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Добірка наукової літератури з теми "Cucumber mosaic virus Genetics"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 28 січня 2023

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Статті в журналах з теми "Cucumber mosaic virus Genetics"

Avgelis, A. "Cucumber Mosaic Virus on Banana in Crete." Journal of Phytopathology 120, no. 1 (September 1987): 20–24. http://dx.doi.org/10.1111/j.1439-0434.1987.tb04410.x.

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Ragozzino, A., and D. Stefanis. "Urospermum picroides ospite naturale del virus del mosaico del cetriolo (Cucumber mosaic virus) e del virus del mosaico dell'erba medica (Alfalfa mosaic virus)1)." Journal of Phytopathology 86, no. 1 (June 28, 2008): 27–36. http://dx.doi.org/10.1111/j.1439-0434.1976.tb04654.x.

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Listihani, Listihani, Dewa Gede Wiryangga Selangga, and Mimi Sutrawati. "NATURAL INFECTION OF Tobacco mosaic virus ON BUTTERNUT SQUASH IN BALI, INDONESIA." JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA 21, no. 2 (July 18, 2021): 116–22. http://dx.doi.org/10.23960/jhptt.221116-122.

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Natural infection of Tobacco mosaic virus on butternut squash in Bali, Indonesia. Tobacco mosaic virus (TMV) was a newly emerging virus infecting cucumbers in Indonesia since 2017. The mosaic disease caused by TMV potentially caused yield loss cucumber in Java. In 2019, mosaic symptoms were observed in butternut squash plants in Bali and molecular detection using universal primer of Tobamovirus indicated the presence of TMV infection. Further research was conducted to determine molecular characteristics of TMV on butternut squash plants in Bali. Specific DNA bands of Tobamovirus were amplified using reverse transcription polymerase chain reaction method, followed by DNA sequencing. The DNA were successfully amplified from CP Tobamovirus using universal primers from several butternut squash samples, i.e. Denpasar, Gianyar, Buleleng, and Karangasem Districts. The homology analysis of nucleotide and amino acid sequences of TMV among isolates from Denpasar, Gianyar, Buleleng, and Karangasem Districts was ranged between 95.6 – 97.7% and 98.1 – 99.4%, respectively. This indicated that low genetic diversity of TMV among Bali isolates. The highest homology of corresponding sequences of TMV isolates from Denpasar, Gianyar, Buleleng, and Karangasem Districts was closely related to TMV Kediri-Indonesia isolate on cucumber plant. Correspondingly, the phylogenetic analysis showed that TMV Bali isolates were categorized into same cluster with Kediri-Indonesia isolates. This was the first report of TMV on butternut squash in Indonesia.

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Petrovic, Dragana, Maja Ignjatov, Zorica Nikolic, Milka Vujakovic, Mirjana Vasic, Mirjana Milosevic, and Ksenija Taski-Ajdukovic. "Occurrence and distribution of viruses infecting the bean in Serbia." Archives of Biological Sciences 62, no. 3 (2010): 595–601. http://dx.doi.org/10.2298/abs1003595p.

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This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia. .

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Walters, S. Alan. "Influence of Watermelon Mosaic Virus on Slicing Cucumber Farmgate Revenues." HortTechnology 14, no. 1 (January 2004): 144–48. http://dx.doi.org/10.21273/horttech.14.1.0144.

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Watermelon mosaic virus (WMV) is often the most limiting factor to cucumber (Cucumis sativus) production in the midwestern U.S. The influence of WMV on farm-gate revenues for nine slicing cucumber (or fresh market cucumber) cultivars was determined under high WMV disease incidence during 2000 and 2001. Over the two growing seasons, most cucumber cultivars produced excessive amounts of unmarketable WMV symptomatic fruit; however, no WMV symptoms were observed on any fruit produced by `Daytona' or `Indy'. `Thunder' produced some WMV symptomatic fruit but was significantly (P ≤ 0.05) less than that produced by all other cucumber cultivars, except for `Daytona' and `Indy.' Consistent high total farm gate-revenues over both years were produced by `Daytona' and `Indy' compared to other cucumber cultivars evaluated with the exception of `Thunder'. `Daytona,' `Indy,' and `Thunder' tended to produce greater early-season farm-gate revenues. However, late-season revenues of `Thunder' were reduced compared to `Daytona' and `Indy'. `Dasher II,' `General Lee,' `Greensleeves,' `Marketmore 76,' `Speedway,' and `Turbo' produced excessive amounts of unmarketable WMV symptomatic fruit which led to reduced farm-gate revenues. Cucumber cultivars without some level of resistance to WMV produced substantially less cumulative farm-gate revenues than those that had some level of resistance. `Daytona,' `Indy,' and `Thunder' were not the highest yielding cucumber cultivars evaluated in this study, but produced the highest farm-gate revenues due to higher levels of genetic resistance to WMV.

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Fisher, J. R., and S. G. P. Nameth. "Characterization of a Cucumber Mosaic Virus Isolate and Satellite RNA from the Ornamental Host Ajuga reptans `Royalty'." Journal of the American Society for Horticultural Science 128, no. 2 (March 2003): 231–37. http://dx.doi.org/10.21273/jashs.128.2.0231.

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Cucumber mosaic virus (CMV) was isolated from the perennial ornamental mint, Ajuga reptans L. `Royalty', using melon aphids (Aphis gossypii Glover). The isolate and its associated satellite RNA (satRNA) were biologically and chemically characterized. The satRNA was cloned and sequenced and is 338 nucleotides long and does not induce lethal necrosis on `Rutgers' tomato (Lycopersicon esculentum Mill.) or severe chlorosis on tobacco (Nicotiana L. spp.). The virus is ≈28 to 30 nm in diameter and reacts to CMV serological subgroup I antibodies. The virus is able to infect `Black Beauty' squash (Cucurbita pepo L.), cucumber (Cucumis sativus L.), and `Howden' pumpkin (Cucurbita pepo) but is not able to infect green bean (Phaseolus vulgaris L.) or cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]. The virus is able to efficiently replicate its satRNA in tobacco and `Black Beauty' squash but replication is less efficient in cucumber, based on accumulation of double-stranded satRNA.

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Walkey, D. G. A., C. M. Ward, and K. Phelps. "The reaction of lettuce (Lactuca sativa L.) cultivars to cucumber mosaic virus." Journal of Agricultural Science 105, no. 2 (October 1985): 291–97. http://dx.doi.org/10.1017/s0021859600056367.

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SUMMARYAll 53 lettuce cultivars inoculated with cucumber mosaic virus became infected. Leaf mosaic symptoms were generally mild and unreliable for distinguishing degrees of resistance between cultivars. Yield reduction was the most satisfactory criterion for evaluating resistance, with reductions in individual cultivars ranging from 8 to 50%.

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Kim, Shin Je, Kyung-Hee Paek, and Byung-Dong Kim. "Delay of Disease Development in Transgenic Petunia Plants Expressing Cucumber Mosaic Virus I17N-Satellite RNA." Journal of the American Society for Horticultural Science 120, no. 2 (March 1995): 353–59. http://dx.doi.org/10.21273/jashs.120.2.353.

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A cDNA clone of cucumber mosaic virus (CMV) 117 N-satellite RNA driven by the cauliflower mosaic virus (CaMV) 35S transcript promoter, was stably integrated into the genome of Petunia hybrida `Bluepicoti' tissues by Agrobacterium tumefaciens Ti plasmid-mediated transformation. Transgenic plants producing CMV satellite RNA showed delayed disease development when inoculated with CMV-Y, a helper virus for the I17N-satellite RNA. Furthermore, transgenic petunia plants showed delayed disease development against tobacco mosaic virus (TMV), a tobamovirus not related to CMV. Northern blot analysis revealed that large amounts of unit length satellite RNA (335 bp) were produced in CMV-infected transgenic petunia plants; whereas, mainly transcripts driven by the CaMV 35S promoter (approximately 1 kb) were produced in TMV-infected transgenic plants. SDS-PAGE and Western blotting showed that symptom reduction was correlated with a reduction in the amount of viral coat protein in transgenic plants.

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Davino, S., S. Cugnata, and M. G. Bellardi. "Globularia nudicaulis, a new host for Cucumber mosaic virus." Plant Pathology 55, no. 4 (August 2006): 568. http://dx.doi.org/10.1111/j.1365-3059.2006.01422.x.

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GERA, A., and J. COHEN. "Occurrence of cucumber mosaic virus in phlox in Israel." Plant Pathology 39, no. 3 (September 1990): 558–60. http://dx.doi.org/10.1111/j.1365-3059.1990.tb02533.x.

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Дисертації з теми "Cucumber mosaic virus Genetics"

Wahyuni, Wiwiek Sri. "Variation among cucumber mosaic virus (CMV) isolates and their interaction with plants." Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phw137.pdf.

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Includes appendix containing journal publications co-authored by the author. Includes bibliographical references (leaves 130-151). Eighteen strains of Cucumber mosaic virus, including forteen from Australia, two from the USA, and two from Japan were used in this study.

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Afsharifar, Alireza. "Characterisation of minor RNAs associated with plants infected with cucumber mosaic virus." Title page, table of contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09pha2584.pdf.

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Bibliography: leaves 127-138. This thesis studies the minor double stranded RNAs (dsRNA) and single stranded RNAs (ssRNA) which are consistently associated with plants infected with Q strain of cucumber mosaic virus (Q-CMV). The investigations are focused on the structural elucidation of new RNAs which have been observed in single stranded and double stranded RNA profiles of Q strain of CMV.

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Williams, Rhys Harold Verdon George. "Further studies on the structure and function of the cucumber mosaic virus genome : a thesis submitted to the University of Adelaide, South Australia for the degree of Doctor of Philosophy." 1988, 1988. http://web4.library.adelaide.edu.au/theses/09PH/09phw7261.pdf.

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Chen, Baoshan. "Encapsidation of nucleic acids by cucumovirus coat proteins /." Title page, contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09PH/09phc5183.pdf.

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Yang, Rongchang. "Towards genetic engineering cucumber mosaic virus (CMV) resistance in lupins." Thesis, Yang, Rongchang ORCID: 0000-0003-2563-2015 (2000) Towards genetic engineering cucumber mosaic virus (CMV) resistance in lupins. PhD thesis, Murdoch University, 2000. https://researchrepository.murdoch.edu.au/id/eprint/41568/.

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Cucumber mosaic virus (CMV) is a serious pathogen of many economically important crops. In Western Australia (WA), CMV is a serious disease of narrow-leafed lupin, Lupinus angustifolius, which is the main grain legume crop. There is no known natural resistance genes to CMV have been identified .in narrow-leafed lupin germplasm that can be transferred to new cultivars using classical breeding techniques. The aim of this project was to develop a series of molecular resistance constructs and to apply them to produce pathogen-derived resistance to CMV in narrow-leafed lupin. A total of nine different CMV resistance gene constructs were developed. Eight constructs were based on the movement protein (MP), coat protein (CP) and replicase (Rep) genes of the WA subgroup II CMV-LY isolate originally obtained from infected narrow-leafed lupin, and one was based on the CP gene of a WA subgroup I CMV isolate from banana. The gene constructs were cloned into the plant binary vectors pYR2 and pART27/7 driven by promoters from subterranean clover stunt virus (pPLEX) and cauliflower mosaic virus (CaMV 35S) and transferred into Agrobacterium (strain: AGL0). The constructs were used to transform Nicotiana benthamiana and narrow-leafed lupin with Basta as the selectable agent. For N. benthamiana a total of 1,120 explants were cocultivated with A. tumefaciens containing the pART27/7 resistance gene constructs (80 explants per construct). Following selection in culture, 16 putative transformants for each construct were transferred to the glasshouse for seed production and analysis. PCR analysis of T1 plants indicated that transformation had been successfully achieved for each of the resistance gene constructs. Transgenic plants were challenged with CMV and susceptibility or resistance was analysed by symptom development and ELISA. The results showed that some transgenic N. benthamiana plants that contained the Repm1 gene (defective CMV-LY ORF2a) were resistant to CMV-LY. In twenty-two PCR positive T₁ plants, 7 showed immunity, 12 were partially resistant, and 3 were susceptible to CMV-LY infection. In contrast, the antisense defective CMV-LY RNA 2 construct (Repm2) did not give good resistance to CMV-LY. Three of 12 T₁ plants with this construct were partially resistant (or had delayed symptoms) and the other nine were susceptible. Transgenic T1 plants containing a CMV-LY MP sense gene (MP1) showed limited resistance to CMV-LY. Two of 12 plants showed partial resistance (delayed symptoms) and two exhibited a recovery phenotype. The development of disease symptoms in the susceptible plants was faster than that in other transgenic and nontransgenic plants. Plants with the MPS transgene (untranslatable CMV-LY MP gene) showed some resistance to CMV-LY. One of 11 plants was highly resistant and three were partially resistant to CMV-LY. Three different versions of CMV-LY CP gene (CPI, CP3 and CP4) were transformed into N. benthamiana and the T₁ plants were challenged with CMV-LY. The level of resistance varied in transgenic plants depending on the CP genes present. Although a limited number of transgenic plants have been tested so far, it appears that plants containing CP4-1 show more effective resistance to CMV than transgenic plants with either CPl-1 or CP3-1. This result appears to be the first example of the use of a mutated CP gene that is longer than the wild type gene product (12 additional amino acids) and confers resistance to CMV. For narrow-leafed lupin, 12,411 explants were subjected to meristem inoculation and cocultivated with A. tumefaciens containing a replicase construct (pYRRepm1) and 3,134 explants with a movement protein construct (pYRMPS1). One hundred and sixty one independent transformants survived in vitro selection and were grafted onto compatible nontransgenic rootstocks. Fifty nine plants survived the grafting process and were transferred to the glasshouse for seed production. PCR analysis of the 59 putative transgenic lines (T0) identified 7 plants positive for the pYRRepml gene and 15 for the p YRMPS 1 gene. The complete genomic sequence of the CMV-LY isolate was also determined. The RNA1 molecule was determined to be 3,391 nucleotides (nt) in length and is predicted to contain a 5' untranslated region (UTR) of 95 nucleotides, a single open-reading frame (ORF) of 992 amino acids and a 3' UTR of 317 nt. The RNA2 molecule is 3,038 nt long and is predicted to contain a 5' UTR of 92 nt, two ORFs of 841 and 100 amino acids (ORF2a and ORF2b, respectively) and a 3' UTR of 423 nt. The RNA3 molecule is 2,003 nt long and is predicted to contain a 5' UTR of 96 nt, two ORFs of 279 and 218 amino acids (ORF3a and ORF3b, respectively) and a 3' UTR of 322 nt. Nucleotide comparisons of RNAs1-3 indicate that the LY isolate shares between 70-78% and 98-99% homology to subgroup I and subgroup II isolates, respectively. Similarly, ORFla shares 84-85% and 99% identity, ORF2a 81-84% and 94-96% identity, ORF2b 46-56% and 95-96% identity, ORF3a 82-84% and 99% identity, and ORF3b 81-83% and 99% identity. The sequence data clearly shows that there is a high degree of nucleotide and amino acid sequence homology between the CMV-LY isolate and other CMV subgroup II strains. The sequence data confirms that the LY isolate belongs to CMV subgroup II.

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Geering, Andrew D. W. "The epidemiology of cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius) in South Australia." Title page, table of contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phg298.pdf.

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Balcı, Evrim Doğanlar Sami. "Genetic characterization of cucumber mosaic virus(CMV)resistance in tomato and pepper." [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/biyoloji/T000388.pdf.

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Tamisier, Lucie. "Adaptation des populations virales aux résistances variétales et exploitation des ressources génétiques des plantes pour contrôler cette adaptation." Thesis, Avignon, 2017. http://www.theses.fr/2017AVIG0696/document.

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L’utilisation de variétés de plantes porteuses de gènes majeurs de résistance a longtemps été une solution privilégiée pour lutter contre les maladies des plantes. Cependant, la capacité des agents pathogènes à s’adapter à ces variétés après seulement quelques années de culture rend nécessaire la recherche de résistances à la fois efficaces et durables. Les objectifs de cette thèse étaient (i) d’identifier chez la plante des régions génomiques contraignant l’évolution des agents pathogènes en induisant des effets de dérive génétique et (ii) d’étudier l’impact des forces évolutives induites par la plante sur la capacité d’adaptation des pathogènes aux résistances variétales, l’ambition étant par la suite d’employer au mieux ces forces pour limiter l’évolution des pathogènes. Le pathosystème piment (Capsicum annuum) – PVY (Potato virus Y) a été principalement utilisé pour mener ces travaux de recherche. Afin de répondre au premier objectif, une cartographie de QTL (quantitative trait loci) sur une population biparentale de piment et une étude de génétique d’association sur une core-collection de piments ont été réalisées. Ces deux approches ont permis de mettre en évidence des régions génomiques sur les chromosomes 6, 7 et 12 impliquées dans le contrôle de la taille efficace des populations virales lors de l’étape d’inoculation du virus dans la plante. Certains de ces QTL ont montré une action vis-à-vis du PVY et du CMV (Cucumber mosaic virus) tandis que d’autres se sont révélés être spécifiques d’une seule espèce virale. Par ailleurs,le QTL détecté sur le chromosome 6 co-localise avec un QTL précédemment identifié comme contrôlant l’accumulation virale et interagissant avec un QTL affectant la fréquence de contournement d’un gène majeur de résistance. Pour répondre au second objectif, une analyse de la corrélation entre l’intensité des forces évolutives induites par la plante et une estimation expérimentale de la durabilité du gène majeur a été réalisée. De l’évolution expérimentale de populations de PVY sur des plantes induisant des effets de dérive génétique, de sélection et d’accumulation virale contrastés a également été effectuée. Ces deux études ont démontré qu’une plante induisant une forte dérive génétique associée à une réduction de l’accumulation virale permettait de contraindre l’évolution des populations virales, voire d’entraîner leur extinction. Ces résultats ouvrent de nouvelles perspectives pour le déploiement de déterminants génétiques de la plante qui influenceraient directement le potentiel évolutif du pathogène et permettraient de préserver la durabilité des gènes majeurs de résistance
Plants carrying major resistance genes have been widely used to fight against diseases. However, the pathogensability to overcome the resistance after a few years of usage requires the search for efficient and durable resistances.The objectives of this thesis were (i) to identify plant genomic regions limiting pathogen evolution by inducinggenetic drift effects and (ii) to study the impact of the evolutionary forces imposed by the plant on the pathogenability to adapt to resistance, the goal being to further use these forces to limit pathogen evolution. The pepper(Capsicum annuum) – PVY (Potato virus Y) pathosystem has been mainly used to conduct these researches.Regarding the first objective, quantitative trait loci (QTL) were mapped on a biparental pepper population andthrough genome-wide association on a pepper core-collection. These approaches have allowed the detection ofgenomic regions on chromosomes 6, 7 and 12 controlling viral effective population size during the inoculationstep. Some of these QTLs were common to PVY and CMV (Cucumber mosaic virus) while other were virusspecific.Moreover, the QTL detected on chromosome 6 colocalizes with a previously identified QTL controllingPVY accumulation and interacting with a QTL affecting the breakdown frequency of a major resistance gene.Regarding the second objective, a correlation analysis between the evolutionary forces imposed by the plant andan experimental estimation of the durability of a major resistance gene has been done. Experimental evolution ofPVY populations on plants contrasted for the levels of genetic drift, selection and virus accumulation they imposedhas also been performed. Both studies demonstrated that a plant inducing a strong genetic drift combined to areduction in virus accumulation limits virus evolution and could even lead to the extinction of the virus population.These results open new perspectives to deploy plant genetic factors directly controlling pathogen evolutionarypotential and could help to preserve the durability of major resistance genes

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McQuillin, Andrew. "Aspects of cucumber mosaic virus replication." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321682.

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Tungadi, Trisna Dewi. "Cucumber mosaic virus modifies plant-aphid interactions." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708288.

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Книги з теми "Cucumber mosaic virus Genetics"

Boulton, Margaret I. Protein synthesis in cucumber mosaic virus infected cucumber protoplasts. Birmingham: University of Birmingham, 1985.

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Wallington, Emma Jane. Studies on transgenic resistance to cucumber mosaic virus. Birmingham: University of Birmingham, 1992.

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Weiland, John J. The roles of turnip yellow mosaic virus genes in virus replication. 1992.

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Tsai, Ching-Hsiu. Characterization of the role of the 3' noncoding region of turnip yellow mosaic virus RNA. 1993.

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Wallace, S. Ellen. Search for protein-protein interactions underlying the cis-preferential replication of turnip yellow mosaic virus. 1997.

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Wallace, S. Ellen. Search for protein-protein interactions underlying the cis-preferential replication of turnip yellow mosaic virus. 1997.

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Bransom, Kathryn L. Gene expression of proteins involved in replication of turnip yellow mosaic virus. 1994.

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Частини книг з теми "Cucumber mosaic virus Genetics"

Shintaku, Michael, and Peter Palukaitis. "Genetic Mapping of Cucumber Mosaic Virus." In Viral Genes and Plant Pathogenesis, 156–64. New York, NY: Springer New York, 1990. http://dx.doi.org/10.1007/978-1-4612-3424-1_16.

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García-Arenal, Fernando, José Luis Alonso-Prados, Miguel A. Aranda, José M. Malpica, and Aurora Fraile. "Mixed Infections and Genetic Exchange Occur in Natural Populations of Cucumber Mosaic Cucumovirus." In Virus-Resistant Transgenic Plants: Potential Ecological Impact, 94–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-662-03506-1_11.

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Okada, Yoshimi. "Cucumber Green Mottle Mosaic Virus." In The Plant Viruses, 267–81. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-7026-0_14.

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Georgieva, I. D., and E. Stoimenova. "Cytochemical Investigation of Tomato and Cucumber Pollen After Cucumber Mosaic Virus Infection." In Progress in Botanical Research, 223–26. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5274-7_49.

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Tasaki, Keisuke, Masumi Yamagishi, and Chikara Masuta. "Virus-Induced Gene Silencing in Lilies Using Cucumber Mosaic Virus Vectors." In Methods in Molecular Biology, 1–13. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0751-0_1.

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Baulcombe, David, Martine Devic, and Martine Jaegle. "The Molecular Biology of Satellite RNA from Cucumber Mosaic Virus." In Recognition and Response in Plant-Virus Interactions, 263–72. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74164-7_13.

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García-Arenal, F., and P. Palukaitis. "Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus." In Current Topics in Microbiology and Immunology, 37–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-662-09796-0_3.

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Piazzolla, P., A. M. Tamburro, and V. Renugopalakrishnan. "Structural studies of cucumber mosaic virus: Fourier transform infrared spectroscopic studies." In Proteins, 133–37. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-010-9063-6_19.

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Chen, Jishuang. "Gene Cloning of Cucumber Mosaic Virus and Some Related Viral Agents." In Advanced Topics in Science and Technology in China, 1–46. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14119-5_1.

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Westwood, Jack H., and John P. Carr. "Cucumber Mosaic Virus-ArabidopsisInteraction: Interplay of Virulence Strategies and Plant Responses." In Molecular Plant Immunity, 225–50. Oxford, UK: Wiley-Blackwell, 2012. http://dx.doi.org/10.1002/9781118481431.ch11.

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Тези доповідей конференцій з теми "Cucumber mosaic virus Genetics"

"Reactivation of VaSTS1 expression in transgenic Arabidopsis thaliana plants by retransformation with 2b from Cucumber mosaic virus, isolate NK." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-125.

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"Reactivation of VaSTS1 expression in Arabidopsis thaliana transgenic plants by retransformation with 2b from the Cucumber Mosaic Virus isolate NK." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-45.

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Shehu, Dhurata, Thanas Ruci, Mirjana Stanoevska, and Lefteri Onuzi. "IDENTIFICATION of Cucumber mosaic virus (CMV) ON KUKES DISTRICT, ALBANIA." In The 4th Global Virtual Conference. Publishing Society, 2016. http://dx.doi.org/10.18638/gv.2016.4.1.755.

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Nasir, Aya Ali, and Mustafa Adhab. "A Biologically Distinct Isolate of Cucumber mosaic virus from Iraq." In 2021 Third International Sustainability and Resilience Conference: Climate Change. IEEE, 2021. http://dx.doi.org/10.1109/ieeeconf53624.2021.9668142.

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Chandra, Mukesh, Pallavi Somvanshi, B. N. Mishra, and Amod Tiwari. "Genetics of Yellow Mosaic Virus Resistance in Mung bean." In 2010 IEEE International Conference on Computational Intelligence and Computing Research (ICCIC). IEEE, 2010. http://dx.doi.org/10.1109/iccic.2010.5705760.

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Plotnikov, Kirill, Valeriya Ryabinina, Alevtina Khodakova, and Natalia Blazhko. "Viral Load Distribution of Cucumber Green Mottle Mosaic Virus in Leaves." In Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200113.171.

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Molad, Ori, Elisheva Smith, Neta Luria, Noa Sela, Oded Lachman, Elena Bakelman, Diana Leibman, and Aviv Dombrovsky. "Plant Disease Symptomatology: Cucumber Green Mottle Mosaic Virus (CGMMV)-Infected Cucumber Plants Exposed to Fluctuating Extreme Temperatures." In IECPS 2021. Basel Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/iecps2021-11991.

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Lee, Hoonsoo, Hyoun-Sub Lim, and Byoung-Kwan Cho. "Classification of cucumber green mottle mosaic virus (CGMMV) infected watermelon seeds using Raman spectroscopy." In SPIE Commercial + Scientific Sensing and Imaging, edited by Moon S. Kim, Kuanglin Chao, and Bryan A. Chin. SPIE, 2016. http://dx.doi.org/10.1117/12.2228264.

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Ryabinina, Valeriya, Sergey Pashkovsky, Kirill Plotnikov, and Eugeniya Gordienko. "Dynamics of Cucumber Green Mottle Mosaic Virus Accumulation and its Association to the Disease Manifestation." In Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200113.141.

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Uda, M. N. A., C. M. Hasfalina, A. A. Samsuzana, S. Faridah, Rafidah A. R., U. Hashim, Shahrul A. B. Ariffin, and Subash C. B. Gopinath. "Determination of set potential voltages for cucumber mosaic virus detection using screen printed carbon electrode." In 11TH ASIAN CONFERENCE ON CHEMICAL SENSORS: (ACCS2015). Author(s), 2017. http://dx.doi.org/10.1063/1.4975289.

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Звіти організацій з теми "Cucumber mosaic virus Genetics"

Gera, Abed, Abed Watad, P. Ueng, Hei-Ti Hsu, Kathryn Kamo, Peter Ueng, and A. Lipsky. Genetic Transformation of Flowering Bulb Crops for Virus Resistance. United States Department of Agriculture, January 2001. http://dx.doi.org/10.32747/2001.7575293.bard.

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Objectives. The major aim of the proposed research was to establish an efficient and reproducible genetic transformation system for Easter lily and gladiolus using either biolistics or Agrobacterium. Transgenic plants containing pathogen-derived genes for virus resistance were to be developed and then tested for virus resistance. The proposal was originally aimed at studying cucumber mosaic virus (CMV) resistance in plants, but studies later included bean yellow mosaic virus (BYMV). Monoclonal antibodies were to be tested to determine their effectiveness in interning with virus infection and vector (aphid) transmission. Those antibodies that effectively interfered with virus infection and transmission were to be cloned as single chain fragments and used for developing transgenic plants with the potential to resist virus infection. Background to the topic. Many flower crops, as lily and gladiolus are propagated vegetatively through bulbs and corms, resulting in virus transmission to the next planting generation. Molecular genetics offers the opportunity of conferring transgene-mediated disease resistance to flower crops that cannot be achieved through classical breeding. CMV infects numerous plant species worldwide including both lilies and gladioli. Major conclusions, solutions and achievements. Results from these for future development of collaborative studies have demonstrated the potential transgenic floral bulb crops for virus resistance. In Israel, an efficient and reproducible genetic transformation system for Easter lily using biolistics was developed. Transient as well as solid expression of GUS reporter gene was demonstrated. Putative transgenic lily plantlets containing the disabled CMV replicase transgene have been developed. The in vitro ability of monoclonal antibodies (mAbs) against CMV to neutralize virus infectivity and block virus transmission by M. persicae were demonstrated. In the US, transgenic Gladiolus plants containing either the BYMV coat protein or antisense coat protein genes have been developed and some lines were found to be virus resistant. Long-term expression of the GUS reporter gene demonstrated that transgene silencing did not occur after three seasons of dormancy in the 28 transgenic Gladiolus plants tested. Selected monoclonal antibody lines have been isolated, cloned as single chain fragments and are being used in developing transgenic plants with CMV resistance. Ornamental crops are multi-million dollar industries in both Israel and the US. The increasing economic value of these floral crops and the increasing ban numerous pesticides makes it more important than ever that alternatives to chemical control of pathogens be studied to determine their possible role in the future. The cooperation resulted in the objectives being promoted at national and international meetings. The cooperation also enabled the technology transfer between the two labs, as well as access to instrumentation and specialization particular to the two labs.

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Gal-On, Amit, Shou-Wei Ding, Victor P. Gaba, and Harry S. Paris. role of RNA-dependent RNA polymerase 1 in plant virus defense. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597919.bard.

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Objectives: Our BARD proposal on the impact of RNA-dependent RNA polymerase 1 (RDR1) in plant defense against viruses was divided into four original objectives. 1. To examine whether a high level of dsRNA expression can stimulate RDR1 transcription independent of salicylic acid (SA) concentration. 2. To determine whether the high or low level of RDR1 transcript accumulation observed in virus resistant and susceptible cultivars is associated with viral resistance and susceptibility. 3. To define the biogenesis and function of RDR1-dependent endogenous siRNAs. 4. To understand why Cucumber mosaic virus (CMV) can overcome RDR1-dependent resistance. The objectives were slightly changed due to the unique finding that cucumber has four different RDR1 genes. Background to the topic: RDR1 is a key plant defense against viruses. RDR1 is induced by virus infection and produces viral and plant dsRNAs which are processed by DICERs to siRNAs. siRNAs guide specific viral and plant RNA cleavage or serve as primers for secondary amplification of viral-dsRNA by RDR. The proposal is based on our preliminary results that a. the association of siRNA and RDR1 accumulation with multiple virus resistance, and b. that virus infection induced the RDR1-dependent production of a new class of endogenous siRNAs. However, the precise mechanisms underlying RDR1 induction and siRNA biogenesis due to virus infection remain to be discovered in plants. Major conclusions, solutions and achievements: We found that in the cucurbit family (cucumber, melon, squash, watermelon) there are 3-4 RDR1 genes not documented in other plant families. This important finding required a change in the emphasis of our objectives. We characterized 4 RDR1s in cucumber and 3 in melon. We demonstrated that in cucumber RDR1b is apparently a new broad spectrum virus resistance gene, independent of SA. In melon RDR1b is truncated, and therefore is assumed to be the reason that melon is highly susceptible to many viruses. RDR1c is dramatically induced due to DNA and RNA virus infection, and inhibition of RDR1c expression led to increased virus accumulation which suggested its important on gene silencing/defense mechanism. We show that induction of antiviral RNAi in Arabidopsis is associated with production of a genetically distinct class of virus-activated siRNAs (vasiRNAs) by RNA dependent RNA polymerase-1 targeting hundreds of host genes for RNA silencing by Argonaute-2. Production of vasiRNAs is induced by viruses from two different super groups of RNA virus families, targeted for inhibition by CMV, and correlated with virus resistance independently of viral siRNAs. We propose that antiviral RNAi activate broad-spectrum antiviral activity via widespread silencing of host genes directed by vasiRNAs, in addition to specific antiviral defense Implications both scientific and agricultural: The RDR1b (resistance) gene can now be used as a transcription marker for broad virus resistance. The discovery of vasiRNAs expands the repertoire of siRNAs and suggests that the siRNA-processing activity of Dicer proteins may play a more important role in the regulation of plant and animal gene expression than is currently known. We assume that precise screening of the vasiRNA host targets will lead in the near future for identification of plant genes associate with virus diseases and perhaps other pathogens.

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Valverde, Rodrigo A., Aviv Dombrovsky, and Noa Sela. Interactions between Bell pepper endornavirus and acute viruses in bell pepper and effect to the host. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598166.bard.

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Based on the type of relationship with the host, plant viruses can be grouped as acute or persistent. Acute viruses are well studied and cause disease. In contrast, persistent viruses do not appear to affect the phenotype of the host. The genus Endornavirus contains persistent viruses that infect plants without causing visible symptoms. Infections by endornaviruses have been reported in many economically important crops, such as avocado, barley, common bean, melon, pepper, and rice. However, little is known about the effect they have on their plant hosts. The long term objective of the proposed project is to elucidate the nature of the symbiotic interaction between Bell pepper endornavirus (BPEV) and its host. The specific objectives include: a) to evaluate the phenotype and fruit yield of endornavirus-free and endornavirus-infected bell pepper near-isogenic lines under greenhouse conditions; b) to conduct gene expression studies using endornavirus-free and endornavirus-infected bell pepper near-isogenic lines; and c) to study the interactions between acute viruses, Cucumber mosaic virus Potato virus Y, Pepper yellow leaf curl virus, and Tobacco etch virus and Bell pepper endornavirus. It is likely that BPEV in bell pepper is in a mutualistic relationship with the plant and provide protection to unknown biotic or abiotic agents. Nevertheless, it is also possible that the endornavirus could interact synergistically with acute viruses and indirectly or directly cause harmful effects. In any case, the information that will be obtained with this investigation is relevant to BARD’s mission since it is related to the protection of plants against biotic stresses.

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Whitham, Steven A., Amit Gal-On, and Victor Gaba. Post-transcriptional Regulation of Host Genes Involved with Symptom Expression in Potyviral Infections. United States Department of Agriculture, June 2012. http://dx.doi.org/10.32747/2012.7593391.bard.

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Understanding how RNA viruses cause disease symptoms in their hosts is expected to provide information that can be exploited to enhance modern agriculture. The helper component-proteinase (HC-Pro) protein of potyviruses has been implicated in symptom development. Previously, we demonstrated that symptom expression is associated with binding of duplex small-interfering-RNA (duplex-siRNA) to a highly conserved FRNK amino acid motif in the HC-Pro of Zucchini yellow mosaic virus (ZYMV). This binding activity also alters host microRNA (miRNA) profiles. In Turnip mosaic virus (TuMV), which infects the model plant Arabidopsis, mutation of the FRNK motif to FINK was lethal providing further indication of the importance of this motif to HC-Pro function. In this continuation project, our goal was to further investigate how ZYMV and TuMV cause the mis-expression of genes in cucurbits and Arabidopsis, respectively, and to correlate altered gene expression with disease symptoms. Objective 1 was to examine the roles of aromatic and positively charged residues F164RNH and K215RLF adjacent to FR180NK in small RNA binding. Objective 2 was to determine the target genes of the miRNAs which change during HC-Pro expression in infected tissues and transgenic cucumber. Objective 3 was to characterize RNA silencing mechanisms underlying differential expression of host genes. Objective 4 was to analyze the function of miRNA target genes and differentially expressed genes in potyvirus-infected tissues. We found that the charged K/R amino acid residues in the FKNH and KRLF motifs are essential for virus viability. Replacement of K to I in FKNH disrupted duplex-siRNA binding and virus infectivity, while in KRLF mutants duplex-siRNA binding was maintained and virus infectivity was limited: symptomless following a recovery phenomenon. These findings expanded the duplex-siRNA binding activity of HC-Pro to include the adjacent FRNK and FRNH sites. ZYMV causes many squash miRNAs to hyper-accumulate such as miR166, miR390, mir168, and many others. Screening of mir target genes showed that only INCURVATA-4 and PHAVOLUTA were significantly upregulated following ZYMVFRNK infection. Supporting this finding, we found similar developmental symptoms in transgenic Arabidopsis overexpressing P1-HC-Pro of a range of potyviruses to those observed in miR166 mutants. We characterized increased transcription of AGO1 in response to infection with both ZYMV strains. Differences in viral siRNA profiles and accumulation between mild and severe virus infections were characterized by Illumina sequencing, probably due to the differences in HC-Pro binding activity. We determined that the TuMV FINK mutant could accumulate and cause symptoms in dcl2 dcl4 or dcl2 dcl3 dcl4 mutants similar to TuMV FRNK in wild type Arabidopsis plants. These dcl mutant plants are defective in antiviral defenses, and the results show that factors other than HC-ProFRNK motif can induce symptoms in virus-infected plants. As a result of this work, we have a better understanding of the FRNK and FKNH amino acid motifs of HC-Pro and their contributions to the duplex-siRNA binding functions. We have identified plant genes that potentially contribute to infectivity and symptoms of virus infected plants when they are mis-expressed during potyviral infections. The results establish that there are multiple underlying molecular mechanisms that lead viral pathogenicity, some dependent on HC-Pro. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.

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Grumet, Rebecca, and Benjamin Raccah. Identification of Potyviral Domains Controlling Systemic Infection, Host Range and Aphid Transmission. United States Department of Agriculture, July 2000. http://dx.doi.org/10.32747/2000.7695842.bard.

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Potyviruses form one of the largest and most economically important groups of plant viruses. Individual potyviruses and their isolates vary in symptom expression, host range, and ability to overcome host resistance genes. Understanding factors influencing these biological characteristics is of agricultural importance for epidemiology and deployment of resistance strategies. Cucurbit crops are subject to severe losses by several potyviruses including the highly aggressive and variable zucchini yellow mosaic virus (ZYMV). In this project we sought to investigate protein domains in ZYMV that influence systemic infection and host range. Particular emphasis was on coat protein (CP), because of known functions in both cell to cell and long distance movement, and helper component-protease (HC-Pro), which has been implicated to play a role in symptom development and long distance movement. These two genes are also essential for aphid mediated transmission, and domains that influence disease development may also influence transmissibility. The objectives of the approved BARD project were to test roles of specific domains in the CP and HC-Pro by making sequence alterations or switches between different isolates and viruses, and testing for infectivity, host range, and aphid transmissibility. These objectives were largely achieved as described below. Finally, we also initiated new research to identify host factors interacting with potyviral proteins and demonstrated interaction between the ZYMV RNA dependent RNA polymerase and host poly-(A)-binding protein (Wang et al., in press). The focus of the CP studies (MSU) was to investigate the role of the highly variable amino terminus (NT) in host range determination and systemic infection. Hybrid ZYMV infectious clones were produced by substituting the CP-NT of ZYMV with either the CP-NT from watermelon mosaic virus (overlapping, but broader host range) or tobacco etch virus (TEV) (non- overlapping host range) (Grumet et al., 2000; Ullah ct al., in prep). Although both hybrid viruses initially established systemic infection, indicating that even the non-cucurbit adapted TEV CP-NT could facilitate long distance transport in cucurbits, after approximately 4-6, the plants inoculated with the TEV-CPNT hybrid exhibited a distinct recovery of reduced symptoms, virus titer, and virus specific protection against secondary infection. These results suggest that the plant recognizes the presence of the TEV CP-NT, which has not been adapted to infection of cucurbits, and initiates defense responses. The CP-NT also appears to play a role in naturally occurring resistance conferred by the zym locus in the cucumber line 'Dina-1'. Patterns of virus accumulation indicated that expression of resistance is developmentally controlled and is due to a block in virus movement. Switches between the core and NT domains of ZYMV-NAA (does not cause veinal chlorosis on 'Dina-1'), and ZYMV-Ct (causes veinal chlorosis), indicated that the resistance response likely involves interaction with the CP-NT (Ullah and Grumet, submitted). At the Volcani Center the main thrust was to identify domains in the HC-Pro that affect symptom expression or aphid transmissibility. From the data reported in the first and second year report and in the attached publications (Peng et al. 1998; Kadouri et al. 1998; Raccah et al. 2000: it was shown that: 1. The mutation from PTK to PAK resulted in milder symptoms of the virus on squash, 2. Two mutations, PAK and ATK, resulted in total loss of helper activity, 3. It was established for the first time that the PTK domain is involved in binding of the HC-Pro to the potyvirus particle, and 4. Some of these experiments required greater amount of HC-Pro, therefore a simpler and more efficient purification method was developed based on Ni2+ resin.

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