Дисертації з теми "Cysteine, N-acetyl Cysteine"
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KUO, MING-WEN. "ROLE OF N-ACETYL CYSTEINE IN PREVENTING AGE-RELATED HEARING LOSS." University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1172356377.
Повний текст джерелаKaralija, Amar. "Diagnostic and therapeutic strategies following spinal cord and brachial plexus injuries." Doctoral thesis, Umeå universitet, Anatomi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-127519.
Повний текст джерелаCheng, Huiwen. "Effects of N-acetyl-L-cysteine and Nitric Oxide-releasing Nonsteroidal Anti-Inflammatory Drugs on Breast Cancer and Melanoma Cell Adhesion." Ohio University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1384948906.
Повний текст джерелаMoore, Thomas B. "The Role of N-acetyl-L-Cysteine (NAC) as an Adjuvant to Opioid Treatment in Patients with Inadequately Controlled Chronic Neuropathic Pain." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4315.
Повний текст джерелаRealini, Giulia. "N-acetyl-L-cysteine ethyl ester (NACET) as a potential therapeutic strategy for the prevention and treatment of Age-related Macular Degeneration." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1203143.
Повний текст джерелаSchenatto, Ricardo Olimpio. "Uso da N-acetil-L-cisteína no resfriamento de sêmen equino." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/181359.
Повний текст джерелаThe aim of this study was to evaluate the effect of N-acetyl-L-cysteine (NAC) based on skim milk powder extender on sperm viability and oxidative stress of equine semen cooled to 5°C. Ejaculate of 8 ponies of the Brazilian breed were collected in triplicate, resulting in 24 ejaculates, distributed in 4 groups: Equidil® extender + 0.00mm (control), 0.5mM, 1.0mM and 2.5mM of NAC with the semen samples were The samples were stored in 15mL tubes and kept in BotuFLEX® semen transport boxes (Botupharm, Botucatu/SP/Brazil). Parameters, such as motility, vigor, pH, osmolarity, HOST, and MTT were evaluated at 24 and 48 h of cooling also in fresh semen. Vigor, total (TM) and progressive motility (PM) were also evaluated after thermoresistance test (TTR), in the absence and presence of peroxide. TM, PM, sperm vigor and MTT were similar (P > 0.05) between NAC concentrations at 24 and 48 h. The response to HOST was similar between NAC concentrations (P > 0.05) at 24 h cooling, but at 48 h there was a decreased in membrane functionality in 2.5mM NAC group compared to the EQUIDIL group. TM, PM, and vigor of the samples cooled by 24 h and submitted to TTR differed between without and with peroxide (P< 0.05) in the EQUIDIL, 0.5mM and 1.0mM groups, but was similar in 2.5mM NAC. After cooling for 48 h, there was difference in vigor and TM between samples with and without peroxide (P < 0.05) in all groups tested, but the PM was similar between samples with and without peroxide at concentration 2.5mM of NAC. The pH of the EQUIDIL extender was higher and the EQUIDIL + 0.5mM and 1.0mM groups had intermediate values, while the 2.5mM NAC concentration generated lower values in the three evaluated periods (P < 0.05). There was no significant variation of pH between 0 and 24 h (P=0.7075) and between 0 and 48 h (P=0.4617) in all groups tested. The concentrations of NAC tested did not improve motility, plasma membrane integrity, mitochondrial activity and response to HOST equine spermatozoa cooled to 5°C and stored for 48 h. After TTR, the concentrations of NAC tested did not prevent the decrease of motility and sperm vigor in the presence of peroxide.
Kerksick, Chad M. Willoughby Darryn Scott. "Effects of prophylactic supplementation of N-acetyl-cysteine and epigallocatechin gallate on markers of oxidative stress, inflammation and apoptosis after eccentric contraction-induced injury in untrained males." Waco, Tex. : Baylor University, 2006. http://hdl.handle.net/2104/4881.
Повний текст джерелаNocentini, Benedetta. "Indagine su reazioni di sulfa-Michael di interesse in campo cosmetologico e sul trattamento ricostruttore del capello." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/15849/.
Повний текст джерелаBarbanera, Pedro Octavio. "Estudo do efeito do N-acetil-cisteína através do metabolismo energético, complexos respiratórios e estresse oxidativo no tecido hepático de ratos submetidos ao glutamato monossódico." Botucatu, 2018. http://hdl.handle.net/11449/158323.
Повний текст джерелаResumo: A obesidade é considerada um dos maiores problemas de saúde pública em muitos países, uma vez que está associada queda da qualidade de vida. Embora existam vários fatores que corroboram com o desenvolvimento para tal fato, os hábitos alimentares seja o fator relevante. Os transtornos metabólicos podem resultar em alterações na funcionalidade do fígado, podendo desenvolver Doença Hepática Gordurosa Não Alcoólica (DHGNA). Como o número de obesos e as co-morbidades associadas ao sobrepeso vêm aumentando abruptamente nas últimas décadas, vários modelos de obesidade experimental têm sido propostos para investigar os distúrbios metabólicos envolvendo suas causas e consequências. O glutamato monossódico é amplamente utilizado na culinária e também por indústrias alimentícias, contudo atua no sistema nervoso central e promove a degeneração de áreas importantes do hipotálamo que leva a distúrbios da saciedade e, consequentemente acúmulo excessivo de gordura abdominal. Com a finalidade de estudar substâncias que apresentem potencial atividade terapêutica no controle dos distúrbios metabólicos, o N-acetil-cisteína possui propriedades antioxidantes e exerce hepatoproteção. Desta forma, o objetivo do presente estudo foi evidenciar a indução da obesidade pelo glutamato monossódico e determinar o efeito do N-acetil-cisteína sobre os parâmetros calorimétricos, metabolismo energético, atividade dos complexos respiratórios e estresse oxidativo no tecido hepático. Foram utilizados 32 ratos Wins... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Obesity is considered one of the greatest public health problems in many countries, since it is associated with a drop in quality of life. Although there are several factors corroborating with the development for this fact, eating habits are the relevant factor. Metabolic disorders can result in changes in liver function, and can develop Non-Alcoholic Fatty Liver Disease (NAFLD). As the number of obese and co-morbidities associated with overweight have increased steeply in recent decades, several models of experimental obesity have been proposed to investigate metabolic disorders involving their causes and consequences. Monosodium glutamate is widely used in cooking and also in food industries, but it acts on the central nervous system and promotes the degeneration of important areas of the hypothalamus which leads to satiety disorders and consequently excessive accumulation of abdominal fat. In order to study substances that present potential therapeutic activity in the control of metabolic disorders, N-acetyl-cysteine has antioxidant properties and exerts hepatoprotection. Thus, the objective of the present study was to evidence the induction of obesity by monosodium glutamate and to determine the effect of Nacetyl-cysteine on calorimetric parameters, energy metabolism, respiratory complex activity and oxidative stress in hepatic tissue. Thirty-two Winstar male mice were used at 21 days of age. Initially the animals were distributed in two experimental groups (n = 16). Grou... (Complete abstract click electronic access below)
Doutor
Ibrahim, Marwa Awad Abdel Hamid [Verfasser]. "Immunohistochemical studies of the influence of alkylating substances on the wound healing process and the possible protective role of N-Acetyl Cysteine and Alpha-Linolenic Acid / Marwa Awad Abdel Hamid Ibrahim." Köln : Deutsche Zentralbibliothek für Medizin, 2012. http://d-nb.info/1030211507/34.
Повний текст джерелаMuranaka, Lígia Segatto. "Mecanismos envolvidos com a sobrevivência de Xylella fastidiosa em condições de estresse e efeito de N-Acetil-L-Cisteína em seu biofilme." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316427.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Xylella fastidiosa é uma bactéria Gram-negativa que causa várias doenças em diferentes espécies de plantas, como a clorose variegada dos citros (CVC) em Citrus sinensis, cujos danos econômicos são da ordem de milhões de dólares anuais. O desenvolvimento dos sintomas tem sido associado ao bloqueio dos vasos do xilema causado pela formação de um biofilme pela bactéria. Recentemente foi verificado que o biofilme de X. fastidiosa é mais resistente a compostos antimicrobianos que a forma planctônica. Essa resistência tem se mostrado um fenômeno complexo que não pode ser explicado por um único mecanismo, e sim por multifatores. Nesse sentido, a proposta desse trabalho foi identificar, por microarranjos de DNA, genes possivelmente associados à adaptação do biofilme na presença de compostos antimicrobianos, em concentrações letais para células em crescimento planctônico, mas que não inibiram o crescimento do biofilme. Foram encontrados 223 (7,87%) CDS induzidas na presença de cobre e 150 (5,29%) reprimidas. Com tetraciclina foram 450 (15,89%) induzidas e 449 (15,85%) reprimidas. Muitas sequências codificadoras envolvidas com funções da síntese proteica, metabolismo energético, divisão celular e movimentação foram moduladas negativamente em ambas as situações, sugerindo que tais modificações contribuiriam para um provável estado de resistência. Com a adição da dose subinibitória de cobre também foi observada a indução de genes relacionados à adesão e produção de toxinas, que estão envolvidos com a virulência da bactéria em planta, o que sugere esta seria aumentada possivelmente induzindo maior severidade de sintomas. Já com tetraciclina o oposto foi observado, repressão de genes relacionados à formação do biofilme e produção de toxinas. No entanto, identificamos a indução de um possível mecanismo de resposta SOS, pela qual genes relacionados ao sistema toxina-antitoxina seriam superexpressos. Esse sistema provavelmente está envolvido com a morte celular programada e formação de células persistentes. Assim é possível concluir que doses subinibitórias de compostos antimicrobianos poderiam ao invés de matar, induzir a virulência da bactéria, como ocorrido com o cobre, ou a formação de células persistentes, como observado com a tetraciclina. Outra abordagem desse trabalho foi à realização de experimentos in vitro e in vivo com um análogo de cisteína, o N-acetyl-L-cysteina (NAC), que já vem sendo utilizado na medicina para desestruturação de biofilmes de bactériaspatógenas de humanos. Resultados da quantificação da massa celular, número de célulasviáveis e exopolissacarídeos totais revelaram que todas as doses de NAC (1, 2 e 6 mg/mL) testadas nos experimentos in vitro diminuiram a formação do biofilme e inibiram o crescimento de X. fastidiosa, o que indica um possível efeito tóxico dessa substância. Nos experimentos in vivo foi observada uma grande redução dos sintomas de CVC em plantas de C. sinensis infectadas e tratadas com diferentes doses de NAC. Esses estudos abrem uma real perspectiva ao uso dessa substância para o manejo da CVC.
Abstract: Xylella fastidiosa is a Gram-negative bacterium that causes several diseases in different plant species, including citrus variegated chlorosis (CVC) in sweet orange (Citrus sinensis L. Osbeck), whose economic damage is of millions of dollars annually. The symptoms development has been associated with the blockage of xylem vessels caused by bacterial biofilm formation. Recently it was found that the biofilm of X. fastidiosa is more resistant to antimicrobial compounds than the planktonic cells. This resistance has been considered as a complex phenomenon that cannot be explained by a single mechanism, but by multi-factors. Accordingly, the intent of this study was to identify through DNA microarray technology, genes possibly involved in adaptation of biofilm cells to the presence of antimicrobial compounds in concentrations that are lethal to cells in planktonic growth, but do not inhibit the cell growth in biofilm. We found 223 (7.87%) genes induced in presence of copper and 150 (5.29%) genes repressed. For tetracycline, there were 450 (15.89%) induced genes and 449 (15.85%) repressed ones. Many genes encoding proteins related to protein synthesis, energy metabolism and cell division were negatively modulated in both, copper and tetracycline treatments, suggesting that these changes could contribute to a state of resistance. When a subinhibitory dose of copper was applied we could also observe the induction of genes related to adhesion and thus biofilm formation and toxin production, suggesting that the bacterial virulence should be increased. The opposite was found for tetracycline. However, we observed the induction of a possible SOS response mechanism in which genes related to a toxin-antitoxin system was overexpressed. This system is probably involved with programmed cell death and formation of persistent cells. We then concluded that subinhibitory doses of antimicrobial compounds could induce bacterial virulence as occurred for copper, or the formation of persistent cells, as observed for tetracycline rather than kill the cells. Another approach of this work was to carry out experiments in vitro and in vivo with an analogue of cysteine, N-acetyl-L-cysteine (NAC), which has been already used in medicine as a drug for disruption of biofilms formed by human pathogenic bacteria. Results of cellular mass quantification, number of viable cells and total exopolysaccharide content revealed that all doses (1.0, 2.0 and 6.0 mg / mL) of NAC tested in in vitro experiments decreased the biofilm formation and inhibited growth of X. fastidiosa, which indicated that this substance could also be toxic for the bacteria. In vivo experiments showed a strong reduction in CVC symptoms in C. sinensis plants infected and treated with different doses of NAC. These studies open a real prospect for the use of this compound in CVC management.
Mestrado
Genetica de Microorganismos
Mestre em Genética e Biologia Molecular
Möller, Marisa. "Behavioural, neurochemical, inflammatory and mitichondrial markers following social isolation rearing in rats before and after selected deug intervention / Marisa Möller." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9524.
Повний текст джерелаThesis (PhD (Pharmacology))--North-West University, Potchefstroom Campus, 2013
Schroeder, Ilka Elizma. "A mechanistic study of organochlorine hepatotoxicity." Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/24882.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2011.
Pharmacology
unrestricted
McKay, Hart Andrew. "Sensory neuronal protection & improving regeneration after peripheral nerve injury." Doctoral thesis, Umeå universitet, Handkirurgi, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-52.
Повний текст джерелаSelvin, David. "Regulation of Myoplasmic Ca2+ During Fatigue in KATP Channel Deficient FDB Muscle Fibres." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/26174.
Повний текст джерелаPournara, Panagiota [Verfasser]. "Untersuchungen zur Toxikokinetik von Acrylamid und seiner Metabolite N-acetyl-S-(2-carbamoyl-ethyl)-cystein und N-acetyl-S-(2-carbamoyl-2-hydroxy-ethyl)-cystein nach oraler Aufnahme acrylamidhaltiger Nahrung / Panagiota Pournara." Köln : Deutsche Zentralbibliothek für Medizin, 2010. http://d-nb.info/1005360065/34.
Повний текст джерелаLiu, Chen-Wang, and 劉振旺. "Protective Effects of Garlic Water-soluble Compound N-Acetyl-Cysteine on Leukemia Mice." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/30537179967780275759.
Повний текст джерела國立屏東科技大學
生物科技系所
104
N-Acetyl-cysteine (NAC) is an acetylated variant of the amino acid L-cysteine, is an effective antioxidant which can protect cells against oxidative stress. However, the possible protective roles of N-Acetyl-cysteine in WEHI-3-induced leukemia mice has never investigated. The protective activity of NAC on WEHI-3-induced leukemia mice was examined. Twelve BALB/c mice weighing 20g were randomly divided into three groups as follows: the first group was injected PBS and served as the control group; the second group was injected WEHI-3 leukemia cells; the third group was given NAC at a dose of 50mg/kg and then injected WEHI-3 leukemia cells. The experiment lasted a month, WEHI-3 leukemia cells injected only once at the initial. We examined the effects of NAC on the gross examination of organs, weight of mice liver and spleen. The capacity of antioxidants in vivo is determined by assessment of superoxide dismutase (SOD) activity assay and total antioxidant capacity (TAC) colorimetric assay in liver, spleen and serum. We use the Western blot method to recognize NAC inhibition p53 / p38 / caspase-3 path activaty effect in the liver of leukemia mice.Our results indicated NAC revealed the moderate antioxidant activity and protect effect against leukemia in mice.
Lai, Yung-Chieh, and 雷詠捷. "Synthesis and preparation the N-Acetyl-L-cysteine based nanogel as the drug carrier." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/ny4343.
Повний текст джерела國立東華大學
生命科學系
102
N-Acetyl-L-cysteine (NAC), a scavenger for active oxygen,exhibits anti-inflammatory effect .It contains multiple disulfide bonds synthesized to degrade after delivery of drug into the cell.It can also simultaneously act as glutathione (GSH) precursor. New carriers for triggered intracellular were investigated due to in cellular defense anti oxidative damage. 1,6-Diaminohexane was used for the synthesis of organic compounds, because the polymer compound aggregates. The purpose of this study is to design the drug delivery system by using N-Acetyl-L-cysteine(NAC)and apply the devised to control the release of drugs with DTT added to break the disulfide bonds. In this experiment, physicochemical charac- teristics of N-Acetyl-L-cysteine (NAC) ,1,6-Diaminohexane and DMSO solvent mixture were analyzed by gel electrophoresis, FT-IR,NMR,mass spectrometry(MS) to identify its structure and molecnlar weignt. Particle size and ú-potential measurements were performed. 1-(3-Dimethylamin- opropyl)-3-ethylcarbodiimide (EDC) activator and N-hydroxysulfosuccinimide sodium salt (NHS) auxiliary agents activated COOH group as a reaction. The Estradiol embedded was confirmed by a dialysis technique. Adding DTT to the synthetic polymers, was to investigate the release of the drug in the cytoplasm.
Hung-Hsuan and 鄭弘萱. "Effect of N-acetyl cysteine on curcumin inhibiting telomerase activity in A549 lung carcinoma cells." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/27938455440244923109.
Повний текст джерела中山醫學大學
醫學分子毒理學研究所
97
Telomerase is a special ribonucleoprotein for synthesizing the telomeric DNA at the ends of chromosomes. Telomerase activity is suppressed in normal human somatic tissues, but it is activated in 85~90% tumor tissues. So inhibiting telomerase activity is a useful therapeutic strategy. Curcumin, the yellow pigment in turmeric, is known to inhibit proliferation of cancer cells by arresting them at various phases of the cell cycle and to induce apoptosis in tumor cells. This study mainly explored that curcumin suppress telomerase activity throuh ROS production in A549 cells. First, curcumin inhibited A549 telomerase activity by telomerase repeat amplification protocol. Using Western blot and RT-PCR, curcumin reduced h.TERT expression in a dose dependent manner. The reporter assay show that curcumin suppress the h.TERT promoter expression through Sp1. DNA affinity precipitation assay was used to confirm that curcumin inhibited Sp1 binding on the h.TERT promoter. In addition, we analyzed that curcumin could induce calcium ion production by Flow cytometry in A549 cells. A549 cells were treated with calcium ion inhibitor of BAPTA-AM and Nifedipine, which did not recover the h.TERT supperession by curcumin. Therefore, our data suggested that suppression of telomerase activity is independent on calcium ion production. Furthermore, N-acetyl cysteine (NAC) restored the telomerase activity suppression by curcumin. The mRNA and protein expression of h.TERT decreased by curcumin was reversed by NAC. NAC also restored the expression of Sp1 reduction and h.TERT that were downregulation by curcumin. Our results strongey suggested that curcumin induced ROS production which resulted in inhibiting Sp1 binding activity and h.TERT downregulation.
Phong, Ying-Chiann, and 馮瑩茜. "Effects of N-acetyl cysteine on Lateral Line Hair Cell of Zebrafish Larvae (Danio Rerio)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/3v3686.
Повний текст джерела國立臺灣師範大學
生命科學系
107
N-acetyl cysteine (NAC), an antioxidant used as a nutritional supplement, was used to protect tilapia from oxidative stress-induced by Microcystin and Cylindrospermopsin in the aquaculture sector. The aim of the present study was to investigate the potential toxicity and lethal dose of NAC before applying it to be an antioxidant in fish. Furthermore, we test for the protective effect of NAC against neomycin-induced ototoxicity. We investigate the embryonic toxicity of NAC on zebrafish larvae by acute or chronic exposure to NAC. Our results showed that NAC caused a lethal effect on zebrafish embryo in a dose-dependent manner. By the development assessments, survival rate, body length, and heart rate significantly decreased after the NAC treatment. By labeling hair cells with FM1-43, the hair cells number decreased in both acute and chronic NAC treatment groups. Oxidative stress of lateral line hair cell increased after acute NAC treatment. Moreover, NAC reduced the density of ionocyte on zebrafish yolk sac. Our findings demonstrate that NAC may cause toxicity to zebrafish in both acute and chronic treatment.
Huang, Szu-Ping, and 黃思萍. "An exposure biomarker of aristolochic acid-containing herbs: aristolochic acid and N-acetyl-L-cysteine adduct." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/6ewhgk.
Повний текст джерелаYing, Chang Wen, and 張文茵. "To Determine the Effects of N-Acetyl Cysteine on the Expression of Thrombomodulin in Breast Cancer." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/58226706533223122427.
Повний текст джерела國防醫學院
生物化學研究所
103
Reactive oxygen species (ROS) can cause cellular injury by inducing DNA damages which can lead to tumorgenesis. So far, the evidences show that antioxidants can neutralize ROS thereby reducing cell damages. Many studies have found that ROS can promote Epithelial-mesenchymal transition (EMT) of cancer cells, in which can promote cancer metastasis and invasiveness. In addition, the majority of antioxidants are also found to have the effects of prevention or inhibition of EMT. Lots of proteins are known to participate in the EMT process such as Thrombomomodulin (TM). Recently, the dates indicate that TM is the downstream regulatory gene of Snail, which may have a crucial role on EMT process. And, the other results show that the decline of the TM can increase EMT. Furthermore, N-acetylcysteine (NAC), as an antioxidant, has been considered to enhance the functions of the TM. The purpose of this proposal is to investigate the roles of the TM on the breast cancer cells morphology and metastasis under the NAC treatment. The preliminary results show that NAC can upregulate the mRNA and protein levels of TM and inhibit the breast cancer cell migration. NAC can also downregulate the expression of biomarkers of EMT in breast cancer cells.
Chen, Jia-Shing, and 陳嘉興. "Inhibitory Effects of N-acetyl-L-cysteine on Cell Proliferation and UV Induction of PCNA in CHO-K1 Cells." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/17276217244298459314.
Повний текст джерела國立清華大學
生命科學系
88
N-acetyl-L-cysteine (NAC), a well-known antioxidant is a scavenger of free radicals that can remove excess free radicals from cells and protect cells from damage by oxidants. In cycling CHO-K1 cells, NAC can inhibit the proliferation of cells, delay the procession of cell cycle and change the morphology of cells. When the cells are treated by NAC, the morphology of cells changes from normal spindle to abnormal round gradually. But we can''t find the morphology change on quiescent cells. When the medium containing NAC is removed, cell cycle will go ahead and the change of cell morphology will reverse. Proliferating cell nuclear antigen (PCNA), an auxiliary factor of DNA polymerase δandε, is required for eukaryotic cell DNA synthesis and excision repair. Expression of PCNA is growth regulated but can be induced by variable extracellular stimuli including exposure to UV irradiation. We have found that UV-C irradiation can induce PCNA gene expression in CHO.K1 cells, no matter recovering in serum or in serum-free medium. To exclude the effect of serum stimulation, we have treated quiescent cells with NAC before and after UV-C irradiation under serum free condition. We find that NAC can inhibit UV-C induction of PCNA mRNA and protein. Although NAC is a GSH precursor, the inhibitory effect of UV induction of PCNA seems to GSH independent. The results suggest that NAC involves in anti-proliferation of CHO.K1 cells and regulation of UV-C response of PCNA.
Chung, Li-Yu, and 鍾麗玉. "Oxidative damage caused by infection with Angiostrongylus cantonensis and the mechanism in the treatment with N-acetyl-L-cysteine." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/94745979984818638233.
Повний текст джерела高雄醫學大學
醫學研究所
99
This study included three aims. The first aim was to estimate reactive oxygen species (ROS) production, antioxidants activity, and biomarkers level of oxidative damage to lipid, protein and DNA in the cerebrospinal fluid (CSF) of C57BL/6 mice infected with Angiostrongylus cantonensis. The ROS concentration in the CSF of infected mice increased gradually, and the increase in ROS in CSF became statistical significance at day 12-30 post-infection compared to that before infection, and then ROS returned to normal level at day 45 after infection. In parallel with the increase in ROS in the CSF, infected mice showed similar of changes in superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, glutathione-s-transferase, and glutathione as that in ROS in the CSF. Those antioxidants in the CSF of infected mice were all significant higher than they were before infection. However, malondialdehyde, protein carbonyl content and 8-hydroxy-2’-deoxyguanosine, biomarkers of oxidative to lipid, protein and DNA, respectively, were also significantly higher in the CSF of infected mice during this period. These results suggest that oxidative stress occur in the cells of central nervous system of mice infected with A. cantonensis. The second aim was to know the source of ROS and the regulation factor in the CSF of infected mice. Eosinophils increased in mice after infection and E-, L-selectin, ICAM-1, and VCAM-1 promoted the adherence of eosinophils to vascular endothelial cells, then they transmigrated into the CSF of infected mice. The measurement of CSF eosinophils and brain tissue homogenates found that ROS were from CSF eosinophils. IL-5 was found that it could maintain CSF eosinophils producing ROS in incubation in vitro. The third aim was to estimate the effect of N-acetyl-L-cysteine in the treatment of infected mice and the mechanism in decrease the level of ROS in the CSF of infected mice. In the incubation of CSF eosinophils from infected mice with IL-5 and N-acetyl-L-cysteine, the extracellular ROS produced by eosinophils were scavenged and the intracellular ROS in eosinophils were inhibited by N-acetyl-L-cysteine. The increase leakage of lactic dehydrogenase of eosinophil in culture medium and lower mitochondrial membrane potential of eosinophil became significant 24 hours after incubation. Further more, significantly decrease of P-ERK, significantly higher activity of caspase-3, and lower cytochrome c levels as well as caspase-9 activity were also found in the same incubation. Thus, the ERK route and cytoplasmic caspase-3 were correlated with the apoptosis of CSF eosinophils incubation with N-acetyl-L- cysteine in IL-5 rich medium. The use of N-acetyl-L-cysteine in the treatment of A. cantonensis infection in hosts are helpful in promotion apoptosis of CSF eosinophils and reduction ROS production in CSF. Indeed, 50% of BALB/c mice survived after treatment with N-acetyl-L-cysteine for 12 days at day 12 after infection.
Liu, Tzu-An, and 劉子安. "Electrochemical Determination of N-acetyl-L-cysteine at Poly(3,4-ethylenedioxythiophene) and Ionic Liquid Composite Film Modified Screen-Printed Carbon Electrodes." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/9pfdf5.
Повний текст джерела國立暨南國際大學
應用化學系
102
In this study, we modified a thin layer of poly-3,4-ethylenedioxythiophene (PEDOT) on a bare screen-printed carbon electrode (SPCE) by electrochemical methods, and then ionic liquid ([BMIM+][Cl-]) was fabricated onto SPCE/PEDOT by spin coating. The obtained SPCE/PEDOT/[BMIM+][Cl-] was employed for the determination of N-Acetyl-L-cystenine (NAC) in pH 7.0 buffer. Cyclic voltammetric studies showed two irreversible oxidation potential of NAC at 0.17 V and 0.49 V. It was suggested that the ionic liquid layer can extract NAC and thus lowered overpotentials. The other ionic liquids with different anions (OTf-, NTf2-, BF4-, Br-, PF6-) were examined for NAC detection, and only Br- and PF6- showed the two oxidation peaks. Excellent analytical features were achieved by flow injection analysis for determination of NAC under optimized conditions, the linear calibration curve was obtained from 10 μM to 600 μM for NAC and the sensitivity was 0.0024 μA/μM. The detection limit (S/N = 3) was 0.25 μM.
BELLISARIO, VERONICA. "Long-term effects of prenatal exposure to high-fat diet in an animal model of reduced oxidative stress." Doctoral thesis, 2014. http://hdl.handle.net/2158/949357.
Повний текст джерелаLi, Cheng-Feng, and 李承峰. "Regulation of the PI3K/AKT Signaling Pathway by Advanced Glycation End-products, High Glucose and N-acetyl-L-cysteine in 3T3-L1 Preadipocytes." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/93017652716385956940.
Повний текст джерелаKashyap, Srishti. "Isolation, Structure Elucidation and Functional Characterization of a Novel Cytotoxic Secondary Metabolite Phomafuranone, 2-Hydroxy-2, 4-dimethyl-5-[-1-propen-1-yl]-3(2H)-furanone, from Phoma tropica an Endophytic Fungus Isolated from Mappia foetida." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/4135.
Повний текст джерелаKraus, Torsten. "Entgiftung lipophiler Xenobiotika - die Cystein-S-Konjugat-spezifische N-Acetyl-S-Transferase." Phd thesis, 2000. http://tuprints.ulb.tu-darmstadt.de/58/1/Kraus_Tor.pdf.
Повний текст джерелаKraus, Torsten [Verfasser]. "Entgiftung lipophiler Xenobiotika - die Cystein-S-Konjugat-spezifische N-Acetyl-S-Transferase : ein repräsentatives Enzym des Mercaptursäureweges / von Torsten Kraus." 1999. http://d-nb.info/960529950/34.
Повний текст джерелаShee, Somnath. "Manipulating Bacterial and Host Reactive Oxygen Species (ROS)- based mechanisms to potentiate killing of Mycobacterium tuberculosis (Mtb)." Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5680.
Повний текст джерелаWelcome-DBT