Дисертації з теми "Direct nucleic acid detection"
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Lores, Lareo Pablo. "Nucleic acids and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/20133.
Повний текст джерелаThe field of nucleic acid detection has evolved swiftly in recent years. From quantification of micro RNA for the study of cell death, proliferation, and regulation, to the assessment of the influence of genetic variability towards disease development and treatment, the analysis of nucleic acids will play a central role in future medicine. In that regard, the detection of SNPs, as the primary source of genetic variability and the most challenging mutation from the analytical point of view, will be at the forefront of the discussion. Methods for the detection of SNPs not only require sensitivity, selectivity and robustness, but they should also allow multiplexing and offer high throughput in order to face the growing analysis demand In this work an assay for the detection of nucleic acids and single nucleotide polymorphisms (SNPs) was developed. The reaction system for the detection of nucleic acids is based on the interaction between two modified peptide nucleic acid (PNA) oligonucleotides. The first incorporated a C-terminal thioester (donor probe), and the second one a N-terminal cysteinyl residue (acceptor probe). In addition, the donor probe is functionalized with a metal-tag, which consist of a macrocyclic metal chelate complex of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) with a chelated lanthanoide. A biotin tag for purification by streptavidin magnetic particles was incorporated in the acceptor probe. The target DNA strand brings together the reporter probes allowing the chemical reaction. The resulting ligation product contains the metal-tag and the biotin, which is used to purify the product before measurement in the ICP-MS system. The lanthanoid concentration is used as an indicator of the ligation product, which at the same time serves as reporter of the target template. The methodological limit of detection achieved with this system was 29 pM with RSD of 6.8% at 50 pM (n=5). Detection of SNPs was performed using a combination of two sets of PNA probes labeled with different lanthanoid metal tags. The first probe set targeted the sequence where the SNP was present (reporter probe system), while the second set of probes was designed to bind to a neighboring sequence (control probe system). The signals of both lanthanides were used to establish a ratio that allowed the detection of the SNP. This assay was successfully used to simultaneously differentiate between alleles of 3 SNPs by measuring six lanthanoids at 5 nM concentration.
Chatwell, Nicola. "Nucleic acid approaches to toxin detection." Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606582.
Повний текст джерелаBehrmann, Ole [Verfasser], and Gerald A. [Akademischer Betreuer] Urban. "Methods for rapid nucleic acid extraction and detection." Freiburg : Universität, 2021. http://d-nb.info/1227187289/34.
Повний текст джерелаFerrier, David Christopher. "Nucleic acid detection using oligonucleotide cross-linked polymer composites." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28944.
Повний текст джерелаGorgannezhad, Lena. "Advanced Technologies in Rapid and Multiplex Detection of Nucleic acid." Thesis, Griffith University, 2020. http://hdl.handle.net/10072/397045.
Повний текст джерелаThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
Full Text
Kershaw, David Michael. "Nanoparticle bound nucleic acid probes for DNA detection and gene inactivation." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7432/.
Повний текст джерелаSaeed, Ibrahim Q. "Optoelectronically active sensitisers for the selective detection of nucleic acid biomarkers." Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/100885/.
Повний текст джерелаO'Meara, Deirdre. "Molecular Tools for Nucleic Acid Analysis." Doctoral thesis, Stockholm : Tekniska högsk, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3220.
Повний текст джерелаKhater, Mohga Wagdy Yehia Mohamed. "Nanoparticle-based sensors for pathogen nucleic acid detection with interest for agriculture." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667373.
Повний текст джерелаThis thesis aims at developing sensitive, affordable and portable biosensors based on nanomaterials for the determination of nucleic acid related to plant pathogens. The work strives to contribute to the keeping up in the advancements of biosensing systems relevant to plant infection diagnostics which would be an essential solution in the future to the issues of plant disease monitoring and food security. Following Chapter I, state-of-the-art on the latest trends in the development of advantageous biosensors based on both antibody and DNA receptors for early plant disease detection, as well as the use of different nanomaterials such as nanochannels and metallic nanoparticles for the development of innovative and sensitive biosensing systems for the detection of pathogens (i.e. bacteria and viruses) at the point-of-care is given. The next sections of this dissertation will describe three diagnostic biosensing strategies for the detection of citrus tristeza virus (CTV) related nucleic acid using electrical and optical transducing techniques. The electrical sensing of CTV through DNA hybridization based approach and the in situ amplified nucleic acid method will be achieved on carbon sensing substrate modified with gold nanoparticles, while paper-based sensors will be operated in lateral flow format for the gold nanoparticle-based optical detection of CTV. Furthermore, all aspects of the developed biosensing systems, from the bioassay and biosensor design to their development and optimization are presented in which will be organized in the following manner: Chapter III will present highly specific DNA hybridization sensor based on AuNP-modified SPCE employing label-free impedance for the detection of the CTV-related nucleic acid, together with dedicating emphasis to the study of electrodeposition time of AuNPs, whose precise particle size and shape will be required for the enhancement of DNA hybridization rate. A set of voltammetric studies of deposited AuNPs will be discussed. Particular attention will be paid for assembling the thiolated DNA probe as sensing layer for biosensor construction. The main sensor design aspects such as AuNPs size, probe DNA concentration and immobilization time together with DNA hybridization time will be optimized, in order to precisely select the best working conditions for this diagnostic platform. Chapter IV will cover the whole process undertaken for preparation of in situ nucleic acid amplification on gold nanoparticle-modified sensor for sensitive and quantitative detection of CTV. Plant disease (Citrus tristeza virus (CTV)) diagnostics was selected as relevant target for the demonstration of the proof-of-concept. This chapter will include two parts, the first one focuses on the design of RPA amplification assay, primers design, optimization of all essential bioassay aspects such as amplification temperature, volume and screening primers and finally the electrophoresis analysis for RPA products. The second part of this chapter will demonstrate label-free highly integrated in situ RPA amplification/detection approach at room temperature that takes advantage of the high sensitivity offered by gold nanoparticle-modified sensing substrates and electrochemical impedance spectroscopic (EIS) detection. Chapter V focuses on the application of isothermal nucleic acid amplification technology in simple lateral flow platform. The preparation of AuNP-based LFA for the highly sensitive direct detection of RPA amplified nucleic acid, the assembling of lateral flow step, the conjugation of AuNPs to the antibodies used for colorimetric detection, as well as the optimization of all working conditions and finally the analytical performance of the bioassay in LF will be explored. Moreover, aiming at truly achieving the point of care requirements of simple and affordable diagnostic technologies, the work here will present the possibility of amplifying nucleic acid without heat source and visual color detection. This approach would be of great potential as point of care diagnostics.
Baloda, Meenu. "Lateral Flow Nucleic Acid Biosensor for the Detection of Sexually Transmitted Diseases." Diss., North Dakota State University, 2015. https://hdl.handle.net/10365/27596.
Повний текст джерелаStains, Cliff. "Methods for the Detection of Protein-Nucleic Acid and Protein-Protein Interactions." Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/194834.
Повний текст джерелаMokany, Elisa Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "The development of multi-component nucleic acid enzymes(MNAzymes)for the detection of analytes." Awarded by:University of New South Wales, 2007. http://handle.unsw.edu.au/1959.4/35210.
Повний текст джерелаBadran, Ahmed Hussein. "Split-Protein Systems for the Detection and Interrogation of Protein-Nucleic Acid Interactions." Thesis, The University of Arizona, 2010. http://hdl.handle.net/10150/146847.
Повний текст джерелаThomas, Alistair Owen. "Detection of bacterial gene expression by a novel isothermic nucleic acid amplification technology." Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410924.
Повний текст джерелаGorbunova, Santa Maria. "Electrochemical characterization of carminic acid towards the use as an electrochemical molecular beacon for nucleic acid detection." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52894.
Повний текст джерелаScience, Faculty of
Chemistry, Department of
Graduate
Periyannan, Rajeswari Prem Kumar. "Droplet microfluidics for single cell and nucleic acid analysis." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-192668.
Повний текст джерелаQC 20160926
Zozulia, Oleksii [Verfasser], and Andriy [Gutachter] Mokhir. "Red light-triggered nucleic acid-templated reactions for detection of nucleic acids in live cells / Oleksii Zozulia ; Gutachter: Andriy Mokhir." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2017. http://d-nb.info/1135779791/34.
Повний текст джерелаTomlinson, Jennifer A. "Nucleic acid-based methods for on-site detection of plant pathogens : approaches and applications." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12957/.
Повний текст джерелаXiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.
Повний текст джерелаDerbyshire, Nicola. "Exploring the application of nucleic acid aptamers for detection of food contaminants and viruses." Thesis, University of Leeds, 2012. http://etheses.whiterose.ac.uk/4136/.
Повний текст джерелаHernández-Neuta, Iván. "Nucleic acid analysis tools : Novel technologies and biomedical applications." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-146334.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 1: Manuscript.
Metcalf, Gavin Alan Dorman. "Fluorogenic Peptide Nucleic Acid probes for the detection of circulating microRNAs : applications to cancer diagnosis." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/45000.
Повний текст джерелаTsourkas, Andrew. "Development and optimization of dual FRET-molecular beacons for the detection and visualization of single-stranded nucleic acid targets." Diss., Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/19256.
Повний текст джерелаSpencer, Sarah M. "Development of RNA Microchip for the Detection of Pathogens." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/chemistry_diss/35.
Повний текст джерелаGrant, Paul Robert. "Development and application of nucleic acid amplification technology (NAT) for the detection of viruses in donated blood." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408734.
Повний текст джерелаRichardson, Kenneth James. "Use of nucleic acid probes and a nonradioactive labeling system for the detection of enteroviruses in water." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184948.
Повний текст джерелаSailis, Fiammetta. "Detection of miRNA by SMART technology." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28891.
Повний текст джерелаKerr, Samantha Louise. "Enhancing nucleic acid detection using inductively coupled plasma mass spectrometry, by means of metal and nano-particle labelling." Thesis, Loughborough University, 2008. https://dspace.lboro.ac.uk/2134/4641.
Повний текст джерелаFredriksson, Simon. "Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders." Doctoral thesis, Uppsala University, Department of Genetics and Pathology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2691.
Повний текст джерелаA novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. As pairs of so-called proximity probes bind the individual target protein molecules at distinct sites, these reagents are brought in close proximity. The probes consist of a protein specific binding part coupled to an oligonucleotide with either a free 3’- or 5’-end capable of hybridizing to a common connector oligonucleotide. When the probes are in proximity, promoted by target binding, then the DNA strands can be joined by enzymatic ligation. The nucleic acid sequence that is formed can then be amplified and quantitatively detected in a real-time monitored polymerase chain reaction. This convenient assay is simple to perform and allows highly sensitive protein detection. Parallel analysis of multiple proteins by DNA microarray technology is anticipated for proximity ligation and enabled by the information carrying ability of nucleic acids to define the individual proteins. Assays detecting cytokines using SELEX aptamers or antibodies, monoclonal and polyclonal, are presented in the thesis.
Microarrays synthesized in situ using photolithographic methods generate impure products due to damaged molecules and interrupted synthesis. Through a molecular inversion mechanism presented here, these impurities may be removed. At the end of synthesis, full-length oligonucleotides receive a functional group that can then be made to react with the solid support forming an arched structure. The 3’-ends of the oligonucleotides are then cleaved, removing the impurities from the support and allowing the liberated 3’-hydroxyl to prime polymerase extension reactions from the inverted oligonucleotides. The effect of having pure oligonucleotides probes compared to ones contaminated with shorter variants was investigated in allele specific hybridization reactions. Pure probes were shown to have greater ability to discriminate between matched and singly mismatched targets at optimal hybridization temperatures.
Zhang, Zhiling. "The structure and function of troponin T upon metal ion binding and the detection of nucleic acid sequence variations." Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc5565/.
Повний текст джерелаTang, Yeuk-nam Kennie. "A comparison of DIG nonradioactive with 32p radioactive nucleic acid labeling of Southern blot for the detection of alpha thalassaemia /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B32037661.
Повний текст джерелаTang, Yeuk-nam Kennie, and 鄧若楠. "A comparison of DIG nonradioactive with 32p radioactive nucleic acid labeling of Southern blot for the detection of alpha thalassaemia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B45010456.
Повний текст джерелаSilverman, Adam Phillip. "Part I: Detection of RNA in cells with quenched autoligation probes ; probing active site tightness in nucleic acid replication enzymes /." May be available electronically:, 2007. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Повний текст джерелаBernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.
Повний текст джерелаNicolini, Ariana Marie, and Ariana Marie Nicolini. "Single-Step, Optical Biosensors for the Rapid and Sensitive Detection of Bacterial and Viral Pathogens." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/623158.
Повний текст джерелаFun, Sze-tat. "Development of molecular diagnostic system for detection of hepatitis B virus in blood donations." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971751.
Повний текст джерелаPinto, Alessandro. "Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/80744.
Повний текст джерелаNicholson, Wendy Jane. "The application of the polymerase chain reaction to the detection and characterization of human immunodeficiency virus and hepatitis B virus nucleic acid." Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/21446.
Повний текст джерелаMcCauley, Sean Matthew. "Innate Detection of HIV-1 in Myeloid Dendritic Cells." eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/993.
Повний текст джерелаWeldhagen, Gerhard Frederick. "Laboratory Detection and Gene Cassette Stability of the Novel Extended-Spectrum Beta-Lactamase, GES-2 from Pseudomonas aeruginosa." Thesis, University of Pretoria, 2004. http://hdl.handle.net/2263/29221.
Повний текст джерелаThesis (PhD (Medical Microbiology))--University of Pretoria, 2006.
Medical Microbiology
unrestricted
Porter, Jason Robert. "SPLIT-PROTEIN REASSEMBLY METHODS FOR THE DETECTION AND INTERROGATION OF BIOMOLECULAR INTERACTIONS AND MODULATORS THEREOF." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/194359.
Повний текст джерелаLehnus, Massimiliano. "Bio-BCA (Bio-Barcode Cascade Amplification) : development of a photosensitive, DNA-based exponential amplification platform technology for the detection of nucleic acid biomarkers." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277915.
Повний текст джерелаSeitz, Oliver [Gutachter], Michael W. [Gutachter] Linscheid, and Ilko [Gutachter] Bald. "Nucleic acids and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry / Gutachter: Oliver Seitz, Michael W. Linscheid, Ilko Bald." Berlin : Humboldt-Universität zu Berlin, 2019. http://d-nb.info/119993061X/34.
Повний текст джерелаMantha, Stacey. "Direct detection and rapid identification of clinically significant mycobacteria from Bactec 12B medium and acid-fast bacilli positive sputum specimens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0015/MQ54472.pdf.
Повний текст джерелаLam, Yiu-pong, and 林耀邦. "Performance evaluation of the automated NucliSens easyMAG and Qiagen EZ1 Advanced XL nucleic acid extraction platform for detection of RNAand DNA viruses in clinical samples." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46448020.
Повний текст джерелаFun, Sze-tat, and 范思達. "Development of molecular diagnostic system for detection of hepatitis B virus in blood donations." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971751.
Повний текст джерелаFaltin, Bernd [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Mediator Probe PCR: a novel assay principle for universal real-time detection of nucleic acid amplification = Mediator Probe PCR: ein neuartiger Ansatz zur universellen Echtzeit-Detektion von Nukleinsäuren." Freiburg : Universität, 2013. http://d-nb.info/1123477000/34.
Повний текст джерелаCeylan, Koydemir Hatice. "Mems Based Electrochemical Dna Sensor To Detect Methicillin Resistant Staphylococcus Aureus And Vancomycin Resistant Enterococcus Species." Phd thesis, METU, 2013. http://etd.lib.metu.edu.tr/upload/12615402/index.pdf.
Повний текст джерелаECS) to detect the methicillin resistance in Staphylococcus aureus and vancomycin resistance in Enterococcus species. To the best of our knowledge, the developed sensor is the first µ
ECS which utilizes on-chip reference (Ag), working (Au), and counter (Pt) electrodes together with a microchannel to detect MRSA and VRE. The characterization of the designed sensor was achieved analyzing the interactions of the buffer solutions and solvents with the electrodes and Parylene C film layer by using optical and electrochemical methods. Specific parts of genes that are indicators of antimicrobial resistances were used in order to detect the resistances with high selectivity and sensitivity. Thus, synthetic DNA and bacterial PCR product were used as target probes in redox marker based detection and enzyme based detection, respectively. In order to enhance the hybridization, folding structures of the capture probe were investigated by using mfold Web Server. In redox marker based detection, the hybridization of DNA was indirectly detected by using Hoechst 33258 as redox marker with differential pulse voltammetry. The cross reactivity of the tests were performed by using different target probes of femA genes of S. aureus and S. epidermis, which are the major genes detected in methicillin detection assays. Consequently, amplification of signal by using horseradish peroxidase and TMB/H2O2 as substrate was achieved in order to enhance detection sensitivity. The sensor could detect 0.01 nM 23-mer specific part of mecA gene with redox marker based detection and 10 times diluted PCR product with enzyme-based detection in about six hours including the steps of sample preparation from whole blood. This sensor with its compatibility to MEMS fabrication processes and IC technology has a promising potential for a hand-held device for POC through the integration of micropotentiostat.
VERMA, SUJIT KUMAR [Verfasser], Sebastian [Akademischer Betreuer] Springer, Sebastian [Gutachter] Springer, Michael [Gutachter] Köhler, Mathias [Gutachter] Winterhalter, and Werner M. [Gutachter] Nau. "Polyelectrolyte Microcapsules: A versatile and sensitive tool for the detection of protein and nucleic-acid analytes / SUJIT KUMAR VERMA ; Gutachter: SEBASTIAN SPRINGER, MICHAEL KÖHLER, MATHIAS WINTERHALTER, WERNER M. NAU ; Betreuer: SEBASTIAN SPRINGER." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2017. http://d-nb.info/114810397X/34.
Повний текст джерелаFischbach, Melanie. "Haarnadelförmige PNA-Peptid-Konjugate." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17305.
Повний текст джерелаThe developmental stage of certain diseases is closely linked to the concentration of various proteins in biological samples. A sensitive detection of these so-called biomarkers can thus significantly contribute to an early diagnosis. In the present work, structured, fluorogenic probes were developed that offer the possibility of a sensitive, direct and in real-time detection of target proteins in a homogeneous process. The peptidic recognition sequence for the target protein was thereby flanked by two self-complementary PNA segments. As a result, the probes possessed a hairpin-type arrangement, in which suitable appended labels are forced into close proximity and guaranteed a minimal background signal in the unbound state. By interacting with the target protein a reorganization of the probe structure occured, which could be followed by fluorescence spectroscopy. To embed the fluorogenic units different approaches were developed and the resulting architectures were validated relating to their use as a sensitive detection system. As target proteins the intracellular SH2-domains of the Src and Lck kinase and the extracellular matrix metalloprotease MMP-7, a proteolytic biomarker for cancer, were investigated. In particular, the new In-Stem Hairpin Peptide Beacons (IS-HPBs), in which fluorogenic pseudo nucleic acids were incorporated into the PNA-stem region, proved as sensitive protease reporters with an up to 50-fold signal amplification. By using an excimer-signaling IS-HPB and a time-resolved fluorescence method the direct detection of MMP-7 with a critical concentration of 1 nM within complex human blood serum was achieved. A possible application in medical diagnostics was thus confirmed. Furthermore, initial indications were obtained using thermodynamic studies that the structure of a peptide-based probe contributes to increased selectivity.