Дисертації з теми "Continuous cell line"
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Tric, Mircea [Verfasser], and Stefan [Akademischer Betreuer] Wölfl. "Optical in-line biosensor for long-term continuous glucose monitoring and control in cell culture / Mircea Tric ; Betreuer: Stefan Wölfl." Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://d-nb.info/1177689065/34.
Повний текст джерелаTse, Wan-wai. "A study of genomic DNA methylation in immortalized human epithelial cell lines." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41290392.
Повний текст джерелаTse, Wan-wai, and 謝韻慧. "A study of genomic DNA methylation in immortalized human epithelial cell lines." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41290392.
Повний текст джерелаZhang, Hao. "Cytogenetic and molecular alterations in immortalization of normal esophageal epithelial cells." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B32047010.
Повний текст джерелаZhang, Hao, and 張浩. "Cytogenetic and molecular alterations in immortalization of normal esophageal epithelial cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B32047010.
Повний текст джерелаDarbro, Benjamin Will. "Mechanisms of human epithelial cell immortalization and p16NK4a induced telomere independent sencescence." Diss., University of Iowa, 2007. http://ir.uiowa.edu/etd/183.
Повний текст джерелаTruesdell, Sharon. "Analysis of cell types in continuous cell lines and analysis of the Epidermal Growth Factor Receptor pathway in Drosophila." Connect to resource, 2009. http://hdl.handle.net/1811/37280.
Повний текст джерелаFuzzi, Mirko. "Riprogettazione ed ingegnerizzazione di una linea di assemblaggio in ottica Continuous Improvement: il caso Celli UK." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020.
Знайти повний текст джерелаHyde, Jennie. "The role of prolactin in regulating CCL28 expression /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd1717.pdf.
Повний текст джерелаPatel, Shakil. "The role of membrane potential dynamics in cell behaviours : investigating the membrane potential dynamics in the Jurkat and HMEC-1 cell lines using the continuous wavelet transform." Thesis, Lancaster University, 2016. http://eprints.lancs.ac.uk/84813/.
Повний текст джерелаFiorini, Eleonora. "Riprogettazione delle linee di assemblaggio in ottica di miglioramento continuo (kaizen): il caso Celli S.p.A." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
Знайти повний текст джерелаSall, Serigne Saliou. "Bilanciamento di una linea di assemblaggio e reingegnerizzazione del sistema di asservimento materiali in ottica Continuous Improvement: il caso Celli UK." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2021.
Знайти повний текст джерелаGarcía, García Alejandro. "Multiscale analysis of multi-layered tissues constructs : interfaces in the musculo-skeletal system based on tissue engineered osteotendinous junctions." Thesis, Compiègne, 2019. http://www.theses.fr/2019COMP2488.
Повний текст джерелаThe objective of this thesis was the development of a biohybrid substitute for the reconstruction of the bone-tendon continuum based on tissue engineering strategies. After an exhaustive bibliographic analysis of the native structures and their environment, we first proposed the realization of each system separately using electrospun polycaprolactone scaffolds. At first, we combined electrospinning with electrospraying techniques to produce a PCL-hydroapatite scaffold with honeycomb cavities. Our hypothesis was to provide the substitute with a biomimetic structure favoring cell adhesion, spreading and differentiation. The in vitro mechanical and biological analysis performed with a progenitor cell line and withorganotypic assays confirmed our original approach. Then, the material seeded with bone marrow stem cells was successfully implanted by our collaborators in Amiens with the objective of treating a maxillofacial defect in a rodent model. In parallel, for the tendon reconstruction, we investigated several electrospinning processes, varying fibers’ size and organization (random/aligned). In a bioinspired perspective, we combined the choice of the scaffold with dynamic stretching to reproduce physical training. Under those mechanical stimulations, established first with the same progenitor cell line, we demonstrated in a second study that MSCs aligned with the stretching axis and produced extracellular matrix, which in turn allowed to keep the mechanical properties of the biohybrid scaffold all over the 2 weeks of culture. We demonstrated that cell differentiation towards tendon and bone lineage was successfully achieved in the absence of any differentiation factor, being specifically related to materials properties and mechanotransduction. Therefore, the next step consisting in the assembly of both scaffolds with a transition area should lead to this bone-tendon continuum’s reconstruction
Chang, Jia-Rong, and 張家榮. "Establishment of a continuous cell line derived from barramundi (Lates calcarifer ) and its applications." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/52636999813684799231.
Повний текст джерела國立宜蘭大學
生物技術研究所碩士班
95
Lates calcarifer (bloch) belongs to the Lates genus, Centropomidae family, Perciformes order and Osteichthyes class. This tropical and sub-tropical fish is located in the coastal brackish water throughout Okinawa to the Indian Ocean. Lates calcarifer is massively cultured in Southeastern Asia and Southern Taiwan. Nervous Necrosis Virus (NNV), a member of the Betanodaviridae, is an icosahedral, non-enveloped RNA virus 25-30 nm in diameter. NNV mainly infects juveniles, destroying the nerves of the brain, leading to abnormal swimming and high mortality of infected fish, causing devastating economic impact to the aquaculture industry. Since the larvae of Lates calcarifier are highly susceptible to NNV, this research study developed primary and continuous cell lines from the liver, kidney, and the gas bladder of bloch. The liver and kidney cells were propagated over 90 passages while the gas bladder cell line was propagated over 150 passages, maintained in 5% FBS, L-15 medium, and grew quickly at both 28°C and 32°C. Epinephelus lanceolatus NNV was first used to test these different cell lines for susceptibility and results showed that Lates calcarifer gas bladder (LCGB) was highly susceptible. Then, wild type Plectropomus leopardus Nervous Necrosis Virus (PLNNV) was replicated in LCGB and the viral titer was 107 TCID50/mL. Large quantities of PLNNV was grown in LCGB, collected and purified, serving as the antigen for anti-NNV monoclonal antibodies production in BALB/c mice. After limiting dilution and screening, 3 different hybridomas secreting monoclonal antibodies against NNV were successfully produced. The 3 monoclonal antibodies were confirmed by Western blot and IPMA against purified NNV. Subsequently, cloning of PLNNV capsid gene and expression of recombinant proteins in E. coli was done in pQE30 vector and M15 host. A Western blot of the monoclonal antibodies against the recombinant protein proved all 3 monoclonal antibodies produced in this study were against the NNV capsid protein. These monoclonal antibodies are important tools to have for further researches on Nervous Necrosis Virus.
Devaraj, Vikram. "Modeling, design, development, and control of a pilot-scale continuous coating line for proton exchange membrane fuel cell electrode assembly." 2012. http://hdl.handle.net/2152/19835.
Повний текст джерелаtext
Chen, Po-Hua, and 陳柏華. "Continuous Monitoring of Cu, Mn, Ni and Se in Saline and Cell Suspension Using On-line Microdialysis Coupled with Simultaneous Multielement Electrothermal Atomic Absorption Spectrometry." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/01600846339030821609.
Повний текст джерела高雄醫學大學
醫學研究所碩士班
93
Microdialysis sampling coupled on-line with simultaneous electrothermal atomic absorption spectrometry (SIMAAS) for continuous monitoring of copper (Cu), manganese (Mn), nickel (Ni) and selenium (Se) in cell cultured suspension has been developed. The trace elements investigated in this study are considered to be markedly associated with oxidative stress in biomedicine. Two major works were carried out in this study. Firstly, the analytical conditions including temperature program and matrix modifiers have been developed for the determination of Cu, Mn, Ni and Se in saline solution. The results showed that 3 mg of Pd and 10 mg of Mg(NO3)2 were successfully used as the matrix modifiers to prevent target elements from evaporation and to eliminate the interference resulting from NaCl in 1200℃ of the pyrolysis step. The analytical performances such as linearity, accuracy, and precision were validated to confirm the reliability of the proposed method. Secondly, the utrapure saline solution (0.9% NaCl) was used as the perfusion solution at the flow rate of 1 mL/min through the microdialysis probe to develop the on-line microdialysis-SIMAAS system. The optimized conditions of on-line microdialysis- SIMAAS system were performed for continuous measurements of four trace elements in microdialysate. The probe recovery of on-line microdialysis-SIMAAS system measured by a saline solution contained Cu, Mn, Ni and Se was 57.9 ± 1.4%, 65.0 ± 1.0%, 65.5 ± 4.1%, and 67.9 ± 4.6%, respectively. The analytical performances for microdialysis system such as long-term stability, response, and spiked recovery were validated to confirm the reliability of on-line system. The use of an on-line microdialysis-SIMAAS system permitted the in situ, dynamic and continuous monitoring of extracellular diffusible Cu, Mn, Ni and Se in cell suspension after treated with trace elements.
Maduray, Kaminee. "In vitro toxicity testing of phthalocyanines on different cell lines using a continuous laser source." Thesis, 2010. http://hdl.handle.net/10321/550.
Повний текст джерелаPhotodynamic therapy is a promising treatment for cancer. It involves the combination of a photosensitizer and light of an appropriate wavelength (laser source) to cause the destruction of cancer cells. Phthalocynanines are second–generation photosensitizers with enhanced photophysical and photochemical properties. In this in vitro study the effect of aluminium (AlTSPc) or zinc (ZnTSPc) tetrasulfophthalocyanines in its inactive and active state (laser induced) on melanoma (skin cancer cells), fibroblast (healthy normal skin cells) and keratinocyte (healthy normal skin cells) cells was evaluated. For each of the cell lines approximately 3 x 104 cells/ml were seeded onto 24-well cell culture plates and allowed to attach overnight, after which cells were treated with different concentrations of AlTSPc or ZnTSPc. The photosensitizers were synthesized at Rhodes University. After 2 hrs, cells were irradiated with a diode laser at a wavelength of 672 nm and a beam diameter of 1 cm. The laser power varied between 20-30 mW and the irradiation time was calculated to deliver a light dose of 4.5 J/cm2. Post-irradiated cells were incubated for 24 hrs before cell viability was measured using the CellTiter-BlueTM Viability Assay. Also, the efficacy of the light dose and laser source used for the killing of approximately 50% of the melanoma cancer cells were investigated. AlTSPc and ZnTSPc decreased cell viability of melanoma cancer cells to approximately 50% with photosensitizer concentrations of 40 μg/ml and 50 μg/ml respectively. These photosensitizer concentrations caused a slight decrease in the percentage cell viability of fibroblast and keratinocyte cells. Results for the dark toxicity assay showed that iii both photosensitizers in the presence of high concentrations (60 μg/ml – 100 μg/ml) showed cytotoxicity effects on melanoma cancer cells in their inactive state. This was not observed in fibroblast and keratinocyte cells treated under the same experimental conditions. The optimal AlTSPc and ZnTSPc concentrations in combination with the light dose of 4.5 J/cm2 was the most efficient in killing the melanoma cancer cells with reduced killing effects on healthy normal fibroblast and keratinocyte cells when compared to other light doses (2.5 J/cm2, 7.5 J/cm2 and 10 J/cm2). The irradiation of cells photosensitized with the optimal photosensitizer concentrations with a femtosecond laser using similar laser parameters to continuous wave laser experiments resulted in a reduction in the cell viability of healthy normal fibroblast and keratinocyte cells compared to melanoma cancer cells. The presence of DNA degradation on agarose gel, morphological changes like blebbing and ultrastructural changes like nucleus condensation indicated that photodynamic therapy treated melanoma cancer cells with the optimal concentrations of AlTSPc and ZnTSPc induced cell death via apoptosis. This concludes that low concentrations of AlTSPc and ZnTSPc activated with an appropriate laser source can be used to induce cell death in melanoma cancer cells. Both AlTSPc and ZnTSPc exhibit the potential to be used as a photosensitizer in photodynamic therapy for the treatment of melanoma cancer with the occurrence of minimal damage to surrounding healthy tissue.
Kam, Sarah Anne. "Development of models for the study of anesthetic preconditioning using rat pheochromocytoma and mouse neuroblastoma." 2009. http://hdl.handle.net/10090/8913.
Повний текст джерела陳慧如. "Establishment of a continuous cell lines from swim bladder of grouper (Epinephelus coioides) and its application on study of TGIV (grouper iridovirus of Taiwan)." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/82155519543121262623.
Повний текст джерелаDe, Lange Beverley. "A visual interpretation of consciousness as a continuous process of self-organisation and embodiment." Thesis, 2019. http://hdl.handle.net/10500/26544.
Повний текст джерелаArt History, Visual Arts and Musicology
D. Litt. et Phil. (Art History)