Готові списки джерел за темами / Corneal epithelial cells

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Добірка наукової літератури з теми "Corneal epithelial cells"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 21 лютого 2023

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Статті в журналах з теми "Corneal epithelial cells"

Langer, Marian G., C. V. Sundarraj, and Nirmala Sundarraj. "Corneal epithelial-specific cell surface antigen recognized by a monoclonal antibody." Development 94, no. 1 (June 1, 1986): 163–72. http://dx.doi.org/10.1242/dev.94.1.163.

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Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted specifically with corneal epithelial cells and not with epithelial cells of other tissues when tested by an indirect immunoperoxidase technique. Nonidet P-40 extracts of different subcellular fractions of human corneal epithelial cells were tested for their reactivity against this antibody using an enzyme-linked immunosorbent assay. The results indicated that the antigen recognized by this antibody is associated with the plasma membrane. This was further verified by immuno-electron-microscopic analysis using ferritin-conjugated anti-mouse IgG antibody. This antigen was not detectable in the corneal epithelial cells in primary cultures nor in the epithelial cells from early stages of developing cornea (12 to 18 weeks in utero) but was present in the epithelial cells in the corneas of an 8-month-old infant. Therefore, this surface-associated antigen identified in the present study is a developmentally regulated marker of human corneal epithelium.

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Alarcon, Irania, Lesley Kwan, Chong Yu, David J. Evans, and Suzanne M. J. Fleiszig. "Role of the Corneal Epithelial Basement Membrane in Ocular Defense against Pseudomonas aeruginosa." Infection and Immunity 77, no. 8 (June 8, 2009): 3264–71. http://dx.doi.org/10.1128/iai.00111-09.

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ABSTRACT Pseudomonas aeruginosa can invade corneal epithelial cells and translocates multilayered corneal epithelia in vitro, but it does not penetrate the intact corneal epithelium in vivo. In healthy corneas, the epithelium is separated from the underlying stroma by a basement membrane containing extracellular matrix proteins and pores smaller than bacteria. Here we used in vivo and in vitro models to investigate the potential of the basement membrane to defend against P. aeruginosa. Transmission electron microscopy of infected mouse corneas in vivo showed penetration of the stroma by P. aeruginosa only where the basement membrane was visibly disrupted by scratch injury, suggesting that the intact basement membrane prevented penetration. This hypothesis was explored using an in vitro Matrigel Transwell model to mimic the corneal basement membrane. P. aeruginosa translocation of multilayered corneal epithelia grown on Matrigel was ∼100-fold lower than that of cells grown without Matrigel (P < 0.005, t test). Matrigel did not increase transepithelial resistance. Matrigel-grown cells blocked translocation by a P. aeruginosa protease mutant. Without cells, Matrigel also reduced traversal of P. aeruginosa and the protease mutant. Fluorescence microscopy revealed a relative accumulation of bacteria at the superficial epithelium of cells grown on Matrigel at 3 h compared to cells grown on uncoated filters. By 5 h, bacteria accumulated beneath the cells, suggesting direct trapping by the Matrigel. These findings suggest that the basement membrane helps defend the cornea against infection via physical barrier effects and influences on the epithelium and that these roles could be compromised by P. aeruginosa proteases.

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Hazlett, L. D., and P. Mathieu. "Glycoconjugates on corneal epithelial surface: effect of neuraminidase treatment." Journal of Histochemistry & Cytochemistry 37, no. 8 (August 1989): 1215–24. http://dx.doi.org/10.1177/37.8.2754252.

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The purpose of this study was to develop a procedure to quantitatively examine corneal epithelial apical cell membrane-associated glycoconjugates. Saccharide moieties on young, mature, and aged corneal epithelial cells were detected and localized in corneas of immature and adult mice by using colloidal gold-labeled lectins and transmission electron microscopy (TEM). In general, dense binding to the corneal epithelial apical surface cell membranes with wheat germ agglutinin (WGA) was seen in the adult, whereas the immature cornea bound less WGA-gold. Neuraminidase digestion decreased binding of the conjugate on epithelial plasma membranes of young and mature cells in adult cornea. Lectin-gold binding was decreased in the immature cornea on mature and aged cells. WGA-gold binding after neuraminidase was elevated on young cells of immature and on aged cells of adult animals. No binding of peanut agglutinin (PNA) or horse gram agglutinin (DBA) to the corneal epithelial surface was seen in animals of either age. After neuraminidase digestion, PNA binding sites were exposed only on the adult corneal surface. These data suggest that a terminal trisaccharide sequence, sialic acid-galactose beta(1----3)-N-acetylgalactosamine, is present at the adult corneal surface but is absent or at undetectable levels at the corneal surface of the immature animal. These data may be of significance in light of the dissimilar pattern of P. aeruginosa recognition and binding to the immature vs adult corneal epithelium.

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Augustin, Danielle K., Susan R. Heimer, Connie Tam, Wing Y. Li, Jeff M. Le Due, David J. Evans, and Suzanne M. J. Fleiszig. "Role of Defensins in Corneal Epithelial Barrier Function againstPseudomonas aeruginosaTraversal." Infection and Immunity 79, no. 2 (November 29, 2010): 595–605. http://dx.doi.org/10.1128/iai.00854-10.

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ABSTRACTStudies have shown that epithelium-expressed antimicrobial peptides (AMPs), e.g., β-defensins, play a role in clearing bacteria from mouse corneas already infected withPseudomonas aeruginosa. Less is known about the role of AMPs in allowing the cornea to resist infection when healthy. We previously reported that contact lens exposure, a major cause ofP. aeruginosakeratitis, can inhibit the upregulation of human β-defensin 2 (hBD-2) by corneal epithelial cells in response toP. aeruginosaantigensin vitro. Here, we studied the role of AMPs in maintaining the corneal epithelial barrier toP. aeruginosapenetration using bothin vitro(human) andin vivo(mouse) experiments. Results showed that preexposing human corneal epithelial multilayers to bacterial antigens in a culture supernatant (known to upregulate AMP expression) reduced epithelial susceptibility toP. aeruginosatraversal up to 6-fold (P< 0.001). Accordingly, small interfering RNA (siRNA) knockdown of any one of four AMPs expressed by human epithelia promotedP. aeruginosatraversal by more than 3-fold (P< 0.001). The combination knockdown of AMPs further enhanced susceptibility to bacterial traversal by ∼8-fold (P< 0.001).In vivoexperiments showed that the loss of murine β-defensin 3 (mBD-3), a murine ortholog of hBD-2, enhanced corneal susceptibility toP. aeruginosa. The uninjured ocular surface of mBD-3−/−mice showed a reduced capacity to clearP. aeruginosa, and their corneal epithelia were more susceptible to bacterial colonization, even when inoculatedex vivoto exclude tear fluid effects. Together, thesein vitroandin vivodata show functional roles for AMPs in normal corneal epithelial cell barrier function againstP. aeruginosa.

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Zieske, J. D., and M. Wasson. "Regional variation in distribution of EGF receptor in developing and adult corneal epithelium." Journal of Cell Science 106, no. 1 (September 1, 1993): 145–52. http://dx.doi.org/10.1242/jcs.106.1.145.

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Epidermal growth factor receptor has been localized to the proliferative cell layers in a variety of stratified squamous epithelia. In the current study, the rat cornea was used as an experimental model to determine if epidermal growth factor receptor is concentrated in epithelial stem cells. Epidermal growth factor receptor was localized using immunofluorescence microscopy in adult and neonatal (1-day to 4-week) rat corneas. Antibody binding to epidermal growth factor receptor was present in basal cells across the adult cornea but was more intense in the limbal zone. In rats 1 day to 1 week of age, the corneal epithelium consisted of one or two layer of cells that were intensely labeled by anti-epidermal growth factor receptor. Following epithelial stratification, which occurred just prior to eyelid opening (approximately 12 days), expression of epidermal growth factor receptor was greatly reduced in central corneal epithelium and gained an adult pattern by 3 weeks of age. Expression of epidermal growth factor receptor was also examined by incubating 1 mm slices of adult corneas with 125I-epidermal growth factor (4 nM) for 90 minutes, followed by washing and autoradiography. Basal cells in the limbal zone contained 4.5-fold more silver grains per cell than did basal cells in the central cornea. These data suggest that cells with high potential for proliferation, i.e. limbal basal cells and all basal cells in developing rats, express high epidermal growth factor receptor levels. High levels of receptor may allow these cells to be rapidly stimulated by growth factors to undergo cell division during development and following wounding in adult corneas.(ABSTRACT TRUNCATED AT 250 WORDS)

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Park, Mijeong, Alexander Richardson, Elvis Pandzic, Erwin P. Lobo, J. Guy Lyons, and Nick Di Girolamo. "Peripheral (not central) corneal epithelia contribute to the closure of an annular debridement injury." Proceedings of the National Academy of Sciences 116, no. 52 (December 16, 2019): 26633–43. http://dx.doi.org/10.1073/pnas.1912260116.

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Corneal epithelia have limited self-renewal and therefore reparative capacity. They are continuously replaced by transient amplifying cells which spawn from stem cells and migrate from the periphery. Because this view has recently been challenged, our goal was to resolve the conflict by giving mice annular injuries in different locations within the corneolimbal epithelium, then spatiotemporally fate-mapping cell behavior during healing. Under these conditions, elevated proliferation was observed in the periphery but not the center, and wounds predominantly resolved by centripetally migrating limbal epithelia. After wound closure, the central corneal epithelium was completely replaced by K14+limbal-derived clones, an observation supported by high-resolution fluorescence imaging of genetically marked cells in organ-cultured corneas and via computational modeling. These results solidify the essential role of K14+limbal epithelial stem cells for wound healing and refute the notion that stem cells exist within the central cornea and that their progeny have the capacity to migrate centrifugally.

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Han, Sang Beom, Farah Nur Ilyana Mohd Ibrahim, Yu-Chi Liu, and Jodhbir S. Mehta. "Efficacy of Modified Amnion-Assisted Conjunctival Epithelial Redirection (ACER) for Partial Limbal Stem Cell Deficiency." Medicina 57, no. 4 (April 10, 2021): 369. http://dx.doi.org/10.3390/medicina57040369.

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Background and objectives: the aim of this study was to analyze the efficacy of a modified “amnion-assisted conjunctival epithelial redirection (ACER)” technique for the treatment of partial limbal stem cell deficiency (LSCD). Materials and methods: the medical records of three patients with partial LSCD who underwent corneal surface reconstruction with modified ACER following superficial keratectomy were retrospectively studied. Briefly, in this technique, an inner amniotic membrane (AM) layer was applied on the corneal surface to promote corneal re-epithelialization. The outer AM layer was applied as a barrier to prevent the invasion of conjunctival epithelial cells into the cornea before the corneal surface was completely covered by corneal epithelial cells derived from the remaining intact limbal stem cells. Results: in all three cases, the outer AM layer successfully kept the conjunctival epithelium away from the corneal surface and prevented an admixture of conjunctival epithelial cells with corneal epithelial cells. In all three patients, the cornea was completely re-epithelized with epithelial cells derived from the remaining healthy limbal stem cells, and a clear visual axis was maintained without recurrence for a mean follow-up period of 37.3 ± 8.6 months. Conclusions: the preliminary results suggest that modified ACER appears to be a viable option for patients with partial LSCD.

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Ryu, Jin Suk, So Yeon Kim, Mee Kum Kim, and Joo Youn Oh. "Inflammation Confers Healing Advantage to Corneal Epithelium following Subsequent Injury." International Journal of Molecular Sciences 24, no. 4 (February 7, 2023): 3329. http://dx.doi.org/10.3390/ijms24043329.

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Recent evidence shows that epithelial stem/progenitor cells in barrier tissues such as the skin, airways and intestines retain a memory of previous injuries, which enables tissues to accelerate barrier restoration after subsequent injuries. The corneal epithelium, the outermost layer of the cornea, is the frontline barrier for the eye and is maintained by epithelial stem/progenitor cells in the limbus. Herein, we provide evidence that inflammatory memory also exists in the cornea. In mice, eyes that had been exposed to corneal epithelial injury exhibited faster re-epithelialization of the cornea and lower levels of inflammatory cytokines following subsequent injury (either the same or a different type of injury) relative to naïve eyes without previous injury. In ocular Sjögren’s syndrome patients, corneal punctate epithelial erosions were significantly reduced after experiencing infectious injury compared with before. These results demonstrate that previous exposure of the corneal epithelium to inflammatory stimuli enhances corneal wound healing in response to a secondary assault, a phenomenon which points to the presence of nonspecific inflammatory memory in the cornea.

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Schermer, A., S. Galvin, and T. T. Sun. "Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells." Journal of Cell Biology 103, no. 1 (July 1, 1986): 49–62. http://dx.doi.org/10.1083/jcb.103.1.49.

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In this paper we present keratin expression data that lend strong support to a model of corneal epithelial maturation in which the stem cells are located in the limbus, the transitional zone between cornea and conjunctiva. Using a new monoclonal antibody, AE5, which is highly specific for a 64,000-mol-wt corneal keratin, designated RK3, we demonstrate that this keratin is localized in all cell layers of rabbit corneal epithelium, but only in the suprabasal layers of the limbal epithelium. Analysis of cultured corneal keratinocytes showed that they express sequentially three major keratin pairs. Early cultures consisting of a monolayer of "basal" cells express mainly the 50/58K keratins, exponentially growing cells synthesize additional 48/56K keratins, and postconfluent, heavily stratified cultures begin to express the 55/64K corneal keratins. Cell separation experiments showed that basal cells isolated from postconfluent cultures contain predominantly the 50/58K pair, whereas suprabasal cells contain additional 55/64K and 48/56K pairs. Basal cells of the older, postconfluent cultures, however, can become AE5 positive, indicating that suprabasal location is not a prerequisite for the expression of the 64K keratin. Taken together, these results suggest that the acidic 55K and basic 64K keratins represent markers for an advanced stage of corneal epithelial differentiation. The fact that epithelial basal cells of central cornea but not those of the limbus possess the 64K keratin therefore indicates that corneal basal cells are in a more differentiated state than limbal basal cells. These findings, coupled with the known centripetal migration of corneal epithelial cells, strongly suggest that corneal epithelial stem cells are located in the limbus, and that corneal basal cells correspond to "transient amplifying cells" in the scheme of "stem cells----transient amplifying cells----terminally differentiated cells."

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Bardag-Gorce, Fawzia, Alissa Diaz, Robert Niihara, Jeremy Stark, Daileen Cortez, Alexander Lee, Richard Hoft, and Yutaka Niihara. "Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency." International Journal of Molecular Sciences 23, no. 7 (April 5, 2022): 4032. http://dx.doi.org/10.3390/ijms23074032.

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Purpose: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface with LSCD. Methods: New Zealand white rabbit autologous oral mucosal epithelial cells were isolated from a buccal biopsy and cultured to be grafted back onto corneas of rabbit model of LSCD. Immunofluorescent staining and Western blot analysis were used to compare the expression of ALDH1A1 and ALDH1A3 in healthy, LSCD-diseased, CAOMECS treated corneas. Human oral mucosal and corneal epithelial cells (OMECS and CECs) were cultured and treated with retinoic acid (RA) to further investigate the expression of ALDHs. Results: In healthy corneas, ALDH1A1 and ALDH1A3 were markedly expressed in basal cells of corneal epithelium. In LSCD diseased corneas, ALDH1A1 and ALDH1A3 were markedly expressed in the conjunctivalized apical epithelial cells, the goblet cells, and the stroma. CAOMECS grafted corneas showed a decreased expression of ALDHs as compared to LSCD diseased corneas. Western blot analysis confirmed the up regulation of ALDH1A1 and ALDH1A3 expression in LSCD-diseased corneal epithelial cells. CAOMECS expressed low levels of ALDH1A1 and ALDH1A3, as compared to diseased CECs (D-CEC). When ALDH1A3 was up regulated by retinoic acid treatment in OMECS, Pax-6 expression was down regulated, suggesting a decrease in regenerative capacity when ALDH enzymes are up regulated. Conclusions: These findings report for the first time the up regulation of ALDH1A1 and ALDH1A3 in rabbit corneas with LSCD and document that CAOMECS grafting used to reconstruct corneal epithelium may reduce the expression levels of ALDH enzymes.

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Більше джерел

Дисертації з теми "Corneal epithelial cells"

Liu, Ke. "Role of second messengers in controlling growth patterns of corneal epithelial cells /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030718.102224/index.html.

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Thesis (Ph. D.)--University of Western Sydney, 2002.
"This thesis is submitted in fulfilment of the requirements of the degree of Doctor of Philosophy to the University of Western Sydney School of Biological Sciences."t.p. Includes bibliographical references (leaves 138-150).

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Binti, Kamarudin Taty Anna. "Differentiation of human pluripotent stem cells into corneal epithelial like cells." Thesis, University of Newcastle upon Tyne, 2018. http://hdl.handle.net/10443/4182.

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Cornea is the clear outermost protective layer of the eye which enables transmission of light onto the retina. The corneal epithelium is regenerated by limbal stem cells (LSCs), whose loss/dysfunction results in limbal stem cell deficiency (LSCD). Transplantations of ex vivo expanded autologous LSCs from patient's healthy eye onto the affected eye have provided a successful treatment for unilateral LSCD. This however is not applicable to patient with total bilateral LSCD, whose both eyes are affected. This thesis investigated the potential of human induced-pluripotent stem cell (hiPSCs) to differentiate into corneal epithelial-like cells as a source of autologous stem cell treatment for patients with total bilateral LSCD, and tested the engraftment of the differentiated cells in LSCD mouse model. Combined addition of bone morphogenetic protein 4 (BMP4), all trans-retinoic acid (RA) and epidermal growth factor (EGF) for the first nine days of differentiation followed by cell-replating on collagen-IV coated surfaces with a corneal-specific-epithelial cell media for an additional 11 days, resulted in step wise differentiation of human embryonic stem cells (hESCs) to corneal epithelial progenitors and mature corneal epithelial-like cells. Differences in the ability of hiPSCs lines to undergo differentiation to corneal epithelial-like cells were observed. These were dependent on the level of endogenous BMP signalling and could be restored via activation of this signalling pathway by a specific TGFβ inhibitor (SB431542). The hESC and hiPSCs-derived corneal epithelial cells were transplanted into a LSCD mouse model where they survived up to 14 days, but failed to provide long term engraftment and corneal surface regeneration. The findings showed a differential ability of hESCs and hiPSCs lines to generate corneal epithelial cells which is underlined by the endogenous BMP signalling pathway activity. However, the engraftment and functionality of the differentiated cells in the LSCD animal model has yet to be improved.

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Liu, Ke. "Role of second messengers in controlling growth patterns of corneal epithelial cells." Thesis, View thesis, 2002. http://handle.uws.edu.au:8081/1959.7/387.

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The purpose of this thesis was to investigate mechanisms contolling the growth of corneal epithelial cells, particularly the intracellular signals involved with stratification compared with cellular migration and maturation. Buttons of epithelium were cultured in different culture media. The explants were monitored microscopically for their growth patterns and finally fixed and examined for cytokeratin, vimentin and actin. Different growth patterns were observed in the different media, indicating that different signalling patterns must be operating in these cells depending upon the media in which they were grown. To investigate the intracellular pathways controlling the different growth patterns, the protein phosphorylation of different cultures was investigated. The two proteins, p57 and p30, are strongly suggested to be associated with stratification of the epithelial cells. The possible involvement of the common serine kinase, PKC, in controlling the growth pattern of corneal epithelial cells were also investigated. The results suggested that an intracellular pathway involving PKC promotes the maturation and spread of the cells but is not involved in their stratification. These experiments taken together indicate that the different aspects of corneal epithelia cell growth are tightly controlled and may occur quite independently. Specific protein expression appears to be important for stratification, and phosphorylation of proteins by PKC appears to be involved with the maturation of epithelial cells from basal cells. It also indicates that the mature cells are capable of producing the extracellular matrix protein fibronectin which appears to have an important role in causing the spread as distinct from the stratification of the corneal epithelial cells.

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Liu, Ke, University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "Role of second messengers in controlling growth patterns of corneal epithelial cells." THESIS_CSTE_SFH_Liu_K.xml, 2002. http://handle.uws.edu.au:8081/1959.7/387.

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Guo, Ying. "Actin contractility in corneal epithelial cells and regulation of the barrier integrity." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3229600.

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Анотація:

Thesis (Ph.D.)--Indiana University, School of Optometry, 2006.
"Title from dissertation home page (viewed July 11, 2007)." Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4360. Adviser: Sangly Srinivas.

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Terry, S. J. "Identification of regulators and effectors of RhoGTPase signalling in corneal epithelial cells." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1306879/.

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Анотація:

Epithelial cells adhere to each other and are connected via a series of junctions. Tight junctions (TJs) are a specific type of junction consisting of heteromeric protein complexes that are linked to the actin cytoskeleton and are important in regulating paracellular permeability and cell polarity. RhoGTPases are small molecular switch proteins that are important regulators of the cytoskeleton and modulators of gene expression. RhoGTPases have thus been identified as being major signalling components associated with TJs. However little is known about how RhoGTPases are regulated to control junction formation and gene expression in corneal epithelial cells. I used a siRNA screening approach combined with functional assays to identify components of RhoGTPase signalling that affect the assembly of junctions and gene expression in Human corneal epithelial cells (HCE). I identified and validated several candidates that regulate junction assembly. One of these candidates was p114RhoGEF, a novel TJ localised guanine nucleotide exchange factor (GEF) important for the assembly of functional TJs. p114RhoGEF is a widely expressed and I discovered its depletion effects junction formation and morphogenesis in three dimensional culture systems in different epithelial cell types. p114RhoGEF is required for activation of RhoA at cell-cell junctions and junctional actinomyosin activity, p114RhoGEF is present in a complex containing Myosin II-A, the RhoA effector Rock II and the junctional adaptor protein Cingulin; indicating p114RhoGEF is a component of a junction associated RhoA-signalling module. p114RhoGEF, thus regulates spatial activation of RhoA at cell-cell junctions and organisation of the junctional cytoskeleton. p114RhoGEF may also have a role in cell migration, as depletion in HCE cells, caused cells to migrate at a slower rate during wound healing assays. I have also started to explore the function of a putative p114RhoGEF ortholog, cg10188 in Drosophila melanogaster. Preliminary experiments have identified cg10188 to be important in larval development.

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Bhattacharya, Pradipta. "The corneal epithelium in health and disease." Thesis, Queensland University of Technology, 2022.

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Анотація:

The cornea is the anterior transparent layer of the eye, and understanding corneal epithelial morphology is valuable in monitoring ocular surface diseases. A meta-analysis showed that central corneal basal cell density and nerve parameters were reduced in diseases affecting the ocular surface including limbal stem cell deficiency. Using newly developed image analysis techniques it was observed that epithelial cell density was highest at the corneal nerve whorl region. A decrease in epithelial cell density was observed in eyes with conjunctival ultraviolet autofluorescence, i.e. with sunlight-induced UV damage.

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Barnard, Zeke. "Analyses of Cytokeratins and p63 Isoforms Expressed by Human Limbal Epithelial Cells." Thesis, Griffith University, 2005. http://hdl.handle.net/10072/367809.

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Анотація:

Cultivated autologous limbal epithelial grafts have shown promise as a valuable treatment to treat ocular surface injuries. The rationale for this therapy is that the limbus contains progenitor cells of the corneal epithelium and that when expanded and transplanted these cells help to restore a healthy epithelium. Nevertheless, this approach is still experimental and the optimal procedure for producing, grafting and assessing the presence of limbal stem cells in these grafts remains under development. The first results chapter examines the differences in cytokeratin expression between donor corneoslceral rims and cultivated limbal epithelial cultures derived from these donor rims. The expression of keratins 3 and 14 is more stable between the in situ and in vitro environments, while keratins 6 and 19 appear to be upregulated in limbal epithelial cells in response to their isolation and culture. This highlights the role of keratins in limbal epithelial cell function and provides valuable knowledge for assessing the phenotype of cultivated limbal epithelial grafts. Cytokeratin expression is more important in identifying the transient amplifying population than the progenitor population, indicating the need for more specific markers to identify the progenitor cells. The second results chapter investigated the potential of p63 in identifying the progenitor population of the limbal epithelium. The use of RT-PCR enabled a more detailed examination of the RNA expression of the p63 isoforms. The experiments performed assessed p63 in situ and in vitro, including conditions designed to expand the progenitor population in culture. This work confirms the value of p63 as a marker for immature limbal epithelium and also demonstrates for the first time the p63 isoforms produced by human limbal epithelial cells in vitro. The final results chapter describes the application of techniques developed and utilised in the previous 2 chapters upon autologous cell samples and constructed grafts used to treat 5 patients clinically. The phenotypic analyses demonstrate that both the cultivated epithelium and the grafts created from the different donor biopsies exhibit similar properties in the areas examined. In addition, these results allowed comparison between clinically used autologous cultures and the methods employed to cultivate limbal epithelium examined in the previous chapter. The use of both p63 and the various cytokeratins in assessing the level of differentiation hold merit, however more specific markers for the stem cell population of the limbal epithelium remain elusive. The results present a deeper analysis of the material used to treat limbal stem cell deficiency and give insight into further evolution of these treatments.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
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Shortt, A. J. "The limbal epithelial stem cell niche and its relevance to ex-vivo culture and transplantation of corneal limbal epithelial stem cells." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/18929/.

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Анотація:

Transplantation of ex-vivo cultured limbal epithelial cells (LEC) is an established treatment for total limbal stem cell deficiency. However, this therapy has preceded the scientific understanding of limbal epithelial stem cells (LESC), the LESC niche and the biological mechanism that underpins it. This body of work tested the hypothesis that the LESC niche has a specialised structure and that an understanding of this may lead to improvements in outcomes and understanding of this therapy. The corneal limbus was investigated using 3D confocal microscopy, scanning electron microscopy, wholemount immunofluorescence and in-vivo confocal microscopy. This produced the most comprehensive study of limbal architecture performed to date. The structure of the LESC niche was identified and the effect of age and disease examined. Next, a clinical study of ex-vivo expansion and transplantation of LEC was performed in patients with LESC deficiency. A defined set of objective outcome measures enabled a baseline standard for outcomes of this therapy to be described. Future improvements to this therapy can be assessed using these techniques. Human amniotic membrane (HAM) is the leading candidate for a surrogate LESC niche. The method of HAM processing was investigated and found to be critical to achieve this. A method of significantly improving the yield of LESC in culture on HAM was identified. It remains to be seen whether this will translate into improved clinical outcomes. Finally, a disconnection exists between published data indicating a good clinical outcome and data demonstrating the survival of transplanted cells. This work assessed and demonstrated the feasibility of using quantum dot nanocrystal labelling of transplanted LESC to track cells post transplantation. It is concluded that translation of these findings to modify and improve current treatment protocols may result in improvements in outcomes for patients with LESC deficiency undergoing this therapy.

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Yang, Juan. "Universal corneal epithelial-like cells derived from human embryonic stem cells in a defined, xeno-free, and albumin-free condition for cellularization of a corneal scaffold." Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3953938.

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Книги з теми "Corneal epithelial cells"

Liminga, Maria. Arachidonic Acid Metabolism in Corneal Epithelial Cells: Indentification of Lipoxygenase Isoforms (Comprehensive Summaries of Uppsala Dissertations, 222). Uppsala Universitet, 2000.

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Частини книг з теми "Corneal epithelial cells"

Di Girolamo, Nick. "Adult Human Corneal Epithelial Stem Cells." In Adult Stem Cells, 163–97. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-9569-7_7.

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Ali, Haifa, Charles Osei-Bempong, Ani Ray-Chaudhuri, Bakiah Shaharuddin, Arianna Bianchi, Mohit Parekh, and Sajjad Ahmad. "Controversies in Corneal Epithelial Stem Cell Biology." In Adult and Embryonic Stem Cells, 103–17. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-630-2_9.

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Stapleton, Fiona, Jai-Min Kim, Jason Kasses, and Mark D. P. Willcox. "Mechanisms of Apoptosis in Human Corneal Epithelial Cells." In Advances in Experimental Medicine and Biology, 827–34. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_117.

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Kolli, Sai, Majlinda Lako, Francisco Figueiredo, and Sajjad Ahmad. "Corneal Epithelial Stem Cells and Their Therapeutic Application." In Trends in Stem Cell Biology and Technology, 319–65. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-905-5_18.

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Crouch, Rosalie, Zhu Ling, and Betty J. Hayden. "Corneal Oxygen Scavenging Systems: Lysis of Corneal Epithelial Cells by Superoxide Anions." In Oxygen Radicals in Biology and Medicine, 1043–46. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5568-7_172.

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Figueiredo, Gustavo S., Hardeep Singh Mudhar, Majlinda Lako, and Francisco C. Figueiredo. "Corneal Epithelial Stem Cells: Methods for Ex Vivo Expansion." In Essentials in Ophthalmology, 77–97. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-01304-2_6.

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Srinivas, S. P., R. Mutharasan, and S. Fleiszig. "Shear-Induced ATP Release by Cultured Rabbit Corneal Epithelial Cells." In Advances in Experimental Medicine and Biology, 677–85. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_95.

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Palmero, Mercedes, Alfonso Blanco, Juan L. Bellot, Nuria Alcoriza, Irene Perez, and Alfredo Orts. "Ultraviolet Light-Induced Damage in Rabbit Corneal Epithelial Cells in Vitro." In Advances in Ocular Toxicology, 63–72. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5937-5_7.

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Katikireddy, Kishore Reddy, and Ula V. Jurkunas. "Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells." In Embryonic Stem Cell Protocols, 437–44. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_229.

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Igarashi, Tsutomu, Koichi Miyake, Noriko Suzuki, Hiroshi Takahashi, and Takashi Shimada. "In Vivo Gene Transfer Into Corneal Epithelial Progenitor Cells By Viral Vectors." In Advances in Experimental Medicine and Biology, 1309–14. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_190.

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Тези доповідей конференцій з теми "Corneal epithelial cells"

Molladavoodi, Sara, John B. Medley, Maud Gorbet, and H. J. Kwon. "Mechanotransduction in Corneal Epithelial Cells." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65406.

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Mechanical properties of the cornea can be affected by diseases such as keratoconus. In keratoconus, a decrease in both thickness and rigidity of the cornea is observed. It is currently not clear whether and how changes in mechanical properties of the cornea are associated with corneal epithelial cell behavior. In the present study, polyacrylamide (PAA) gels with different elastic moduli have been prepared and human corneal epithelial cells (HCECs) have been cultured on them. To investigate the effect that changes in elastic modulus may have on adhesion and migration of corneal epithelial cells, actin filament organization and expression of adhesion molecules were characterized. It was found that HCECs actin filament organization improves with increasing substrate stiffness and integrin α3 expression significantly increases on more compliant substrates.

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Bandekar, N., A. Wong, D. Clausi, and M. Gorbet. "A novel approach to automated cell counting for studying human corneal epithelial cells." In 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6091482.

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Akiba, M., A. Kubota, C. Reisman, Y. Fukuma, K. Nishida, and K. P. Chan. "Evaluation of cultured corneal epithelial cells and epithelial thickness by full-field optical coherence tomography." In Biomedical Optics. Washington, D.C.: OSA, 2008. http://dx.doi.org/10.1364/biomed.2008.bmd71.

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Nam, Eunryel, and Shoji Takeuchi. "Volatile odorant detection by corneal epithelial cells using a perfusable fluidic chamber." In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2017. http://dx.doi.org/10.1109/memsys.2017.7863429.

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Fernández-Ferreiro, A., M. González-Barcia, L. Garcia-Quintanilla, A. Luaces, V. Díaz-Tome, I. Alonso-Rodriguez, X. Garcia-Otero, M. Lamas, and FJ Otero-Espinar. "PP-016 Effect of tacrolimus eye drops on human primary corneal epithelial cells." In 22nd EAHP Congress 22–24 March 2017 Cannes, France. British Medical Journal Publishing Group, 2017. http://dx.doi.org/10.1136/ejhpharm-2017-000640.463.

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Boadi, J., V. Sangwal, S. MacNeil, and S. J. Matcher. "System for tracking transplanted limbal epithelial stem cells in the treatment of corneal stem cell deficiency." In SPIE BiOS, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2015. http://dx.doi.org/10.1117/12.2077921.

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Boadi, Joseph, Stephen J. Matcher, Sheila MacNeil, and Virender S. Sangwan. "System for tracking transplanted limbal epithelial stem cells in the treatment of corneal stem cell deficiency (Conference Presentation)." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2016. http://dx.doi.org/10.1117/12.2211308.

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Cobb, Jessica A., Alison C. Dunn, Jiwoon Kwan, Malisa Sarntinoranont, Gregory Sawyer, and Roger Tran-Son-Tay. "A Novel Method for Frictional Testing on Adherent Cells." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176552.

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Understanding the physical interaction between biomaterials and tissues within the body is critical for the development of medical devices. For instance, the coefficient of friction between the two must be low enough to avoid damaging the cells that make up the tissue. Therefore, a method for obtaining friction data on cells is needed. In the present work, human corneal epithelial (HCE-T) cells are cultured within specialized cell holders and friction experiments are performed with a micro-tribometer. Cell damage is assessed via microscopy and a trypan blue viability assay. Preliminary experiments provide data for an intact monolayer of HCE-Ts being abraded by the glass pin of the micro-tribometer. The coefficient of friction for the glass pin rubbing on an intact cell layer is 0.037 ± 0.016. Additionally, these data show a direct correlation between the friction coefficients obtained and the amount of cell damage that occurs. The protocols developed thus far can be used to conduct friction testing on various adherent cell types (e.g.epithelial cells, endothelial cells, etc.). Furthermore, the micro-tribometer set-up can be modified in order to obtain friction coefficient measurements between cells and various biomaterials.

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Masters, Barry R. "Optical Biopsy of Ocular Tissue with Two-Photon Excitation Laser Scanning Microscopy." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ft7.

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Two-photon excitation laser scanning microscopy is used to produce three-dimensional maps of cellular metabolism based on the fluorescence of the naturally occurring reduced pyridine nucleotides NAD(P)H. The fluorescence from NAD(P)H was imaged with submicron lateral resolution through the 400 micron thickness of the cornea. Metabolic imaging with two-photon excitation scanning laser microscopy with near-infrared excitation has several advantages over conventional ultraviolet light. The near infrared light can penetrate deeper into the ocular tissue, there is reduced photodamage, and the chromatic aberration that occurred with ultraviolet excitation light is eliminated. In order to confirm that the fluorescence intensity is predominately from the NAD(P)H, the tissues were incubated with cyanide. A subsequent time dependent doubling of the intensity of fluorescence resulted. All cell types in the cornea of an ex vivo eye were imaged. The fluorescence from keratocytes in the corneal stroma was only observed after cyanide treatment. NAD(P)H fluorescence from lens epithelial cells was observed as well as from the lens fibers. These studies demonstrate the validity of using two-photon excitation laser scanning microscopy to perform a noninvasive optical biopsy of ocular tissue.

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Mauck, Robert L., Pen-hsiu G. Chao, Beth Gilbert, Wilmot B. Valhmu, and Clark T. Hung. "Chondrocyte Translocation and Orientation to Applied DC Electric Fields." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0405.

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Abstract Chemical and mechanical stimuli are known to cause directed movement in a number of different cell types. Less prominently studied, direct current (DC) electric fields are known to induce a similar response. In this study, we report on DC electric field-induced chondrocyte migration and re-orientation. Galvanotaxis and galvanotropism, migration and shape change in response to applied DC electric fields, respectively, have been demonstrated in many cells. For instance, field strengths of 1–10 V/cm have been reported to induce migration in keratinocytes. corneal epithelial cells, bone cells, fibroblasts and neural cells [1,7,8,11]. Recently, we have demonstrated for the first time that chondrocytes exhibit a galvanotactic response, realigning and migrating in response to applied DC electric fields (6 V/cm) [6]. In cartilage, chondrocytes may see electric fields associated with streaming potentials estimated to be up to 15 V/cm with current densities of up to 0.1A/cm2 [2]. The aim of this study was to explore basic science aspects of directed cell migration under applied DC electric fields and to investigate the potential application of this phenomena for tissue engineering, healing and repair of cartilage. The ability to direct cell growth and function will have significant implications on the bioengineering of replacement tissues.

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