Дисертації: "DDAE"

1

Vidal, Jean Baptiste. "Système d'aide à la décision pour une planification industrielle et commerciale adaptative." Electronic Thesis or Diss., Ecole nationale des Mines d'Albi-Carmaux, 2023. http://www.theses.fr/2023EMAC0016.

Повний текст джерела

Анотація:

L'approche de l'entreprise adaptative basée sur la demande (Demand Driven Adaptive Enterprise DDAE), proposée par le Demand Driven Institute (DDI), a suscité un intérêt croissant parmi les universitaires et les professionnels ces dernières années. La présente recherche se penche sur le volet stratégique de ce modèle, spécifiquement sur le processus de planification adaptative des ventes et des opérations (Adaptive Sales and Operations Planning AS&OP). L'AS&OP est supposé procurer aux gestionnaires une visibilité permettant de maîtriser la variabilité, d'améliorer le retour sur investissement et de s'adapter aux fluctuations fréquentes du marché. Bien que ces avantages aient été revendiqués précédemment par d'autres méthodologies, bon nombre d'entre elles n'ont pas réussi à concrétiser ces promesses. En conséquence, l'AS&OP a capté l'attention de nombreux professionnels. Cependant, peu de recherches formelles ont encore validé son efficacité, et aucun outillage opérationnel n'a été établi. Cette thèse de doctorat évalue la pertinence et la portée du processus AS&OP. L'objectif a été de cerner les limitations des méthodes actuelles dans la littérature, et de mettre en avant les perspectives à approfondir, telles que le risque, le flux, la finance, la capacité, le marché, etc., ainsi que les variables décisionnelles, les mécanismes de coordination, les structures organisationnelles, et les indicateurs de performance. En pratique, cette recherche présente le développement d'un système d'aide à la décision AS&OP-DSS (Decision Support System) pour une simulation dynamique, incluant méthodologie et algorithmie associées. En premier lieu, un modèle agrégé de l'approche DDMRP (Demand Driven Requirement Planning) est élaboré et intégré au sein de ce système. Par la suite, une stratégie pour inclure les incertitudes, risques, et opportunités est proposée, tout en évaluant chaque scénario via des indicateurs de performance ad hoc. Ce système propose de faciliter la génération et l'évaluation de scénarios en utilisant une simulation dynamique basée sur la planification scénarisée (What-if). Enfin, la proposition intègre une approche capacitaire de la planification afin de mieux supporter les décisions stratégiques à prendre. Les résultats de cette recherche sont corroborés à chaque étape par des études de terrain, impliquant des entreprises adoptant l'approche DDAE. Ainsi, l'étude est structurée autour de deux axes principaux : une dimension théorique et une dimension pratique
The Demand Driven Adaptive Enterprise (DDAE) approach, proposed by the Demand Driven Institute (DDI), has attracted growing interest among academics and professionals in recent years. The present research focuses on the strategic side of this model, specifically on the Adaptive Sales & Operations Planning (AS&OP) process. AS&OP is supposed to provide managers with visibility to control variability, improve ROI and adapt to frequent market fluctuations. Although these benefits have been claimed previously by other methodologies, many have failed to deliver on these promises. As a result, AS&OP has captured the attention of many professionals. However, little formal research has yet validated its effectiveness, and no operational tools have been established. This doctoral thesis evaluates the relevance and scope of the AS&OP process. The aim has been to identify the limitations of current methods in the literature, and to highlight perspectives to be explored further, such as risk, flow, finance, capacity, market, etc., as well as decision variables, coordination mechanisms, organizational structures, and performance indicators. In practice, this research presents the development of an AS&OP-DSS decision support system for dynamic simulation, including associated methodology and algorithmics. First, an aggregated model of the DDMRP (Demand Driven Requirement Planning) approach is developed and integrated within this system. Subsequently, a strategy for including uncertainties, risks and opportunities is proposed, while evaluating each scenario via ad hoc performance indicators. This system proposes to facilitate scenario generation and evaluation using dynamic simulation based on what-if planning. Finally, the proposal integrates a capability-based approach to planning in order to better support the strategic decisions to be taken. The results of this research are corroborated at each stage by field studies involving companies adopting the DDAE approach. Thus, the study is structured around two main axes: a theoretical dimension and a practical dimension

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Tran, Cam Thanh Lucy. "Molecular analysis of human DDAH genes." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408021.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Tommasi, Sara. "Design and synthesis of human dimethylarginine dimethylaminohydrolase (DDAH) inhibitors and development of a novel DDAH activity assay." Thesis, University of Aberdeen, 2015. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=227616.

Повний текст джерела

Анотація:

Nitric oxide (NO) is a key physiological messenger, but an excessive production of this molecule can be detrimental, leading to the onset or worsening of many pathological conditions. Dimethylarginine dimethylaminohydrolase (DDAH) is a key enzyme in the NO pathway, involved in the metabolism of asymmetric dimethylarginine (ADMA) and monomethyl arginine (L-NMMA), which are both endogenous inhibitors of NO synthesis. Two isoforms of DDAH have been identified in humans, namely DDAH-1 and DDAH-2. DDAH inhibition represents a promising strategy in the treatment of NO overproduction under pathological conditions without affecting the homeostatic role of this messenger. In this work I described the design and synthesis of 12 novel potential DDAH inhibitors together with the development of a new UPLC-MS based assay to measure the activity of HEK293T cell lysates overexpressing recombinant human DDAH-1 in metabolizing ADMA into dimethylamine and L-citrulline. The same assay was used to assess the potential of the novel compounds, as well as of the well-known DDAH inhibitor L-257, to inhibit DDAH-1 catalyzed L-citrulline formation from ADMA. Three of the novel molecules (compounds 10a, 14a and 14b) showed very interesting inhibitory activity: in particular, the methylacylsulfonamide analogue of L-257 (10a) resulted in 13-fold higher inhibition potency than L-257 itself (98% of inhibition at 1mM, IC50 = 3±3 μM and Ki = 1±0 μM). This molecule was chosen for molecular dynamics simulations to study the putative mechanism for 10a inhibition of DDAH-1 activity. Furthermore, DDAH-1 and DDAH-2 were engineered introducing a FLAG-tag at the C-terminal of the proteins to allow their purification from the lysate components by immunoprecipitation. Although the purification protocol requires some further improvement, the fusion proteins did not show to be functionally affected by the modification.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Xie, Chen. "DYNAMIC DECISION APPROXIMATE EMPIRICAL REWARD (DDAER) PROCESSES." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1398991609.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Silva, Tarciana Dias da. "DDAN: A distributed directory for ambient networks." Universidade Federal de Pernambuco, 2008. https://repositorio.ufpe.br/handle/123456789/2130.

Повний текст джерела

Анотація:

Made available in DSpace on 2014-06-12T15:54:45Z (GMT). No. of bitstreams: 2 arquivo2010_1.pdf: 5876165 bytes, checksum: 604cf585e6b37b930842351c554aa528 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2008
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Dias da Silva, Tarciana; Fawzi Hadj Sadok, Djamel. DDAN: A distributed directory for ambient networks. 2008. Dissertação (Mestrado). Programa de Pós-Graduação em Ciência da Computação, Universidade Federal de Pernambuco, Recife, 2008.

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Tornazakis, Ioannis. "Development of a Distributed Digital Array Radar (DDAR)." Thesis, Monterey, California. Naval Postgraduate School, 2008. http://hdl.handle.net/10945/4000.

Повний текст джерела

Анотація:

Distributed digital arrays have many potential applications in radar and communication systems. The objective of this thesis is to re-examine previous research on distributed digital array radar (DDAR) and evaluate several critical aspects of a proposed wireless architecture. Self-standing transmit/receive (T/R) modules are synchronized wirelessly. An important issue addressed in this thesis is whether a simple low-cost synchronization circuit would perform adequately. To this end two breadboard T/R modules were built to support test and evaluation. Both measurements and simulations were performed. Other issues addressed in the research include a comprehensive investigation of the demodulator performance, and the development of Controller and processing software in LabVIEW.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Djerf, Pontus R. Tornazakis Ioannis. "Development of a Distributed Digital Array Radar (DDAR)." Monterey, Calif. : Naval Postgraduate School, 2008. http://edocs.nps.edu/npspubs/scholarly/theses/2008/Sept/08Sep%5FDjerf.pdf.

Повний текст джерела

Анотація:

Thesis (M.S. in Electronic Warfare Systems Engineering)--Naval Postgraduate School, September 2008.
Thesis Advisor(s): Jenn, David. "September 2008." Description based on title screen as viewed on November 3, 2008. Includes bibliographical references (p. 153-155). Also available in print.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Pullamsetti, Soni. "Role of Dimethylarginine Dimethylaminohydrolases (DDAH) in pulmonary arterial hypertension." Giessen VVB Laufersweiler, 2006. http://geb.uni-giessen.de/geb/volltexte/2006/2892/index.html.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Woo, Chelsea So-Ming. "Efficacy of tebuconazole and ddac in shell-treated wood." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/30484.

Повний текст джерела

Анотація:

In 2008, the Canadian standard for decking installed above ground was revised, and the penetration requirement was eliminated. This decision was based on field test data and fundamental work on mobility of copper in the preservative formulations that dominated the market at the time. Recently the wood protection industry has shown interest in shifting towards carbon-based preservatives. Thus, it is important to test the efficacy of carbon-based preservative formulations as shell-treatments on Canadian wood species. In this study, samples of spruce heartwood were treated with a formulation containing either tebuconazole or didecyldimethylammonium carbonate (DDAC). The treated wood was exposed outdoors for one year and the leachate from these samples was collected. Tebuconazole and DDAC were detected in the leachate collected, and this indicates that these active ingredients were mobile in the wood after treatment. DDAC was detected in very low concentrations on wood surfaces that were untreated before exposure: 0.03 mg DDAC/g of wood and 0.02 mg of DDAC/g wood were measured for the high and low retentions respectively. The concentration of tebuconazole detected was not different from the control samples. This suggests that mobile DDAC may be able to re-deposit in the wood, but tebuconazole does not re-deposit once it is dislodged from the wood. Furthermore, results showed that spores of Gloeophyllum sepiarium and Oligoporus placentus were able to germinate on untreated check surfaces within 2 weeks on samples collected from exposed, treated wood. This indicates that the re-deposited carbon-based active ingredients were not able to protect the untreated check surfaces against germination of basidiospores of some common fungi isolated from above-ground decking in Canada.

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Tomlinson, James. "The role of DDAH and ADMA in kidney disease." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/24541.

Повний текст джерела

Анотація:

Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis and elevated plasma levels associate with poor cardiovascular and renal outcomes. The dimethylarginine dimethylaminohydrolase enzymes (DDAHs; 1 and 2) metabolise ADMA. A DDAH1 gene variant associates with higher kidney tissue mRNA expression, lower plasma ADMA but counter-intuitively, a steeper rate of eGFR decline. This indicates that renal DDAH1 activity may be deleterious and circulating ADMA does not necessarily reflect the NO-ADMA balance (or severity of disease) within kidney tissue. This study tests the hypothesis that reduced renal DDAH1 activity protects against the progression of kidney function decline, independent of circulating ADMA. Renal DDAH1 expression predominates within the proximal tubule. A novel proximal tubule-specific DDAH1 knock-out (PTD1KO) mouse was developed, which demonstrated tubule-specific dysregulation of ADMA and NO that was not evident systemically. Phenotyping studies in PTD1KO mice did not identify consistent alterations of urinary biochemistry at baseline or after salt loading, however, proteomic analysis revealed significant alterations of urinary peptides at baseline; including down-regulation of uromodulin and collagen. At 12 weeks following folate renal injury, the PTD1KO mouse exhibited less kidney function decline, collagen deposition and pro-fibrotic gene expression (Col12alpha, TGFbeta and ET-1) than controls. Furthermore, ADMA and DDAH1 inhibition reduced tubular sodium and fluid reabsorption in rat microperfusion studies, although studies in PTD1KO mice failed to reproduce this effect. Finally, in vitro studies using a PT cell line and primary PT culture indicated an inhibitory effect of ADMA upon PT cell proliferation. Consistent with recent human genetic studies, these data provide experimental evidence indicating a reduction of renal tubule DDAH1 activity can protect against progressive kidney fibrosis and function decline, independent of plasma ADMA. This work provides novel insights into the role of the NO-ADMA-DDAH axis within the kidney, particularly the tubule.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Janakiraman, Laxmipreethi. "Deep Directive Attention Network(DDAN) based Sign Language Translation." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/26581.

Повний текст джерела

Анотація:

Sign language is a visual language. It is an effective way of communication for hearing and speech impaired community. In general, all visual languages are multi-modal which utilizes hand gestures, facial expressions and other non-manual features to effectively consider the linguistics of the language while communicating with others. In the recent few years, due to the advancement of computer vision and the NLP field, Sign Language Recognition(SLR) and Sign Language Translation(SLT) topic has attracted many researchers to find an effective way to translate sign language videos to spoken language sentence. Over a decade many ideations have been published but most of them focused on SLR as a mere gesture recognition problem without considering the linguistic structure. In the literature review, we dive deep in to the understanding of various Senor and vision based approaches which were used in the earlier days followed by Deep learning techniques which are offering state-of-the-art results in the recent days. Applying a mid-level Sign Gloss Representation is a key component of performing a successful SLT. Hence, an effective joint learning of mid-level sign Gloss into the Text translation is crucial to improve the performance. In this dissertation, we propose Deep Directive Attention Network (DDAN)-based sign translation framework that allows aligning key-tokens in sign Gloss with key-words in Text. Directive attention transformer is successfully used in this approach to have better inter-intra modal relationship between Gloss sequences and Text sentences which aids in higher translation accuracy of Sign videos to Text sentences. The proposed DDAN contains the Self-Attention (SA) of each sign Gloss and Text, as well as the Gloss Directive-Attention (DA) of Text. Those two attention units, SA and DA, can be placed and integrated in three different proposed DDAN variants, including DA, SDA and SSDA. We evaluate the translation performance of our Sign2(Gloss+Text) and Gloss2Text approaches on the two challenging benchmark datasets PHOENIX-Weather 2014T and ASLG-PC12. The data statistics were analyzed as the first step. Then, three different model variants were evaluated on the above mentioned datasets. The model variant SSDA outperformed the baseline models in both datasets with higher translation accuracy of Sign videos to Text sentences as well as Gloss sequences to Text sentences . Furthermore, we evaluated on various numbers of encoder and decoder to see the optimal count of layers in which the model outperformed the baselines. The hyper-parameter testing result shows the robustness of the proposed framework. In addition to quantitative analysis results, we also provide the qualitative results of the evaluations which shows the generated text sentences has translation precision close to gold standard text along with evident improvement in the morpho-syntax. Based on all the evaluations and analysis results, we demonstrate that out DDAN-based SLT framework outperforms all the state-of-the-art SLT models and achieve better and higher translation accuracy score.

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Artman, Anna. "Avvattning av nanocellulosa i en DDA." Thesis, KTH, Skolan för kemivetenskap (CHE), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-170123.

Повний текст джерела

Анотація:

Genom laborativa försök skulle avvattning och retention av nanocellulosa i en DDA (Dynamic drainage analyzer) undersökas. Detta genom att tillsätta retentionskemikalier i varierande mängder för att se hur det påverkade avvattningen. Uppdragsgivaren var Innventia och laborationerna utfördes på SP:s laboratorium där DDA instrument fanns tillgängligt. DDA instrumentet liknar den maskin som används för papperstillverkning och därför anses det vara möjligt att kunna avvattna nanocellulosa på liknande instrument. Målet med examensarbetet var att få fram en nanocellulosafilm med goda barriärsegenskaper men också se hur tillsats av bärarfibrer påverkar filmens egenskaper. Målet var också att se om retention och avvattningstiden för nanocellulosa hänger ihop och om det går att få fram repeterbara resultat. Två olika viror undersöktes också i de laborativa experimenten i DDA, en som benämns som Albanyviran och en som benämns som Stratexviran. Albanyviran är tätare än Stratexviran och effekten av viratäthet på retention och avvattningstid undersöktes. Nanocellulosa eller Mikrofibrillär cellulosa (MFC) är ett nytt och förnybart material som utvinns ur träfibrer och karakteriseras av sitt geléaktiga utseende[4]. Nanocellulosan lämpar sig utmärkt för en mängd olika produkter t.ex. som barriär, enskilt i form av filmer eller i blandade produkter. Vid tillverkning av nanocellulosa används en homogenisator som sönderdelar cellulosafibrer till fibriller och fibrillaggregat. Detta var tidigare ett problem då fibrerna satte igen homogenisatorn och tillverkningen var mycket energikrävande[4]. När det gäller filmtillverkning av nanocellulosa kvarstår problemet när det kommer till avvattningen. Nanocellulosan späddes till önskad koncentration och innan de laborativa försöken kördes den genom en homogenisator för att dispergera fibrillerna i vätskan efter spädningen. Bärarfibrerna slogs upp i en uppslagare med två liter kranvatten för att sedan tillsättas till den homogeniserade nanocellulosan. Under försöket i DDA varierades mängden och andelen MFC (mikrofibrillär cellulosa) och bärarfibrer (Modorefmassa). Till MFC och bärarfibersuspensionen i DDA:n tillsattes sedan två retentionskemikalier vid varje försök, C-PAM PL-1520 och EKA NP-780 i varierande mängder. Efter avvattningen i DDA:n pressades filmen vid olika tryck och tider för att därefter mäta filmernas ytvikt och syrgasbarriär. Den film som ansågs mest lämplig gällande avvattning i DDA under försöken var vid 0,2 % med 90 % MFC och 10 % bärarfibrer. Filmen gav den högsta retentionen, en god syrgasbarriär och var lätt att hantera. Det som kan ses från resultaten av syrgasbarriären är att vid 0,2 % med 90 % MFC och 10 % bärarfibrer erhölls det lägsta OTR (oxygen transmission rate) -värdet på 0,53 vilket visar på en bra syrgasbarriär. Retentionen för det försöket var det högsta på 87,1 % medan avvattningstiden låg på närmare 250 sekunder. Avvattningstiderna var höga, dock så var det vid denna totalkoncentration ibland svårt att se när avvattningen avslutades då tiden klockades manuellt. Det som kan ses utifrån resultaten är att bärarfibrerna inte påverkar filmerna negativt utan kan gynna både så retentionen och syrgasbarriären blir bättre, dock fås en högre ytvikt och avvattningstiderna blir längre.
Through laboratory experiments, dewatering and retention of nanocellulose in a DDA (Dynamic Drainage Analyzer) were analysed. By adding retention chemicals in varied amounts, the effects on the dewatering was shown. The Job initiator was Innventia and the laboratory work were made at SP's laboratory where the DDA instrument was available. The DDA instrument is similar to the machine that is used for paper manufacturing in a large scale and therefore, it could be possible to dewater nanocellulose on a similar instrument. The goal of the thesis was to develop a nanocellulose film with good barrier properties but also to see how adding carrier fibers effect the properties of the film. The goal was also to see if the retention and dewatering time of nanocellulose are connected and whether it is possible to obtain repeatable results. Two different wires were also examined in the laboratory experiments in a DDA, the Albanywire and the Stratexwire. The Albany wire was denser than the Stratex wire and the effect that the density caused on retention and dewatering time was examined. Nanocellulose or Microfibrillated cellulose (MFC) is a new and renewable material that is made from wood fibers and is characterized by its gelatinous appearance. [4] Nanocellulose is suited for a variety of products, such as barriers, alone in the form of films or mixed in products. In the manufacture of nanocellulose a homogenizer is used which decomposes cellulose fibers to fibrils fibril aggregate. This was previously a problem while the fibers clogged the homogenizer and the production had a high energy consumption. [4] When it comes to making a nanocellulose film the problems with dewatering remains. The nanocellulose was diluted to the desired concentration and before the laboratory experiments it was run through a homogenizer, to disperse the fibrils in the liquid after the dilution. The carrier fibers was prepared in a blender with two liters of tap water before it was added to the homogenized nanocellulose. During the experiment in the DDA the amount and proportion of the MFC (microfibrillar cellulose) and carrier fibers (Modorefmassa) was varied. To the MFC and carrier fiber suspension in the DDA two retention chemicals were added in each experiment, C- PAM PL -1520 and EKA NP- 780 in varying amounts. After the dewatering of nanocellulose in the DDA the films were pressed at different pressures and times, thereafter the oxygen permeability was analyzed. The film that was considered the most suitable referring to dewatering in the DDA during the attempts was at 0.2 % with 90 % MFC and 10 % carrier fibers. The film gave the highest retention, a good oxygen barrier and was easy to handle. What can be seen from the results of the oxygen barrier measurement is that at 0.2 % with 90 % MFC and 10% carrier fibers obtained the lowest value OTR (oxygen transmission rate), which indicates on a good oxygen barrier. Retention at this concentration was the highest at 87.1 %, while the drainage time was nearly 250 seconds. The dewatering time was high, however during this concentration it’s sometimes difficult to see when the dewatering ended while the time was clocked manually. Conclusions from the results are that the carrier fibers doesn’t have a negatively effect on the films, rather they can benefit both the retention and oxygen barrier, however a higher paper weight was obtained and the dewatering time became longer.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Shirley, Matt, and n/a. "Characterisation of an 84 kb linear plasmid that encodes DDE cometabolism in Terrabacter sp. strain DDE-1." University of Otago. Department of Microbiology & Immunology, 2006. http://adt.otago.ac.nz./public/adt-NZDU20060804.094902.

Повний текст джерела

Анотація:

DDT, an extremely widely used organochlorine pesticide, was banned in most developed countries more than 30 years ago. However, DDT residues, including 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), still persist in the environment and have been identified as priority pollutants due to their toxicity and their ability to bioaccumulate and biomagnify in the food chain. In particular, DDE was long believed to be "enon-biodegradable"e, however some microorganisms have now been isolated that are able to metabolise DDE in pure culture. Terrabacter sp. strain DDE-1 was enriched from a DDT-contaminated agricultural soil from the Canterbury plains and is able to metabolise DDE to 4-chlorobenzoic acid when induced with biphenyl. The primary objective of this study was to identify the gene(s) responsible for Terrabacter sp. strain DDE-1�s ability to metabolise DDE and, in particular, to investigate the hypothesis that DDE-1 degrades DDE cometabolically via a biphenyl degradation pathway. Catabolism of biphenyl by strain DDE-1 was demonstrated, and a biphenyl degradation (bph) gene cluster containing bphDA1A2A3A4BCST genes was identified. The bphDA1A2A3A4BC genes are predicted to encode a biphenyl degradation upper pathway for the degradation of biphenyl to benzoate and cis-2-hydroxypenta-2,4-dienoate and the bphST genes are predicted to encode a two-component signal transduction system involved in regulation of biphenyl catabolism. The bph gene cluster was found to be located on a linear plasmid, designated pBPH1. A plasmid-cured strain (MJ-2) was unable to catabolise both biphenyl and DDE, supporting the hypothesis that strain DDE-1 degrades DDE cometabolically via the biphenyl degradation pathway. Furthermore, preliminary evidence from DDE overlayer agar plate assays suggested that Pseudomonas aeruginosa carrying the strain DDE-1 bphA1A2A3A4BC genes is able to catabolise DDE when grown in the presence of biphenyl. A second objective of this study was to characterise pBPH1. The complete 84,054-bp sequence of the plasmid was determined. Annotation of the DNA sequence data revealed seventy-six ORFs predicted to encode proteins, four pseudogenes, and ten gene fragments. Putative functions were assigned to forty-two of the ORF and pseudogenes. Besides biphenyl catabolism, the major functional classes of the predicted proteins were transposition, regulation, heavy metal transport/resistance, and plasmid maintenance and replication. It was shown that pBPH1 has the terminal structural features of an actinomycete invertron, including terminal proteins and terminal inverted repeats (TIRs). This is the first report detailing the nucleotide sequence and characterisation of a (linear) plasmid from the genus Terrabacter.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Liu, Ruiying. "The influence of didecyldimethylammonium chloride (DDAC) treatment on wood weathering." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25097.pdf.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Grochal, Peter. "Návrh DDA zesilovače pro zpracování biologických signálů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442591.

Повний текст джерела

Анотація:

The work deals with the analog design of low-voltage and low-power differential difference amplifier DDA with adaptive differential input stage, second stage class AB, improved by a self – cascode to achieve higher gain and slew rate. Conventional and unconventional techniques, and methods for low-voltage and low-power design are presented. The finished design of the differential difference amplifier DDA with the analyzed results is presented. Design of a Butterwortho low-pass filter of the sixth order based on DDA with Sallen Key topology and design of a multifunctional ARC filter based on DDA.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Mangoldt, Charlotte von. "Student environmentalism in Beijing, China." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:ef524063-dda5-4cda-a73a-f0d56b95f527.

Повний текст джерела

Анотація:

This dissertation explores student environmentalism in Beijing, China. It traces students' political norms and values, explains their activism and experience of pollution, and investigates the role of environmental non-governmental organisation (ENGOs) in forming youth environmentalism. To serve these objectives, the work takes forward theories on youth activism and agency and recent debates on environmental health, environmentalism and ENGOs. This study was designed as a qualitative research project based primarily on interviews and complemented by ethnographic methods, content analysis, pictorial evidence and survey results provided by the Jane Goodall Institute China. Research findings and methodology are presented in four papers and a framing document. My work challenges labels of agency and activism as either protest and resistance or 'quiescence' and questions the influence of globalisation on activists' norms and values. I put forward 'fragmented activism' as a new concept to capture the nature of youth environmental activism in Beijing. I contribute to environmental health literature by tracing how young people develop discursive mechanisms to mitigate the fear of air pollution and argue that their response offers invaluable insights into the interplay between space and the body in polluted environments. This thesis further shows that the repertoire of student environmental associations in Beijing represents a type of 'place based environmentalism' (Smith, 2001) but argues that, whilst this may be a contradictory response to contemporary environmental issues, it is not usefully assessed against abstract and normative notions of what environmentalism should be. I also challenge scholarly assessments of ENGO action. By exploring ENGO strategies in China that rely on extant societal and governmental narratives about good citizenship and moral values - instead of radical alternatives to mainstream development models or political processes - I argue for new research paradigms guiding the study of environmental movements.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Hicks, Diana. "English language teaching teacher's guides : a critical discourse analysis of three texts." Thesis, University of Bristol, 2000. http://hdl.handle.net/1983/a13246cc-dda1-4a94-b061-7c3a415ee82e.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Weberruß, Lukas. "Erweiterung von MoleOffice um gemeinsame Dokumentenbearbeitung." [S.l.] : Universität Stuttgart , Fakultät Informatik , Institut für Parallele und Verteilte Höchstleistungsrechner, Abteilung Verteilte Systeme, 2000. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8619082.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Carvalho, Ana Luísa Esteves Leal Rodrigues de. "Adopção dde uma solução ECM : um estudo de caso." Master's thesis, Instituto Superior de Economia e Gestão, 2012. http://hdl.handle.net/10400.5/11007.

Повний текст джерела

Анотація:

Mestrado em Gestão de Sistemas de Informação
As organizações, cada vez mais, vêm-se confrontadas com a necessidade de gerir os conteúdos não estruturados de forma a se tornarem mais competitivas. Nesse sentido, o conceito de Enterprise Content Management (ECM) tem vindo a ter grande destaque nos últimos anos e as organizações começaram a implementar soluções para gerir os seus conteúdos. No entanto, a nível académico e científico, não tem tido o mesmo relevo. Neste contexto, a questão de investigação colocada foi: ?Como adotar um Enterprise Content Management?? com o objetivo de propor uma metodologia para adotar uma solução de Enterprise Content Management. Para a prossecução deste objetivo foi proposto um conjunto de passos ou etapas para a adoção do ECM e realizado um estudo de caso num organismo da administração pública. O principal contributo do presente trabalho é a proposta de uma metodologia para a adoção de uma solução de ECM. Pelo facto de se tratar de um tema recente e pela escassa literatura, este Trabalho de Fim de Mestrado pode contribuir para uma melhor compreensão do conceito de ECM e constituir um avanço no que se refere à sua adoção.
The organizations, more and more, have to deal with the need to manage unstructured data in order to become more competitive. Accordingly that, Enterprise Content Management (ECM) is a subject that has been given a great importance since recent years and the organizations have begun implementing these solutions to manage the unstructured data. Although at an academic and scientific level, it hasn't had the same relief. In this context, the research question was: "How to adopt an Enterprise Content Management?" in order to propose a methodology for adopting the Enterprise Content Management. To achieve the proposed goal was proposed a set of steps or stages for the adoption of ECM and also was conducted a case study in public administration. The main contribution of this work is to propose a methodology for adopting an ECM solution. Because it is a recent issue and there are few academic studies, this work can contribute to a better understanding of the concept of ECM and also constitute an advance in relation to deployment of the ECM.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Pullamsetti, Soni [Verfasser]. "Role of dimethylarginine dimethylaminohydrolases (DDAH) in pulmonary arterial hypertension / vorgelegt von Soni Pullamsetti." Giessen : VVB Laufersweiler, 2006. http://d-nb.info/98866240X/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Kleyer, Niklas Carl Simom. "TRAFFIC SIMULATIONS THROUGH ODE-, DDE SYSTEM MODELING AND NUMERICAL COMPUTATIONS." Thesis, KTH, Matematik (Inst.), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-148377.

Повний текст джерела

Анотація:

This report is written as a project to conclude the three year bachelor part of a five year degree in engineering and will as such target students finishing their three year bachelor degrees. The main topic of this project is traffic simulation through numerical analysis, with the accompanying subtopics time integration, eigenvalue analysis and computation complexity. The tool used through the project is Matlab computational software. This project features two general traffic models where the first is based on a system of ordinary differential equations (ODE’s) and the second one is based on a system of delay differential equations (DDE’s). The project will highlight the implications of driver considerations in terms of stability, stability related to system size, stability related to reaction times and the relation between the large system stability and increasing the (h; k) values of the model. (h; k) values of the model
The ODE- and DDE models are based on the same model with the only exception that the DDE model features reaction times. They are defined by consideration forces and sub-consideration forces. The values (h; k) determines the number of cars that each driver considers and therefore adds to the system as additional terms that are of the same form as the consideration forces, hence the sub prefix. The basic case where there are no sub-considerations involved is called the base case of the system and equals to (h; k) = (1; 1). The (h; k) of the system is determining the matrix B in equation (11) by the number of sub diagonals h and super diagonals k that are filled by weights of the forces. The time integrations can result in three base cases, unstable, stable oscillating and exponentially stable. These cases refer to the behavior of all system velocities. The unstable case can for limited time frames predict collisions between cars but otherwise diverge and cannot generally be used. Oscillating stable systems reach a constant velocity after a settling time and fits well into a realistic scenario. The exponential case reaches a constant velocity the fastest and is therefore the sought after solution. Both models are similar in this regard apart from the fact that the DDE model generally have a lot more system energy. Figures 1 and 4 are empirical proof that the models works as defined and can predict some traffic behavior. An interesting observation during testing was that the ODE exponential case would always remain exponential no matter the multiplication (; ; ) = C(; ; ), the only difference would be the system energy since larger acting forces are coupled with larger energies. The DDE model however is dependent on the system energy for stability since the delay sets a system energy limit for stability since too large forces coupled with delay will not achieve the optimum distance d. The system stability analysis can be reduced in both models to analyzing the homogeneous and particular parts separate. The expansions confirms in both cases what the time integrations shows and can give an idea of how the stability changes with one parameter changing. However, this is where the DDE model behaves completely different from the ODE model. For the ODE case it is possible to plot a complete eigenvalue chart whereas the DDE case has an infinite number of eigenvalues and is therefore impossible to completely chart. A conclusion that is in common between the models is that Fd(t) inherently is dominant and should as such be at lower priority compared to the other consideration forces in order to help system stability. Comparisons to the spring equation revealed that systems that prioritize Fd(t) too high converges to a system of particles in a chain connected by springs with no friction giving the observed behavior. Prioritizing F fr(t) help stability in both cases with the exception that DDE case will be stable for a sub interval (since the top limit comes from the system total energy) within the expansion whereas the ODE case remains stable through the whole intervall. The problems that come with larger systems are stability- and computation complexity related. All through the project has the models focused on the base case with no sub-considerations. The thesis is that adding sub-considerations will again stabilize an unstable system with the addition that each consecutive weight should deflate its value exponentially. The results proves that an unstable system can be stabilized by simply increasing the (h; k) of the system. This can have applications when the optimal weights are not enough to stabilize a large system. When computing the eigenvalues for large systems it puts strain on the algorithm ’eig’. According to [1] is the computation time complexity proportional to n3. What the resulting fit shows is that the relation is more quadratic than cubic and the reason is described to be the appearance of the system matrix for the base case. The matrix structure is similar to the one of the upper Hessenbergs which as a result saves time when transforming the input matrix which is the reason why the complexity is weakly cubic.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Clatworthy, David Elland. "A new method of meshing in discontinuous deformation analysis (DDA)." Master's thesis, University of Cape Town, 1997. http://hdl.handle.net/11427/16089.

Повний текст джерела

Анотація:

Includes bibliography.
Discontinuous Deformation Analysis (DDA) is a discrete element method developed by Shi [1988] specifically for modelling blocky rock masses. The DDA method is based on the assumption that deformation and failure of such rock masses is primarily due to differential movements of rock blocks, rather than strain and fracture of intact rock material. Strains and stresses are assumed to be constant over the area of each rock block. Contact between blocks is modelled using penalty functions, with Coulomb's friction law controlling sliding along block boundaries. Tests show that while DDA is not well suited to dynamic simulations where the velocities of blocks become large, it can model rock masses to a reasonable degree of accuracy in static analyses. There are various analysis control parameters which have a marked effect on the solution, however, and the user should take care in choosing suitable values for these parameters. A method is proposed here, in which certain blocks can be sub-divided into Finite Element meshes in order to obtain a more accurate description of their deformation. The method takes advantage of the fact that both DDA and the Finite Element Method (FEM) use the principle of stationary potential energy to obtain the solution equations for block equilibrium. Both DDA blocks and FEM elements can therefore initially be treated as DDA blocks, using the standard DDA formulation, and then the solution equations for the FEM elements are converted into Finite Element format by a simple transformation procedure before solution. First and second order DDA blocks are considered in this report, along with their equivalents in FEM, the C0-linear and C0-quadratic triangular elements. The C0-linear elements are found to be too stiff in modelling bending deformation, due to the assumption of constant strain throughout the element. The C0-quadratic elements are able to accurately model bending, however. It is shown through tests that the performance of these FEM elements, formulated within the DDA method, is identical to that obtained using the corresponding elements in conventional Finite Element programs. The sub-meshing method therefore allows mixed-formulation analyses, with DDA blocks and FEM meshes interacting within a single system, while remaining efficient, and reasonably simple to incorporate into existing DDA program codes. It would also be possible to model material non-linearity and fracture using this method.

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Wu, Bo. "Einbau weiterer Desktop Publishing Systeme in DDE (Distributed Document Environment)." [S.l.] : Universität Stuttgart , Fakultät Informatik, 2000. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8536752.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Reigner, Hélène. "Les DDE et le politique : quelle co-administration des territoires ? /." Paris ; Budapest ; Torino : l'Harmattan, 2002. http://catalogue.bnf.fr/ark:/12148/cb388931806.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Maruyama, Fábio Massatoshi. "Análise estrutural dinâmica de um vaso de pressão (reator PWR) utilizando o método DDAM." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/3/3135/tde-26072013-144247/.

Повний текст джерела

Анотація:

O objetivo deste trabalho é verificar a aplicação do Dynamic Design Analysis Method DDAM na análise de estruturas submetidas a carregamentos de choque. Esse método foi desenvolvido pela Marinha Norte Americana e se propõe a estimar esforços inercias provenientes das respostas de equipamentos de bordo, sejam de navios ou submarinos, a cargas impulsivas, mais precisamente, ondas de choque submarinas. Para a compreensão plena dessa metodologia, foi necessário um estudo sobre vibrações e espectros de resposta, tópicos teóricos que fundamentam esse procedimento. Devido à escassez de informações divulgadas por se tratar de conteúdo militar, foi realizado um estudo de caso para uma estrutura simples, uma viga engastada e seus resultados foram comparados com um prévio trabalho publicado. Com isso, foi possível a aplicação do DDAM em uma estrutura mais complexa como o vaso do reator nuclear do tipo PWR de um submarino, ainda que em um modelo simplificado desse equipamento. No entanto, os resultados mostraram a validade do uso do DDAM na análise estrutural dinâmica de equipamentos de uma maneira qualitativa.
The main purpose of this dissertation is to verify the use of the Dynamic Design Analysis Method DDAM in the study of structures subjected to shock loadings. This method was developed by the U.S. Navy and is intended to estimate inertial forces resultant from the response of shipboard equipment, from surface ships or submarines, due to impulsive loads or, more precisely, underwater pressure shock waves. To fully comprehend this method, it was necessary to acknowledge fundamentals of dynamics of structures like free vibrations, response spectrum analysis correlated topics. And due to lack of public information since it is a military technology, a case study of a cantilever beam was done with its results compared to a previous published paper. Then, the DDAM was applied to a more complex structure a PWR nuclear reactor vase simplified model, in order to show the effectiveness of the method in dynamic structural analysis.

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Stefansson, Kolbeinn. "Economic inequality and social class." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:33ce091f-dda6-42cc-a824-c6407e5cd265.

Повний текст джерела

Анотація:

This thesis is about social class and economic inequality, using the Goldthorpe class schema. It tests theories claiming that social class is increasingly irrelevant to inequality and people's life-chances with data on incomes and material living standards from the British Household Panel Survey. It covers the period over which the survey ran, i.e. 1991-2008. During this time many prominent social theories dismissed class analyses while others sought to retain the class concept but dismissed its economic foundations, seeking to ground it in culture instead. Economic inequality has not figured highly on the agenda of class analysts, at least not those working with the Goldthorpe class schema. There is a substantial body of work on mobility, voting behaviour, income poverty and material deprivation, but inequality in a broader sense has for the most part been neglected. This thesis is a step towards rectifying this situation. Thus it provides new information about within-career social mobility as well as income inequality within and between classes, on whether income mobility reduces class inequalities over time, and cast light on class inequalities in material living standards. The findings suggest that class is far from irrelevant to economic inequality. Class differences in incomes are persistent, between class inequalities contribute more to inequality overall than within-class inequalities, and while income mobility does reduce class inequalities over time it is not to the extent that supports the hypothesis that class is irrelevant to people's economic fortunes.

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Ribeiro, Fernandes Hugo José. "Elucidating the role of GBA in the pathology of Parkinson's disease using patient derived dopaminergic neurons differentiated from induced pluripotent stem cells." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:7027574c-dda4-4752-9010-4c573bd0b2aa.

Повний текст джерела

Анотація:

Heterozygous mutations in the glucocerebrosidase (GBA) gene represent the most common risk factor for Parkinson’s disease (PD), a disease in which midbrain dopaminergic neurons are preferentially vulnerable. However, the mechanisms underlying this association are still unknown, mostly due to the lack of an appropriate model of study. In this thesis, we aimed at elucidating the role of heterozygous GBA mutations in PD using a specific human induced pluripotent stem cell (hiPSC)-based model of disease. First we developed a protocol for the efficient differentiation of hiPSCs into dopaminergic cultures, and extensively characterized the derived dopaminergic neurons which expressed multiple midbrain relevant markers and produced dopamine. Next we screened a clinical cohort of PD patients to identify carriers of GBA mutations of interest. Using for the first time hiPSCs generated from PD patients heterozygous for a GBA mutation (together with idiopathic cases and control individuals) we were able to efficiently derive dopaminergic cultures and identify relevant disease mechanisms. Upon differentiation into dopaminergic neuronal cultures, we observed retention of mutant glucocerebrosidase (GCase) protein in the endoplasmic reticulum (ER) with no change in protein levels, leading to upregulation of ER stress machinery and resulting in increased autophagic demand. At the lysosomal level, we found a reduction of GCase activity in dopaminergic neuronal cultures, and the enlargement of the lysosomal compartment in identified dopaminergic neurons suggesting a decreased capacity for protein clearance. Together, these perturbations of cellular homeostasis resulted in increased release of α-synuclein and could likely represent critical early cellular phenotypes of Parkinson's disease and explain the high risk of heterozygous GBA mutations for PD.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

McCann, Lisa. "Transition or transfer? : an experiential perspective on moving from paediatric to adult cancer services." Thesis, University of Dundee, 2012. https://discovery.dundee.ac.uk/en/studentTheses/80ae2c0e-ddce-47ae-a33f-6c680a35364e.

Повний текст джерела

Анотація:

This thesis explores the experiences of young people who are survivors of childhood cancer, their parents, health care professionals (HCPs), and case note documentation, of the process of transition from paediatric to adult cancer services. A qualitative, collective case study approach (Stake, 1995), informed by a constructivist-interpretive position, allowed exploration of the multiple realities prioritised in this study. Whilst there is a significant body of literature relating to transition for conditions such as rheumatology and cystic fibrosis, there is little research undertaken into transition in a cancer context, specifically so from an experiences perspective. This study aimed to re-address this issue. The experiences of twelve young people were explored in this study. This resulted in the participation of twelve cases, meaning a total of 35 individual, semi-structured interviews were conducted with young people, parents and HCPs. Young people’s case notes (22 sets) were also reviewed. Data were analysed using matrix-based approaches advocated by Miles and Huberman (1994), at both the within- and between-case level. This generated a multi-dimensional and multiple perspective understanding of the experience of the process of transition. The results of this study clearly identified a central orienting theme: The experience of readiness in the context of transition. Three main themes and six sub-themes were also identified within the data, supporting the explanatory power of the orienting theme. The main themes identified were: The experience of childhood cancer; Planning and preparation: Transition or transfer?; and A process of change. The findings demonstrate that understanding the multi-faceted components of readiness is crucial in understanding people’s experiences of readiness. Readiness should embody people’s illness experiences, the numerous and associated losses intertwined with a move from paediatric to adult care and the simultaneous developmental changes occurring in people’s lives. Only by doing so shall a meaningful understanding of the experience of the process of transition for survivors of childhood cancer, their parents and health care professionals be developed. The thesis concludes by making recommendations for future research and clinical practice.

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Belekdanian, Arto Onnig Arto Onnig. "The coronation ceremony during the eighteenth dynasty of Egypt : an analysis of three "coronation" inscriptions." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:4b606eb6-dd7e-4a7e-adf8-2234e11b01ef.

Повний текст джерела

Анотація:

This thesis provides a detailed interpretation of three key texts described in Egyptological research as "coronation inscriptions:" the Historical Inscription of Hatshepsut, Thutmose III's Texte de la jeunesse, and Horemheb's Turin inscription. Similarities and differences between these texts, as well as other sources, both textual and pictorial, are discussed. A clear terminology is laid out, distinguishing between accession (the royal heir becoming king at the death of their predecessor), crowning (the action of placing the crowns on the new king's head), and coronation ceremony (following the accession by some time on which occasion the new ruler would have been bestowed with the crowns and regalia of his office, perhaps for the first time). The main aim of this thesis is to determine whether it would be accurate to label the discussed texts as coronation inscriptions and, if not, how they can best be described. It is determined that the evidence supports the earlier conclusion reached by Redford, that it would be incorrect to speak of a âcoronation ceremonyâ in the dynastic period, for new kings would have been crowned at their accessions in a palace setting, soon after the death of their predecessors, this followed some time later by a public âappearance ceremonyâ in a temple festival setting. While it is determined that Thutmose III's inscription describes the time when kingship was predicted to him, it is concluded that the Hatshepsut and Horemheb texts narrate exceptional events on which occasion their accessions in a palace and public "appearance ceremonies" intersected.

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Steinhauser, Markus. "Family influences on strategic decision-making processes in family firms." Thesis, University of Brighton, 2014. https://research.brighton.ac.uk/en/studentTheses/11cd4b21-ddce-441d-8afb-63b09691e7b7.

Повний текст джерела

Анотація:

Family firms can be characterised by a unique business setting, where family and work conditions are closely intertwined. This particular business setting promotes distinctive strategic behaviour. Yet, scholarly understanding of strategic decision processes in family firms remains limited, particularly from a perspective that pays attention to the role of micro-processes in the overall decision-making process. This study addresses this gap from a strategy-as-practice perspective and by posing the research question: ‘how does the family context influence the strategic decision-making process in the family firm?’ The author uses a critical realist lens and case study method to examine multiple strategic decisions across 7 family firms.

Стилі APA, Harvard, Vancouver, ISO та ін.

31

McDonald, Valerie Alexandra. "Evaluating Immunotoxicity of Quaternary Ammonium Compounds." Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/79723.

Повний текст джерела

Анотація:

Alkyl dimethyl benzyl ammonium chloride (ADBAC) and didecyl dimethyl ammonium chloride (DDAC) are common quaternary ammonium compounds used as disinfectants in households, medical, and restaurant settings. They cause occupational skin and respiratory hazards in humans, and developmental and reproductive toxicity in mice. They also cause increased secretions of proinflammatory cytokines in cell lines and vaginal inflammation in porcine models; but have not been evaluated for developmental immunotoxicity. We assessed immunotoxicity in-vitro with J774A.1 murine macrophage cell line by analyzing cytokine production and phagocytosis; and evaluated developmental immunotoxicity in CD-1 mice by analyzing antibody production. Additionally, because of the associations between gut microbiome dysbiosis and immune disease, we monitored changes in the microbiome as a result of ADBAC+DDAC exposure. Production of cytokines TNF-alpha and IL-6 increased at low ADBAC+DDAC concentrations, and IL-10 decreased in the murine macrophages with ADBAC+DDAC exposure. The phagocytic function of macrophages was also severely decreased. ADBAC+DDAC altered the mouse microbiome by decreasing the relative abundance of Bacteroides and increases in Clostridia in F0 and F1 generations. IgG primary and secondary responses were altered in F1 male mice; and IgA and IgM production were decreased in secondary response in F2 male mice. Since ADBAC+DDAC show signs of immunotoxicity in mice, further studies are needed to reassess risk for human exposure as ADBAC+DDAC may be contributing to immune disease.
Master of Science

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Schurr, Peter W. "Erweiterung von DDE (Distributed Document Environment) um Konsistenzbehandlung in mobiler Umgebung." [S.l.] : Universität Stuttgart , Fakultät Informatik, 1999. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8385916.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Kupstaitytė, Brigita. "Saugumo užtikrinimas ekonominės diplomatijos priemonėmis: Kinijos, Indijos ir Pietų Korėjos lyginamoji analizė." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140620_105239-13767.

Повний текст джерела

Анотація:

Azijos žemyne formuojasi pasaulio geopolitinis-ekonominis centras. Vienos svarbiausių valstybių šiame procese yra Kinijos Liaudies Respublika (toliau Kinija), Indija ir Pietų Korėja. Geopolitinio-ekonominio centro formavimosi laikotarpiu komplikuotas ir probleminis tampa valstybių ekonominio saugumo užtikrinimas. Iškyla klausimas kokias priemones ir kuo remiantis pasitelkia šios valstybės savo ekonominio saugumo stiprinimui. Šio tyrimo objektas yra Kinijos, Indijos ir Pietų Korėjos ekonominė diplomatija. Darbo tikslas: pritaikyti ir kritiškai įvertinti Herscher-Ohlin-Vanek (HOV) modelį įvykdant Kinijos, Indijos ir Pietų Korėjos ekonominės diplomatijos lyginamąją analizę po įstojimo į Pasaulio Prekybos Organizaciją. Tikslui pasiekti iškeliami šie uždaviniai: 1) ištirti saugumo kaitą ir ekonominės diplomatijos sampratą; 2) įvertinti HOV modelį bei jo metodologinį pritaikymą Kinijos, Indijos ir Pietų Korėjos ekonominės diplomatijos analizei; 3) išanalizuoti esminius Kinijos, Indijos ir Pietų Korėjos ekonominės diplomatijos bruožus; 4) ištirti Kinijos, Indijos ir Pietų Korėjos ekonominės diplomatijos įrankių panaudojimą ekonominiam saugumui užtikrinti; 5) empiriškai palyginus Kinijos, Indijos ir Pietų Korėjos ekonominės diplomatijos įrankius kritiškai įvertinti HOV modelį. Tyrimui naudojami metodai: aprašomasis metodas, kokybinių ir kiekybinių metodų sintezė, ekonominės diplomatijos HOV modelis bei lyginamasis metodas. Atlikus tyrimą gauti rezultatai, jog HOV modelis daugeliu... [toliau žr. visą tekstą]
In Asia develops new world geopolitical-ecanomic centre. Of most important countries in this process are China, India and South Korea. Complicated period of geopolitical-economic centre formation rises vulnerabilies and challenges for the assurance of these countries economic security. Rises questions that means and why use these countries for their economic security strengthening. The object of this research is the economic diplomacy of China, India and South Korea. The goal is: to use and critically evaluate Herscher-Ohlin-Vanek model after accomplished comparative analysis of China, India and South Korea economic diplomacy after their join to the World Trade Organization. The tasks are: 1) to survey the fluctuation of security and conception of economic diplomacy; 2) to evaluate HOV model and its methodological application to the analysis of China, India and South Korea economic diplomacy; 3) to analyse essential features of China, India and South Korea economic diplomacy; 4) to examine China, India and South Korea economic diplomacy tools appliance for the assurance of economic security; 5) after empirical comparision of China, India and South Korea economic diplomacy tools to critically evaluate HOV model. For the research are used: descriptive method, the synthesis of qualitive and quantitive methods, economic diplomacy HOV model and comparative analysis. After research obtained results show that HOV model in most of cases can be used to explain countries‘ economic... [to full text]

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Zhang, Xi. "Erweiterung von DDE (Distributed Document Environment) um Workflow Management in mobiler Umgebung." [S.l. : s.n.], 2001. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9231990.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Khanom, N. "Evaluation of novel arginine based inhibitors of DDAH and investigations into radical hydroacylation of vinyl sulfonates." Thesis, University College London (University of London), 2010. http://discovery.ucl.ac.uk/192842/.

Повний текст джерела

Анотація:

The thesis is in two main sections. In the first section, studies on methylarginine processing enzymes are presented. Dimethyalrginine dimethylaminohydrolase (DDAH) is a class of enzymes involved in the metabolism of methylarginines ADMA and L-NMMA, which indirectly regulate physiological nitric oxide levels. It is desirable to inhibit excess NO in pathological situations, and the arginine mimetic L-257 is a DDAH inhibitor which reduces levels of NO. Synthesis of ester analogues of L-257 proved to be troublesome with a low yielding key guanidine forming reaction. However, amide analogues were readily synthesised, and testing for DDAH inhibition showed the dimethylamide analogue possessed similar activity to L-257. Further design and synthesis of a 7-membered cyclic analogue, based on the crystal structure of huDDAH1 with L-257, provided a novel analogue with no significant inhibition for rat kidney DDAH. Purified and isolated huDDAH2 protein showed activity after incubation with substrate L-NMMA. In the second part studies on aldehyde auto-oxidation are presented. Aldehydes autoxidise to their acids, via an acyl radical, which can undergo addition reactions with electron-deficient acceptors in a radical hydroacylation reaction. An α- iodo and α-chloro hexanal failed to autoxidise, however 7-hydroxycitronellal readily autoxidised and added to pentafluorophenyl(PFP)-vinyl sulfonate. Further studies on hydroacylation of butanal with PFP-vinyl sulfonate led to functionalised β-ketosulfonates which undergo elimination to form an enone and can then undergo further conjugate addition in situ by nucleophiles. Conjugate addition was carried out using carbon, nitrogen, oxygen and phosphorus nucleophiles, providing a method of obtaining products which are challenging to make via hydroacylation of electron-rich alkenes. Decarbonylation of pivaldehyde to the t-butyl radical, via auto-oxidation, was optimised and the alkyl radical captured by a number of electron-deficient acceptors, providing a complementary method to current methods of t-butyl addition using metal reagents.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Santin, Jean-Jacques. "Conception mécanique d'un accouplement à roue-libre pour le moteur thermique d'un véhicule hybride parallèle thermique et électrique." Valenciennes, 2001. https://ged.uphf.fr/nuxeo/site/esupversions/5c97a50f-ddaa-4980-958f-ff84e3b9148e.

Повний текст джерела

Анотація:

Ce mémoire porte sur la conception d'un accouplement à roue-libre. Il est destiné à remplacer l'embrayage piloté d'un véhicule hybride parallèle " ZEV ", simple arbre, à boîte de vitesses pilotée. Le premier chapitre définit le véhicule hybride étudié. Il développe ses modes de fonctionnement et montre la nécessité d'isoler le moteur thermique du reste de la chaîne de traction, en fonction des conditions de roulage. Le chapitre deux présente et compare deux solutions possibles : l'embrayage à sec piloté et la roue-libre. La mise au point de la solution à roue-libre a nécessité d'étudier les vibrations de torsion dans la transmission. Un outil modulaire spécifique a été développé sous MATLAB SIMULINK. Il est présenté dans le chapitre trois. Enfin, le chapitre quatre montre comment cet outil a été employé en phase de conception et présente une solution constructive. Cet accouplement doit être implanté dans un véhicule hybride prototype réalisé conjointement par le LAMIH et PSA
This thesis deals with the design of a free-wheel clutch. This unit is intended to replace the automated dry single-plate clutch of a parallel hybrid car with thermal and electric powertrain. Furthermore, the car is a single shaft zero emission vehicle fitted with a controlled gearbox. Chapter one focuses on the type of hybrid vehicle studied. It shows the need to isolate the engine from the rest of the drive train, depending on the driving conditions. Chapter two presents and compares the two alternatives : automated clutch and free-wheel. In order to develop the free-wheel option, the torsional vibrations in the automotive drive line had to be closely studied. It required the design of a specific modular tool, as presented in chapter three, with the help of MATLAB SIMULINK. Lastly, chapter four shows how this tool was used during the design stage and specifies the way to build it. The free-wheel is then to be fitted to a prototype hybrid vehicle, constructed by both the LAMIH and PSA

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Gallage, Roshini Samanthi. "Approximation Of Continuously Distributed Delay Differential Equations." OpenSIUC, 2017. https://opensiuc.lib.siu.edu/theses/2196.

Повний текст джерела

Анотація:

We establish a theorem on the approximation of the solutions of delay differential equations with continuously distributed delay with solutions of delay differential equations with discrete delays. We present numerical simulations of the trajectories of discrete delay differential equations and the dependence of their behavior for various delay amounts. We further simulate continuously distributed delays by considering discrete approximation of the continuous distribution.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Lindström, Veronica. "Adrenocorticolysis Induced by 3-MeSO2-DDE : Mechanisms of Action, Kinetics and Species Differences." Doctoral thesis, Uppsala universitet, Ekotoxikologi, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8180.

Повний текст джерела

Анотація:

The DDT metabolite 3-methylsulphonyl-DDE (3-MeSO2-DDE) induces cell death specifically in the adrenal cortex of mice after a cytochrome P45011B1 (CYP11B1)-catalysed bioactivation. This substance is not only an environmental pollutant, but also a suggested lead compound for an improved chemotherapy of adrenocortical carcinoma (ACC). The aim of the thesis was to further investigate this compound in terms of kinetics, cell death mechanisms and species differences. The pharmacokinetics of 3-MeSO2-DDE and the current drug for ACC, o,p’-DDD, was studied during 6 months following a single dose in minipigs. The elimination was slower for 3-MeSO2-DDE than for o,p’-DDD, indicated by a lower clearance and longer t½ in plasma and subcutaneous fat. Both substances remained in fat tissue during the whole study period. Unlike o,p’-DDD, 3-MeSO2-DDE was retained also in liver. The adequacy of the murine adrenocortical cell line Y-1 was evaluated for studies of adrenotoxic compounds. The Y-1 cells proved to be an appropriate test system for future mechanism studies, since CYP-catalysed irreversible binding, inhibited corticosterone production induced by 3-MeSO2-DDE and o,p’-DDD were successfully demonstrated. Cell death of 3-MeSO2-DDE in the mouse adrenal cortex was implied to be necrotic. Early apoptotic signalling (i.e. up-regulation of caspase-9) was observed, although it seemed to be interrupted by ATP-depletion and anti-apoptotic actions by heat shock protein 70, resulting in lack of activation of caspase-3. Using cultured adrenal tissue slices, two not previously studied species were examined ex vivo regarding adrenal binding of 3-MeSO2-[14C]DDE. Binding was found in the hamster adrenal cortex and in assumed cortical cells in the medulla, while the guinea pig adrenal was devoid of binding. This emphasises the species specificity in bioactivation of 3-MeSO2-DDE. The thesis forms a basis for further investigations in the human adrenocortical cell line H295R and provides new knowledge of importance for toxicological risk assessment of 3-MeSO2-DDE.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Lindström, Veronica. "Adrenocorticolysis induced by 3-MeSO2-DDE : mechanisms of action, kinetics and species differences /." Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8180.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Leone, Andrea D. "Enantiomeric composition of Chiral pesticides in soil and air from the U.S. cornbelt region." Youngstown State University / OhioLINK, 1998. http://rave.ohiolink.edu/etdc/view?acc_num=ysu997192215.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Santos, Diego Pereira dos. "Espalhamento Raman intensificado pela superfície (SERS) no regime de detecção de uma molécula." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-03052013-083112/.

Повний текст джерела

Анотація:

Nesta tese foi estudado o espalhamento Raman intensificado pela superfície (SERS) em regime de detecção de uma molécula em eletrodo de prata ativado por ciclos de oxidação e redução. Neste regime, de baixas concentrações, são observadas intensas flutuações de intensidade SERS as quais foram controladas neste substrato pela aplicação de potencial ao eletrodo, o que foi associado a alterações na concentração de moléculas adsorvidas na superfície do eletrodo. Além da dependência com o potencial aplicado, foram estudadas através de simulações Monte Carlo, a contribuição nestas flutuações da constante de adsorção das moléculas, do número de \"hot spots\" (regiões de altas intensificações SERS) e do tipo de \"hot spot\" (em termos de eficiência para detecção de espectros de uma molécula). Através destas simulações foram verificadas flutuações de intensidade muito semelhantes às observadas experimentalmente. Além das flutuações de intensidade foram também observadas flutuações de intensidades relativas, como por exemplo, das relações de intensidades anti-Stokes/Stokes, as quais foram interpretadas segundo um modelo de ressonância, através do qual foi possível estimar as energias de ressonância nos \"hot spots\". Alguns dos resultados indicaram a contribuição de ressonâncias finas, as quais foram interpretadas como resultado de interferências entre ressonâncias de plasmon de superfície. Interferências como estas foram demonstradas através de simulações pelo método DDA (\"Discrete Dipole appoximation\") em modelos simples de \"hot spots\" formados por nanobastões de Au.
In this thesis it was studied surface-enhanced Raman scattering (SERS) at single-molecule detection on Ag electrode activated by oxidation and reduction cycles. At this low concentration limit it was observed strong SERS intensity fluctuations that were controlled by the applied potential to the electrode and this control was associated to changes in surface concentration of adsorbed molecules. Furthermore, it was studied through Monte Carlo simulations the influence of adsorption constant, number of \"hot spots\" (regions of high SERS enhancements) and type of \"hot pot\" (in terms of efficiency for single-molecule detection). With such simulations, it was verified fluctuations of SERS intensities very similar to experimental observations. Besides absolute intensity fluctuations, we also observed fluctuations of relative intensities as, for instance, the. anti-Stokes to Stokes intensity ratios. These fluctuations were interpreted according to a resonance model, which made possible the estimative of resonance energies at the SERS \"hot spots\". Some of these results indicated the existence of sharp resonances that were interpreted as a result of interferences among surface plasmon resonances, which were demonstrated through DDA (Discrete Dipole Approximation) simulations in simple models of \"hot spots\" formed by Au nanorods

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Asp, Vendela. "In Vitro Studies of Adrenocorticolytic DDT Metabolites, with Special Focus on 3-methylsulfonyl-DDE." Doctoral thesis, Uppsala universitet, Ekotoxikologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122721.

Повний текст джерела

Анотація:

The DDT metabolite 3-methylsulfonyl-DDE (3-MeSO2-DDE) is bioactivated by cytochrome P450 11B1 (CYP11B1) in the adrenal cortex of mice and forms irreversibly bound protein adducts, reduces glucocorticoid secretion, and induces cell death selectively in cortisol-producing adrenocortical cells. 3-MeSO2-DDE has therefore been proposed as a lead compound for an improved adrenocortical carcinoma (ACC) therapy. The aims of this thesis were to (1) develop in vitro test systems based on murine and human adrenocortical cell lines and to (2) investigate the mechanisms behind 3-MeSO2-DDE toxicity in adrenocortical cells. The cytotoxic and endocrine-modulating effects of 3-MeSO2-DDE were compared to those of o,p′-DDD (mitotane), the current ACC therapy, and to those of several structurally analogous compounds in both murine and human cell lines. 3-MeSO2-DDE bioactivation and cytotoxicity proceeded in a similar manner in the murine adrenocortical Y-1 cell line as in mice in vivo. The effects were highly structure-specific. Moreover, 3-MeSO2-DDE formed irreversibly bound protein adducts and caused cell death also in the human H295R cell line, and was slightly more cytotoxic than o,p′-DDD. However, 3-MeSO2-DDE toxicity in human cells was not affected by the CYP11B1 inhibitor etomidate, suggesting that bioactivation in human cells is performed by additional/other enzyme(s) than CYP11B1. 3-MeSO2-DDE generated biphasic responses in cortisol and aldosterone secretion and in expression levels of the steroidogenic genes CYP11B1, CYP11B2, and StAR. Such hormesis-like responses were not seen for o,p′-DDD or the precursor DDT metabolite p,p′-DDE. In addition, the two o,p′-DDD enantiomers (R)-(+)-o,p′-DDD and (S)-(-)-o,p′-DDD exhibited slight differences in cytotoxic and endocrine-modulating activity in H295R cells. In conclusion, this thesis provides extended knowledge on the mechanisms of action of 3-MeSO2-DDE and points out important differences in effects between murine and human cells. Lead optimisation studies of 3-MeSO2-DDE using the herein presented in vitro test systems are ongoing.

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Vo, Phuong-Thao Thi Richardson David Barrie. "DDE and PCBs intra-individual changes, correlations, predictors and role in timing of menopause /." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1399.

Повний текст джерела

Анотація:

Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.
Title from electronic title page (viewed Apr. 25, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Epidemiology." Discipline: Epidemiology; Department/School: Public Health.

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Dufresne, Alain. "Etude d'interface / interphase de composites modeles dgeba-dda / billes de verre par fluage thermostimule." Toulouse, INSA, 1991. http://www.theses.fr/1991ISAT0006.

Повний текст джерела

Анотація:

La spectroscopie par fluage thermostimule a permis l'etude des deux principaux modes de retard de composites modeles bille de verre/matrice polyepoxy dans le domaine de temperature 180c/+150c. La microstructure des composites a ete comparee a celle du reseau utilise comme matrice. L'influence de la presence du renfort et de son traitement de surface a ainsi pu etre isolee et discutee en termes d'interphase et de mobilite moleculaire. Dans le domaine vitreux, une etude de la structure fine du spectre de modes de retard a montre le caractere complexe du mode basse temperature et l'existence d'une zone d'influence autour du renfort a ete mise en evidence. L'etude du mode haute temperature a permis d'estimer l'epaisseur de cette interphase. La resolution experimentale de ce mode a montre des phenomenes caracteristiques de l'interface/interphase. Le nombre de configurations possibles et la longueur des sequences mobiles ont pu etre compares a ceux de la matrice polyepoxy sans renfort. La technique du fluage thermostimule se revele donc particulierement bien adaptee a l'etude fine de la microstructure des composites a matrice organique

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Clouet, Johann. "Développement de l'ingénierie tissulaire du disque intervertébral : de la physiopathologie aux modèles animaux." Nantes, 2010. http://archive.bu.univ-nantes.fr/pollux/show.action?id=8c0865ed-dda5-4ac1-be66-9ee5be04f8f3.

Повний текст джерела

Анотація:

Les douleurs lombaires touchent 80% de la population au moins une fois dans la vie et constituent un véritable problème de santé publique pour nos sociétés modernes industrialisées. Ces douleurs lombaires sont, dans la grande majorité des cas, la conséquence clinique d'une atteinte des articulations vertébrales marquée par une dégénérescence progressive des disques intervertébraux. La connaissance des mécanismes menant à cette dégénérescence discale sont aujourd’hui de mieux en mieux connus et laisse envisager une prise en charge étiologique, et non plus uniquement symptomatique. Les premiers résultats concluants en ingénierie tissulaire du cartilage articulaire associés à l'existence de points communs entre le cartilage articulaire et le disque intervertébral laissent suggérer la possibilité de régénérer ce dernier, et en particulier le Nucleus pulposus (élément central du disque intervertébral), par ce même type d'approche. Le principe de cette approche est basé sur l'utilisation de cellules associées à un biomatériau. L'ensemble est injecté au sein d'un disque dégénéré afin de le restaurer. Un état des avancées actuelles dans ce domaine est réalisé et les différentes problématiques rencontrées au cours de la mise en œuvre de ce type de projet sont abordées. Il s'agit notamment du choix des cellules à injecter et des matrices associées, des conditions de culture adéquates, ainsi que du choix des méthodes d'évaluation et des modèles animaux
Low back pain affects 80% of the population at least once during life and constitutes a public health problem for our modern industrialized societies. Usually, they are the consequences of the intervertebral disc degeneration. Currently, the knowledges about mechanisms leading to this disc degeneration are well understood and allow to define new targets to treat the origin of the intervertebral disc degeneration. The first promising results in tissue engineering of articular cartilage associated with the existence of similarities between articular cartilage and intervertebral disc allow to considered the same approach to treat the intervertebral disc. The principle of this approach is based on the use of cells associated with a biomaterial and the substitute is injected into the degenerated disc. An update of current advances in this area is achieved and the various problems encountered during the development of such projects are discussed. These include the choice of cells and scaffolds injected, the choice of appropriate culture conditions, and the choice of evaluation methods and reliable animal models

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Yang, Yin, and 楊茵. "Characterization of DDA1, a p53-regulated gene." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/06664281071119188252.

Повний текст джерела

Анотація:

碩士
國立陽明大學
生物化學研究所
87
Wild type p53 expressed from a temperature-sensitive (tsp53) construct induces both G1 cell cycle arrest and apoptosis in the p53-negative IW32 mouse erythroleukemia cell line. Using PCR-based differential display analysis, we previously identified a new p53-inducible gene, DDA1, whose mRNA was upregulated in tsp53-transfected IW32 cells following induction of wild type p53 expression by temperature shift to 32°C. The DDA1 mRNA induction was detectable within 1 hour after temperature downshift, and rapid degradation was observed when the temperature was shifted back to 37°C, suggesting that the expression of DDA1 is dependent on the continuous presence of p53. The DDA1 mRNA was also induced in DNA damaging reagent-treated NIH3T3 cells. Previous studies from this lab have shown that mouse DDA1 cDNA predicted to encode a protein of 498 amino acid residues containing 12 transmembrane domains. The loop region between 6th and 7th transmembrane domains was used as immunogen to produce rabbit polyclonal antibodies, and antibodies that can recognize E. coli expressed mDDA1 protein was obtained. Immunofluorescence analysis indicated that DDA1 protein is located in the cytoplasm. Overexpression of mDDA1 cDNA in H1299 cells inhibited cell growth，as shown by the colony formation assay. The cDNA of human DDA1 that is 73 % identical to mouse DDA1 was acquired by library screening and database searching. The two amino acid sequences share 90 % identity.

Стилі APA, Harvard, Vancouver, ISO та ін.

47

CHIAHUIWU and 吳佳惠. "Characterization of a p53-regulated gene DDA1." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/64211439442454738164.

Повний текст джерела

Анотація:

碩士
國立陽明大學
生物化學研究所
88
Abstract p53 tumor suppressor is a transcription factor that causes cell growth arrest and induces apoptosis. Identification of the p53 downstream target genes is therefore important to unravel the mechanisms underlying p53 actions. We have previously identified and cloned a p53-regulated gene, DDA1, by the RNA differential display of an IW32 erythroleukemia stable clone (1-5) that contains a temperature-sensitive p53 mutant gene, tsp53val135. Sequence comparison revealed that mDDA1 shares 73% and 90% identity in its nucleotide and protein sequences, respectively, to the newly identified human thiamine transporter gene (hTHTR-1). To further investigate the subcellular localization and function of DDA1, we established DDA1 expressing clones under the control of the tetracycline inducible promoter. After 24 hours treatment of 2 mg /ml Doxycycline, clone 118 transfectant cells could be induced to express DDA1 mRNA and protein. Immunofluorescence analysis indicated that DDA1 was present on the plasma membrane. Growth of DDA1 stable transfectant was partly inhibited in the presence of Doxycycline. Ability of the clone 118 cells to uptake thiamine increased 2-fold in the presence of Doxycycline. These data demonstrated that mDDA1 possesses thiamine transporter activity. hTHTR-1 mRNA was induced by DNA damage in a p53 dependent manner. Induction was detected in 293 cells expressing endogenous p53, but not in 293T cells whose p53 was inactivated. Together these results indicate that the high affinity thiamine transpoter is a p53 regulated gene. 中文摘要 ..................................................... 1 英文摘要 ..................................................... 2 緒論 ..................................................... 3 實驗材料 ..................................................... 16 實驗方法 ..................................................... 18 實驗結果 ..................................................... 30 實驗討論 ..................................................... 36 參考文獻 ..................................................... 40 附圖 ..................................................... 47

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Hsin, Jin-Ping, and 邢金平. "Studies of the p53 regulated gene DDA3." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/83222073647311780238.

Повний текст джерела

Анотація:

碩士
國立陽明大學
生物化學研究所
86
p53為一抑癌基因，當細胞DNA損害時p53常會大量表現導致G1 arrest或apoptosis。由本實驗室所建立的可持續表現受溫度調控的p53 mutant的轉型細胞株1-5，利用而tNA差異展現法(differential display)我們選殖到可受p53調控的基因mDDA3。經DNA序列分析，發現血DDA3的cDNA全長為1.8kb。再由human EST database找到了一個EST clone將其定序並與老鼠的DDA3比對後，發現二者之胺基酸序列具有67.9%的相似性，並且皆含3個SH3 binding motif PXXP。Colony的rmation assay結果顯示DDA3大量表現可以抑制H1299人類肺癌細胞的生長。將mDDA3的N端接上FLAGpeptides，以immunofluorescence觀察可見而JDA3在細胞質中表現。在invitro及in vivo下做轉錄和轉譯，可得大小約39kD和43kD的蛋白，這和由DDA3的open reading frame所預測出的蛋白質大小接近。利用human及mouse DDA3的相似區域我們設計了二條peptides，將其與BSA結合後注射兔子可以測得血清中有抗體。至於其與DDA3蛋白之結合，則有待更進一步的實驗確認。 p53 tumor suppressor is a transcription factor that functions through activation of a number of downstream target genes. We have previously identified and cloned a p53 regulated gene, DDA3, through differential display of the mRNAs of the IW32 mouse erythroleukemia cells grown in the presence or absence of wild type p53. The mouse DDA3 cDNA (mDDA3) is about 1.8 kb in size. Two alternatively spliced transcripts were cloned, mDDA3s contains an open reading frame of 329 amino acids and mDDA3L has an additional ATG at 45 bp upstream of the translation initiation codon of mDDA3s. Through homology search of the human EST database, an EST clone was identified and sequenced. This cDNA showed 69% identity in protein sequence with mDDA3, and may represent the human homologue of mDDA3. In vitro transcription and translation showed that mDDA3 and hDDA3 could both encode proteins of 39kDa in size. When mDDA3 was fused to the Flag tag at its 5' terminus and introduced into cells, a protein of 43kDa that reacted with anti-Flag antibody was found. Immunofillorescence indicates that DDA3 was distributed in the cytosol. Over-expression of DDA3 suppressed the growth of H1299 human non-sinall-cell lung cancer cells, as analyzed by colony formation assay. These results suggest that DDA3 may mediate, at least in part, the growth suppressing function of p53.

Стилі APA, Harvard, Vancouver, ISO та ін.

49

Yuan, Chen Sheng, and 陳勝源. "The Preliminary Study of Rockfall Simulation using DDAD." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/35019879865550013325.

Повний текст джерела

Анотація:

碩士
中原大學
土木工程研究所
86
Many engineering structures near the mountain region of Taiwanhave been subjected to the threat of rock-fall. In order to economically and efficiently install protection means, identificationof rock-fall source area as well as prediction of falling rock pathsis essential. In this thesis, the ROCKPATH program was written to analyze the behavior of a single falling rock, and the DDAD code was adopted to study the effects of multiple falling rocks. Taiwan is located between Euro-Asia and Philippine plates, and two-thirds of its area is covered by mountains. Because of local geological and climate conditions, landslides of different types occurs frequently, and among them, rock-fall is more typical. In literature, many rock-fall simulation programs were developed by foreign scholars and were merely capable of handling one single falling rock, neglecting the effects of multiple falling rocks. Based on the lumped-mass method for a single falling rock, ROCKPATH was developed, and its validity was verified by several illustrative examples. As a special version of the Discontinuous Deformation Analysis for Disks, DDAD can analyze the complex interactions of thousands of particles. The task of modifying the velocity components of a particle after impacting a slope enabled DDAD to simulate rock-fall problems, and DDAD simulation results for single falling rock examples were well comparable to those computed by ROCKPATH. In the additional three examples provided in this thesis, DDAD computation results revealed that the effects of multiple falling rocks could not be ignored and the protection design based on single falling rock mode would not be conservative. Moreover, the normal restitution coefficient was back-calculated by DDAD for a falling rock case in Taiwan.

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Hsieh, Pei-Chen, and 謝佩真. "Functional analysis of DDA3, a p53 downstream target." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/70556467797681446541.

Повний текст джерела

Анотація:

博士
國立陽明大學
生化暨分子生物研究所
95
We have previously identified mouse DDA3 as a p53-inducible gene. To explore the functional role of DDA3, we screened a mouse brain cDNA library by the yeast two-hybrid assay, and identified the microtubule plus-end binding protein EB3 as a DDA3 interacting protein. Binding of DDA3 to EB3 was verified by GST pull-down assay and subcellular co-localization; co-immunoprecipitation further indicated that interaction of these two proteins within cells required intact microtubules. Domains of DDA3-EB3 interaction were mapped by GST pull-down assay to amino acids (a.a.) 118-241 and 242-329 of DDA3 and the N- and C-termini of EB3. Immunofluorescence analysis revealed co-localization of DDA3 with microtubules in various cell phases, and regions encompassing a.a. 118-241 and 242-329 contained microtubule-interacting and bundling activities. In vitro microtubule binding assay showed that DDA3 and EB3 associated directly with microtubules, and cooperated with each other for microtubule binding. In addition, DDA3 bound to the EB3 interacting partner APC2, a homologue of the tumor suppressor adenomatous polyposis coli (APC) which is a component of the β-catenin destruction complex. Ectopic expression of DDA3 and EB3 enhanced β-catenin-dependent transactivation and cyclin D1 production, while knockdown of endogenous DDA3 or EB3 inhibited β-catenin-mediated transactivation and the ability of cells to form colonies, implicating DDA3 and EB3 in the β-catenin-mediated growth signaling. Furthermore, overexpression of DDA3 inhibited neurite formation during NGF-induced PC12 differentiation, whereas knockdown of DDA3 expression by small interference RNA resulted in neurite outgrowth in N2a cells. Consistent with these findings, DDA3 expression was downregulated during retinoic acid induced differentiation of N2a neuroblastoma cells. Together, our results uncover novel roles of DDA3 in microtubule dynamics, cell growth and neuronal differentiation.

Стилі APA, Harvard, Vancouver, ISO та ін.

Дисертації з теми "DDAE"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями