Дисертації з теми "CULTURE BASED"
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1
Krings-Ernst, Dennis. "Developing the Culture-Based View of the Firm A culture-based Approach to Competitive Advantage /." St. Gallen, 2007. http://www.biblio.unisg.ch/org/biblio/edoc.nsf/wwwDisplayIdentifier/03605573002/$FILE/03605573002.pdf.
Повний текст джерела
2
Ferreira, Raquel. "Culture and E-Commerce: Culture Based Preferences for Interface Information Design." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/34417.
Повний текст джерелаАнотація:
Global companies face the challenge of offering their products or services to a wider audience. While the Internet has made it easier to distribute information globally, information design for different cultures is still very difficult. Cultural models can be used to identify differences between cultures that may have an effect on how people make decisions. Geert Hofstede (1980, 1997) has defined a cultural model with five dimensions. One of these dimensions, individualism vs. collectivism has been shown to affect the type of information people prefer when viewing printed advertisements.
A study was conducted to determine if the individualism vs. collectivism dimension affects the type of information Anglo-American and Hispanic-American people prefer for purchasing tasks in a computer-based environment. The findings of this study suggest that there is no difference between the cultures on preference for information. Nonetheless, there are cultural aspects that have to be considered when designing interfaces for a Hispanic audience as opposed to an Anglo-American audience. Based in these cultural aspects, design guidelines were developed. These aspects are the basis of the design guidelines provided in this paper.Master of Science
3
Materassi, Costanza <1990>. "Produzioni culture-based di Murano e Burano: l’engagement culturale delle imprese." Master's Degree Thesis, Università Ca' Foscari Venezia, 2019. http://hdl.handle.net/10579/14595.
Повний текст джерелаАнотація:
Nell'ambito delle industrie culturali e creative, si è approfondito il discorso del vetro artistico di Murano come modello di imprese caratterizzate da business model culture-based. Esse sono state analizzate insieme alle imprese di merletto ad ago ancora presenti sull'Isola di Burano, si è instaurato così un confronto tra le situazioni presenti e passate. Obiettivo della ricerca è indagare il coinvolgimento culturale di imprese business model culture-based, lo sviluppo di partnership tra aziende e organizzazioni culturali, enti ed istituzioni del territorio veneziano. Analizzando l’evoluzione delle produzioni di Murano e di Burano dalle origini ad oggi, si osserva l’ecologia di un sistema basato sulla relazione tra aziende, comunità e territorio. Si rintracciano gli strumenti teorici per comprendere il rapporto che intercorre tra le produzioni culture-based e la cultura; alla luce di queste considerazioni si prendono in esame le manifestazioni internazionali legate al mondo del vetro d'arte e del merletto come esempi di engagement culturale. La ricerca si concentra, nella sua fase operativa, sulle forme locali di engagement culturale: si presentano i risultati dell'intervista portata avanti ad alcune imprese significative per il loro approccio alle azioni culturali.
4
Tok, Nafiz. "Culture, identity and politics : an identity-based approach to culture-related issues." Thesis, University of Exeter, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365174.
Повний текст джерела
5
Chen, Michael C. W. "Hydrogel-based microfluidic system for cell culture." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/7209.
Повний текст джерелаАнотація:
Traditionally, cell culture has been done in culture flasks or well plates where the volumes and length scales involved in the culture environment are many orders of magnitude larger than the size scale of individual cells. To better tailor medical care to an individual patient, it may be necessary to carry out genetic, physiological, and biochemical analyses on very small cell samples and to have an in vitro cell culture environment that more closely approximates the in vivo conditions.
A microfluidic device that integrates both cell handling and long-term 3-D cell culture techniques is presented. The designed microdevice traps cells with alginate, an ionically cross-linking hydrogel, which mimics the extra cellular matrix within our body. To encapsulate the cells, a solution of calcium ions is introduced in parallel with the alginate precursor cell suspension. Alginate hydrogel forms at the interface and as the region of gel grows it traps cells inside. This is a reversible process; the gel matrix can be dissolved and the cells can be released by the addition of ethylene-diaminetetraacetic acid (EDTA), a calcium chelator.
To show that the microfluidic device is reliable for long term mammalian cell culture, hepatocytes and breast tumor cells were cultured within the alginate gel layer inside the microfluidic device for more than two weeks. Hepatocytes were able to form three-dimensional aggregates within the microfluidic hydrogel environment. We further demonstrate the possibility of performing anticancer agent screening within this device. Breast tumor cells seeded in the microchannel were treated with doxorubicin, a common chemotherapy drug. Compared to controls, the doxorubicin inhibited cell proliferation.
In future, this system will have applications in cell-based testing and in studies involving small cell populations, such as cancer cells obtained from needle biopsies of tumors.
6
Wiratama, Basiswanto. "Performance-Based Budgeting and the National Culture of Indonesia: How Good is the Fit?" Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/24673.
Повний текст джерелаАнотація:
PBB represents an important dimension of public administration. A significant problem arises from tensions between the values of PBB, which originated from developed countries, and the cultural context of developing countries. If PBB cannot be adapted to the societal culture, it is likely to be resisted, and implementation will likely be ineffective. Nevertheless, PBB may transform budgeting culture because there is a bi-directional relationship between culture and PBB.
Using Hofstede’s theory of culture as its analytical starting point, this thesis advances the understanding of not only budgeters’ preferences in implementing PBB as a manifestation of culture, but also culture’s relationship to PBB as the ‘best practice’ recommended by intergovernmental organisations (IGOs). The thesis also examines how Indonesia’s Ministry of Finance, as the designer of PBB, adapts PBB to suit the Indonesian cultural context.
The thesis has two main findings. First, the budgeters value strong hierarchies and generally prefer centralisation over the decentralisation recommended by IGOs’. Secondly, the budgeters generally embed ‘collectivism’ and group-based reward and sanction systems rather than individually based systems. However, with regard to Hofstede’s other cultural indicators—status, symbolism, security and stability in budgeters’ orientation to performance management and flexibility—the data were nuanced. These values did not strongly influence the budgeters’ preferences for performance measurement and flexibility.
In relation to adaptation of PBB principles, PBB designers did not generally adapt their practices and procedures to suit Indonesia’s dominant societal culture. The main implication for the Indonesian government is that its PBB approach should favour top-down and group-based rewards and sanctions, if PBB is to gain greater acceptance among implementers.
7
Crawford, Dana Elaine. "Therapists’ Awareness, Identification, and Management of Culture-based Countertransference." Miami University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=miami1334711065.
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8
Meng, Nan. "Chinese Culture themes and Cultural Development: from a Family Pedagogy to a Performance-based Pedagogy of a Foreign Language and Culture." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1345312833.
Повний текст джерела
9
Prociv, Patricia Mary, of Western Sydney Hawkesbury University, and Faculty of Social Inquiry. "Personal identity and the image-based culture of Catholicism." THESIS_FSI_XXX_Prociv_P.xml, 2000. http://handle.uws.edu.au:8081/1959.7/318.
Повний текст джерелаАнотація:
This research is documented in three volumes, and is the study of a series of three Doctoral exhibitions. The first of these, Australian moon over Cumbria and the procession of life, evolved from a series of watercolours based on the biblical figures of Eve and the serpent.The volume contains images and a critique from Australian moon over Cumbria. Also included are images that influenced the work, essays, and information on relevant minor exhibitions. The second, Sisters and spinsters, the Misses Swann of Elizabeth Farm, was designed and executed as site-specific.The Misses Swann were nine sisters, and the exhibition focused on the sisters' working lives, their contribution to their local communities, and their personalities.Needlepoint and damask table napkins were used as vehicles for the storytelling.Critical writings and extensive reference material are included. The third in the series, Constructing identity within Catholicism, was based on the hypothesis that images of the culture of Catholicism have the capacity to influence personal identity. All of the work was designed to complement the design and spiritual meaning of the chapel. Included along with the essays are supporting images and documentation.Doctor of Creative Arts
10
Doherty, Oran. "The impact of organisational culture on work-based learning." Thesis, Northumbria University, 2016. http://nrl.northumbria.ac.uk/32558/.
Повний текст джерелаАнотація:
Higher education institutes (HEIs) and external employer organisations are increasingly recognising the benefits of engaging in work-based learning (WBL) partnerships. However, significant challenges associated with this form of engagement have meant such partnerships are not as widespread as they could be. One of the major challenges identified relates to organisational culture. The purpose of this study is to consider how WBL partnerships between an Irish HEI (HEI X) and external employer organisations can be enhanced by a deeper understanding of organisational culture. An ethnographic methodology combining a number of different data-gathering methods, including observation, reviewing of documents and interviews with eight HEI X staff, eight WBL learners and five employer representatives, was adopted. The findings reveal how cultural differences in relation to assumptions, timeframes, languages, objectives and general attitudes can be a source of difficulty for the three stakeholders (learner, employer and HEI). According to the findings, cultural issues within the HEI and external employer organisation can also create significant challenges when attempts are being made to initiate or coordinate a WBL partnership. Johnson’s cultural web (1988) is used as a framework to present the findings. The study makes a valuable contribution to knowledge by recognising the requirements of all three stakeholders and discussing the usefulness of the cultural web as a framework for considering organisational culture in WBL partnerships. The study also makes a valuable contribution to practice by presenting recommendations to the HEI and external employer organisation. The recommendations for the HEI involve a change in the “way we do things around here” due to the unique characteristics of WBL programmes, which may mean adapting existing policies, procedures and systems. Recommendations for the employer organisation include providing support to the learner, understanding and respecting the HEI’s requirements, and ensuring that internal policies, procedures, practices and priorities support WBL.
11
YINDERI, Nila. "NOMAD - A luminaire design based on the nomadic culture." Thesis, KTH, Ljusdesign, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-282874.
Повний текст джерелаАнотація:
The intention of this work is to represent the nomadic culture by a luminaire. Inspired by the nomadic Mongolian ger(yurt) and based on its physical and cultural characters, the work is done by designing a portable luminaire with the concept of the solar solution. The whole project is an attempt of bringing traditional culture items to luminaire design and searching for the balance between them.
12
JING, GAOSHAN. "CULTURE-BASED MICROFLUIDIC DEVICE FOR RAPID DETECTION OF MYCOBACTERIA." University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1093031753.
Повний текст джерела
13
Mishina, Christy Lokelani. "Hawaiian Culture-Based Education| Reclamation of Native Hawaiian Education." Thesis, Prescott College, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10275900.
Повний текст джерелаАнотація:
American colonization of the Hawaiian Islands has brought about generations of Native Hawaiian learners being subjected to educational practices that are incompatible with core Indigenous beliefs. Consequently, Native Hawaiian learners have lower academic achievement than other ethnic groups in the islands. The lack of success is not confined to academics since Native Hawaiians are also underrepresented in material-economic, social-emotional, and physical wellbeing. Hawaiian culture-based education (HCBE) can be used to decolonize educational practices by increasing cultural relevancy and compatibility within schools. This study was conducted within a school founded explicitly for the education of Native Hawaiian children. The selected campus has approximately 80 teachers and 650 Native Hawaiian learners (age eleven to fifteen). The purpose of the study was to better understand implementation of the HCBE framework components and data was collected through surveys and semi-structured follow-up interviews. The findings showed that although there was a range of the extent the teachers at the school understood and implemented the various HCBE components, there was commitment to using Hawaiian language, knowledge, and practices as the content and context for student learning. The data also showed though teachers have a high level of understanding of the importance of relationship building, that building family and community relationships remains an area of challenge. Additionally, teachers pride themselves on delivering meaningful personalized learning experiences and assessments to their students, and would like their own professional development to be grounded in the same educational practices. This study provides baseline data to inform further growth.
14
Prociv, Patricia Mary. "Personal identity and the image-based culture of Catholicism." Thesis, View thesis View thesis, 2000. http://handle.uws.edu.au:8081/1959.7/318.
Повний текст джерелаАнотація:
This research is documented in three volumes, and is the study of a series of three Doctoral exhibitions. The first of these, Australian moon over Cumbria and the procession of life, evolved from a series of watercolours based on the biblical figures of Eve and the serpent.The volume contains images and a critique from Australian moon over Cumbria. Also included are images that influenced the work, essays, and information on relevant minor exhibitions. The second, Sisters and spinsters, the Misses Swann of Elizabeth Farm, was designed and executed as site-specific.The Misses Swann were nine sisters, and the exhibition focused on the sisters' working lives, their contribution to their local communities, and their personalities.Needlepoint and damask table napkins were used as vehicles for the storytelling.Critical writings and extensive reference material are included. The third in the series, Constructing identity within Catholicism, was based on the hypothesis that images of the culture of Catholicism have the capacity to influence personal identity. All of the work was designed to complement the design and spiritual meaning of the chapel. Included along with the essays are supporting images and documentation.
15
Prociv, Patricia Mary. "Personal identity and the image-based culture of Catholicism." View thesis View thesis, 2000. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030520.145146/index.html.
Повний текст джерела
16
Badia, Gelabert Eulàlia. "The organizational and safety culture of the Spanish nuclear industry. A descriptive approach based on 20 years of independent safety culture assessments." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/673258.
Повний текст джерелаАнотація:
Les anàlisis posteriors a l'ocurrència dels accidents nuclears més importants (Three Mile
Island, Chernobyl i Fukushima) han posat de manifest que les causes dels incidents de sistemes
tecnològics complexos no solament estan vinculades a aspectes tècnics, sinó que factors
aparentment més imperceptibles, com el comportament humà o la cultura organitzacional,
juguen un paper important en el seu desenvolupament (Rasmussen, 1997).
En la indústria nuclear espanyola, l'estudi d'incidents com l'incendi en l'edifici de les
turbines de la central nuclear (C. N.) de Vandellòs I (1989), la corrosió dels sistemes de refrigeració en
la C.N. de Vandellòs II (2004) i l'alliberament de material radioactiu en la C.N. d'Ascó I (2008),
també ha evidenciat la influència del component humà i organitzatiu en l'operació segura
de les instal·lacions.
En aquest context, la present tesi doctoral té el propòsit investigador de tractar d'identificar
trets característics de la cultura de les organitzacions nuclears espanyoles a partir de l'anàlisi dels
resultats de les avaluacions externes de la cultura organitzativa i de seguretat realitzades al sector. El coneixement d'aquests trets culturals pot ajudar a comprendre el comportament humà en un context on la seguretat ha de ser un dels fins de l'organització.
Per a això, es proposen tres objectius específics plasmats en els tres estudis empírics que conformen la tesi:
1) Descriure la cultura organitzativa del sector nuclear espanyol i identificar la possible existència de diferents subcultures dins de la indústria (Badia, Navajas i Losilla, 2020).
2) Descriure alguns dels factors rellevants per al desenvolupament de les característiques de cultura de seguretat sobre la base de tres teories científiques sobre organitzacions complexes (Badia, Navajas i Losilla, 2021).
3) Identificar, en un estudi de cas, els factors que determinen el comportament notificador del personal en el sistema de reporti de problemes (Navajas i Badia, 2020).
Aquesta tesi aporta coneixement sobre alguns dels patrons normatius i trets culturals de la indústria nuclear espanyola que impacten en la seguretat de les instal·lacions. Aquest coneixement pot ser utilitzat pel sector per a la millora de l'acompliment i l'operació segura de les plantes.
En aquest sentit, la similitud cultural entre centrals nuclears podria ser considerada per les diferents organitzacions per a treballar col·lectivament a optimitzar el seu funcionament. Compartir actuacions en l'àmbit dels factors humans i organitzatius i buscar estratègies per a gestionar la
variabilitat cultural no sols podria ser un factor clau per a la seguretat, sinó que els permetria fer front conjuntament als desafiaments als quals s'enfronta la indústria amb l'abandó progressiu de l'energia nuclear.
Així mateix, aquesta tesi obre noves línies de recerca orientades a aprofundir en alguns de les troballes obtingudes com són la gestió de les diferències culturals entre col·lectius per part de les organitzacions o l'estudi de la influència en la seguretat de les diferents interaccions culturals.Los análisis posteriores a la ocurrencia de los accidentes nucleares más importantes (Three Mile Island, Chernobyl y Fukushima) han puesto de manifiesto que las causas de los incidentes de sistemas tecnológicos complejos no solamente están vinculadas a aspectos técnicos, sino que factores aparentemente más imperceptibles, como el comportamiento humano o la cultura organizacional, juegan un papel importante en su desarrollo (Rasmussen, 1997). En la industria nuclear española, el estudio de incidentes como el incendio en el edificio de las turbinas de la central nuclear (C. N.) de Vandellòs I (1989), la corrosión de los sistemas de refrigeración en la C.N. de Vandellòs II (2004) y la liberación de material radiactivo en la C.N. de Ascó I (2008), también ha evidenciado la influencia del componente humano y organizativo en la operación segura de las instalaciones. En este contexto, la presente tesis doctoral tiene el propósito investigador de tratar de identificar rasgos característicos de la cultura de las organizaciones nucleares españolas a partir del análisis de los resultados de las evaluaciones externas de la cultura organizativa y de seguridad realizadas al sector. El conocimiento de dichos rasgos culturales puede ayudar a comprender el comportamiento humano en un contexto donde la seguridad debe ser uno de los fines de la organización. Para ello, se proponen tres objetivos específicos plasmados en los tres estudios empíricos que conforman la tesis: 1) Describir la cultura organizativa del sector nuclear español e identificar la posible existencia de diferentes subculturas dentro de la industria (Badia, Navajas y Losilla, 2020). 2) Describir algunos de los factores relevantes para el desarrollo de las características de cultura de seguridad en base a tres teorías científicas sobre organizaciones complejas (Badia, Navajas y Losilla, 2021). 3) Identificar, en un estudio de caso, los factores que determinan el comportamiento notificador del personal en el sistema de reporte de problemas (Navajas y Badia, 2020). Esta tesis aporta conocimiento sobre algunos de los patrones normativos y rasgos culturales de la industria nuclear española que impactan en la seguridad de las instalaciones. Este conocimiento puede ser utilizado por el sector para la mejora del desempeño y la operación segura de las plantas. En este sentido, la similitud cultural entre centrales nucleares podría ser considerada por las distintas organizaciones para trabajar colectivamente en optimizar su funcionamiento. Compartir actuaciones en el ámbito de los factores humanos y organizativos y buscar estrategias para gestionar la variabilidad cultural no sólo podría ser un factor clave para la seguridad, sino que les permitiría hacer frente conjuntamente a los desafíos a los que se enfrenta la industria con el abandono progresivo de la energía nuclear. Asimismo, esta tesis abre nuevas líneas de investigación orientadas a profundizar en algunos de los hallazgos obtenidos como son la gestión de las diferencias culturales entre colectivos por parte de las organizaciones o el estudio de la influencia en la seguridad de las diferentes interacciones culturales.
Post-accident analyses of the most important nuclear accidents (Three Mile Island, Chernobyl and Fukushima) have revealed that the causes of incidents involving complex technological systems are not only linked to technical aspects, but also to seemingly more imperceptible factors, such as human behavior or organizational culture which play an important role in their development (Rasmussen, 1997). In the Spanish nuclear industry, the study of incidents such as the fire in the turbine building at Vandellòs I NPP (1989), the corrosion of the cooling systems at Vandellòs II NPP (2004) and the release of radioactive material at Ascó I NPP (2008), has also evidenced the influence of the human and organizational component in the safe operation of the facilities. In this context, the purpose of this doctoral thesis is to try to identify characteristic features of the culture of Spanish nuclear organizations based on the analysis of the results of the external evaluations of the organizational and safety culture carried out in the sector. Knowledge of these cultural traits can help to understand human behavior in a context where safety must be one of the organization's goals. To this end, three specific objectives are proposed in the three empirical studies that make up the thesis: 1) To describe the organizational culture of the Spanish nuclear industry and identify the possible existence of different subcultures within the industry (Badia, Navajas and Losilla, 2020). 2) To describe some of the relevant factors for the development of safety culture characteristics based on three theories on complex organizations (Badia, Navajas and Losilla, 2021). 3) To identify, in a case study, the factors that determine the reporting behavior of personnel in the problem reporting system (Navajas and Badia, 2020). This thesis provides knowledge on some of the normative patterns and cultural traits of the Spanish nuclear industry that impact on the safety of the facilities. This knowledge can be used by the industry for the improvement of plant performance and safe operation. In this sense, the cultural similarity between nuclear power plants could be considered by the different organizations to work collectively to optimize their operation. Sharing actions in the field of human and organizational factors and looking for strategies to manage cultural variability could not only be a be a key factor for safety, but would also allow them to jointly address the challenges they face with the progressive withdrawal of nuclear power energy. Likewise, this thesis opens new lines of research aimed at deepening some of the findings obtained, such as the management of cultural differences between groups by organizations or the study of the influence of different cultural interactions on safety.
Universitat Autònoma de Barcelona. Programa de Doctorat en Psicologia de la Salut i de l'Esport
17
Lin, Dai. "Culture teaching in ELT : a study of a culture-based course in undergraduate English programmes in China." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/50658/.
Повний текст джерелаАнотація:
This research is designed to investigate the treatment of culture in education policies, teachers’ beliefs and the current practices of culture teaching in undergraduate English programmes in China, with a particular focus on the teaching of the culture-based course A General Survey of English-speaking Countries. This qualitative research involves documentation, interview and classroom observation as the main methodological tools for data collection. The National Curriculum for English Majors is analysed to find out how culture is treated in ELT education policies. 10 teachers from 10 higher institutions in Shanghai, China were interviewed and two individual case studies were carried out using interview and classroom observation data and other supplementary data. A model of multi-layered analysis is adopted. Through the triangulation of various methods and data sources, policy-making at the macro-level is linked to teachers’ perceptions of culture-teaching, then to their classroom practices at the micro-level. In this way analysis, description and explanation of how culture is being taught in English language programmes in China are provided. The findings of the current study suggest that: 1) the national guidelines for undergraduate English programmes have a clear intention for a strong commitment to promote students’ cultural understanding and intercultural communicative skills, but the impractical curricular policies can become potential constraints to the teaching of culture. In the process of interpretation, institutions and departments are not playing a sufficient part in curriculum and syllabus design and development, and teaching in the classroom is often without clear guidelines; 2) Teachers are increasingly aware of the dynamic and variable nature of culture, which academia strongly suggest should be incorporated into its teaching. However, concerning target cultures in ELT, teachers are predisposed to British and American cultures. They have encountered a series of challenges such as an overloaded syllabus, the overwhelming task of preparation, fear of lacking overseas experience and knowledge, lack of institutional support and relevant training, students’ lack of motivation and large classes. These challenges can be attributed to two major factors: the complex nature of culture teaching and the low status of culture-based courses in language programmes; 3) Teachers’ attitudes, beliefs and their cultural experiences have impact on their pedagogical choices. There are shared patterns as well as variations in teachers’ pedagogical approaches. The actual classroom practices are mostly teacher-centred and involve merely transfer of knowledge centred on the surface level of cultural knowledge. The cultures of the UK and the US are considered to be representative of the target cultures and dominate the content in teaching. In addition, the integration of culture and language in the classroom practices manifests itself in different ways. In one approach, language instruction is frequently inserted into teaching as it is considered to be one aspect of culture teaching. In another, and more common approach when culture teaching is equated with imparting cultural knowledge, teachers often give up English as the sole medium of instruction and make use of Chinese as a support for culture teaching. Furthermore, Chinese culture is also integrated to enhance cross-cultural comparisons and make culture teaching more efficient. The research findings have a number of implications for further study, as well as tentative suggestions for curriculum design and implementation, teacher education and teaching practices. It is hoped that they will provide a new perspective on the complexity and intricacy of the matter of culture teaching.
18
Chandler, Jennifer V. "Why culture matters : an empirically-based pre-deployment training program /." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2005. http://library.nps.navy.mil/uhtbin/hyperion/05Sep%5FChandler.pdf.
Повний текст джерела
19
Menkel, F. "Enhancement of fermenter design procedures based on batch culture analysis." Thesis, Swansea University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.638179.
Повний текст джерелаАнотація:
The objective of this work was to develop an unstructured, deterministic and uniform mathematical model of cellular activity in a liquid phase batch culture, including the initial lag phase. A new mathematical model, termed the algebraic model, has been developed for predicting cellular activity of a liquid phase batch culture. In contrast to the Monod (1949), model the algebraic unstructured mathematical rate model is based on the natural order of cellular processes, namely, substrate uptake, followed by conversion into energy and new cells. The specific substrate uptake rate is modelled by a hyperbolic Michaelis-Menten type equation, as a function of the environmental substrate concentration. Conversion of carbon substrate into new cells and energy is modelled by a variable yield concept, which incorporates the requirements of cell internal maintenance. The algebraic model can also simulate the initial lag behaviour of a batch culture. In order to test the algebraic model, a Windows based computer program has been written and was used to analyse 49 batch culture experiments of Young (1991) and 22 of Patel (1991) for the aerobic culture of Saccharomyces cerevisiae on glucose as the carbon and energy limiting substrate in a chemically defined liquid medium. These batch culture experiments were simulated by only two cellular activity phases, namely, the start phase, and the main phase. An initial linear substrate uptake phase, or start phase, was observed for all the experimental data analysed, followed by exponential substrate uptake and cell growth during the main phase. The duration of the start phase was predicted by a biomass doubling concept. The start phase ended when the biomass concentration in the batch culture had doubled with reference to the initial biomass concentration after inoculation.
20
Herberg, Maria, Tüzer Kalkan, Ingmar Glauche, Austin Smith, and Ingo Roeder. "A Model-Based Analysis of Culture-Dependent Phenotypes of mESCs." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-147356.
Повний текст джерелаАнотація:
Mouse embryonic stem cells (mESCs) can be maintained in a proliferative and undifferentiated state over many passages (self-renewal) while retaining the potential to give rise to every cell type of the organism (pluripotency). Autocrine FGF4/Erk signalling has been identified as a major stimulus for fate decisions and lineage commitment in these cells. Recent findings on serum-free culture conditions with specific inhibitors (known as 2i) demonstrate that the inhibition of this pathway reduces transcription factor heterogeneity and is vital to maintain ground state pluripotency of mESCs. We suggest a novel mathematical model to explicitly integrate FGF4/Erk signalling into an interaction network of key pluripotency factors (namely Oct4, Sox2, Nanog and Rex1). The envisaged model allows to explore whether and how proposed mechanisms and feedback regulations can account for different expression patterns in mESC cultures. We demonstrate that an FGF4/Erk-mediated negative feedback is sufficient to induce molecular heterogeneity with respect to Nanog and Rex1 expression and thus critically regulates the propensity for differentiation and the loss of pluripotency. Furthermore, we compare simulation results on the transcription factor dynamics in different self-renewing states and during differentiation with experimental data on a Rex1GFPd2 reporter cell line using flow cytometry and qRT-PCR measurements. Concluding from our results we argue that interaction between FGF4/Erk signalling and Nanog expression qualifies as a key mechanism to manipulate mESC pluripotency. In particular, we infer that ground state pluripotency under 2i is achieved by shifting stable expression pattern of Nanog from a bistable into a monostable regulation impeding stochastic state transitions. Furthermore, we derive testable predictions on altering the degree of Nanog heterogeneity and on the frequency of state transitions in LIF/serum conditions to challenge our model assumptions.
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Elhaneid, Mohamed. "Toxicological assessment of graphene based nanomaterials in cell culture models." Thesis, University of Birmingham, 2019. http://etheses.bham.ac.uk//id/eprint/8881/.
Повний текст джерелаАнотація:
Graphene oxide (GO) and reduced GO (r-GO) nanomaterials exhibit great potential for several biomedical applications. Of foremost importance is to determine any potential health hazards related in their exposure. In this research, we hypothesised that the different material properties evidenced by GO and r-GO would elicit different biological responses. The first objective of this work was to synthesize Go and r-GO and characterize their physiochemical properties. The second aim was to investigate whether the two-distinct surface chemistries of GO and r-GO influenced their biological effect. The potential toxicity of these nanomaterials was investigated using the normal lung fibroplast cell line MRC-5 and cancerous epithelial lung cell line A549. The cytotoxicity of graphene derivatives was concentration-, time- and cell-dependent and varied according to the material used. Thus, the surface chemistry of graphene plays a critical role in its biocompatibility. Non-cancerous cells had a higher sensitivity to GO cytotoxicity than cancer cells. R-GO was highly biocompatible to MRC-5 cells and for A549 cells had a minimal effect of cell viability. At 37C˚, GO and r-GO were moderately hemolyric at concentration of 125 µg/ml and highly haemolytic at concentration of 300 µg/ml. Exposure of cells to both graphene derivatives led to reactive oxygen species (RO5) generation without genotoxicity. GO, but not r-GO, led to autophagy in both cell lines, possibly inhibiting the PIP3-Akt/mTOR pathway. For both cell lines and at non-lethal concentrations, GO downregulated the expression of glycogen synthase kinase-3 (GSK-3ß). GO was also found to dysregulate both Wnt/b-catenin and Akt cell signalling pathways which are vital for cellular function. The finding relating to cell signalling provide an insight to the safety of GO which is important to its use in cancer therapy.
22
Albert, Kelsey Morgan. "Microporous Membrane-based Co-culture of Human Embryonic Stem Cells." VCU Scholars Compass, 2007. http://scholarscompass.vcu.edu/etd_retro/161.
Повний текст джерелаАнотація:
Transwell inserts with microporous membranes, available from multiple commercial sources, have been widely used for various mammalian cell culture applications, including the reduction of cell culture mixing. In this study, we examined the feasibility and functionality of using this technology for separating human embryonic stem cells (hESCs) from their respective feeder cells. We found that when hESCs were propagated on transwell inserts positioned directly above feeder cells grown in a separate dish, the hESCs could be maintained in an undifferentiated state for over 10 passages with no change in their basic pluripotent characteristics. In parallel with our transwell insert experiments, we also evaluated the ability of a new defined, xeno-free medium, HEScGRO, to enhance the animal-free characteristics of the transwell insert-based culture system. Results from our studies demonstrate that HEScGRO medium assists in maintaining the pluripotent characteristics of hESCs propagated in the transwell insert- based culture system. These combined results represent a significant development in properly segregating stem cells from their feeders, thus eliminating cell mixing, contamination, and providing the cells with a superior environment for nourishment and controlled self-renewal. Overall, this development in hESC propagation could have wide-reaching applications for self-renewal and differentiation studies within the field of stem cell biology.
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Surampudi, Vasudha. "POLYSACCHARIDE-BASED SHEAR THINNING HYDROGELS FOR THREE-DIMENSIONAL CELL CULTURE." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3872.
Повний текст джерелаАнотація:
The recreation of the complicated tissue microenvironment is essential to reduce the gap between in vitro and in vivo research. Polysaccharide-based hydrogels form excellent scaffolds to allow for three-dimensional cell culture owing to the favorable properties such as capability to absorb large amount of water when immersed in biological fluids, ability to form “smart hydrogels” by being shear-thinning and thixotropic, and eliciting minimum immunological response from the host. In this study, the biodegradable shear-thinning polysaccharide, gellan-gum based hydrogel was investigated for the conditions and concentrations in which it can be applied for the adhesion, propagation and assembly of different mammalian cell types in an unmodified state, at physiological conditions of temperature. Cell studies, to show successful propagation and assembly into three-dimensional structures, were performed in the range of hydrogels which were deemed to be optimum for cell culture and the cell types were chosen to represent each embryonic germ layer, i.e., human neural stem cells for ectoderm, human brain microvasculature cells for mesoderm, and murine β-cells for endoderm, along with a pluripotent cell line of human induced pluripotent stem cells, derived from human foreskin fibroblasts. Three-dimensional cell organoid models, to allow for gellan gum based bioprinting, were also developed using human induced pluripotent stem cells and human neural stem cells.
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Lenhart, Natalie Kay. "Nursing Leadership Influence on Evidence-Based Practice Culture and Integration." ScholarWorks, 2017. https://scholarworks.waldenu.edu/dissertations/3497.
Повний текст джерелаАнотація:
Translating research to practice takes 10-20 years or more and evidence-based practice (EBP) integration remains at 10%-20%, despite recommendations requiring EBP-guided decisions. EBP integration has been associated with up to 30% decreases in healthcare system spending, improved quality outcomes, and increased staff satisfaction. Nurse leaders are accountable for EBP enculturation, yet rate quality and safety as the highest priority and EBP as the lowest. This knowledge gap perpetuates low EBP integration rates and hinders EBP enculturation. Asking whether EBP facilitative interventions for nurse leaders increase scores on organizational culture and readiness, beliefs, and EBP use scales addressed the knowledge gap via this quality improvement, pre/posttest pilot project. Multiple frameworks guided the project: the nursing process, Lewin's change management model, the Johns Hopkins Nursing EBP model, and the Five Practices of Exemplary Leadership-® model. A comprehensive literature search validated the design using EBP facilitators: educational interventions, transformational leadership, strategic planning, and a systems perspective. Pre/posttest data garnered from 14 non-direct care nurse leaders on the Organizational Culture and Readiness for System-Wide Integration of EBP Scale, the EBP Beliefs Scale, and the EBP Implementation Scale was analyzed using 2-sample t tests. Individual questions on the scales revealed statistically significant differences correlating to the facilitative interventions, yet overall aggregate scores did not change significantly. The limited findings contribute to the existing body of knowledge, while positive social implications include resolving public health and safety issues, reversing fiscal irresponsibility, and overcoming resistance to change.
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Cearfoss, Christine. "Los Angeles Community-Based Associate Social Workers' Understanding of Culture and Therapy." ScholarWorks, 2019. https://scholarworks.waldenu.edu/dissertations/6538.
Повний текст джерелаАнотація:
Social workers have no clear professional guidelines about the application of culturally competent mental health service delivery. Without culturally competent mental health service delivery, clients from diverse cultures do not access needed mental health services and they experience less effective therapy treatment outcomes and overall disparity of service delivery throughout the therapeutic process. The purpose of this descriptive case study was to better understand how community-based social workers are delivering culturally competent services to clients. The theoretical framework for the study was multiculturalism and the primary research questions addressed how associate clinical social workers who provide in-home mental health services in Los Angeles deliver culturally competent services to their clients. Through 8 interviews with associate clinical social workers, this descriptive case study revealed that without clear direction on what culturally competent services are, or how to deliver them, social workers are using a combination of personal experience and personal culture, educational and practice knowledge, and in some cases no attention to culture, to meet the mental health needs of their clients. This study emphasized the need for an industry wide understanding of the term cultural competency, so it could serve as the frame of reference by which practice professional skill level could be assessed, practice protocols measured, and could lead to social change through greater access to counseling services for clients.
26
Hodgson, Allan. "The development of a culture-based tool to predict team performance." Thesis, Loughborough University, 2014. https://dspace.lboro.ac.uk/2134/16290.
Повний текст джерелаАнотація:
The effect of national culture on the performance of teams is becoming an increasingly important issue in advanced western countries. There are many interlinked reasons for this, including the increasing globalisation of companies and the use of joint ventures for the development of expensive platforms. A further issue relates to the export of complex sociotechnical systems, where a culture clash between designer/manufacturer and user can lead to significant problems. This report describes research work that was carried out to analyse the cultural factors that influence the performance of teams (including researchers, designers, operators and crews), and to determine whether these factors could be captured in a tool to provide assistance to team managers and team builders. The original point of interest related to the development of increasingly complex sociotechnical systems, for example nuclear power stations, oil refineries, offshore oil platforms, hospital systems and large transport aircraft. Answers that might be sought, in particular by the senior managers of global companies, include (1) the best teams (or best national locations) for fundamental research, industrial research & development, product/system improvement and other key activities, and (2) the implications for system performance and, as a result, for system design, of targeting an eastern Asian market, a South-American market, etc. A literature review was carried out of the effects of culture on team performance, of culture measures and tools and of task classifications; in addition, empirical evidence of the validity of measures and tools was sought. Significant evidence was found of the effects of culture on teams and crews, but no national culture-based team performance prediction tools were found. Based on the results of the literature review, Hofstede's original four-dimension cultural framework was selected as the basis for the collection and analysis of empirical data, including the results of studies from the literature and the researcher s own empirical studies. No team or task classification system was found that was suitable for the purposes of linking culture to team performance, so a five-factor task classification was developed, based on the literature review, to form the basis of the initial modelling work. A detailed analysis of results from the literature and from the author s pilot studies revealed additional culture-performance relationships, including those relating to cultural diversity. Three culture-performance models were incorporated into software tools that offered performance prediction capabilities. The first model was primarily a test bed for ideas; the second model incorporated a task/behavioural approach which achieved limited success; the third and final model was evaluated against a range of team and crew performance data before being tested successfully for acceptability by users. The research results included the discovery that the effects of cultural diversity must be sought at the individual cultural dimension level not at the composite level, that the effects of national culture on team performance are consistent and strong enough to be usefully captured in a predictive culture tool and that the relationship between culture and behaviour is moderated by contextual factors.
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Bacevičiūtė, Rasa. "Prekės ženklo vertė vartotojui: tarpkultūrinis aspektas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20140626_184726-25179.
Повний текст джерелаАнотація:
Prekės ženklo vertės vartotojui kūrimo procesas tarptautinėje terpėje yra sudėtingas, tačiau šiuo atveju svarbiausia įvertinti egzistuojančius kultūrinius skirtumus. Literatūroje pasigendama nuoseklaus teorinio kultūrinių veiksnių įtakos prekės ženklo vertės vartotojui kūrime, pagrindimo. Vartojimo kultūriniai ypatumai skatina ieškoti naujų metodų, skirtų vartotojų prioritetams tirti. Dėl to ne visuomet prekės ženklo vertė vartotojui suvokiama taip, kaip tikisi ją kuriantys marketingo specialsitai. Darbo tikslas – atlikus teorines prekės ženklo vertės vartotojui tarpkultūriniu aspektu studijas, parengti kultūros ir prekės ženklo vertės dimensijų sąsajų modelį bei patikrinti jį empiriškai. Darbo tikslui pasiekti keliami uždaviniai: 1.Išanalizuoti prekės ženklo vertės vartotojui tarpkultūriniu aspektu problematikos teorinį reikšmingumą. 2.Pateikti prekės ženklo vertės vartotojui sampratą ir jos dedamąsias. 3.Identifikuoti tarptautinio prekės ženklo vertę lemiančius veiksnius. 4.Aptarti šalių kultūrų svarbą prekės ženklo vertei vartotojui. 5.Sukurti kultūros ir prekės ženklo vertės dimensijų sąsajų modelį. 6.Atlikti empirinį tyrimą pagal teorinėje dalyje parengtą modelį. Darbo rezultatai: -Teoriniu požiūriu išanalizuota ir susisteminta mokslinė literatūra apie prekės ženklo vertę vartotojui ir jos dedamąsias, prekės ženklo vertės kūrimo ypatumus tarptautiniu mastu, kultūrinių veiksnių įtaką prekės ženklo vertei vartotojui, apibrėžiama šalių kultūrų bei kultūrinių vertybių svarba... [toliau žr. visą tekstą]This master work examines consumer – based brand equity in cross – cultural aspect. Objective of master’s work is to execute theoretical studies of consumer – based brand equity in cross – cultural aspect and to check these studies empirically. The master work consist of three main parts. The first part of the master work includes theoretical analysis of a brand, consumer – based brand equity, the main factors of international branding and branding in different cultures, cultural differences and models of cultural dimensions. The second part of the master work includes theoretical approach of cross-cultural factors, which influence consumer - based brand equity and, according to empirical studies, conducted on consumer – based brand equity and cultural values, constructed theoretical brand value and culture chain based model. The third part of the master work consists of methodical rules and the empirical research, the purpose, task and the suggestions of the research; result analysis of the qualitative and quantitative data results; generalization of the research results, giving suggestions and recommendation. This master work presents theoretical and empirical researches results and recommendations. There are used 11 tables, 37 pictures and 5 additions in order to illustrate the theoretical and empirical results in master work. There are used 75 sources of scientific literature in Lithuanian and English in master work.
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Van, Niekerk Johannes Frederick. "Establishing an information security culture in organizations : an outcomes based education approach." Thesis, Nelson Mandela Metropolitan University, 2005. http://hdl.handle.net/10948/164.
Повний текст джерелаАнотація:
Information security is crucial to the continuous well-being of modern orga- nizations. Humans play a signfiicant role in the processes needed to secure an organization's information resources. Without an adequate level of user co-operation and knowledge, many security techniques are liable to be misused or misinterpreted by users. This may result in an adequate security measure becoming inadequate. It is therefor necessary to educate the orga- nization's employees regarding information security and also to establish a corporate sub-culture of information security in the organization, which will ensure that the employees have the correct attitude towards their security responsibilities. Current information security education programs fails to pay su±cient attention to the behavioral sciences. There also exist a lack of knowledge regarding the principles, and processes, that would be needed for the establishment of an corporate sub-culture, specific to information security. Without both the necessary knowledge, and the desired attitude amongst the employee, it will be impossible to guarantee that the organi- zation's information resources are secure. It would therefor make sense to address both these dimensions to the human factor in information security, using a single integrated, holistic approach. This dissertation presents such an approach, which is based on an integration of sound behavioral theories.
29
Rhodes, Theresa M. "Listen up a content-based approach to integrate listening and culture /." Muncie, Ind. : Ball State University, 2009. http://cardinalscholar.bsu.edu/688.
Повний текст джерела
30
Luft, Stephen. "Language classroom risk-taking behavior in a performed culture-based program." Connect to resource, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1229701363.
Повний текст джерела
31
Ma, Shaohua. "Routes to controlled microenvironments for cell culture using droplet-based microfluidics." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648213.
Повний текст джерела
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Chang, Jui-Jung. "Preliminary Research on Taiwanese Art Curriculum Design Based On Visual Culture." VCU Scholars Compass, 2006. http://scholarscompass.vcu.edu/etd/1541.
Повний текст джерелаАнотація:
In recent years, art education has started an on-going discussion on the issue of visual culture. In the past few years this issue also becomes topical due to the necessity to improve art education in Taiwan. Currently, art education based on visual culture has become a very important concern in Taiwan. However, the concept of visual culture has its origin in foreign theories. In order for our art professional to remain independent, it is essential that Taiwanese art teachers begin to address the issue of how to properly incorporate the concept of visual culture into the design of our art curriculum, by applying an educational method entirely based on the unique experiences of Taiwan. Responding to this need, I have tried in my current research to investigate the curriculum design model that is based on visual culture theories, and to combine it with a concept of cultural education that is uniquely Taiwanese. My hope is to provide a curriculum model that is based on native Taiwanese visual culture, which might ease the educators' efforts of turning theory into practice.Therefore, in the present study I will: (1) investigate the importance of applying visual culture to art education; (2) explore the suitability of applying the theory of visual culture to the design of art curriculum in Taiwan; (3) derive from this research a set of curriculum design principles based on the ideas of visual culture art education, which can be applied and linked to the cultural characteristics of Taiwan.
33
Lu, Wei-Bin. "GFP-based sensing and state estimation in transgenic plant cell culture /." free to MU campus, to others for purchase, 2002. http://wwwlib.umi.com/cr/mo/fullcit?p3060120.
Повний текст джерела
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Seubsamarn, Kanoknon Cho Yoon Seonghee. "Tourist motivation to use homestays in Thailand and their satisfaction based on the destination's cultural and heritage based attribute." Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/5351.
Повний текст джерелаАнотація:
The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on January 14, 2010). Thesis advisor: Dr. Seonghee Cho. Includes bibliographical references.
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Devlin, Renee. "Responsible Adult Culture (RAC) cognitive and behavioral changes at a community-based correctional facility /." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1211297932.
Повний текст джерела
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Tsaturyan, Tamara. "Projects as Governance Resources at Project-Based Organizations : The case of Umeå2014 European Capital of Culture." Thesis, Umeå universitet, Företagsekonomi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-84819.
Повний текст джерелаАнотація:
This thesis discusses the challenges of modern organizations in their efforts of designing relevant project governance systems. To address the challenge the paper proposes using resource-based view on project-based organizations in order to evaluate and identify key governance resources. Given that prevailing rational and standardized models in project-related literature provide organizations with homogeneous resource-base, this paper invites attention to those resources, which have the potential to deliver unique character to the organizations. The thesis first discusses the relevance of exploring projects as governance resources at project-based organizations, next screens the projects through VRIO framework of resource-based theory. Derived intangible resources and organizational resources are further explored at a case study organization. The findings are analysed through complex adaptive systems theory, where intrinsic motivations appear as sources for emerging project governance systems, while principal trust serves as a resource for self-organization of projects and project governance unit.
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Amribt, Zakaria. "Macroscopic modelling of hybridoma cell fed-batch cultures with overflow metabolism: model-based optimization and state estimation." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209279.
Повний текст джерелаАнотація:
Monoclonal antibodies (MAbs) have an expanding market for use in diagnostic and therapeutic applications. Industrial production of these biopharmaceuticals is usually achieved based on fed-batch cultures of mammalian cells in bioreactors (Chinese hamster ovary (CHO) and Hybridoma cells), which can express different kinds of recombinant proteins. In order to reach high cell densities in these bioreactors, it is necessary to carry out an optimization of their production processes. Hence, macroscopic model equations must be developed to describe cell growth, nutrient consumption and product generation. These models will be very useful for designing the bioprocess, for developing robust controllers and for optimizing its productivity.This thesis presents a new kinetic model of hybridoma cell metabolism in fed batch culture and typical illustration of a systematic methodology for mathematical modelling, parameter estimation and model-based optimization and state estimation of bioprocesses.
In the first part, a macroscopic model that takes into account phenomena of overflow metabolism within glycolysis and glutaminolysis is proposed to simulate hybridoma HB-58 cell cultures. The model of central carbon metabolism is reduced to a set of macroscopic reactions. The macroscopic model describes three metabolism states: respiratory metabolism, overflow metabolism and critical metabolism. The model parameters and confidence intervals are obtained via a nonlinear least squares identification. It is validated with experimental data of fed-batch hybridoma cultures and successfully predicts the dynamics of cell growth and death, substrate consumption (glutamine and glucose) and metabolites production (lactate and ammonia). Based on a sensitivity analysis of the model outputs with respect to the parameters, a model reduction is proposed.
In the next step, the effort is directed to the maximization of biomass productivity in fed-batch cultures of hybridoma cells based on the overflow metabolism model. Optimal feeding rate, on the one hand, for a single feed stream containing both glucose and glutamine and, on the other hand, for two separate feed streams of glucose and glutamine are determined using a Nelder-Mead simplex optimization algorithm. Two different objective functions (performance criteria) are considered for optimization; the first criterion to be maximized is the biomass productivity obtained at the end of the fed-batch culture, the second criterion to be minimized is the difference between global substrate consumption and the maximum respiratory capacity.
The optimal multi exponential feed rate trajectory improves the biomass productivity by 10% as compared to the optimal single exponential feed rate. Moreover, this result is validated by the one obtained with the analytical approach in which glucose and glutamine are fed to the culture so as to control the hybridoma cells at the critical metabolism state, which allows maximizing the biomass productivity. The robustness analysis of optimal feeding profiles obtained with different optimization strategies is considered, first, with respect to parameter uncertainties and, finally, with respect to model structure errors.
Finally, the overflow metabolism model is used to develop an extended Kalman filter for online estimation of glucose and glutamine in hybridoma cell fed-batch cultures based on the considered available measurements (biomasses (on-line), lactate and ammonia (on-line or off-line)). The observability conditions are examined, and the performances are analysed with simulations of hybridoma cell fed-batch cultures. Glutamine estimation sensitivity is enforced by minimizing a cost function combining a usual least-squares criterion with a state estimation sensitivity criterion.
Doctorat en Sciences de l'ingénieur
info:eu-repo/semantics/nonPublished
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Parisi, Ann Margaret. "Investigation of Secondary Metabolite Production in Selected Australian Native Species via Plant Cell Suspension Culture." Thesis, Griffith University, 2006. http://hdl.handle.net/10072/366129.
Повний текст джерелаАнотація:
Natural products and natural-product-derived substances comprised about
35% of the total pharmaceuticals market volume of US$230 billion in 1996
(Wessjohann, 2000). The success of natural-based drugs can be attributed to
nature’s ability to induce effects by chemical means and many of these chemicals
are able to pass species boundaries to cause an effect. Since plant secondary
metabolites have evolved in the interaction with other organisms, many of them
have interesting biological or therapeutical activities that are useful to man. In
addition to their intriguing chemistry a number of these compounds are
economically important, serving as pharmaceuticals, aromatics, fragrances,
stimulants, colours and pesticides.
Plant cell culture is viewed as a potential means of producing useful plant
products without the inherent problems associated with conventional agriculture.
Undifferentiated cell suspension cultures have the potential to produce varied
secondary metabolites by the alteration of culture conditions or addition of
chemicals to elicit expression of different metabolic pathways. Suitable substrate
compounds may be biotransformed to a desired product using plant cell cultures.
Biotransformation can produce compounds that can then be replicated by
synthetic means or produce novel compounds that have previously not been
identified or recognised as important. This thesis describes the initiation of plant
suspension cultures for the purposes of examining the production of secondary
metabolites of selected Australian native rainforest species.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Science
Science, Environment, Engineering and Technology
Full Text
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Devlin, Renee S. "Responsible Adult Culture: Cognitive and Behavioral Changes at a Community-Based Correctional Facility." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1211297932.
Повний текст джерела
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Bowden, Adelle. "Maximising the impact of evidence-based medicine on equine health and welfare." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/52194/.
Повний текст джерелаАнотація:
Evidence-based veterinary medicine (EVM) has the potential to improve welfare of equine patients in primary care practice by ensuring that decision making at the point of care is underpinned by credible research. Patient focussed care requires consideration of a combination of evidence, veterinary judgement, experience and owner associated variables to ensure the best possible outcomes for the animal, whilst maximising the impact of EVM on equine health and welfare. A holistic approach to development and implementation of EVM, that involves horse owners, other equine stakeholders and veterinary surgeons, will ensure that all parties involved in decision making on behalf of equine patients are engaged in evidence-based decisions. The central aim of the thesis was to determine how advances in evidence-based veterinary medicine had the potential to achieve the greatest impact on equine health and welfare. The objectives were to: • Review methods of implementing evidence-based medicine in clinical practice and determine their suitability for veterinary medicine. • Describe the prevalence and outcome of conditions seen for ‘out of hours’ primary assessment in equine practice. • Investigate horse owner perception, understanding and approach to the most common emergency conditions. • Identify paucities in the knowledge and resources available to equine owners and highlight the information required to implement and facilitate evidence-based decision making. • Use an evidence-based approach to develop and disseminate an educational resource for horse owners to improve understanding of an emergency condition and equine welfare associated with that specific disease. The first section of the thesis aimed to identify a common emergency condition with a major welfare impact, which would become a focus for research in subsequent studies. A combination of methods were used in these chapters including a retrospective case study of ‘out of hours’ case records from two practices over a three year period, and a mixed methods online survey of opinions and experiences of equine yard owners. In the retrospective case study, colic (abdominal pain) was the most common condition seen as an ‘out of hours’ emergency at both veterinary practices, accounting for approximately a third of the ‘out of hours’ caseload. The three most common reasons for ‘out of hours’ visits for both practices were colic (35%), wounds (20%) and lameness (11%). The majority of cases seen ‘out of hours’ required a single treatment for resolution of the presenting problem (58%), whilst 26% needed multiple treatments. An online survey consisting of open and closed questions was distributed to UK livery yard owners. The survey investigated participants’ demographics and experiences, their opinion of the most common emergency conditions in the horse, and decision-making in emergency situations. Descriptive analysis of data included frequency ranking and categorisation of free text responses. There were 104 survey participants. The majority had kept horses for more than ten years (97%), and had previous experience of emergency conditions (99%), predominantly of colic (96%) and wounds (92%). Participants considered that the most common emergency conditions were colic (98%), wounds (49%) and fractures (22%), and the most concerning conditions were colic (94%), lameness (36%) and wounds (21%). Factors important in emergency decision making were: degree of pain, likelihood of condition resolving, and severity of disease. The results of these studies provided the impetus for using colic as the focus for the next phases of work within this thesis. The second section of the thesis investigated veterinary practitioners’ and horse owners’ approaches to horses with abdominal pain, with the aim of identifying where knowledge or resources were good or were variable or lacking. The subset of horses with abdominal pain from the ‘out of hours’ retrospective case series were explored to describe how these animals presented to veterinary surgeons, how they were diagnosed, treated and the outcomes. The clinical signs associated with a ‘critical’ outcome of colic were determined using univariate logistic regression. In the retrospective case series, 941 cases presented with signs of abdominal pain; 23.9% (n=225/941) cases were categorised as ‘critical’, and 18% (n=168/941) were euthanased. Fifteen variables from the case presentation significantly correlated with a critical case (p < 0.01) and were therefore incorporated into the multivariate model. The final multivariable model included three variables significantly associated with the likelihood of a case being classified as ‘critical’: increased heart rate (p < 0.01), abnormal mucous membrane colour (p < 0.01) and absence of borborygmi in at least one quadrant (p < 0.01). These were considered to be essential clinical parameters associated with the differentiation of critical cases of colic, and therefore should be included in the veterinary assessment of the condition. To investigate horse owners’ opinions and experiences, a mixed methods survey was distributed to investigate owner knowledge, experience and approaches to colic through open and closed questions and clinical scenarios. The survey also aimed to highlight knowledge and resource gaps that may affect the welfare and outcomes of horses with abdominal pain. The horse owner survey identified that owners had variable and often limited knowledge of colic and were frequently poor at recognising some of the clinical and behavioural manifestations of the condition. This study also identified the need for an all-encompassing educational resource for horse owners underpinned by scientific evidence in an accessible and functional format. Developing evidence-based resources for veterinary practitioners and horse owners was beyond the scope of what could be achieved within this thesis. Educational colic resources for horse owners (‘REACT’) were collaboratively developed and disseminated by Nottingham Equine Colic Project (including the author of the thesis) and The British Horse Society. Current evidence and literature on methods of incorporating evidence into clinical decision making were reviewed within the thesis, specifically accounting for the difficulties faced in veterinary medicine. Future work is needed to develop evidence-based resources on colic for veterinary surgeons to ensure that scientifically underpinned decisions are made at the point of care. The work contained within this thesis highlighted the importance of the owner in the recognition of disease and instigation of veterinary intervention. It is crucial that both equine owners and veterinary surgeons are considered in equal measure when investigating decision making on behalf of a horse. Owners are the gatekeepers of animal care and welfare and therefore there is a requirement that they are included in research and the development of educational material. Veterinary directed studies are commonplace, however the benefits of such research are questionable if the horse owner does not present their animal for veterinary attention. The legacy of this study will likely be the inclusion of horse owners in the development of evidence-based educational campaigns.
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Osborne, Sonya Ranee. "Testing the effectiveness of a Practice Development intervention on changing the culture of evidence based practice in an acute care environment." Thesis, Queensland University of Technology, 2009. https://eprints.qut.edu.au/31051/1/Sonya_Osborne_Thesis_Vol_2.pdf.
Повний текст джерелаАнотація:
In this age of evidence-based practice, nurses are increasingly expected to use research evidence in a systematic and judicious way when making decisions about patient care practices. Clinicians recognise the role of research when it provides valid, realistic answers in practical situations. Nonetheless, research is still perceived by some nurses as external to practice and implementing research findings into practice is often difficult. Since its conceptual platform in the 1960s, the emergence and growth of Nursing Development Units, and later, Practice Development Units has been described in the literature as strategic, organisational vehicles for changing the way nurses think about nursing by promoting and supporting a culture of inquiry and research-based practice. Thus, some scholars argue that practice development is situated in the gap between research and practice. Since the 1990s, the discourse has shifted from the structure and outcomes of developing practice to the process of developing practice, using a Practice Development methodology; underpinned by critical social science theory, as a vehicle for changing the culture and context of care. The nursing and practice development literature is dominated by descriptive reports of local practice development activity, typically focusing on reflection on processes or outcomes of processes, and describing perceived benefits. However, despite the volume of published literature, there is little published empirical research in the Australian or international context on the effectiveness of Practice Development as a methodology for changing the culture and context of care - leaving a gap in the literature. The aim of this study was to develop, implement and evaluate the effectiveness of a Practice Development model for clinical practice review and change on changing the culture and context of care for nurses working in an acute care setting. A longitudinal, pre-test/post-test, non-equivalent control group design was used to answer the following research questions: 1. Is there a relationship between nurses' perceptions of the culture and context of care and nurses' perceptions of research and evidence-based practice? 2. Is there a relationship between engagement in a facilitated process of Practice Development and change in nurses' perceptions of the culture and context of care? 3. Is there a relationship between engagement in a facilitated process of Practice Development and change in nurses' perceptions of research and evidence-based practice? Through a critical analysis of the literature and synthesis of the findings of past evaluations of Nursing and Practice Development structures and processes, this research has identified key attributes consistent throughout the chronological and theoretical development of Nursing and Practice Development that exemplify a culture and context of care that is conducive to creating a culture of inquiry and evidence-based practice. The study findings were then used in the development, validation and testing of an instrument to measure change in the culture and context of care. Furthermore, this research has also provided empirical evidence of the relationship of the key attributes to each other and to barriers to research and evidence-based practice. The research also provides empirical evidence regarding the effectiveness of a Practice Development methodology in changing the culture and context of care. This research is noteworthy in its contribution to advancing the discipline of nursing by providing evidence of the degree to which attributes of the culture and context of care, namely autonomy and control, workplace empowerment and constructive team dynamics, can be connected to engagement with research and evidence-based practice.
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Garcia, Arcos Marcos 1963. "Evaluation of coconut coir-based media in transplant production." Thesis, The University of Arizona, 1997. http://hdl.handle.net/10150/278591.
Повний текст джерелаАнотація:
Each year the horticultural industry is demanding increasing amount of high quality soilless media. To meet the need this study evaluated coconut coir as an organic component in substrates. Asian and different Mexican coir-based media were compared to sphagnum peat to characterize coir suitability in tobacco transplant growth. These organic components were combined in different proportions with inorganic constituents to create three water holding capacity levels. The organic components, standardized in their capability to hold water, were evaluated by four growth parameters: leaf area, transplant height, dry weight, and total nitrogen concentration. Uncompressed, aged, and leached coir-based media produced transplants equivalent to those produced in peat-based media and were superior to those of either compressed or unleached coirs. Both extreme compression and high chloride concentration appear to affect coir suitability in growing transplants. Two Mexican leached coir and sand combinations produced the highest transplant quality under the conditions of this study.
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Jenson, Jennifer J. "Girls ex machina, a school-based study of gender, culture and technology." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0025/NQ51876.pdf.
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Cheema, Munira. "The production and reception of gender-based content in Pakistani television culture." Thesis, University of Sussex, 2015. http://sro.sussex.ac.uk/id/eprint/54446/.
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Lei, Jennifer. "The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications." Diss., Georgia Institute of Technology, 2016. http://hdl.handle.net/1853/54949.
Повний текст джерелаАнотація:
Mesenchymal stem cells are multipotent cells that have the ability to differentiate down multiple lineages as well as secrete trophic and anti-inflammatory factors. These qualities make MSCs a promising cell source for cell-based therapies to treat a variety of injuries and pathologies. Biomaterials are often used to control and direct stem cell behavior by engineering a desired environment around the cells. Recent research has focused on using the naturally derived sulfated glycosaminoglycan (GAG), heparin as a biomaterial due to its negative charge and ability to sequester and bind positively charged growth factors. Engineering a heparin coating that can mimic the native heparan sulfate proteoglycan structure found at cell surfaces can be used as a novel platform to present GAGs to cells to direct cell behavior. The overall goal of this dissertation was to develop GAG-based coatings on MSC spheroids in order to study the role of heparin and its derivatives on MSC culture applications. To investigate the role of heparin in coating form on MSC behavior, the ability of the coating to sequester positively charged growth factors was characterized. Given the role of sulfation in the negative charge density of heparin and growth factor interactions, a desulfated heparin coating was develop and used to examine how presentation of coatings with native and no sulfation levels could potentiate response to growth factors in the surrounding environment. Additionally, heparin and growth factor binding in coating presentation was explored to develop a novel platform to assemble MSC-based microtissues. Together these studies provided valuable insight into a novel approach to direct cell behavior by engineering a coating that harnesses heparin interactions with the surrounding environment.
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Alsulami, Naif Mastoor A. "Transforming Saudi mathematics education culture: An arts-based critical auto-ethnographic inquiry." Thesis, Curtin University, 2014. http://hdl.handle.net/20.500.11937/2584.
Повний текст джерелаАнотація:
Pre-service mathematics teachers in Saudi Arabia learn about student-centred teaching approaches to use in their professional teaching practice. However, many are not interested in implementing those approaches. Based on an Arts-based critical auto/ethnographic inquiry, I propose that Saudi future mathematics teachers need to engage in ‘transformative learning’; to think reflectively and critically about their own learning experiences, their beliefs about teaching and learning, their personal epistemology and their view about the nature of mathematics.
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Dorrington, Tarquin. "Recombinant antimicrobials for feed based delivery in aquaculture /." View online ; access limited to URI, 2005. http://0-digitalcommons.uri.edu.helin.uri.edu/dissertations/AAI3188058.
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Bussey, Jennifer Amy. "The Implications of National Culture on American Knowledge Work Teams: A Model of a Collaborative Corporate Culture to Support Team Functioning." Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc2257/.
Повний текст джерелаАнотація:
In order to remain competitive, many American businesses implement team-based work strategies. In many cases, however, teams fail in American organizations, which may be in part due to a conflict between American culture and the cooperative environment necessary for teams to function effectively. By comparing the literature regarding American culture, challenges faced by teams, and then corporate culture, it becomes evident that there are aspects of American culture that pose challenges but also that an appropriate corporate culture can mediate some areas of incompatibility. A collaborative corporate culture can induce cooperation among employees without asking employees to work in a manner that is counterintuitive, thus gaining the benefits of teams.
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Aref, Sepideh, and Foteini Bourzikou. "Heading towards an unknown culture : The adjustment of expatriates in Greece based on their selection and cross-cultural training." Thesis, Uppsala universitet, Företagsekonomiska institutionen, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-447347.
Повний текст джерелаАнотація:
International borders are dissolving as a result of globalization, and cross-cultural interactions are becoming a regular part of modern business. Many firms are expanding internationally, necessitating the need to send workers, known as expatriates, abroad. Being able to operate and adjust in a foreign country requires certain skills and traits. Not being able to adjust well in the host country has many times led expatriates to fail their international mission, which negatively affects the expatriate as well as the firm. Previous studies suggest that by selecting and offering a proper cross-cultural training (CCT), expatriates should have an easier time adjusting in the country they move to. Thus, leading to a successful international assignment. Research on selection and CCTs has predominantly focused on creating frameworks and finding variables that contribute to expatriates’ adjustment. However, there are still unclarities and disagreements regarding the effects of selection and CCT on the expatriates’ in-country adjustment. Therefore, it is important to understand whether expatriates perceive these processes helpful for their adjustment. Moreover, studies that have implemented these frameworks and variables into practicality for Asian or African host countries, thus leaving a gap in Europe and its emerging countries. As such, this study will contribute to this gap to further understand how selection and CCT affect the expatriate’s adjustment in Greece. A literature is presented with the relevant theories that exist in the field of expatriate selection, cross-cultural training and adjustment, and additionally cultural shock theories. With the aim of collecting data for this study, a qualitative method was approached, where 12 in-depth interviews were conducted on expatriates working in Greece. The interview covered topics of how they perceive their selection process, the CCT they received before their mission and their adjustment in the host country. General findings were that the classical selection and CCT theories and processes are still applicable up to this day. An additional finding to these theories was that the expatriates do not favor CCT processes. Moreover, it was observed that the perceived cultural differences between the home and host country of the expatriate is the main factor that is affected by the selection and CCT. Finally, the researchers suggest further research to be conducted for several factors concerning the in-country adjustment.
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Morakul, Supitcha. "Cultural Influences on the ABC Implementation Under Thailand's Environment." Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc279207/.
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