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Статті в журналах з теми "Droplet Recombinase Polymerase Amplification"
Schuler, Friedrich, Frank Schwemmer, Martin Trotter, Simon Wadle, Roland Zengerle, Felix von Stetten, and Nils Paust. "Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA." Lab on a Chip 15, no. 13 (2015): 2759–66. http://dx.doi.org/10.1039/c5lc00291e.
Повний текст джерелаCui, Johnson Q., Frank X. Liu, Hojeong Park, Ka Wai Chan, Tyler Leung, Ben Zhong Tang, and Shuhuai Yao. "Droplet digital recombinase polymerase amplification (ddRPA) reaction unlocking via picoinjection." Biosensors and Bioelectronics 202 (April 2022): 114019. http://dx.doi.org/10.1016/j.bios.2022.114019.
Повний текст джерелаIbekwe, Mark A., Shelton E. Murinda, Stanley Park, Amarachukwu Obayiuwana, Marcia A. Murry, Gregory Schwartz, and Trygve Lundquist. "Comparative Use of Quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and Recombinase Polymerase Amplification (RPA) in the Detection of Shiga Toxin-Producing E. coli (STEC) in Environmental Samples." Water 12, no. 12 (December 13, 2020): 3507. http://dx.doi.org/10.3390/w12123507.
Повний текст джерелаChowdhury, Rajashree, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain, Khaledul Faisal, Rupen Nath, James Baker, et al. "Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis." Tropical Medicine and Infectious Disease 5, no. 2 (June 5, 2020): 95. http://dx.doi.org/10.3390/tropicalmed5020095.
Повний текст джерелаDaher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples." Clinical Chemistry 60, no. 4 (April 1, 2014): 660–66. http://dx.doi.org/10.1373/clinchem.2013.213504.
Повний текст джерелаDaher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Recombinase Polymerase Amplification for Diagnostic Applications." Clinical Chemistry 62, no. 7 (July 1, 2016): 947–58. http://dx.doi.org/10.1373/clinchem.2015.245829.
Повний текст джерелаTomar, Saurabh, Barbora Lavickova, and Carlotta Guiducci. "Recombinase polymerase amplification in minimally buffered conditions." Biosensors and Bioelectronics 198 (February 2022): 113802. http://dx.doi.org/10.1016/j.bios.2021.113802.
Повний текст джерелаNair, Gayatri, Juan David Ramírez, A. Clinton White, Alejandro Castellanos-Gonzalez, A. Elizabeth Pinilla, Mauricio Rebolledo, M. Consuelo López, R. Rebecca Richards-Kortum, and Zachary Crannell. "Detection of Entamoeba histolytica by Recombinase Polymerase Amplification." American Journal of Tropical Medicine and Hygiene 93, no. 3 (September 2, 2015): 591–95. http://dx.doi.org/10.4269/ajtmh.15-0276.
Повний текст джерелаHiggins, Matthew, Matt Ravenhall, Daniel Ward, Jody Phelan, Amy Ibrahim, Matthew S. Forrest, Taane G. Clark, and Susana Campino. "PrimedRPA: primer design for recombinase polymerase amplification assays." Bioinformatics 35, no. 4 (August 8, 2018): 682–84. http://dx.doi.org/10.1093/bioinformatics/bty701.
Повний текст джерелаLobato, Ivan Magriñá, and Ciara K. O'Sullivan. "Recombinase polymerase amplification: Basics, applications and recent advances." TrAC Trends in Analytical Chemistry 98 (January 2018): 19–35. http://dx.doi.org/10.1016/j.trac.2017.10.015.
Повний текст джерелаДисертації з теми "Droplet Recombinase Polymerase Amplification"
Daher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.
Повний текст джерелаThis dissertation consists of an exhaustive study on an emerging technology for isothermal amplification of nucleic acids called recombinase polymerase amplification (RPA). The introduction of this thesis is a detailed description of the RPA. This review documents and discusses the various applications of this technology by pointing to the current knowledge about RPA for diagnostic applications. Despite the complex composition of RPA (6 to 7 proteins in the same reaction mixture), the latter was shown to be rapid (generating results in < 20 min), specific and sensitive (detecting few target genome copies), and applied widely in different fields. Based on these advantages, we assume that RPA has a flexibility allowing it to be used for the rapid diagnosis of infectious diseases thus reducing time-to-result to less than an hour. Consequently, it will be possible to integrate RPA in microfluidic platforms providing a lab-on chip system. The first part of this doctoral project generated additional guidelines for RPA primers/probes design to develop specific RPA diagnostic assays. Second, we developed an RPA diagnostic test for the detection of group B streptococci, responsible for sepsis and meningitis in newborns. This assay was the first to evaluate RPA with human clinical samples. This diagnostic test was compared to a reference method, the polymerase chain reaction (PCR). This demonstration with clinical samples served to carry out the final objective of this project that was to automate RPA in a miniaturized microfluidic centripetal system. Collaboration with engineers and experts in materials has generated the microfluidic device called "blade" and the instrument involved in the operation of various mechanistic tasks. These preliminary results suggested that it will be important to provide an automated system applicable at bedside. Consequently, it will be possible to perform a complete analysis of infectious agents in less than an hour without the need for complex procedures for the preparation and transport of clinical specimens or the assistance of qualified personnel.
Godow, Bratt Tora, Mathilda Stigenberg, Andreas Elenborg, Sarah Ågren, and Andreas Medhage. "To monitor the microbial biodiversity in soil within Uppsala." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-444210.
Повний текст джерелаShahin, Khalid Elsayed Kamal Elsayed. "Development of control strategies for Francisella noatunensis subsp. orientalis in Nile tilapia, Oreochromis niloticus." Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28046.
Повний текст джерелаMoreover, this study has proven the efficacy of a cross protective Fno injection vaccine in tilapia fingerlings, with further optimisation needed for immersion vaccination of fry, and given insights into the immune response of tilapia to vaccination against francisellosis. In addition, it provided a rapid, sensitive, specific and robust molecular tool for detection of Fno that can assist surveillance and control of piscine francisellosis on tilapia farms.
Priyanka, V. "Droplet Isothermal Amplification For Nucleic Acid Quantification." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5643.
Повний текст джерелаChou, Yu-Pao, and 周育葆. "Primer Design for Multiplex Polymerase Chain Reaction and Multiplex Isothermal Recombinase Polymerase Amplification." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/65p49j.
Повний текст джерела國立交通大學
生物資訊及系統生物研究所
106
At present, most of the deoxyribonucleic acid (DNA) amplification techniques such as polymerase chain reaction (PCR). PCR relies on the thermal cycle machine, through denaturation, annealing, extension, the process requires precise control the temperature. Recombinase polymerase amplification (RPA) technology is developed by TwistDx in 2006. First, it makes the primer sequence and protein form a complex, the complex will find the location of the homologous sequence on the DNA template and open double-stranded DNA helix structure. Next, amplification was performed by recombinase polymerase. The temperature of the whole process is maintained at about 37 to 42°C, which will allow the DNA amplification technology get a new breakthrough. DNA amplification technology at constant temperature will no longer need to rely on the thermal cycle machine, enhances this DNA amplification technology’s portability and convenience. However, the most important part of the technology is how to design primers to make the RPA correctly amplify the target gene or sequence. In addition, design primers for multiplex PCR or RPA, it needs to avoid the two primers because the sequence with excessive similarity leads to form primer dimers so that reduce the amplification efficiency. So far, it is still not found that someone provides a primer design for multiplex RPA platform. In this study, we collect RPA primers from literature, and statistics out RPA primer features and integrate the recommendations of primer design from literature. Next, according to as above, use a series of bioinformatics methods like we use Primer3 to generate candidate primer groups, and then we use Bowtie to confirm the specificity of each primer pairs. Finally, the genetic algorithm was used to find out optimized primer group that the temperature between the two primers will not be too high to form primer dimers. In this study, we respectively designed primer sets for multiplex PCR and multiplex RPA to provide future experimental verification, such as gel electrophoresis, next-generation sequencing or Nanopore MinION sequencing platform. In summary, this study develops a web platform and a standalone tool allows users to design multiplex PCR or RPA primer sets that meet their own experimental needs.
Euler, Anna Milena. "Entwicklung von Rekombinase-Polymerase-Amplifikations-Verfahren zum schnellen Nachweis von hochpathogenen Erregern." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0022-603B-0.
Повний текст джерелаEhnts, Kai Ilmo. "Entwicklung von Rekombinase-Polymerase-Amplifikations-Nachweisverfahren für virale Erreger von Atemwegsinfektionen." Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BAD4-F.
Повний текст джерелаFechner, Kim. "Distribution of Mycobacterium avium subspecies paratuberculosis in clinically asymptomatic bulls and different non-ruminant species." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3EF4-9.
Повний текст джерелаЧастини книг з теми "Droplet Recombinase Polymerase Amplification"
Bhat, Alangar Ishwara, and Govind Pratap Rao. "Recombinase Polymerase Amplification." In Springer Protocols Handbooks, 383–87. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0334-5_40.
Повний текст джерелаVashi, Yoya, and Sachin Kumar. "Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases." In Springer Protocols Handbooks, 239–50. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2043-4_17.
Повний текст джерелаGlais, Laurent, and Emmanuel Jacquot. "Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays." In Plant Pathology, 207–25. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2620-6_16.
Повний текст джерелаZhang, Yuhang, Jinqiang Hu, Qingmei Li, Junqing Guo, and Gaiping Zhang. "Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticks." In Methods in Microbiology, 319–49. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mim.2019.11.008.
Повний текст джерелаТези доповідей конференцій з теми "Droplet Recombinase Polymerase Amplification"
Timkin, P. D., and A. A. Penzin. "An experimental approach for diagnosing cercosporosis using RPA+CRISPR/Cas12a." In II All-Russian (national) scientific conference with international participation "Russian Science, Innovation, Education". Krasnoyarsk Science and Technology City Hall, 2023. http://dx.doi.org/10.47813/rosnio-ii.2023.8.263-266.
Повний текст джерелаSirr, Noel, Doina Ciobanu, Ronan Grimes, and Mark Davies. "A Continuous Flow Polymerase Chain Reactor for DNA Expression Analysis." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96180.
Повний текст джерелаGaiani, Greta, Mònica Campàs, Anna Toldrà, Maria Rey, Karl Andree, Carles Alcaraz, Jorge Diogène, and Ciara O'Sullivan. "Recombinase Polymerase Amplification for <em>Gambierdiscus </em>and <em>Fukuyoa </em>detection: a step further in the ciguatera risk management." In 1st International Electronic Conference on Toxins. Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/iect2021-09168.
Повний текст джерелаSayers, Michael B., and Tara M. Dalton. "A Novel Contamination Free Two Temperature Continuous Flow Polymerase Chain Reactor." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43055.
Повний текст джерелаBarrett, Brian, Mark Davies, and Angela Morris. "Thermal Analysis of a Novel Continuous Flow Multi Layered Polymerase Chain Reaction Device." In ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference collocated with the ASME 2007 InterPACK Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ht2007-32792.
Повний текст джерела