Статті в журналах з теми "Denise Barbou"

We give a minimum degree condition sufficient to ensure the existence of a fractionalKr-decomposition in a balancedr-partite graph (subject to some further simple necessary conditions). This generalizes the non-partite problem studied recently by Barber, Lo, Kühn, Osthus and the author, and the 3-partite fractionalK3-decomposition problem studied recently by Bowditch and Dukes. Combining our result with recent work by Barber, Kühn, Lo, Osthus and Taylor, this gives a minimum degree condition sufficient to ensure the existence of a (non-fractional)Kr-decomposition in a balancedr-partite graph (subject to the same simple necessary conditions).

This is a review of the book „Elemente de drept internațional public” [Elements of public international law], author – Denisa Barbu, Published by Lumen Publishing, from Iași, Romania, in 2022. The book is a university course, both theoretical and practical, written by an experienced researcher and law scholar, which can be a valuable tool not only for students, even if it is particularly addressed to them, but also to law practitioners.

The topic of this book review is the University Course entitled "Criminal Procedure Law. General Part", authored by Lecturer PhD Denisa Barbu. The reviewed university course represents a real successful didactic work of the author and was published by Lumen Publishing House from Iași, in 2016. From the beginning of the review, it is worth noting that the analysis of the course shows an essential conclusion, namely that the paper is an indispensable tool for students / masters / doctoral students, but also for all categories of legal practitioners, offering the advantage of an absolutely necessary update of criminal law, by reference to the most recent legislation into force.

Ethnic mongol herders in north china have traditionally enjoyed a distinctive cultural landscape. In contrast to the way dense population and intensive agriculture strictly regimented land-use practices among Han Chinese, dispersed settlement and mobile stock-herding permitted Mongol society to operate and evolve under much different spatial and ecological horizons. Such deep-rooted orientations still resist facile alignment with alien standards now imposed by Beijing, disguised as they are in the language of economic development.

Real-world relations are often represented as bipartite networks, such as paper-author networks and event-attendee networks. Extracting dense subnetworks (communities) from bipartite networks and evaluating their qualities are practically important research topics. As the attempts for evaluating divisions of bipartite networks, Guimera and Barber propose bipartite modularities. This paper discusses the properties of these bipartite modularities and proposes another bipartite modularity that allows one-to-many correspondence of communities of different vertex types.

The cirri of the digital tentacles ofNautilus pompiliusare covered by annular ridges, more pronounced on the oral (adhesive) than the aboral side. On the oral side the epithelium is thicker on the proximal and outer surfaces of the ridges than on their distal surfaces. Prominent electron-dense granules occur in the cells of the thick epithelium, but are absent from the thin epithelium and the epithelium of the aboral surface. These granules contain mucopolysaccharide and may be responsible for adhesion.The digital tentacles ofNautilusare used for attachment to, for example, prey, the substratum, or the partner's shell during mating. Their general structure and histology have been described by Owen (1832), Willey (1898), Barber & Wright (1969), Fukuda (1987) and Kier (1987). The mechanism of adhesion is still uncertain. Barber & Wright (1969) report epithelial cells of two types: pigmented cells containing pigment granules 0–5–1 µm in diameter, interspersed with a small number of mucus-producing cells which may help with the adhesive process.

RÉSUMÉCe livre explore la façon dont les changements du fonctionnement cognitif et communicatif reliés à l'âge influent sur le fondement des déclarations d'un sujet. Il comble done l'écart entre deux domaines de recherche non encore relies. Le premier domaine constitue le territoire des psychologues de la cognition et du vieillissement et concerne la façon dont le fonctionnement cognitif et communicatifse modifie auec les étapes de la vie. Le deuxième résulte des travaux des spécialistes de la méthodologie de recherche et des psychologues socio-cognitifs et concerne le fonctionnement cognitif et communicatif sur lequel repose les déclarations des sujets. Ces travaux présentent un intérêt indéniable pour les gérontologues qui se fient sur les déclarations des sujets de recherche dans l'établissement de leurs conclusions. En sachant de quelle façon les déclarations sont influencées par les changements cognitifs reliés à l'âge, il devient possible de modifier les modalités de collecte de données pour répondre aux besoins des répondants plus âgés. Il pourrait également devenir possible d'interpréter les données avec plus de précision.Ce livre est excellent. Il est écrit dans un langage accessible au profane, va droit au but et la grande majorité du contenu présente une qualité indéniable. Les chercheurs le trouveront précieux étant donné qu'il contient une grande quantité de suggestions de sondages et de conseils sur la conception et l'administration de questionnaires. De plus, plusieurs chapitres contiennent des idées de recherches intéressantes.

Background: Due to the anatomical complexity of the root canal system, irrigation plays an essential role in endodontics. This in vitro study was sought to compare the removal of the smear layer (RSL) promoted by conventional irrigation (CI), passive ultrasonic irrigation (PUI) and continuous ultrasonic irrigation (CUI) with 17% EDTA, by using scanning electron microscopy (SEM). Material and Methods: Forty single-rooted human mandibular canines were instrumented and randomly assigned to four groups (n=10), according to the irrigation protocol aiming to the RSL: CG (control group) —conventional irrigation with distilled water; CI— conventional irrigation with 17% EDTA; PUI —passive ultrasonic irrigation with 17% EDTA; CUI— continuous ultrasonic irrigation with 17% EDTA. Hemisections from each sample were obtained, and images of each root canal third (cervical, middle and apical) were captured at 1000 X magnification by SEM. Three previously calibrated and blinded evaluators classified the RSL, according to the criteria proposed by Torabinejad et al.: small or no smear layer (all dentinal tubules were clean and open); 2 = moderate smear layer (no smear layer on the surface of root canal, but dentinal tubules contained debris); 3 = dense smear layer (covering practically all dentinal tubules entrances). Statistical analysis was performed by Kruskal-Wallis and Bonferroni tests (p<0.05). Results: Overall, CUI and cervical thirds showed better RSL rates, compared with the other methods and thirds, respectively (p<0.05). More specifically, the cervical thirds showed better results in the CG, CI and PUI groups (p<0.05), whereas the cervical and middle thirds were not significantly different in the CUI group. Conclusion: CUI was the most effective method for the RSL.

The pitch of wire bond connections is decreasing to meet the need for higher interconnect densities, while at the same time, the ratio of wire length to diameter is increasing, which lowers the mechanical resonant frequency of the wire. In many applications in which MEMS sensors are coupled with ASIC front end electronics, the bonded wires can be subjected to a wide frequency spectrum of mechanical vibrations. One potential consequence is that the parasitic capacitances of the sensor could vary dynamically at a magnitude comparable to that of the sensor signal. In extreme cases, intermittent shorts or fatigue failures of the wire bonds could occur. A recent paper by Barber et. al, showed that wire bonds carrying alternating currents in a strong magnetic field could suffer fatigue failure.[1] Their analysis and experiments focused on simple loop geometries. In many applications, more complex wire bond geometries are used to minimize loop height and obtain dense wiring in stacked chip configurations. These geometries give rise to many more vibration modes with unique resonant frequencies and displaced shapes. We have used simple analytical beam models in conjunction with finite element models (FEM) to study various wire bond configurations subject to mechanical vibratory excitation. We focused on the effects of overall wire length and geometric shape on resonant modes. The finite element models were also used to calculate the capacitance between adjacent wires subject to mechanical excitation at one or more of their resonant frequencies. We show that there is an apparent shift in the time averaged capacitive coupling that increases with increasing vibration amplitude.

Sicyos angulatus may become an extremely dangerous invasive plant depending on its ability to naturalize and outcompete other species. To further understand the adaptive strategies of structures that could enhance its competitiveness, field surveys and experimental studies were conducted in plant communities where invasion may occur. The results showed that multistage branches of tendrils ensured that the plants could climb higher to strive for better photosynthetic opportunities. The single fleshy fruit of the infructescence was carpeted with slender white spines on which dense barbs were arranged, which could defend fruits against herbivores and contributed to long-distance dispersal. There would be beaklike lignified thorn forming to continue spreading when most of the barbed spines fell off during the fruit season. Rootstock was found in this herbaceous vine, which could accumulate various storage substances during development and helped the plants spread in harsh conditions. Moreover, the discovery of ants as new pollinators enriched the pollination system of S. angulatus, which greatly enhanced pollination efficiency. Above all, we conclude that this species has diverse adaptive strategies and a strong invasive capacity. It is urgent to find some way to slow or even stop its invasion effectively. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Previous observations indicated that the lamellipodium ("leading edge") of fibroblasts contains a dense meshwork, as well as numerous bundles (microspikes) of actin filaments. Most, if not all, of the filaments have a uniform polarity, with the "barbed" end associated with the membrane. I investigated whether and how actin subunits exchange in this region by microinjecting living gerbil fibroma cells (IMR-33) with actin that had been labeled with iodoacetamidotetramethylrhodamine. After incorporation of the labeled actin into the lamellipodium, I used a laser microbeam to photobleach a 3-4-micron region at and surrounding a microspike, without disrupting the integrity of the structure. I then recorded the pattern of fluorescence recovery and analyzed it using a combination of TV image intensification and digital image processing techniques. Fluorescence recovery was first detected near the edge of the cell and then moved toward the cell's center at a constant rate of 0.79 +/- 0.31 micron/min. When only part of the lamellipodium near the edge of the cell was photobleached, the bleached spot also moved toward the cell's center and through an area unbleached by the laser beam. These results indicated that steady state incorporation of actin subunits occurred predominantly at the membrane-associated end of actin filaments, and that actin subunits in the lamellipodium underwent a constant movement toward the center of the cell. I suggest that treadmilling, possibly in combination with other molecular interactions, may provide an effective mechanism for the movement of actin subunits and the protrusion of cytoplasm in the lamellipodium of fibroblasts.

Cynanchum rossicum (dog-strangling vine) and C. louiseae (black dog-strangling vine) are introduced, perennial herbs or small twining vines in the Milkweed family (Asclepiadaceae). Generic placement of these two species has been problematic, but are here treated as members of Cynanchum subgenus Vincetoxicum. The species occur primarily in natural upland areas, including understories of woodlands, pastures, old fields, shores, flood plains and ruderal areas of southern Ontario and Quebec and the northeastern United States. Ecosystems on well-drained, stony soils are often densely colonized, but both species can tolerate a wide moisture regime. Plants establish in full sun or under forest canopies and may form monospecific stands in all light conditions. They often form dense colonies which smother other vegetation and reduce invertebrate and vertebrate biodiversity. Reproduction is by polyembryonic, wind-dispersed seeds. Effective control is primarily by herbicides, since mechanical control is difficult and no biological control agents have been developed. A third European species, C. vincetoxicum, has been reported as an occasional garden escape in southern Ontario and the northeastern United States, but has not yet become naturalized. Information on this species is included because of its close relationship with C. rossicum and its better known biology. Key words: Dog-strangling vine, swallow-wort, Cynanchum, Vincetoxicum, CYKNI, invasive plant, weed biology

While the protrusive event of cell locomotion is thought to be driven by actin polymerization, the mechanism of forward translocation of the cell body is unclear. To elucidate the mechanism of cell body translocation, we analyzed the supramolecular organization of the actin–myosin II system and the dynamics of myosin II in fish epidermal keratocytes. In lamellipodia, long actin filaments formed dense networks with numerous free ends in a brushlike manner near the leading edge. Shorter actin filaments often formed T junctions with longer filaments in the brushlike area, suggesting that new filaments could be nucleated at sides of preexisting filaments or linked to them immediately after nucleation. The polarity of actin filaments was almost uniform, with barbed ends forward throughout most of the lamellipodia but mixed in arc-shaped filament bundles at the lamellipodial/cell body boundary. Myosin II formed discrete clusters of bipolar minifilaments in lamellipodia that increased in size and density towards the cell body boundary and colocalized with actin in boundary bundles. Time-lapse observation demonstrated that myosin clusters appeared in the lamellipodia and remained stationary with respect to the substratum in locomoting cells, but they exhibited retrograde flow in cells tethered in epithelioid colonies. Consequently, both in locomoting and stationary cells, myosin clusters approached the cell body boundary, where they became compressed and aligned, resulting in the formation of boundary bundles. In locomoting cells, the compression was associated with forward displacement of myosin features. These data are not consistent with either sarcomeric or polarized transport mechanisms of cell body translocation. We propose that the forward translocation of the cell body and retrograde flow in the lamellipodia are both driven by contraction of an actin–myosin network in the lamellipodial/cell body transition zone.

Abstract Mismatch repair deficient (MMRd) CRCs have high mutation/neo-antigen loads, leading to high immunogenicity and good immunotherapy response rates. We reasoned that the MMRd hypermutator phenotype should also promote intratumor heterogeneity (ITH) and evolvability; and investigated the genetic and immunological co-evolution in 69 regions from 6 localized and 13 metastatic MMRd CRCs by multi-region DNA-, RNA-sequencing and immunohistochemistry. All tumors had high truncal mutation loads (median: 44 mutations in 191 sequenced genes; whole exome equivalent: 1870 mutations). A median of 16.1% mutations per region were heterogeneous, indicating pervasive ITH. Phylogenetic analyses showed that metastases had diverged before subclonal diversification of the primary tumor in 75% of assessable cases. Thus, the ability to metastasize was frequently acquired early during tumor evolution. Driver aberrations evolved with a clear hierarchy: those in the WNT and RTK-MAPK pathways and in TGFbR-family members were almost always truncal (87.0%, 86.4% and 83.7%), indicating a critical role for cancer initiation. In contrast, genetic aberrations that are known to confer immune evasion (IE) were predominantly subclonal (71.4%) and parallel evolution of IE drivers occurred in 4/6 tumors that harboured any subclonal IE driver. This substantiates immune selection pressure as the main driver of Darwinian evolution during tumor progression. These IE drivers are known to confer resistance to checkpoint-inhibitor immunotherapy. ITH therefore needs to be addressed for predictive biomarker development. We quantified CD8 T-cell infiltrates as a surrogate measure of tumor immunogenicity; distinguishing tumors with low CD8 T-cell infiltrates (mean: 3.9% T-cells of all nucleated cells) and those with high infiltrates (mean: 12.2%). T-cell infiltrates showed high ITH in the latter group. This suggested a tumor-intrinsic setpoint, accompanied by marked variability in tumors with dense infiltrates. T-cell densities did not correlate with truncal mutation loads or heterogeneity metrics, questioning how immunogenicity is regulated. Phylogenetic analysis defined three patterns of IE evolution: tumors with subclonal, with pan-tumor, or without any identifiable IE drivers. CD8 T-cell abundance was highest in tumors with subclonal IE, supporting selection pressure from high CD8 T-cell infiltrates as the proximate cause for IE evolution. Tumors with pan-tumor IE showed low CD8 T-cell infiltrates. Surprisingly, tumors without IE drivers had the lowest CD8 T-cell abundance, indicating an alternative mechanism of immune escape. Low densities of CD8 T-cells at the tumor margin and low expression of T-cell chemo-attractants suggested impaired T-cell recruitment in these. Together, we show that immune recognition is a major driver of Darwinian evolution in MMRd CRCs and that immune infiltrates and IE drivers co-evolve interdependently. Whether sensitivity to checkpoint-inhibitor immunotherapy differs between the three phylogenetic MMRd CRC subtypes needs to be assessed in clinical trials. Citation Format: Benjamin R. Challoner, Andrew Woolston, David Lau, Marta Buzzetti, Louise J. Barber, Tom Lund, Harold B. Sansano, Katharina von Loga, Héctor Lázare-Iglesias, Ruwaida Begum, Richard Crux, David Cunningham, Ian Chau, Naureen Starling, Juan Ruiz-Bañobre, Tony Dhillon, Marco Gerlinger. Genetic and immune landscape evolution defines subtypes of MMR deficient colorectal cancer [abstract]. In: Proceedings of the AACR Special Conference on the Evolutionary Dynamics in Carcinogenesis and Response to Therapy; 2022 Mar 14-17. Philadelphia (PA): AACR; Cancer Res 2022;82(10 Suppl):Abstract nr PR012.

The aim of this research was to investigate the students’ ability to create classification criteria based on reflections on different types of skills related to a classification. This research included 103 students of three public schools in the Metropolitan Region of Recife organized into three groups: G1 - participated in a sequence of activities that involved the understanding of the descriptor/criterion; G2 - participated in a sequence of activities that involved re?ection on element, class and descriptor/criterion; G3 - did not participate in sequences of activities, constituting as a control group. It was proposed a pre-test, a sequence of teaching activities (groups G1 and G2) and a post-test with each group. The results showed that the students of the three groups presented a poor performance in the pre-test, showing difficulties in creating criteria to classify freely. However, after the children’s experiences in the sequences of activities, significant advances were observed in the learning of groups G1 and G2 and not in G3. Thus, it is evident the possibility of students learning to create classification criteria, regardless of the type of intervention. Thus, we can say that students of the early years, when stimulated to re?ect on how to classify, demonstrate ability and facility to learn and understand that there is a diversity of criteria that can be used as long as they meet the properties of exclusivity and completeness.ResumoEssa pesquisa teve como objetivo investigar a aprendizagem de alunos do 4º ano do Ensino Fundamental sobre a habilidade de criar critérios de classificação a partir de reflexões sobre diferentes tipos de habilidades relacionadas à classificação. Participaram do estudo 103 alunos de três escolas públicas da Região Metropolitana do Recife organizados em três grupos: G1 – participava de uma sequência de atividades que envolvia a compreensão do descritor/critério; G2 – participava de uma sequência de atividades que envolvia a re?exão sobre elemento, classe e descritor/critério; G3 – não participava de sequências de atividades, se constituindo como grupo controle. Foram realizados um pré-teste, uma sequência de atividades de ensino (grupos G1 e G2) e um pós-teste com cada grupo. Os resultados revelaram que os alunos dos três grupos apresentaram um fraco desempenho no pré-teste, demonstrando dificuldades em criar critérios para classificar livremente. Entretanto, após as vivências nas sequências de atividades foram observados avanços significativos na aprendizagem dos grupos G1 e G2 e não no G3. Dessa forma, fica evidente a possibilidade de aprendizagem dos alunos em criar critérios de classificação, independente do tipo de intervenção. Assim, podemos afirmar que alunos dos anos iniciais quando levados a re?etir sobre como classificar demonstram capacidade e facilidade para aprender e compreender que há uma diversidade de critérios que podem ser utilizados desde que atendam às propriedades de exclusividade e exaustividade.Keywords: Statistics, Classification, Learning, Primary school.Palavras-chave: Estatística, Classificação, Ensino aprendizagem, Anos Iniciais.ReferencesBARRETO, Monik; GUIMARÃES, Gilda. Estratégias utilizadas por crianças da educação infantil para classificar. EM TEIA – Revista de Educação Matemática e Tecnológica Iberoamericana, vol. 7, número 1, 2016.BIVAR, Dayse; SELVA, Ana. Analisando atividades envolvendo gráficos e tabelas nos livros didáticos de matemática. In: XIII CIAEM, Anais..., Recife, 2011.CRUZ, Edneri. Classificação na Educação Infantil: o que propõem os livros e como é abordada por professores. 2013. 170f. Dissertação de Mestrado em Educação Matemática e Tecnológica – Edumatec, Centro de Educação, Universidade Federal de Pernambuco, Recife, 2013.GITIRANA, Veronica. Classificação e Categorização. Pacto Nacional pela Alfabetização na Idade Certa: Educação Estatística. Ministério da Educação, Secretaria de Educação Básica, Diretoria de Apoio à Gestão Educacional. Caderno 7. 2014.GITIRANA, Veronica; CASTELO-BRANCO, Walquíria. Categorizar: habilidade necessária à formação básica. TV Escola/Salto para o futuro. Rio de Janeiro, n. 24, set. 2014.GUIMARÃES, Gilda; Gitirana, Veronica. Classificações: o que sabem os alunos de 3a série do ensino fundamental. XVI Encontro de Pesquisa Educacional do Norte e Nordeste – EPENN, Anais..., Aracajú – SE, 2003.GUIMARÃES, Gilda; OLIVEIRA, Izabella. Do future primary school teachers know how to classify? XXXVIII Conference of the International Group for the Psychology of Mathematics Education and XXXVI The North American Chapter of the Psychology of Mathematics Education. Proceeding... Vancouver, Canadá, July, 2014. p.1IONESCU, Thea. Object categorization in the preschool years and its relation with cognitive inhibition. XXVII Annual Conference of the Cognitive Science Society. July, p. 21-23, Stresa, Italy, 2005.LEITE, Marcela; CABRAL, Paula; GUIMARÃES, Gilda; LUZ, Patrícia. O Ensino de Classificação e o Uso de Tabelas. Caderno de Trabalhos de Conclusão de Curso de Pedagogia. Recife, UFPE, 2013.LINS, Walquíria. Procedimentos lógicos de classificação através de um banco de dados: um estudo de caso. 2000. 123f. Monografia (Curso de Especialização em Informática na Educação) – UFPE, Recife, 2000.LUZ, Patrícia. Classificações nos anos iniciais do Ensino Fundamental: o papel das representações. 2011. 113p. Dissertação (Mestrado em Educação Matemática e Tecnológica) – Edumatec, Centro de Educação, Universidade Federal de Pernambuco, 2011.MARESCHAL, Denis; QUINN, Paul. Categorization in infancy. TRENDS in Cognitive Sciences. Vol.5, Nº.10, p.443-450, October, 2001.MONTEIRO, Carlos; SELVA, Ana. Investigando atividades de interpretação de gráficos entre professores do Ensino Fundamental. In: 24° Reunião Anual da Associação Nacional De Pós-Graduação e Pesquisa em Educação - ANPED, Anais... Caxambu. 2001.PAGAN, Adriana; MAGINA, Sandra. O ensino de estatística a partir da interdisciplinaridade: um estudo comparativo. In: 10º encontro Nacional de Educação Matemática – ENEM, Anais..., Salvador, 2010.PIAGET, Jean; INHELDER, Barbel. Gênese das Estruturas Lógicas Elementares. Rio de Janeiro: Zahar, 1983.SILVA, Edilza. Como são propostas pesquisas em livros didáticos de Ciências e Matemática dos Anos Iniciais do Ensino Fundamental. 2013. 133f. Dissertação (Mestrado em Educação Matemática e Tecnológica) – Edumatec, Centro de Educação, Universidade Federal de Pernambuco, Recife, 2013.VERGNAUD, Gerard. El niño, las Matemáticas y la realidad: problemas de la enseñanza de las Matemáticas en la escuela primaria. México: Trillas, 1991.VIELLARD, Sandrine; GUIDETTI, Michèle. Children's perception and understanding of (dis) similarities among dynamic bodily/facial expressions of happiness, pleasure, anger, and irritation. Journal of Experimental Child Psychology, EUA, v. 102, p. 78-95, 2009.

Introducción: El cáncer de próstata (CP) el segundo cáncer diagnosticado en hombres, con mayor incidencia a los 66 años. La obesidad, el tabaquismo, alcoholismo y antecedentes familiares de CP se han encontrado asociados al riesgo de metástasis. El objetivo del presente estudio fue medir la asociación entre factores y el estado metastásico en pacientes con CP en un centro único de referencia en Ecuador. Metodología: El presente estudio analítico, se realizó en el Hospital “Teodoro Maldonado Carbo”, en Guayaquil-Ecuador, en el período enero-diciembre del 2019. El cálculo muestral fue no probabilístico, tipo censo. Se incluyeron casos con CP. Las variables fueron: edad, PSA, escala de Gleason, presencia de metástasis, sintomatología, tabaquismo, obesidad y antecedentes. Se presenta Odds Ratio como medida de asociación con intervalo de confianza del 95% y valor P. Resultados: El estudio incluyó 363 pacientes, con edad promedio de 75.2 ± 9.6 años. El grupo con metástasis fue de 202 casos (55.65%). Metástasis ósea 32.5%, pulmonar 9.6%, ganglionar 8.8% y hepático 4.75%. En la sintomatología la más frecuente fue, disuria (44.4%); el 33.6% con polaquiuria, un 13.2% hematuria y 8.8% tenesmo. El estadio Gleason-9 OR=24.85 (IC 95% 1.47-419.8) P=0.0259. El nivel de PSA >19 ng/ml OR= 6.996 (IC 95% 2.68-18.29) P=0.0001. El tabaquismo OR=2.34 (IC 95% 1.52-3.60) P=0.0001. Fueron factores protectores el valor de PSA <19 ng/ml OR=0.082 (IC 95% 0.043-0.157) P<0.0001, acudir a consulta de Hipertensión arterial OR=0.33 (IC 95% 0.161-0.691) P=0.0032 y el estadío Gleason-6 OR=0.108 (IC 95% 0.0665-9.1736) P<0.0001. Conclusión: Los niveles de PSA >19 ng/ml y el estadio Gleason >9 se asocian a la presencia de metástasis en pacientes con CP. Recibido: Julio 14, 2022 Aceptado: Octubre 27, 2022 Publicado: Diciembre 2, 2022 Editor: Dra. Evelyn Valencia Espinoza. Introducción El cáncer de próstata es el segundo cáncer más diagnosticado en hombres alrededor del mundo, con mayor incidencia promedio a los 66 años [1]. En países desarrollados, el cáncer de próstata se diagnostica a edades más tempranas cuando este se confina a la glándula prostática, debido al uso del antígeno prostático específico (PSA) [2]. El antígeno prostático específico es proteína creada por el epitelio prostático, el cual, es específico de órgano, más no de cáncer, debido a que el mismo puede estar elevado en patologías como prostatitis, hiperplasia prostática benigna, entre otras. Sin embargo, es el mejor biomarcador hasta ahora para el diagnóstico temprano del cáncer de próstata. Según la American Academy of Family Physicians, se ha determinado que 3 de cada 10 hombres con elevado PSA, tienen un alto riesgo de sufrir de cáncer de próstata que va a depender de la edad y el nivel del PSA que se encuentre. Algunos estudios demuestran que las pruebas de rutina de PSA en hombres entre 55 a 66 años disminuyen la mortalidad por cáncer de próstata, de manera significativa. Sin embargo, la baja especificidad para Cáncer de Próstata de la prueba, hace que la interpretación de ella no sea concluyente [3]. La Escala de Gleason es un sistema de graduación que sirve, una vez establecido el diagnóstico de adenocarcinoma de próstata, para medir la agresividad histopatológica de la neoplasia [4]. Está plenamente establecido que la escala de Gleason, de acuerdo a los hallazgos histopatológicos del tumor, nos permite categorizar a los pacientes de una manera más precisa y de esta manera tener una idea clara del pronóstico de dicho paciente. Con estos antecedentes se estableció el objetivo de determinar los factores de riesgo asociados a metástasis en pacientes con cáncer de próstata en un centro de referencia regional en Guayaquil-Ecuador. Materiales y métodos Diseño del estudio El presente estudio es observacional-analítica. La fuente es retrospectiva. Área de estudio El estudio se realizó en el servicio de urología del Hospital de Especialidades “Teodoro Maldonado Carbo”, del Instituto Ecuatoriano de Seguridad Social, en Guayaquil-Ecuador. El período de estudio fue del 1ro de Enero del 2019 hasta el 31 de diciembre del 2019. Universo y muestra El universo fue conformado por todos los pacientes registrados en la institución. El cálculo del tamaño muestral fue no probabilístico, tipo censo, en donde se incluyeron todos los casos incidentes en el período de estudio. Participantes Se incluyeron casos de pacientes adultos diagnosticados con cáncer de próstata en quienes se contó con valoración de PSA y la escala de Gleason. Se excluyeron registros incompletos para el análisis. Variables Las variables fueron edad, PSA, escala de Gleason, presencia de metástasis, sintomatología, métodos diagnósticos. Procedimientos, técnicas e instrumentos. Los datos fueron recogidos de la historia clínica en un formulario diseñado exclusivamente para tal propósito. Los diagnósticos de CP se realizaron por punción aspiración por aguja fina (PAAF) guiada por ecografía. Las placas fueron leídas por Patólogos distintos. Se usó el sistema AS400 para la pesquisa de casos usando los diagnósticos codificados: C61. Evitación de sesgos Para garantizar la confiabilidad de la información los investigadores fueron entrenados sobre la recolección de los datos. Se usó una lista de doble chequeo para incluir los casos. Los datos fueron validados y curados por el investigador principal. Análisis estadístico Recopilada la información en una hoja electrónica Excel, se ingresó en una matriz de datos del software SPSS™ 22.0 (IBM, Chicago, USA). Se utilizó estadística descriptiva en base a frecuencias y porcentajes para las variables cualitativas y para las cuantitativas medidas de tendencia central. Se presenta Odds Ratio como medida de asociación con intervalo de confianza del 95% y valor P. Resultados El estudio incluyó 363 pacientes. Caracterización clínica Fueron 363 pacientes, con edad promedio de 75.2 ± 9.6 años. La edad mínima fue de 49 años, y la máxima de 98 años. Los pacientes con metástasis fueron 202 casos (55.65%). Con respecto al lugar de diseminación metastásica, se encontró mayor predilección a desarrollar metástasis ósea en el 32.5% de los pacientes, seguido de metástasis pulmonar con el 9.6%, metástasis ganglionar con el 8.8% y a nivel hepático 4.75% de los casos. En la sintomatología la más frecuente fue, disuria con el 44.4%; el 33.6% con polaquiuria, un 13.2% hematuria y 8.8% tenesmo. El estudio más frecuente utilizado, fue el eco prostático, con un 42.7%; la tomografía 20.4%), gammagrafía prostática 25.6%, la resonancia magnética con el 11.3%. Factores clínicos El 44.4% de los pacientes padecía alcoholismo, el 27.5% presentaba tabaquismo, el 23.1% eran consumidores de carnes y lácteos; otros hábitos poco frecuentes se relacionaron con el 4.7%; 0.3% no refería ningún hábito de importancia. Dentro de los antecedentes patológicos se evidenció que, 54.5% de los pacientes tenían antecedentes familiares de Cáncer de próstata, 29.2% presentaba obesidad y sobrepeso, en tercer lugar, con 9.9% de los pacientes padecía de hipertensión arterial y entre otros antecedentes asociados tenían un porcentaje del 5.5% (Tabla 1). De la totalidad de los evaluados, el 45.2% tuvieron Gleason 6, mientras que aquellos con valores en la Escala de Gleason de 10, alcanzaban el 1.4%. La medición del riesgo se presenta en la tabla 1. Se constituyeron factores de riesgo para el desarrollo de metástasis el tabaquismo, y el estadío clínico Gleason 7, 8 y 9, los valores de PSA en rangos de 19-49 ng/ml, de 50 a 99 ng/ml y 100-299 ng/ml. Se establecieron como factores de protección estadísticamente significativos al antecedente personal de Hiperplasia prostática benigna, a la condición de hipertensión arterial, al estadio clínico Gleason 6, al valor de PSA <19 ng/ml (Tabla 1). Discusión Al comparar el presente estudio con investigaciones previas se constató que la edad constituye un factor de riesgo no modificable para padecer cáncer prostático, según el Instituto Nacional de Cáncer en Estados Unidos, en su programa estadístico de Vigilancia, Epidemiología y Resultados (SEER) entre los años 2015-2019 se encontró una mayor incidencia de casos en los pacientes que tenían entre 70-74 años de edad [5], así también lo describió el trabajo de Leitzmann et al, en los años correspondientes a 2000-2008 donde la incidencia comienza a aumentar desde los 40-44 años de edad para llegar a su pico máximo de 984.8 pacientes por cada 100.000 hombres con edades entre 70-74 años [6], similarmente en este estudio el mínimo de edad fue 49 años en la población con un máximo de 98 y un media de 75.15 respectivamente. En cuanto a la distribución de regiones anatómicas donde se suele presentar la metástasis en el cáncer prostático; en primer lugar se determinó a los huesos (84%) en segundo lugar a nódulos linfáticos distantes (10.6%) y hepático (10.2%) [7] según un estudio conducido por Gandaglia et al. lo cual en similitud a este estudio el sitio más frecuente fue a nivel óseo con un 32.5% de todos los pacientes, seguido de la metástasis pulmonar con 9.6% y ganglionar 8.8%. A su vez Auz y Brito (2018) encontraron en su investigación realizada en el Hospital Solca Núcleo de Quito-Ecuador que incluyó a 1713 pacientes donde el sitio más frecuente de metástasis fue el óseo en un 82.25%, seguido del ganglionar linfático con 7.05% y luego 2.8% a nivel pulmonar [8]. En lo que corresponde a los hábitos de los pacientes, un 44.4% de pacientes en este estudio consumía de alcohol regularmente, mientras que el 27.5% consumía tabaco y el 23.1% eran consumidores de carnes y lácteos, lo cual se contrasta con el estudio de Auz y Brito (2018) detallado anteriormente donde 31.1% de los pacientes consumían alcohol regularmente y el 28.3% de los pacientes consumían tabaco al momento de diagnóstico, no se midieron datos en relación al consumo de lácteos y carnes rojas [8]. Un factor de riesgo no modificable muy importante fue la presencia de antecedentes familiares de cáncer de próstata en este estudio con un 54.5% de los pacientes lo cual en el trabajo de Auz y Brito (2018) solamente 9% tuvieron antecedentes familiares de cáncer prostático y un 23.2% de otros tipos de cánceres [23]. Según Barber et al. en su estudio se identificó que pacientes con historia familiar de cáncer prostático únicamente, tienen un riesgo aumentado del 68% de padecer lo mismo (95% CI 1.53-1.83) y un 72% de enfermedad letal [9]. Así también lo describió Powell IJ (2011) en su publicación que dice que hombres con relativos de primer grado (Padre, hermano, hijo) tienen un riesgo de desarrollar cáncer prostático que es aproximadamente del doble a la población general [10]. En el presente estudio 29.2% de los pacientes presentaba sobrepeso y 9.9% de los pacientes padecía hipertensión arterial, así mismo en el estudio de Möller et al. se encontró que un IMC alto (26) en comparación a 20-22 a la edad de 21 años fue asociada con riesgo menor de cáncer prostático letal o avanzado y Gleason 7 [11], también se habla sobre que de los componentes del síndrome metabólico incluyendo la hipertensión arterial ninguno tiene relación con el desarrollo de cáncer prostático [10]. Dentro de los síntomas más frecuentes en esta investigación se encontraron la disuria con un 44.4% de todos los pacientes, le siguió la polaquiuria (33.6%) y finalmente la hematuria (13.2%). De manera similar el estudio de Birtle et al. llevado a cabo entre los años 2000-2001 con bases de datos de la Asociación Británica de Cirujano Urológicos determinó que de 33 pacientes con cáncer metastásico de próstata con PSA <10 ng/ml 51% se presentó con síntomas urinarios y/o dolor pélvico, 21% presentó dolor óseo, 18% retención urinaria y 10% caquexia y malasia [12]. En el presente estudio de la totalidad de pacientes, 45.2% de ellos tuvieron puntajes de Gleason de 6 y en menor medida con 1.4% de los pacientes puntajes de Gleason 10, esto se puede comparar con el estudio de Thomsen et al. donde se encontró que altos puntajes en la escala de Gleason se relacionaron a la presencia de metástasis, así en ese estudio los pacientes que presentaron metástasis a distancia según la escala TNM (M1), 41% tenía un puntaje de Gleason 5, mientras que solo el 3% tenía Gleason 1 [13]. El método de imagen más utilizado en este estudio fue el de la ecografía prostática con un 42.7% y en menor medida la resonancia magnética con el 11.3%. En la investigación de Huang et al. se encontró que el uso de la Resonancia Magnética con el sistema PI-RADS v2 fue el sistema más preciso para predecir la metástasis a nódulos linfáticos en la zona pélvica, los pacientes con PI-RADs <5 fueron asociados con un riesgo muy bajo de metástasis ganglionar [14]. La ecografía prostática 3D se demostró un 84% de sensibilidad y 96% de especificidad para identificar extensión tumoral extra capsular macroscópica y fue capaz de identificar a 14/16 diseminaciones a la vesícula seminal según Mitterberger et al. en el año 2008 [15]. Finalmente con respecto a valoraciones de PSA que se presentaron en los pacientes diagnosticados con cáncer de próstata, se pudo evidenciar, que el 100% de los pacientes con PSA mayor de 500 ng/ml tuvo metástasis, así como el grupo de pacientes con 300-499 ng/ml PSA, a diferencia del grupo con menos de 19 ng/ml PSA donde solo el 40.6% tuvo metástasis, lo cual se puede comparar al estudio de Thomsen et al. donde de los pacientes con más de 400 ng/ml, el 64.9% presentó metástasis, le sigue el grupo con valores de 200-399 ng/ml donde el 54% desarrolló metástasis y al final el grupo con menos de 19 ng/ml tuvo solo al 1.45% de pacientes con metástasis, podemos así deducir que el valor de PSA es directamente proporcional al riesgo de metástasis, sin embargo, recomendamos tomar en cuenta más factores de riesgos de manera conjunta para una evaluación integral del paciente [13]. Conclusiones En orden de importancia estadística los factores asociados a metástasis en cáncer prostático son el estadio clínico Gleason 9 y 8, Los niveles de PSA entre 50 a 299 ng/ml, y el tabaquismo. Fueron factores protectivos el antecedente clínico de Hiperplasia Prostática benigna, hipertensión y el nivel de PSA <19 ng/ml. Nota del Editor La Revista Oncología Ecu permanece neutral con respecto a los reclamos jurisdiccionales en mapas publicados y afiliaciones institucionales. Agradecimientos Los autores agradecen a todas las personas de la Institución que colaboraron en el desarrollo de esta investigación. Información administrativa Abreviaturas APP: antecedentes patológicos personales. APF: Antecedentes patológicos familiares. HPB: hiperplasia prostática benigna. Ca: Cáncer. PSA: Antígeno prostático específico. OR: Odds ratio. IC: intervalo de confianza Archivos Adicionales Ninguno declarado por los autores. Fondos Los autores no recibieron ningún tipo de reconocimiento económico por este trabajo de investigación. Disponibilidad de datos y materiales Existe la disponibilidad de datos bajo solicitud al autor de correspondencia. No se reportan otros materiales. Contribuciones de los autores Emily Gabriela Chimbo Acuña: conceptualización, validación, visualización, metodología, administración de proyecto, escritura: revisión y edición. Karen Gabriela Valverde Zambrano: conceptualización, curación de datos, análisis formal, adquisición de fondos, investigación, recursos, software, redacción - borrador original. Iván Altamirano: conceptualización, curación de datos, análisis formal, adquisición de fondos, investigación, recursos, software. Todos los autores leyeron y aprobaron la versión final del manuscrito. Aprobación del comité de ética No aplica a estudios de bases de datos o historias clínicas. Consentimiento para publicación El presente estudio es un análisis de base de datos, no aplica para este tipo de estudio. Referencias Professionals S-O. EAU Guidelines: Prostate Cancer [Internet]. [citado 20 de octubre de 2021]. Disponible en: https://uroweb.org/guideline/prostate-cancer/#note_11 Barry MJ, Nelson JB. Patients Present with More Advanced Prostate Cancer since the USPSTF Screening Recommendations. J Urol. diciembre de 2015;194(6):1534-6. DOI: 1016/j.juro.2015.09.033 PMid: 26384450 Catalona WJ, Richie JP, Ahmann FR, Hudson MA, Scardino PT, Flanigan RC, et al. Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: results of a multicenter clinical trial of 6,630 men. J Urol. mayo de 1994;151(5):1283-90. DOI: 1016/S0022-5347(17)35233-3 PMid: 7512659 Schröder FH, Hugosson J, Roobol MJ, Tammela TL, Ciatto S, Nelen V, Kwiatkowski M, Lujan M, Lilja H, Zappa M, Denis LJ, Recker F, Berenguer A, Määttänen L, Bangma CH, Aus G, Villers A, Rebillard X, van der Kwast T, Blijenberg BG, Moss SM, de Koning HJ, Auvinen A; ERSPC Investigators. Screening and prostate-cancer mortality in a randomized European study. N Engl J Med. 2009 Mar 26;360(13):1320-8. DOI: 10.1056/NEJMoa0810084. Epub 2009 Mar 18. PMID: 19297566. Surveillance, Epidemiology, and End Results Program (SEER) of the National Cancer Institute. Fast Stats: An interactive tool for access to SEER cancer statistics. Bethesda, MD: SEER, National Cancer Institute; nd. Available from: www.seer.cancer.gov/faststats. Leitzmann MF, Rohrmann S. Risk factors for the onset of prostatic cancer: age, location, and behavioral correlates. Clin Epidemiol. 5 de enero de 2012;4:1 DOI: 2147/CLEP.S16747 PMid: 22291478 PMCid: PMC3490374 Gandaglia G, Abdollah F, Schiffmann J, Trudeau V, Shariat SF, Kim SP, et al. Distribution of metastatic sites in patients with prostate cancer: A population-based analysis. Prostate. febrero de 2014;74(2):210-6. DOI: 1002/pros.22742. PMid:24132735 Auz Fierro E, Brito Chasiluisa H. Factores relacionados con la supervivencia de pacientes con cancer de prostata en el hospital solca nucleo de quito durante el periodo 2003 - 2018. [Internet]. [Hospital Solca Nucleo de Quito]: Pontificia Universidad Católica del Ecuador; 2018. Disponible en: 22000 Barber L, Gerke T, Markt SC, Peisch SF, Wilson KM, Ahearn T, et al. Family History of Breast or Prostate Cancer and Prostate Cancer Risk. Clinical Cancer Research. 1 de diciembre de 2018;24(23):5910-7. DOI: 1158/1078-0432.CCR-18-0370. PMid: 30082473 PMCid: PMC6279573 Powell IJ. The precise role of ethnicity and family history on aggressive prostate cancer: a review analysis. Arch Esp Urol. 2011 Oct;64(8):711-9. PMID: 22052754; PMCID: PMC3859428. Möller E, Wilson KM, Batista JL, Mucci LA, Bälter K, Giovannucci E. Body size across the life course and prostate cancer in the Health Professionals Follow-up Study: Body size and prostate cancer. Int J Cancer. 15 de febrero de 2016;138(4):853-65. DOI: 1002/ijc.29842 PMid: 26355806 PMCid: PMC5042346 Birtle AJ, Freeman A, Masters JRW, Payne HA, Harland SJ, BAUS Section of Oncology Cancer Registry. Clinical features of patients who present with metastatic prostate carcinoma and serum prostate-specific antigen (PSA) levels < 10 ng/mL: the «PSA negative» patients. Cancer. 1 de diciembre de 2003;98(11):2362-7. DOI: 1002/cncr.11821 PMid: 14635070 Thomsen FB, Westerberg M, Garmo H, Robinson D, Holmberg L, Ulmert HD, et al. Prediction of metastatic prostate cancer by prostate-specific antigen in combination with T stage and Gleason Grade: Nationwide, population-based register study. PLoS One. 2020;15(1):e0228447. DOI: 1371/journal.pone.0228447 PMid: 31995611 PMCid: PMC6988964 Huang C, Song G, Wang H, Lin Z, Wang H, Ji G, et al. Preoperative PI-RADS Version 2 scores helps improve accuracy of clinical nomograms for predicting pelvic lymph node metastasis at radical prostatectomy. Prostate Cancer Prostatic Dis. marzo de 2020;23(1):116-26 DOI: 1038/s41391-019-0164-z PMid: 31383954 Mitterberger M, Pinggera GM, Pallwein L, Gradl J, Frauscher F, Bartsch G, et al. The value of three-dimensional transrectal ultrasonography in staging prostate cancer. BJU Int. julio de 2007;100(1):47-50. DOI: 1111/j.1464-410X.2007.06845.x PMid: 17433033.

Sir Ernst Gombrich and the Barber from TuscanyIn the spirit of Sir Ernst Gombrich, this essay uses an anecdote—a chat between Gombrich and a barber from Tuscany—to illustrate a deeper point, namely, how cultural memory, tradition, and a canon give rise to an implied language of culture and cultural value. Gombrich staunchly defended tradition against relativism. By relativism, he meant something like "radical subjectivism." To his mind, subjectivism (in the cultural and social sense of the term) is not only impossible, since meaning is conferred through culture and society, but it is also dangerous (in the cultural and ethical sense of the term), since it denies the existence of shared values. Against consensus on the one hand and radical subjectivism on the other, Gombrich advocated a middle way: criticism and self-criticism to ensure latitude; the search for "truth" to ensure a limited plurality of interpretations.

Prickly pear cactus is not a problem in every pasture in Florida, but where it is found, it is often the biggest and most difficult issue to manage: mowing prickly pear fragments the pads and dramatically increases the infestation, and although prickly pear doesn’t form dense canopies and doesn’t outcompete desirable forage grasses, its impact on grazing can be just as severe because of its barbed quills. This 2-page fact sheet was written by J. A. Ferrell and B. A. Sellers, and published by the UF Department of Agronomy, September 2011. SS-AGR-354/AG363: Prickly Pear Cactus Control in Pastures (ufl.edu)

AbstractPhysical interactions of cells with the underlying extracellular matrix (ECM) play key roles in multiple cellular processes. The actin cytoskeleton is a central driver and regulator of cellular dynamics, that produces membrane-protrusions such as lamellipodia and filopodia. Here, we examined actin organization in expanding lamellipodia during early stages of cell spreading. To gain insight into the 3D actin organization, we plated fibroblasts on galectin-8 coated EM grids, an ECM protein presents in disease states. We then combined cryo-electron tomography with advanced image processing tools for reconstructing the structure of F-actin in the lamellipodia. This approach enabled us to resolve the polarity and orientation of filaments, and the structure of the Arp2/3 complexes associated with F-actin branches. We show that F-actin in lamellipodial protrusions forms a dense network with three distinct sub-domains. One consists primarily of radial filaments, with their barbed ends pointing towards the membrane, the other is enriched with parallel filaments that run between the radial fibers, in addition to an intermediate sub-domain. Surprisingly, a minor, yet significant (~10%) population of actin filaments, are oriented with their barbed-ends towards the cell center. Our results provide structural insights into F-actin assembly and dynamic reorganization in the leading edge of spreading cells.

Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin cytoskeleton plays a key role in the formation and modification of spine morphology, however many of the underlying regulatory mechanisms remain unclear. Capping protein (CP) is a major actin regulating protein that caps the barbed ends of actin filaments, and promotes the formation of dense branched actin networks. Knockdown of CP impairs the formation of mature spines, leading to an increase in the number of filopodia-like protrusions and defects in synaptic transmission. Here, we show that CP promotes the stabilization of dendritic protrusions, leading to the formation of stable mature spines. However, the localization and function of CP in dendritic spines requires interactions with proteins containing a capping protein interaction (CPI) motif. We found that the CPI motif-containing protein Twinfilin-1 (Twf1) also localizes to spines where it plays a role in CP spine enrichment. The knockdown of Twf1 leads to an increase in the density of filopodia-like protrusions and a decrease in the stability of dendritic protrusions, similar to CP knockdown. Finally, we show that CP directly interacts with Shank and regulates its spine accumulation. These results suggest that spatiotemporal regulation of CP in spines not only controls the actin dynamics underlying the formation of stable postsynaptic spine structures, but also plays an important role in the assembly of the postsynaptic apparatus underlying synaptic function.

SinopseNo 13º andar do Centro de Filosofia e Ciências Humanas, uma intervenção que sinaliza para elementos de tensão do fazer antropológico e museológico hoje no Brasil: a exposição Agnosis. Apesar de ser comemorativa, celebrando os 10 anos de criação do Curso de Museologia da UFPE, a efervescência característica do momento de surgimento do curso encontra hoje um contraponto não tão otimista.Fazendo uso de recursos narrativos diversos, a exposição comunica pela ausência, por metáforas e também pelo incômodo que transmite à sensibilidade. No dia-a-dia das aulas e da pesquisa, é preciso tomar cuidado com um arame farpado que vai do chão ao teto, em torno de uma das pilastras do andar.A exposição Agnosis parte de uma releitura da exposição “Tensões e Diálogos”, realizada em 2010 com a curadoria de Antonio Motta, Denis Bernardes e Solange Coutinho. Nas palavras da curadoria, Agnosis “convida o espectador, o visitante, a desvendar e recobrar sentidos que aparentemente se ocultam na natureza das coisas [...] porém, o efeito do real e sua inteligibilidade nunca serão completamente evidenciados”. Assim sendo, o ensaio aqui apresentado é uma narrativa sobre uma narrativa sobre outra narrativa... camadas discursivas recobrindo ideias e acontecimentos. A insistência em ainda assim construir conhecimento e representações é a indicação de um caminho.Palavras-chave: exposição; narrativa; conhecimento; agnosis.Fotografia e edição de imagens: Maíra Acioli Ficha técnica da exposiçãoExercício experimental de curadoria compartilhada, projeto e narrativa expográfica, exigido como cumprimento de finalização da disciplina Expografia I e II (2018), da graduação em Museologia da UFPE, sob a supervisão do professor Antonio Motta. Curadoria/Projeto Expográfico/Produção/MontagemBárbara Gondim Bezerra SilvaFabrício Tavares de Arruda dos SantosFrancisca Juscizete Queiroz de LimaManoel Francisco da Silva NetoStella Victoria Arcelino Bastos Lavra Design GráficoOlívia Morim IluminaçãoCamila Maria Silva de Moraes Santos AgradecimentosRevisão de texto: Prof. Lepê Correia RealizaçãoDepartamento de Antropologia e Museologia | DAMChefia Ana Cláudia Rodrigues da SilvaCurso de Bacharelado em MuseologiaCoordenador Alexandro Silva de JesusDisciplina de Expografia I e II (2018)Prof. Antonio Motta Sinopsis On the 13th floor of the Center for Philosophy and Human Sciences, an intervention that points to tension elements about anthropological and museological practice today in Brazil: the Agnosis exhibition. Despite being commemorative, celebrating the 10th anniversary of the creation of the UFPE Museology Course, the effervescence characteristic concerning the course’s moment of emergence finds, today, a not so optimistic counterpoint.Using diverse narrative resources, the exposition communicates by its absence, by metaphors and also by the discomfort that it transmits to sensibility. In our day-to-day classes and research, we need to be careful about the floor-to-ceiling barbed wire around one of the floor pilasters. The Agnosis exhibition begins with a rereading of the exhibition “Tensions and Dialogues”, curated in 2010 by Antonio Motta, Denis Bernardes and Solange Coutinho. In the words of the curators, Agnosis “invites the viewer, the visitor, to unravel and recover senses that seemingly lurk in the nature of things [...] however, the effect of the real and its intelligibility will never be fully evident”. Therefore, the essay presented here is a narrative about a narrative over another narrative... discursive layers covering ideas and events. The insistence on still building knowledge and representations is an indication of a path. Keywords: Exhibition; narrative; knowledge; agnosis. Photography and image editing: Maíra Acioli Data Sheet of the exhibition Experimental exercise of shared curation, project and expographic narrative, required as required as completion of Expography I and II (2018) courses of the Undergraduate Museology Course of UFPE, under the supervision of Professor Antonio Motta. Curation / Expographic Project / Setting Bárbara Gondim Bezerra SilvaFabrício Tavares de Arruda dos SantosFrancisca Juscizete Queiroz de LimaManoel Francisco da Silva NetoStella Victoria Arcelino Bastos Lavra Graphic designOlívia Morim LightingCamila Maria Silva de Moraes Santos AcknowledgmentRecension: Prof. Lepê Correia RealizationDepartment of Anthropology and Museology | DAMDepartment Head Ana Cláudia Rodrigues da SilvaUndergraduate Museology Course | Coordinator Alexandro Silva de JesusExpography I e II courses (2018)Professor Antonio Motta