Дисертації з теми "Dynamic linking"
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Macrae, Robert. "Linking music metadata." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8837.
Повний текст джерелаAknuranda, Ismiarta. "An investigation into a framework of dynamic linking of relevant resources." Thesis, University of Manchester, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629576.
Повний текст джерелаSchäfer, Pierre-Alain. "Dynamic loading and linking native code on a real-time operating system." Instituto Tecnológico de Aeronáutica, 2007. http://www.bd.bibl.ita.br/tde_busca/arquivo.php?codArquivo=473.
Повний текст джерелаSanderson, Robert. "Linking past and future : an application of dynamic HTML for medieval manuscript editions." Thesis, University of Liverpool, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288863.
Повний текст джерелаKhachatryan, Knar. "Managing microfinance institutions : linking performance with service and capital portfolios." Phd thesis, Université Nice Sophia Antipolis, 2013. http://tel.archives-ouvertes.fr/tel-00911726.
Повний текст джерелаMartin, Benjamin. "Linking individual-based models and dynamic energy budget theory : lessons for ecology and ecotoxicology." Phd thesis, Universität Potsdam, 2013. http://opus.kobv.de/ubp/volltexte/2013/6700/.
Повний текст джерелаFür die ökologische Risikobewertung von Chemikalien sind individuenbasierte Populationsmodelle ein vielversprechendes Werkzeug um heutige Bewertungen ökologisch realistischer zu gestalten. Allerdings ist die Entwicklung und Parametrisierung derartiger Modelle zeitaufwendig und oft wenig systematisch. Standardisierte, geprüfte Untermodelle, die Einzelorganismen beschreiben, würden die individuenbasierte Modellierung effizienter und kohärenter machen. In meiner Dissertation habe ich daher untersucht, inwieweit sich die Dynamic Energy Budget-Theorie (DEB) als Standardmodell innerhalb individuenbasierter Populationsmodelle eignet, und zwar sowohl für die ökologische Risikobewertung als auch für die theoretische Populationsökologie. Zunächst habe ich eine generische Implementierung der DEB-Theorie im Rahmen individuenbasierter Modellen (IBM) erstellt: DEB-IBM. Dieses Werkzeug nutzend habe ich dann untersucht, ob es mit Hilfe der DEB-Theorie gelingt, ausgehend von den Eigenschaften und Aktivitäten einzelner Individuen, Populationsdynamik vorherzusagen. Wir nutzten dabei Daphnia magna als Modellart, für die Daten auf der Individuenebene verfügbar waren, um das Modell zu parametrisieren, sowie Populationsdaten, mit denen Modellvorhersagen verglichen werden konnten. DEB-Theorie war in der Lage, beobachtete Populationswachstumsraten sowie die maximalen Abundanzen korrekt vorherzusagen, und zwar für verschiedene Umweltbedingungen. Für Phasen des Rückgangs der Population allerdings, wenn die für die Daphnien verfügbare Nahrungsmenge gering war, kam es zu Abweichungen. Es waren deshalb zusätzliche Annahmen über nahrungsabhängige Sterblichkeit von juvenilen Daphnien erforderlich, um die gesamte Populationsdynamik korrekt vorherzusagen. Das resultierende Modell konnte dann, ohne weitere Kalibrierungen, den für Daphnien charakteristischen Wechsel zwischen Populationszyklen mit großen und kleinen Amplituden richtig vorhersagen. Wir folgern daraus, daß Ebenen übergreifende Tests dabei helfen, Lücken in aktuellen Theorien über Einzelorganismen aufzudecken Dies trägt zur Theorieentwicklung bei und liefert Grundlagen für individuenbasierte Modellierung und Ökologie. Über diese Grundlagenfragen hinaus haben wir überprüft, ob DEB-Theorie in Kombination mit IBMs es ermöglicht, den Effekt von chemischem Streß auf Individuen auf die Populationsebene zu extrapolieren. Wir nutzten Daten über die Auswirkungen von 3,4 Dichloroanalin auf einzelne Daphnien, die zeigten daß im Wesentlichen die Reproduktion, nicht aber das Wachstum beeinträchtigt ist. Mit entsprechenden Annahmen konnte unser Modell den Effekt auf Populationsebene, für den unabhängige Daten vorlagen, korrekt vorhersagen. DEB-Theorie in Kombination mit individuenbasierter Modellierung birgt somit großes Potential für einen standardisierten modellbasierten Ansatz in der ökologischen Risikobewertung von Chemikalien.
Martineau, Eugénie. "Linking single cell directionality to dynamic multicellular transitions in Myxococcus xanthus : a multiscale analysis." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0089.
Повний текст джерелаThe δ-proteobacteria Myxococcus xanthus has been a model of study for decades for its self-organized behavior as a response of environmental stimuli. It colonizes favorable ecological niches by using surface motility. In particular, this motility allows M.xanthus to predate collectively over prey microorganisms, while under starvation they start a developmental process to form macroscopic fruiting bodies, filled with environmental resistant myxospores. All these multicellular behaviors require a dynamic control of the cell polarity established by the polarity proteins MglA, MglB and RomR. Together, they define the direction of movement of the cell, which can be rapidly inverted by the Frz chemosensory system (reversion). In this thesis work, through combined computational/experimental approaches, we highlight that the regulation system forms a new type of biochemical oscillator, controlled by two proteins RomR and FrzX, which act together through complementary action to trigger the reversion at the lagging pole. The unique architecture of this system allows a wide response to various stimuli, which could be very beneficial for collective cell behaviors. To understand the importance of these transitions, we have developed a new high-resolution single cell assay linking single cMARTINEAU EUGENIE 2018AIXM0089/016ED62 2018/03/21 62 SCES SCHell behaviors to multicellular structures at unprecedented spatial and temporal resolutions. This way, we have investigated the role of the newly identified biochemical oscillator in the multicellular model of predation
Ilies, Remus. "A dynamic multilevel model of task motivation linking personality, affective reactions to feedback and self-regulation." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0000921.
Повний текст джерелаMartin, Benjamin [Verfasser], and Volker [Akademischer Betreuer] Grimm. "Linking individual-based models and dynamic energy budget theory : lessons for ecology and ecotoxicology / Benjamin Martin. Betreuer: Volker Grimm." Potsdam : Universitätsbibliothek der Universität Potsdam, 2013. http://d-nb.info/1038853052/34.
Повний текст джерелаCampos-Candela, Andrea. "Linking individual behaviour and life history: bioenergetic mechanisms, eco-evolutionary outcomes and management implications." Doctoral thesis, Universidad de Alicante, 2019. http://hdl.handle.net/10045/89047.
Повний текст джерелаMukherjee, Joy. "A Runtime Framework for Parallel Programs." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/28756.
Повний текст джерелаPh. D.
Shen, Lirui, Songcheng Xu, Kun Wu, and Guoying Li. "Novel method for preparing fish collagen gels with excellent physicochemical properties via the dehydration of ethanol - 122." Verein für Gerberei-Chemie und -Technik e. V, 2019. https://slub.qucosa.de/id/qucosa%3A34167.
Повний текст джерелаSjöberg, Ylva. "Linking water and permafrost dynamics." Doctoral thesis, Stockholms universitet, Institutionen för naturgeografi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-116647.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript.
Li, Chengkai. "Computational design of polymer nanocomposites." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/230364/1/Chengkai_Li_Thesis.pdf.
Повний текст джерелаDeacon, Oliver Mario. "Structural dynamics and spectroscopic studies of human cytochrome c : linking dynamics and disease." Thesis, University of Essex, 2018. http://repository.essex.ac.uk/22285/.
Повний текст джерелаTidd, Alexander. "Spoilt for choice? : linking individual fishing behaviour with fleet dynamics." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/12828.
Повний текст джерелаDahlgren, Johan Petter. "Linking plant population dynamics to the local environment and forest succession." Doctoral thesis, Stockholms universitet, Botaniska institutionen, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-8291.
Повний текст джерелаDiBari, John Nicholas. "Linking patch dynamics, landscape organization, patch-size scaling, and landscape connectivity." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/280167.
Повний текст джерелаGamal, El Dien Hamed Mohammed Abdel Ghany Mansour. "Geochemical Records Linking Plate Tectonics with Mantle Dynamics: Neoproterozoic and Beyond." Thesis, Curtin University, 2020. http://hdl.handle.net/20.500.11937/82663.
Повний текст джерелаGauthier-Fauteux, Simon. "Linking fluvial dynamics to white sturgeon habitat in the Nechako River, BC." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/60693.
Повний текст джерелаArts, Faculty of
Geography, Department of
Graduate
Hamilton, R. B. "From horse to herd : linking within-host and population level epidemic dynamics." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603617.
Повний текст джерелаSadia, Rina. "A System Dynamics Approach Linking Employee Health, Quality Culture and Organizational Effectiveness." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/26349.
Повний текст джерелаPh. D.
Arab, B., A. Shokuhfar, and S. Ebrahimi-Nejad. "Glass Transition Temperature of Cross-Linked Epoxy Polymers: a Molecular Dynamics Study." Thesis, Sumy State University, 2012. http://essuir.sumdu.edu.ua/handle/123456789/35102.
Повний текст джерелаBodmer, Pascal [Verfasser]. "Linking Carbon Dynamics in Stream Ecosystems to Dissolved Organic Matter Quality / Pascal Bodmer." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1097263495/34.
Повний текст джерелаLewis, Timothy D. "Linking soil erosion processes with arable weed seedbank dynamics to inform sustainable cropping." Thesis, University of Dundee, 2014. https://discovery.dundee.ac.uk/en/studentTheses/48280cfd-6ec8-480a-bba5-3a98bea5b605.
Повний текст джерелаRegier, Peter. "Linking Organic Matter Dynamics to Management, Restoration, and Climate in the Florida Everglades." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3385.
Повний текст джерелаLee, Brenda Ching Tsia. "The linking of process modeling of hopper-bottomed clarifier and computational fluid dynamics." Thesis, Swansea University, 2009. https://cronfa.swan.ac.uk/Record/cronfa42841.
Повний текст джерелаRiera, Domínguez María Graciela. "Linking cell dynamics with coexpression networks to characterize key events in chronic virus infection." Doctoral thesis, Universitat Pompeu Fabra, 2018. http://hdl.handle.net/10803/665626.
Повний текст джерелаLa respuesta inmune del huésped contra una infección requiere la acción coordinada de varios tipos de células que, de forma dinámica, se adapta a la amenaza del patógeno. Debido a la complejidad de dicha respuesta, la mayoría de los estudios inmunológicos se han centrado en pocos genes, proteínas o en células específicas. Con el desarrollo de tecnologías "ómicas" y métodos de análisis computacionales, los intentos de analizar y comprender sistemas complejos ahora son más factibles. Sin embargo, la descomposición de datos de transcriptomas, generados a partir de órganos completos sigue siendo un desafío importante. Aquí he combinado Weighted Gene Coexpression Network Analysis (WGCNA) y Digital Cell Quantifier (DCQ) para analizar los perfiles transcriptómicos de bazos de ratón, a diferentes tiempos, durante la infección aguda y crónica por el virus de la Coriomeningitis Linfocítica Aguda (LCMV). Este método nos ha permitido generar hipótesis sobre funcionamiento del complejo sistema inmune después de una perturbación inducida por el virus. Esta estrategia fue validada al predecir con éxito varios fenómenos inmunes conocidos, como la expansión de los linfocitos T citotóxicos (CTL) y el fenómeno conocido como agotamiento. Además, he predicho y posteriormente verificado de forma experimental que las células efectoras T CD8+ específicas de virus, con un perfil de transcriptoma efector temprano, participan en la adaptación a la amenaza del virus. Por lo tanto, la conexión entre la expresión de genes y la cinética de las células del sistema inmune, proporciona nuevos conocimientos sobre los complejos procesos inmunes en tejidos infectados.
Paul, Andrew J. "Recruitment dynamics in bull trout (Salvelinus confluentus), linking theory and data to species management." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0015/NQ54802.pdf.
Повний текст джерелаHerman, John E. "Linking Microbial Community Dynamics to Litter and Soil Chemistry: Understanding the Mechanisms of Decomposition." University of Toledo / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1278500390.
Повний текст джерелаMenden-Deuer, Susanne. "Linking individual foraging strategies with ecological dynamics : quantifying zooplankton movements in heterogeneous resource distributions /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/11012.
Повний текст джерелаWarren, Derek. "The role of Sla1p in linking actin dynamics to the endocytic machinery in Saccharomyces cerevisiae." Thesis, University of Glasgow, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394828.
Повний текст джерелаEvans, Derek. "LINKING CRITICAL SOURCE AREAS OF PHOSPHORUS TO STORMFLOW DYNAMICS IN THREE CENTRAL ILLINOIS AGRICULTURAL WATERSHEDS." OpenSIUC, 2013. https://opensiuc.lib.siu.edu/theses/1231.
Повний текст джерелаCabrera-García, Leonardo. "Linking social and ecological dynamics for bird conservation : protecting the endangered Sierra Madre sparrow in Chichinautzin, Mexico." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102793.
Повний текст джерелаCommunity-based biodiversity conservation efforts have been recently recognized as an important option for safeguarding ecosystems while reducing land use conflicts arising from the material, cultural and spiritual needs of local inhabitants. Community involvement is particularly critical for conservation in anthropogenic habitats. Few studies have linked the ecological impacts of community land use practices with the auto-ecological requirements of dependent bird species. In this study I examine the conservation possibilities for the endangered Sierra Madre sparrow (Xenospiza baileyi) by considering three basic elements: the ecological requirements of the bird, the effects of traditional land use practices in shaping the habitat, and the economic and social conditions that influence current and future land use decisions. I draw on ecological field studies, on traditional ecological knowledge systems, and studies of the political ecological context that influences local practices.
The studies were carried out from 2000-2003 and employed a combination of ethnographic, participative and spatial-ecological approaches to address human-land interactions and their impacts on the sparrow habitat. Social data were obtained through nine workshops which included site visits, transect walks, participatory mapping, oral histories and semi-structured interviews. Ecological data were obtained from landscape ecology analysis, vegetation post-disturbance assessments and detailed bird's nest-site selection analysis.
Results indicate that local people, principally herders, hold a rich knowledge of fire use to achieve diverse purposes, including pasture renewal, grassland maintenance and grass species selection, and prevention of dangerous fires. In order to accomplish their goals, herders have established rotational fire and grazing regimes that consider timing, frequency, location and extent of these disturbance-based practices. This rotational system was found to benefit the Sierra Madre sparrow by maintaining the grassland at the scales needed by the sparrow for nesting. Multiscale habitat recommendations for the species' conservation were derived from this socio-ecological interaction and dynamics.
Unfortunately, external conservation perspectives and interests and internal land tenure conflicts have altered this rotational regime and local perspectives on resource management that threaten the resilience of this social-ecological system. Consequently, traditional ecological knowledge on grassland management can be on risk of disappearing and, with it, important native grasses and grasslands are being made vulnerable. The survival of the Sierra Madre sparrow in particular and of associated biodiversity in general, is in peril if these conflicts are not solved in a relatively short time. A community-based fire co-management program is recommended to promote integrative bird conservation-local development scenarios.
Boano, Camillo. "Dynamics of linking reconstruction and development in housing and settlements for forced migrants in post-disaster situations." Thesis, Oxford Brookes University, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444285.
Повний текст джерелаTruelove, Gemma Jane. "An empirical study linking behaviour and population dynamics : altering spatial food availability in a mite model system." Thesis, University of Stirling, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442707.
Повний текст джерелаXu, Yi. "Modeling the Seasonal and Interannual Variability of Peruvian Anchovy (Engraulis ringens) Population Dynamics: Linking Environmental Conditions with Fish." Fogler Library, University of Maine, 2009. http://www.library.umaine.edu/theses/pdf/XuY2009.pdf.
Повний текст джерелаMünkemüller, Tamara. "Density regulation: linking theory and applications to understand population - to community -level dynamics and responses to environmental change /." Leipzig : UFZ, 2008. http://www.ufz.de/data/ufzdiss_09_20089156.pdf.
Повний текст джерелаZöllner, Robert [Verfasser], Berenike [Gutachter] Maier, and Tobias [Gutachter] Bollenbach. "Linking intercellular forces to colony dynamics and fitness in bacterial populations / Robert Zöllner ; Gutachter: Berenike Maier, Tobias Bollenbach." Köln : Universitäts- und Stadtbibliothek Köln, 2019. http://d-nb.info/1182533299/34.
Повний текст джерелаpagani, giulia [Verfasser], Holger [Gutachter] Gohlke та Birgit [Gutachter] Strodel. "Linking structure and dynamics of integrin αIIbβ3 to its biological function via molecular dynamics simulations and free energy calculations / giulia pagani ; Gutachter: Holger Gohlke, Birgit Strodel". Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2018. http://d-nb.info/1166950522/34.
Повний текст джерелаPagani, Giulia [Verfasser], Holger [Gutachter] Gohlke та Birgit [Gutachter] Strodel. "Linking structure and dynamics of integrin αIIbβ3 to its biological function via molecular dynamics simulations and free energy calculations / giulia pagani ; Gutachter: Holger Gohlke, Birgit Strodel". Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2018. http://d-nb.info/1166950522/34.
Повний текст джерелаPanter, Paige Elizabeth. "Exploring the role of cell-wall pectin cross-linking in freezing tolerance and guard cell dynamics in Arabidopsis thaliana." Thesis, Durham University, 2019. http://etheses.dur.ac.uk/12957/.
Повний текст джерелаDannberg, Juliane [Verfasser], Stephan Vladimir Akademischer Betreuer] Sobolev, Michael [Akademischer Betreuer] [Weber, and Volker [Akademischer Betreuer] John. "Dynamics of mantle plumes : linking scales and coupling physics / Juliane Dannberg ; Stephan Vladimir Sobolev, Michael H. Weber, Volker John." Potsdam : Universität Potsdam, 2016. http://d-nb.info/1218400463/34.
Повний текст джерелаDannberg, Juliane [Verfasser], Stephan Vladimir [Akademischer Betreuer] Sobolev, Michael H. [Akademischer Betreuer] Weber, and Volker [Akademischer Betreuer] John. "Dynamics of mantle plumes : linking scales and coupling physics / Juliane Dannberg ; Stephan Vladimir Sobolev, Michael H. Weber, Volker John." Potsdam : Universität Potsdam, 2016. http://d-nb.info/1218400463/34.
Повний текст джерелаOsei, Tutu Anthony [Verfasser], Michael [Akademischer Betreuer] Weber, Bernhard [Akademischer Betreuer] Steinberger, and Irina [Akademischer Betreuer] Rogozhina. "Linking global mantle dynamics with lithosphere dynamics using the geoid, plate velocities and lithosphere stress state as constraints : lithosphere and mantle dynamics coupling / Anthony Osei Tutu ; Michael H. Weber, Bernhard Steinberger, Irina Rogozhina." Potsdam : Universität Potsdam, 2018. http://d-nb.info/1218403330/34.
Повний текст джерелаOsei, Tutu Anthony [Verfasser], Michael H. [Akademischer Betreuer] Weber, Bernhard [Akademischer Betreuer] Steinberger, and Irina [Akademischer Betreuer] Rogozhina. "Linking global mantle dynamics with lithosphere dynamics using the geoid, plate velocities and lithosphere stress state as constraints : lithosphere and mantle dynamics coupling / Anthony Osei Tutu ; Michael H. Weber, Bernhard Steinberger, Irina Rogozhina." Potsdam : Universität Potsdam, 2018. http://d-nb.info/1218403330/34.
Повний текст джерелаRonget, Victor. "The relationships linking age and body mass to vital rates : a comparative perspective in birds and mammals." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1304/document.
Повний текст джерелаIndividuals varies in terms of survival and reproduction. Most of those variations in vital rates can be linked to individual characteristics such as age or body mass. Demographic models were developed to make prediction on those trait-structured populations and are now often used to manage wild populations. However, the amount of data needed to perform those models is not available for every populations. To overcome this issue, I tried in my thesis to assess the general patterns for the relationships linking age and body mass to the vital rates in birds and mammals. By comparing relationship extracted in the literature, I was then able to assess the general effect of body or age on vital rates as well as the biological factor explaining the variation of those relationships between species and populations. I first assess how body mass influence vital rates in birds and mammals. I demonstrated the positive effect of offspring body mass on offspring survival and showed how the relative importance of each causes of mortality influence this relationship, with for instance a negative effect of the predation rate on the intensity of the relationship. I also showed that mother body mass is positively related to offspring body mass and that heavier mother are also more likely to reproduce. On a second part I focused on describing the relationship between age and survival for mammals. We built a database MALDDABA compiling all relationships linking vital rates to age for wild mammals from life tables reported in the literature. Using life table data compiled in the database I was able to demonstrate that females live on average longer than males in wild populations of mammals. I then critically assess the metrics of longevity and provide new insight to describe the relationship between mortality and age. With my thesis I provided new views on the uses of comparative approach to highlight the major factors influencing the population dynamic in the wild
Motleleng, Liabo. "Linking microbial community dynamics and performance of a biological sulphate reducing system using a mixed volatile fatty acid stream as electron donor." Doctoral thesis, Faculty of Engineering and the Built Environment, 2021. http://hdl.handle.net/11427/33883.
Повний текст джерелаRinke, Karsten. "Species-oriented model approaches to Daphnia spp.: linking the individual level to the population level." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2006. http://nbn-resolving.de/urn:nbn:de:swb:14-1146736164110-76933.
Повний текст джерелаThe scope of this thesis was to develop a comprehensive model system of the genus Daphnia, a key organism in the pelagic food web of lakes and reservoirs and a widely used model organism in experimental and theoretical ecology. Although its central role in applied and basic research in aquatic ecology is obvious, there are still fundamental problems in modelling the observed dynamics of Daphnia (for details see chapter 2). Therefore, a basic motivation of this work was to use scientific results obtained in independently conducted research for developing a model that brings these results into context. Instead of following a "problem-oriented" paradigm applicable to a single, well defined problem or scientific hypothesis, the underlying concept of the emerging model system was considered to be "species-oriented". Thus, various relevant processes are included into the framework in order to simulate the dynamics of daphnids displayed on different levels of biological organization. To facilitate its application to various problems in ecological research on the genus Daphnia, the model system fulfills the following three important properties: (1) model outputs are thoroughly validated on experimental data in order to guarantee sound quantitative outputs of the model system (2) the system spans over different levels of biological organization with special emphasis laid upon the individual level and the population level (3) the model´s architecture follows a nested design with a defined individual level model that is integrated into a population level model The whole model system is able to describe an individual´s development over time on basis of physiological properties of the organism and, furthermore, how these individual level processes interact with the dynamics on the population level. Due to its nested design, applications of separate submodels (e.g. the individual-level model) are possible
Figueiredo, Alana dos Reis. "Estudo teorico e experimental de proteomica estrutural por espectrometria de massas acoplada a ligação cruzada." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250213.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica
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Resumo: A espectrometria de massas (MS) desempenha um papel fundamental na proteômica, pois permite a identificação de proteínas, seqüenciamento de peptídeos, determinação de modificações pós-traducionais e análise quantitativa de expressão. Além das análises envolvendo a estrutura primária, há um grande interesse em se aplicar MS para análise de estruturas superiores (terciárias e quaternárias) de proteínas e uma das abordagens promissoras nesta área é a MS acoplada à ligação cruzada. Nesta abordagem, um reagente bifuncional liga covalentemente resíduos de aminoácidos espacialmente próximos e a distância máxima entre esses resíduos é dado pelo tamanho do agente de ligação cruzada (ALC). Nesse trabalho avaliou-se a extensão e exatidão das distâncias interresíduos tanto por simulações de dinâmica molecular quanto por experimentos de MS. As faixas de distâncias calculadas mostram que há sempre uma distância mínima entre resíduos ao qual o ALC pode se ligar, além de determinar o valor máximo para cada um dos ALC estudados (DSG, DSS e DSSeb). Os dados experimentais com proteínas modelo (Ubiquitina, Citocromo C e Mioglobina) mostraram ainda que todos dos peptídeos que apresentavam ligações cruzadas estavam dentro da faixa de alcance determinadas pelas simulações de dinâmica molecular, confirmando a exatidão do método. Os novos valores de tamanho das moléculas de ALC estudados podem agora ser utilizados para a determinação de estruturas superiores de proteínas através da técnica de MS acoplada a ligação cruzada
Abstract: Mass spectrometry (MS) plays a key role in proteomics because it allows the identification of proteins, peptide sequencing, determination of post-translational modifications and quantitative expression analysis. There is a great interest in using MS to perform analysis beyond the primary structure, i. e. tertiary and quaternary structures, and one of the most promising approaches in this filed is MS coupled to cross-linking technique. In this approach, a bifunctional reagent covalently binds amino acid residues that are close in space and the maximum distance between these residues is given by the arm length of the cross-linking agent. In this study we evaluated the extent and accuracy of the inter-residues distances by both molecular dynamics simulations and MS experiments. Simulated distance ranges showed that there is a minimum distance between residues to which the reagent can bind and a maximum value for each of the studied reagents (DSG, DSS and DSSeb). The experimental data from model proteins (Ubiquitin, Cytochrome C and Myoglobin) also showed that all the detected modified peptides were within the ranges determined by the molecular dynamics simulations, confirming the accuracy of the method. The new space arm length values of the reagent molecules can now be used for the determination of proteins higher structures by the MS coupled to cross-linking technique
Mestrado
Quimica Organica
Mestre em Química