Дисертації з теми "DSB substrates"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "DSB substrates".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Casale, Marco. "Réalisation d'un laser DFB hybride sur substrat de verre." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENT011/document.
Повний текст джерелаThe current needs of optical telecommunications and sensors require developing integrated optical circuits providing different and heterogeneous functions on the same substrate. The main issue is the incompatibility between the manufacturing processes of these optical functions. This work deals with the integration of active (emission, amplification) and passive (multiplexing, filtering, etc.) functions on a passive glass substrate. Its aim is to develop a DFB three-dimensional hybrid laser by ion-exchange in passive glass. This laser is made of a Bragg channel waveguide, selectively buried in the passive glass substrate, loaded by a plane waveguide, defined at the surface of an Er3+:Yb3+ codoped active glass wafer. It emits a (420±15) µW laser signal at 1534 nm for (390±20) mW injected pump power. Hence this device opens the way towards the integration of active functions, located at the surface of the passive glass substrate, with passive ones, spreading at its surface and in its volume
Almeida, Fernando Luis de. "Desenvolvimento de um sensor eletroquímico planar modificado com 1-2 Diaminobenzeno (DAB) para monitoração de nitrito por FIA-automatizada." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/3/3140/tde-30092010-105650/.
Повний текст джерелаIn this Master of Science, it has been developed an electrochemical planar sensor defined with three electrodes for nitrite measuring and its interferents (uric acid, ascorbic acid and paracetamol). In the experimental procedure, it is shown the development of an extracorporis set up (mini-pump, solenoid valves and analysis cell) which is coupled to a system of Flow-injection Analysis (FIA). This work also describes the sensor fabrication using alumina substrates, the standard cleaning of the electrodes, the process to obtain Ag/AgCl reference electrodes in HCl (0.1 mol L-1) and electrochemical activation of the working-electrode surface with H2SO4 (0.1 mol L-1). Following, it is shown the study of drying for electrodes at room temperature with exposition to room light and their characterization using three different methods: i) reversibility test (redox current vs. potential); ii) variation of the potential with the time (thermodynamics degradation coefficient) and iii) variation of the potential with the chloride concentration. Also, it is presented the characterization of the effective exposed area of the working electrode and the results of the deposited 1-2 Diaminobenzene (DAB) is presented as a selective polymer. In addition, the results and discussions of the pseudo-reference (recovered or not with Nafion® 117) are presented. Related to irreversibility is the hysteresis associated to the reference electrodes which resulted in the order of 40 mVAg/AgCl Nafion® 117. The observed thermodynamic potentials were of (100 ± 6) mVAg/AgCl Nafion® 117 for pseudo-electrodes recovered with Nafion® 117. Later on, a careful study of the response to nitrite and to its interferents was performed by means of the DPV technique (Differential Pulse Voltammetry). In order to avoid accelerated degradation (corrosion) of the polymer, measurements were performed at a potential of 0,5 VAg/AgCl Nafion® 117. In this condition, nitrite was monitored using the automatized-FIA (Flow Injection Analysis) for concentration in the range of 50 to 250 mol L-1. The results have shown that the planar amperometric sensor for nitrite measuring is usefull since it was observed excellent performance related to reproducibility (99.66%), sensitivity (90 microA mol-1 L mm-2), selectivity (0.32%), repetibility (91.28%) and stability (15.0 ± 0.3 pA).
Li, Hongbo. "FEM analysis with DSC modeling for materials in chip-substrate systems." Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/280363.
Повний текст джерелаGao, Zhaoli. "CNT-TIM fabrication on aluminium alloy substrates /." View abstract or full-text, 2010. http://library.ust.hk/cgi/db/thesis.pl?MECH%202010%20GAO.
Повний текст джерелаAu, Ping Tong. "Mechanically stabilized bistable FLCD on flexible substrates /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?ECED%202006%20AU.
Повний текст джерелаPoller, Tilo. "Thermal and thermal-mechanical simulation for the prediction of fatigue processes in packages for power semiconductor devices." Doctoral thesis, Universitätsbibliothek Chemnitz, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-154320.
Повний текст джерелаFür die Entwicklung von Umrichtern ist die Kenntnis über die Zuverlässigkeit der Leistungselektronik ein wichtiges Kernthema. Insbesondere für Offshore-Anwendungen ist das Wissen über die stattfindenden Ermüdungsprozesse und die Abschätzung der zu erwartenden Lebensdauer der Bauteile essentiell. Hierfür hat sich die Simulation als ein wichtiges Werkzeug für die Entwicklung und Lebensdauerbewertung von leistungselektronischen Anlagen etabliert. In der folgenden Arbeit wird das thermische und das thermisch-mechanische Verhalten der Leistungselektronik mittels Simulationen untersucht. Hierzu wird ein Vergleich zwischen verschiedenen thermischen Modellen für Leistungsbauelemente durchgeführt. Schwerpunkt ist die Beschreibung der thermischen Kopplung zwischen den Chips und deren Einfluss auf die Lebensdauerabschätzung. Ein weiterer Schwerpunkt ist das Leistungsmodul, welches sich als ein Standardgehäuse etabliert hat. Dazu wird erklärt, wie die Variation der Einschaltzeit im aktiven Lastwechseltest den Fehlermodus dieses Gehäusetyps beeinflusst. Weiterhin wird untersucht, wie SiC als Leistungshalbleiter und DAB als Substrat die Zuverlässigkeit beein- flusst. Der Press-Pack ist für Hochleistungsapplikationen von hohem Interesse, da dieses Gehäuse im elektrischen Fehlerfall ohne äußere Unterstützung kurzschliesst. Jedoch ist das Wissen über diese Gehäusetechnologie unter aktiven Lastwechselbedingungen sehr limitiert. Mit Hilfe von Simulationen wird dieses Verhalten untersucht und mögliche Schwachpunkte abgeleitet. Am Ende der Arbeit werden Möglichkeiten untersucht, wie Mithilfe von FEM Simulationen die Lebensdauer von Leistungsmodulen evaluiert werden kann
Ge, Xiang. "Electrochemical deposition of fluoridated calcium phosphate on titanium substrates /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?MECH%202008%20GE.
Повний текст джерелаLiu, Chun Kit. "Process development and characterization of inductors for organic substrates /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?MECH%202003%20LIU.
Повний текст джерелаIncludes bibliographical references (leaves 70-72). Also available in electronic version. Access restricted to campus users.
Zhang, Baoshe. "A study of substrate--liquid crystal interaction /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202003%20ZHANG.
Повний текст джерелаIncludes bibliographical references (leaves 176-186). Also available in electronic version. Access restricted to campus users.
Poller, Tilo. "Thermal and thermal-mechanical simulation for the prediction of fatigue processes in packages for power semiconductor devices." Doctoral thesis, Universitätsverlag der Technischen Universität Chemnitz, 2014. https://monarch.qucosa.de/id/qucosa%3A20135.
Повний текст джерелаFür die Entwicklung von Umrichtern ist die Kenntnis über die Zuverlässigkeit der Leistungselektronik ein wichtiges Kernthema. Insbesondere für Offshore-Anwendungen ist das Wissen über die stattfindenden Ermüdungsprozesse und die Abschätzung der zu erwartenden Lebensdauer der Bauteile essentiell. Hierfür hat sich die Simulation als ein wichtiges Werkzeug für die Entwicklung und Lebensdauerbewertung von leistungselektronischen Anlagen etabliert. In der folgenden Arbeit wird das thermische und das thermisch-mechanische Verhalten der Leistungselektronik mittels Simulationen untersucht. Hierzu wird ein Vergleich zwischen verschiedenen thermischen Modellen für Leistungsbauelemente durchgeführt. Schwerpunkt ist die Beschreibung der thermischen Kopplung zwischen den Chips und deren Einfluss auf die Lebensdauerabschätzung. Ein weiterer Schwerpunkt ist das Leistungsmodul, welches sich als ein Standardgehäuse etabliert hat. Dazu wird erklärt, wie die Variation der Einschaltzeit im aktiven Lastwechseltest den Fehlermodus dieses Gehäusetyps beeinflusst. Weiterhin wird untersucht, wie SiC als Leistungshalbleiter und DAB als Substrat die Zuverlässigkeit beein- flusst. Der Press-Pack ist für Hochleistungsapplikationen von hohem Interesse, da dieses Gehäuse im elektrischen Fehlerfall ohne äußere Unterstützung kurzschliesst. Jedoch ist das Wissen über diese Gehäusetechnologie unter aktiven Lastwechselbedingungen sehr limitiert. Mit Hilfe von Simulationen wird dieses Verhalten untersucht und mögliche Schwachpunkte abgeleitet. Am Ende der Arbeit werden Möglichkeiten untersucht, wie Mithilfe von FEM Simulationen die Lebensdauer von Leistungsmodulen evaluiert werden kann.
Pietranico, Sylvain. "Analyse de matériaux pour la modélisation des mécanismes de défaillance dans les modules électroniques de puissance." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2010. http://tel.archives-ouvertes.fr/tel-00543040.
Повний текст джерелаFeng, Zhihong. "Enhanced device performance of III-nitride HEMTs on sapphire substrates by MOCVD /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?ELEC%202006%20FENG.
Повний текст джерелаCheng, Pak Lun. "Experiments and quantitative resistance analyses of as-deposited resistors on organic substrates /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?MECH%202003%20CHENG.
Повний текст джерелаGaimard, Quentin. "Diodes lasers DFB à couplage par l'indice émettant entre 2 µm et 3,3 µm sur substrat GaSb." Thesis, Montpellier 2, 2014. http://www.theses.fr/2014MON20079/document.
Повний текст джерелаDevelopment of a reliable, real-time, selective, sensitive and suitable technique for atmospheric trace gas spectroscopy is a critical challenge in science and engineering, for sanitary, ecological and industrial issues. Tunable single-frequency lasers in the 2µm to 3.3µm wavelength range, working in continuous regime at room temperature, can be used in absorption spectroscopy to identify and quantify several atmospheric gases. We report here on the design, the technological development and the performances of 1st and 2nd order index-coupled distributed-feedback (DFB) antimonide-lasers diodes in the 2µm to 3.3µm wavelength range. The first part of this document establishes the context of the thesis, introduces the DFB theory and our modelisation. The second part presents the technological fabrication of the two different components: the side wall corrugated DFB lasers and the buried DFB lasers. The third part shows the performances of the components and the first tests on gas measurement.This work has led to the development of two different kinds of single-frequency laser diodes with high optical power and spectral purity. The fabricated prototypes will soon be used on gas spectroscopy set-up
Lee, Fuk Kay. "Control of alignment for liquid crystal on inhomogeneous substrate /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202005%20LEE.
Повний текст джерелаTung, Wang Kei. "FRP debonding from concrete substrate : theoretical and experimental approach /." View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?CIVL%202002%20TUNG.
Повний текст джерелаIncludes bibliographical references (leaves 109-110). Also available in electronic version. Access restricted to campus users.
Matta, Elie. "Characterization of DNA ADP-Ribosylation Mechanism and its Role in DNA Damage Signaling Insight into DNA Substrate Specificity of PARP1-Catalysed DNA Poly(ADP-Ribosyl)ation Role of PARP-catalyzed ADP-ribosylation in the Crosstalk Between DNA Strand Breaks and Epigenetic Regulation." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASS058.
Повний текст джерелаDNA-dependent poly(ADP-ribose) polymerases (PARPs) PARP1, PARP2 and PARP3 act as DNA break sensors signaling DNA damage. Upon detecting DNA damage, these PARPs use nicotine adenine dinucleotide as a substrate to synthesize a monomer or polymer of ADP-ribose (MAR or PAR, respectively) covalently attached to the acceptor residue of target proteins. Recently, it was demonstrated that PARP1–3 proteins can directly ADP-ribosylate DNA breaks by attaching MAR and PAR moieties to terminal phosphates. Nevertheless, little is still known about the mechanisms governing substrate recognition and specificity of PARP1, which accounts for most of cellular PARylation activity, as well, about proteins responsible for detection and removal of ADP-ribosylated DNA adducts and its role in multitude of cellular processes.In this study we provide a detailed characterization of PARP1 DNA substrate specificity and mechanisms of DNA PARylation. We showed that the 3′-terminal phosphate residue at double-strand DNA break ends served as a major acceptor site for PARP1-catalysed PARylation depending on the orientation and distance between DNA strand breaks in a single DNA molecule. Moreover, a preference for ADP-ribosylation of DNA molecules containing 3′-terminal phosphate over PARP1 auto-ADP-ribosylation was observed, and a model of DNA modification by PARP1 was proposed. Similar results were obtained with purified recombinant PARP1 and HeLa cell-free extracts. Thus, the biological effects of PARP-mediated ADP-ribosylation may strongly depend on the configuration of complex DNA strand breaks. Furthermore, we elaborated a new research technique to identify and validate proteins responsible for ADP-ribose-DNA adducts detection (“readers”) or removal (“erasers”). Our proteomic data revealed that MARylated DNA adducts selectively modulated DNA recognition of a large number of proteins involved in different cellular pathways. About 90 proteins including protein complexes were selected as potential MAR-DNA adduct readers. The role of DNA ADP-ribosylation in non-homologous end-joining (NHEJ) was partially characterized in an in vitro study. We demonstrated that ADP-ribosylation of DSB terminus can lead to inhibition of blunt DSB repair by canonical NHEJ if not removed by PARG glycohydrolase. Contrary, presence of a proximal nick with a stabilized apurinic/apyrimidinic site leads to increased NHEJ efficiency, apparently in ADP-ribosylation-independent manner. Finally we searched for novel PARP1, PARP2 and PARP3 inhibitors among derivatives of 1,4-dihydropyridine with DNA binding capacity. Our results revealed that some of NAD+ analogues analogs could be used by PARPs for DNA modification leading to stabilization of corresponding MARylated and PARylated adducts due to their PARG hydrolysis activity resistance. Taking together, these data highlight the physiological relevance and possible biological outcomes of PARP-catalyzed DNA-ADP-ribosylation such as providing a stable benchmark of the location of a DNA strand break on a chromatin map, recruitement of DNA repair proteins and inhibition of the toxic NHEJ
Nguyen, Duy Verfasser], and Volkhard [Akademischer Betreuer] [Helms. "Computational analysis of membrane transporters and their substrates / Duy Nguyen ; Betreuer: Volkhard Helms." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:291-scidok-ds-270436.
Повний текст джерелаHong, Fangjun. "Droplet spreading, substrate remelting and variable thermal contact resistance in microcasting /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?MECH%202005%20HONG.
Повний текст джерелаNg, Kung Yau. "ANKRA2 interacts with p35 and is a substrate for Cdk5/p35 /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20NG.
Повний текст джерелаShen, Chao. "Study of CMOS active pixel image sensor on SOI/SOS substrate /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?ELEC%202003%20SHEN.
Повний текст джерелаIncludes bibliographical references (leaves 67-69). Also available in electronic version. Access restricted to campus users.
Keung, Chi Wing. "Matrix-addressable III-nitride light emitting diode arrays on silicon substrates by flip-chip technology /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?ECE%202007%20KEUNG.
Повний текст джерелаChin, Wing Hong. "Identification of TrkB as a p35 interacting protein and a Cdk5 substrate /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20CHIN.
Повний текст джерелаLiang, Hu. "Fabrication of high power InGaN/GaN multiple quantum well blue LEDs grown on patterned Si substrates /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?ECED%202008%20LIANG.
Повний текст джерелаBarreau, Viktoriia Verfasser], and Eduard [Akademischer Betreuer] [Arzt. "Adhesion of micropatterned adhesives to rough substrates and at elevated temperatures / Viktoriia Barreau ; Betreuer: Eduard Arzt." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:291-scidok-ds-270351.
Повний текст джерелаSpengler, Christian [Verfasser]. "Exploring Microbial Adhesion Through Targeted Design of Bacterial Probes and Substrates in AFM-based Force Spectroscopy / Christian Spengler." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:291--ds-276530.
Повний текст джерелаWang, Yong. "Research on improvement of breakdown voltage of AlGaN/GaN HEMTs grown on Si(111) substrates by MOCVD /." View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?ECED%202009%20WANGY.
Повний текст джерелаXie, Yu-Tao. "Surface-enhanced hyper raman and surface-enhanced raman scattering : novel substrates, surface probing molecules and chemical applications /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?CHEM%202007%20XIE.
Повний текст джерелаLiu, Na. "Ordered ZnSe nanowire arrays grown on GaAs (111) substrate by molecular beam epitaxy /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202005%20LIU.
Повний текст джерелаJia, Shuo. "AlGaN/GaN high electron mobility transistors on silicon substrate for RF/microwave applications /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?ELEC%202005%20JIA.
Повний текст джерелаBendin, Eckhard. "Ostwalds biologische Energetik als Substrat der psychologischen Farbenlehre." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1200579355626-82806.
Повний текст джерелаWith today's understanding of colour perception as a self-regulating process of energy transformation and effects, biological psychology honours the contribution made by Wilhelm Ostwald over 100 years ago with his concept of ’biological energetics’. Energy considerations and interpretations determined also his ’psychological theory of colour’, the declared core of modern colour science. Even if critical reception has revealed also the limitations of this colour theory, Ostwald's energetic concept can certainly be seen as a pioneering vision, retaining its validity in many aspects in today's scientific knowledge base. In the context of close relationships between science and art, furthermore, attention must be drawn to fertile interactions, in particular in the influences of Ostwald’s ’energetics’ on the visual arts in the 20th century
Vandersand, Jr James Dennis. "GROWTH OF 6H-SIC HOMOEPITAXY ON SUBSTRATES OFF-CUT BETWEEN THE [01-10] AND [10-10] PLANES." MSSTATE, 2002. http://sun.library.msstate.edu/ETD-db/theses/available/etd-11072002-095154/.
Повний текст джерелаCheng, Kai. "Identification of Pctaire1 as a p35-interacting protein and a novel substrate for Cdk5 /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BICH%202003%20CHENG.
Повний текст джерелаIncludes bibliographical references (leaves 153-177). Also available in electronic version. Access restricted to campus users.
Pozzan, Andreza Mara 1989, Sidney Luiz 1971 Stürmer, and Universidade Regional de Blumenau Programa de Pós-Graduação em Engenharia Ambiental. "Produção de inóculo de fungos micorrízicos arbusculares utilizando resíduos sólidos como substrato." reponame:Biblioteca Digital de Teses e Dissertações FURB, 2015. http://www.bc.furb.br/docs/DS/2015/361418_1_1.pdf.
Повний текст джерелаCo-orientador: Luis Hamilton Pospissil Garbossa.
Dissertação (Mestrado em Engenharia Ambiental) - Programa de Pós-Graduação em Engenharia Ambiental, Centro de Ciências Tecnológicas, Universidade Regional de Blumenau, Blumenau.
Huang, Qunjian. "Surface-enhanced raman scattering and surface-enhanced hyper raman scattering : a systematic study of various probing molecules on novel substrates /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?CHEM%202003%20HUANG.
Повний текст джерелаPrandato, Emeline. "Relations structure-propriétés et résistance à l’endommagement de vernis acrylate photo-polymérisables pour substrats thermoplastiques : évaluation de monomères bio-sourcés et de nano-charges." Thesis, Lyon, INSA, 2013. http://www.theses.fr/2013ISAL0103/document.
Повний текст джерелаThe aim of this work was to develop 100% solids photo-polymerizable acrylate coatings, intended to protect thermoplastic pieces made of polycarbonate against mechanical damage, in particular scratches. The relationships between the composition, the structure and the properties of these coatings were examined. For this purpose the morphology, the thermomechanical properties and the scratch resistance of the materials, assessed by micro-scratch tests, were studied. The kinetics of the polymer network formation was also studied by photo-DSC experiments. All the materials feature a high elastic modulus and a broad mechanical relaxation in dynamic thermomechanical analysis. A 100% solids petro-based coating (standard) constituted the starting point of this work. First it was compared to a commercial photo-polymerizable coating containing solvents, specially designed to protect thermoplastic pieces. This commercial coating turned out to be more efficient against scratches. In a second time was studied the influence of the percentage of a multicyclic monomer, taking part in the composition of the standard petro-based coating, on the properties of the latter. The modification of its proportion does not bring any advantage concerning the scratch resistance. Silica, alumina and zirconia nanoparticles, dispersed in an acrylate monomer, were then incorporated in the standard petro-based coating. A particular organization of the silica or alumina nanoparticles in the materials could be observed by transmission electron microscopy. A high filler content is required to observe an increase in the elastic modulus and an enhancement of the scratch resistance of the coating (≥15% by weight for the nano-silica). Moreover, no change of the photo-polymerization kinetics was noticed through the addition of 5% by weight of nano-silica in the coating. Finally, some of the petro-based acrylate compounds of the standard coating were substituted by commercially available bio-based acrylate monomers. Both types of coatings feature similar polymerization kinetics. The conclusions concerning the comparison of the scratch resistance of the bio-based and standard petro-based coatings depend on their thickness. The incorporation of a bio-based monoacrylate compound in low thickness coatings tends to improve the elastic recovery. Isobornyl acrylate is particularly interesting since it also tends to delay the apparition of cracks along the scratch
Lau, Chi Ho. "Process development for the fabrication of suspended membrane with nanoscale through vias on a silicon substrate /." View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?MECH%202009%20LAU.
Повний текст джерелаRiedl, Thomas. "La0,7Sr0,3MnO3-Dünnschichten auf SrTiO3 (0 0 1)-Substrat: Struktur und Mn-Wertigkeit." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1211277073076-72652.
Повний текст джерелаRiedl, Thomas. "La0,7Sr0,3MnO3-Dünnschichten auf SrTiO3 (0 0 1)-Substrat: Struktur und Mn-Wertigkeit." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1211277073076-72652.
Повний текст джерелаAdenesky, Filho Eduardo, Rosete Pescador, and Universidade Regional de Blumenau Programa de Pós-Graduação em Engenharia Ambiental. "Resíduos agroindustrial da palmeira real da Austrália Archontophoenix alexandrae H. Wendl. & Drude, como componente para substratos de espécies ornamentais /." reponame:Biblioteca Digital de Teses e Dissertações FURB, 2007. http://www.bc.furb.br/docs/DS/2007/322263_1_1.pdf.
Повний текст джерелаSieczkowska, Barbara. "Functional polymer layers with protected amines." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1244668077080-12212.
Повний текст джерелаDiese Arbeit bezieht sich auf das Gebiet der Bionanotechnologie und betrifft ein neuartiges Verfahren zur selektiven Immobilisierung der DNA oder anderer Biomoleküle auf mikrostrukturierten Goldkontakten, welche dann ein koordiniertes Zusammenwirken von einzelnen Nanomolekülen ermöglichen, z.B. in einem Mikroreaktor. Die Immobilisierung solcher Nanoobjekte soll durch dünne Funktionsschichten realisiert werden, die die Anbindungsgruppen liefern. Folglich war das Hauptziel dieser Arbeit die Entwicklung von Polymermaterialien für dünne Funktionsschichten, die die Aufbringung einer großen Vielzahl von Funktionselementen oder metallischen Strukturen auf verschiedenen Substraten gestatten und die Strukturierung durch den Einsatz von lithographischen Methoden ermöglichen. Um dieses Konzept zu realisieren, war es notwendig, ein Polymersystem zu gestalten und zu entwickeln, welches auf geeignete photolabile Einheiten basiert und zusätzlich Ankergruppen hat, die mit spezifischen Substraten wie Gold verbunden ist. Dieses Terpolymerkonzept wurde gezielt aus drei Komponenten mit speziellen Funktionen in entsprechenden molaren Verhältnissen gebildet, die eine Abstimmung der Materialeigenschaften ermöglicht und folgendes bereitstellt: photolabile geschützte Aminogruppen für die photolitographische Strukturerzeugung mit freien Aminogruppen, welche für weitere Modifikationen verfügbar sind wie das Anhängen von Kolloiden, die Metallisierung oder Anfügung von DNA-Strängen; disulfide Derivate für die kovalente Anbindung auf der Goldoberfläche und Spacer-Gruppe für Verbesserung der Schichtenbildung. Diese multifunktionalen Terpolymere sollen durch eine freie radikalische Polymerisation von entsprechenden Monomeren synthetisiert werden. Obwohl diese Techniken erfolgreich sind, sind sie eingeschränkt durch ihre Komplexität, den strengen synthetischen Anforderungen, sowie der Inkompatibilität mit vielen funktionalen thermolabilen und hochreaktiven Funktionalitäten. Um diese Schwierigkeiten zu überwinden wurde eine Polymerisationstechnik für diese Arbeit genutzt, die auf der „lebenden“ freien radikalischen Polymerisation basiert. Eine hoch effiziente polymeranaloge Modifizierung erlaubt die Einführung von Funktionalitäten nach der Polymeraufbaureaktion. Die Herstellung von entsprechenden Präpolymeren Poly(Styrol-r-4-Propargyl-oxystyrol) wurde mittels einer kontrollierten Synthesemethodik „Nitroxid-mediated controled radical polymerisation“ (NMRP) durchgeführt, gefolgt von der Polymeranalogreaktion, die eine der effizientesten Click-Reaktion - die Cu(I) katalysierte 1,3-dipolar Cycloaddition von terminalen Alkinen an Aziden nach Huisgen nutzt, um weiter Funktionalitäten durch die Bildung eines stabilen 1,4-disubstituierten-[1,2,3]-Triazolringes anzufügen. Die Kombination von NMRP und Click-Chemie wurde zur Herstellung eines exakt definierten Random Copolymers genutzt. Es konnte bereits gezeigt werden, dass auch Blockcopolymere geschaffen werden können, die eine Möglichkeit zur Kombination von Nanostrukturformationen in Blockcopolymeren mit speziellen Funktionaltäten bieten. Folglich sind die speziellen Eigenschaften dieser Funktionalpolymere wie die Fähigkeit zur Photostrukturierung und Verankerung auf Goldsubstraten für nanotechnologische Anwendungen sehr interessant
Dupont, Tiphaine. "Réalisation de sources laser III-V sur silicium." Phd thesis, Ecole Centrale de Lyon, 2011. http://tel.archives-ouvertes.fr/tel-00604962.
Повний текст джерелаPrandato, Emeline. "Relations structure-propriétés et résistance à l'endommagement de vernis acrylate photo-polymérisables pour substrats thermoplastiques : évaluation de monomères bio-sourcés et de nano-charges." Phd thesis, INSA de Lyon, 2013. http://tel.archives-ouvertes.fr/tel-00995108.
Повний текст джерелаCzerniak, Marlon João 1984, Sidney Luiz 1971 Stürmer, and Universidade Regional de Blumenau Programa de Pós-Graduação em Engenharia Ambiental. "Estudo de resíduos do setor florestal como substrato para produção de inoculante micorrizico on farm e seus efeitos no crescimento de espécies arbóreas /." reponame:Biblioteca Digital de Teses e Dissertações FURB, 2014. http://www.bc.furb.br/docs/DS/2014/358827_1_1.pdf.
Повний текст джерелаKrinke, Thomas Johannes. "Nanopartikel aus der Gasphase: Depositionsmechanismen und strukturierte Anordnung auf glatten Substratoberflächen Nanoparticles from the Gas Phase: Deposition Mechanisms and Structured Arrangements on Flat Substrate Surfaces." Gerhard-Mercator-Universitaet Duisburg, 2002. http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-08132002-124254/.
Повний текст джерелаNicolini, Jaqueline, Vanderlei Gageiro Machado, and Universidade Regional de Blumenau Programa de Pós-Graduação em Química. "Detecção de analitos neutros e aniônicos através do uso de sensores cromogênicos baseados na competição de uma merocianina solvatocrômica e do substrato por uma unidade receptora /." reponame:Biblioteca Digital de Teses e Dissertações FURB, 2006. http://www.bc.furb.br/docs/DS/2006/326911_1_1.pdf.
Повний текст джерелаHillier, Heidi Therese. "How is substrate selectivity in hydride transfer decided in an alcohol dehydrogenase? : Directed evolution of alcohol dehydrogenase A from Rhodococcus ruber DSM 44541 through iterative saturation mutagenesis, a study to understand the structure and function relationship of enzymatic catalysis." Thesis, Uppsala universitet, Biokemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-331683.
Повний текст джерелаGorfu, Paulos. "Untersuchung von Dünnschichtsystemen mittels Elektronenstrahl-Mikroanalyse." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1237375992053-95064.
Повний текст джерелаThe paper deals with the application of the materials analysis method EPMA by peak-to-background ratios, which is currently being used for the analysis of thick samples successfully, to thin layers (less than 1 μm) for the quantitative element analysis as well as for thickness prediction. In addition a model has been established on the Basis of an EPMA method for two films on a substrate for deriving the phase growth coefficient of an inter-metallic phase which grows during the diffusion between a thin layer and a substrate from EPMA measurements while simultaneously heating the sample
Shaik, Md Munan. "Pathogenesis-related Proteins From Helicobacter pylori: Structural and Functional Studies." Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3421716.
Повний текст джерелаIl batterio Helicobacter pylori è riconosciuto essere uno dei più diffusi patogeni umani: esso colonizza circa la metà della popolazione umana, con una elevata velocità di propagazione nei paesi in via di sviluppo. Benché molti soggetti infetti siano asintomatici, una significativa minoranza di questi (15-20%) sviluppano durante la loro vita patologie duodenali gravi, che includono ulcera gastrica e duodenale, adeno-carcinoma e linfoma dello stomaco. Fino ad oggi sono stati completamente sequenziati nove diversi ceppi di H. pylori, in quanto esso presenta una alta variabilità genetica, non solo nelle sequenze geniche, ma anche nel contenuto di geni. Molti dei geni del batterio sono annotati solo sulla base dell’omologia di sequenza e per circa il 45% di essi la funzione è incerta o addirittura ignota. La differenza più significativa tra i ceppi virulenti del batterio rispetto ai ceppi non virulenti è la presenza o assenza della cosiddetta cag-PAI (una sequenza del DNA di 40-kb definita “isola di patogenicità cag”), un inserto genico che codifica per un sistema di secrezione di tipo IV, responsabile della trasloscazione della tossina CagA nelle cellule epiteliali. Benché H. pylori in molti pazienti possa essere sradicato mediante antibiotici, l’aumento della resistenza in alcuni ceppi rappresenta un problema emergente. Nuove terapie sono richieste per combattere il batterio e l’individuazione di nuovi bersagli farmacologici può essere utile per sviluppare nuove strategie di trattamento. Recentemente, nuovi fattori importanti per la colonizzazione e per lo stabilirsi dell’infezione sono stati identificati. In questo lavoro di tesi, un gruppo di queste proteine sono state clonate, espresse in E. coli e purificate allo scopo di effettuare studi strutturali. Obiettivo della tesi era quello di determinare la struttura tridimensionale e caratterizzare la funzione di proteine importanti per la colonizzazione dello stomaco e per la patogenesi. In particolare, gli sforzi sono stati concentrati sugli enzimi coinvolti nella modifica della parete cellulare (peptidoglicano deacetilasi), nella biosintesi di LPS (ADP-L-glycero-D-manno-eptoso-6-epimerasi, rfaD), su una periplasmic-substrate binding protein di un trasportatore ABC (ceuE), su enzimi chiave nel ciclo di assimilazione dell’azoto (glutamina sintasi) e sulla disolfuro ìsomerasi (DsbG), una proteina immunogenica secreta. La strategia applicata è consistita in una analisi bioinformatica preliminare, nell’ottenimento del gene a partire da amplificazione mediante PCR, nella costruzione di un vettore per la clonazione e nell’espressione della proteina in E. coli. Dopo analisi dell’espressione e ottimizzazione delle condizioni, è stata analizzata la solubilità della proteina ricombinante. Quest’ultima è stata quindi purificata mediante diverse tecniche cromatografiche, ed eventualmente caratterizzata per gel-filtrazione analitica, spettrometria di massa, spettroscopia UV. Sono state usate tecniche per rendere i campioni di proteina più adatti per la cristallizzazione, quali DLS (dynamic light scattering) e per investigarne la struttura secondaria, quali CD (dicroismo circolare). La proteina è stata quindi concentrata prima di essere sottoposta ai test di cristallizzazione. I dati di diffrazione sono stati misurati ai sincrotroni ESRF (Grenoble, Francia). La caratterizzazione funzionale delle proteine è stata eseguita usando spettroscopia di fluorescenza, CD, ITC, UV ed ELISA. Dopo una introduzione generale sul batterio (Capitolo 1), nel capitolo 2 viene descritta la struttura tridimensionale e l’attività enzimatica di una putativa peptidoglicano deacetilasi. HP0310 (HpPdgA) da H. pylori è stato indicato come l’enzima responsabile della modifica del peptidoglicano che serve a minimizzare la risposta immunitaria da parte dell’ospite. L’enzima, che appartiene alla famiglia delle polisaccaride deacetilasi, è un omo-tetramero. Le quattro catene polipeptidiche, ciascuna avvolta in un dominio singolo caratterizzato da un TIM-barrel non canonico, sono arrangiate attorno ad un asse di rotazione quaternario. Il sito attivo, uno per monomero, contiene uno ione coordinato in modo simile ad altre deacetilasi. L’enzima non presenta però in vitro attività sui tipici substrati delle peptidoglicano deaetilasi. In netto contrasto con altre peptidoglicano deacetilasi conosciute, HpPdgA non ha un sito di legame accessibile ad una molecola ingombrante quale un polisaccaride, suggerendo che l’enzima leghi nel proprio sito attivo un substrato di piccole dimensioni. Nel capitolo 3 viene discussa in dettaglio la struttura cristallina dell’ultimo degli enzimi del ciclo della biosintesi dell’oligosaccaride (L,D-eptoso) del core di LPS. H. pylori deve molta dell’integrità della sua membrana esterna ai lipopolisaccaridi (LPS). Insieme al loro esenziale ruolo strutturale, gli LPS contribuiscono alle proprietà di aderenze del batterio, come anche alla modulazione della risposta immunitaria. L’oligosaccaride del core del batterio, uno dei tre principali domini dell’LPS, presenta una struttura peculiare nell’organizzazione della ramificazione delle unità che si ripetono. Queste mostrano ulteriore variabilità quando si confrontano ceppi diversi. In questo capitolo viene presentata la struttura cristallina della ADP-L-glicero-D-manno-eptoso-6-epimerasi (HP0859, rfaD), l’ultimo enzima del ciclo che produce L-glicero-D-manno-eptoso partendo da sedoeptuloso-7-fosfato, un composto cruciale nella sintesi dell’oligosaccaride del core. In uno studio recente è stato caratterizzato un mutante knok-out di HP0859 che mostra, in un modello di infezione in cellule AGS, una seria perdita di struttura del lipopolisaccaride e una significativa riduzione dei livelli di adesione, se paragonato ai ceppi wild-type. La struttura cristallina rivela che l’enzima è un omo-pentamero e che NAD è legato come cofattore in una cavità altamente conservata. Il sito di legame del substrato è molto simile a quello del suo ortologo in E. coli, suggerendo anche un simile meccanismo catalitico. Altri enzimi del ciclo sono discussi nei termini della loro struttura tridimensionale. Nel capitolo 4 viene descritta la struttura tridimensionale di una binding-protein al trasportatore periplasmico ABC. E’ noto dalla letteratura che il batterio può utilizzare l’eme come sola sorgente di ferro, e sono state identificate sorgenti di ferro, derivanti dall’ospite, utilizzate da H. pylori, inclusi composti provenienti da gruppi eme da tessuti danneggiati. Il trasporto di eme entro il citoplasma è effettuato da un sistema di trasporto attivo che comprende una proteina periplasmica solubile, una permease citoplasmatica e una ATPase (ABC transporter). La proteina periplasmica che lega l’eme è stata identificata e caratterizzata in vari batteri patogeni, ma fino ad ora non era ancora stata identificata una heme-binding protein in H. pylori. Hp1561 (ceuE) era annotata come una “ABC transporter periplasmic binding protein”, ma un gene omologo from H. mustalae era stato riportato essere coinvolto nel trasporto del nichel. Per chiarire le caratteristiche strutturali di questa putativa proteina di trasporto, essa è stata clonata, espressa e purificata con buona resa in E. coli, cristallizzata e la sua struttura determinata nella forma apo- and in complesso con il Ni(II). La struttura è stata risolta per mezzo di esperimenti di dispersione anomala singola (SAD) su cristalli di Se-metionina. Il modello molecolare è costituito da due domini, collegati da una lunga α-elica e presenta le caratteristiche generali di altre “heme-binding periplasmic ABC transporters” o di “B12 binding-proteins”. Il sito di legame del substrato è localizzato tra i due domini. La struttura cristallina suggerisce che il Ni(II) non è il legante naturale della proteina e che la cavità di legame assomiglia di più a quella delle heme-binding proteins. Sono stati effettuati anche studi di legame in vitro con tecniche diverse (fluorescenza e ITC), che hanno confermato che ceuE in H. pylori è una “periplasmic heme-binding protein”, responsabile per l’assunzione dell’eme. La struttura cristallina di un enzima chiave nell’unico ciclo di assimilazione dell’azoto in H. pylori è discusso nel capitolo 5. La glutamina sintetasi (GS) catalizza la sintesi di glutamina, un intermedio centrale nel metabolismo dell’azoto, da ATP, glutammato e ammoniaca, in una reazione dipendente da un catione bivalente. L’ammoniaca, che è anche una sorgente preferita di azoto per H. pylori, è disponibile in grande quantità, grazie all’attività ureasica del batterio. E’ assimilato in proteine e altri composti contenenti azoto attraverso un singolo ciclo di incorporazione dell’azoto, mediato da GS, un enzima codificato dal gene hp0512. L’assenza di un sito si regolazione allosterico (sito di adenilazione) e di altri enzimi chiave nel ciclo di assimilazione dell’azoto rende HpGS un interessante soggetto per gli studi strutturali, per chiarirne le caratteristiche strutturali e il meccanismo regolatorio. La glutamina sintetasi (HpGS) di H. pylori è stata clonata, espressa, purificata e cristallizzata e la sua struttura determinata. L’enzima è un dodecamero, i cui monomeri sono tenuti assieme soprattutto da interazioni idrofobiche e legami ad idrogeno tra i due anelli esamerici. L’elica N-terminale, chiamata “elica stringa”, è inserita in una cavità idrofobia nella subunità eclissata sull’anello esamerico opposto. In aggiunta, il canale centrale del dodecamero è formato da sei fogli L a quattro fogli beta, ciascuno costituito da un loop antiparallelo cui contribuiscono subunità in anelli opposti. La struttura di un monomero consiste di un piccolo dominio N-terminale e di un dominio C-terminale più grande. L’enzima dodecamerico contiene 12 siti attivi, ciascuno dei quali può essere descritto come un “bifunnel”, in cui ATP e glutammato legano da lati opposti. Il sito di legame dell’ATP è localizzato nella parte alta del bifunnel, alla giunzione del quale ci sono due siti per il legame di cationi bivalenti. Il sito di adenilazione che in tutti gli enzimi omologhi contiene la sequenza consenso NLYDLP è sostituita in H. pylori da NLFKLT (residui da 405 a 410). Poiché la tirosina 407 è il bersaglio conservato dell’adenilazione e H. pylori apparentemente manca di questo residuo, non c’è adenilazione nell’enzima e non si osservano modifiche strutturali significative in questo loop a confronto con altre strutture di GS. Nel capitolo 6 vengono discussi la clonazione, l’espressione e la caratterizzazione della proteina immunogenica secreta DsbG. Le proteine coinvolte nell’isomerizzazione dei ponti disolfuro sono ben note. Esse sono localizzate nelle membrane o nel periplasma e sono chiamate Dsb (Disulfide bond formation). Questi enzimi catalizzano l’introduzione di ponti disolfuro, la loro isomerizzazione o rimozione. Secernere alcune proteine è un modo attraverso il quale un batterio può interagire con l’ospite e in H. pylori questo avviene attraverso uno dei tre sistemi di secrezione di tipo IV presenti. Molte tra le proteine secrete, alcune delle quali risiedono nello spazio periplasmico, formano ponti a disolfuro dopo la traslocazione. HP0231 ha una sequenza simile a Dsbg di E. coli e contiene un motivo CXXC. Essa è stata già identificata come una proteina immunogenica riconosciuta da sieri di pazienti. La proteina ricombinante, espressa in E. coli, non lega fortemente alla resina di affinità IMAC-Ni2+, probabilmente a causa di degradazione dell’His-tag presente all’N-terminale. Per risolvere il problema della purificazione sono perciò stati adottati due approcci: è stato preparato un nuovo costrutto di HP0231 con His-tag al C-terminale, e in parallelo la proteina è stata purificata dai corpi di inclusione e poi rifoldata. La proteina è infine stata purificata ad alta omogeneità. HP0231 è stata caratterizzata usando tecniche immunologiche e blot con sieri di pazienti.
Grzeszczuk, Magdalena. "Thiol-oxidoreductases of Helicobacter pylori – analysis of their interactions with substrates and redox partners." Doctoral thesis, 2018. https://depotuw.ceon.pl/handle/item/3046.
Повний текст джерела