Дисертації з теми "Drug delivery in cancer therapy"
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Yung, Bryant Chinung. "NANOPARTICLE DRUG DELIVERY SYSTEMS FOR CANCER THERAPY." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417614665.
Повний текст джерелаZi, Hong. "Polymers for drug delivery in cancer therapy /." May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Повний текст джерелаLiu, Yang. "Development of Novel Drug Delivery Systems for Cancer Therapy." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu153105342400785.
Повний текст джерелаXu, Leyuan. "Engineering of Polyamidoamine Dendrimers for Cancer Therapy." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3773.
Повний текст джерелаQin, Yiru. "Graphene Quantum Dots-Based Drug Delivery for Ovarian Cancer Therapy." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6358.
Повний текст джерелаMan, Kwun-wai Dede, and 文冠慧. "Oleanolic acid delivery using biodegradable nanoparticles for cancer therapy." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/208550.
Повний текст джерелаEscolà, Jané Anna. "Somatostatin analogues as drug delivery systems for receptor-targeted cancer therapy." Doctoral thesis, Universitat de Barcelona, 2018. http://hdl.handle.net/10803/663804.
Повний текст джерелаLa somatostatina (SST o SRIF14) es una hormona peptídica secretada por el sistema nervioso central y el tracto gastrointestinal que tiene efectos anti-secretores, anti-proliferativos y anti-angiogénicos. Aunque su administración como fármaco es eficaz en ciertas condiciones, su uso terapéutico está limitado por su corta vida media plasmática (<3 min), el amplio espectro de respuestas biológicas y la falta de selectividad entre sus receptores. Con el fin de obtener análogos más estables y selectivos, hemos incorporado aminoácidos aromáticos no naturales ricos y pobres en electrones en posiciones clave de la secuencia nativa para superar dichos inconvenientes. Así, se obtuvieron diferentes análogos que fueron estudiados por RMN obteniendo la estructura de sus conformaciones mayoritarias. También se determinó su perfil de unión a los receptores y sus vidas medias. Entre los análogos, uno destacó por tener una vida media de 40 h, la más alta conocida para un análogo de 14 aminoácidos. Además, mostró un conjunto de conformaciones en solución parecido y una gran selectividad para SSTR2. Recientemente, la terapia contra el cáncer dirigida a receptores ha ganado interés ya que ciertos receptores están sobre-expresados en las células cancerosas. Este es el caso de los receptores de somatostatina en tumores endocrinos. Así, acoplamos diferentes moléculas en la parte N-terminal del análogo mencionado anteriormente. La primera fue un cromóforo que nos permitió seguir la internalización del análogo en dos líneas celulares: CHO-K1 de tipo salvaje (WT) y CHO-K1 con SSTR2 sobre-expresado (ST); dicha internalización fue mucho mejor en ST que en WT. Al ver estos resultados prometedores, fuimos un paso más allá y probamos el análogo cómo sistema de liberación de fármacos, acoplándolo a un cromóforo que cambia de color (verde: unido al péptido, azul: cuando se libera). Cómo antes, tanto la internalización como la liberación fueron mejores en ST que en WT. El último paso fue probar el análogo como inhibidor de p38α acoplando el inhibidor directamente en la parte N-terminal. Cómo en los ensayos anteriores, la inhibición de p-Hsp27 (diana downstream de p38α) fue mejor en ST que en WT, lo que se atribuyó a una mejor internalización del análogo en ST.
Riaz, Muhammad Kashif. "Peptide functionalized drug delivery system for an efficient lung cancer therapy." HKBU Institutional Repository, 2019. https://repository.hkbu.edu.hk/etd_oa/609.
Повний текст джерелаKALAJA, ODETA. "Nanoparticles based delivery System of Flavonoids for Cancer Therapy." Doctoral thesis, Università degli Studi di Trieste, 2018. http://hdl.handle.net/11368/2917683.
Повний текст джерелаCheng, Yu. "Gold Nanoparticles as Drug Delivery Vectors for Photodynamic Therapy of Cancers." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1301503263.
Повний текст джерелаYin, Hongran. "RNA nanoparticles for anti-oncogenic miRNA and drug delivery for cancer therapy." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1589836157141191.
Повний текст джерелаMadadi, Ardekani Sara. "CARBON NANOMATERIALS DERIVED FROM ORGANIC SOURCES FOR DRUG DELIVERY AND THERAPY." Thesis, The University of Sydney, 2019. http://hdl.handle.net/2123/20998.
Повний текст джерелаHeister, Elena. "Functionalized carbon nanotubes as a multimodal drug delivery system for target cancer therapy." Thesis, University of Surrey, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529424.
Повний текст джерелаGuo, Sijin. "RNA Nanoparticle as A Safe and Effective Drug Delivery Platform for Cancer Therapy." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1561719043509709.
Повний текст джерелаStolzoff, Michelle L. "Designing the surface properties of expansile nanoparticles for targeted cancer therapy." Thesis, Boston University, 2013. https://hdl.handle.net/2144/21256.
Повний текст джерелаNanoparticle-based drug delivery has been explored to circumvent the often-toxic chemotherapy treatments used today by providing a more efficient and specific delivery to diseased tissues. Recently we have developed polymeric pH-responsive expansile nanoparticles (eNPs) for intracellular delivery of paclitaxel (Pax) as an improvement upon the traditional methods of delivery of Pax with using Cremophor/ethanol. As eNPs are internalized by the cell, the hydrophobic protecting groups found on side chains along the polymer backbone are hydrolyzed, leaving behind hydrophilic moieties that cause the eNPs to slowly swell with water. In this manner, the encapsulation and controlled release of a hydrophobic drug can be achieved. By altering the surface characteristics of the eNPs, one can change the behavior of the delivery vehicle as well as the biological response. To explore this approach, two surfactant strategies were employed. Specifically, the original sodium dodecyl sulfate (SDS) surfactant has been substituted with PEGylated surfactants (either lipids or poloxamer) to improve circulation and in vivo stability. In addition, these surfactants were functionalized to target the folate receptor (FR), which is overexpressed in several cancers, in order to increase cancer cell-specific localization and uptake. The resulting eNPs retained their swelling characteristics while demonstrating improved cellular uptake in folate receptor-expressing KB and MDA-MB-231 carcinoma cells with no change in uptake in A549 cells, which do not express the folate receptor.
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García, Díaz María. "Drug delivery in photodynamic therapy: From pharmaceutics to animal testing." Doctoral thesis, Universitat Ramon Llull, 2012. http://hdl.handle.net/10803/81987.
Повний текст джерелаSe ha estudiado el desarrollo de fotosensibilizadores y su formulación en terapia fotodinámica. Se han caracterizado las propiedades fotofísicas de los fotosensibilizadores porficénicos. Se han propuesto diferentes estrategias tales como la introducción de grupos carboxilato en la periferia o iones de metales pesados en el núcleo, para mejorar el diseño de nuevos fotosensibilizadores basados en el macrociclo porficénico. Entre ellos, el temoceno (m-THPPo), el porficeno análogo a la temoporfina, muestra excelentes propiedades fotofísicas, fotoestabilidad y alta eficacia fotodinámica. Debido a su alta hidrofobicidad, se ha desarrollado una formulación liposomal para la administración in vitro e in vivo del temoceno. m-THPPo/DPPC/DMPG (1:67.5:7.5 relación molar) tiene alta eficiencia de encapsulación manteniendo sus propiedades tanto fotofísicas como biológicas. El temoceno liposomal exhibió la eficacia fotodinámica in vitro más alta por molécula internalizada, siendo un sistema de administración de fármacos eficaz para una estrategia in vivo dirigida a las células tumorales. El temoceno encapsulado en micelas de Cremophor EL mostró una mínima internalización celular. Consistentemente, la formulación micelar mostró mejor la respuesta in vivo cuando se utiliza en un régimen vascular. Con el fin de minimizar la internalización del fotosensibilizador en las células normales, se propusieron liposomas decorados con ligandos folato. Esta estrategia resulta en una internalización dos veces mayor de los liposomas dirigidos al receptor folato respecto a la correspondiente formulación no específica. Por último, han sido explorados nuevos modelos celulares in vitro para la optimización de los procesos con oxígeno singlete. Los cultivos celulares en 3D reproducen la heterogeneidad de oxígeno y fotosensibilizador que está presente en los tejidos reales, proporcionando información muy útil para interpretar y predecir el resultado de la terapia fotodinámica. También se ha demostrado la capacidad de desactivación del oxígeno singlete de antioxidantes en un modelo ex vivo de piel porcina.
The photosensitizer and formulation development in photodynamic therapy have been studied. They have been characterized the photophysical properties of new porphycene-based photosensitizers. Different strategies such as the introduction of carboxylate groups in the periphery or heavy metal ions in the core have been proposed for improving the design of novel photosensitizers based on the porphycene macrocycle. Among them, temocene (m-THPPo), the porphycene analogue to temoporfin, shows excellent photophysical properties, superior photostability and high photodynamic efficiency. Owing to its high hydrophobicity, a liposomal formulation has been developed for in vitro and in vivo administration of temocene. m-THPPo/DPPC/DMPG (1:67.5:7.5 molar ratio) yielded high encapsulation efficiency maintaining its photophysical and biological properties. Liposomal temocene exhibited the highest in vitro killing efficacy per uptaken molecule and they were an efficient drug delivery system for in vivo tumor cell targeting strategy. Temocene encapsulated in Cremophor EL micelles showed minimal cell internalization. Consistently, micellar formulation showed the best in vivo response when used in a vascular regime. In order to minimize the internalization of the photosensitizer in normal cells, liposomes decorated with folic acid ligands were proposed. This strategy leads to a 2-fold higher uptake of folate-targeted liposomes than the corresponding non-targeted formulation. Finally, new in vitro cellular models for a better optimization of singlet oxygen-involved processes were explored. 3D cellular cultures reproduced the oxygen and photosensitizer heterogeneity found in real tissues, providing useful information to interpret and predict the photodynamic therapy outcome. The singlet oxygen quenching ability of antioxidants in ex vivo porcine skin model has also been demonstrated.
Adjei, Isaac Morris. "Nanoparticle-mediated cancer therapy for primary and metastasized tumors." Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1386342707.
Повний текст джерелаGade, Terence Peter Ferrante. "Integrated imaging of drug delivery : a molecular imaging approach to the optimization of cancer therapy /." Access full-text from WCMC:, 2007. http://proquest.umi.com/pqdweb?did=1432803381&sid=12&Fmt=2&clientId=8424&RQT=309&VName=PQD.
Повний текст джерелаGubbins, James. "Engineering theranostic liposomes for image guided drug delivery as a novel nanomedicine for cancer therapy." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/engineering-theranostic-liposomes-forimage-guided-drug-delivery-as-a-novelnanomedicine-for-cancer-therapy(ce8381bb-84ee-4b9a-a96c-d09b21956c73).html.
Повний текст джерелаYu, Bo. "Oligonucleotide Based Liposomal Nanoparticles for Leukemia and Liver Cancer Therapy." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1275490450.
Повний текст джерелаShao, Huimin. "Development of an advanced drug delivery system to prevent and treat breast cancer bone metastasis." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/28640.
Повний текст джерелаErebor, Joan Onyebuchi. "Synthesis and evaluation of targeted dendrisomes as novel gene and drug delivery systems for cancer therapy." Thesis, University of Strathclyde, 2018. http://digitool.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=30187.
Повний текст джерелаGiesen, Beatriz [Verfasser], and Ulf Dietrich [Gutachter] Kahlert. "Gold Nanoparticles as Drug Delivery Systems for Brain Cancer Therapy / Beatriz Giesen ; Gutachter: Ulf Dietrich Kahlert." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2021. http://d-nb.info/1237883814/34.
Повний текст джерелаTraore, Mahama Aziz. "Bacteria-Enabled Autonomous Drug Delivery Systems: Design, Modeling, and Characterization of Transport and Sensing." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/64326.
Повний текст джерелаPh. D.
Dalela, Manu. "Synthesis, characterization and biological evaluation of poly(styreneco-maleic anhydride) as a drug delivery vehicle for cancer therapy." Thesis, IIT Delhi, 2016. http://localhost:8080/xmlui/handle/12345678/7037.
Повний текст джерелаBABANYINAH, GODWIN KWEKU. "Theranostic Nanoparticles Folic acid-Carbon Dots-Drug(s) for Cancer." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/asrf/2021/presentations/40.
Повний текст джерелаBabanyinah, Godwin Kweku. "Theranostic Nanoparticles Folic Acid-Carbon Dots-Drug(s) for Cancer." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/etd/3892.
Повний текст джерелаFarvadi, F., A. M. Tamaddon, and F. Hashemi. "PEG-grafted Hyperbranched Polyethyleneimine-Oxidized Single Walled Carbon Nanotube Complex (PEG-PEI-SWNT) for Sustained Delivery of Doxorubicin." Thesis, Sumy State University, 2012. http://essuir.sumdu.edu.ua/handle/123456789/34928.
Повний текст джерелаMartínez, Edo Gabriel. "Radial-capped mesoporous silica nanoparticles for multiple drug delivery." Doctoral thesis, Universitat Ramon Llull, 2020. http://hdl.handle.net/10803/669168.
Повний текст джерелаEn la presente tesis doctoral, se ha desarrollado un sistema de liberación de fármacos sensible a pH basado en nanopartículas mesoporosas de sílice (MSN). Los poros de la nanopartícula están radialmente obstruidos mediante la funcionalización de cadenas de PEG, sustituidas con un fármaco en uno de sus extremos. El objetivo de esta nueva metodología es la de preservar la carga interna de estas MSN. En primer lugar, se ha estudiado el concepto de obstrucción radial para evaluar la utilidad práctica de este método. Por esta razón, diferentes tipos de cadenas de PEG con carga, a saber, aminas cuaternarias y PEG neutrales, se han funcionalizado sobre la MSN para estudiar su capacidad de obstrucción. Como prueba de concepto, se ha estudiado la liberación de safranina en medio fisiológico (pH 7.4). Los resultados obtenidos han demostrado que las cadenas de PEG que contienen una carga positiva obstruyen mejor los poros que las cadenas de PEG neutrales de la misma longitud. Utilizando esta aproximación, se ha diseñado un sistema de liberación de fármacos para la vehiculización de camptotecina (CPT) y topotecán (TPT). En primer lugar, uno de los anteriores fármacos se ha adsorbido dentro de los poros de la MSN. Posteriormente, los poros se han sellado mediante una cadena de PEG que contiene doxorrubicina (DOX) en uno de sus extremos (DOX-PEG). La estabilidad de dicho sistema en condiciones fisiológicas prueba la eficacia de la obstrucción radial. Por otro lado, en condiciones ácidas, se produce una liberación descontrolada de los fármacos. Asimismo, los experimentos de citotoxicidad in vitro han demostrado que el sistema puede liberar CPT y DOX en las células cancerígenas HeLa, logrando un mayor efecto sinérgico que la combinación de TPT y DOX. También se ha sintetizado un profármaco de la CPT con el objetivo de aumentar su carga en una MSN y mejorar así su efecto sinérgico con la DOX. Para llevarlo a cabo, se ha unido una cadena escindible de PEG a la CPT. Empleando esta estrategia se ha conseguido cargar un 30% más de CPT en el interior de las MSN. El sistema muestra una gran estabilidad en condiciones fisiológicas, ya que se observa una liberación negligible de los fármacos. Además, se ha evaluado la citotoxicidad del sistema en dos líneas celulares diferentes: HeLa y HepG2. Los resultados obtenidos demuestran que el nuevo profármaco sintetizado en combinación con DOX, resulta en un mayor efecto sinérgico en las células HepG2. Por otro lado, la selectividad de las MSN hacia las células HepG2 se ha mejorado mediante la introducción del ligando ácido glicirretínico (GA) sobre el grupo DOX-PEG. Para llevarlo a cabo, se ha empleado la misma aproximación radial establecida para los otros sistemas. Los estudios de internalización celular han demostrado que este nuevo sistema es capaz de discriminar entre las células HeLa y HepG2, acumulándose preferentemente en estas últimas. Finalmente, se ha evaluado un sistema de administración triple de medicamentos con el objetivo de superar el efecto de resistencia de los tumores a múltiples fármacos. Esta acción se puede emprender mediante la combinación de medicamentos quimioterapéuticos, DOX y CPT, con un agente fototerapéutico (ftalocianina). Por esta razón, se ha sintetizado un nuevo conjugado de la CPT con una ftalocianina. Dicho conjugado se ha cargado dentro de los poros de la MSN y posteriormente se han sellado con el grupo DOX-PEG. Los experimentos de internalización celular han demostrado la endocitosis de este sistema en las células HeLa y la posterior liberación de los fármacos. Asimismo, se ha evaluado in vitro el efecto sinérgico entre la DOX y la CPT.
In this PhD dissertation, a pH-responsive multiple drug delivery system (DDS) based on mesoporous silica nanoparticles (MSN) with a radial-capping of its pores has been developed. This is a new concept that relies on the functionalization of the particle surface with PEG chains substituted with a drug at its end to preserve the inner cargo of the MSN. First, the concept of radial-capping has been studied to assess the practical usefulness of such capping method. Thus, different types of charged PEGs, namely quaternary amines and neutral PEGs, have been introduced upon an MSN in order to study its capping ability. As a proof of concept, the dye safranin was loaded into the nanoparticles pores, which were subsequently capped with PEGs chains. Then, the release of safranin was assessed under physiological conditions (pH 7.4). The results obtained demonstrated that PEG chains possessing positive charge provides a more efficient capping than the neutral PEGs of the same length. Using this approach, a drug delivery system (DDS) based on the radial capping for the delivery of camptothecin (CPT) and topotecan (TPT) has been studied. CPT or TPT has been loaded within the pores of an MSN, and subsequently sealed with a PEG chain decorated with doxorubicin (DOX) at its end (DOX-PEG moiety). The system is stable under physiological conditions (pH 7.4) which confirms the effectiveness of the radial capping. On the other hand, under acidic pH, a burst release of drugs takes place. Furthermore, the in vitro cytotoxicity test has demonstrated that this DDS can effectively deliver CPT and DOX to HeLa cells achieving a better synergistic effect than the combination of TPT and DOX. With the aim to improve the loading of CPT to enhance the synergistic effect with the latter system (DOX-PEG moiety), a prodrug of CPT has been synthesised. To do so, a cleavable reductive short PEG chain has been bonded to CPT. An increase of loading of 30% has been achieved in comparison with the unmodified drug. The stability of the radial-capping methodology has been tested as mentioned above. Under physiological conditions, the release of drugs is negligible. The cytotoxicity activity of the system has been tested in two different cell lines: HeLa and HepG2 cells. The results showed a better synergistic effect of this new synthesised system towards HepG2 cells. In order to further improve the selectivity of the system towards HepG2 cells, the MSN were decorated with glycyrrhetinic acid (GA) ligand over the DOX-PEG moiety. Uptake studies have shown that this new system preferably accumulates in HepG2 cells in comparison to HeLa cells. Finally, a tri-deliver system of drugs has been developed with the aim to try to overcome the multiple drug-resistant (MDR) effect by the combination of chemotherapeutic drugs (DOX and CPT) with a phototherapeutic agent (phthalocyanine). In this regard, a new CPT conjugate with a phthalocyanine has been synthesised and loaded within the pores of an MSN. Then, the system has been sealed with the DOX-PEG moiety. The uptake studies have demonstrated the proper endocytosis of the system inside HeLa cells and the subsequent delivery of the three drugs in the cytoplasm and nucleus. Furthermore, the synergistic effect of DOX and CPT has been assessed in vitro.
Guerrant, William. "Targeted histone deacetylase inhibition." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/44907.
Повний текст джерелаNadal, Bufi Ferran. "Peptide-based drugs to inhibit LDH5, a potential target for cancer therapy." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/232526/1/Ferran_Nadal%20Bufi_Thesis.pdf.
Повний текст джерелаSchilb, Andrew L. "OPTIMIZATION OF NON-VIRAL GENE DELIVERY SYSTEM FOR IMAGE-GUIDED THERAPY FOR TRIPLE NEGATIVE BREAST CANCER." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1627484657204883.
Повний текст джерелаFoy, Susan Patricia. "Multifunctional Magnetic Nanoparticles for Cancer Imaging and Therapy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=case1319836040.
Повний текст джерелаPeralta, Donna V. "Synthesis and Applications of Mutimodal Hybrid Albumin Nanoparticles for Chemotherapeutic Drug Delivery and Phototherml Therapy Platforms." ScholarWorks@UNO, 2014. http://scholarworks.uno.edu/td/1886.
Повний текст джерелаLahori, Deeksha. "Combination of nitric oxide and polymer based drug delivery system to improve the effectiveness of a peptide inhibitor in cancer therapy outcomes." Thesis, University of Sydney, 2020. https://hdl.handle.net/2123/24311.
Повний текст джерелаVERDERIO, PAOLO. "Synthesis and Biofunctionalization of Novel Composite Nanocarriers for Targeted Detection and Treatment of Malignant Cells." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2014. http://hdl.handle.net/10281/50789.
Повний текст джерелаHolden, Christopher A. "MODIFIED PAMAM DENDRIMERS IN TUNABLE DRUG-DELIVERY SYSTEMS: A SUSTAINED-RELEASE DENDRIMER HYDROGEL FOR ANTI-GLAUCOMA DRUGS AND SURFACE-ENGINEERED MACROPHAGES AS NANOPARTICLE CARRIERS FOR TARGETED ANTI-CANCER THERAPY." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/5038.
Повний текст джерелаHervault, A. M. M. "Development of a doxorubicin-loaded dual pH- and thermo-responsive magnetic nanocarrier for application in magnetic hyperthermia and drug delivery in cancer therapy." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1566981/.
Повний текст джерелаMott, Landon Alexander. "TOWARDS THE RATIONAL DESIGN AND APPLICATION OF POLYMERS FOR GENE THERAPY: INTERNALIZATION AND INTRACELLULAR FATE." UKnowledge, 2019. https://uknowledge.uky.edu/cme_etds/99.
Повний текст джерелаWei, Tuo. "Systèmes innovants de délivrance de médicaments basés sur des nanomicelles pour le traitement du cancer." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4031.
Повний текст джерелаPoor tumor penetration and high toxicity of anticancer drugs, together with the developed drug resistance constitute challenging hurdles for cancer therapy. The application of nanotechnology for anticancer drug delivery is expected to address these issues and bring new hope for cancer treatment. In the first part of my PhD thesis, we used a new tumor-penetrating peptide, CRGDK, to conjugate onto the surface of doxorubicin encapsulated DSPE-PEG2000 nanomicelles. The CRGDK peptide conjugated on the nanomicelles triggered specific binding to Nrp-1 receptors, leading to enhanced cellular uptake and anticancer activity in vitro. The in vivo results further confirmed that the CRGDK-decorated nanomicelles could efficiently accumulate and penetrate into deeper tumors. In the second part of my PhD thesis, we established an original nanomicellar drug delivery system based on an amphiphilic dendrimer (AmDM), which could generate supramolecular micelles to effectively encapsulate the anticancer drug doxorubicin (DOX) with high drug loading capacity (> 40%), thanks to the unique dendritic structure creating large void space for drug accommodation. The resulting AmDM/DOX nanomicelles are able to specifically accumulate at tumor sites via EPR effect and penetrate deeper into tumor tissues thanks to their small size. Most importantly, these nanomicelles exhibit significantly improved anticancer activity and reduced systemic toxicity, and are very effective even towards drug resistant cancers by virtue of their macropinocytotic cell uptake mechanism and their ability to bypass cell drug efflux pumps
Bon, Romero Ignacio. "Development and characterization of a new hydrogel for endoscopic resection. Efficacy as a drug-delivery platform for local therapy in colorectal cancer and experimental colitis animal models." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667190.
Повний текст джерелаEndoscopy is a minimally invasive procedure that allows diagnosing conditions inside the gastrointestinal, respiratory or urinary tract, by means of an endoscope which is inserted through a body passageway. Advances in endoscopic medicine have led to the development of therapeutic endoscopy that enables physicians to treat numerous conditions using endoscopic techniques such as the removal of polyps and early tumors. Endoscopic resection of large lesions leads to extensive mucosal defects and submucosal exposure, with a substantial risk of complications (haemorrhage, stenosis and perforation), as our current mechanical and thermal approaches are ineffective in the closure and resolution of injuries produced by endoscopic resection. On the other hand, there is a growing demand for endoscopic procedures secondary to colorectal detection programs, due to the increasing incidence of colorectal cancer (CCR) and intestinal inflammatory disease (IBD). Moreover, there is no solution for sustained administration of bioactive therapies for the local treatment of inflammatory or tumoral lesions. To solve these needs of therapeutic endoscopy we have developed a new hydrogel, Covergel, which is applied directly through the endoscope on mucous lesions after endoscopic therapeutic procedures. Covergel has demonstrated solid healing properties in preclinical models. Covergel is biocompatible, biodegradable and bioactive; it has an appropriate viscosity and adhesion capacity, it can be easily applied through the endoscope and promotes physiological re-epithelialization. This composition has been developed looking for the substances and their proportions that would improve viscosity, adherence and resistance to bacterial degradation. The resulting optimized solution presents the interesting property of becoming a gel when it comes into contact with the mucous membranes, which is achieved by increasing its viscosity with temperature. In its gel form, the hydrogel can remain attached to the mucosa for a long period of time (at least 36 hours), acting as well as a shield to avoid thermal injuries after EMR or ESD. We have obtained positive results in studies of evidence of lesions induced by thermal damage derived from EMR in rats and pigs that show a statistically significant efficacy in the cure of the mucosa of the new hydrogel. In addition, we have also tested the ability of sustained drug administration of our hydrogel, acting as a drug-delivery platform to administer small molecules and monoclonal antibodies to locally treat mucous injuries in CRC or IBD. The results obtained have been promising since, not only have we shown that the application of a drug-delivery platform through the endoscope is possible but is also effective in the resolution of diseases in animal models tested. This study involves the creation of a new hydrogel to improve therapeutic endoscopy and provide it with new tools to increase the cases in which it is possible to use endoscopy thus avoiding other more invasive therapeutic procedures.
Croissant, Jonas. "NANOMÉDECINE THÉRANOSTIQUE ACTIVÉE À DEUX-PHOTONS POUR LE TRAITEMENT DU CANCER." Thesis, Montpellier, Ecole nationale supérieure de chimie, 2014. http://www.theses.fr/2014ENCM0004/document.
Повний текст джерелаTwo-photon actuated nanomedicine has become one of the main proponents for the achievement of the spatiotemporal selectivity needed for nanomedicine. Indeed, the raison d'être of the medical application of nanotechnology in the field of cancer treatment is to lower and suppress the side effects caused by current techniques such as chemotherapy and radiotherapy, due to their lack of selectivity. Among various nanoparticles (NPs), mesoporous silica nanoparticles (MSN) have attracted increasing attention over the past decade for their low cytotoxicity, cellular internalization and excretion, and the ability to carry multiple features for both the diagnosis and therapy of cancers in a single nanovehicle: the so-called theranostic nanomedicine.In this dissertation, I will describe MSN for one and/or two-photon-actuated fluorescence imaging, drug-delivery, gene delivery and photodynamic therapy (PDT). First, plasmonically-triggered cargo delivery via MSN nanovalves and designed mesoporous silica photodegradation is presented. Then, in-vitro two-photon-triggered drug delivery with azobenzene-functionalized MSN such as nanoimpellers and fluorescent nanovalves, along with preliminary studies of gene delivery via ammonium-functionalized nanoimpellers are discussed. Multifunctional MSN incorporating a two-photon photosensitizer are systematically studied in terms of the resulting optical and photophysical properties of the NPs, and then used for in-vitro biomedical applications.Furthermore, two kinds of emerging nanomaterials are also designed for two-photon actuated nanomedicine, bridged silsesquioxane (BS) and periodic mesoporous organosilica (PMO) NPs. These nanomaterials are elaborated without silica precursor (e.g. tetraethoxysilane) and solely with bis- or tetra-organoalkoxysilanes, thus providing materials with the highest organic content for the targeted applications. Consequently, disulfide-based hybrid BS and PMO NPs were elaborated as biodegradable nanomedical tools, and photosensitizer-based BS and PMO NPs were used for efficient in-vitro PDT. BS and gold-BS core-shells NPs are constructed for ultrabright two-photon imaging and efficient PDT, while two-photon functionalized PMO NPs serve as theranostic nanocarriers. Besides, versatile multipodal ethylene-benzene PMO NPs with very high surface areas are presented as a promising strategy for the design of structural complexities at the nanoscale.Finally, iron oxide core MSN shell (Fe3O4@MSN) nanocontainers are described for versatile applications. The design of two-photon-sensitive magnetic MSN and PMO core-shell nanovehicles is presented as a perspective for gene delivery and magnetic resonance imaging. Furthermore, Fe3O4@MSN containers are constructed for heavy metal removal of twelve of the most toxic metal ions through the diethylene triamine pentaacetic acid (DTPA) ligand. The enhancement of the pollutant removal efficiency is studied by selective surface and/or porous DTPA functionalizations
Champeau, Mathilde. "Dissolving microneedles for an optimal transdermal delivery of an active principle used in photodynamic therapy : development and proof of concept." Thesis, Lille 2, 2020. http://www.theses.fr/2020LIL2S007.
Повний текст джерелаNon-melanoma skin cancers are on the rise with 2 to 3 million people diagnosed each year and are sometimes treated by local ablation therapy. To avoid this surgery, photodynamic therapy (PDT) appears as an advantageous treatment. Currently used in clinics, PDT consists of applying a cream containing a photosensitive precursor to the damaged skin, which, then metabolizes and under light excitation inducescell death. However, this technique is not fully effective if the skin lesion extends into the deep skin layers. To improve the therapeutic treatment of this type of skin cancer, a patch with dissolving microneedles (MNs) was develop to reach the deep layers of lesions that are difficult to treat. Hyaluronic acid, known for its biocompatibility, solubility and biodegradability, was chosen as the constituent material, and mixed with the 5-aminolevulinic acid (photosensitive precursor, 5-ALA). To ensure the best penetration without causing pain by touching the nerve endings, an optimal “pencil-tip” design was defined with MNs length going from 400 to 750μm. A simple and robust manufacturing process called solvent casting molding method, has been set up which is an asset for potential industrialization. In absence of realistic skin lesions model,we chose to establish one on rats skin by applying daily UV-B doses. Histology and pharmacokinetic studies validated the presence of precancerous skin lesions and the MN-patch in vivo efficiency was therefore tested. After one hour application on the injured rat skin, the MN-patch dissolved and released the 5-ALA that further metabolized to protoporphyrin IX (PpIX). A significant level of PpIX fluorescence was recorded suggesting that after light excitation, a PDT session could be effective. In parallel, to reduce pain felt during PDT treatment, a light device with suitable optical and thermal properties was conceived and coupled to the MN-patch. The idea would be to start the illumination directly after MN-patch application in order to avoid a painful photochemical reaction. This wearable and easy to use system purpose a all-in-one PDT processing which fulfills the criterion of patient compliance, better efficiency and speed of treatment
Blaudszun, André-René [Verfasser], and Gerhard [Akademischer Betreuer] Wenz. "Ex vivo activated human T lymphocytes as living drug delivery vehicles for cancer therapy : in vitro studies with nanoparticulate idarubicin and complexed mTHPP as payload / André-René Blaudszun. Betreuer: Gerhard Wenz." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2016. http://d-nb.info/1100061568/34.
Повний текст джерелаThomas, Sean Casey. "A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model." Thesis, Virginia Tech, 2020. http://hdl.handle.net/10919/100772.
Повний текст джерелаMaster of Science
Treating glioblastoma multiforme (GBM), a form of cancer found in the brain, has not been very successful; patients rarely live two years following diagnosis, and there have been no major breakthrough advances in treatment to improve this outlook for decades. We have been working on two treatments which we hope to combine. The first is high-frequency electroporation (H-FIRE), which uses electrical pulses to kill GBM cells while leaving healthy cells alive and blood vessels intact. The second is QUAD-CTX, which combines a toxin with two types of protein that attach to other proteins that are more common on the surface of GBM cells than healthy cells. We have shown these to be effective at disproportionately killing human GBM cells growing in a lab setting. Before H-FIRE and QUAD-CTX may be tested on humans, we need to show them to be effective in an animal model, specifically rats. I have chosen rat glioma cells that will behave similarly to human GBM and a rat species that will not have an immune response to them. I have made these cells bioluminescent so that we may monitor the tumors as they grow and respond to our treatments. I have also shown that QUAD-CTX kills these rat glioma cells, as does H-FIRE. Because of this work, we are ready to begin testing these two treatments in rats.
Babatunde, Oluwaseun Oyeniyi. "Exploring the potential of Rhodobacter sphaeroides in photodynamic therapy of tumors." Bowling Green State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1624793446693196.
Повний текст джерелаBabatunde, Oluwaseun Oyeniyi. "Exploring the potential of Rhodobacter sphaeroides in photodynamic therapy of tumors." Bowling Green State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1624793446693196.
Повний текст джерелаBretin, Ludovic. "Thérapie photodynamique (PDT) dans un modèle in vitro et in vivo de cancer colorectal : utilisation d'un photosensibilisateur nanovectorisé." Thesis, Limoges, 2019. http://www.theses.fr/2019LIMO0052/document.
Повний текст джерелаColorectal cancer (CRC) is one of the most common cancer globally but above all the second leading cause ofdeath for oncological reasons. Despite medical research advances in anti-cancer treatments, many side effectspersist in patients as well as development of resistances to conventional treatments. The development of new anticancertherapeutic strategies is necessary in order to improve care of patients. Photodynamic therapy (PDT) usingphotosensitizers (PS) comes as an innovative therapeutic strategy severely restricting these undesirable sideeffects. PDT has been approved for treatment of some cancers due to the generation of cytotoxic reactive oxygenspecies only with photoactivated PS. However, low physiological solubility and lack of selectivity towards tumorsites are the main limitations of their clinical use. Indeed, targeted drug delivery is a crucial point in cancer therapy.Nanomedicine through the use of nanoparticles improves tumor-targeting because they are able to spontaneouslyaccumulate in solid tumors through an enhanced permeability and retention effect. The purpose of this study wasto prove added value of 5-(4-hydroxyphenyl)-10,15,20-triphenylporphyrin-xylan (TPPOH-X) vectorization bysilica nanoparticles (SNPs) in order to enhance anti-cancer efficacy through better tumor-targeting. It has beendemonstrated significant anti-cancer efficacy increase of TPPOH-X SNPs-PDT thanks to cellular uptakeimprovement relative to free TPPOH-PDT in 3 human CRC cell lines. Moreover, it has been characterized thatcell death induced by TPPOH-X SNPs-PDT is conducted via apoptosis and autophagy acts as a resistance pathwayto cell death. Furthermore, in vivo and without toxicity, TPPOH-X SNPs-PDT induce an elevated anti-cancerefficacy through improvement of tumor-targeting compared to free TPPOH-PDT. This study therefore highlightedthe added value of PDT and nanomedicine combination in order to improve future cancer treatments
Liu, Juan. "Nouveaux systèmes nanométriques et ph dépendant pour le transport de médicaments contre les phénomènes de résistances." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4072.
Повний текст джерелаDrug resistance presents a great hurdle to cancer treatment. Nanotechnology-based drug delivery systems (NDDSs) are widely expected to bring new hope for cancer therapy to overcome drug resistance by specifically delivering anticancer drugs to tumor lesions via the EPR effect, hence increasing local drug concentrations and consequently enhancing therapeutic efficacy, and at the same time, sparing healthy tissues to avoid side effects. As tumors often have an acidic microenvironment, we would like to further endow the NDDS with a pH-responsive drug releasing property for specific tumor targeting. In this thesis, we established different pH-responsive NDDSs by employing different strategies. These NDDSs could specifically control drug release at tumor tissues and within tumor cells in response to acidic pH. By increasing the intracellular drug concentration, the goal of circumventing drug resistance in cancer was achieved. The present study provides new insights into the design of nanocarriers to overcome drug resistance through pH-responsive drug delivery, and illustrates how advances in nanotechnology can be advantageously implemented to enhance therapeutic outcomes
Franke, Christina E. "Tobacco Mosaic Virus Nanocarrier for Restored Cisplatin Efficacy in Platinum-Resistant Ovarian Cancer." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1493810190306879.
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