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Статті в журналах з теми "Continuous cell line"

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Автор: Grafiati

Опубліковано: 10 грудня 2022

Оновлено: 28 січня 2023

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1

Hiraga, H., Takayuki Nojima, Syuiti Abe, Katsushige Yamashiro, Shinya Yamawaki, Kiyoshi Kaneda, and Kazuo Nagashima. "Establishment of a new continuous clear cell sarcoma cell line." Virchows Archiv 431, no. 1 (July 4, 1997): 45–51. http://dx.doi.org/10.1007/s004280050068.

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2

Ward, G. B., T. J. Kelly, C. W. Woods, and E. P. Marks. "Ecdysteroid production by a continuous insect cell line." Archives of Insect Biochemistry and Physiology 5, no. 2 (June 1987): 91–98. http://dx.doi.org/10.1002/arch.940050204.

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3

KOMATSUBARA, Kazunori, Yuji SIMOGONYA, and Noriyuki KATAOKA. "Effect of long-term continuous rotational stimulation on osteoblast-like cell line." Proceedings of the JSME Conference on Frontiers in Bioengineering 2020.31 (2020): 2A22. http://dx.doi.org/10.1299/jsmebiofro.2020.31.2a22.

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4

Ammar, Ayman A., and Ahmed M. Wahab. "Development of a Continuous Cell Line from Tilapia Liver." Journal of Ecology of Health & Environment 7, no. 1 (January 1, 2019): 1–5. http://dx.doi.org/10.18576/jehe/070101.

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5

Sherwood, J. B., and D. Shouval. "Continuous production of erythropoietin by an established human renal carcinoma cell line: development of the cell line." Proceedings of the National Academy of Sciences 83, no. 1 (January 1, 1986): 165–69. http://dx.doi.org/10.1073/pnas.83.1.165.

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6

MITSUHASHI, JUN, and AKIKO SHOZAWA. "Continuous Cell Lines from Larval Hemocytes of the Cabbage Armyworm, Mamestra brassiae. (continuous cell line/hemocytes/Mamestra brassicae)." Development, Growth and Differentiation 27, no. 5 (October 1985): 599–606. http://dx.doi.org/10.1111/j.1440-169x.1985.00599.x.

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7

Shimomura, S., N. Komatsu, N. Frickhofen, S. Anderson, S. Kajigaya, and NS Young. "First continuous propagation of B19 parvovirus in a cell line." Blood 79, no. 1 (January 1, 1992): 18–24. http://dx.doi.org/10.1182/blood.v79.1.18.18.

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Анотація:

Abstract The pathogenic human parvovirus B19 has extreme tropism for human erythroid progenitor cells and has resisted cultivation in conventional cell lines. We report first propagation of this virus in an erythropoietin-dependent strain of a megakaryoblastic leukemia cell line called UT-7. Virus protein was present in about 5% of cells after 1 week of culture. Appropriate ratios of major and minor capsid proteins were determined by immunoblot, and newly synthesized capsid protein was detected by immunoprecipitation of radioactively labeled cell lysates. High molecular weight monomer and dimer intermediates were detected by Southern analysis, indicating active viral replication. Approximately 1,000 genome copies were present per infected cell, and at the optimal multiplicity of infection 20- to 50- fold more virus was produced than inoculated. Virus propagation only occurred in UT-7 cells that were adapted to growth in erythropoietin; virus signal was not detected in UT-7 cells adapted for growth in granulocyte-macrophage colony-stimulating factor or interleukin-3, even with exposure to erythropoietin for several days. Infectious virus was detected in cultures as long as 3 months after inoculation. Despite persistence, there was no evidence of viral integration on Southern analysis. This cell line may prove useful for the production of infectious virus and in the analysis of B19 parvovirus persistence, cytotoxicity, and permissivity.

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8

Shimomura, S., N. Komatsu, N. Frickhofen, S. Anderson, S. Kajigaya, and NS Young. "First continuous propagation of B19 parvovirus in a cell line." Blood 79, no. 1 (January 1, 1992): 18–24. http://dx.doi.org/10.1182/blood.v79.1.18.bloodjournal79118.

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Анотація:

The pathogenic human parvovirus B19 has extreme tropism for human erythroid progenitor cells and has resisted cultivation in conventional cell lines. We report first propagation of this virus in an erythropoietin-dependent strain of a megakaryoblastic leukemia cell line called UT-7. Virus protein was present in about 5% of cells after 1 week of culture. Appropriate ratios of major and minor capsid proteins were determined by immunoblot, and newly synthesized capsid protein was detected by immunoprecipitation of radioactively labeled cell lysates. High molecular weight monomer and dimer intermediates were detected by Southern analysis, indicating active viral replication. Approximately 1,000 genome copies were present per infected cell, and at the optimal multiplicity of infection 20- to 50- fold more virus was produced than inoculated. Virus propagation only occurred in UT-7 cells that were adapted to growth in erythropoietin; virus signal was not detected in UT-7 cells adapted for growth in granulocyte-macrophage colony-stimulating factor or interleukin-3, even with exposure to erythropoietin for several days. Infectious virus was detected in cultures as long as 3 months after inoculation. Despite persistence, there was no evidence of viral integration on Southern analysis. This cell line may prove useful for the production of infectious virus and in the analysis of B19 parvovirus persistence, cytotoxicity, and permissivity.

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9

Fabbri, S., F. Marini, C. Mavilia, R. Zonefrati, G. Galli, A. Tanini, M. L. Brandi, and A. R. Gomes. "PTH-C1: A parathyroid continuous cell line derived from rat." Bone 51, no. 6 (December 2012): S28. http://dx.doi.org/10.1016/j.bone.2012.08.102.

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10

Koyama, Hiroyuki, Yasutaka Imamura, Hiroyasu Yoshikawa, Osamu Kajikawa, Shigeyoshi Itohara, Yoshio Mizuno, Takashi Yoshikawa, and Hiroshi Saito. "Establishment of a Continuous Cell Line Derived from Leukaemic Cattle." Journal of Veterinary Medicine, Series B 33, no. 1-10 (January 12, 1986): 586–96. http://dx.doi.org/10.1111/j.1439-0450.1986.tb00073.x.

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11

Stringfellow, D. A., B. W. Gray, M. Toivio-Kinnucan, P. Galik, K. P. Riddell, K. V. Brock, and R. J. Kemppainen. "A continuous cell line established from a preimplantation bovine embryo." Theriogenology 35, no. 1 (January 1991): 275. http://dx.doi.org/10.1016/0093-691x(91)90251-8.

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12

Palli, S. R., G. F. Caputo, S. S. Sohi, A. J. Brownwright, T. R. Ladd, B. J. Cook, M. Primavera, B. M. Arif, and A. Retnakaran. "CfMNPV BlocksAcMNPV-Induced Apoptosis in a Continuous Midgut Cell Line." Virology 222, no. 1 (August 1996): 201–13. http://dx.doi.org/10.1006/viro.1996.0411.

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13

Lee, Suni, Hidenori Matsuzaki, Megumi Maeda, Shoko Yamamoto, Naoko Kumagai-Takei, Tamayo Hatayama, Miho Ikeda, Kei Yoshitome, Yasumitsu Nishimura, and Takemi Otsuki. "Accelerated cell cycle progression of human regulatory T cell-like cell line caused by continuous exposure to asbestos fibers." International Journal of Oncology 50, no. 1 (November 22, 2016): 66–74. http://dx.doi.org/10.3892/ijo.2016.3776.

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14

Arrevillaga-Boni, Gerardo, Marcela Hernández-Ruiz, Elena Cristina Castillo, and Vianney Ortiz-Navarrete. "Intercellular Communication Through Contacts Between Continuous Pseudopodial Extensions in a Macrophage-like Cell Line." Cell Communication & Adhesion 21, no. 4 (June 4, 2014): 213–20. http://dx.doi.org/10.3109/15419061.2014.923993.

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15

Volkova, I., L. Reshotko, T. Bova, O. Dmytruk, and S. Derevianko. "Cultivation of potato leafroll virus (PLRV) in mammalian continuous cell lines." Agricultural Science and Practice 5, no. 3 (December 15, 2018): 19–26. http://dx.doi.org/10.15407/agrisp5.03.019.

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Анотація:

Aim. To use the ability of potato leafroll virus (PLRV) to infect and multiply in mammalian continuous cell lines to purify PLRV isolates from the vegetative plant material, and to study the pathogenicity of those isolates for plants (after culturing in mammalian continuous cell line), to investigate morphological, physical-chemical, biological and antigen properties of PLRV isolates from mammalian cells and to study an alternative diagnostic method – the neutralization test in the mammalian continuous cell lines. Methods. The methods of cultivating animal viruses in the mammalian continuous cell line, microscopical biochemical, and serological methods, the method of artifi cial nutrition of aphids are detailed under Material and Methods. Results. It was demonstrated that successful cultivation of PLRV in mammalian continuous cell line allowed obtaining pure virus isolates from potato plants and aphids and preserving them for a long time (over a period of 7 years). The cultivation of PLRV in the mammalian continuous cell line did not impact its pathogenic properties and allowed transmitting the virus to plants. Continuous cells lines of pig embryonic kidney (PEKV), of kidney Syrian hamster (BHK- 21), of testicles of piglets (PTP), of kidneys of the bull (MDBC), and of carcinoma rabbit kidney (RK-13) were found to be sensitive to PLRV, Con tinuous cell lines of human (HeLa, Hep-2 and of African green monkey kidney (Vero) were not infected by the virus. The infectious activity of PLRV in the sensitive continuous cell lines was 20–8.5 lg TCD 50 /ml depending on the cell line. The isolates of PLRV were resistant to lipid- dissolving solvents, multiplied in a pH range from 4.0 till 10.0 and were thermoresistant at 50 oС in the absence of bivalent ions of magnesium, ТIP was in the range of 60–65 oС under our experimental conditions. The optimal temperature for the reproduction of PLRV in the cell culture was c. 24 °С. The use of neutralization test in the mammalian continuous cell line allowed isolation in pure culture and identifi cation of PLRV reliably in a time span of c. 14 days. Conclusions. It was proven that PLRV can be cultivated in the mammalian continuous cell lines of PEKV, ВНК-21, PTV, MDВК and RK-13. It was established that the cultivation of PLRV in these continuous cell lines did not impact its biological, pathogenic, antigenic and physical-chemical properties. The identifi cation of pure cultures of PLRV obtained in mammalian cells can be reliably performed by the use of neutralization reaction.

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16

Zeh, Nikolas, Patrick Schlossbauer, Nadja Raab, Florian Klingler, René Handrick, and Kerstin Otte. "Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity." Metabolic Engineering Communications 13 (December 2021): e00181. http://dx.doi.org/10.1016/j.mec.2021.e00181.

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17

Gamarra, Maria Liz, Maria Carolina Maciel Albuquerque, Anderson Junger Teodoro, Renata Brum Martucci, Radovan Borojevic, Fernando Portela Câmara, Maria Teresa Villela Romanos, and Norma Santos. "Susceptibility of a continuous murine cell line (GRX) to viral infection." Revista Pan-Amazônica de Saúde 2, no. 2 (June 2011): 65–69. http://dx.doi.org/10.5123/s2176-62232011000200009.

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18

GRANDICS, P., S. SZATHMARY, Z. SZATHMARY, and T. O'NEILL. "Integration of Cell Culture with Continuous, On-Line Sterile Downstream Processing." Annals of the New York Academy of Sciences 646, no. 1 Recombinant D (December 1991): 322–33. http://dx.doi.org/10.1111/j.1749-6632.1991.tb18595.x.

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19

Jaikumar, D., K. M. Read, and G. A. Tannock. "Adaptation of Marek’s disease virus to the Vero continuous cell line." Veterinary Microbiology 79, no. 1 (March 2001): 75–82. http://dx.doi.org/10.1016/s0378-1135(00)00346-1.

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20

Fabbri, Sergio, Simone Ciuffi, Valeria Nardone, Ana Rita Gomes, Carmelo Mavilia, Roberto Zonefrati, Gianna Galli, Ettore Luzi, Annalisa Tanini, and Maria Luisa Brandi. "PTH-C1: a rat continuous cell line expressing the parathyroid phenotype." Endocrine 47, no. 1 (March 14, 2014): 90–99. http://dx.doi.org/10.1007/s12020-014-0229-7.

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21

Zhang, Xin, Ying Feng, Wei-Feng Ding, Xian Li, and Cheng-Ye Wang. "A new continuous cell line from Blaps rhynchoptera Fairmaire (Coleoptera: Tenebrionidae)." In Vitro Cellular & Developmental Biology - Animal 51, no. 2 (October 3, 2014): 151–56. http://dx.doi.org/10.1007/s11626-014-9815-5.

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22

Vigerust, David J., Sherell Vick, and Virginia L. Shepherd. "Characterization of functional mannose receptor in a continuous hybridoma cell line." BMC Immunology 13, no. 1 (2012): 51. http://dx.doi.org/10.1186/1471-2172-13-51.

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23

Smith, P. A., F. E. Díaz, M. E. Rojas, S. Díaz, M. Galleguillos, and A. Carbonero. "Effect of Piscirickettsia salmonis inoculation on the ASK continuous cell line." Journal of Fish Diseases 38, no. 3 (March 21, 2014): 321–24. http://dx.doi.org/10.1111/jfd.12248.

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24

Parwani, Anil V., W. T. Flynn, Kathy L. Gadfield, and Linda J. Saif. "Serial propagation of porcine enteric calicivirus in a continuous cell line." Archives of Virology 120, no. 1-2 (March 1991): 115–22. http://dx.doi.org/10.1007/bf01310954.

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25

Melzer, Catharina, Roland Jacobs, Thomas Dittmar, Andreas Pich, Juliane von der Ohe, Yuanyuan Yang, and Ralf Hass. "Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line." International Journal of Molecular Sciences 21, no. 13 (July 3, 2020): 4752. http://dx.doi.org/10.3390/ijms21134752.

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Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 revealed some differentiation capacity and the simultaneous presence of known MSC markers CD73, CD90, and CD105 even after continuous long-term culture for more than one year and 32 passages. The expression of CD13, CD29, CD44, and CD166 were identified as further surface proteins, all of which were also simultaneously detectable in various other types of primary MSC populations derived from the umbilical cord, bone marrow, and placenta suggesting MSC-like properties in the cell line. Proliferating steady state MSC544 exhibited immune-modulatory activity similar to a subpopulation of long-term growth-inhibited MSC544 after 189d of continuous culture in confluency. This confluent connective cell layer with fibroblast-like morphology can spontaneously contract and the generated space is subsequently occupied by new cells with regained proliferative capacity. Accordingly, the confluent and senescence-associated beta-galactosidase-positive MSC544 culture with about 95% G0/G1 growth-arrest resumed re-entry into the proliferative cell cycle within 3d after sub-confluent culture. The MSC544 cells remained viable during confluency and throughout this transition which was accompanied by marked changes in the release of proteins. Thus, expression of proliferation-associated genes was down-modulated in confluent MSC544 and re-expressed following sub-confluent conditions whilst telomerase (hTERT) transcripts remained detectable at similar levels in both, confluent growth-arrested and proliferating MSC544. Together with the capability of connective cell layer formation for potential therapeutic approaches, MSC544 provide a long term reproducible human cell source with constant properties.

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26

Vélez, Juan, Liliana M. R. Silva, Faustin Kamena, Arwid Daugschies, Sybille Mazurek, Anja Taubert, and Carlos Hermosilla. "The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support Sustained Cryptosporidium parvum Proliferation." Pathogens 11, no. 1 (December 31, 2021): 49. http://dx.doi.org/10.3390/pathogens11010049.

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Cryptosporidium parvum is an important diarrhoea-associated protozoan, which is difficult to propagate in vitro. In 2017, a report described a continuous culture of C. parvum Moredun strain, in the oesophageal squamous cell carcinoma cell line COLO-680N, as an easy-to-use system for C. parvum propagation and continuous production of oocysts. Here, we report that—using the Köllitsch strain of C. parvum—even though COLO-680N cells, indeed, allowed parasite invasion and early asexual parasite replication, C. parvum proliferation decreased after the second day post infection. Considering recurring studies, reporting on successful production of newly generated Cryptosporidium oocysts in the past, and the subsequent replication failure by other research groups, the current data stand as a reminder of the importance of reproducibility of in vitro systems in cryptosporidiosis research. This is of special importance since it will only be possible to develop promising strategies to fight cryptosporidiosis and its ominous consequences for both human and animal health by a continuous and reliable methodological progress.

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27

Mahmudi-Gharoeie, N., A. Mohammadi, B. Saffar, F. Esna-Ashari, A. Foroghi, B. Alirezaee, R. Ghorbani, and ZA Sedigh. "Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus." Iranian Journal of Virology 7, no. 4 (November 1, 2013): 7–13. http://dx.doi.org/10.21859/isv.7.4.7.

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28

Lakra, W. S., and M. Goswami. "Development and characterization of a continuous cell line PSCF from Puntius sophore." Journal of Fish Biology 78, no. 4 (March 15, 2011): 987–1001. http://dx.doi.org/10.1111/j.1095-8649.2010.02891.x.

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29

Leung, Simon, and Reina Bendayan. "Uptake properties of lamivudine (3TC) by a continuous renal epithelial cell line." Canadian Journal of Physiology and Pharmacology 79, no. 1 (January 1, 2001): 59–66. http://dx.doi.org/10.1139/y00-110.

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The purpose of this study was to characterize the renal uptake properties of the cytidine analog and antiretroviral agent 3TC. The uptake of radiolabelled 3TC was measured at 37°C in a continuous porcine renal epithelial cell line (i.e., LLC-PK1 cells) grown as a monolayer on an impermeable support. 3TC (5 µM) uptake (37°C) by the monolayer cells was saturable (Km = 1.2 ± 0.2 mM) but not significantly altered by various dideoxynucleoside analog drugs, nucleosides, and nucleoside transport inhibitors, suggesting that a nucleoside transporter is not involved in 3TC uptake. A number of endogenous organic cation probes and inhibitors significantly reduced 3TC uptake by the monolayer cells. Quinine, trimethoprim (TMP), and tetraethylammonium (TEA) inhibited 3TC uptake in a dose dependent manner with IC50 values of 0.6mM, 0.63mM, and 1.9 mM, respectively. In turn, the uptake of the typical organic cation substrate TEA was inhibited by high concentrations of 3TC. An outwardly directed proton gradient significantly increased the uptake of 3TC by the monolayer cells, suggesting the involvement of a proton exchange process. Conversely, in the presence of monensin, a Na+/H+ ionophore, the uptake of 3TC was significantly reduced. These results suggest that the uptake of 3TC by a cultured renal epithelium may be mediated by an organic cation-proton exchanger. The observed clinical interaction between 3TC and trimethoprim may be explained by competition for a common renal organic cation tubular transporter.Key words: 3TC, kidney, uptake, LLC-PK1, tubular elimination.

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30

INOUE, Hajime, and Jun MITSUHASHI. "Characterization of a New Continuous Cell Line from Silkworm (Bombyx mori) Embroys." Applied Entomology and Zoology 23, no. 1 (1988): 8–14. http://dx.doi.org/10.1303/aez.23.8.

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31

Rikihisa, Y., H. Stills, and G. Zimmerman. "Isolation and continuous culture of Neorickettsia helminthoeca in a macrophage cell line." Journal of Clinical Microbiology 29, no. 9 (1991): 1928–33. http://dx.doi.org/10.1128/jcm.29.9.1928-1933.1991.

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32

Suggs, JE, MC Madden, M. Friedman, and CJ Edgell. "Prostacyclin expression by a continuous human cell line derived from vascular endothelium." Blood 68, no. 4 (October 1, 1986): 825–29. http://dx.doi.org/10.1182/blood.v68.4.825.825.

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Abstract Prostacyclin is primarily an endothelial cell product. It contributes to the important role of endothelium in maintaining the fluidity of blood by inhibiting platelet aggregation and by promoting vasodilation. Endothelial cells in culture tend to senesce, and the level of prostacyclin expression decreases. A permanent human cell line, EA.hy 926, derived from a fusion of primary endothelial cells with cells of a less differentiated line, has been found to sustain basal and stimulated levels of prostacyclin synthesis.

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33

Suggs, JE, MC Madden, M. Friedman, and CJ Edgell. "Prostacyclin expression by a continuous human cell line derived from vascular endothelium." Blood 68, no. 4 (October 1, 1986): 825–29. http://dx.doi.org/10.1182/blood.v68.4.825.bloodjournal684825.

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Анотація:

Prostacyclin is primarily an endothelial cell product. It contributes to the important role of endothelium in maintaining the fluidity of blood by inhibiting platelet aggregation and by promoting vasodilation. Endothelial cells in culture tend to senesce, and the level of prostacyclin expression decreases. A permanent human cell line, EA.hy 926, derived from a fusion of primary endothelial cells with cells of a less differentiated line, has been found to sustain basal and stimulated levels of prostacyclin synthesis.

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34

Meyers, C., M. Frattini, J. Hudson, and L. Laimins. "Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation." Science 257, no. 5072 (August 14, 1992): 971–73. http://dx.doi.org/10.1126/science.1323879.

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35

Keysary, Avi, Trevor Waner, Carmella Strenger, and Shimon Harrus. "Cultivation ofEhrlichia Canisin a Continuous BALB/C Mouse Macrophage Cell Culture Line." Journal of Veterinary Diagnostic Investigation 13, no. 6 (November 2001): 521–23. http://dx.doi.org/10.1177/104063870101300612.

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36

Helmke, Ronald J., Victor F. German, and John A. Mangos. "A continuous alveolar macrophage cell line: Comparisons with freshly derived alveolar macrophages." In Vitro Cellular & Developmental Biology 25, no. 1 (January 1989): 44–48. http://dx.doi.org/10.1007/bf02624409.

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37

Kaltoft, K., S. Bisballe, H. Fogh Rasmussen, K. Thestrup-Pedersen, K. Thomsen, and W. Sterry. "A continuous T-cell line from a patient with S�zary syndrome." Archives of Dermatological Research 279, no. 5 (June 1987): 293–98. http://dx.doi.org/10.1007/bf00431220.

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38

Khurad, Arun M., Min-Juan Zhang, Chanchal G. Deshmukh, Ravindra S. Bahekar, Ashish D. Tiple, and Chuan-Xi Zhang. "A new continuous cell line from larval ovaries of silkworm, Bombyx mori." In Vitro Cellular & Developmental Biology - Animal 45, no. 8 (April 9, 2009): 414–19. http://dx.doi.org/10.1007/s11626-009-9197-2.

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39

Willcocks, M. M., M. J. Carter, F. R. Laidler, and C. R. Madeley. "Growth and characterisation of human faecal astrovirus in a continuous cell line." Archives of Virology 113, no. 1-2 (March 1990): 73–81. http://dx.doi.org/10.1007/bf01318354.

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40

Edgell, Cora-Jean S., Jill E. Haizlip, C. Robert Bagnell, Joan P. Packenham, Paul Harrison, Barry Wilbourn, and Victoria J. Madden. "Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926." In Vitro Cellular & Developmental Biology 26, no. 12 (December 1990): 1167–72. http://dx.doi.org/10.1007/bf02623694.

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Nachtigal, Maurice, Madan L. Nagpal, Phillip Greenspan, Sidonia A. Nachtigal, and Alain Legrand. "Characterization of a continuous smooth muscle cell line derived from rabbit aorta." In Vitro Cellular & Developmental Biology 25, no. 10 (October 1989): 892–98. http://dx.doi.org/10.1007/bf02624001.

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Bain, Peter A., Rhonda G. Hutchinson, Alexandra B. Marks, Mark St J. Crane, and Kathryn A. Schuller. "Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)." Aquaculture 376-379 (February 2013): 59–63. http://dx.doi.org/10.1016/j.aquaculture.2012.11.008.

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Ito, H., T. Kameya, T. Suwa, C. Wada, and N. Kawano. "A continuous cell line (KK-2) from a supratentorial primitive neuroectodermal tumor." Acta Neuropathologica 84, no. 1 (1992): 52–58. http://dx.doi.org/10.1007/bf00427215.

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Lynn, D. E., M. F. Feldlaufer, and W. R. Lusby. "Isolation and identification of 20-hydroxyecdysone from a lepidopteran continuous cell line." Archives of Insect Biochemistry and Physiology 5, no. 2 (June 1987): 71–79. http://dx.doi.org/10.1002/arch.940050202.

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45

Manin, V. L., I. V. Vologina, and Ye A. Trofimova. "Preparation of rabbit kidney immortalized cell culture." Veterinary Science Today, no. 4 (January 13, 2021): 297–303. http://dx.doi.org/10.29326/2304-196x-2020-4-35-298-303.

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Preparation of immortalized cell lines obtained from organs and tissues of farm animals is an essential area of biotechnology. The paper presents results of continuous (immortalized) cell line preparation from a primary trypsinized cell culture of an adult rabbit kidney. Cytomorphologic analysis and karyotyping were performed during the process of subcultivation in the cell culture at passages 1, 3, 24, 31, 38, 56, 66, 75, 86, 101. Dynamics of spontaneous continuous cell line formation during long-term serial passaging was examined using standard nutrient media and fetal serum. Contrary to the known cell lines of rabbit origin (Oryctolagus cuniculus L.), immortalization was not accompanied with enhanced cell production and cell size reduction. The prepared continuous cell line in its adhesive phase was up to 200 µm in size and its productivity was about 7,000 cells/cm2. Significant differences (compared to the known cell lines) in the karyotype were detected during passaging. The formed genotype was found to be near-tetrapioid when the CCL cultural properties were stabilized at passages 66–101. The known cell lines – rabbit kidney (RK-13) and rabbit cornea (SIRC) – can be characterized as pseudotriploid basing on their karyotype. This culture demonstrated low sensitivity to viruses – causative agents of rabbit diseases and sensitivity to heterologous porcine and bovine viruses.

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46

Skladowski, Krzysztof, Trevor J. McMillan, John Peacock, Jennifer Titley, and G. Gordon Steel. "Cell-cycle progression during continuous low dose rate irradiation of a human bladder carcinoma cell line." Radiotherapy and Oncology 28, no. 3 (September 1993): 219–27. http://dx.doi.org/10.1016/0167-8140(93)90061-c.

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Skladowski, Krzysztof, Trevor J. McMillan, John Peacock, Jennifer Titley, and G. Gordon Steel. "Cell-Cycle Progression During Continuous Low Dose Rate Irradiation of a Human Bladder Carcinoma Cell Line." Medical Dosimetry 19, no. 1 (1994): 52. http://dx.doi.org/10.1016/0958-3947(94)90037-x.

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Nanda, Indrajit, Claus Steinlein, Thomas Haaf, Eva M. Buhl, Domink G. Grimm, Scott L. Friedman, Steffen K. Meurer, Sarah K. Schröder, and Ralf Weiskirchen. "Genetic Characterization of Rat Hepatic Stellate Cell Line HSC-T6 for In Vitro Cell Line Authentication." Cells 11, no. 11 (May 29, 2022): 1783. http://dx.doi.org/10.3390/cells11111783.

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Immortalized hepatic stellate cells (HSCs) established from mouse, rat, and humans are valuable in vitro models for the biomedical investigation of liver biology. These cell lines are homogenous, thereby providing consistent and reproducible results. They grow more robustly than primary HSCs and provide an unlimited supply of proteins or nucleic acids for biochemical studies. Moreover, they can overcome ethical concerns associated with the use of animal and human tissue and allow for fostering of the 3R principle of replacement, reduction, and refinement proposed in 1959 by William M. S. Russell and Rex L. Burch. Nevertheless, working with continuous cell lines also has some disadvantages. In particular, there are ample examples in which genetic drift and cell misidentification has led to invalid data. Therefore, many journals and granting agencies now recommend proper cell line authentication. We herein describe the genetic characterization of the rat HSC line HSC-T6, which was introduced as a new in vitro model for the study of retinoid metabolism. The consensus chromosome markers, outlined primarily through multicolor spectral karyotyping (SKY), demonstrate that apart from the large derivative chromosome 1 (RNO1), at least two additional chromosomes (RNO4 and RNO7) are found to be in three copies in all metaphases. Additionally, we have defined a short tandem repeat (STR) profile for HSC-T6, including 31 species-specific markers. The typical features of these cells have been further determined by electron microscopy, Western blotting, and Rhodamine-Phalloidin staining. Finally, we have analyzed the transcriptome of HSC-T6 cells by mRNA sequencing (mRNA-Seq) using next generation sequencing (NGS).

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Qian, Zhixi, Thomas R. Hanley, Lisa M. Reece, James F. Leary, Eugene D. Boland, and Paul Todd. "Continuous Flow Labeling and In-Line Magnetic Separation of Cells." Magnetochemistry 8, no. 1 (December 30, 2021): 5. http://dx.doi.org/10.3390/magnetochemistry8010005.

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There is an identified need for point-of-care diagnostic systems for detecting and counting specific rare types of circulating cells in blood. By adequately labeling such cells with immunomagnetic beads and quantum dots, they can be efficiently collected magnetically for quantification using fluorescence methods. Automation of this process requires adequate mixing of the labeling materials with blood samples. A static mixing device can be employed to improve cell labeling efficiency and eliminate error-prone laboratory operations. Computational fluid dynamics (CFD) were utilized to simulate the flow of a labeling-materials/blood mixture through a 20-stage in-line static mixer of the interfacial-surface-generator type. Optimal fluid mixing conditions were identified and tested in a magnetic bead/tumor cell model, and it was found that labeled cells could be produced at 1.0 mL/min flow rate and fed directly into an in-line magnetic trap. The trap design consists of a dual flow channel with three bends and a permanent magnet positioned at the outer curve of each bend. The capture of labeled cells in the device was simulated using CFD, finite-element analysis and magnetophoretic mobility distributions of labeled cells. Testing with cultured CRL14777 human melanoma cells labeled with anti-CD146 1.5 μm diameter beads indicated that 90 ± 10% are captured at the first stage, and these cells can be captured when present in whole blood. Both in-line devices were demonstrated to function separately and together as predicted.

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Húngaro, Talita Guerreiro Rodrigues, Marcos F. Gregnani, Thaís Alves-Silva, Florian Herse, Natalia Alenina, Michael Bader, and Ronaldo C. Araújo. "Cortisol Dose-Dependently Impairs Migration and Tube-like Formation in a Trophoblast Cell Line and Modulates Inflammatory and Angiogenic Genes." Biomedicines 9, no. 8 (August 9, 2021): 980. http://dx.doi.org/10.3390/biomedicines9080980.

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Several stimuli can change maternal hormone levels during pregnancy. These changes may affect trophoblastic cells and modulate the development of the embryo and the placental tissue itself. Changes in cortisol levels are associated with impaired trophoblast implantation and function, in addition to other pregnancy complications. This study aims to analyze the effects of low and high doses of cortisol on an extravillous trophoblast cell line, and the effects of various exposures to this hormone. SGHPL-4 cells were treated with cortisol at five doses (0–1000 nM) and two exposures (continuous: 24 h/day; and intermittent: 2 h/day). In intermittent treatment, cortisol acted mainly as an anti-inflammatory hormone, repressing gene expression of kinin B1 receptors, interleukin-6, and interleukin-1β. Continuous treatment modulated inflammatory and angiogenic pathways, significantly repressing angiogenic factors and their receptors. Cortisol affected cell migration and tube-like structures formation. In conclusion, both continuous and intermittent exposure to cortisol repressed the expression of inflammatory genes, while only continuous exposure repressed the expression of angiogenic genes, suggesting that a sustained increase in the levels of this hormone is more harmful than a high short-term increase. Cortisol also impaired tube-like structures formation, and kinin receptors may be involved in this response.

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