suelos enmendados con estiércol y se estimó la concentración biodisponible mediante la extracción de las hormonas con ciclodextrina, para así validar este método como biosimulador a través de la comparación con los resultados obtenidos en el bioensayo con plantas de trigo. Finalmente, se realizó un estudio de degradación de las tres hormonas estrogénicas, aplicadas directamente a los suelos o agregadas a través del estiércol enriquecido con éstas, en dos periodos de tiempo, el primero durante treinta días y el segundo en siete días. Las hormonas naturales E1 y E2 se encontraron en los tres estiércoles, mientras que solo en el estiércol de cerdo se encontró la hormona sintética EE2. Los tres analitos se encontraron biodisponibles en las raíces de las plantas de trigo, siendo la EE2 en el suelo Codigua la que presentó mayor biodisponibilidad, el bioensayo con ciclodextrina correlacionó con la fracción biodisponible en las plantas de trigo, por lo tanto, el método biosimulador serviría como sistema predictivo en los tres suelos utilizados. Finalmente, el tiempo de degradación de las hormonas fue en general rápido, las tres hormonas se degradaron en su mayoría dentro de los dos primeros días, siendo la EE2 la más estable y la E2 la menos estable, ya que posiblemente ocurriría su degradación por oxidación a E1, por lo tanto, la concentración de E1 en las primeras horas tendería a aumentar
Intending to find environmentally production alternatives and avoid ecosystem damage, is that agro forestry production has generated sustainable and ecological alternatives as organic agriculture, which preserves or increases organic material from the ground by recycling harvest, pruning, manure and animal guano remains, all these through different ground incorporation systems. According to law no. 20.089, manure is composed by non composted feces, urine and animal bed products. Guano and urine from animals contains a significant amount of nitrogen, phosphor, potassium and some other elements needed for plants growth. The mixture made of manure, cereal straw and horticultural remains is a high quality fertilizer for the soil. Different guano compositions is very variable y generally depends on the animal diet. Current legislation points that established livestock load must be determinated considering not exceeding 170 kilograms limit of nitrogen·ha-1·year-1. On the other hand, intensive animal breeding creates manure deposit and water pollution problems. In this type of intensive animal breeding production, it is usual practice the use of drugs and other supplies that look for increase efficiency on animal fattening and milk production. One of the problems that this fact could cause is the increase on some emerging pollutants concentration as natural and synthetic estrogen hormones, like estrone (E1), 17β-estradiol (E2) y 17β-ethinylestradiol (EE2), which are considered as endocrine disruptors, that although are found on low concentration, can cause serious damage both in animals and in humans. Therefore, the main objective of this work was to evaluate the effect of the application of manure on soils and determine the bioavailable fraction of two natural hormones (E1 and E2) and a synthetic hormone (EE2) in wheat plants grown in the soils restored with manure, comparing the results with a bioassay using hydroxypropyl-β-cyclodextrin (HPCD) as an extractant. For this, the concentration of the three analytes (E1, E2 and EE2) in cow, pig and horse manure was evaluated, performing an assisted extraction with ultrasound, then a clean up, derivatization and measuring were made by in a coupled gas chromatograph to mass spectroscopy. A physical and chemical characterization of the three types of soils and the different manure was also carried out, the bioavailable fraction in wheat plants cultivated in soils amended with manure was determined and the bioavailable concentration was estimated by extracting the hormones with cyclodextrin, to validate this method as a biosimulator through the comparison with the results obtained in the bioassay with wheat plants. Finally, a study of degradation of the three estrogenic hormones, applied directly in the soil or added through the manure enriched with these, in two periods of time, the first during a month and the second for seven days. The natural hormones E1 and E2 were found in the three manures, and only synthetic mannitol EE2 was found in pig manure. The three analytes were found bioavailable in the roots of wheat plants with EE2 in Codigua soil having the highest bioavailability, the bioassay with cyclodextrin correlated with the bioavailable fraction in wheat plants, validating the biosimulator method as a predictive method, and finally, the time of degradation of hormones is generally fast, the three hormones are degraded mostly within the first two days, EE2 being the most stable and E2 the least stable, may because of this is degraded by oxidation to E1, therefore the concentration of E1 in the first hours tends to increase
Fondecyt
Greenlees, Paul Thomas. "Identification of excited states and evidence for octupole feformation in '2'2'6U." Thesis, University of Liverpool, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367435.
Повний текст джерелаAmorim, Kamila Pereira de. "Desenvolvimento de um método por ponto nuvem dos hormônios naturais E1 e E2 em amostras de urina e determinação por CLAE/EC utilizando eletrodo de diamante dopado com boro." Universidade Federal de Goiás, 2015. http://repositorio.bc.ufg.br/tede/handle/tede/5073.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Cloud point extraction method (CPE) was used for the determination of estrone (E1) and 17β-estradiol (E2) hormones in human urine. The combination of the electrochemical detection techniques with high-performance liquid chromatography (HPLC-EC) was used for the detection and quantification of these hormones. A boron doped diamond electrode (BDD) pretreated cathodically was used as electrode material for all electrochemical measurements. The optimized chromatographic parameters resulted in a mobile phase composition of KH2PO4 (0.01 mol L-1; pH 5.0) / ACN (72:28 V/V), flow 1.2 ml min-1. An applied potential for electrochemical detection of 1.0 V x Ag/AgCl (3.0 mol L-1) was selected from hydrodynamic voltammograms constructed for each hormone changing the potential between 0.3 V and 1.2 V x Ag/AgCl (3.0 mol L-1). Limits of detection (S/N = 3) of 500 ng mL-1 and limits of quantification of 800 ng mL-1 were obtained for both E1 and E2 hormones without any extraction process. Urine samples at pH 5.0 and 7.0 were investigated aiming the influence of pH on the efficiency of the CPE process, and the optimum results for the most current signal of the hormones was obtained at pH 7.0. Extractor solvent volumes were changed in the 0.5-2.5 mL range, and the optimum results were obtained when using 1.0 mL of Tergitol TMN-6 surfactant (10% aqueous solution). From the equation of the calibration curves obtained with and without the CPE procedure it was possible to determine the pre-concentration factor (FC) and all the other parameters involving the efficiency of CPE method. A comparison of the efficiency of CPE method with direct liquid-liquid extraction with the organic solvent CCl4 was carried out and the results showed that the CPE method was quite superior to liquid-liquid extraction. The validation of the method was carried out from intra-day recovery experiments and inter-day and evaluated the accuracy, precision and repeatability. The proposed method was applied to individual samples of urine of 1 man, 1 pregnant woman, 1 woman in fertile age, and 1woman in lactating stage. The values of the variation coefficients of the recovery percentages were lower than 15%.
Método de extração por ponto nuvem (EPN) foi usado para a determinação dos hormônios estrona (E1) e 17β-estradiol (E2) em urina humana. A combinação entre as técnicas de detecção eletroquímica e cromatografia líquida de alta eficiência (CLAE-EC) foi usada para a detecção e quantificação desses hormônios. Um eletrodo de diamante dopado com boro (DDB) pré-tratado catodicamente foi usado como material de eletrodo para todas as determinações eletroquímicas. Os parâmetros cromatográficos otimizados resultaram em uma composição de fase móvel de KH2PO4 (0,01 mol L-1; pH 5,0) / ACN (72:28 V/V) vazão de 1,2 mL min-1. Um potencial aplicado para a detecção eletroquímica de 1,0 V x Ag/AgCl (3 mol L-1) foi selecionado a partir de voltamogramas hidrodinâmicos construídos para cada hormônio variando-se o potencial entre 0,3 V e 1,2 V x Ag/AgCl (3,0 mol L-1). Limites de detecção (S/R = 3) de 500 ng mL-1 e limites de quantificação de 800 ng mL-1 foram obtidos para ambos os padrões dos hormônios E2 e E1 sem qualquer processo de extração. Amostras de urina em pH 5,0 e 7,0 foram investigadas quanto a influência do pH na eficiência do processo de extração, e o melhor resultado referente ao maior sinal de corrente dos hormônios foi obtido em pH 7,0. Os volumes de solvente extrator foram variados na faixa de 0,5-2,5 mL e o melhor resultado referente ao sinal de corrente dos hormônios foi obtido pelo uso de 1,0 mL de solução aquosa 10% do surfactante Tergitol TMN-6. A partir da equação da reta obtida das curvas analíticas, com e sem o procedimento de EPN, foi possível determinar o fator de pré-concentração (FC) e todos os demais parâmetros envolvendo a eficiência do método de EPN. Uma comparação sobre a eficiência dos métodos de EPN com extração direta líquido-líquido com o solvente orgânico CCl4 foi realizada e os resultados mostraram que método de EPN mostrou-se bastante superior a extração líquido-líquido. A validação do método foi feita a partir de ensaios de recuperação intra-dia e inter-dia, sendo avaliadas a exatidão, precisão e repetitividade. O método proposto foi aplicado em amostras individuais de urina de 1 homem, 1 mulher gestante, 1 mulher em idade fértil e 1 mulher lactante. Os valores dos coeficientes de variação das porcentagens de recuperação foram menores que 15%.
Moenne-Loccoz, Rémy. "Impact des glycoprotéines d'enveloppe E1 et E2 du virus de l'hépatite C sur la réponse au traitement antiviral interféron-a pégylé/ribavirine chez des patients atteints d'hépatite chronique C." Strasbourg, 2011. https://publication-theses.unistra.fr/public/theses_doctorat/2011/MOENNE-LOCCOZ_Remy_2011.pdf.
Повний текст джерелаThe standard of care (SOC) treatment, i. E. Pegylated interferon-alpha/ribavirin, is efficient in only 50% of patients chronically infected with hepatitis C virus (HCV)-genotype 1. The high variability of HCV E1/E2 envelope glycoproteins may indirectly contribute to viral resistance to treatment by selection of strains with high infectivity and/or increased ability to escape to immunity. This hypothesis was investigated by in silico and in vitro functional analyses (pseudoparticles HCVpp). Molecular signatures (MS) and covariant amino acid minimal networks, defined on E1/E2, were correlated with treatment response. Three out of the four MS which were defined and functionally assessed showed results concordant with the hypothesis. The nonresponse (NR)-related residues 431A and 642V led to a decrease in antibody (Ab)-mediated HCVpp neutralization using patients sera, a 431A or 642V-dependent increase of HCVpp infectivity at the entry step, and a 431A-dependent increase of interaction with CD81 and SR-BI. The response (R)-related residue 219T decreased HCVpp infectivity. Minimal networks of covariant amino acids separated NR-related from R-related strains and included three out of the four MS previously mentioned. Conclusion: Our results support an indirect contribution of HCV E1/E2 to treatment efficacy. NR-related 431A and 642V favour HCVpp infectivity with concomitant escape from neutralizing Ab while R-related 219T decreases HCVpp infectivity, suggesting that virus-host interactions during viral entry may be involved in the SOC treatment failure
Guimarães, Tatiane Sant\'Ana. "Detecção e quantificação dos hormônios sexuais 17 \'beta\'-estradiol (E2), estriol (E3), estrona (E1) e 17 \'alfa\'-etinilestradiol (EE2) em água de abastecimento: estudo de caso da cidade de São Carlos, com vistas ao saneamento ambiental." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-26022009-100015/.
Повний текст джерелаOne of the major problems of environmental engineering is the water contamination. The sanitary persons have been concerned with the gonadal hormones, notably the estrogen, biologically active compounds extremely, which have been referred to as etiologic agents of feminization and several types of cancers. The natural estrogen 17 \'beta\'-estradiol (E2), estriol (E3), estrone (E1) and synthetic 17 \'alpha\'-ethinylestradiol (EE2), developed for medical use of hormone replacement therapy in women and contraceptive methods, are those that attract larger concerns by the continuous introduction into the environment; hormones that have the best conformation recognized by receptors that result answers maximum, so they are considered responsible for most of the effects disruptors triggered by the wastewater disposal. The change in patterns on the sexual activity of young people and the concern with family planning, led to the large consumption of contraceptives that, in the urine, are led by the distribution net to water. The indiscriminate use of these hormones in cattle, pigs, poultry and aquaculture are responsible for part of this contaminant in the source. The hormones excreted in the urine and feces and agents from the food processing industries in the sanitary concern that the launch of effluents in nature or treated, are the main routes of contamination of the aquatic environment, either by lack of infrastructure, sanitation, or by inefficiency technological and/or operating in the removal of these compounds in the treatment plants, water or effluent. Despite having relatively short stocking-lige when compared to other organics such as pesticides, natural estrogens are continuously released into the environment, which gives them character cumulative. The proposal of this research was to verify the presence of estrogen in the gross water that arrives at the water treatment plant, after the treatment, in water treated by reverse osmosis and by Milli Q technology. To verify and quantify presence of these hormones estrogen in water supply of San Carlos-SP, examinations were conducted through immunoassay chemiluminescent and radioimunoassays. The results showed that the ETA has no efficient solution for removal of interest analytes of this research, because in the treated water were found values similar to crude water.
Morice, Yoann. "Le virus de l'hépatite C : études de la variabilité inter- et intra-génomique : production sous forme soluble de protéines membranaires impliquées dans l'interaction virus-cellule hôte." Paris 7, 2002. http://www.theses.fr/2002PA077217.
Повний текст джерелаKlein, Marina [Verfasser], Michael [Akademischer Betreuer] Roggendorf, Elke [Akademischer Betreuer] Cario, and Daniel [Akademischer Betreuer] Hoffmann. "Evolution of the envelope proteins E1 and E2 and of specific humoral immune response to these proteins in a group of patients infected by HCV in a single-source outbreak / Marina Klein. Gutachter: Elke Cario ; Daniel Hoffmann. Betreuer: Michael Roggendorf." Duisburg, 2011. http://d-nb.info/1015361803/34.
Повний текст джерелаSteiner, Laure D. "A Study of the fate and transport of estrogenic hormones in dairy effluent applied to pasture soils." Diss., Lincoln University, 2009. http://hdl.handle.net/10182/1306.
Повний текст джерелаTsai, Chia-hao, and 蔡佳豪. "Structural Characterization of HCV E1 and E2 Proteins." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/32252340507340326948.
Повний текст джерела慈濟大學
分子生物及細胞生物研究所
96
Abstract Hepatitis C virus (HCV) is an enveloped, positive-stranded RNA virus classified in the Hepacivirus genus of the Flaviviridae family. The HCV genome encodes three structural proteins: a capsid protein and two envelope glycoproteins, E1 and E2. E1 and E2 are thought to play pivotal roles at different steps of the HCV replicative cycle. There is now strong evidence that E1 and E2 are essential for host-cell entry, binding to receptor(s), inducing fusion with the host-cell membrane as well as assembling viral particle. E1 and E2 are type I transmembrane (TM) glycoproteins, with an N-terminal ectodomain and short C-terminal TM domain. These proteins interact with each other and assemble as noncovalent heterodimers. Like other viral envelope proteins involved in host-cell entry, HCV envelope proteins are thought to induce fusion between the viral envelope and a host-cell membrane. The HCV envelope glycoproteins E1 and E2 are thought to be class II fusion proteins because the putative fusion peptide is proposed to localize in an internal sequence linked by antiparallel β-sheets. We have prepared several constructs containing truncated E1 or E2. The truncated E1 or E2 protein can be expressed and purified from E. coli. Circular dichroism analysis of these expressed proteins showed little difference between pH 7 and 6. Only E1192~260 fragment (amino acid sequence 192 to 260) could induce liposome fusion at low pH, while other fragments could not. Thus, our data suggest that amino acid between sequence 232~260 play an role in the event of membrane fusion.
Whitehurst, Christopher Benton. "Structure and assembly of the sindbis virus E1 and E2 transmembrane proteins." 2006. http://www.lib.ncsu.edu/theses/available/etd-05112006-212104/unrestricted/etd.pdf.
Повний текст джерелаHu, Yan. "Interactions between papillomavirus E1 and E2 proteins and cellular replication factors modulate each others functions." 2006. http://proquest.umi.com/pqdweb?did=1140183911&sid=5&Fmt=2&clientId=39334&RQT=309&VName=PQD.
Повний текст джерелаTitle from PDF title page (viewed on Oct. 09, 2006) Available through UMI ProQuest Digital Dissertations. Thesis adviser: Melendy, Thomas. Includes bibliographical references.
Kajii, Takashi. "Long-term effects of prostaglandin E2 on the mineralization of a clonal osteoblastic cell line (MC3T3-E1)." Doctoral thesis, 1999. http://hdl.handle.net/2115/30170.
Повний текст джерелаProstaglandin (PG) E2 is thought to be a mediator of the effect of mechanical stress on bone formation, but its effects on osteoblasts have not yet been fully described. Here, the effects of the continuous application of PGE2 and indomethacin, an inhibitor of prostaglandin G/H synthase (cyclo-oxygenase), on the proliferation, differentiation and mineralization of a clonal osteoblastic cell line, MC3T3-E1, were investigated. The cells were cultured in media with either a high (1 μg/ml) or a low (1 ng/ml) concentration of PGE2, with indomethacin (1 μg/ml) and, as a control, with neither agent. The effects of PGE2 and indomethacin were assessed quantitatively. Indomethacin and a high concentration of PGE2 increased the total protein compared to the control and low-PGE2 cultures. 7 days after confluence, alkaline phosphatase (ALP) activity within the cells and extracellular matrices increased. This increase was highest with indomethacin and lowest with a high concentration of PGE2. ALP activity also increased in the medium, but only 21 days after confluence; the effects of the agents were similar to those on the cells and matrices. The accumulation of calcium, inorganic phosphate and hydroxyproline was highest with indomethacin. PGE2 production was at its maximum when the cells were at confluence and was inhibited by indomethacin. Specific [3H]PGE2 binding to the microsomal fraction of the cell was also measured to examine the expression of the PGE2 receptor. The amount of [3H]PGE2 binding per mg of protein was highest at confluence, then decreased and again increased in the mineralizing stage. These results suggest that indomethacin increases ALP activity and the accumulation of mineralized tissue in MC3T3-E1 cells, presumably by inhibiting the production of PGE2. PGE2 could signal the suppression of mineralization as early as confluence.
Hokkaido University (北海道大学)
博士
歯学
Mansky, Kim Carpenter. "Role of the bovine papillomavirus type E1 and E2 proteins in viral transcription and DNA replications." 1997. http://catalog.hathitrust.org/api/volumes/oclc/37900807.html.
Повний текст джерелаTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 178-198).
Pelzer, Christiane [Verfasser]. "Characterization of novel E1 and E2 enzymes and their role in ubiquitin and FAT10 conjugation / vorgelegt von Christiane Pelzer." 2009. http://d-nb.info/1011541882/34.
Повний текст джерелаFranken, Tobias [Verfasser]. "Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV-Strukturproteine core, E1 und E2 / vorgelegt von Tobias Franken." 2008. http://d-nb.info/990706370/34.
Повний текст джерелаClower, Randolph Vincent. "The papillomavirus E1 helicase and E2 protein bind to and stimulate the enzymatic functions of human topoisomerase I and DNA polymerase delta." 2006. http://proquest.umi.com/pqdweb?did=1184160381&sid=7&Fmt=2&clientId=39334&RQT=309&VName=PQD.
Повний текст джерелаTitle from PDF title page (viewed on Mar. 20, 2007) Available through UMI ProQuest Digital Dissertations. Thesis adviser: Melendy, Thomas E. Includes bibliographical references.
Schmidt, Matthias [Verfasser]. "Herstellung und Charakterisierung von virusähnlichen Partikeln auf der Basis von Fusionsproteinen aus HBV-Coreprotein und HCV-Oberflächenproteinen E1 bzw. E2 / vorgelegt von Matthias Schmidt." 2008. http://d-nb.info/998515760/34.
Повний текст джерелаCastelão, Cindy Duarte 1990. "Papel dos mecanismos envolvendo esteróides sexuais (E1 e E2), sua variação genética e biomarcadores circulantes na etiopatogenia de tumores ginecológicos (leiomiomas e cancro do colo do útero)." Master's thesis, 2013. http://hdl.handle.net/10451/10028.
Повний текст джерелаOs leiomiomas são neoplasias benignas que se formam a partir das células do músculo liso. Estes são os tumores mais comuns do aparelho reprodutor feminino. O cancro do colo do útero, cujo agente patogénico é o HPV, é o segundo cancro mais frequente e a segunda maior causa de morte nas mulheres em todo o mundo. Um dos factores de risco para o desenvolvimento de neoplasias em tecidos sensíveis a hormonas é a exposição, excessiva e cumulativa a estrogénios. Um factor importante da toxicologia dos estrogénios, para além da estimulação da proliferação de células epiteliais, é o seu metabolismo oxidativo. Assim, estudou-se polimorfismos funcionais nas seguintes enzimas: o CYP1A1 (rs4646903), a COMT (rs 4680), a MPO (rs2333227). Estudou-se igualmente a actividade da RTM e a concentração de estradiol circulante. Neste estudo observou-se que o alelo C, responsável pela maior actividade de CYP1A1, é um factor de risco em ambas as patologias. O alelo A, que concede menor actividade à enzima COMT, apresenta-se como um factor de risco para as patologias em estudo. Observámos que o genótipo GA da enzima MPO revelou-se um factor de risco em ambas as patologias – isto poderá indicar que esta é importante na eliminação do HPV; ou poderá ser um indício de que quantidades excessivas de ROS são prejudiciais às células transformadas. A RTM não demonstrou resultados estatisticamente significativos. Os níveis de estradiol circulante foram superiores nas populações patológicas corroborando a teoria que esta hormona é tumorigénica, quer directa quer indirectamente.A maior concentração de estradiol na população de leiomiomas pode indicar uma maior dependência hormonal deste. O facto dos resultados obtidos serem idênticos nos leiomiomas e cancro do colo do útero é interessante: são dois tumores diferentes, não só na sua etiologia, mas também na sua fisiologia; no entanto, os riscos não se distinguiram, indicando aparência em termos de susceptibilidade.
Leiomyomas are benign neoplasms that arise from smooth muscle cells. These are the most common tumors of the feminine reproductive system. HPV infection may lead to cervical cancer; this is the second most frequent cancer and the second biggest cause of death in women worldwide. One risk factor for the development of neoplasms in hormone-sensitive tissue in women is the excessive and cumulative exposure to estrogens. Estrogen toxicology can be explained by its hability to promote proliferation of epithelial cells, but also because of its oxidative metabolism. So, we studied the functional polymorphisms in the following enzymes: CYP1A1 (rs4646903), COMT (rs 4680), and MPO (rs2333227). We also studied the activity of RTM, and the concentration of estradiol. In this study we observed that the allele C, responsible for a more active CYP1A1, it’s a risk factor in both pathologies. The allele A, that codifies a lower activity COMT, also presents itself as a risk factor for both leiomyomas and cervical cancer. The GA genotype in the MPO enzyme revealed itself as a risk factor for both pathologies – which may indicate that MPO is important in the elimination of HPV from the organism; or that excessive amounts of ROS could lead to apoptosis of tumorigenic cells. The analysis of RTM activity demonstrated no significant statistical values. The levels of estradiol in blood were superior on the studied populations, supporting the theory that this hormone is tumorigenic, either acting directly or through its metabolism. The results obtained through this study demonstrate that both leiomyomas and cervical cancer show similar susceptibilities, even though they are very different from each other: not only in their etiology but also in their physiology.
Masavuli, Makutiro Ghislain. "Novel DNA Vaccine Formulations Against Hepatitis C Virus." Thesis, 2018. http://hdl.handle.net/2440/127111.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, Adelaide Medical School, 2018
Nevzorova, Yulia [Verfasser]. "Cell cycle regulation in the liver: differential functions of E-type cyclins E1 and E2 for G1/S-phase transition and endoreplication in mice / vorgelegt von Yulia Nevzorova." 2009. http://d-nb.info/1004859481/34.
Повний текст джерела