Дисертації з теми "CYP199A4"
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Youn, Jin Young. "Polymorphisms of FSHR, ESR and CYP19A1 and ovarian stimulation outcome." Thesis, Boston University, 2012. https://hdl.handle.net/2144/31627.
Повний текст джерелаPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
Ovarian stimulation is frequently used in many assisted reproductive technologies (ART) procedures to increase the number of dominant follicles. However, achieving the optimal response to ovarian stimulation without complications can be difficult. In our study, we predict that future ovarian stimulation procedures will be more individually catered to each patient. A study that established a predictor model for ovarian stimulation showed that genetic single nucleotide polymorphisms (SNP) are good predictive factors (Fauser et al., 2007). Thus the objective of this study was to determine if there was any association between the various parameters that measure the success of ovarian stimulation and FSHR, CYP19, ESRJ and ESR2 singe nucleotide polymorphisms (SNP). A total of two hundred and four women undergoing either in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) were genotyped to study the distribution of the different SNPs. Complete clinical information was available for a range of forty nine to seventy three women to study any association between genotype and basal FSH level (day 3), basal 17 -beta estradiol level, number of follicles, number of eggs and the level of 17-beta estradiol during hCG administration. The association was studied using the one way ANOVA test and linear regression model. There was a significant correlation between the CYP 19Al rs10046 genotypes and FSH level (p=0.004), number of follicles (p=0.03) and level of 17-beta estradiol during hCG administration (p=0.04). Also, FSHR rs2268263 genotype was significantly linked to FSH level between the G-allele group (A/G and G/G) and the homozygote A group (p=0.04). Taken all together, these results suggest that several genes-especially CYP 19AJ and FSHR rs2269263- play a significant role in determining the success of ovarian stimulation.
2031-01-01
Escobar, Gabriela Fortes. "Polimorfismos genéticos dos genes CYP19A1 e NFKB1 e o risco de melanoma cutâneo." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/116777.
Повний текст джерелаAbreu, Lauriane Giselle de. "Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/.
Повний текст джерелаBackground: Up to 60% of women with endometriosis have infertility symptoms. However, mechanisms remain unclear, mainly when there is no distortion of pelvic anatomy. The multifactorial etiology and polygenic involvement of this disease have been widely accepted. Aromatase is one of the most studied molecules and there are evidences of increased expression of its gene (CYP19A1) on eutopic and ectopic endometrium in endometriosis. This enzyme, codified by the CYP19A1 gene, converts androgens to estrogens and is normally present in granulosa cells, where it plays an essential role for the intrafollicle steroidogenic production. In vitrostudies by granulosa cells culture have demonstrated reduced aromatase activity in women with endometriosis. The scarcity of studies assessing expression of the aromatase gene (CYP19A1) on these target cells stimulated the proposal of this research. The aim of this study is to quantify aromatase gene expression, by real-time PCR, in mural lutein-granulose cells of women with endometriosis undergoing assisted reproduction techniques. Patients and Methods: a case-control study was conducted on 11 women with endometriosis and 11 with male or tubal causes of infertility submitted to ovarian hyperstimulation (HOC), with a total of 12 cycles for endometriosis and 11 for the control group. There was no difference between the groups regarding age or HOC parameters. Mural lutein-granulosacells were harvested from pre-ovulatory follicles during oocyte retrieval and properly isolated. Later, RNA extraction (clorophorm/isopropanol) and reverse transcription were performed. Real-time PCR was run to quantify RNAm products of aromatase gene normalized to those from the control gene, beta-actin (relative expression). All experiments were carried out in duplicate. Results: there was no difference between the groups regarding the gene expression of CYP19A1 (aromatase) gene on mural lutein-granulosa cells (p>0.05, Mann Whitney), even if we consider separately the different stages of endometriosis (control vs. minimal/mild vs. moderate/severe, p>0.05, Kruskall Wallis). Conclusion: These results suggest that aromatase may have a complex control of its gene expression on granulosa cells and, despite of previous evidences showing its reduced activity on these target cells in endometriosis, the gene expression seems not affected by the disease, according to this study.
Viciano, Gonzalo Ignacio. "A theoretical study on the mechanism of the oxidation of substrates by human aromatase enzyme (CYP19A1)." Doctoral thesis, Universitat Jaume I, 2016. http://hdl.handle.net/10803/392148.
Повний текст джерелаLa enzima citocromo P450 aromatasa juega un papel esencial en la biosíntesis de estrógenos, y su inhibición es un objetivo importante para el desarrollo de medicamentos para el tratamiento del cáncer de mama. El objetivo principal de la esta Tesis ha sido arrojar luz sobre el mecanismo catalítico y sobre la bioquímica de esta enzima, desde el punto de vista de la química teórica. Los resultados que se presentan en esta Tesis se han dividido en tres secciones principales: (1) Estudio de la especie reactiva de la enzima aromatasa: "Compound I"; (2) Estudio de la hidroxilación del substrato natural androstenediona, a lo largo del primer subciclo catalítico de esta enzima; y (3) Estudio de la hidroxilación del Exemestano, un inhibidor esteroideo de tercera generación de la enzima aromatasa, que se utiliza actualmente en el tratamiento del cáncer de mama hormonodependiente.
Reuter, Benoit [Verfasser], and Peter [Gutachter] Fasching. "Genetische Polymorphismen im Aromatasegen (CYP19A1) und deren Assoziation mit dem Geburtsgewicht des Kindes / Benoit Reuter ; Gutachter: Peter Fasching." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2017. http://d-nb.info/1143231996/34.
Повний текст джерелаSchmid, Heidi. "" Aromatase and the Pharmacogenomic Profile - Influence of CYP19A1 polymorphisms in the response of breast cancer patients treated with aromatase inhibitors"." Master's thesis, Instituto de Ciências Biomédicas Abel Salazar, 2010. http://hdl.handle.net/10216/57191.
Повний текст джерелаSchmid, Heidi. "" Aromatase and the Pharmacogenomic Profile - Influence of CYP19A1 polymorphisms in the response of breast cancer patients treated with aromatase inhibitors"." Dissertação, Instituto de Ciências Biomédicas Abel Salazar, 2010. http://hdl.handle.net/10216/57191.
Повний текст джерелаNunes, Marília Danyelle. "Análise do polimorfismo nos genes CYP19a e CYP19b em Oreochromis niloticus da linhagem Supreme e suas relações com caracteres reprodutivos." Universidade Federal de Pelotas, 2011. http://repositorio.ufpel.edu.br/handle/ri/2590.
Повний текст джерелаThe aromatase enzyme complex is responsible for converting androgens into estrogens in vertebrates. One of the key enzymes of this complex is the cytochrome P450arom gene expression and regulation of this enzyme is directly linked to sexual differentiation. Estrogen is essential for the development of the gonads and various physiological processes, ranging from normal growth to reproductive behavior. This study aimed to correlate the polymorphisms in the genes CYP19a CYP19b. In a first moment with three strains used in aquaculture (Chitralada, Supreme and GIFT) using thirty animals of each strain and, a second time only with the Supreme strain which has also the objective of analyzing the sex ratios found in both treatments at different temperatures of water and to establish possible relationships between them, analyzing the 122 animals treated at 25 ° C and 129 animals of treatment at 35 ° C. After extraction of DNA samples were subjected to PCR using primers designed to flank a region of interest. After confirmation of amplification samples were subjected to electrophoresis on Origins apparatus for identification of alleles. In the first study was identified that there is a polymorphism in the promoter region of CYP19b in the three strains. Were observed three alleles, ranging from 141 to 123 bp. Thus the primers for amplification of microsatellites in the regulatory region of the gene CYP19b were efficient, and therefore its use for identification of mutations in this region can be employed in the typical analysis of microsatellites. In the second study was identified that there is a polymorphism in the regulatory region of the gene 10 CYP19a, 5 alleles were observed. The proportion of females and males found in the treatment at 25 ° C was 1:2,3 1:2,88 and treatment at 35 ° C. However, it can be assumed that the polymorphism present in this portion of the regulatory region may be related to sex reversal found. Whereas the attempt to select individuals with polymorphisms in the regulatory region of the aromatase gene (and CYP19a CYP19b) is an essential study to evaluate the expression levels of both, so you can see how it is being expressed in individuals that will reverse or not , allowing for the reversal temperature with a higher proportion.
O complexo enzimático da aromatase é responsável pela conversão de andrógenos em estrógenos nos vertebrados. Uma das principais enzimas deste complexo é o citocromo P450arom e a regulação da expressão gênica desta enzima está diretamente ligada à diferenciação sexual. O estrogênio é essencial para o desenvolvimento das gônadas e diversos processos fisiológicos, que vão desde o crescimento normal até o comportamento reprodutivo. Este estudo teve como objetivo correlacionar o polimorfismo nos genes CYP19a e CYP19b. Em um primeiro momento com três linhagens utilizadas na piscicultura brasileira (Chitralada, Supreme e GIFT) utilizando trinta animais de cada linhagem e, em um segundo momento somente com a linhagem Supreme a qual tem-se o objetivo também de analisar as proporções de sexo encontrado em dois tratamentos com temperaturas diferentes da água e estabelecer possíveis relações entre eles, analisando 122 animais do tratamento a 25°C e 129 animais do tratamento a 35°C. Após a extração de DNA as amostras foram submetidas a PCR utilizando os primers desenhados para flanquear uma região de interesse. Após confirmação da amplificação as amostras foram submetidas à separação eletroforética no aparato Origins para identificação dos alelos. No primeiro estudo foi identificado que existe um polimorfismo na região promotora de CYP19b nas três linhagens. Foram observamos três alelos, variando entre 141 e 123 pb. Assim os primers para a amplificação de microssatélite na região regulatória do gene CYP19b foram 8 eficientes e, portanto, seu uso para identificação de mutações nesta região pode ser empregada na análise típica de microssatélite. No segundo trabalho foi identificado que há um polimorfismo na região regulatória do gene CYP19a, onde foram observados 5 padrões de bandas (supostamente alelos). A proporção de fêmeas e machos encontrada no tratamento a 25°C foi de 1:2,3 e de 1:2,88 no tratamento a 35°C. Contudo, supõe-se que o polimorfismo presente nesta porção da região regulatória pode estar relacionado à reversão sexual encontrada. Considerando a tentativa de selecionar indivíduos que apresentem polimorfismo na região regulatória dos genes da aromatase (CYP19a e CYP19b), é indispensável um estudo para avaliar os níveis de expressão de ambos, para que se possa observar como está sendo expressado em indivíduos que revertem ou não, possibilitando assim a reversão por temperatura com uma proporção mais elevada.
Thaler, Kerstin [Verfasser], and Peter Andreas [Gutachter] Fasching. "Bedeutung von zwei Single Nucleotid Polymorphismen im Aromatase-Gen (CYP19A1) für die Entstehung einer Mammakarzinomerkrankung - Eine Fall-Kontroll-Studie / Kerstin Thaler ; Gutachter: Peter Andreas Fasching." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2017. http://d-nb.info/113917858X/34.
Повний текст джерелаBouchard, Marie-France. "Fonctions aberrantes des facteurs de transcription GATA chez l'humain : régulation de l'expression ectopique du gène CYP19A1 par GATA3/4 dans les cellules de cancer du sein et effet des mutations ponctuelles de GATA4 sur la régulation de ses gènes cibles gonadiques." Thesis, Université Laval, 2009. http://www.theses.ulaval.ca/2009/26555/26555.pdf.
Повний текст джерелаBouchard, Marie France. "Fonctions aberrantes des facteurs de transcription GATA chez l'humain : régulation de l'expression ectopique du gène CYP19A1 par GATA 3/4 dans les cellules de cancer du sein et effet des mutations ponctuelle de GATA4 sur la régulation de ses gènes cibles gonadiques." Doctoral thesis, Université Laval, 2009. http://hdl.handle.net/20.500.11794/21206.
Повний текст джерелаChao, Rebecca. "Utilising CYP199A4 from Rhodopseudomonas palustris HaA2 for biocatalysis and mechanistic studies." Thesis, 2016. http://hdl.handle.net/2440/102745.
Повний текст джерелаThesis (M.Phil.) -- University of Adelaide, School of Physical Sciences, 2016.
Gonçalves, Tiago Alexandre Martins. "Polimorfismos dos genes CYP19A1, GSTM1 e GSTT1 na infertilidade feminina." Master's thesis, 2016. http://hdl.handle.net/10400.6/6358.
Повний текст джерелаThis dissertation is part of a curricular unit named Internship of Integrated Master of Pharmaceutical Sciences, in which I performed to internships on hospital and community pharmacy. We can also find a research work referent to female infertility. The first chapter of this document, named “Polymorphisms of CYP19A1, GSTM1 and GSTT1 genes on female infertility” refers to the laboratory component, held at the Health Sciences Investigation Center of University of Beira Interior. Infertility is a clinical condition with multifactorial origin, and it should be interpreted as a couple’s disease, where genetic factors have huge impact. This way, we studied the influence of polymorphisms of CYP19A1 (substitution of a tryptophan by and arginine on codon 39), GSTM1 (deletion) and GSTT1 (deletion) genes on the development of female infertility and its impact on the response of fertility treatments. We also studied the association between genotypes and the cause of infertility potentially involved. The second chapter of this dissertation is a critical analysis of the internship held in hospital pharmacy. It took place on Unidade Local de Saúde de Castelo Branco, in a total of three hundred and twenty hours. On this section, we may find in a detailed way, pharmaceutical operations on hospital pharmacy and all legal aspects related to it. The last component of this report, refers to the internship on community pharmacy, which was held on Ferrer pharmacy, in Castelo Branco, in a total of four hundred and eighty hours. On this chapter, we may find an holistic presentation of pharmaceutical reality on this field, as well as all ethical, legal and financial matters related to it.
Tong, Sok-Keng, and 湯淑敬. "The Study of Sex Differentiation and Cyp19a1 Genes in Zebrafish, Danio Rerio." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/26679377933307375664.
Повний текст джерела國立陽明大學
生化暨分子生物研究所
98
Zebrafish is a popular model for genetic and developmental studies, yet very little is known about the mechanism of zebrafish sex determination due to the lack of discernible sex chromosomes and the difficulty of distinguishing the sex of juvenile fish. To alleviate these problems, we generated fish with predictable sex trait by methyltestosterone treatment followed by natural mating. Histology and gene expressions analysis in these juvenile fish with known gender was characterized. Our genetic selection scheme matches the prediction that female-dominant genetic factors are required to determine zebrafish sex. We also found the first sign of zebrafish sex differentiation to be ovarian gonocyte proliferation and differentiation at 10-12 days post-fertilization (dpf). Sex dimorphic expression of cyp19a1a and sox9a, the female and male somatic cell markers, respectively, was also observed at three weeks of age before the appearance of testicular morphology. Besides, we found cyp19a1b was expressed in radial glial cells just peripheral to the brain ventricles in ventral telencephalon, preoptic area, and hypothalamus. Estrogens could upregulate the expression of cyp19a1b in larval brain. We have generated transgenic zebrafish lines expressing GFP driven by cyp19a1b promoter, which exhibited fluorescence signals in adult radial glial cells and in estrogen induced fish larvae. This line provides a tool to further characterize radial glial progenitor cells as well as evaluation of xenoestrogen effects.
Sahmi, Fatiha. "La régulation du gène CYP19A1 dans les cellules de granulosa bovine in vitro." Thèse, 2013. http://hdl.handle.net/1866/11390.
Повний текст джерелаOestradiol plays an important role in reproduction in general, particularly during follicular growth. Production of estradiol requires the expression of CYP19A1 following stimulation of granulosa cells by follicle-stimulating hormone (FSH) and insulin like growth factor-1 (IGF-1). In cows, there are six promoters (1.1, 1.2, 1.3, 1.4 and 1.5 and 2) that direct transcription of CYP19A1, and promoter 2 (P2) is the major promoter used in granulosa cells. However the effect of FSH and IGF-1 on the activation of these promoters of aromatase remains unclear. Further, the CYP19A1 gene has a very short half-life and a long 3' non-translated region (3'UTR) that suggests post-transcriptional as well as transcriptional regulation. The aim of my PhD project is to study the regulation of the CYP19A1 gene in the cow. This summary is divided into two parts, the transcriptional regulation involving the promoter region and the post-transcriptional regulation involving the 3'UTR. The first part of my project was to study the transcriptional regulation of CYP19A1 gene; we measured the expression of the different promoters in luteinized or nonluteinized bovine granulosa cells following stimulation of cells with FSH or IGF-1. The results of RT-PCR showed that FSH and IGF-1 increases mRNA levels from both promoters 2 and 1.1 in non luteinized granulosa cells. Subsequent experiments showed that FSH stimulates the promoter 2 via the PKA pathway and IGF-1 stimulated promoter 2 via the PKC pathway. FSH and IGF-1 stimulate the expression of 1.1 via the PI3K pathway. In subsequent studies in luteinized cells with luciferase reporter genes driven by the specific CYP19A1 promoters, FSH stimulated promoter 1.1 in a dose dependent manner but that promoter 2 was weakly activated and not responsive to FSH. We then compared the activity of human, rat, goat and bovine promoters in luteinised bovine granulosa cells. The most significant result is that the bovine (and caprine) P2 depends on several transcription factors (NR5A2, FOXL2) whereas the human and rat promoters largely depend on cAMP. In fact, these data demonstrate a reasonably robust expression of the bovine P2 when treated with forskolin, NR5A2 and FOXL2. FOXL2 appears to be a determinant of promoter activity in ruminants. The second part of my project was to study the post-transcriptional regulation of the CYP19A1 gene. The objective was to identify the elements required for the regulation of the half-life of CYP19A1 mRNA. To do so, we generated and inserted different fragments of the 3'UTR region of CYP19A1 mRNA in the pGL3promoter vector downstream of the luciferase gene, which was then transfected into luteinized granulosa cells to assess the impact of the 3'UTR sequence on the expression of luciferase reporter gene. We identified a sequence of 200 bp between 926 and 1134 bp of the 3'UTR region of CYP19A1 mRNA that significantly reduced luciferase activity. The same fragments were inserted into the pGEMTeasy vector for in vitro transcription and the generation of mRNA for UV crosslinking with protein extracts to demonstrate the presence of mRNA/protein complexes. We detected protein complexes of 66 and 80KDA that specifically bound to the 200 pb probe. This protein is expressed in granulosa cells but not in other tissues such as the liver and heart. The use of reporter gene attracted the interest of a company producing equine chorionic gonadotropin (eCG), and an interest was expessed in developing this system to measure the FSH-like bioactivity in eCG, and therefore have a more effective commercial product. In this study, we developed a FSH bioassay system based on the transfection of cells with an the FSH receptor and a colorimetric reporter gene to estimate the activity of FSH in the serum of the mare ; these results may be applicable at the farm / industry.
Chang, Ning, and 張寧. "Target-Oriented TCM identification from Bai Hua She She Cao: Inspiration of CYP19A1 inhibitor in Gastric Cancer Therapy." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/hv2wv9.
Повний текст джерелаGuyón, Noelia Fernanda. "Expresión de Aromatasa Cerebral y Gonadal y cambios histológicos gonadales como biomarcadores de exposición a contaminantes ambientales." Doctoral thesis, 2019. http://hdl.handle.net/11086/11607.
Повний текст джерелаEl objetivo general de la presente Tesis Doctoral fue realizar un análisis integral, que contemple el efecto de la exposición a contaminantes ambientales sobre biomarcadores de diferentes niveles de organización (moleculares, histológicos y somáticos) en peces. Para ello se evaluó la respuesta de los genes cyp19a1 (aromatasa), la histología de las gónadas y los índices somáticos en machos de la especie íctica autóctona Jenynsia multidentata tanto en condiciones de laboratorio como a campo. En bioensayos se analizaron los cambios en los biomarcadores frente a la exposición a 17β‐estradiol, 17α‐etinilestradiol, 4n‐nonilfenol y 17α‐metiltestosterona; mientras que a campo se realizó una caracterización de la calidad del agua y de los niveles de expresión de los genes cyp19a1, y se compararon las respuestas de los biomarcadores entre dos sitios con diferente calidad de agua de la cuenca del Río Suquía. Los resultados obtenidos en esta tesis demuestran que la aromatasa cerebral responde como un biomarcador sensible frente a la exposición a compuestos que presentan actividad estrogénica en condiciones de laboratorio, respondiendo incluso ante la concentración más baja de estos compuestos.El análisis histológico de los testículos reveló un gran impacto de la exposición a los diferentes químicos sobre este órgano. A diferencia de lo observado a nivel molecular y tisular, los índices somáticos (Factor de Condición, Índice Hepatosomático e Índice Gonadosomático) no mostraron marcados cambios frente a la exposición a compuestos estrogénicos. A campo se observaron fluctuaciones de la expresión de la aromatasa cerebral a lo largo del año, siendo máxima durante la estación reproductiva en ambos sitios de muestreo, sugiriendo su implicancia en el control del ciclo reproductivo. Se observó un desfasaje en el incremento de la expresión del gen entre los sitios, registrándose el aumento con un mes de retraso en el sitio contaminado respecto al de referencia,lo cual podría estar relacionado con desfasajes en el comienzo del ciclo reproductivo. Los índices somáticos revelaron efectos adversos debidos a la exposición a poluentes ambientales y la histología de los testículos se vio severamente afectada en el sitio contaminado,evidenciando lesiones que incluyeron la desorganización del tejido testicular, alteraciones severas de las células germinales, presencia de células vacuolares, desincronización del proceso de espermatogénesis y necrosis. Las concentraciones de los compuestos utilizados para los ensayos de laboratorio se encuentran dentro del rango detectado en aguas superficiales de Argentina,demostrando que los peces están expuestos a estos niveles de contaminantes en el ambiente. Los cambios deletéreos observados en J. multidentata frente a la exposición crónica a contaminantes ambientales (tanto en condiciones de laboratorio como a campo), podrían afectar su capacidad reproductiva y representar una amenaza para la especie.
Godinho, João Paulo Baptista. "Expressão de genes de baixa penetrância no prognóstico do cancro da mama." Master's thesis, 2014. http://hdl.handle.net/10400.6/4995.
Повний текст джерелаBreast cancer is a pathology with great impact in female population, because it is associated with considerable incidence, prevalence, morbidity and mortality. Genetic heritage represents an important role in this nosological entity. However, mutations that confer high risk of developing this disease only explain a small proportion of the cases. Low penetrance genes are an important susceptibility factor and are being the target of several studies in populations. Research accomplished by our group within the population that Centro Hospitalar Cova da Beira covers, pointed to an association between low penetrance genes polymorphisms and the risk of breast cancer. These polymorphisms are “CYP19A1 Trp39Arg (T/C)”, “GSTT1 null” and “GSTM1 null”. We conducted a case-control study in a sample of 37 women belonging to the population covered by Centro Hospitalar Cova da Beira. Our main goal was to determine if the 3 polymorphisms (“CYP19A1 Trp39Arg (T/C)”, “GSTT1 null” and “GSTM1 null”) have different expression levels, between women diagnosed with breast cancer, but with distinct prognoses. Our focus was based in two main parameters, “Clinical staging” and “Regional lymph node involvement”. In this research work we used paraffin embedded tissues of breast. Expression quantification was accomplished by Real Time PCR. Statistical analysis was performed recurring to the Pearson’s chi-squared test and nonparametric tests. To estimate expression levels differences we used the CT comparative method, 2-??CT. GSTT1 and GSTM1 results were influenced by small RNA quantities extracted from the paraffin embedded tissues. Nevertheless, despite difficult RNA extraction, we obtained a high efficacy in the laboratory methodologies depending on this procedure, about 81.1%. Regarding CYP19A1 Trp39Arg expression, 36.7% of the studied cases were detected. After result validation we noticed that this expression was different between two groups of distinct clinical staging. “Stage III” expresses 4.33 more CYP19A1 Trp39Arg than “Stages I and II”, being aware that the presence or absence of the Estrogen receptor has to be considered to interpret the results correctly. We speculate that this aromatase polymorphism expression may have some kind of impact in the determination of breast cancer prognoses in the studied population.