Дисертації з теми "Cytochrome P45Os"
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Stok, Jeanette Elizabeth. "Biosynthetic cytochrome P450s /." [St. Lucia, Qld.], 2001. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16210.pdf.
Повний текст джерелаOrr, Catherine. "Characterisation of equine cytochrome P450s." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33315/.
Повний текст джерелаRylander, Rudqvist Tove. "Extrahepatic cytochrome P450s : relation to cancer susceptibility /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-601-4/.
Повний текст джерелаKusimo, Michael Olugbenga. "Characterisation of cytochrome P450s in Anopheles gambiae." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/9313/.
Повний текст джерелаHodgkinson, Conrad Phillip. "Expression of cytochrome P450s in rat hepatocyte culture." Thesis, Queen Mary, University of London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244085.
Повний текст джерелаGillett, Lorna. "Function of cytochrome P450s in the CYP4 family." Thesis, University of Nottingham, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408051.
Повний текст джерелаPineau, Emmanuelle. "Formation des acides gras poly-hydroxylés et incorporation dans la cutine chez Arabidopsis thaliana." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ052/document.
Повний текст джерелаPlants are sessile organisms that are not able to escape from difficult environmental conditions and therefore have to adapt to multiple abiotic and biotic stress to survive. Cutin is a part of the cuticle which plays a major role as a barrier for the plant. It’s a lipid polymer composed mainly by hydroxylated and epoxidized C16 and C18 fatty acids linked together by ester links involving the carboxyl and ω-hydroxyl functions of those fatty acids. Cutin plays also a role as a reservoir of molecules with fundamental physiological properties. With biochemical and genetic approaches, we characterized AtEH1, an epoxide hydrolase responsible for the formation of diols incorporated in Arabidopsis thaliana cutin. These diols are described as being involved in plant-pathogen interactions. We also showed that these compounds as well as others fatty acids derivatives are perceived by plants. We have also identified and characterized CYP77B1, an epoxidase that has a potential role in the formation of polyhydroxylated fatty acids incorporated in cutin
Hunter, Arwen Leigh. "The role of peri-transplant ischemia and reperfusion injury in cardiac allograft vasculopathy." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/2503.
Повний текст джерелаRosic, Nedeljka. "Molecular breeding of cytochrome P450s for indigoid pigment production /." [St. Lucia, Qld.], 2005. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18978.pdf.
Повний текст джерелаDodhia, Vikash Rajnikant. "Engineering human cytochrome P450s for structural and biosensing studies." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429524.
Повний текст джерелаDavies, Lindsay. "Molecular cloning and characterization of cytochrome P450s metabolising ecdysteroids." Thesis, University of Liverpool, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269568.
Повний текст джерелаNisbar, Nur Dayana Binti. "Characterisation of orphan cytochrome P450s from Mycobacterium tuberculosis H37Rv." Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-orphan-cytochrome-p450s-from-mycobacterium-tuberculosis-h37rv(5f953958-9722-4531-b8b2-b034adaaabb0).html.
Повний текст джерелаBasom, Edward J. "Dynamics and Conformational Heterogeneity in Cytochrome P450s via Infrared Spectroscopy." Thesis, Indiana University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10604874.
Повний текст джерелаCytochrome P450s (P450s) are a superfamily of enzymes that catalyze oxidation of unactivated hydrocarbons. However, the means by which P450s control (1) regioselectivity of their activity and (2) specificity in their molecular recognition remain largely elusive. Toward investigation of the role of dynamics in the regioselectivity of the archetypal cytochrome P450cam (P450cam), two-dimensional infrared spectroscopy has been applied with heme-bound carbon monoxide (CO) as an infrared probe of the active site. The data support a model for P450cam regioselectivity in which binding of different substrates to P450cam variably stabilizes the active site into two distinct states, each associated with different dynamics linked to different levels of regioselectivity. To investigate the role of conformational heterogeneity in P450cam substrate specificity, infrared spectoscopy was combined with the site-specific incorporation of nitrile probes at distinct P450cam microenvironments. This approach enabled differentiation of changes experienced at each of those environments when d-camphor and/or CO binds to the active site. Finally, the impact of conformational heterogeneity on the affinity of substrate molecular recognition by wild-type and mutant P450cam was evaluated using both CO and nitrile probes. This study suggests that the nature of the conformations populated in the unbound states influences the affinity for different substrates. Collectively, these studies provide new insight into the roles of conformational heterogeneity and dynamics in P450cam activity. Furthermore, these studies help to lay the foundation for efforts toward understanding the roles of conformational heterogeneity and dynamics in the function of human P450s, for which unraveling the mechanisms involved in Phase I metabolism is a topic of great pharmacological concern.
Hahn, Christopher Norman. "Regulation of chicken cytochrome P450s and 5-Aminolevulinic acid synthase." Title page, contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09PH/09phh148.pdf.
Повний текст джерелаBrundage, Meghan Elizabeth. "CLONING OF KNOWN AND NOVEL CYTOCHROME P450S IN SCUTELLARIA BAICALENIS." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin998483270.
Повний текст джерелаTrnka, Michael J. "Photoaffinity labeling of cytochrome P450s with imidazole-tethered benzophenone compounds /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/8516.
Повний текст джерелаRichmond, Alison. "Expression and characterisation of biomedically and biotechnologically important bacterial cytochrome P450s." Thesis, University of Strathclyde, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275059.
Повний текст джерелаIng, Rachael Helen. "Enzymology and induction of hepatic cytochrome P450s in the guinea pig." Thesis, De Montfort University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393360.
Повний текст джерелаChangenet, Valentin. "Towards new roles for cytochrome P450s and strigolactones in Fusarium Head Blight of Brachypodium distachyon." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS354/document.
Повний текст джерелаFusarium Head Blight (FHB) is one of the most important diseases of temperate cereals and is mostly caused by the toxin producing-fungus Fusarium graminearum (Fg). This last decade, several studies reported the transcriptional activation of cereal cytochrome P450-encoding genes (P450s) in response to Fg infection. P450s constitute an enzymatic family participating in very diverse metabolic pathways with potential interest for disease resistance. We used the model temperate cereal Brachypodium distachyon (Bd) to functionally characterize the first FHB-induced P450- encoding gene using Bd lines altered in the locus or gene expression of the Bradi1g75310 gene encoding the BdCYP711A29 P450. We showed that in addition to be a plant susceptibility factor towards the disease, the Bradi1g75310 gene is involved in the hormonal biosynthetic pathway of strigolactones (SLs) in Bd. Indeed, in addition to genetically complement the shoot phenotypes of the Arabidopsis thaliana mutant line for the homologous gene MAX1 (AtCYP711A1, max1-1 line), a Bd linewhich overexpresses the Bradi1g75310 gene (OE) exudes more orobanchol, a specific SL, compared to wild-type or mutant lines. Preliminary analysis of the direct impact of orobanchol on Fg growth suggests an activation of early fungal development (germination) likely to induce faster induction of defense-related genes during FHB, observed in Bradi1g75310 OE line. We showed that the four paralogs of Bradi1g75310 encoding BdCYP711A P450s are all able to genetically complement max1-1 line and provide important plant material for studying SLs diversification in the model monocot B. distachyon. Overall, this project constitutes a first step in the characterization of P450s involvement in plant response towards Fg infection in addition to give new evidences about the role of SLs in plant-pathogen interactions. Results obtained during this Ph.D. project will allow the improvement of both developmental and FHB-related traits in cereal crops
Nikou, Dimitra. "Molecular analysis of multiple cytochrome P450s from the maleria vector Anopheles gambiae." Thesis, University of Liverpool, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275044.
Повний текст джерелаWithers, John C. "CHARACTERIZATION OF TWO NOVEL CYTOCHROME P450S INVOLVED IN GRAVITROPISM IN ARABIDOPSIS THALIANA." Ohio University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187184960.
Повний текст джерелаZhu, Shiqian. "The roles of cytochrome P450s in the toxicity of polycyclic aromatic hydrocarbons (PAHs) /." Full text available from ProQuest UM Digital Dissertations, 2007. http://0-proquest.umi.com.umiss.lib.olemiss.edu/pqdweb?index=0&did=1800288781&SrchMode=1&sid=12&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1258491890&clientId=22256.
Повний текст джерелаYadav, Jaydeep. "EVALUATING PHARMACOKINETIC DRUG-DRUG INTERACTIONS DUE TO TIME DEPENDENT INHIBITION OF CYTOCHROME P450s." Diss., Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/524248.
Повний текст джерелаPh.D.
Time-dependent inactivation (TDI) of CYPs is a leading cause of clinical drug-drug interactions (DDIs). Current methods tend to over-predict DDIs. In this study, a numerical approach was used to model complex CYP3A TDI in human liver microsomes. Inhibitors evaluated include troleandomycin (TAO), erythromycin (ERY), verapamil (VER), Paroxetine (PAR), itraconazole (ITZ) and diltiazem (DTZ) along with primary metabolites N-demethyl erythromycin (NDE), norverapamil (NV), and N-desmethyl diltiazem (MA). Complexities incorporated in the models included multiple binding kinetics, quasi-irreversible inactivation, sequential metabolism, inhibitor depletion, and membrane partitioning. The different factors affecting TDI kinetics were evaluated such as lipid partitioning, inhibitor depletion, presence of transporters. The inactivation parameters obtained from numerical method were incorporated into static in-vitro – in-vivo correlation (IVIVC) models to predict clinical DDIs. For 123 clinically observed DDIs, using a hepatic CYP3A synthesis rate constant of 0.000146 min-1, the average fold difference between observed and predicted DDIs was 2.97 for the standard replot method and 1.66 for the numerical method. Similar results were obtained using a synthesis rate constant of 0.00032 min-1. These results suggest that numerical methods can successfully model complex in-vitro TDI kinetics and that the resulting DDI predictions are more accurate than those obtained with the standard replot approach. Chapter one presents the detailed introduction along with the hypothesis and significance of the project. Chapter 2 includes the development of the bioanalytical method for quantitation of various compounds which includes inactivators and their primary metabolites. Chapter 3 entails the discussion on in-vivo studies in rats involving TDI mediated DDI studies. Chapter 4 discusses the in-vitro studies and use of the numerical method for evaluation of TDI kinetics. Chapter 5 and chapter 6 provides discussion on the impact of inhibitor depletion and partitioning of TDI kinetics and how these two could lead to misinterpretation of TDI results. Chapter 6 also provides a discussion on how transporters could affect TDI results mainly from hepatocyte studies. Chapter 7 involves prediction of TDI mediated DDI using static modeling. Chapter 8 is a case study on bosentan involving induction mediated DDI.
Temple University--Theses
Gravot, Antoine. "Étude de P450s impliqués dans la biosynthèse des furocoumarines chez Ruta graveolens." Vandoeuvre-les-Nancy, INPL, 2002. http://www.theses.fr/2002INPLA63N.
Повний текст джерелаAl-Nuaemi, Inas. "Investigating the industrial application potentials of cytochrome P450 BM-3 and four novel putative cytochrome P450s isolated from Cupriavidus necator H16." Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/22435/.
Повний текст джерелаChenge, Jude. "Investigating orphan cytochrome P450s in Mycobacterium tuberculosis : insights into enzyme structure, function and inhibitor design." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/investigating-orphan-cytochrome-p450s-in-mycobacterium-tuberculosis-insights-into-enzyme-structure-function-and-inhibitor-design(2965ac43-d907-41d8-8222-1f327dc745e4).html.
Повний текст джерелаXu, Haibo. "Effects of human milk and formula on the expression of cytochrome P450s in cell lines." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ63179.pdf.
Повний текст джерелаBateson, Hannah. "Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques : development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research." Thesis, University of Bradford, 2012. http://hdl.handle.net/10454/5712.
Повний текст джерелаSwami, Shalini. "Structure and biochemistry of the orphan cytochrome P450s CYP126A1 and CYP143A1 from the human pathogen Mycobacterium tuberculosis." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/structure-and-biochemistry-of-the-orphan-cytochrome-p450s-cyp126a1-and-cyp143a1-from-the-human-pathogen-mycobacterium-tuberculosis(a8dc4eea-678c-419a-a3b3-206253c400b7).html.
Повний текст джерелаMohamed, Hanafy Mahmoud Ismail. "Developing novel pyrethroid activity based probes to identify cytochrome P450S associated with pyrethroid resistance in disease vectors." Thesis, University of Liverpool, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.548796.
Повний текст джерелаSmith, Susan James. "A resonance Raman and surface enhanced resonance Raman study of cytochrome P450s and their substrate/inhibitor interactions." Thesis, University of Strathclyde, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288604.
Повний текст джерелаJEAN, PASCALE. "Mecanisme de l'inhibition des cytochromes p450s hepatiques par les 2-aroyl thiophenes et modelisation moleculaire (doctorat : toxicologie)." Paris 5, 1997. http://www.theses.fr/1997PA05N137.
Повний текст джерелаXu, Yun. "Mechanistic studies on the differences between in vitro and in vivo inhibition potencies of fluvoxamine towards various cytochrome P450s /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/7972.
Повний текст джерелаLOUERAT, ORIOU BENEDICTE. "Purification et caracterisation des nadph-cytochromes p450 reductases humaine, de plante et de levure, et analyse de leurs couplages avec les formes purifiees des p450s 1a et 2b." Paris 5, 1998. http://www.theses.fr/1998PA05N116.
Повний текст джерелаCompagnon, Vincent. "Régulation de l'expression et rôles physiologiques de cytochromes P450s catalysant l'hydroxylation d'acides gras chez Vicia sativa et Arabidopsis thaliana." Université Louis Pasteur (Strasbourg) (1971-2008), 2006. https://publication-theses.unistra.fr/restreint/theses_doctorat/2006/COMPAGNON_Vincent_2006.pdf.
Повний текст джерелаOmega-hydroxylated fatty acids play diversified roles in the plant : they constitute monomers of the plant polyesters cutin and suberin, second messengers in plant-pathogen interactions, and are intermediates of the fatty acids catabolism. Cytochrome P450 isoforms from CYP86 and CYP94 families could be implicated in these reactions. The identification of some of these isoforms is the aim of our work. In Vicia sativa, high concentrations (50 to 500 µM) of chemicals (clofibrate, 2,4-D, 2,3-D, IAA, SA, methyljasmonate) applied on the seedlings produce a strong induction of CYP94A1. A promotor analysis revealed the occurrence of an as-1 regulatory sequence, which, as in the regulation of isoform 6 of the glutathione-S-transferase, would suggest a role in the detoxification of free fatty acids. CYP86B1 and CYP86B2 from Arabidopsis thaliana are involved in the biosynthesis of suberin monomers in the root endodermis, and probably in the production of lipids for the pollen coat and the wall of pollen grains
Davies, Adrian M. "Genotoxicity of tamoxifen and structural analogues of tamoxifen : studies on the activation of tamoxifen by cytochrome P450s and peroxidase." Thesis, University of Leicester, 1999. http://hdl.handle.net/2381/29637.
Повний текст джерелаShepard, Dale Randall. "The Metabolism of Phenytoin by Human Liver Microsomes and Cytochrome P450s Expressed in Saccharomyces Cerevisiae and COS-1 Cells /." The Ohio State University, 1995. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487931512618872.
Повний текст джерелаLee, Sungbeom. "EXPLORING THE BIOCHEMICAL AND EVOLUTIONARY DIVERSITY OF TERPENE BIOSYNTHETIC ENZYMES IN PLANTS." UKnowledge, 2008. http://uknowledge.uky.edu/gradschool_diss/587.
Повний текст джерелаAbdulmughni, Ammar [Verfasser], and Rita [Akademischer Betreuer] Bernhardt. "Engineering of Cytochrome P450s CYP109E1 and CYP109A2 from Bacillus megaterium DSM319 for the production of vitamin D3 metabolites / Ammar Abdulmughni ; Betreuer: Rita Bernhardt." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2018. http://d-nb.info/1166140032/34.
Повний текст джерелаAbdulmughni, Ammar Verfasser], and Rita [Akademischer Betreuer] [Bernhardt. "Engineering of Cytochrome P450s CYP109E1 and CYP109A2 from Bacillus megaterium DSM319 for the production of vitamin D3 metabolites / Ammar Abdulmughni ; Betreuer: Rita Bernhardt." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2018. http://d-nb.info/1166140032/34.
Повний текст джерелаIbrahim, Sulaiman Sadi. "Allelic variation and multigenic metabolic activity of cytochrome P450s confer insecticide resistance in field populations of Anopheles funestus s.s., a major malaria vector in Africa." Thesis, University of Liverpool, 2015. http://livrepository.liverpool.ac.uk/2008567/.
Повний текст джерелаCrocoll, Christoph [Verfasser], Jonathan [Akademischer Betreuer] Gershenzon, Christian [Akademischer Betreuer] Hertweck, and Harro [Akademischer Betreuer] Bouwmeester. "Biosynthesis of the phenolic monoterpenes, thymol and carvacrol, by terpene synthases and cytochrome P450s in oregano and thyme / Christoph Crocoll. Gutachter: Jonathan Gershenzon ; Christian Hertweck ; Harro Bouwmeester." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2011. http://d-nb.info/1016391315/34.
Повний текст джерелаFraser, Emily Anne. "Investigating the role of CAR and PXR in the regulation of cytochrome P450s and other drug metabolising enzymes by anti-cancer drugs using novel humanised mouse models." Thesis, University of Dundee, 2013. https://discovery.dundee.ac.uk/en/studentTheses/53400dff-cf5e-49a6-99ca-9bd95b2326bd.
Повний текст джерелаSarkar, Md Raihan. "Application of the Monooxygenase Enzymes CYP101B1 and CYP101C1 from Novosphingobium aromaticivorans for Selective and Efficient Functionalisation of Inert C-H bonds." Thesis, 2019. http://hdl.handle.net/2440/119892.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Physical Sciences, 2019
Fu, Chung-Nan, and 傅正男. "Effect of Carbendazim on Cytochrome P450s in Rats." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/24962781166715390064.
Повний текст джерела國立臺灣大學
毒理學研究所
95
Carbendazim (methyl-2-benzimidazole carbamate) is a broad spectrum and systemic fungicide for the control of molds, rots, and blight. Carbendazim and related benzimidazoles show marked reproductive toxicity and endocrine-disrupting activity in rats. Cytochrome P450 (CYP) is the primary enzyme system in metabolism and is responsible for the metabolism of drugs, carcinogens, steroid hormones, and environmental pollutants. CYP genes are markedly responsive to the stimulatory and inhibitory effects of xenobiotics and provide a powerful tool to investigate gene-environment interaction. The major objective of the present study was to investigate the ability of carbendazim to modulate CYP-dependent monooxygenases and antioxidant enzymes in rat liver, kidney, lung, and testis. Treatment of male rats with 10, 50, and 100 mg/kg carbendazim intraperitoneally once daily for 4 days decreased testis spermatid density dose-dependently. In liver microsomes, the carbendazim treatment increased P450 content, NADPH-cytochrome c reductase, 7-ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), pentoxyrsorufin O-dealkylase, and 7-ethoxycoumarin O-deethylase (ECOD) activities. In kidney microsomes, carbendazim increased P450 content and EROD and ECOD activities. In lung microsomes, the treated increased EROD activity. In testis microsomes, the treatment increased NADPH-cytochrome c reductase activity. Carbendazim increased glutathione S-transferase (GST) and catalase activities in liver cytosol and GST and superoxide dismutase activities in testis cytosol. The fungicide decreased glutathione content in the kidneys and lipid peroxidation in the testes. Oral administration of 400 mg/kg carbendazim for 7 days increased MROD activity in liver microsomes. The results of immunoblot and RT-PCR analyses showed that carbendazim induced CYP1A1/2 and CYP2B proteins and mRNA in the liver and CYP1A1 protein and mRNA in kidneys and lungs. These present findings show that carbendazim is an inducer of CYP1A1/2 and CYP2B in rats. The significance of carbendazim induction of CYP in metabolic activation warrants further investigations.
Armstrong, Catherine. "Characterizing the Expression of Cytochrome P450s in Breast Cancer Cells." Thèse, 2011. http://hdl.handle.net/1866/9017.
Повний текст джерелаSeveral types of cancer cells have shown an innate or accute resistance to anti-cancer agents which in turn causes a failure in treatment. This resistance has been suggested to be caused by the expression of membrane transporters in cancer cells, as well as inter-individual variability in metabolism. Our interest was to evaluate the implication of CYP450 enzymes in the local metabolism of cancer cells. Our first objective was to screen the expression level of six housekeeping genes (HKG) using 23 different cell lines to determine which gene was the most stable. We found that NUP-214 was the most stable HKG across the panel of cell lines tested, with a standard deviation of only 0.55 Ct. Our second objective was to determine the expression level of 19 CYP450 mRNA isoforms in various breast cancer cell lines by RT-PCR. The CYP450 mRNAs showed a large variability between the different cell lines analyzed, where CYP1B1 and 2J2 were strongly expressed in most cell lines. Our third objective was to determine if measurable metabolic activity was present and correlates with mRNA expression in these same breast cancer cell lines using the specific substrates 7-ethoxyresorufin and ebastine for CYP1B1 and 2J2 activities, respectively. The metabolism of 7-ethoxyresorufin showed an excellent correlation of 0.98 with CYP1B1 expression while ebastine demonstrates a strong correlation (r2=0.99) with 2J2 expression. Overall, these results suggest that local metabolism of anti-cancer agents could significantly affect drug disposition and be a source of chemoresistance.
Munday, Samuel David. "Investigations and applications of self-sufficient cytochrome P450 monooxygenases." Thesis, 2016. http://hdl.handle.net/2440/100734.
Повний текст джерелаThesis (M.Phil.) -- University of Adelaide, School of Physical Sciences, 2016.
Chang, Chiao-Chia, and 張巧佳. "Role of Human Hepatic Cytochrome P450s in Territrem B and C Metabolism." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/78550460089862607345.
Повний текст джерела國立臺灣大學
毒理學研究所
93
Territrem A, B and C, the structure related tremogenic mycotoxins isolated from the chloroform extracts of rice culture of Aspergillus terreus 23-1. The previous study on metabolism of Territrem A (TRA) by liver microsome from Wistar rats showed that three metabolites, MA1, MAX and MA2 were formed in male rats, but only one metabolite, MA1 in female rats. The studies were further carried with specific chemical and antibody inhibitors for CYP isoforms and the sypersomes expressed with specific type of CYP isoforms. The results indicated that CYP3A1, which is dominated in female rat liver, plays a mean role in metabolic pathway from TRA to MA1 and that CYP3A2, which is dominated in male rat liver, plays the mean role in metabolic pathway of TRA to MA1, MAX and MA2. On the other hand, four metabolites, such as MB1, MB2, MB3 and MB4 (which structure is the same as Territrem C (TRC) ) were formed from Territrem B (TRB) and one metabolite, MC (which structure is the same as MB4) from Territrem C respectively by liver microsome from each of male and female rats. In order to elucidate the role of human hepatic CYP isoforms in TRB and TRC, the experiments were performed with several enzyme sources such as from different age and sex, V79MZh3A4 cell line derived from Chinese hamster, in which human CYP3A4 were expressed, and several supersomes having enzyme or different tyoe of CYP isoforms. The following are the resultes obtained: (1) In human liver microsome, MB2, and MB4 were main products from TRB and MC from TRC. There were no age and gender difference in ability to metabolize TRB and TRC. (2) CYP3A4/5 had the major role in metabolism of TRB and TRC. (3) The rate of production of MB2 from TRB or MC from TRC were competitively inhibited by the present of testosterone. However the rate of the production of 6β-hydroxytestosterone from testosterone were inhibited by the present of TRB or TRC in mixed type of inhibition (non competitive and non uncompetitive inhibition). The rate of the production of MB4 from TRB was also inhibited by the present of testosterone in mixed type of inhibition. (4) It suggested that CYP3A4 had the major role in 4β-C hydroxylation of TRB, and CYP3A5 had the major role in O-demethylation of TRB.
Behan, Rachel Koren. "Spectroscpic characterization of high-valent intermediates in Cytochrome P450s and other heme enzymes." 2008. http://etda.libraries.psu.edu/theses/approved/WorldWideIndex/ETD-2438/index.html.
Повний текст джерелаGreen, Robert. "Investigation of Cytochrome P450 Monooxygenases in S. homoeocarpa for Chlorothalonil Biotransformation." 2017. https://scholarworks.umass.edu/masters_theses_2/506.
Повний текст джерела