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1
Chapelain, Brigitte. "De nouvelles médiations numériques au service de la culture augmentée." Hermès 61, no. 3 (2011): 106. http://dx.doi.org/10.3917/herm.061.0106.
2
CHAPELAIN, Brigitte. "De nouvelles médiations numériques au service de la culture augmentée (encadré)." Hermès, no. 61 (2011): , [ p.]. http://dx.doi.org/10.4267/2042/45513.
3
Favre-Brun, Aurélie, and Livio De Luca. "De l'acquisition 3D à la réalité augmentée : le cas de l'église de la chartreuse pontificale de Villeneuve-lès-Avignon (Gard)." Revue Française de Photogrammétrie et de Télédétection, no. 196 (April 15, 2014): 52–58. http://dx.doi.org/10.52638/rfpt.2011.37.
Анотація:
La chartreuse de Villeneuve-lès-Avignon (Gard), fondée au XIVème siècle par le pape Innocent VI, a subi de nombreux bouleversements tout au long de son existence, à l'image de son église. Construite en plusieurs temps, elle a atteint son apogée au XVIIIème siècle, avant d'être vendue puis divisée en lots et occupée par la population pauvre locale. Le XXème siècle marque sa renaissance à travers les expropriations et les restaurations. Elle est aujourd'hui un site touristique et un lieu de résidence pour artistes. Dans le cadre du programme 3D[Monuments] piloté par le Ministère de la Culture et de la Communication, l'église a été le théâtre de campagnes de relevés numériques et photographiques visant à reconstruire en 3D son état actuel. Une restitution hypothétique de son état postrévolutionnaire, basée sur les nombreuses et diverses sources documentaires, a également été proposée. Une base de données contenant la documentation liée aux objets 3D a été mise en place. L'élaboration de la maquette 3D a conduit l'équipe à s'interroger sur la fiabilité des informations transmises par les sources documentaires. La chartreuse a ainsi été choisie comme terrain d'expérimentation privilégié pour l'étude de l'incertitude des données documentaires réalisée dans le cadre d'une thèse de doctorat.
4
Brault, Jean-Rémi. "Pivot, Bernard. Le métier de lire. Réponses à Pierre Nora; d’Apostrophes à Bouillon de culture. Nouvelle édition augmentée. [Paris] : Gallimard [2001]. 347 p. (Collection Folio, 3552)." Documentation et bibliothèques 48, no. 1 (2002): 29. http://dx.doi.org/10.7202/1030473ar.
5
Bushinsky, D. A. "Effects of parathyroid hormone on net proton flux from neonatal mouse calvariae." American Journal of Physiology-Renal Physiology 252, no. 4 (April 1, 1987): F585—F589. http://dx.doi.org/10.1152/ajprenal.1987.252.4.f585.
Анотація:
Bone mineral buffers protons during acute metabolic acidosis; whether parathyroid hormone (PTH) augments proton buffering is controversial. To determine whether PTH augments proton buffering by bone, we cultured neonatal mouse calvariae with or without PTH (10(-8) M) for 3 h in medium that was physiologically acid (pH approximately 7.20), neutral (pH approximately 7.40), or alkaline (pH approximately 7.60). Over the entire pH range studied there was less influx of protons into calvariae treated with PTH than into control calvariae, indicating that PTH does not augment but instead inhibits proton buffering by bone. To determine whether chronic exposure to PTH is necessary to augment proton buffering, calvariae were incubated with PTH for 24 h before a 3-h culture. Calcium efflux from calvariae exposed to PTH (10(-8) M) for 24 h exceeded that of controls. When these same calvariae were recultured for 3 h in fresh medium, PTH-treated and control calvariae behaved similarly, with net efflux of protons into acid, neutral, and alkaline media. Regardless of whether PTH is added at the time of exposure to acid medium or 24 h before calvariae cultured with PTH do not buffer protons to a greater extent than controls.
6
Hashimoto, Shin-ichi, Muneo Yamada, Kazuo Motoyoshi, and Kiyoko S. Akagawa. "Enhancement of Macrophage Colony-Stimulating Factor–Induced Growth and Differentiation of Human Monocytes by Interleukin-10." Blood 89, no. 1 (January 1, 1997): 315–21. http://dx.doi.org/10.1182/blood.v89.1.315.
Анотація:
Abstract Interleukin-10 (IL-10) has been reported to be a negative cytokine for monocytes/macrophages. In the present study, we showed that IL-10 is rather a positive cytokine and augments the growth and differentiation of human monocytes stimulated with macrophage colony-stimulating factor (M-CSF ). Highly purified adherent human monocytes were cultured for 7 days with M-CSF in the presence or absence of IL-10. The number of recovered cells increased in the culture of monocytes with M-CSF + IL-10 compared to the culture with M-CSF alone. IL-10 alone was not enough to maintain the survival and differentiation of monocytes into macrophages. Morphological change cultured in M-CSF was also accelerated by addition of IL-10, and macrophages cultured in M-CSF + IL-10 were more elongated compared to macrophages cultured with M-CSF alone. Binding of 125I-M-CSF to monocytes incubated with M-CSF + IL-10 was about 1.7-fold higher than that to monocytes incubated with M-CSF alone. In accordance with the binding study, Northern blot analysis showed that the levels of the expression of c-fms, M-CSF receptor, mRNA in macrophages cultured in M-CSF + IL-10 were higher than that in macrophages cultured in M-CSF alone. Macrophages cultured in M-CSF + IL-10 expressed higher level of FcγRI, II, III, and showed augmented Fcγ receptor mediated phagocytosis. The former also produced higher level of H2O2 and O−2 , when stimulated with zymosan, and of IL-6 when stimulated with lipopolysaccharide compared to the latter. These results taken together suggest that IL-10 augments the growth and differentiation of human monocytes cultured in M-CSF.
7
Hashimoto, Shin-ichi, Muneo Yamada, Kazuo Motoyoshi, and Kiyoko S. Akagawa. "Enhancement of Macrophage Colony-Stimulating Factor–Induced Growth and Differentiation of Human Monocytes by Interleukin-10." Blood 89, no. 1 (January 1, 1997): 315–21. http://dx.doi.org/10.1182/blood.v89.1.315.315_315_321.
Анотація:
Interleukin-10 (IL-10) has been reported to be a negative cytokine for monocytes/macrophages. In the present study, we showed that IL-10 is rather a positive cytokine and augments the growth and differentiation of human monocytes stimulated with macrophage colony-stimulating factor (M-CSF ). Highly purified adherent human monocytes were cultured for 7 days with M-CSF in the presence or absence of IL-10. The number of recovered cells increased in the culture of monocytes with M-CSF + IL-10 compared to the culture with M-CSF alone. IL-10 alone was not enough to maintain the survival and differentiation of monocytes into macrophages. Morphological change cultured in M-CSF was also accelerated by addition of IL-10, and macrophages cultured in M-CSF + IL-10 were more elongated compared to macrophages cultured with M-CSF alone. Binding of 125I-M-CSF to monocytes incubated with M-CSF + IL-10 was about 1.7-fold higher than that to monocytes incubated with M-CSF alone. In accordance with the binding study, Northern blot analysis showed that the levels of the expression of c-fms, M-CSF receptor, mRNA in macrophages cultured in M-CSF + IL-10 were higher than that in macrophages cultured in M-CSF alone. Macrophages cultured in M-CSF + IL-10 expressed higher level of FcγRI, II, III, and showed augmented Fcγ receptor mediated phagocytosis. The former also produced higher level of H2O2 and O−2 , when stimulated with zymosan, and of IL-6 when stimulated with lipopolysaccharide compared to the latter. These results taken together suggest that IL-10 augments the growth and differentiation of human monocytes cultured in M-CSF.
8
Rouquet, Tristan. "Belot (Robert), Lucien Rebatet. Le fascisme comme contre-culture, Rennes, Presses universitaires de Rennes, coll. « Histoire », 2015, 440 p., réédition refondue, revue et augmentée de Lucien Rebatet, un itinéraire fasciste (Paris, Le Seuil, 1994)." Politix 115, no. 3 (2016): 229. http://dx.doi.org/10.3917/pox.115.0229.
9
Okutsu, Mitsuharu, Kenji Ishii, Kai Jun Niu, and Ryoichi Nagatomi. "Cortisol-induced CXCR4 augmentation mobilizes T lymphocytes after acute physical stress." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 288, no. 3 (March 2005): R591—R599. http://dx.doi.org/10.1152/ajpregu.00438.2004.
Анотація:
The aim of this study was to elucidate the mechanism responsible for lymphopenia after exercise. Seven young healthy men volunteered for this study. Peripheral blood mononuclear cells (PBMC) were cultured with cortisol and analyzed for C-X-C motif chemokine receptor 4 (CXCR4) expression by flow cytometry. To determine the effects of exercise, subjects performed exhaustive cycling exercise. PBMC were cultured with plasma obtained before and after the cycling exercise. Alternatively, PBMC obtained before and after exercise were cultured without plasma or glucocorticoid to examine whether PBMC were primed in vivo for CXCR4 expression. We analyzed cortisol- or plasma-treated PBMC to determine their ability to migrate through membrane filters in response to stromal cell-derived factor 1α/CXCL12. Cortisol dose- and time-dependently augmented CXCR4 expression on T lymphocytes, with <6 h of treatment sufficient to augment CXCR4 on T lymphocytes. Postexercise plasma also augmented CXCR4 expression. Cortisol or postexercise plasma treatment markedly enhanced migration of T lymphocytes toward CXCL12. Augmentation of CXCR4 on T lymphocytes by cortisol or plasma was effectively blocked by the glucocorticoid receptor antagonist RU-486. Thus exercise-elicited endogenous cortisol effectively augments CXCR4 expression on T lymphocytes, which may account for lymphopenia after exercise.
10
Okada, M., M. Kitahara, S. Kishimoto, T. Matsuda, T. Hirano, and T. Kishimoto. "IL-6/BSF-2 functions as a killer helper factor in the in vitro induction of cytotoxic T cells." Journal of Immunology 141, no. 5 (September 1, 1988): 1543–49. http://dx.doi.org/10.4049/jimmunol.141.5.1543.
Анотація:
Abstract rIL-6/B cell stimulatory factor 2 was found to augment CTL generation from mature as well as immature human T cells stimulated with UV-treated allogeneic cells. rIL-6 also acted on peanut agglutinin-positive murine thymocytes and Lyt-2-positive splenic T cells to give rise to CTL. rIL-6 alone could not induce CTL generation, the presence of IL-2 during the early phase of culture period was found to be essential for the IL-6 activity in the induction of CTL. The effect of rIL-6 was not mediated by the induction of IL-2 inasmuch as rIL-6 did not augment IL-2 production in MLC and anti-IL-2 antibody could not neutralize IL-6 activity. rIL-6 augmented CTL generation even when added 72 h after the initiation of cultures. The enhancing activity of rIL-6 could be neutralized with anti-IL-6 antibodies even when added 72 h after the initiation of cultures. The present data indicate that IL-6 acts in the late phase of CTL generation.
11
PERILLI, LORENZO. "ILSETRAUT HADOT, Arts libéraux et philosophie dans la pensée antique: contribution à l'histoire de l'éducation et de la culture dans l'antiquité. Seconde èdition revue et considérablement augmentée. Paris: Libr. philosophique J. Vrin, 2005. 578 pp., ISBN 2711618234." Nuncius 22, no. 1 (January 1, 2007): 140–41. http://dx.doi.org/10.1163/221058707x00080.
12
Burgess, S. K., N. Sahyoun, S. G. Blanchard, H. LeVine, K. J. Chang, and P. Cuatrecasas. "Phorbol ester receptors and protein kinase C in primary neuronal cultures: development and stimulation of endogenous phosphorylation." Journal of Cell Biology 102, no. 1 (January 1, 1986): 312–19. http://dx.doi.org/10.1083/jcb.102.1.312.
Анотація:
Embryonic rat neurons cultured in defined medium, essentially in the absence of glia, were highly enriched in phorbol ester receptors. The neurons displayed a single class of phorbol 12,13-dibutyrate binding sites with a maximum binding capacity, after 10 d in culture, of 18.6 pmol/mg protein and an apparent dissociation constant of 7.1 nM. Phorbol ester binding sites were associated with protein kinase C, which represented a major protein kinase activity in primary neuronal cultures. Ca2+-phosphatidylserine-sensitive phosphorylation of endogenous substrates was more marked than that observed in the presence of cyclic AMP or Ca2+ and calmodulin. Phorbol ester receptors and protein kinase C levels were critically dependent on the culture age. Thus, about a 20-fold increase in binding sites occurred during the first week in culture and was accompanied by a corresponding increase in Ca2+-phosphatidylserine-sensitive protein phosphorylation in soluble neuronal extracts. These changes largely paralleled a similar rise in phorbol ester binding during fetal development in vivo. The apparent induction of phorbol ester receptors was specific relative to other cellular proteins and could be inhibited by cycloheximide or Actinomycin D. Phosphorylation of endogenous substrates in intact cultured neurons paralleled the age-dependent increase in protein kinase C. Furthermore, 32P incorporation into several major phosphoproteins was markedly augmented by treating the neuronal cultures with phorbol esters. Such phosphorylation events may provide a clue to the significance of protein kinase C in developing neurons.
13
Kariyone, Ai, Masashi Ikutani, Yoshinori Nagai, and Kiyoshi Takatsu. "Molecular mechanisms of Th1-mediated antigen cross-presentation: roles of Iigp1 and ingenol. (130.34)." Journal of Immunology 184, no. 1_Supplement (April 1, 2010): 130.34. http://dx.doi.org/10.4049/jimmunol.184.supp.130.34.
Анотація:
Abstract Antitumor immunity requires activation of CD8+ cytotoxic T cells (CTL) by antigen-presenting cells (APCs) through interaction with tumor antigen peptides on MHC class I molecules. Intracellular mechanisms on MHC class I-restricted soluble antigen presentation (cross-presentation) remain elusive. Peptide-25 of Ag85B induces Th1 cell response in I-Ab mice that facilitate CTL generation against unrelated Ovalbumin (OVA) peptide when co-immunized with OVA, indicating that Peptide-25 augments cross-presentation by APCs. In this presentation we will discuss about roles of Iigp1 and effects of various natural products on Th1-mediated enhancement of the cross-presentation. We evaluated the cross-presentation by 2-step culture system. At first, APCs were cultured with P25 TCR-Tg CD4+ T cells, Peptide-25, OVA or natural products for overnight. After the culture the OVA-pulsed APCs were harvested and cultured with OVA specific OT-I Tg CD8+ T cells for 3 days. The cross-presentation activity was evaluated by OT-1 proliferation and IFN-γ production. Results revealed an indispensable roles of IFN-γ and IFN-γ-inducible genes such as Iigp1 in the Th1-mediated cross-presentation. We also found that some of natural products such as ingenol derivatives augment the cross-presentation in an Th1-independent manner.
14
Rola-Pleszczynski, M., and I. Lemaire. "Leukotrienes augment interleukin 1 production by human monocytes." Journal of Immunology 135, no. 6 (December 1, 1985): 3958–61. http://dx.doi.org/10.4049/jimmunol.135.6.3958.
Анотація:
Abstract The effects of leukotrienes (LT) on production of interleukin 1 (IL 1) by human peripheral blood monocytes were examined. LTB4 enhanced IL 1 production by lipopolysaccharide (LPS)-stimulated monocytes twofold to threefold, and the most efficient concentrations of LTB4 were 10(-8) to 10(-7) M. LTD4 also enhanced IL 1 production, but to a lesser extent than LTB4. Adherence-purified, but otherwise unstimulated, human monocytes could also be induced to produce IL 1 in response to LTB4. Similarly, IL 1 production by monocytes stimulated with the known IL 1 inducers muramyl dipeptide, silica, or zymosan was also enhanced by LTB4. Inhibition of cyclooxygenase with use of indomethacin during IL 1 production by LPS-treated monocytes enhanced thymocyte response to IL 1, but LTB4 further enhanced IL 1 production when added to indomethacin-treated monocyte cultures. Neither LTB4 nor indomethacin had any direct effect on thymocyte proliferation. Optimal enhancement of IL 1 production occurred when LPS and LTB4 were present together at the initiation of the 24-hr monocyte culture. Significant enhancement was also observed, however, when monocyte cultures were either preincubated with LTB4 before addition of LPS or cultured with LPS alone for 3 hr before addition of LTB4. These results indicate that leukotrienes can modulate IL 1 production by human monocytes and suggest that they may play a role in IL 1-mediated functions of monocytes in inflammatory and immune reactions.
15
Ochoa, A. C., G. Gromo, B. J. Alter, P. M. Sondel, and F. H. Bach. "Long-term growth of lymphokine-activated killer (LAK) cells: role of anti-CD3, beta-IL 1, interferon-gamma and -beta." Journal of Immunology 138, no. 8 (April 15, 1987): 2728–33. http://dx.doi.org/10.4049/jimmunol.138.8.2728.
Анотація:
Abstract Peripheral blood lymphocytes (PBL) cultured in interleukin 2 (IL 2)-containing medium in conventional tissue culture develop the ability to lyse fresh tumor cells; such cells are referred to as lymphokine-activated killer (LAK) cells. LAK activity peaks by day 5 of culture and declines rapidly thereafter. We studied culture conditions and signals that allow for long-term culture and expansion of cells with LAK activity. By culturing cells at relatively low densities and regularly replenishing medium and recombinant IL 2 (r-IL 2), LAK function is significantly higher as compared with short-term cultures, and remains present for at least 21 days while cell numbers undergo an average 100-fold expansion. By activating these cultures with anti-CD3 (OKT3) monoclonal antibody and r-IL 2, an approximately 1000-fold expansion in the cell number is obtained with maintenance of comparable levels of LAK activity. The exogenous addition of beta interleukin 1 (beta-IL 1), interferon-beta (IFN-beta) or interferon-gamma (IFN-gamma) can augment the lytic activity of cell populations expanded by anti-CD3 plus r-IL 2. These approaches may enable the in vitro generation from individual donors of much greater numbers of LAK cells for adoptive immunotherapy than can now be obtained with the 3 to 5 day in vitro culture systems.
16
Aliotta, Jason M., Mandy Pereira, and Peter J. Quesenberry. "Tissue-Specific Gene Expression of Marrow Cells Co-Cultured with Various Murine Organs." Blood 112, no. 11 (November 16, 2008): 388. http://dx.doi.org/10.1182/blood.v112.11.388.388.
Анотація:
Abstract We have previously demonstrated that murine bone marrow cells co-cultured with lung opposite a cell-impermeable membrane express high levels of lung cell-specific genes (Aliotta et al, Stem Cells, 2007;25(9):2245–56). Greater changes in gene expression were noted in marrow cells co-cultured with radiation-injured lung. A factor smaller than the pore size of the cell-impermeable membrane (0.4μm) is responsible for these changes as cell-free conditioned media (CM) had a similar effect on co-cultured marrow cells. Lung-derived microvesicles were found to enter marrow cells in culture and may be among the factors responsible for these phenotypic changes. We wished to determine if these observations could be generalized to co-cultures using other murine organs and whether these changes in gene expression are tissue-specific. Whole bone marrow (WBM) cells were isolated from C57BL/6 mice and co-cultured with lung, liver, heart and WBM cells from C57BL/6 mice exposed to 1200 centigrey (cGy) of total body irradiation (TBI) or no radiation. Control WBM cells were co-cultured with no tissue (control). Co-cultured WBM cells were analyzed 7 or 14 days later by Real Time RT-PCR and fold difference in target gene expression was determined (relative to control cells). In addition, cell-free CM made from the same organs was co-cultured for 7 or 14 days with WBM cells which were then analyzed by RT-PCR. Alternatively, CM was analyzed for the presence of microvesicles by electron microscopy (EM) of ultracentrifuged (UCF) pelleted material. WBM co-cultured only with lung had increased gene expression of surfactant proteins A (Sp-A) and C (Sp-C, 89 and 334-fold increase vs. control, respectively) whereas WBM co-cultured only with brain had increased gene expression of Glial Fibrillary Acidic Protein (GFAP, 4.6-fold increase vs. control). Slight increases in Albumin expression were seen in all co-culture groups but expression was markedly elevated in WBM co-cultured with liver (162,657-fold increase vs. control). Expression of heart-specific markers, including Troponin I and T2, was seen in WBM co-cultured with heart but these levels were not significantly different from those of other co-culture groups. Radiation injury augmented expression of certain genes in co-cultured WBM, including Sp-A (1019 vs. 89-fold increase) in lung co-cultures and GFAP (24 vs. 4.6-fold increase) in brain co-cultures. WBM co-cultured with CM from all organs demonstrated similar changes in gene expression. In addition, pelleted material from UCF CM contained RNA that was specific to the tissue from which the CM was made. EM of UCF CM demonstrated numerous membranebound particles 50–200nm in size that were typical of microvesicles in appearance. These data suggest that changes seen in gene expression of co-cultured WBM are largely tissue-specific, depending on the tissue they are co-cultured with. Microvesicles released by various tissues in co-culture may be among the mediators responsible for the changes seen in WBM gene expression.
17
Zapata Cárdenas, María Isabel. "Aplicación en realidad aumentada para divulgación del patrimonio cultural." kepes 13, no. 14 (July 10, 2016): 33–59. http://dx.doi.org/10.17151/kepes.2016.13.14.3.
18
Kawano, M., H. Tanaka, H. Ishikawa, M. Nobuyoshi, K. Iwato, H. Asaoku, O. Tanabe, and A. Kuramoto. "Interleukin-1 accelerates autocrine growth of myeloma cells through interleukin-6 in human myeloma." Blood 73, no. 8 (June 1, 1989): 2145–48. http://dx.doi.org/10.1182/blood.v73.8.2145.2145.
Анотація:
Abstract Recombinant interleukin 1 alpha (rIL-1 alpha) augmented proliferation of freshly isolated myeloma cells as well as B-cell stimulatory factor 2 (BSF-2)/interleukin-6 (IL-6). Recombinant IL-1 alpha-induced proliferation was partially inhibited by anti-IL-6 antibody. In the culture supernatants of rIL-1 alpha-stimulated myeloma cells, IL-6 activities, which were measured by using an IL-6-dependent murine hybridoma clone, MH60.BSF2, were increased, when compared with those in the culture supernatants of nonstimulated myeloma cells. Furthermore, IL-6 messenger RNA (mRNA) expression was also augmented in IL-1 alpha- stimulated myeloma cells. Therefore rIL-1 alpha stimulates myeloma cells to produce IL-6, which consequently augments proliferation of myeloma cells. Thus, IL-1 can accelerate autocrine growth of myeloma cells through IL-6.
19
Kawano, M., H. Tanaka, H. Ishikawa, M. Nobuyoshi, K. Iwato, H. Asaoku, O. Tanabe, and A. Kuramoto. "Interleukin-1 accelerates autocrine growth of myeloma cells through interleukin-6 in human myeloma." Blood 73, no. 8 (June 1, 1989): 2145–48. http://dx.doi.org/10.1182/blood.v73.8.2145.bloodjournal7382145.
Анотація:
Recombinant interleukin 1 alpha (rIL-1 alpha) augmented proliferation of freshly isolated myeloma cells as well as B-cell stimulatory factor 2 (BSF-2)/interleukin-6 (IL-6). Recombinant IL-1 alpha-induced proliferation was partially inhibited by anti-IL-6 antibody. In the culture supernatants of rIL-1 alpha-stimulated myeloma cells, IL-6 activities, which were measured by using an IL-6-dependent murine hybridoma clone, MH60.BSF2, were increased, when compared with those in the culture supernatants of nonstimulated myeloma cells. Furthermore, IL-6 messenger RNA (mRNA) expression was also augmented in IL-1 alpha- stimulated myeloma cells. Therefore rIL-1 alpha stimulates myeloma cells to produce IL-6, which consequently augments proliferation of myeloma cells. Thus, IL-1 can accelerate autocrine growth of myeloma cells through IL-6.
20
Wittman, Emma, Neela Yar, Francesco De Seta, and Bryan Larsen. "In Vitro Exploration of Probiotic Bacteria Interactions with Candida Using Culture Techniques to Model Dysbiotic Conditions in Colonized Tissues." Pathogens 10, no. 3 (March 3, 2021): 289. http://dx.doi.org/10.3390/pathogens10030289.
Анотація:
Candida albicans overgrowth at various mucosal sites is an ongoing and complex clinical concern involving interactions with indigenous microbiota and therapeutic or preventive measures superimposed on the pathogen-microbiome interaction. In this paper we describe the use of quantitative flow cytometry (specific to the cytometer’s sample introduction mechanism) to explore the in vitro interaction between Candida albicans, probiotic lactobacilli and a topical vaginal therapeutic. Our central hypothesis was cytometric measurements of co-cultures of yeast and bacteria could provide a useful method for exploring the dynamics of different microbial species in culture, with and without inhibitors. Two commercial products were used as exemplars for this research, a vaginal antimicrobial gel and two species of probiotic lactobacillus intended or oral administration with crystalline bovine lactoferrin to augment the vaginal gel. The cytometer forward channel height parameter distinguished yeast from bacteria in co-culture experiments in the presence of a vaginal therapeutic gel or components of its formulation including EDTA, glycogen, polydextrose as well as the host defense factor, lactoferrin. Flow cytometry showed lactobacilli influenced yeast counts in co-culture, with the technique lending itself to wide-ranging test conditions including organisms, media composition and screening of various antimicrobials. Key findings: The proprietary vaginal gel augmented the effect of lactobacilli, as did EDTA and lactoferrin. Prebiotic compounds also enhanced Candida inhibition by lactobacilli. Propidium iodide (Fluorescence channel 3) discriminated between necrotic and non-necrotic yeast and bacteria in co-cultures under various culture conditions. This research demonstrates the value of flow cytometry to evaluate the population dynamics of yeast and bacteria in co-culture using a proprietary product and its components. We discuss both the limitations of the current study and describe how methods employed here would be transferrable to the investigation of organisms present in defined cultures or at body sites colonized by fungal species and the effects of therapeutics or probiotics on Candida.
21
Hristov Dobrev, Iliya, and Kosta Garov. "OPPORTUNITIES AND CHALLENGES OF AUGMENTED AND VIRTUAL REALITY IN EDUCATION." Education and Technologies Journal 14, no. 1 (August 1, 2023): 200–203. http://dx.doi.org/10.26883/2010.231.5077.
Анотація:
The development of information technology allows the integration of new innovative approaches into the modern learning environment. In the article „Opportunities and Challenges of Augmented and Virtual Reality in Education“ the author, as a longtime teacher of information technologies in lower secondary school, reveals the opportunities for using augmented and virtual reality in the school class. The applications Popar Toys, Augment Education, Solar System Scope, Breakfast launch, Atlas of Human Anatomy are described. Mozaik 3D, Google Arts & Culture and working with them. The author shares good practices from the implementation of augmented and virtual reality in the learning process and lists the opportunities and challenges of innovative and interactive teaching with their help.
22
Satria, Fajar, and Anita Fira Waluyo. "Aplikasi Pengenalan Senjata Tradisional Yogyakarta Berbasis Augmented reality." Jurnal Indonesia : Manajemen Informatika dan Komunikasi 5, no. 2 (May 10, 2024): 1111–20. http://dx.doi.org/10.35870/jimik.v5i2.588.
Анотація:
Yogyakarta, a city of culture, has a variety of cultures, including traditional weapons, but these are currently difficult to find. Research has shown that Augmented reality technology can educate the public about the culture of a region, such as the introduction of traditional Yogyakarta weapons through an application that displays 3D objects of traditional Yogyakarta weapons. By utilizing Augmented reality technology in the introduction of traditional Yogyakarta weapons, it becomes a material that is interesting to learn. This application can display 3D objects of traditional Yogyakarta weapons, so that the public can visualize traditional weapons that are currently quite difficult to find. The 3D objects were made based on data from a book published by the Ministry of Education and Culture. The target marker is made in the form of a QR code with a photo of a weapon in the middle, when the marker is detected, a 3D object will appear above it. The results of the testing showed that all features were able to run well.
23
Wallace, PM, JF MacMaster, JR Rillema, J. Peng, SA Burstein, and M. Shoyab. "Thrombocytopoietic properties of oncostatin M." Blood 86, no. 4 (August 15, 1995): 1310–15. http://dx.doi.org/10.1182/blood.v86.4.1310.bloodjournal8641310.
Анотація:
Oncostatin M (OM) is a 28-kD glycoprotein that exhibits a panoply of biologic effects. Based on histologic observations of increased splenic megakaryocytes in nude mice implanted with an OM-secreting cell line, the thrombocytopoietic properties of OM in mice were investigated in culture and in vivo. Alone, OM did not induce megakaryocytic colony formation, but in combination with murine interleukin-3 (IL-3), OM markedly enhanced colony formation. The effects of OM on colony formation were similar to those of IL-6. OM alone augmented acetylcholinesterase in short-term marrow cultures. In normal mice, the administration of OM augmented platelet counts without increasing other circulating blood cell counts. The increment in counts exceeded that observed with IL-6. The kinetics of the OM response suggested that maximal increases in platelets occurred 3 days after the cessation of OM administration, irrespective of the duration of administration. In irradiated mice, OM administration accelerated platelet recovery and prevented the decrease in red blood cells observed in irradiated control animals. The data show that OM behaves as a megakaryocytic maturation factor in vitro and augments platelet production in vivo. Based on these animal data, OM may have potential clinical utility as a thrombocytopoietic agent.
24
Stocker, K. M., L. Sherman, S. Rees, and G. Ciment. "Basic FGF and TGF-beta 1 influence commitment to melanogenesis in neural crest-derived cells of avian embryos." Development 111, no. 2 (February 1, 1991): 635–45. http://dx.doi.org/10.1242/dev.111.2.635.
Анотація:
In previous studies, we showed that neural crest (NC)-derived cells from embryonic quail dorsal root ganglia (DRG) and peripheral nerve (PN), which do not normally give rise to melanocytes, become committed to melanogenesis following treatment in culture with the phorbol ester drug 12-O-tetradecanoyl phorbol-13-acetate (TPA). These and other observations support the notion that melanocytes and Schwann cells are derived from a common bipotent intermediate in the neural crest lineage—the melanocyte/Schwann cell progenitor. In this study, we test the possibility that peptide growth factors found in the embryonic environment might act similarly to TPA to influence the fates of these cells. DRG and PN explants were cultured in medium supplemented with a variety of growth factors, and then the cultures were examined for the presence of pigment cells. We found that basic fibroblast growth factor (bFGF), but not various other growth factors, induced pigmentation in about 20% of these cultures. When low concentrations of TPA were included in the culture medium, bFGF augmented the TPA-induced pigmentation, significantly increasing the proportion of pigmented cultures. These effects of bFGF were age-dependent, and could be blocked by addition of a bFGF-neutralizing antibody to the culture medium. In contrast to these stimulatory effects of bFGF, transforming growth factor-beta 1 (TGF-beta 1) was found to inhibit the TPA- or bFGF-induced pigmentation of DRG cultures. These data suggest, therefore, that at least some NC-derived cells are responsive to bFGF and TGF-beta 1, and that these growth factors may play an important role in the control of NC cell fate.
25
Nakanishi, Shinobu, and Edwin M. Uyeki. "Benzamide on chondrocytic differentiation in chick limb bud cell culture." Development 85, no. 1 (February 1, 1985): 163–75. http://dx.doi.org/10.1242/dev.85.1.163.
Анотація:
Benzamide, an inhibitor of (ADP-ribose) transferase, augmented chondrocytic differentiation of chick limb bud mesenchymal cells in micromass cultures; the incorporation of 35SO42− into the trichloroacetic-acid-insoluble constituents of cell masses as well as the formation of cartilage nodules (Nishio, Nakanishi, Doull & Uyeki, 1983) occurred about 24h earlier than in untreated cultures and continued to be enhanced in benzamide-treated cultures of stage 23- to 24-chick limb bud cells. Benzamide also significantly increased cell proliferation. However, benzamide did not affect DNA and RNA syntheses except for one period: 24 to 30 h after the start of culture, RNA synthesis was stimulated. From 48h of culture, (ADP-ribose) transferase activity decreased daily in untreated cultures, whereas benzamide treatment diminished (ADP-ribose) transferase activity 24 h earlier. On the other hand, intracellular NAD levels increased daily in untreated cultures, and benzamide significantly increased the NAD levels above untreated cultures. ATP levels did not differ significantly during the culture period, and benzamide did not affect ATP levels.
26
Valdovinos, Jorge I. "Transparency as Ideology, Ideology as Transparency: Towards a Critique of the Meta-aesthetics of Neoliberal Hegemony." Open Cultural Studies 2, no. 1 (December 1, 2018): 654–67. http://dx.doi.org/10.1515/culture-2018-0059.
Анотація:
Abstract Along with the increasing commodification of all aspects of culture and the persistent aestheticisation of everyday life under late capitalism, there is an equally increasing longing for objectivity, immediacy, and trust. As the mediation of our everyday experiences augments, a generalised feeling of mistrust in institutions reigns; the sense of a need to bypass them increases, and the call for more “transparency” intensifies. As transparency manages to bypass critical examination, the term becomes a source of tacit social consensus. This paper argues that the proliferation of contemporary discourses favouring transparency has become one of the fundamental vehicles for the legitimation of neoliberal hegemony, due to transparency's own conceptual structure-a formula with a particularly sharp capacity for translating structures of power into structures of feeling. While the ideology of transparency promises a movement towards the abolition of unequal flows of information at the basis of relations of power and exploitation, it simultaneously sustains a regime of hyper-visibility based on asymmetrical mechanisms of accountability for the sake of profit. The solution is not “more” transparency or “better” information, but to critically examine the emancipatory potential of transparency at the conceptual level, inspecting the architecture that supports its parasitic logic.
27
Elmasry, M. N., E. J. Fox, and R. R. Rich. "Opposing immunoregulatory functions of CD8+ lymphocytes: a requirement for monocytes in suppressor cell induction." Journal of Immunology 137, no. 8 (October 15, 1986): 2468–77. http://dx.doi.org/10.4049/jimmunol.137.8.2468.
Анотація:
Abstract We have found that the induction of suppressor T cell (Ts) function in pokeweed mitogen (PWM)-stimulated cultures of peripheral blood mononuclear cels (PBMC) depends on the concentration of monocytes in inductive cultures. When cultured for 7 to 8 days in a physiologic concentration of monocytes (10 to 15%) suppressor cells differentiated with the surface phenotype CD8+ DR+ Leu-7- Leu-8+ Tp44-. Suppressor function, as assessed in secondary cultures of fresh PWM-stimulated CD4+ cells, was partially radiosensitive, and its induction could be partially inhibited by treatment with indomethacin. Culture supernatants of PWM-pulsed CD4+ cells could induce CD8+ suppressor cells only when monocytes were included in cultures for supernatant production. In marked contrast, if the monocyte concentration of PWM-stimulated T cells was reduced to less than 5%, CD8+ cells harvested from such cultures substantially augmented proliferation of PWM-stimulated CD4+ cells in assay cultures. Amplifier cells from monocyte-depleted cultures, were also DR+Tp44-. Suppressive activity of the CD8+ subset was restored simply by addition of monocytes to purified T cells, reconstituting the concentration present among unfractionated PBMC. Addition of IL 1 could not replace the requirement of monocytes for suppressor cell induction either in PWM-stimulated primary cultures or in culture supernatants. The data thus demonstrate that the regulatory activity of CD8+ lymphocytes is critically dependent on the conditions of cellular activation; at concentrations of monocytes normally present in peripheral blood, PWM stimulation induced potent suppressor activity, whereas under conditions of moderate monocyte depletion the regulatory activity of the same phenotypic subset of CD8+ cells was reversed. The experiments thus suggest that the functional consequences of an activating stimulus may depend on regulatory effects of monocytes in CD8+ suppressor cell induction.
28
Oktavia, Chaulina Alfianti, and Achmad Buyung Riyadi. "INTRODUCTION TO KALIMANTAN CULTURE USING AUGMENTED REALITY TECHNOLOGY." Cyberspace: Jurnal Pendidikan Teknologi Informasi 8, no. 1 (March 31, 2024): 14. http://dx.doi.org/10.22373/cj.v8i1.23028.
Анотація:
Central Kalimantan is a province consisting of 13 regencies and 1 city with the capital city of Palangkaraya. Many people, especially the younger generation, do not know the various cultures of Central Kalimantan such as traditional houses, clothes, traditional weapons, and so on. To facilitate the introduction of this culture an application is designed using Android-based Augmented Reality technology. Augmented Reality was designed using marker technology. From the test results, markers can be detected properly and can recognize and display Central Kalimantan cultural objects such as traditional houses, clothing, traditional weapons, and others. With the Augmented Reality application, it can make it easy for users to recognize the culture of Central Kalimantan. Based on the test results which included testing the AR Central Kalimantan application, the content of the AR Central Kalimantan application, and the display aspect obtained a score of 86%, it can be concluded that the application has been running well.
29
Koch, Raphael, Martin Demant, Thiha Aung, Annemarie Guentsch, Nina Diering, Bjoern Chapuy, Lorenz Truemper, and Gerald Wulf. "Exosome Mediated Wnt-Signaling Augments Side Population In Aggressive Lymphoma." Blood 122, no. 21 (November 15, 2013): 3010. http://dx.doi.org/10.1182/blood.v122.21.3010.3010.
Анотація:
Abstract Introduction Patients with aggressive B-cell lymphoma are treated in curative intention. However, some patients experience fatal relapse, originating from refractory lymphoma cells with the capacity for clonogenic regrowth. We here addressed repopulation capacity of lymphoma cell subpopulations and the mechanisms regulating the populational composition in the growing tumor. Material & Methods We identified side population (SP) cells in diffuse large B-cell lymphoma cell lines and patient samples with the DNA-binding dye Hoechst33342, analyzed clonogenicity in vitro and in vivo and screened for differentially expressed genes and DNA-methylation patterns. A GFP-containing lentiviral vector construct was used to keep track of side population cells cultured among mixed cultures of SP and nonSP cells. Manipulation of canonical wnt-signaling was performed by lentiviral sh-RNA constructs as well as pharmacological tankyrase-inhibition by XAV-939. In vitro data were supported by in vivo experiments using a chorioallantoic membrane-assay. Results Colony assays and suspension cultures of sorted SP and nonSP cells revealed restriction of clonogenic potential to the SP cell population as well as resurgence of nonSP cells from purified SP cell progenitors, while mixed culture assays using a GFP-vector construct tracing the SP vs. nonSP-population revealed homeostasis between the two populations, showing both SP and nonSP cells contributing to either cell compartment. SP cells show enhanced canonical wnt-signaling and increased exosomal secretion of wnt3a. Suppression of canonical wnt-signaling resulted in reduced clonogenicity. Exosome stimulation of DLBCL cell lines resulted in increased clonogenicity, stabilization of beta catenin and enhanced TOP/FOP activity. Conclusion Here we show that tumor cells reversibly switch between states of autonomous and non-autonomous clonogenicity, and that such transitions are regulated by exosome-mediated wnt signaling. Disclosures: No relevant conflicts of interest to declare.
30
Gallego-Martin, Teresa, Silvia Fernandez-Martinez, Ricardo Rigual, Ana Obeso, and Constancio Gonzalez. "Effects of low glucose on carotid body chemoreceptor cell activity studied in cultures of intact organs and in dissociated cells." American Journal of Physiology-Cell Physiology 302, no. 8 (April 15, 2012): C1128—C1140. http://dx.doi.org/10.1152/ajpcell.00196.2011.
Анотація:
The participation of the carotid body (CB) in glucose homeostasis and evidence obtained in simplified cultured CB slices or dissociated cells have led to the proposal that CB chemoreceptor cells are glucoreceptors. However, data generated in intact, freshly excised organs deny CB chemoreceptor cells' glucosensing properties. The physiological significance of the contention has prompted the present study, performed in a newly developed preparation of the intact CB organ in culture that maintains chemoreceptor cells' microenvironment. Chemoreceptor cells of intact CBs in culture retained their capacity to store, synthesize, and secrete catecholamine in response to hypoxia for at least 6 days. Aglycemia did not elicit neurosecretion in dissociated chemoreceptor cells or in intact CB in culture, but potentiated hypoxia-elicited neurosecretion, exclusively, in 1-day-old intact CB cultures and dissociated chemoreceptor cells cultured for 24 h. In fura 2-loaded cells, aglycemia (but not 1 mM) caused a slow Ca2+-dependent and nifedipine-insensitive increase in fluorescence at 340- to 380-nm wavelength emission ratio and augmented the fluorescent signal elicited by hypoxia. Association of nifedipine and KBR7943 (a Na+/Ca2+ exchanger inhibitor) completely abolished the aglycemic Ca2+ response. We conclude that chemoreceptor cells are not sensitive to hypoglycemia. We hypothesize that cultured chemoreceptor cells become transiently more dependent on glycolysis. Consequently, aglycemia would partially inhibit the Na+/K+ pump, causing an increase in intracellular Na+ concentration, and a reversal of Na+/Ca2+ exchanger. This would slowly increase intracellular Ca2+ concentration and cause the potentiation of the hypoxic responses. We discuss the nature of the signals detected by chemoreceptor cells for the CB to achieve its glycemic homeostatic role.
31
Ito, Atsushi, Takemasa Takii, Takayuki Matsumura, and Kikuo Onozaki. "Augmentation of Type I IL-1 Receptor Expression and IL-1 Signaling by IL-6 and Glucocorticoid in Murine Hepatocytes." Journal of Immunology 162, no. 7 (April 1, 1999): 4260–65. http://dx.doi.org/10.4049/jimmunol.162.7.4260.
Анотація:
Abstract IL-1 signal is transduced through type I receptor (IL-1RI). We have recently reported that LPS augments IL-1RI mRNA expression in the hepatocytes of mice in vivo, and the augmentation is mediated by the interaction of IL-1, IL-6, and glucocorticoid (GC). In this study, we examined whether IL-1RI mRNA expression level in the hepatocytes reflects those of cell surface molecule and IL-1 signaling. When primary cultured murine hepatocytes were treated with dexamethasone (Dex) or IL-6, these two reagents synergistically up-regulated IL-1RI mRNA expression in the cells. 125I-labeled IL-1 binding experiment showed that the level of binding was also up-regulated by the treatment with Dex and IL-6. Scatchard analysis revealed that the number of IL-1R increased. The increased binding of IL-1 was completely inhibited by an Ab against murine IL-1RI, indicating that Dex and IL-6 augmented the expression of cell surface IL-1RI molecule. When hepatocytes were pretreated with Dex and IL-6, the activation of IL-1R-associated kinase was augmented in response to IL-1, indicating that IL-1 signaling was also augmented. In addition, IL-1 treatment following administration of the combination of Dex and IL-6 into mice markedly increased the serum level of serum amyloid A. These results indicate that GC and IL-6 augment the expression of cell surface IL-1RI in hepatocytes, as well as IL-1 signaling and IL-1R-associated kinase activation, through up-regulation of IL-1RI mRNA level, which represents a novel regulatory network between IL-1, GC, and IL-6.
32
Itoh, K., K. Shiiba, Y. Shimizu, R. Suzuki, and K. Kumagai. "Generation of activated killer (AK) cells by recombinant interleukin 2 (rIL 2) in collaboration with interferon-gamma (IFN-gamma)." Journal of Immunology 134, no. 5 (May 1, 1985): 3124–29. http://dx.doi.org/10.4049/jimmunol.134.5.3124.
Анотація:
Abstract Activation of human peripheral blood mononuclear cells (PBMC) by interleukin 2 (IL 2) and the role of interferon-gamma (IFN-gamma) in the IL 2-induced activation were investigated. Activated killer (AK) cells against NK-resistant tumor cell lines were induced in the medium containing recombinant IL 2 (rIL 2) and autologous serum without any other stimulating agents. AK activity was induced by doses of rIL 2 as low as 3 U/ml, and reached a maximum at 10(3) U/ml. Incubation of PBMC with rIL 2 resulted in IFN-gamma production and augmented NK activity after 1 day of culture, and in induction of AK cells and proliferative response after 2 days of culture. These results suggested that endogenous IFN-gamma was required for rIL 2-induction of AK cells and proliferative response. To prove this, PBMC were cultured with rIL 2 and rIFN-gamma or were pretreated with rIFN-gamma before culture with rIL 2. Both rIFN-gamma treatments of PBMC augmented rIL 2-induced AK activity and proliferative response. rIL 2-induced IFN-gamma production was also enhanced by the rIFN-gamma pretreatment of PBMC. The addition of anti-IFN-gamma antibody to rIL 2 cultures abrogated the rIL 2-induced NK augmentation, AK generation, and proliferative response in proportion to the decreased amounts of endogenous IFN-gamma detectable in culture. rIFN-gamma and/or rIL 2 cultures of PBMC increased Tac antigen expression on cell surfaces as measured by flow cytometry. Enhanced Tac expression by rIL 2 was abrogated by adding anti-IFN-gamma antibody. These data indicate that: 1) AK generation and IFN-gamma production are mediated by IL 2, and 2) IFN-gamma production may be required for IL 2 induction of AK cells and proliferative response. These finding are consistent with the hypothesis that AK generation involves a collaboration between IL 2 and IFN-gamma, in which IL 2 stimulates PBMC to produce IFN-gamma, which in turn acts as a differentiation signal that may be involved in the IL 2-initiated AK generation and proliferative response.
33
Kende, Judit, Karen Phalet, Wim Van den Noortgate, Aycan Kara, and Ronald Fischer. "Equality Revisited: A Cultural Meta-Analysis of Intergroup Contact and Prejudice." Social Psychological and Personality Science 9, no. 8 (September 12, 2017): 887–95. http://dx.doi.org/10.1177/1948550617728993.
Анотація:
Across cultures, intergroup contact—interpersonal interaction with out-group members—is associated with less prejudice. Contact research was criticized, however, for bypassing intergroup inequality in the wider society. We propose a cultural psychology approach grounding people’s contact experiences in culturally afforded ways of relating to out-groups. Extending Allport’s equal-status hypothesis to the culture level, we hypothesized that the contact–prejudice association would be stronger in egalitarian cultures and weaker in more hierarchical cultures. To test this hypothesis, we revisited Pettigrew and Tropp’s influential meta-analysis and augmented it with culture-level measures of equality and hierarchy values. Our meta-analysis of intergroup contact and prejudice in 660 samples across 36 cultures suggested that egalitarianism was related to stronger contact–prejudice associations. Cultural hierarchy values and social dominance orientation corresponded with weaker contact–prejudice associations. Cultures of equality made a difference over and above equal status in the contact situation.
34
Chae, Dong-Sik, Sang Joon An, Seongho Han, and Sung-Whan Kim. "Synergistic Therapeutic Potential of Dual 3D Mesenchymal Stem Cell Therapy in an Ischemic Hind Limb Mouse Model." International Journal of Molecular Sciences 24, no. 19 (September 27, 2023): 14620. http://dx.doi.org/10.3390/ijms241914620.
Анотація:
Three-dimensional (3D) culture systems have been widely used to promote the viability and metabolic activity of mesenchymal stem cells (MSCs). The aim of this study was to explore the synergistic benefits of using dual 3D MSC culture systems to promote vascular regeneration and enhance therapeutic potential. We used various experimental assays, including dual 3D cultures of human adipose MSCs (hASCs), quantitative reverse transcription polymerase chain reaction (qRT-PCR), in vitro cell migration, Matrigel tube network formation, Matrigel plug assay, therapeutic assays using an ischemic hind limb mouse model, and immunohistochemical analysis. Our qRT-PCR results revealed that fibroblast growth factor 2 (FGF-2), granulocyte chemotactic protein-2 (GCP-2), and vascular endothelial growth factor-A (VEGF-A) were highly upregulated in conventional 3D-cultured hASCs (ASC-3D) than in two-dimensional (2D)-cultured hASCs. Hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1), and stromal-cell-derived factor-1 (SDF-1) showed higher expression levels in cytokine-cocktail-based, 3D-cultured hASCs (ASC-3Dc). A conditioned medium (CM) mixture of dual 3D ASCs (D-3D; ASC-3D + ASC-3Dc) resulted in higher migration and Matrigel tube formation than the CM of single 3D ASCs (S-3D; ASC-3D). Matrigel plugs containing D-3D contained more red blood cells than those containing S-3D. D-3D transplantation into ischemic mouse hind limbs prevented limb loss and augmented blood perfusion when compared to S-3D transplantation. Transplanted D-3D also revealed a high capillary density and angiogenic cytokine levels and transdifferentiated into endothelial-like cells in the hind limb muscle. These findings highlight the benefits of using the dual 3D culture system to optimize stem-cell-based therapeutic strategies, thereby advancing the therapeutic strategy for ischemic vascular disease and tissue regeneration.
35
Sonoda, E., R. Matsumoto, Y. Hitoshi, T. Ishii, M. Sugimoto, S. Araki, A. Tominaga, N. Yamaguchi, and K. Takatsu. "Transforming growth factor beta induces IgA production and acts additively with interleukin 5 for IgA production." Journal of Experimental Medicine 170, no. 4 (October 1, 1989): 1415–20. http://dx.doi.org/10.1084/jem.170.4.1415.
Анотація:
Effects of transforming growth factor beta (TGF-beta) on IgA production by LPS-stimulated B cells have been studied. TGF-beta itself could augment polyclonal IgA production in concomitant inhibition of polyclonal IgM and IgG1 production. Furthermore, TGF-beta and IL-5 additively augmented IgA production. TGF-beta exerted its activity early in the culture (by 2 d in a 5-d culture) and IL-5 was required late in the culture. Surface IgA- (sIgA-) B cells responded to TGF-beta for the development of IgA-secreting cells. By contrast, sIgA+ B cells, but not sIgA- B cells, responded to IL-5 for IgA production. These results suggest that TGF-beta has a differential role in the induction of IgA production from IL-5 on murine-activated B cells.
36
Zawalich, Walter S., Marc Bonnet-Eymard, Kathleen C. Zawalich та Gordon C. Yaney. "Chronic exposure to TPA depletes PKCα and augments Ca-dependent insulin secretion from cultured rat islets". American Journal of Physiology-Cell Physiology 274, № 5 (1 травня 1998): C1388—C1396. http://dx.doi.org/10.1152/ajpcell.1998.274.5.c1388.
Анотація:
The insulin secretory responses of rat islets to glucose (15 mM), 12- O-tetradecanoylphorbol 13-acetate (TPA; 500 nM), and potassium (30 mM) were determined from perifused islets cultured for 22–24 h in CMRL-1066 medium (control cultured) or islets cultured in the additional presence of 500 nM TPA. Islet content of protein kinase C α (PKCα) and serine and threonine phosphoprotein patterns were also monitored after the culture period. Compared with freshly isolated islets, culturing alone had no adverse effect on the capacity of TPA or 30 mM potassium to stimulate secretion or on the islet content of PKCα. In agreement with previous studies, culturing in TPA reduced the islet content of immunoreactive PKCα by >95% and abolished the capacity of the phorbol ester to stimulate secretion during a subsequent dynamic perifusion. Culturing in TPA slightly improved the insulin secretory response to 15 mM glucose compared with control-cultured islets; however, sustained rates of 15 mM glucose-induced secretion from these islets were significantly less than the responses of freshly isolated islets. Islets cultured in TPA responded to 30 mM potassium with a markedly amplified insulin secretory response that was abolished by nitrendipine. Enhanced phosphorylation of several islet proteins was also observed in TPA-cultured islets compared with control-cultured islets. These findings demonstrate that culturing alone impairs glucose-induced secretion, a response that is improved but still subnormal compared with freshly isolated islet responses, if TPA is included in the culture medium. Sustained phosphorylation of several islet proteins in TPA-cultured islets may account, at least in part, for augmented calcium-dependent secretion.
37
Kirkwood, R. N., P. A. Thacker, and K. Rajkumar. "Effects of growth hormone and triiodothyronine on insulin-induced progesterone production by granulosa cells from prepubertal gilts." Canadian Journal of Animal Science 72, no. 3 (September 1, 1992): 589–93. http://dx.doi.org/10.4141/cjas92-070.
Анотація:
Two experiments were performed using granulosa cells from medium-sized follicles (2–4 mm) derived from prepubertal gilts. Cells were cultured in a serum-free medium at a density of either 1 or 2 × 106 viable cells per well (experiments 1 and 2, respectively). For exp. 1, porcine growth hormone (pGH) (0 or 100 ng mL−1) was included in the culture medium from the time of plating, and low-density lipoprotein (LDL) (100 μg mL−1) was added at 72 h. For exp. 2, granulosa cells were plated in a culture medium containing either pGH (0 or 100 ng mL−1) or triiodothyronine (T3) (0 or 5 ng mL−1) or both pGH T3; LDL was not included. For both experiments, after 24 h of culture, bovine insulin at 0, 10, 100 or 1000 ng mL−1 was included in the medium. Hormones were replaced at 48 and 72 h, and the cultures were terminated at 96 h. Results from exp. 1 indicated that insulin increased (P < 0.01) progesterone production in a dose-dependent manner, both in the presence and absence of LDL. This response was augmented (P < 0.01) by co-culture with pGH. Results from exp. 2 confirmed the augmenting effect of pGH (P < 0.01). It was further observed that T3 increased (P < 0.01) progesterone production when cultured with insulin at 1000 ng mL−1, but at lower insulin-inclusion levels, results were equivocal. The progesterone production response was greatest (P < 0.01) when cells were cultured with both pGH and T3 at insulin levels of 100 or 1000 ng mL−1. There appeared to be little relationship between the media concentrations of insulin-like growth factor 1 and progesterone. The present results suggest that relatively high levels of pGH and T3 will enhance the in vitro steroidogenic capabilities of porcine granulosa cells. Key words: Granulosa cells, GH, T3, insulin
38
Kato, M. "Growth hormone-releasing hormone augments voltage-gated Na+ current in cultured rat pituitary cells." American Journal of Physiology-Cell Physiology 270, no. 1 (January 1, 1996): C125—C130. http://dx.doi.org/10.1152/ajpcell.1996.270.1.c125.
Анотація:
The effect of human growth hormone-releasing hormone (hGHRH), a potent stimulator of adenylate cyclase in somatotrophs on the voltage-gated sodium current was determined by perforated patch clamp of cultured rat somatotrophs The amplitude of the voltage-gated sodium current was augmented by 65.3 +/- 20.6% (mean +/- SE, n = 7) by 10 nM hGHRH. This augmentation was reversibly blocked by 10 microM H-89 a specific inhibitor for adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase. The membrane-permeant analogue of cAMP, dibutyryl cAMP (5 mM), also augmented the voltage-gated sodium current by 39.6 +/- 7.4% (n = 10). There were no effects of hGHRH or dibutyryl cAMP on steady-state inactivation of the sodium current. In contrast, in the whole cell configuration of patch clamp, no augmentation of the sodium current was observed by hGHRH or by the membrane-permeant analogue of cAMP. These results suggest that hGHRH augments the peak amplitude of the voltage-gated sodium current in rat somatotrophs via phosphorylation by cAMP-dependent protein kinase. For this augmentation, the intracellular environment must be kept relatively intact.
39
Boscia, Roseanne, Jonas T. Johnson, Kangnian Chen, and Theresa L. Whiteside. "Evaluation of Therapeutic Potential of Interleukin 2- Expanded Tumor-Infiltrating Lymphocytes in Squamous Cell Carcinoma of the Head and Neck." Annals of Otology, Rhinology & Laryngology 97, no. 4 (July 1988): 414–21. http://dx.doi.org/10.1177/000348948809700416.
Анотація:
Tumor-infiltrating lymphocytes (TILs) were isolated from surgically excised squamous cell carcinoma of the head and neck. Immunohistology showed that the tumor was infiltrated by T-lymphocytes, many of which were activated as judged by the expression of the following surface antigens: HLA-DR, transferrin receptor, and receptors for interleukin 2 (IL2). Fresh TILs were unable to lyse natural killer cell (NK)-resistant and NK-sensitive tumor cell targets in chromium-release cytotoxicity assays. Tumor-infiltrating lymphocytes as well as autologous peripheral blood lymphocytes were cultured in media containing natural IL-2. Tumor-infiltrating lymphocytes proliferated better than autologous peripheral blood lymphocytes and exhibited sustained growth and antitumor effector function in long-term cultures. The IL2-expanded culture of TILs contained mostly CD8 + (suppressor/cytotoxic) T-lymphocytes with the morphology characteristic of lymphokine-activated killer cells. The ability to expand TILs with augmented antitumor activity in long-term cultures indicates that these cells could be therapeutically useful. Potential application of human TILs to adoptive immunotherapy of squamous cell carcinoma of the head and neck is discussed, and recent advances in immunotherapy with IL2-activated TILs are reviewed.
40
Palmer-Crocker, R. L., and J. S. Pober. "IL-4 induction of VCAM-1 on endothelial cells involves activation of a protein tyrosine kinase." Journal of Immunology 154, no. 6 (March 15, 1995): 2838–45. http://dx.doi.org/10.4049/jimmunol.154.6.2838.
Анотація:
Abstract IL-4 triggers tyrosine phosphorylation of a single major substrate (M(r) 145,000) in cultured human endothelial cells (EC) as detected by Western blot of whole cell lysates or of anti-phosphotyrosine immunoprecipitates. Phosphorylation of this substrate depends on IL-4 concentration (appearance at 10 U/ml, maximal at 300 to 1000 U/ml) and time of treatment (onset by 1 min, peak at 5 to30 min, duration of 60 to 120 min). Immunoprecipitation with specific mAb identified the phosphorylated substrate as the IL-4R. Treatment of EC with IL-4 alone causes only a small increase in the expression of vascular cell adhesion molecule-1 (VCAM-1), but IL-4 significantly augments the level of VCAM-1 expression induced by PMA. Pretreatment of EC with herbimycin A (0.5 to 1.0 microgram/ml) for 12 to 18 h abrogates both IL-4-induced tyrosine phosphorylation and IL-4-augmented VCAM-1 expression. This concentration of herbimycin A does not inhibit and may augment PMA-induced VCAM-1 expression in replicate wells. These observations suggest that IL-4 induction of VCAM-1 in EC involves the activation of an as yet unidentified protein tyrosine kinase that phosphorylates the IL-4R.
41
Appiah-Kubi, Abena O., Lionel Blanc, Sharon A. Singh, Sebastien Didier, Sehba Dsilva, Kyle W. H. Chan, Jeffrey Michael Lipton, and Johnson M. Liu. "Pomalidomide Augments Fetal Hemoglobin Production In Primary Erythroid Cells By a Novel Mechanism Modulating BCL11A But Not KLF-1." Blood 122, no. 21 (November 15, 2013): 314. http://dx.doi.org/10.1182/blood.v122.21.314.314.
Анотація:
Abstract Fetal hemoglobin (HbF) is a known modifier of sickle cell disease (SCD) severity. KLF-1 is a regulator of the globin switch. It does so by increasing beta-globin production and up-regulating BCL11A, a repressor of HbF synthesis. Pomalidomide, a second generation immunomodulatory drug (IMiD), regulates HbF and F-cell production during erythropoiesis in human CD34+ cells. The mechanism by which pomalidomide enhances F-cell production is not well understood. In this study, CD34+ cells were obtained after purification of peripheral blood and positive selection and cultured using a three-phase in vitro liquid culture system which recapitulates erythropoiesis, including terminal differentiation and enucleation, in the presence of no drug, pomalidomide, hydroxyurea, or dimethyl sulfoxide (DMSO; vehicle control). Erythroid differentiation was assessed morphologically and by flow cytometry using the transferrin receptor and glycophorin A as markers of erythroid maturation. Flow cytometry was used to quantify F-cells. RT-qPCR was used to quantify mRNA expression of BCL11A, KLF-1, and gamma-globin. Western blot was used to measure the total expression levels of BCL11A. In this culture system pomalidomide increased F-cells more than hydroxyurea in both SCD and normal control erythroid cultures. There was a significant decrease in BCL11A expression levels, a repressor of HbF synthesis, with pomalidomide but not with hydroxyurea. This decrease was seen in both SCD and normal samples. KLF-1 was not affected by pomalidomide. These findings suggest a very different mechanism of action for pomalidomide versus hydroxyurea in increasing F-cell production. Pomalidomide appears to target the erythroid specific BCL11A but not the more pleiotropic transcription regulator KLF-1. Since the F-cell production was augmented in the presence of pomalidomide in controls as well as SCD erythroid cultures this study suggests a role for pomalidomide in the pharmacologic augmentation of fetal hemoglobin levels, perhaps in addition to hydroxyurea, not only in SCD but in any beta-hemoglobinopathy. Disclosures: Chan: BioTheryX Inc: Employment.
42
Chen, W. F., and A. Zlotnik. "IL-10: a novel cytotoxic T cell differentiation factor." Journal of Immunology 147, no. 2 (July 15, 1991): 528–34. http://dx.doi.org/10.4049/jimmunol.147.2.528.
Анотація:
Abstract A previous report concluded that a new cytokine, designated IL-10, is a growth cofactor for thymocytes, spleen, and lymph node cells. In this report, we have focused on the effects of IL-10 on CD8+ spleen T cells. We first observed that IL-10 enhances the growth of CD8+ T cells to IL-2. We then investigated the effect of murine rIL-10 on the induction of murine effector CTL from CTL precursors (CTL-p) using both bulk and filler cell-free limiting-dilution cultures. IL-10 alone could not induce Con A-activated FACS-sorted CD8+ T cells either to proliferate or to generate effector CTL. In combination with IL-2, however, IL-10 augmented the cytolytic activity of effector CTL generated from Con A-activated spleen CD8+ T cells in bulk cultures incubated for 5 days. In limiting-dilution cultures (using solid-phase anti-CD3 mAb as stimulus), IL-10, in combination with IL-2, substantially increased the CTL-p frequency and augmented the cytolytic activity per clone expanded from one CD8+ T cell when compared with cells cultured in IL-2 alone. Kinetic studies showed that IL-10 is required at both early and late culture stages for optimal generation of effector CTL. The potentiating effects of IL-10 on CTL function were neutralized by an anti-IL-10 mAb. These results indicate that IL-10 has direct effects on mature T cells, and suggest that IL-10 also functions as a cytotoxic T cell differentiation factor, which promotes a higher number of IL-2-activated CTL-p to proliferate and differentiate into effector CTL. In contrast, IL-10 did not enhance significantly the lymphokine-activated killer cell activity of IL-2-grown CD8+ cytotoxic T cells.
43
Hauch, M., MV Gazzola, T. Small, C. Bordignon, L. Barnett, I. Cunningham, H. Castro- Malaspinia, RJ O'Reilly, and CA Keever. "Anti-leukemia potential of interleukin-2 activated natural killer cells after bone marrow transplantation for chronic myelogenous leukemia." Blood 75, no. 11 (June 1, 1990): 2250–62. http://dx.doi.org/10.1182/blood.v75.11.2250.2250.
Анотація:
Abstract The anti-leukemia potential of natural killer (NK) cells has been evaluated in 40 patients transplanted for chronic myelogenous leukemia (CML) to determine whether differences in NK cell function were correlated with subsequent leukemic relapse. Cells from patients and their donors were tested in 51Cr release assays against fully allogeneic CML targets and against cultured K562 targets; cells from 26 patients were tested against host-derived CML targets that were cryopreserved before transplantation. Cultured CML targets (K562) were highly susceptible to lysis by freshly isolated peripheral blood lymphocytes (PBL) and to a greater degree by PBL cultured in medium containing interleukin-2 (IL-2) in all assays performed. In contrast, noncultured CML targets were lysed only by IL-2-activated cells from a subset of patients. When present, lytic activity to CML targets was detectable as early as 3 weeks after bone marrow transplantation, and remained positive throughout the posttransplant period. Optimal lytic activity developed within the first week of culture and required greater than or equal to 250 U/mL of IL-2 in the culture medium. Lytic activity to fully allogeneic and host-derived CML targets appeared to be mediated by CD16+ and CD56+ cells but not by CD3+ cells. Lysis of allogeneic CML targets was variable, but patients could be divided into two groups: those with and those without lytic activity to the majority of targets tested. The basis for the differences in lytic activity could not be ascribed to target susceptibility to lysis, the proportion of NK cells in the cultures, or to the phenotype of the NK cell subsets in the cultures. When tested in parallel, the lytic activity of donor and recipient cultures against host-derived CML targets was highly correlated, suggesting that there may be inherent differences in the ability of NK cells to recognize CML targets. The risk of relapse for patients who failed to generate lytic activity against host-derived CML targets was significantly increased over that for patients with lytic activity against host leukemia. These data indicate that posttransplant immunotherapy with IL-2 designed to activate NK cells will likely augment the graft-versus-leukemia potential of the graft.
44
Hauch, M., MV Gazzola, T. Small, C. Bordignon, L. Barnett, I. Cunningham, H. Castro- Malaspinia, RJ O'Reilly, and CA Keever. "Anti-leukemia potential of interleukin-2 activated natural killer cells after bone marrow transplantation for chronic myelogenous leukemia." Blood 75, no. 11 (June 1, 1990): 2250–62. http://dx.doi.org/10.1182/blood.v75.11.2250.bloodjournal75112250.
Анотація:
The anti-leukemia potential of natural killer (NK) cells has been evaluated in 40 patients transplanted for chronic myelogenous leukemia (CML) to determine whether differences in NK cell function were correlated with subsequent leukemic relapse. Cells from patients and their donors were tested in 51Cr release assays against fully allogeneic CML targets and against cultured K562 targets; cells from 26 patients were tested against host-derived CML targets that were cryopreserved before transplantation. Cultured CML targets (K562) were highly susceptible to lysis by freshly isolated peripheral blood lymphocytes (PBL) and to a greater degree by PBL cultured in medium containing interleukin-2 (IL-2) in all assays performed. In contrast, noncultured CML targets were lysed only by IL-2-activated cells from a subset of patients. When present, lytic activity to CML targets was detectable as early as 3 weeks after bone marrow transplantation, and remained positive throughout the posttransplant period. Optimal lytic activity developed within the first week of culture and required greater than or equal to 250 U/mL of IL-2 in the culture medium. Lytic activity to fully allogeneic and host-derived CML targets appeared to be mediated by CD16+ and CD56+ cells but not by CD3+ cells. Lysis of allogeneic CML targets was variable, but patients could be divided into two groups: those with and those without lytic activity to the majority of targets tested. The basis for the differences in lytic activity could not be ascribed to target susceptibility to lysis, the proportion of NK cells in the cultures, or to the phenotype of the NK cell subsets in the cultures. When tested in parallel, the lytic activity of donor and recipient cultures against host-derived CML targets was highly correlated, suggesting that there may be inherent differences in the ability of NK cells to recognize CML targets. The risk of relapse for patients who failed to generate lytic activity against host-derived CML targets was significantly increased over that for patients with lytic activity against host leukemia. These data indicate that posttransplant immunotherapy with IL-2 designed to activate NK cells will likely augment the graft-versus-leukemia potential of the graft.
45
Solon, Marius, and Liliana Solon. "Model integrat al culturii arhitecturale București (MICAB)." Argument. Spațiul construit. Concept și expresie, no. 10 (2018): 207–17. http://dx.doi.org/10.54508/argument.10.11.
Анотація:
Globalization is a reality of contemporary society, which cannot be ignored being present in all domains of life wherever we are. It is not only an academic debate theme, but it also appears as a process of our existence interdependences, dominating however we relate to it, even if we reject or accept it. The national identity, which formed the ideological basis for the national states, is presently facing with the phenomenon of cultural globalization, which brings together national cultures, but also melts identities. Based on the principle of action-reaction, the globalization founds the reverse into the processes of regionalization, localization, organization of the economical-social life closer to the individual and his needs. The Bucharest integrated architectural culture model (BIACM) brings into the debate the content and the structure of information coming from various domains of culture and social sciences which could become part of the new augmented reality structures, actual form of media communication.
46
Kadir, Rais Reskiawan A., Mansour Alwjwaj, Zoe McCarthy та Ulvi Bayraktutan. "Therapeutic hypothermia augments the restorative effects of PKC-β and Nox2 inhibition on an in vitro model of human blood–brain barrier". Metabolic Brain Disease 36, № 7 (16 серпня 2021): 1817–32. http://dx.doi.org/10.1007/s11011-021-00810-8.
Анотація:
AbstractTo investigate whether therapeutic hypothermia augments the restorative impact of protein kinase C-β (PKC-β) and Nox2 inhibition on an in vitro model of human blood–brain barrier (BBB). Cells cultured in normoglycaemic (5.5 mM) or hyperglycaemic (25 mM, 6 to 120 h) conditions were treated with therapeutic hypothermia (35 °C) in the absence or presence of a PKC-β inhibitor (LY333531, 0.05 μM) or a Nox2 inhibitor (gp91ds-tat, 50 μM). BBB was established by co-culture of human brain microvascular endothelial cells (HBMECs) with astrocytes (HAs) and pericytes. BBB integrity and function were assessed via transendothelial electrical resistance (TEER) and paracellular flux of sodium fluorescein (NaF, 376 Da). Nox activity (lucigenin assay), superoxide anion production (cytochrome-C reduction assay), cellular proliferative capacity (wound scratch assay) and actin cytoskeletal formation (rhodamine-phalloidin staining) were assessed both in HBMECs and HAs using the specific methodologies indicated in brackets. Therapeutic hypothermia augmented the protective effects of PKC-β or Nox2 inhibition on BBB integrity and function in experimental setting of hyperglycaemia, as evidenced by increases in TEER and concomitant decreases in paracellular flux of NaF. The combinatory approaches were more effective in repairing physical damage exerted on HBMEC and HA monolayers by wound scratch and in decreasing Nox activity and superoxide anion production compared to sole treatment regimen with either agent. Similarly, the combinatory approaches were more effective in suppressing actin stress fibre formation and maintaining normal cytoskeletal structure. Therapeutic hypothermia augments the cerebral barrier-restorative capacity of agents specifically targeting PKC-β or Nox2 pathways.
47
Gorodeski, George I. "Calcium regulates estrogen increase in permeability of cultured CaSki epithelium by eNOS-dependent mechanism." American Journal of Physiology-Cell Physiology 279, no. 5 (November 1, 2000): C1495—C1505. http://dx.doi.org/10.1152/ajpcell.2000.279.5.c1495.
Анотація:
Estrogen increases baseline transepithelial permeability across CaSki cultures and augments the increase in permeability in response to hypertonic gradients. In estrogen-treated cells, lowering cytosolic calcium abrogated the hypertonicity-induced augmented increase in permeability and decreased baseline permeability to a greater degree than in estrogen-deprived cells. Steady-state levels of cytosolic calcium in estrogen-deprived cells were higher than in estrogen-treated cells. Increases in extracellular calcium increased cytosolic calcium more in estrogen-deprived cells than in estrogen-treated cells. However, in estrogen-treated cells, increasing cytosolic calcium was associated with greater increases in permeability in response to hypertonic gradients than in estrogen-deprived cells. Lowering cytosolic calcium blocked the estrogen-induced increase in nitric oxide (NO) release and in the in vitro conversion of l-[3H]arginine to l-[3H]citrulline. Treatment with estrogen upregulated mRNA of the NO synthase isoform endothelial nitric oxide synthase (eNOS). These results indicate that cytosolic calcium mediates the responses to estrogen and suggest that the estrogen increase in permeability and the augmented increase in permeability in response to hypertonicity involve an increase in NO synthesis by upregulation of the calcium-dependent eNOS.
48
Heo, D. S., T. L. Whiteside, A. Kanbour, and R. B. Herberman. "Lymphocytes infiltrating human ovarian tumors. I. Role of Leu-19 (NKH1)-positive recombinant IL-2-activated cultures of lymphocytes infiltrating human ovarian tumors." Journal of Immunology 140, no. 11 (June 1, 1988): 4042–49. http://dx.doi.org/10.4049/jimmunol.140.11.4042.
Анотація:
Abstract Tumor-infiltrating lymphocytes (TIL) were obtained from human ovarian tumors, expanded in the presence of IL-2 in culture and studied for cytotoxicity against fresh autologous and allogeneic ovarian carcinoma (CA) targets. TIL from ovarian tumors grew well in long term cultures, achieving from 8- to 682-fold expansion. TIL cultured with IL-2 were cytotoxic against both autologous and allogeneic fresh ovarian CA targets, and no specificity for autologous tumor could be demonstrated in any of the cultures. In all fresh TIL preparations, CD3+ lymphocytes were the major cell type and contained a high proportion (up to 51%) of activated (IL-2R+) cells as determined by two-color flow cytometry. Sorting of bulk TIL cultures followed by cytotoxicity assays identified the Leu-19+ cells, both CD3+ and CD3-, as effectors of cytotoxicity against autologous and allogeneic tumor cell targets. Cold target inhibition assays showed that allogeneic targets (both ovarian CA and a sarcoma) competed effectively with autologous ovarian CA targets for Leu-19+ effectors in TIL cultures. mAb to Leu-19 or Leu-2a did not block lysis of autologous targets by sorted effectors. OKT3 antibody augmented lysis of autologous targets by CD3+Leu-19- effectors only. These results show that non-MHC-restricted Leu-19+ effectors in cultures of TIL with 1000 U/ml of rIL-2 mediate lysis of autologous and allogeneic tumor cells. The CD3+Leu-19- cells, the main population in these cultures, do not mediate tumor lysis. To determine the phenotype of antitumor effectors in IL-2 cultures of TIL, cell sorting followed by functional assays are necessary.
49
Herbert, Carol P. "Cultural Aspects of Dementia." Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques 28, S1 (May 2001): S77—S82. http://dx.doi.org/10.1017/s0317167100001244.
Анотація:
This paper critically reviews current knowledge regarding culture and dementia in order to identify unanswered questions in the field. Medline was searched from 1993-2000. One hundred nine articles were identified, of which 59 were critically reviewed, augmented by additional references from experts and by books. Limited research evidence was identified in four areas: 1) the recognition of dementia across cultures 2) cultural specificity of screening tools 3) identification of differences in risk factors, incidence, onset and prevalence across culture 4) culturally related issues in decision making about management. Implications for research and practice are described.
50
Kurniawan, Andreas. "Virtual Art Exhibition to Encourage Traditional Culture Knowledge for Generation-Z." E3S Web of Conferences 388 (2023): 04009. http://dx.doi.org/10.1051/e3sconf/202338804009.
Анотація:
Indonesia as a country that has a lot of traditional cultures, requires attention especially among generation-Z to maintain its sustainability. In many cases, various Indonesian cultures are often claimed by other countries as part of their culture, sometimes for the political purposes, economic, or even industrial motives. Thus, it is important to introduce and strengthen the traditional cultures knowledge, especially to generation-Z as the nation's successors. This study was conducted by creating a virtual gallery exhibition as a manifestation of new media forms that can adapt to the trends from generation-Z. The virtual gallery contains traditional attractions, culinary, dance, tourism, music, folklores, and ethnic houses. Some contents in this virtual gallery applies augmented reality technology with image tracking based. This research used mix method with qualitative and quantitative approach to explore the generation-Z expression. Results, virtual gallery gives an effective penetration to increase the traditional culture knowledge among generation- Z.