Дисертації з теми "Diabetes biomarkers"
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Wu, Di. "Discovery of glyco-biomarkers for diabetes and complications in diabetes." Thesis, University of Dundee, 2015. https://discovery.dundee.ac.uk/en/studentTheses/e2eee4c8-c74c-4fc6-8658-ab51ecd793de.
Повний текст джерелаDias, Stephanie Charmaine. "Investigating Molecular Biomarkers During Gestational Diabetes Mellitus." Thesis, University of Pretoria, 2019. http://hdl.handle.net/2263/73566.
Повний текст джерелаThesis (PhD)--University of Pretoria, 2019.
National Research Foundation (NRF) of South Africa, Thuthuka Grant (unique grant no. 99391).
South African Medical Research Council (SAMRC)
Obstetrics and Gynaecology
PhD
Unrestricted
Wang, Yudong, and 汪玉東. "Neutrophil serine proteases as novel biomarkers for autoimmune diabetes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208026.
Повний текст джерелаpublished_or_final_version
Medicine
Doctoral
Doctor of Philosophy
Azmi, Shazli. "Longitudinal studies in metabolic neuropathies : development of imaging biomarkers." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/longitudinal-studies-in-metabolic-neuropathies-development-of-imaging-biomarkers(6913e957-0e81-4af8-a544-f943f0105b8c).html.
Повний текст джерелаAnthony, Yancke. "Identification and validation of micrornas for diagnosing type 2 diabetes : an in silico and molecular approach." University of the Western Cape, 2015. http://hdl.handle.net/11394/4713.
Повний текст джерелаType 2 diabetes mellitus (T2DM), a metabolic disease characterized by chronic hyperglycemia, is the most prevalent form of diabetes globally, affecting approximately 95 % of the total number of people with diabetes i.e. approximately 366 million. Furthermore, it is also the most prevalent form in South Africa (SA), affecting approximately 3.5 million individuals. This disease and its adverse complications can be delayed or prevented if detected early. Standardized diagnostic tests for T2DM have a few limitations which include the inability to predict the future risk of normal glucose tolerance individuals developing T2DM, they are dependent on blood glucose concentration, its invasiveness, and they cannot specify between T1DM and T2DM. Therefore, there is a need for biomarkers which could be used as a tool for the early and specific detection of T2DM. MicroRNAs are small non-coding RNA molecules which play a key role in controlling gene expression and certain biological processes. Studies show that dysregulation of microRNAs may lead to various diseases including T2DM, and thus, may be useful biomarkers for disease detection. Therefore, identifying biomarkers like microRNAs as a tool for the early and specific detection of T2DM, have great potential for diagnostic purposes. The main focus of this investigation, therefore, is the early detection of T2DM by the identification and validation of novel biomarkers. Furthermore, based on previous studies, the aim of the investigation was to identify differentially expressed miRNAs as well as identify their potential target genes associated with the onset and progression of T2DM. An in silico approach was used to identify miRNAs found to be differentially expressed in the serum/plasma of T2DM individuals. Three publically available target prediction software were used for target gene prediction of the identified miRNA. The target genes were subjected to functional analysis using a web-based software, namely DAVID. Functions which were clustered with an enrichment score > 1.3 were considered significant. The ranked target genes mostly had gene ontologies linked with “transcription regulation”, “neuron signalling, and “metal ion binding”. The ranked target genes were then split into two lists – an up-regulated (ur) miRNA targeted gene list and a down-regulated (dr) miRNA targeted gene list. The in silico method used in this investigation produced a final total of 4 miRNAs: miR-dr-1, miR-ur-1, miR-ur-2, and miR-ur-3. Based on the bioinformatics results, miR-dr-1 and its target genes LDLR, PPARA and CAMTA1, seemed the most promising miRNA for biomarker validation, due to the function of the target genes being associated with T2DM onset and progression. The expression levels of the miRNAs were then profiled in kidney tissue of male Wistar rats that were on a high fat diet (HFD), streptozotocin (STZ)-induced T1DM, and non-diabetic control rats via qRT-PCR analysis. The hypothesis was that similar miRNA expression would be found in the HFD kidney samples compared to serum expression levels of the miRNA obtained from the two databases, since kidneys are involved in cleansing the blood from impurities. This hypothesis proved to be true for all miRNAs except for miR-ur-2. Additionally, miR-ur-1 seemed the most significant miRNA due to it having different expression ratios for T1DM and T2DM (i.e. -7.65 and 4.2 fold, respectively). Future work, therefore, include validation of the predicted target genes to the miRNAs of interest i.e. miR-dr-1: PPARA and LDLR and miR-ur-1: CACNB2, using molecular approaches such as the luciferase assays and western blots.
McDonald, Timothy James. "Identification of monogenic diabetes utilising biomarkers clinical criteria, and molecular genetics." Thesis, Exeter and Plymouth Peninsula Medical School, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553697.
Повний текст джерелаPrice, Anna Helen. "Biomarkers for cardiovascular risk prediction in people with type 2 diabetes." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28785.
Повний текст джерелаManeerattanasuporn, Tiwaporn. "Maternal Diabetes, Related Biomarkers and Genes, and Risk of Orofacial Clefts." DigitalCommons@USU, 2017. https://digitalcommons.usu.edu/etd/6215.
Повний текст джерелаWotherspoon, Amy Christine. "Biomarkers and outcomes of the diabetes and pre-eclampsia intervention trial (DAPIT)." Thesis, Queen's University Belfast, 2017. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.728831.
Повний текст джерелаNorberg, Margareta. "Identifying risk of type 2 diabetes : epidemiologic perspectives from biomarkers to lifestyle." Doctoral thesis, Umeå : Umeå universitet, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-953.
Повний текст джерелаSukumar, Nithya. "Novel biomarkers associated with gestational diabetes mellitus and metabolic outcomes of pregnancy." Thesis, University of Warwick, 2017. http://wrap.warwick.ac.uk/90895/.
Повний текст джерелаNowak, Christoph. "Insulin Resistance : Causes, biomarkers and consequences." Doctoral thesis, Uppsala universitet, Molekylär epidemiologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-316891.
Повний текст джерелаGe, Siqi. "Screening of multi-omics biomarkers for Type 2 Diabetes Mellitus among Chinese population." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2019. https://ro.ecu.edu.au/theses/2293.
Повний текст джерелаSharma, Masti Venugopal Srihari. "Identification of diagnostic biomarkers to improve the management of diabetes-related foot ulcers." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/116374/1/Masti%20Venugopal%20Srihari_Sharma_Thesis.pdf.
Повний текст джерелаDonovan, Brittney Marie. "Early risk prediction tools for gestational diabetes mellitus." Diss., University of Iowa, 2018. https://ir.uiowa.edu/etd/6408.
Повний текст джерелаRobertson, Christine Mary. "Cardiovascular disease, type 2 diabetes and carotid ultrasound." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/21087.
Повний текст джерелаCosta, Priscila Paganini. "Biomarcadores salivares de pacientes periodontais com diabetes mellitus tipo 2." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-30062008-154755/.
Повний текст джерелаBackground: Association of diabetes and periodontitis produces an inflammatory proteins discharge that can be reflected in saliva. The aim of this study was to measure salivary concentrations of interleukin-6 (IL-6), matrix metalloproteinase-8 (MMP-8) and osteoprotegerin (OPG) in patients with chronic periodontitis associated or not to type 2 diabetes mellitus. Methods: A total of 90 subjects was divided in four groups: healthy (Control, n=22), patients with Periodontal Disease (PD, n=24), Diabetes Mellitus only (DM, n=20), Periodontal Disease and Diabetes Mellitus (PD+DM, n=24). Clinical and metabolic data were recorded. Non-stimulated saliva samples were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: Significant differences were detected between groups for the biomarkers (p<0.05). Regarding IL-6, significant differences were found comparing Control with PD and with PD+DM, and comparing PD+DM with DM groups (p<0.005). For MMP- 8, the concentrations mean of the Control group was significantly lower than all the diseased groups (p<0.01), and no differences between diseased groups were detected. For OPG, significant differences were found between Control and PD, and between Control and DM groups (p<0.05). All clinical parameters were significant between groups (p<0.001), except suppuration. In PD group, BOP (bleeding on probing) showed positive correlation with IL-6 concentrations (r=0.48; p<0.05), PPD>=7 (pocket depth>=7mm) correlated positively with MMP-8 concentrations (r=0.46; p<0.05), also HbA1c levels correlated positively with IL-6 concentrations (r=0.54; p<0.000). Conclusion: Saliva is an adequate substrate for inflammatory biomarkers identification in periodontal patients. IL-6 is a candidate biomarker for periodontitis and periodontitis associated to diabetes in the saliva.
Costa, Priscila Paganini. "Biomarcadores diagnósticos relacionados à atividade da doença periodontal em diabéticos." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-23052012-161622/.
Повний текст джерелаThe overall aim of this study was to monitor the periodontal disease activity and suggest potential salivary biomarkers related to this activity in chronic periodontitis patients with or without type 2 Diabetes mellitus (DM), based on the evaluation of gene expression profile of progressive periodontal sites and salivary inflammatory proteins. Fifty-six patients were enrolled, 21 with chronic periodontitis (PD group), 20 with chronic periodontitis and DM (PD+DM group) and 15 periodontal- and systemically healthy (control). Radiographs were taken before and two months after non-surgical periodontal therapy, and radiographic subtraction was performed from pairs of these radiographs. Measurements of the areas with density loss were recorded. Unstimulated saliva collection, glycated hemoglobin (HbA1c) measurement and periodontal examination probing pocket depth (PPD), relative clinical attachment level (rCAL), bleeding on probing (BOP) and plaque index (PI) were also conducted before and two months after non-surgical periodontal therapy. The periodontal sites with progressive attachment loss 1 mm at the recall visit were considered active sites according to the adapted method of tolerance. Gingival biopsies of active and non-active sites with similar clinical parameters were harvested for gene expression analysis of the immune-inflammatory response with Real Time PCR Array. Saliva samples were analyzed by Multiplex Cytokine Profiling Immunoassay for analysis of protein expression profile. In PD group, 9% of the sites were classified as active and in PD+DM group, 12% (p > 0.05). The clinical attachment loss mean was higher in the PD+DM group (1.34±0.23 mm) compared to the PD group (1.21±0.16 mm) (p < 0.05). There was a correlation between clinical attachment loss and darkened radiographic areas in active sites of the PD group (R = 0.79, p = 0.001) and PD+DM group (R = 0.86, p < 0.001). Both PD and PD+DM groups showed a down-regulated profile compared to healthy subjects (control group). When compared PD group to PD+DM, patients with diabetes had an upregulated profile. Active sites of the PD group showed nine genes (ABCF1, CD40LG, IL10, IL5, CCR2, CCR4, CCR7, CCL18 and CXCL1) differentially expressed (p < 0.05) with an up-regulated profile. Active sites of the PD+DM group showed six genes (LTA, CXCR1, CCL19, CCL8, CCL17 and CXCL12) differentially expressed (p < 0.05) with an up-regulated profile. After non-surgical periodontal therapy, there was a significant reduction of clinical parameters and HbA1c levels (p < 0.05), accompanied by a reduction of some salivary proteins (IL1b, IL1ra, IL10, IL17, TGFb, IL8, eotaxin and MCP-3) in groups PD and PD+DM, but without statistically significant difference (p > 0.05). In conclusion, this study was able to monitor the periodontal disease activity in periodontal patients with or without diabetes after the non-surgical periodontal therapy; it was possible to identify genes differentially expressed in active sites from both groups, which may be considered useful in indicating potential biomarkers for the diagnosis of active periodontal disease; salivary proteins show a trend in distinguishing the standard of health and disease and may be used in the future as potential biomarkers of periodontitis with or without diabetes.
Sharma, G. "Methods for the measurement of urinary biomarkers of oxidative stress application to type 1 diabetes mellitus." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1344091/.
Повний текст джерелаBarbosa, Sara Castanho. "Avaliação da função renal em cães diabéticos : análise de indicadores." Master's thesis, Universidade de Lisboa, Faculdade de Medicina Veterinárias, 2020. http://hdl.handle.net/10400.5/19846.
Повний текст джерелаA diabetes mellitus (DM) é a doença mais comum do pâncreas endócrino no cão. É uma síndrome caracterizada por hiperglicemia crónica, glicosúria, polifagia, poliúria/polidipsia e perda de peso. A nefropatia diabética (ND) corresponde a uma possível complicação da DM que, no caso dos humanos, corresponde à principal causa de doença renal crónica nos países ocidentais. A ND é caracterizada por microalbuminúria, proteinúria, hipertensão arterial sistémica e perda de função renal. Os estudos realizados no âmbito da ND canina são escassos e, apesar da sua existência estar documentada, a sua expressão na prática clínica é baixa, o que a torna um tópico pouco discutido. Este estudo teve como principal objetivo avaliar a função renal em cães com diagnóstico de DM, assim como a ocorrência de proteinúria, de modo a identificar a presença, ou não, de ND. Foi também avaliada a correlação entre estas variáveis e o tempo desde o diagnóstico de DM e a dose de insulina instituída. O estudo incluiu 18 cães diabéticos e 17 cães saudáveis de idades e pesos idênticos. Nos cães diabéticos, 38,9% apresentaram um RPCU superior a 0,5. Este grupo apresentou ainda uma razão de possibilidades (odds ratio) de 10,87 (95% IC, 1,71-127,08, p <0,05) de desenvolver proteinúria em relação aos saudáveis. Apesar de os níveis de ureia não diferirem significativamente entre os dois grupos, o grupo diabético apresentou concentrações de creatinina e DMAS inferiores às encontradas no grupo saudável (p<0,05). Não foi encontrada uma correlação significativa entre as concentrações de ureia, creatinina, DMAS ou o RPCU e a duração do diagnóstico de DM, ou com a dose de insulina em curso. Apesar de a ND clínica ser improvável em cães, os resultados obtidos evidenciam o possível efeito da DM a nível renal. Os níveis inferiores de creatinina e DMAS nos cães diabéticos sugerem a ocorrência de hiperfiltração glomerular, o que pode indicar a presença de alterações hemodinâmicas renais. Além disto, estes cães apresentam uma maior possibilidade de desenvolver proteinúria, o que reforça a importância da monitorização do RPCU nestes animais. Este é o primeiro estudo de que temos conhecimento que não só avalia os valores de DMAS em cães diabéticos, como também os compara com os encontrados em cães saudáveis. Dos resultados obtidos poderemos inferir que os animais do nosso estudo com DM se possam encontrar numa fase de pré-nefropatia diabética, numa fase silenciosa, ou numa fase de nefropatia diabética incipiente. Continuam a ser necessários mais estudos que elucidem o impacto da DM sobre os biomarcadores de função e lesão renal de modo a melhor esclarecer a sua relevância na prática clínica veterinária.
ABSTRACT - Diabetes mellitus (DM) is the most common disease of the endocrine pancreas in dogs. It’s a syndrome characterized by chronic hyperglycaemia, glycosuria, polyphagia, polyuria/polydipsia and weight loss. Diabetic nephropathy (DN) is a possible complication of DM that, in humans, is the main cause of chronic kidney disease in western countries. DN includes microalbuminuria, proteinuria, systemic hypertension and impaired kidney function. There are only a few studies regarding canine DN and, even though there are some reports of its occurrence in dogs, it isn’t a popular topic because of how rare it is in clinical practice. The aim of this study was to evaluate the renal function of dogs diagnosed with DM, as well as the occurrence of proteinuria, in order to identify the presence or absence of DN. We also tested if there was a correlation between these biomarkers and the time of diagnosis of DM and the insulin dosage they were being treated with. The study included 18 diabetic dogs and 17 healthy dogs with similar ages and weights. 38,9% of the diabetic dogs had an UPC ratio higher than 0,5. This group had an odds ratio of 10,87 (95% IC, 1,71-127,08, p<0,05) of developing proteinuria when compared to healthy ones. Even though the serum urea concentration didn’t differ between the two groups, diabetic dogs had significatively lower serum concentrations of creatinine and SDMA (p<0,05). There was no significant correlation between serum urea, creatinine or SDMA and UPC ratio and the time of diagnosis. We also didn’t find an association between any of these variables and insulin dosage. Even though the occurrence of clinical DN is unlikely in dogs, our results show the possible impact of DM on the kidney. The lower levels of serum creatinine and SDMA seen in diabetic dogs when compared to healthy ones suggest that glomerular hyperfiltration is present, which may be related with hemodynamic changes in the kidney. Besides, these dogs showed a higher chance of developing proteinuria, which reinforces the importance of UPC ratio assessment when monitoring these patients. To our knowledge, this is the first study that not only evaluates SDMA levels in diabetic dogs, but also compares them to those found in healthy ones. Our results suggest that the diabetic dogs in our study can be in a pre-diabetic nephropathy phase, in a silent phase or in an incipient diabetic nephropathy phase. More studies are needed in order to better understand how DM impacts kidney’s function and injury biomarkers and their relevance in veterinary practice.
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Walter, Debra L. "Renal Consequences of Coxsackievirus Infection and Type 1 Diabetes in Non-obese Diabetic Mice." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1526020616767063.
Повний текст джерелаBrowning, Geoffrey Robinson. "Salivary Biomarkers of Acute Stress and Insulin Sensitivity in Nonhuman Primates." Case Western Reserve University School of Graduate Studies / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1354912347.
Повний текст джерелаGutta, Sridevi. "Increased Urinary Angiotensin Converting Enzyme 2 (ACE2) and Neprilysin (NEP) in Type 2 Diabetic Patients." Wright State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1420732616.
Повний текст джерелаFranken, Holger [Verfasser]. "Machine Learning and Computational Mass Spectrometry for the Discovery of Metabolite Biomarkers Involved in Type 2 Diabetes / Holger Franken." München : Verlag Dr. Hut, 2013. http://d-nb.info/1033041831/34.
Повний текст джерелаJuszczak, Agata. "Assessment and translation of novel biomarkers for diagnosis of maturity onset diabetes of the young due to HNF1A variants." Thesis, University of Oxford, 2018. http://ora.ox.ac.uk/objects/uuid:e5dcd952-f092-4269-bb9b-35bfd577a031.
Повний текст джерелаSharpe, Chantelle. "Sex after Gray Hair? Association between Sexual Activity, Hugging, and Health among older Adults?" Scholar Commons, 2017. http://scholarcommons.usf.edu/etd/6951.
Повний текст джерелаEvans, Jonathan. "APOE, PCSK9, and CETP genetic variants as potential biomarkers of dyslipidaemia in black South Africans with Type 2 Diabetes Mellitus." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29630.
Повний текст джерелаJagannathan, Ram. "Identification of biomarkers for type 2 diabetes : analysis of a primary prevention study among Asian Indians with impaired glucose tolerance." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/32118.
Повний текст джерелаAjabshir, Sahar. "The Effect of Vitamin D3 Supplementation of Biomarkers of Oxidative Stress and Glycemic Status in Adults with Type 2 Diabetes." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3649.
Повний текст джерелаSantos, Aritania Sousa. "Expressão de microRNAs circulantes relacionados ao diabetes tipo 1 autoimune." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-14082018-124100/.
Повний текст джерелаINTRODUCTION: Autoimmune type 1 diabetes (T1D) is associated with changes in innate and adaptive immunity. The organ-specific autoimmune aggression determines the destruction of beta-cells in the pancreas and the deficient insulin production. The inflammatory infiltration of the lymphomononuclear type, configuring the insulite, and the scarcity or the absence of the beta cells, define the histological picture of T1D. Autoantibodies against beta-cell antigens, which usually develop in the preclinical phase, confer predisposition to T1D. However, it is difficult to define when and which individuals will progress to overt diabetes, justifying the search for other biomarkers that could be indicative of preventive treatments. In this context, it is known that the microRNAs (miRNAs) - small RNAs that act post transcription - play a crucial role in regulating genes and in integrating genetic and environmental factors, influencing the function of organs and tissues in a punctual or systemic way. OBJECTIVES: to evaluate the biological involvement and relevance of miRNA expression in the immune response and ?-cell function in the pathogenesis of T1D. METHODS: we analyzed the profile of serum miRNAs of 4 groups, namely: patients with T1D up to 6 months after diagnosis (recent T1D), (n = 30); patients with T1D lasting 2-5 years (T1D 2- 5) (n = 26) and individuals expressing pancreatic autoantibodies without diabetes (AbP) (n = 25), which were compared to healthy controls (n = 29). Expression of the microRNAs was obtained with individual assays TaqMan® MicroRNA Assays 5x primers and TaqMan MicroRNA Human Array Card A (Applied Biosystems-Forster City CA, USA), consisting of 377 targets and 4 endogenous. The expression data was analyzed in the Cloud Software (Thermo Fisher Scientific) and Limma (Linear Models for Microarray and RNASeq Data) program. RESULTS: There was no difference in demographic characteristics, such as age, self-reported color, and sex among groups (p > 0.05). Patients with T1D (both recent and 2-5 years), similar to each other, differed from the control group by high glucose, glycated hemoglobin levels, pancreatic autoantibody titers, and lower C peptide values (p < 0.05) . Pancreatic autoantibodies (AbP) carriers had intermediate characteristics among the groups: lower HbA1c and anti-tyrosine phosphatase antibody (anti- IA2) values and higher C-peptide levels than the two groups with diabetes. They differed from controls only by the higher titers of anti-insulin (IAA) and anti-decarboxylase of glutamic acid 65 (anti-GAD65) autoantibodies. The frequency of high risk HLA alleles for diabetes (-DR3 or -DR4 and -DQ2 or DQ- 8) decreased from the recent T1D and T1D 2-5 groups to the AbP and controls. We evaluated 135 miRNAs that were expressed in 20% or more of the samples from the four groups analyzed. Higher expression was observed in 13, 4 and 33 miRNAs of the Abp, recent T1D and T1D 2-5 groups respectively and lower in 11, 7 and 31 miRNAs of these groups. Of these, 4 miRNAs were differentially expressed in the AbP, recent T1D and T1D 2-5 groups in relation to the control group.The miRNAs: miR -16, miR-195 and miR-454, related to endocrine regeneration of the pancreas, anti-inflammatory effect and response to beta-cell injury were decreased in these 3 groups. miR-200a, implicated in beta-cell apoptosis, was increased in the recent and decreased AbP and T1D groups in patients with longer duration of diabetes (T1D 2-5y), possibly due to the shortage of these cells. Another eight miRNAs showed different expression of the control group in two of the evaluated groups, and a similar trend in the third group, four of them high (miR-193a-5p, miR-323-3p, miR-423-5p, and miR- 92a ) and four, decreased (miR-191, miR-19a, miR-376a, miR-590-5p) or neutrality in the 3rd group (miR-15b, miR-100, miR-181a and miR-483-5p) was observed for miR-25 and miR-485-3p, decreased in the AbP group and increased in T1D 2-5y. Such miRNAs are related to immune response, insulin secretion, ?-cell damage and glycotoxicity, similar to that observed for the miR- 101-3p, validated by individual trials in a larger cohort. CONCLUSION Our data suggests that circulating miRNAs may be involved in the pathogenesis of T1D
Ribeiro, Gianine Lima. "EFEITOS DA N-ACETILCISTEÍNA SOBRE O DANO OXIDATIVO RENAL E HEPÁTICO DE RATOS DIABÉTICOS." Universidade Federal de Santa Maria, 2010. http://repositorio.ufsm.br/handle/1/8977.
Повний текст джерелаO diabetes mellitus (DM) é uma doença crônica caracterizada pela hiperglicemia, que está relacionada Com o estresse oxidativo, o qual possui papel importante no desenvolvimento de outras patologias e danos teciduais, tais como dano hepático e renal. Dessa forma, faz-se importante à realização de estudos com possíveis antioxidantes, que possam diminuir os efeitos deletérios do estresse oxidativo decorrentes do diabetes. Neste sentido, a N-acetilcisteína (NAC) é um medicamento utilizado como hepatoprotetor por estimular a síntese de Glutationa Reduzida, diminuindo o dano oxidativo. Nesta linha, o objetivo deste trabalho foi avaliar o efeito antioxidante da NAC nos tecidos renal e hepático de ratos diabéticos através dos biomarcadores do estresse oxidativo como: glutationa reduzida (GSH), glutationa peroxidase (GPx), superóxido dismutase (SOD), malondialdeído (MDA) e ácido delta aminolevulinato desidratase (ALA-D) no fígado e rins de animais controles e com diabetes induzida, tratados e não tratados com NAC. Os tratamentos consistiram em administrações intraperitoneais de 25 mg/Kg e 75 mg/Kg de N-acetilcisteína. No fígado, os níveis de MDA foram significativamente aumentados no grupo diabético comparados ao grupo controle. O tratamento com 75 mg/Kg foi capaz de reduzir os níveis de MDA, ficando semelhantes ao grupo controle. Os níveis da GSH mostrou-se mais elevada no rim e no fígado dos animais diabéticos do que dos controles, e o tratamento com a NAC fez com que esses níveis fossem reduzidos no fígado dos animais diabéticos, entretanto no rim, não houve alterações. Os níveis de SOD e GPx diminuíram no fígado dos animais diabéticos quando comparados ao controle, e a administração de NAC não alterou esses índices. O diabetes também diminuiu a atividade da ALA-D no fígado, e o tratamento com a 25 mg/Kg NAC fez com essa atividade aumentasse significativamente. No tecido renal, ambas as doses de NAC elevaram os níveis de ALA-D nos animais diabéticos. Diante dos resultados encontrados, comparando-se os tecidos renal e hepático dos ratos controles com os diabéticos tratados com NAC, sugere-se que a NAC demonstrou diminuir o dano oxidativo mais no fígado do que no rim.
Čukić, Iva. "Personality traits and health outcomes : an exploration into associations and potential mechanisms." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/16170.
Повний текст джерелаSchamarek, Imke [Verfasser]. "Association between biomarkers of subclinical inflammation and nerve conduction in individuals with recently diagnosed type 1 and type 2 diabetes / Imke Schamarek." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2018. http://d-nb.info/117038899X/34.
Повний текст джерелаPedersen, Henrik Bo. "The effect of time-restricted feeding on glycemic biomarkers : A literature study." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-97732.
Повний текст джерелаMartins, Emerson Renato. "Analise de componentes do metabolismo lipídico na resposta inflamatória induzida por ligadura e punção cecal em ratos diabéticos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/5/5164/tde-15122017-124007/.
Повний текст джерелаINTRODUCTION: Sepsis remains among the leading causes of death in developed and underdeveloped countries with an incidence that increases every year. The increased incidence of sepsis in diabetic patients admitted to intensive care units (ICUs) has also drawn attention due to the complexity of the disease and other metabolic complications, leading to immune deregulation, multiple organ failure and high mortality rates. Lipid metabolism in critically ill diabetic patients has been widely investigated in the last decades, since it is an important characteristic in the pathophysiology of this complex disease. OBJECTIVE: The aim of this study was to investigate the lipid profile of diabetic rats, submitted to cecal ligation and puncture. METHODS: Wistar rats 8-week were divided in the following groups: control, diabetes (DM), sepsis (CLP) and sepsis/diabetes (DM+CLP). Plasma was collected for the Enzyme Linked Immuno Sorbent Assay test (ELISA) and the cells were used for Ribonucleic Acid extraction (RNA) that was then analyzed through the Polymerase Chain Reaction (PCR-array). RESULTS: Our mortality curve showed significant differences among the study groups (p=0.04). We found that the CLP vs DM+CLP groups showed significant differences in the mRNA expression of the lipoproteins Fabp4 in adipocytes (p=0.0095) and myocytes (p=0.0152), Acat2 in myocytes (p=0.0303) and Gk in leukocytes (p=0.0260). The protein values of Fabp7 (p=0.0152) exhibited increased plasma concentrations in diabetic animals submitted to sepsis (DM+CLP). CONCLUSION: We conclude that our results of the lipoprotein expression values of several molecules reveal key findings in the pathogenesis of sepsis in diabetic animals and can be used as biomarkers of sepsis in this specific population
Ricci, Pierbruno. "The Renal Cysts and Diabetes syndrome : from transcriptional profiling and functional analysis of a novel mouse model to biomarkers evaluation in human patients." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS111/document.
Повний текст джерелаHeterozygous mutations in the gene encoding the transcription factor HNF1B are the cause of a complex multisystem syndrome known as Renal Cysts And Diabetes (RCAD). A mouse model generated in our laboratory was shown to reproduce several features of the human disease. We performed high-throughput mRNA-microRNA sequencing at different developmental stages (E14.5, E15.5, E17.5). We showed that the most down-regulated genes were involved in transport, lipid and organic acid metabolic processes and expressed in proximal tubules and to a lesser extent in the loop of Henle and collecting ducts. We then selected four microRNAs (mir-802, 194-2, 192 and -30a), which were down-regulated and potentially controlled by HNF1B. Luciferase assays in HEK-293 cells showed that HNF1B was able to specifically transactivate in a dose response mode these microRNAs through binding HNF1B-binding sites in their regulatory promoter/enhancer upstream sequences. We subsequently showed by luciferase assays using miRNA MIMICS that mir-802, mir-194-2 and mir-192 were able to inhibit luciferase vectors containing the 3’UTR of Hnf1b. Analysis of urine samples from 22 RCAD patients and 22 healthy controls led to the identification of 146 peptides differentially excreted and associated with RCAD including a similarity regarding collagen and uromodulin fragments with the RCAD mouse model. Combining the peptides into a mathematical model we used independent cohorts of patients to validate the prediction of the RCAD syndrome. Our classifier efficiently predicted RCAD syndrome with 91.7% sensitivity and 91.1% specificity on a wide population
Köhler, Meike [Verfasser], and Sonja [Akademischer Betreuer] Greven. "Flexible Bayesian joint models for longitudinal biomarkers and time-to-event outcomes with applications to type 1 diabetes research / Meike Köhler ; Betreuer: Sonja Greven." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1142113728/34.
Повний текст джерелаDib, João Mariana Ferreira 1987. "Relationship between the presence of periodontal pathogens and plasma biomarkers in development of periodontal disease in children with type I diabetes mellitus = Relação entre a presença de periodontopatógenos e os níveis de biomarcadores plasmáticos no desenvolvimento da doença periodontal em crianças portadoras de diabetes melito tipo I." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290597.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-23T19:41:09Z (GMT). No. of bitstreams: 1 DibJoao_MarianaFerreira_M.pdf: 1014789 bytes, checksum: 02391fe3292ee31afa12160829713a12 (MD5) Previous issue date: 2013
Resumo: A doença periodontal compreende um grupo de infecções inflamatórias que afeta os tecidos periodontais, podendo ser desencadeada por múltiplos fatores locais e sistêmicos. Indivíduos de todas as faixas etárias são susceptíveis ao desenvolvimento dessa doença e a gengivite, condição reversível limitada aos tecidos gengivais, é comumente vista em crianças e no início do período da adolescência. A presença de uma microbiota patogênica específica é um fator essencial para o desenvolvimento da doença periodontal (DP). Doenças sistêmicas tais como diabetes melito, podem contribuir para o desenvolvimento da doença, principalmente devido à resposta exacerbada do sistema imunológico e alterações em parâmetros fisiológicos que contribuem para a agressão tecidual. O objetivo deste estudo foi comparar os aspectos clínico, microbiológico e imunológico de crianças portadoras de diabetes melito tipo I (DM) com crianças não diabéticas (NDM). Vinte e quatro pacientes diabéticos e vinte e sete normoglicêmicos foram avaliados. As condições bucais foram avaliadas através dos índices de placa, gengival e profundidade de sondagem e o perfil de saúde geral dos pacientes foi avaliado através dos níveis de glicemia, HbA1c (hemoglobina glicosilada), triglicerídeos (TRG), colesterol total (CT), HDL, LDL, VLDL e lipídeos totais (LT), a partir de amostras sanguíneas. O método do PCR foi utilizado para identificação das seguintes bactérias: Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Treponema denticola (Td) (complexo vermelho), Prevotella intermedia (Pi) e Prevotella nigrescens (Pn), .Fusobacterium nucleatum (Fn), Campylobacter rectus (Cr) (complexo laranja), Capnocytophaga sputigena (Cs), Capnocytophaga ochracea (Co), Eikenella corrodens (Ec) (complexo verde) em amostras do sulco gengival de dentes decíduos e permanentes. A avaliação imunológica incluiu a detecção dos níveis dos biomarcadores inflamatórios IL- 1?, TNF-? e IL-6 através de ensaios de ELISA. Os dados foram submetidos à análise estatística considerando p? 0,05. Os resultados mostraram que a condição periodontal de pacientes diabéticos e não diabéticos foi similar. Quando considerados pacientes com gengivite (IG ? 2), todos os índices lipídicos avaliados foram maiores no grupo dos diabéticos, com diferença estatística para HDL, TRG e LT. A prevalência do "complexo verde", principalmente Cs e Co foi maior nos sítios periodontais de crianças diabéticas. Bactérias do "complexo vermelho" foram detectadas em poucos sítios dos grupos DM e NDM. Fn e Cr, do "complexo laranja", foram frequentemente encontrados em ambos os grupos. Níveis dos biomarcadores IL-1-?, TNF-? e IL-6 foram similares no soro de DM e NDM. Houve correlação positiva entre as variáveis lipídicas e imunológicas avaliadas somente para o grupo diabético. Conclui-se que os perfis periodontal, microbiológico e imunológico avaliados neste estudo foram similares entre as crianças diabéticas e não diabéticas. Os parâmetros glicêmicos e lipídicos foram maiores nos pacientes diabéticos, mas mantiveram-se dentro da normalidade, demonstrando que controle metabólico é essencial para a manutenção da saúde periodontal
Abstract: Periodontal disease (PD) comprises a group of inflammatory infections that affects the periodontal tissues and may be triggered by multiple local and systemic factors. Individuals of all age groups are susceptible to develop this disease and gingivitis, reversible condition limited to the gingival tissues, is commonly seen in children and in the early adolescence period. The presence of a specific pathogenic microbiota is an essential factor to PD development. Systemic diseases, such as diabetes mellitus, can increase the development and progression of the disease, mainly due to exacerbated immunological response and changes in physiological parameters that contribute to tissue injury. The aim of this study was to compare clinical, microbiological and immunological profiles of type 1 diabetes mellitus children (DM) to non-diabetic control group (NDM). A total of twenty four DM children and twenty seven NDM controls were evaluated. Periodontal status was assessed using plaque index, gingival index and probing depth and general health status were determined using glycemic levels, HBA1c (glycosylated haemoglobin), HDL, LDL, triglycerides (TRG), total cholesterol (TC), VLDL and total lipids (TL), from blood samples. Polymerase chain reaction (PCR) was used for identification of the following bacteria: Aggregatibacter actinomycetemcomitans (Aa), Campylobacter rectus (Cr), Capnocytophaga sputigena (Cs), Capnocytophaga ochracea (Co), Eikenella corrodens (Ec), Fusobacterium nucleatum (Fn), Tannerella forsythia (Tf), Treponema denticola (Td), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) e Prevotella nigrescens (Pn) from gingival crevicular fluid of deciduous and permanent teeth. Immunological evaluation was determined by means of detection of inflammatory biomarkers -1?, TNF-? and IL-6 using ELISA assays. Data were submitted to statistical analysis, considering p? 0.05. The results showed that periodontal status of diabetic and non-diabetic patients was similar. Considering patients with gingivitis (GI ? 2), all lipid parameters evaluated were highest in DM group, however, statistical difference was observed only for HDL, TRG and TL. The prevalence of "green complex", mainly Cs and Co, was definitely more prevalent in periodontal sites of DM children. Bacteria from "red complex" were detected in few sites of DM and NDM groups. From the "orange complex", Fn and Cr were frequently found in both groups. Similar levels of the serum biomarkers, IL-1-?, TNF-? and IL-6, were detected in the serum of DM and NDM children. In conclusion, clinical, microbiological and immunological profiles evaluated in this study were similar between diabetic and nondiabetic children. Glycemic and lipid parameters were higher in diabetic patients, but remained within normal values, demonstrating that metabolic control is essential for maintaining periodontal health
Mestrado
Microbiologia e Imunologia
Mestra em Biologia Buco-Dental
Ayepola, Omolola Rebecca. "Effects of kolaviron–a Garcinia kola biflavonoid on biochemical and histological parameters in streptozotocin - induced diabetes and diabetic complications (nephrotoxicity and hepatotoxicity) in male Wistar rats." Thesis, Cape Peninsula University of Technology, 2014. http://hdl.handle.net/20.500.11838/1512.
Повний текст джерелаDiabetes mellitus (DM) results in severe metabolic imbalances and pathological changes in many tissues. Chronic inflammation and oxidative stress have been implicated in the pathophysiology of diabetes mellitus. Garcinia kola (Family: Guttiferae) is a plant well known for its ample medicinal values. The seed of the plant also known as ‘bitter kola’ due to its bitter taste is used as a masticatory agent in traditional hospitality, cultural and social ceremonies in Africa. Kolaviron (KV) is a defatted ethanol extract from the seeds of Garcinia kola (GK). Kolaviron has been shown in experimental models of diseases to have numerous beneficial effects due to the presence of flavonoids (mainly Garcinia biflavonoid (GB)-1, GB-2 and kolaflavanone). However, there is paucity of information regarding the possible effect of kolaviron on inflammatory mediators and oxidative stress in diabetes mellitus. Therefore, this study was carried out to investigate the potential beneficial effects of kolaviron on antioxidant status, inflammatory mediators and apoptosis. Other biochemical and histological alterations in the blood, liver and kidney of streptozotocin-induced diabetic rats were also evaluated. A single intraperitoneal injection of freshly prepared solution of streptozotocin (50 mg/kg.b.wt.) in citrate buffer (0.1M, pH 4.5) was administered to overnight fasted rats for diabetes induction. Diabetes was confirmed by stable hyperglycemia (>18 mmol/l) in the tail blood glucose after 5 days of streptozotocin injection. Kolaviron (100 mg/kg b.wt.) was administered to diabetic rats (by gastric gavage) on the 6th day after the induction of diabetes and treatment continued for 6 weeks (5 times weekly). The effects on blood glucose, body weight, organ (liver and kidney) weight, serum biochemical parameters, oxidative status, inflammatory mediators and histology of the liver, kidney and pancreas were assessed. Kolaviron (KV) treatment lowered blood glucose in diabetic and normoglycemic rats and reduced glycated haemoglobin [HbA1C (%)]. Plasma insulin level was raised in diabetic rats treated with KV. Histomorphometric analysis of the pancreas revealed increased β-cell area of pancreatic islets of kolaviron-treated diabetic group. The indices of organ (liver and kidney) damage were increased in diabetic rats. However, KV treatment protected against liver and kidney damage. The characteristic features of diabetic dyslipidemia such as elevated serum triglyceride and cholesterol concentration which are major risk factors for cardiovascular disease were also significantly reduced in KV-treated diabetic rats. Alteration in antioxidant enzymes status was observed in the liver, kidney and blood (erythrocyte, plasma and serum) of diabetic rats. Lowered catalase (CAT) activity was observed in the liver and kidney of diabetic rats while KV treatment significantly (p < 0.05) elevated catalase activity in the liver and kidney. There was no significant change (p > 0.05) in erythrocyte catalase activity among all treatment groups. Erythrocyte of diabetic rats showed a marked reduction in the activity of superoxide dismutase (SOD) with no significant changes in liver and kidney SOD activity of diabetic rats compared to control whereas KV administration to rats markedly increased SOD activity. Glutathione peroxidase (GPX) activity was elevated in the erythrocyte and kidney of STZ-induced diabetic rats with no significant effect on liver GPX activity. KV treatment reversed the alteration in GPX activity in the kidney and erythrocyte. Level of reduced glutathione (GSH), a non-enzymatic antioxidant was decreased in the both liver and kidney of diabetic rats and treatment of diabetic rats with KV elevated GSH concentration in both tissues. Also, malondialdehyde (MDA), a marker of lipid peroxidation was elevated in the liver, kidney and plasma of diabetic rats and significantly (p < 0.05) lowered following KV treatment. Diabetes induction reduced the capacity of liver and kidney to absorb oxygen radicals as demonstrated by lowered oxygen radical absorbance capacity (ORAC) values. KV administration to normal and diabetic rats significantly increased ORAC values. Increased rate of apoptosis, a major cellular response to high glucose induced stress was observed in the renal and hepatic tissues of diabetic control rats. Kolaviron treatment of diabetic rats protected the liver and kidney against hyperglycemia-induced apoptosis and decreased the number of TUNEL positive cells A significant (p < 0.05) elevation of pro-inflammatory cytokines; monocyte chemoattractant protein (MCP-1), Interleukin-1β (IL-1β), IL-6 and tumor necrosis factor (TNF)-𝛂 was observed in the liver of diabetes rats. KV treatment lowered these inflammatory biomarkers. On the other hand, the kidney of diabetic rats showed elevated concentration of pro-inflammatory IL-1β with no significant effect on kidney TNF-𝛂. An increase in the serum concentration of MCP-1 and IL-1β was observed in the untreated diabetic rats while kolaviron treatment normalized the alteration in serum concentration of MCP-1, IL-1β and vascular endothelial growth factor (VEGF). In conclusion, persistent and chronic hyperglycemia promotes the generation of free radicals and inflammatory molecules which contributes to progressive development of micro- and macro vascular complications and multi-organ damage. Kolaviron demonstrated beneficial effects on markers of oxidative stress and inflammation in the diabetic rats and also promoted the survival and functional integrity of the liver and kidney.
Chiyoda, Alberto. "Programa de exercícios físicos : análise dos fatores de risco cardiovaculares e biomarcadores de inflamação e estresse oxidativo em diabéticas exercitadas e não exercitadas /." Rio Claro : [s.n.], 2011. http://hdl.handle.net/11449/87367.
Повний текст джерелаBanca: Eduardo Kokubun
Banca: Fúlvia de Barros Manchado Gobatto
Resumo: As doenças cardiovasculares (DC) são a principal causa de morte e inabilidade em diabéticos, o que gera enormes custos em saúde pública. Os problemas cardiovasculares afetam mulheres diabéticas de forma ainda mais pronunciada que em homens. Por outro lado, crescentes evidências reafirmam a importância da atividade física (AF) no tratamento do DM e para a saúde cardiovascular. Assim, objetivo do trabalho foi comparar diabéticas exercitadas e não exercitadas em relação aos fatores de risco cardiovasculares e biomarcadores de inflamação e estresse oxidativo. A amostra foi composta por mulheres diabéticas do tipo 2 com idade acima de 40 anos divididas em dois grupos: exercitadas (E) (n=21) e nãoexercitadas (N) (n= 16). O grupo E participou do programa de exercícios físicos de intensidade moderada por mais de seis meses, duas vezes por semana com duração de uma hora por sessão. Para o tratamento estatístico foi aplicado o teste de Shapiro-Wilk e Kolmogorov Smirnov para verificar a normalidade dos dados. As variáveis que não apresentaram dados normais foi utilizado o teste de Mann Whitney e para as variáveis que apresentaram dados normais foi utilizado o Teste t para amostras independentes. Em relação às variáveis, estatura (E: 1,53 ± 0,06 m; N: 1,58 ± 0,07 m), peso (E: 74,6 ± 14,4 kg; N: 71,4 ± 14,6 kg), IMC (E: 30,8 ± 5,8 kg/m²; N: 28,6 ±4 ,8 kg/m²), circunferência de cintura (E: 97,5 ± 12,4 cm ; N: 97,2 ± 10,9), relação cintura/quadril (E: 0,9 ± 0,1; N: 0,9 ± 0,08), pressão arterial sistólica (E: 131,9 ± 19,4 mmHg; N: 126,9 ± 15,8 mmHg) e pressão arterial diastólica (E: 79,7 ± 11,9 ; N: 79,4 ± 12,4) os grupos estudados foram semelhantes estatisticamente. Para as variáveis metabólicas, glicemia (E: 157,4 ± 75,6 mg/dl; N: 139,1 ± 69,5 mg/dl ), HDL (E: 54,4 ± 19,4 mg/dl ; N: 56,3 ± 25,7 mg/dl ), TG ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Cardiovascular diseases are the main cause of death and inability in diabetics, and it generates high costs in public health. The cardiovascular problems affect mostly women. Besides that, lately evidences confirms the importance of the physical activity in the treatment of diabetes and for the cardiovascular health in general. Thus, the purpose of the study was to compare exercised and not exercised diabetic women regarding cardiovascular risks factors and biomarkers of inflammation and oxidative stress. The sample was composed of type 2 diabetics women aging over 40 years old, separated by two groups: exercise (E) (n=21, age= 62,5 ± 11,9 years) and not exercised (N) (n= 16, age: 57,2 ± 7,2 years). Group E has participated of a moderated physical activities program over six months, on a one hour session, twice a week. For the statistics was applied the test of Shapiro-Wilk and Kolmogorov Smirnov in order to verify the accuracy of the data. For the ones that had not presented normal data was used the test of Mann Whitney and for ones that had presented normal data test t was applied for independent samples. In relation to the variables, stature (E: 1,53 ± 0,06 m; N: 1,58 ± 0,07 m), weight (E: 74,6 ± 14,4 kg; N: 71,4 ± 14,6 kg), BMI (E: 30,8 ± 5,8 kg/m²; N: 28,6 ±4 ,8 kg/m²), waist cincumference (E: 97,5 ± 12,4 cm ; N: 97,2 ± 10,9), waist/rip ratio (E: 0,9 ± 0,1; N: 0,9 ± 0,08), systolic blood pressure (E: 131,9 ± 19,4 mmHg; N: 126,9 ± 15,8 mmHg) and diastolic blood pressure (E: 79,7 ± 11,9 ; N: 79,4 ± 12,4) the studied groups had similar statistics.For the metabolics variables, glucose (E: 157,4 ± 75,6 mg/dl; N: 139,1 ± 69,5 mg/dl), HDL (E: 54,4 ± 19,4 mg/dl ; N: 56,3 ± 25,7 mg/dl ), TG (E: 137,0 ± 85,0 mg/dl ; N: 136,6 ± 65,3 mg/dl), TBARS (E: 24,3 ± 2,3 μmol/L; N: 22,7 ± 2,4 μmol/L) and C-reactive protein (E: 3,6 ± 0,6 log; N: 3,7 ± 0,6 log) no differences ... (Complete abstract click electronic access below)
Mestre
Saldarriaga, Magda Elizabeth Graciano. "Pesquisa de miRNAs circulantes, potenciais biomarcadores de aterosclerose subclínica em indivíduos euglicêmicos e pré-diabéticos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-24052017-161009/.
Повний текст джерелаCardiovascular Disease and Diabetes Mellitus are relevant NCDs for the WHO. It´s estimated that, worldwide, there are 387 millions of diabetics and 316 millions of people with risk characteristics like prediabetes. About 60% of patients with T2DM develops cardiovascular disease, which starts at the same time as disorders of glucose metabolism, there may be common pathophysiological mechanisms among diseases. Half of coronary events, including sudden death, occurs in asymptomatic individuals. This fact, highlights the need for new early markers of the disease, especially in asymptomatic patients, since in many of them, death is the first manifestation. It has been recently suggested that miRNAs involved in pos-transcriptional regulation of gene expression, could be characterized as biomarkers of diseases. Our goal is to identify changes in the profile of circulating microRNAs in euglycemic and prediabetic patients with or without subclinical atherosclerosis, by quantitative Polymerase Chain Reaction array (qPCR Array) in order to find molecular biomarkers of this condition. We found that subclinical atherosclerosis was associated with aging, menopause, white ethnicity, dyslipidemia, insulin resistance, increased adiposity, leptin and TNF-α. The up-regulation of miR98-5p and the down-regulation miR-212-3p, miR-145-5p, miR-93-5p, miR15a-5p, miR-19a-3p, miR32-5p led to activation of the signaling pathway of atherosclerosis. The results suggest that inflammation was the main mechanism associated with the development of subclinical atherosclerosis in this study.
Chiyoda, Alberto [UNESP]. "Programa de exercícios físicos: análise dos fatores de risco cardiovaculares e biomarcadores de inflamação e estresse oxidativo em diabéticas exercitadas e não exercitadas." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/87367.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
As doenças cardiovasculares (DC) são a principal causa de morte e inabilidade em diabéticos, o que gera enormes custos em saúde pública. Os problemas cardiovasculares afetam mulheres diabéticas de forma ainda mais pronunciada que em homens. Por outro lado, crescentes evidências reafirmam a importância da atividade física (AF) no tratamento do DM e para a saúde cardiovascular. Assim, objetivo do trabalho foi comparar diabéticas exercitadas e não exercitadas em relação aos fatores de risco cardiovasculares e biomarcadores de inflamação e estresse oxidativo. A amostra foi composta por mulheres diabéticas do tipo 2 com idade acima de 40 anos divididas em dois grupos: exercitadas (E) (n=21) e nãoexercitadas (N) (n= 16). O grupo E participou do programa de exercícios físicos de intensidade moderada por mais de seis meses, duas vezes por semana com duração de uma hora por sessão. Para o tratamento estatístico foi aplicado o teste de Shapiro-Wilk e Kolmogorov Smirnov para verificar a normalidade dos dados. As variáveis que não apresentaram dados normais foi utilizado o teste de Mann Whitney e para as variáveis que apresentaram dados normais foi utilizado o Teste t para amostras independentes. Em relação às variáveis, estatura (E: 1,53 ± 0,06 m; N: 1,58 ± 0,07 m), peso (E: 74,6 ± 14,4 kg; N: 71,4 ± 14,6 kg), IMC (E: 30,8 ± 5,8 kg/m²; N: 28,6 ±4 ,8 kg/m²), circunferência de cintura (E: 97,5 ± 12,4 cm ; N: 97,2 ± 10,9), relação cintura/quadril (E: 0,9 ± 0,1; N: 0,9 ± 0,08), pressão arterial sistólica (E: 131,9 ± 19,4 mmHg; N: 126,9 ± 15,8 mmHg) e pressão arterial diastólica (E: 79,7 ± 11,9 ; N: 79,4 ± 12,4) os grupos estudados foram semelhantes estatisticamente. Para as variáveis metabólicas, glicemia (E: 157,4 ± 75,6 mg/dl; N: 139,1 ± 69,5 mg/dl ), HDL (E: 54,4 ± 19,4 mg/dl ; N: 56,3 ± 25,7 mg/dl ), TG...
Cardiovascular diseases are the main cause of death and inability in diabetics, and it generates high costs in public health. The cardiovascular problems affect mostly women. Besides that, lately evidences confirms the importance of the physical activity in the treatment of diabetes and for the cardiovascular health in general. Thus, the purpose of the study was to compare exercised and not exercised diabetic women regarding cardiovascular risks factors and biomarkers of inflammation and oxidative stress. The sample was composed of type 2 diabetics women aging over 40 years old, separated by two groups: exercise (E) (n=21, age= 62,5 ± 11,9 years) and not exercised (N) (n= 16, age: 57,2 ± 7,2 years). Group E has participated of a moderated physical activities program over six months, on a one hour session, twice a week. For the statistics was applied the test of Shapiro-Wilk and Kolmogorov Smirnov in order to verify the accuracy of the data. For the ones that had not presented normal data was used the test of Mann Whitney and for ones that had presented normal data test t was applied for independent samples. In relation to the variables, stature (E: 1,53 ± 0,06 m; N: 1,58 ± 0,07 m), weight (E: 74,6 ± 14,4 kg; N: 71,4 ± 14,6 kg), BMI (E: 30,8 ± 5,8 kg/m²; N: 28,6 ±4 ,8 kg/m²), waist cincumference (E: 97,5 ± 12,4 cm ; N: 97,2 ± 10,9), waist/rip ratio (E: 0,9 ± 0,1; N: 0,9 ± 0,08), systolic blood pressure (E: 131,9 ± 19,4 mmHg; N: 126,9 ± 15,8 mmHg) and diastolic blood pressure (E: 79,7 ± 11,9 ; N: 79,4 ± 12,4) the studied groups had similar statistics.For the metabolics variables, glucose (E: 157,4 ± 75,6 mg/dl; N: 139,1 ± 69,5 mg/dl), HDL (E: 54,4 ± 19,4 mg/dl ; N: 56,3 ± 25,7 mg/dl ), TG (E: 137,0 ± 85,0 mg/dl ; N: 136,6 ± 65,3 mg/dl), TBARS (E: 24,3 ± 2,3 μmol/L; N: 22,7 ± 2,4 μmol/L) and C-reactive protein (E: 3,6 ± 0,6 log; N: 3,7 ± 0,6 log) no differences ... (Complete abstract click electronic access below)
Eendebak, Robert. "The potential relationships between hormone biomarkers and functional and health outcomes of ageing." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/the-potential-relationships-between-hormone-biomarkers-and-functional-and-health-outcomes-of-ageing(e28321cc-703c-44df-99b4-fb0d76f7f429).html.
Повний текст джерелаSomineni, Hari Krishna. "Physical exercise training but not metformin attenuates albuminuria and shedding of ACE2 in type 2 diabetic db/db mice." Wright State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=wright1369704228.
Повний текст джерелаVinagre, Torres Irene. "Biomarcadores de la inflamación en la diabetes mellitus tipo 2: Efecto del control glucémico y del fenotipo de las LDL." Doctoral thesis, Universitat Autònoma de Barcelona, 2014. http://hdl.handle.net/10803/285417.
Повний текст джерелаCardiovascular diseases are the most prevalent complications in patients with type 2 diabetes mellitus (T2DM) and are associated with increased morbidity and mortality. The mechanisms responsible for the accelerated development of atherosclerosis in patients with T2DM are not known with precision, but both atherogenic dyslipidemia and low-grade inflammation characteristics of this population, are involved. There is limited information on the relationship between the degree of glycemic control and quality characteristics of low-density lipoproteins (LDL) and high-density lipoproteins (HDL) and also the effect that the optimization of glycemic control could have on the quality characteristics of LDL and HDL particles and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) is unknown. Similarly, studies that attempt to determine the correlation between inflammatory parameters and glycemic control are not entirely conclusive, and information on the effect of improved glycemic control on inflammatory parameters is also scarce. Thus, our hypothesis is that the intensification of glycemic control provides benefits due to inflammatory parameters modification and qualitative changes in lipoproteins and distribution of Lp-PLA2, which could help to reduce cardiovascular risk in patients with T2DM. We have developed a cross-sectional study of 122 patients with T2DM and poor glycemic control (glycosylated hemoglobin (HbA1c) > 8,5%) and 54 control subjects, and a longitudinal study with a subgroup of 42 patients, which were optimized with different therapeutic strategies and followed for 3 months. There has been a clinical characterization of the subjects (age, sex, weight, body mass index, duration of T2DM, previous medication, chronic complications), determination of the lipid profile (total cholesterol, lipoprotein cholesterol fractions, LDL size, qualitative characteristics of LDL and HDL, triglycerides, apolipoprotein AI, apolipoprotein B, free fatty acids) and inflammatory biomarkers (C-reactive protein, interleukin-6, interleukin-8, monocyte chemotactic protein 1, transforming growth factor β1 (TGF -β1), adiponectin, leptin). We compared these aspects between the different groups studied and we assessed the improvement of glycemic control (HbA1c) on the lipid profile, inflammatory parameters and qualitative characteristics of lipoproteins. The main findings of this thesis are: 1- Switching to basal-bolus insulin therapy is feasible, effective and safe in patients with long-standing T2DM and poor glycemic control, and does not impair their quality of life; 2- Patients with T2DM have alterations in the qualitative and quantitative characteristics of LDL and HDL and many of them are related to phenotype B of LDL particles; 3- Improvement of glycemic control promotes less atherogenic changes in LDL and HDL particles; 4- Inflammatory biomarkers are elevated in T2DM subjects, especially in those with phenotype B; 5- Optimization of glycemic control may reduce levels of TGF-β1 in part involved in the pathogenesis of diabetic nephropathy. Thus, subjects with T2DM, especially those with LDL phenotype B, have elevated levels of inflammatory biomarkers and qualitative changes in LDL and HDL particles associated with increased atherogenicity. The intensification of glycemic control brings benefits that could help to reduce cardiovascular risk in patients with T2DM.
Tegg, Michelle. "Plasma insulin-degrading enzyme: Characterisation and evaluation as a potential biomarker for Alzheimer's disease." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2014. https://ro.ecu.edu.au/theses/1198.
Повний текст джерелаBanerjee, Srikanta. "Inflammatory Markers Associated With Disease Progression of Cardiorenal Syndrome." ScholarWorks, 2015. https://scholarworks.waldenu.edu/dissertations/1433.
Повний текст джерелаLundin, Ulrika. "Biomarker Discovery in Diabetic Nephropathy by Targeted Metabolomics." Thesis, Linköping University, The Department of Physics, Chemistry and Biology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15640.
Повний текст джерелаDiabetic nephropathy is a chronic kidney disease and one of the more severe complications from diabetes mellitus type 2. The glomerular and tubular dysfunctions usually lead to end stage renal disease and the treatments of these patients (dialysis, kidney transplants) are a huge economic burden for the society. Due to an epidemiologic increase of type 2 diabetes, conventional diagnostic markers like creatinine and albumin are not sufficient, since they are only able to identify already existing kidney damage. With targeted metabolomics, the analysis of small molecules produced from metabolism, this project aimed at finding novel and more sensitive metabolic biomarkers from several different classes of metabolites. The different assays were performed with flow injection analysis, high performance liquid chromatography, gas chromatography and mass spectrometry, and with principal component analysis and discriminant analysis, up-and down-regulated metabolites could be identified and their respective biochemical pathways, if possible, explained. In diabetics significantly elevated concentrations of very long chain fatty acids (impaired peroxisomal β-oxidation), urinary sugars and acylcarnitines in plasma could be recognized. Markers indicating kidney damage included significantly increased plasma concentrations of asymmetric dimethylarginine (inhibition of nitric oxide synthase resulting in decreased endothelial functionality) and histamine (indication of uremic pruritus). Oxidative stress was also found to be a potential prognostic marker as indicated by the raised methionine-sulfoxide to methionine ratio in nephrotic patients. To summarize, this project succeeded in identifying metabolic biomarkers both for diabetes type 2 and nephropathy, which in the future might become important tools in slowing down progression or diagnosing these diseases.
Шандиба, Ірина Олександрівна, Ирина Александровна Шандыба, Iryna Olexandrivna Shandyba, Андрій Миколайович Лобода, Андрей Николаевич Лобода, and Andrii Mykolaiovych Loboda. "Early diagnosis of diabetic nephropathy in children with type 1 diabetes mellitus using the VCAM-1 biomarker." Thesis, Lithuanian University of Health Sciences, 2020. https://essuir.sumdu.edu.ua/handle/123456789/78327.
Повний текст джерелаВаскулярная молекула клеточной адгезии-1 (VCAM-1) - это 90 кДа гликопротеин, который экспрессируется в эндотелиальных клетках и участвует в миграции и рекрутировании воспалительных клеток. Недавние исследования показали, что уровни VCAM-1 в моче были значительно повышены у пациентов с заболеванием почек. Целью настоящего исследования было изучение особенностей уровней VCAM-1 в моче детей в зависимости от продолжительности диабета. В исследование были включены 47 детей с диабетом 1-го типа и 8 детей без диабета. VCAM-1 в моче увеличился на 24 процента у детей с продолжительностью диабета менее одного года по сравнению с контрольной группой. Уровни VCAM-1 были повышены на 33 процента у детей с продолжительностью диабета от одного до пяти лет. Этот показатель увеличился на 54 процента у детей, которые жили с диабетом более пяти лет. Выводы. Увеличение уровня VCAM-1 в моче наблюдалось уже в первый год манифестации диабета у детей. Измерение уровня VCAM-1 в моче может быть полезно для ранней диагностики диабетической нефропатии.
Vascular cell adhesion molecule-1 (VCAM-1) – is a 90-kDa glycoprotein that is expressed in endothelial cells and is involved in the migration and recruitment of inflammatory cells. Recent studies have shown that urinary VCAM-1 levels were significantly increased in patients with kidney disease. The aim of the current study was to investigate the features VCAM-1 levels in urine of children depending on the diabetes duration. Study included 47 children with 1 type diabetes mellitus and 8 children without diabetes. VCAM-1 in urine increased by 24 percent in children with the duration of diabetes below one year compared to the control group. VCAM-1 levels were elevated by 33 percent in children with the duration of diabetes from one to five years. The marker increased by 54 percent in children who lived with diabetes for more than five years. Conclusions. Increase in urinary VCAM-1 was observed in the first year of diabetes in children. Measuring the level of VCAM-1 in urine may be useful for the early diagnosis of diabetic nephropathy.
Thanks for the research group of Thomas Boren (Department of Medical Biochemistry and Biophysics/MIMS, Umea University) for the opportunity to conduct research in framework of collaboration in Erasmus+ (KA1) programme, 2018/2019. The authors declare absence of potential conflicts of interest.
Crawford, Michelle Anne. "Assessment of glycated insulin as a novel clinical biomarker for pre-diabetes and diabetes." Thesis, University of Ulster, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588748.
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