Дисертації з теми "Crown-gall disease"
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Robbs, Steven Lynn 1961. "Genotypic variation in susceptibility of Pisum sativum to crown gall and characterization of one cultivar of pea with reduced susceptibility to crown gall." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277008.
Keegan, Alexandra Ruth. "Biological control of crown gall disease in Australian grapevine nurseries." Title page, contents and summary only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phk262.pdf.
Ahmadi, Ali-Reza. "The role of agrocin 434 and other factors in the biological control of crown gall disease /." Title page, table of contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09pha2858.pdf.
Chen, Hui, and 陳輝. "Effects of elicitors on the secondary metabolism of crown gall and hairy root cultures of salvia miltiorrhiza." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B3123995X.
Chen, Hui. "Effects of elicitors on the secondary metabolism of crown gall and hairy root cultures of salvia miltiorrhiza /." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22054911.
Fajardo, Norma N. "Biological and chemical aspects of agrocin 434 as a supplementary biocontrol agent for crown gall /." Title page, table of contents and summary only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phf175.pdf.
Copies of author's previously published articles inserted. Includes bibliographical references (leaves 72-90).
Donner, Scott Charles. "Agrocins from Agrobacteria /." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phd686.pdf.
Dombek, Priscilla Emily. "Functional domains of Agrobacterium tumefaciens single-stranded DNA binding protein VirE2." Thesis, 1996. http://hdl.handle.net/1957/34220.
Graduation date: 1997
Chen, Huan-Yu, and 陳奐宇. "Identification and characterization of the causal agent of roselle crown gall disease." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/8unany.
國立臺灣大學
植物醫學碩士學位學程
106
In Taiwan, the cultivation area of roselle is about 124.88 hectare. Taitung county is main region for production of roselle. Since 2016, a new crown gall disease was found in the commercial plantations of roselle (Hibiscus sabdariffa) throughout Taimali and Jinfong in Taitung County. In summer 2017, we found the disease spread out to the other areas South of Ziben in Taitung County, and the estimated disease incidence was about 0.6-10%. Through Koch’s postulate, it was proved that the bacterial strains isolated from crown galls formed on roselle are pathogenic to roselle. They can induce gall formation on the stems of roselle, from which the same bacterial strain can be re-isolated, indicating that it should be the causal agent. Colony morphology of the isolated bacterial strain is viscous and milky white in color on the tryptone soy agar (TSA). In a carrot slice bioassay, tumor formation could be observed on the cambial regions after inoculation of the isolated strains. The pathogenic bacteria isolated in this study are rod-shaped and have peritrichous flagella. By means of 16S rDNA sequence analysis, recA-specific primers and Biolog microbial identification system, they were identified as Agrobacterium radiobacter (Beijerinck and van Delden 1902) Conn 1942 (Synonyms: Agrobacterium tumefaciens and Rhizobium radiobacter) whose physiological and biochemical characterisitics are similar to Agrobacterium rubi. As this disease was not recorded in “List of Plant Diseases in Taiwan” and is a new disease of roselle, it was then named “roselle crown gall disease”. A. radiobacter can grow from 20 to 37 °C, and pH 6-7 is optimal for its growth. It can induce tumor formation by stem puncture inoculation or soil drenching as long as wounds are present. When stem puncture inoculation was used, the minimal infection dose is 104 cfu/mL. Furthermore, significantly bigger galls were formed on meristems when the inoculum was 109 cfu/mL. Disinfection of equipment with 75 % ethanol and 3 % NaOCl can decrease the possibility of infection through pruning. Furthermore, Pseudomonas protegens XH1-2a、Pantoea ananatis A63 and Bacillus amyloliquefaciens PMB01, as well as tetracycline, streptomycin+ tetracycline and mancozeb, can inhibit growth of A. radiobacter in a disk diffusion test. In a greenhouse assay, although tumors were still formed after application of P. protegens XH1-2a, smaller galls could be observed when plants were treated with P. protegens XH1-2a before or together with A. radiobacter. Application of tetracycline before pathogen inoculation could effectively suppress gall formation. Tumor was barely formed while roselle plants were inoculated with A. radiobacter mixed with tetracycline. A. radiobacter could weaken roselle plants. Although it would not kill roselle plants, A. radiobacter might affect growth and yield of roselle when the diseas is getting worse. In this study, the causing agent of roselle crown gall disease was successfully identified and characterized, which would help us further investigate its ecology and develop control strategies for prevention or treatment of roselle crown gall disease to reduce the economic loss.
Keegan, Alexandra Ruth. "Biological control of crown gall disease in Australian grapevine nurseries / Alexandra Keegan." Thesis, 2001. http://hdl.handle.net/2440/21717.
Includes bibliographical references (leaves 192-205).
v, 206 leaves : ill. (some col.) ; 30 cm.
Thesis (Ph.D.)--University of Adelaide, Dept. of Applied and Molecular Ecology, 2001
Shen, Chia-Hsing, and 沈嘉興. "Characteristics of Agrobacterium tumefaciens and Biological Control of Rose Crown Gall Disease in Taiwan." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/82329016002976215842.
國立中興大學
植物病理學系
88
During recent years, crown gall disease has been found on rose, Ficus spp. and aster in Taiwan. Thirty-nine bacterial strains including 37 strains isolated from the galls of rose collected in Changhua, Nantou, Yunlin and Taichung, one strain obtained from the galls of aster in Pingtung, and the Agrobacterium radiobacter K1206 strain from Australia were used in this study. All strains had been tested for their ability to induce crown galls on carrot slices, tomato seedlings and rose seedlings. These bacterial strains were also tested by PCR analysis with Agrobacterium tumefaciens specific primer VirD1. The results showed that 33 strains could induce galls. And all of these 33 strains except AA19 which was obtained from aster, could be amplified the specific DNA fragment in PCR analysis. Based on cultural, physiological and biochemical tests, bacterial strains from rose crown gall in Taiwan were separated into two clusters when subjected to numerical taxonomic analysis. The first cluster had the characteristics which were distinct from both biovar 1 and biovar 2, whereas the second cluster had the characteristics similar to biovar 1. Biolog GN System was also used in this study to identify bacterial strains from rose crown gall in Taiwan. Based on the pathogenicity test, physiological and biochemical testes and Biolog identification, the 32 bacterial strains from rose in Taiwan were identified as A. tumefaciens. The control of crown gall on rose cuttings by immersion in A. radiobacter K1026 bacterial suspension before planting was tested. The results showed highly significant disease control. Galls were absent or less on the roots of rose seedlings that had been dipped in a suspension of K1026, and planted in soil infested with A. tumefaciens. A. radiobacter K1026 also effectively protected the pruning wounds from infection by A. tumefaciens.
Ahmadi, Ali-Reza 1960. "The role of agrocin 434 and other factors in the biological control of crown gall disease." 1996. http://web4.library.adelaide.edu.au/theses/09PH/09pha2858.pdf.
Ahmadi, Ali-Reza 1960. "The role of agrocin 434 and other factors in the biological control of crown gall disease / Ali-Reza Ahmadi." Thesis, 1996. http://hdl.handle.net/2440/18789.
Bibliography: leaves 114-136.
xiv, 144, [19] leaves, [18] leaves of plates : ill. (some col.) ; 30 cm.
The study concentrates on: investigation of the biological properties and role of agrocin 434 and other factors in the bicontrol process of crown gall disease; isolation of the complete range of K84 derivatives carrying different combinations of plasmids pAgK84, pAgK434 and pAtK84b in the same host background; development of a rapid plant bioassay to assess the efficacy of biocontrol strains by leaf disc tumorigenesis assay; and characterization of agrocin 434 synthesis and immunity genes.
Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1997
Fajardo, Norma N. "Biological and chemical aspects of agrocin 434 as a supplementary biocontrol agent for crown gall / Norma N. Fajardo." Thesis, 1995. http://hdl.handle.net/2440/18626.
Bibliography: leaves 72-90.
vii, 101, [28] leaves, [12] leaves of plates : ill. ; 30 cm.
Crown gall is a plant cancer caused by pathogenic strains of Agrobacterium, a soil microorganism belonging to the family Rhizobiaceae. This thesis examines the possible role in the biological control process, of an antibiotic compound agrocin 434, produced by biocontrol strains, in addition to the well-characterized adenine nucleotide, agrocin 84.
Thesis (Ph.D.)--University of Adelaide, Depts. of Crop Protection and Plant Science, 1996
Wang, Li-Chi, and 王莉琪. "Effects of Crown Gall Disease on Physiological Characteristics and Citronellal and Citronellol Concentrations of Eucalyptus Citriodora Seedlings." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/54228527638648886889.
國立嘉義大學
森林暨自然資源研究所
94
The objective of this study was to investigate the effects of Agrobacterium tumefaciens on physiological characteristics, and concentrations of citronellal and citronellol of Eucalyptus citriodora seedlings. In this study, the differences of physiological characteristics, and essential oil contents of healthy and diseased seedlings of E. citriodora were analyzed. A. tumefaciens was isolated from crown gall tissues of E. citriodora seedlings. The A. tumefaciens isolates were then inoculated onto sterilized plantlets and calli of E. citriodora. The results revealed that the A. tumefaciens could attack E. citriodora seedlings and caused crown gall disease. The physiological characteristics of infected E. citriodora seedlings changed. The results showed that the non-infected seedlings had higher averaged total dry weight, leaf area, leaf water content, specific leaf area, and nitrogen content of roots. However, the infected seedlings had higher phosphorus, citronellal, and citronellol contents than the non-infected ones. Ultrastructural study with scanning electron microscope showed that there were significant differences among stomatal densities of infected and non-infected E. citriodora seedlings. In this study, sterilized seeds were aseptically cultured and inoculated with A. tumefaciens. The result showed that the in vitro plantlets developed crown gall after inoculation with A. tumefaciens. Further study revealed that the infected calli of E. citriodora had higher citronellal and citronellol contents. This study demonstrated that A. tumefaciens could stimulate the phosphorus, citronellal, and citronellol contents in tissues of E. citriodora.
Lu, Shu-Fen. "Isolation of putative pAgK84 transconjugants from commerical cherry and raspberry plants treated with Agrobacterium radiobacter strain K84." Thesis, 1994. http://hdl.handle.net/1957/35284.
Shim, Je-Seop. "Studies on the agrocin 84 plasmid of `Agrobacterium radiobacter`." 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phs5565.pdf.
Shim, Je-Seop. "Studies on the agrocin 84 plasmid of `Agrobacterium radiobacter` / by Je-Seop Shim." Thesis, 1987. http://hdl.handle.net/2440/18544.
Bibliography: leaves 145-154
vii, 164 leaves : ill ; 30 cm.
Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1988
Pitrak, Jennifer. "Silencing the Agrobacterium tumefaciens oncogenes." Thesis, 2005. http://hdl.handle.net/1957/29276.
Graduation date: 2006
Biggs, John 1966. "Ecology and biological control of Agrobacterium vitis, the grapevine crown gall pathogen." 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phb5923.pdf.
Biggs, John 1966. "Ecology and biological control of Agrobacterium vitis, the grapevine crown gall pathogen / John Biggs." Thesis, 1994. http://hdl.handle.net/2440/21570.