Статті в журналах: "Connexion internet"

1

Baltezarevic, Radoslav, Borivoje Baltezarevic, Piotr Kwiatek, and Vesna Baltezarevic. "The Impact of Virtual Communities on Cultural Identity." Symposion 6, no. 1 (2019): 7–22. http://dx.doi.org/10.5840/symposion2019611.

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The emergence of the Internet and various forms of virtual communities has led to the impact of a new social space on individuals who frequently replace the real world with alternative forms of socializing. In virtual communities, new ‘friendships’ are easily accepted; however, how this acceptance influences cultural identity has not been investigated. Based on the data collected from 443 respondents in the Republic of Serbia, authors analyze this connexion, as well as how the absorption of others’ cultural values is reflected on the local cultural values. The results show that the adoption of others’ cultural values diminished the bond with the local community. The present paper adds to the theory of virtual communities by examining the relationship between the acceptance of an unknown person in a virtual community and its effects on cultural identity. This study contributes to the clarification of the impact that virtual networking has on cultural identity.

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2

Nielsen, Peter A., Amos Baruch, Valery I. Shestopalov, Ben N. G. Giepmans, Irene Dunia, E. Lucio Benedetti та Nalin M. Kumar. "Lens Connexins α3Cx46 and α8Cx50 Interact with Zonula Occludens Protein-1 (ZO-1)". Molecular Biology of the Cell 14, № 6 (червень 2003): 2470–81. http://dx.doi.org/10.1091/mbc.e02-10-0637.

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Connexin α1Cx43 has previously been shown to bind to the PDZ domain–containing protein ZO-1. The similarity of the carboxyl termini of this connexin and the lens fiber connexins α3Cx46 and α8Cx50 suggested that these connexins may also interact with ZO-1. ZO-1 was shown to be highly expressed in mouse lenses. Colocalization of ZO-1 with α3Cx46 and α8Cx50 connexins in fiber cells was demonstrated by immunofluorescence and by fracture-labeling electron microscopy but showed regional variations throughout the lens. ZO-1 was found to coimmunoprecipitate with α3Cx46 and α8Cx50, and pull-down experiments showed that the second PDZ domain of ZO-1 was involved in this interaction. Transiently expressed α3Cx46 and α8Cx50 connexins lacking the COOH-terminal residues did not bind to the second PDZ domain but still formed structures resembling gap junctions by immunofluorescence. These results indicate that ZO-1 interacts with lens fiber connexins α3Cx46 and α8Cx50 in a manner similar to that previously described for α1Cx43. The spatial variation in the interaction of ZO-1 with lens gap junctions is intriguing and is suggestive of multiple dynamic roles for this association.

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3

Kopanic, Jennifer L., Barbara Schlingmann, Michael Koval, Alan F. Lau, Paul L. Sorgen, and Vivian F. Su. "Degradation of gap junction connexins is regulated by the interaction with Cx43-interacting protein of 75 kDa (CIP75)." Biochemical Journal 466, no. 3 (March 6, 2015): 571–85. http://dx.doi.org/10.1042/bj20141042.

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Connexins are a family of transmembrane proteins that form gap junction channels. These proteins undergo both proteasomal and lysosomal degradation, mechanisms that serve to regulate connexin levels. Our previous work described CIP75 [connexin43 (Cx43)-interacting protein of 75 kDa], a protein involved in proteasomal degradation, as a novel Cx43-interacting protein. We have discovered two additional connexins, connexin40 (Cx40) and connexin45 (Cx45), that interact with CIP75. Nuclear magnetic resonance (NMR) analyses identified the direct interaction of the CIP75 UBA domain with the carboxyl-terminal (CT) domains of Cx40 and Cx45. Reduction in CIP75 by shRNA in HeLa cells expressing Cx40 or Cx45 resulted in increased levels of the connexins. Furthermore, treatment with trafficking inhibitors confirmed that both connexins undergo endoplasmic reticulum-associated degradation (ERAD), and that CIP75 preferentially interacts with the connexin proteins bound for proteasomal degradation from the ER. In addition, we have also discovered that CIP75 interacts with ER-localized Cx32 in a process that is likely mediated by Cx32 ubiquitination. Thus, we have identified novel interacting connexin proteins of CIP75, indicating a role for CIP75 in regulating the levels of connexins in general, through proteasomal degradation.

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4

Evans, W. Howard, Elke De Vuyst, and Luc Leybaert. "The gap junction cellular internet: connexin hemichannels enter the signalling limelight." Biochemical Journal 397, no. 1 (June 14, 2006): 1–14. http://dx.doi.org/10.1042/bj20060175.

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Cxs (connexins), the protein subunits forming gap junction intercellular communication channels, are transported to the plasma membrane after oligomerizing into hexameric assemblies called connexin hemichannels (CxHcs) or connexons, which dock head-to-head with partner hexameric channels positioned on neighbouring cells. The double membrane channel or gap junction generated directly couples the cytoplasms of interacting cells and underpins the integration and co-ordination of cellular metabolism, signalling and functions, such as secretion or contraction in cell assemblies. In contrast, CxHcs prior to forming gap junctions provide a pathway for the release from cells of ATP, glutamate, NAD+ and prostaglandin E2, which act as paracrine messengers. ATP activates purinergic receptors on neighbouring cells and forms the basis of intercellular Ca2+ signal propagation, complementing that occuring more directly via gap junctions. CxHcs open in response to various types of external changes, including mechanical, shear, ionic and ischaemic stress. In addition, CxHcs are influenced by intracellular signals, such as membrane potential, phosphorylation and redox status, which translate external stresses to CxHc responses. Also, recent studies demonstrate that cytoplasmic Ca2+ changes in the physiological range act to trigger CxHc opening, indicating their involvement under normal non-pathological conditions. CxHcs not only respond to cytoplasmic Ca2+, but also determine cytoplasmic Ca2+, as they are large conductance channels, suggesting a prominent role in cellular Ca2+ homoeostasis and signalling. The functions of gap-junction channels and CxHcs have been difficult to separate, but synthetic peptides that mimic short sequences in the Cx subunit are emerging as promising tools to determine the role of CxHcs in physiology and pathology.

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5

Foote, Cynthia I., Lan Zhou, Xing Zhu, and Bruce J. Nicholson. "The Pattern of Disulfide Linkages in the Extracellular Loop Regions of Connexin 32 Suggests a Model for the Docking Interface of Gap Junctions." Journal of Cell Biology 140, no. 5 (March 9, 1998): 1187–97. http://dx.doi.org/10.1083/jcb.140.5.1187.

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Connexins, like true cell adhesion molecules, have extracellular domains that provide strong and specific homophilic, and in some cases, heterophilic interactions between cells. Though the structure of the binding domains of adhesion proteins have been determined, the extracellular domains of connexins, consisting of two loops of ∼34–37 amino acids each, are not easily studied in isolation from the rest of the molecule. As an alternative, we used a novel application of site-directed mutagenesis in which four of the six conserved cysteines in the extracellular loops of connexin 32 were moved individually and in all possible pairwise and some quadruple combinations. This mapping allowed us to deduce that all disulfides form between the two loops of a single connexin, with the first cysteine in one loop connected to the third of the other. Furthermore, the periodicity of movements that produced functional channels indicated that these loops are likely to form antiparallel β sheets. A possible model that could explain how these domains from apposed connexins interact to form a complete channel is discussed.

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6

Yeh, Hung-I., Emmanuel Dupont, Steven Coppen, Stephen Rothery, and Nicholas J. Severs. "Gap Junction Localization and Connexin Expression in Cytochemically Identified Endothelial Cells of Arterial Tissue." Journal of Histochemistry & Cytochemistry 45, no. 4 (April 1997): 539–50. http://dx.doi.org/10.1177/002215549704500406.

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Vascular endothelial cells interact with one another via gap junctions, but information on the precise connexin make-up of endothelial gap junctions in intact arterial tissue is limited. One factor contributing to this lack of information is that standard immunocytochemical methodologies applied to arterial sections do not readily permit unequivocal localization of connexin immunolabeling to endothelium. Here we introduce a method for multiple labeling with specific endothelial cell markers and one or more connexin-specific antibodies which overcomes this limitation. Applying this method to localize connexins 43, 40, and 37 by confocal microscopy, we show that the three connexin types have quite distinctive labeling patterns in different vessels. Whereas endothelial cells of rat aorta and coronary artery characteristically show extensive, prominent connexin40, and heterogeneous scattered connexin37, the former, unlike the latter, also has abundant connexin43. The relative lack of connexin43 in coronary artery endothelium was confirmed in both rat and human using three alternative antibodies. In the aorta, connexins43 and 40 commonly co-localize to the same junctional plaque. Even within a given type of endothelium, zonal variation in connexin expression was apparent. In rat endocardium, a zone just below the mitral valve region is marked by expression of greater quantities of connexin43 than surrounding areas. These results are consistent with the idea that differential expression of connexins may contribute to modulation of endothelial gap junction function in different segments and subzones of the arterial system.

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7

Bruzzone, R., J. A. Haefliger, R. L. Gimlich, and D. L. Paul. "Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins." Molecular Biology of the Cell 4, no. 1 (January 1993): 7–20. http://dx.doi.org/10.1091/mbc.4.1.7.

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The cellular distribution of connexin40 (Cx40), a newly cloned gap junction structural protein, was examined by immunofluorescence microscopy using two different specific anti-peptide antibodies. Cx40 was detected in the endothelium of muscular as well as elastic arteries in a punctate pattern consistent with the known distribution of gap junctions. However, it was not detected in other cells of the vascular wall. By contrast, Cx43, another connexin present in the cardiovascular system, was not detected in endothelial cells of muscular arteries but was abundant in the myocardium and aortic smooth muscle. We have tested the ability of these connexins to interact functionally. Cx40 was functionally expressed in pairs of Xenopus oocytes and induced the formation of intercellular channels with unique voltage dependence. Unexpectedly, communication did not occur when oocytes expressing Cx40 were paired with those expressing Cx43, although each could interact with a different connexin, Cx37, to form gap junction channels in paired oocytes. These findings indicate that establishment of intercellular communication can be spatially regulated by the selective expression of different connexins and suggest a mechanism that may operate to control the extent of communication between cells.

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8

El Bettioui, Rachid, Abdelkhalil Haidane, Lhassane Jaouhari, and Samir Mirdasse. "Digitalisation pédagogique et défis de l’enseignement à distance." revistamultidisciplinar.com 4, no. 1 (2022): 27–48. http://dx.doi.org/10.23882/rmd.22083.

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La digitalisation représente, de notre temps, une nécessité de développement et de compétitivité de la majorité des secteurs. Le secteur de l’enseignement supérieur n’échappe pas de cette transformation digitale. Ainsi, au cours des deux dernières décennies, la pédagogie digitale est devenue l'une des formes d'apprentissage les plus adéquates pour la génération actuelle des étudiants de l'enseignement supérieur qui préfèrent un modèle de formation doté des smart universités. Dans ce cadre, le présent article consiste à étudier les défis de l’enseignement à distance appliqué au Maroc pendant la pandémie de COVID-19 pour maintenir la formation des étudiants. La méthode de recherche utilisée est la méthode quantitative. Ainsi, un questionnaire en ligne a été rempli par 487 étudiants de l’Ecole supérieure de technologie d’Agadir de l’université Ibn Zohr au Maroc. Les données collectées ont été analysées premièrement par la méthode descriptive (statistique descriptive uni-variée), puis par la méthode analytique en appliquant le Test de Khi-deux, à l’aide du logiciel IBM SPSS Statistics. Cette dernière a été menée pour analyser les relations entre les variables du modèle conceptuel. Les résultats de cette recherche ont révélé que l’accélération de la transformation digitale de l’enseignement supérieur fait face à plusieurs défis. Au niveau des apprenants, de nombreux étudiants éprouvent de sérieuses difficultés à utiliser les plateformes digitales de l’enseignement à savoir le problème de lenteur et de coût de connexion internet, la qualité de la vidéo et du son des vidéoconférences, la difficulté de compréhension des cours… Par conséquent, ils ne participent pas toujours à leurs classes virtuelles.

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9

Giaume, Christian, Christian C. Naus, Juan C. Sáez, and Luc Leybaert. "Glial Connexins and Pannexins in the Healthy and Diseased Brain." Physiological Reviews 101, no. 1 (January 1, 2021): 93–145. http://dx.doi.org/10.1152/physrev.00043.2018.

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Over the past several decades a large amount of data have established that glial cells, the main cell population in the brain, dynamically interact with neurons and thus impact their activity and survival. One typical feature of glia is their marked expression of several connexins, the membrane proteins forming intercellular gap junction channels and hemichannels. Pannexins, which have a tetraspan membrane topology as connexins, are also detected in glial cells. Here, we review the evidence that connexin and pannexin channels are actively involved in dynamic and metabolic neuroglial interactions in physiological as well as in pathological situations. These features of neuroglial interactions open the way to identify novel non-neuronal aspects that allow for a better understanding of behavior and information processing performed by neurons. This will also complement the “neurocentric” view by facilitating the development of glia-targeted therapeutic strategies in brain disease.

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Semeki Ngabinzeke, Jean, Mikhail Pitchugin, Julie Linchant, Cédric Vermeulen, Jean-Marie Kahindo Muhongya, and Philippe Lejeune. "Une méthode simple et rapide pour l’évaluation de statistiques d’occupation du sol à l’aide d’images à très haute résolution acquises par mini-drone." BOIS & FORETS DES TROPIQUES 335 (March 15, 2018): 15. http://dx.doi.org/10.19182/bft2018.335.a31497.

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Le suivi de l’utilisation des terres par télédétection a récemment connu un essor important. Cela s’explique par une accessibilité accrue et souvent gratuite des images à (très) haute résolution ainsi que par le développement d’applications web destinées au suivi de l’utilisation des terres. L’accès à ces applications reste cependant soumis à l’existence d’une connexion Internet fiable faisant encore défaut dans certaines régions du globe. Dans ce contexte, la présente étude décrit une méthode permettant de produire des statistiques sur l’évolution de l’occupation du sol en réalisant une photo-interprétation par point sur des images en couleurs vraies à très haute résolution produites par mini-drone. La méthode utilise une application (PINT pour Photo-INTerprétation) intégrée dans le logiciel open source QGIS. Les surfaces de différentes occupations du sol sont dérivées des estimations des proportions de points affectées à chaque classe à partir d’une grille systématique. Pour illustrer l’intérêt de l’outil, l’étude considère les statistiques d’occupation du sol au sein de deux terroirs villageois du Complexe d’aires protégées de la Garamba, en République démocratique du Congo. Les résultats obtenus sont comparés avec ceux d’une cartographie de référence basée sur une photo-interprétation exhaustive après segmentation des images. Les écarts entre surfaces estimées par échantillonnage et surfaces de référence varient entre 0,2 % et 6,1 % pour les principales occupations du sol (forêts et savanes, défriches, jachères, implantations humaines et cultures). Des différences plus importantes (17,4 % et 13,4 %) sont enregistrées pour la classe « arbres isolés ». Le temps global de mise en œuvre de la méthode est de l’ordre de 60 ha par heure d’opérateur. L’utilisation du plugin PINT avec des images « drone » constitue une solution pertinente pour estimer des statistiques d’occupation du sol dans des régions web-isolées et pour des sites d’étendues de quelques (dizaines de) km².

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11

White, T. W., R. Bruzzone, S. Wolfram, D. L. Paul, and D. A. Goodenough. "Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins." Journal of Cell Biology 125, no. 4 (May 15, 1994): 879–92. http://dx.doi.org/10.1083/jcb.125.4.879.

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Gap junctions are collections of intercellular channels composed of structural proteins called connexins (Cx). We have examined the functional interactions of the three rodent connexins present in the lens, Cx43, Cx46, and Cx50, by expressing them in paired Xenopus oocytes. Homotypic channels containing Cx43, Cx46, or Cx50 all developed high conductance. heterotypic channels composed of Cx46 paired with either Cx43 or Cx50 were also well coupled, whereas Cx50 did not form functional channels with Cx43. We also examined the functional response of homotypic and heterotypic channels to transjunctional voltage and cytoplasmic acidification. We show that all lens connexins exhibited sensitivity to cytoplasmic acidification as well as to voltage, and that voltage-dependent closure of heterotypic channels for a given connexin was dramatically influenced by its partner connexins in the adjacent cell. Based on the observation that Cx43 can discriminate between Cx46 and Cx50, we investigated the molecular determinants that specify compatibility by constructing chimeric connexins from portions of Cx46 and Cx50 and testing them for their ability to form channels with Cx43. When the second extracellular (E2) domain in Cx46 was replaced with the E2 of Cx50, the resulting chimera could no longer form heterotypic channels with Cx43. A reciprocal chimera, where the E2 of Cx46 was inserted into Cx50, acquired the ability to functionally interact with Cx43. Together, these results demonstrate that formation of intercellular channels is a selective process dependent on the identity of the connexins expressed in adjacent cells, and that the second extracellular domain is a determinant of heterotypic compatibility between connexins.

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12

De Vuyst, Elke, Elke Decrock, Marijke De Bock, Hiroshi Yamasaki, Christian C. Naus, W. Howard Evans, and Luc Leybaert. "Connexin Hemichannels and Gap Junction Channels Are Differentially Influenced by Lipopolysaccharide and Basic Fibroblast Growth Factor." Molecular Biology of the Cell 18, no. 1 (January 2007): 34–46. http://dx.doi.org/10.1091/mbc.e06-03-0182.

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Gap junction (GJ) channels are formed by two hemichannels (connexons), each contributed by the cells taking part in this direct cell–cell communication conduit. Hemichannels that do not interact with their counterparts on neighboring cells feature as a release pathway for small paracrine messengers such as nucleotides, glutamate, and prostaglandins. Connexins are phosphorylated by various kinases, and we compared the effect of various kinase-activating stimuli on GJ channels and hemichannels. Using peptides identical to a short connexin (Cx) amino acid sequence to specifically block hemichannels, we found that protein kinase C, Src, and lysophosphatidic acid (LPA) inhibited GJs and hemichannel-mediated ATP release in Cx43-expressing C6 glioma cells (C6-Cx43). Lipopolysaccharide (LPS) and basic fibroblast growth factor (bFGF) inhibited GJs, but they stimulated ATP release via hemichannels in C6-Cx43. LPS and bFGF inhibited hemichannel-mediated ATP release in HeLa-Cx43 cells, but they stimulated it in HeLa-Cx43 with a truncated carboxy-terminal (CT) domain or in HeLa-Cx26, which has a very short CT. Hemichannel potentiation by LPS was inhibited by blockers of the arachidonic acid metabolism, and arachidonic acid had a potentiating effect like LPS and bFGF. We conclude that GJ channels and hemichannels display similar or oppositely directed responses to modulatory influences, depending on the balance between kinase activity and the activity of the arachidonic acid pathway. Distinctive hemichannel responses to pathological stimulation with LPS or bFGF may serve to optimize the cell response, directed at strictly controlling cellular ATP release, switching from direct GJ communication to indirect paracrine signaling, or maximizing cell-protective strategies.

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13

Peracchia, Camillo. "Connexin/Innexin Channels in Cytoplasmic Organelles. Are There Intracellular Gap Junctions? A Hypothesis!" International Journal of Molecular Sciences 21, no. 6 (March 21, 2020): 2163. http://dx.doi.org/10.3390/ijms21062163.

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This paper proposes the hypothesis that cytoplasmic organelles directly interact with each other and with gap junctions forming intracellular junctions. This hypothesis originated over four decades ago based on the observation that vesicles lining gap junctions of crayfish giant axons contain electron-opaque particles, similar in size to junctional innexons that often appear to directly interact with junctional innexons; similar particles were seen also in the outer membrane of crayfish mitochondria. Indeed, vertebrate connexins assembled into hexameric connexons are present not only in the membranes of the Golgi apparatus but also in those of the mitochondria and endoplasmic reticulum. It seems possible, therefore, that cytoplasmic organelles may be able to exchange small molecules with each other as well as with organelles of coupled cells via gap junctions.

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14

Bai, Zi-qian, Jeanne Tan, Clare Frances Johnston, and Xiao-Ming Tao. "Connexion." International Journal of Clothing Science and Technology 27, no. 6 (November 2, 2015): 870–94. http://dx.doi.org/10.1108/ijcst-05-2014-0058.

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Purpose – The purpose of this paper is to investigate how electronic components can be utilized and integrated into polymeric optical fibre (POF) textiles to refine the design aesthetic, tactile quality and initiate the interaction of textiles with the users; and to study the design process of interactive products by using a novel design process model. Design/methodology/approach – Fashion and textile design methods, textile technology are used in combination with modern technologies such as laser engraving, sensing, short-distance communication technology, throughout the entire process of development of interactive photonics creations. Findings – The results of evaluation indicate that the engineered prototypes can enhance the interactive function of interior furnishing. The usability of interactive POF cushions is optimized by innovative design methods considering both design and technology. Originality/value – This research explores to combine knowledge from different disciplines, including textile, electronics, sensor and laser to create interactive soft furnishings. The inter-disciplinary research provides a new perspective on how POF fabric can be utilized as a new media to change the way people interact with their living surroundings. The interior soft furnishings are no longer unresponsive to people, but can react to them, adapt to their behaviors, change color according to their preferences and therefore merge into our daily life. The developed prototypes reshape interior soft furnishing, and therefore have both theoretical and practical significance.

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15

Bruzzone, Roberto, Thomas W. White, and Daniel A. Goodenough. "The cellular internet: On-line with connexins." BioEssays 18, no. 9 (September 1996): 709–18. http://dx.doi.org/10.1002/bies.950180906.

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Zou, Juan, Mani Salarian, Yanyi Chen, You Zhuo, Nicole E. Brown, John R. Hepler, and Jenny J. Yang. "Direct visualization of interaction between calmodulin and connexin45." Biochemical Journal 474, no. 24 (November 27, 2017): 4035–51. http://dx.doi.org/10.1042/bcj20170426.

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Calmodulin (CaM) is an intracellular Ca2+ transducer involved in numerous activities in a broad Ca2+ signaling network. Previous studies have suggested that the Ca2+/CaM complex may participate in gap junction regulation via interaction with putative CaM-binding motifs in connexins; however, evidence of direct interactions between CaM and connexins has remained elusive to date due to challenges related to the study of membrane proteins. Here, we report the first direct interaction of CaM with Cx45 (connexin45) of γ-family in living cells under physiological conditions by monitoring bioluminescence resonance energy transfer. The interaction between CaM and Cx45 in cells is strongly dependent on intracellular Ca2+ concentration and can be blocked by the CaM inhibitor, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W7). We further reveal a CaM-binding site at the cytosolic loop (residues 164–186) of Cx45 using a peptide model. The strong binding (Kd ∼ 5 nM) observed between CaM and Cx45 peptide, monitored by fluorescence-labeled CaM, is found to be Ca2+-dependent. Furthermore, high-resolution nuclear magnetic resonance spectroscopy reveals that CaM and Cx45 peptide binding leads to global chemical shift changes of 15N-labeled CaM, but does not alter the size of the structure. Observations involving both N- and C-domains of CaM to interact with the Cx45 peptide differ from the embraced interaction with Cx50 from another connexin family. Such interaction further increases Ca2+ sensitivity of CaM, especially at the N-terminal domain. Results of the present study suggest that both helicity and the interaction mode of the cytosolic loop are likely to contribute to CaM's modulation of connexins.

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17

Roger, Antoine. "Partis politiques et divisions sociales : les enseignements de l’Europe centrale et orientale." II. Éclairages nationaux er comportements politiques, no. 49 (June 30, 2004): 131–44. http://dx.doi.org/10.7202/007916ar.

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Résumé Les mutations intervenues depuis 1990 dans les pays d’Europe centrale et orientale appellent une redéfinition des rapports établis entre partis politiques et divisions sociales. Aucune concordance n’est pourtant perceptible au premier abord. Selon l’explication la plus répandue, les pesanteurs héritées du régime communiste font obstacle à une connexion des dynamiques politique et sociale. Cette clé de lecture ne fonctionne que dans la mesure où une attention exclusive est accordée aux facteurs de structuration internes. Elle perd toute pertinence dès l’instant où sont prises en compte les contraintes externes imposées par le processus d’élargissement de l’Union européenne. Les partis politiques d’Europe centrale et orientale doivent se positionner face aux exigences de la Commission. Il leur faut par ailleurs relayer les intérêts que ces mêmes exigences confortent ou contrarient au sein de la population. La nécessité d’articuler les sollicitations externes et internes préside à une connexion indirecte des dynamiques politique et sociale.

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18

Stasiak, Maciej. "Blocage interne point à point dans les réseaux de connexion." Annales Des Télécommunications 43, no. 9-10 (September 1988): 561–75. http://dx.doi.org/10.1007/bf03011113.

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19

Morel, Pierre-Marie. "Du De motu à la Génération des animaux. Une connexion oubliée du corpus aristotélicien." Anais de Filosofia Clássica 12, no. 24 (December 12, 2018): 1–17. http://dx.doi.org/10.47661/afcl.v12i24.25974.

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À première vue, la Génération des animauxet le Mouvement des animauxne se rencontrent que de manière ponctuelle et leur convergence semble être accidentelle. Cependant, une analyse plus poussée montre qu’ils sont étroitement liés. Trois éléments méritent d’être considérés de plus près. (1) Les dernières lignes du MAannoncent le projet de la GAet la présentent comme la partie suivante d’un programme scientifique commun. (2) Les deux traités ont recours au modèle mécanique des automates, afin d’expliquer les processus dynamiques qu’ils abordent (dans le MA : la modification spontanée des parties qui explique le mouvement local ; dans le GA : la puissance persistante du moteur de la génération durant le processus génératif). (3) Dans les deux cas, la relation agent-patient joue un rôle fondamental et elle est exposée en des termes très semblables. En conclusion, sans formuler d’hypothèse sur l’ordre chronologique des deux traités, on peut supposer que le MA(au moins en partie) et la GAappartiennent au même programme : l’explication des mouvements internes (ou organiques) de l’animal, et plus particulièrement l’analyse de la relation entre moteur et mû. Il apparaît enfin que les deux traités ont en commun une conception de l’unité organique qui n’est pas mise en péril par la distinction interne entre ce meut et ce qui est mû.

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20

Andrieu, Bernard. "La clinique trans-spécique des hybrides : humains ou non-humains ?" psychologie clinique, no. 49 (2020): 114–25. http://dx.doi.org/10.1051/psyc/202049114.

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Les hybrides sont des cyborgs au corps moins double que composé d’une unité osmotique. Par osmose les hybrides se connectent directement à même le corps ou dans le corps même. La quasiprothèse est si incorporée qu’elle devient un quasi-corps à la fois par sa dimension de nouvelle unité fonctionnelle et par l’extension de l’image du corps à une nouvelle apparence de soi. Mais devenue une osmose trans-spécique, l’hybridation forme un seul corps fonctionnel par la connexion interne du corps biologique et de l’écran neurotechnique de l’implant. L’implant est un écran interactif qui recueille des data pour déployer un algorithme.

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Carballo, Alfonso, Daniel Pfenniger, David Carballo, Arnaud Perrier, Francois Mach, Brenda Kwak, Sebastian Carballo, and Anna Pfenniger. "Emerging Roles for Connexins in Hypertension." Current Hypertension Reviews 4, no. 3 (August 1, 2008): 177–82. http://dx.doi.org/10.2174/157340208785132644.

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22

Evans, W. Howard. "Cell communication across gap junctions: a historical perspective and current developments." Biochemical Society Transactions 43, no. 3 (June 1, 2015): 450–59. http://dx.doi.org/10.1042/bst20150056.

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Collaborative communication lies at the centre of multicellular life. Gap junctions (GJs) are surface membrane structures that allow direct communication between cells. They were discovered in the 1960s following the convergence of the detection of low-resistance electrical interactions between cells and anatomical studies of intercellular contact points. GJs purified from liver plasma membranes contained a 27 kDa protein constituent; it was later named Cx32 (connexin 32) after its full sequence was determined by recombinant technology. Identification of Cx43 in heart and later by a further GJ protein, Cx26 followed. Cxs have a tetraspan organization in the membrane and oligomerize during intracellular transit to the plasma membrane; these were shown to be hexameric hemichannels (connexons) that could interact end-to-end to generate GJs at areas of cell-to-cell contact. The structure of the GJ was confirmed and refined by a combination of biochemical and structural approaches. Progress continues towards obtaining higher atomic 3D resolution of the GJ channel. Today, there are 20 and 21 highly conserved members of the Cx family in the human and mouse genomes respectively. Model organisms such as Xenopus oocytes and zebra fish are increasingly used to relate structure to function. Proteins that form similar large pore membrane channels in cells called pannexins have also been identified in chordates. Innexins form GJs in prechordates; these two other proteins, although functionally similar, are very different in amino acid sequence to the Cxs. A time line tracing the historical progression of wide ranging research in GJ biology over 60 years is mapped out. The molecular basis of channel dysfunctions in disease is becoming evident and progress towards addressing Cx channel-dependent pathologies, especially in ischaemia and tissue repair, continues.

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Schubert, Anne-Lane, William Schubert, David C. Spray, and Michael P. Lisanti. "Connexin Family Members Target to Lipid Raft Domains and Interact with Caveolin-1†." Biochemistry 41, no. 18 (May 2002): 5754–64. http://dx.doi.org/10.1021/bi0121656.

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24

Matthews, Dale A. "Making Connexions: Enhancing the Therapeutic Potential of PatientClinician Relationships." Annals of Internal Medicine 118, no. 12 (June 15, 1993): 973. http://dx.doi.org/10.7326/0003-4819-118-12-199306150-00010.

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Johnson, Kristen E., Shalini Mitra, Parul Katoch, Linda S. Kelsey, Keith R. Johnson, and Parmender P. Mehta. "Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells." Molecular Biology of the Cell 24, no. 6 (March 15, 2013): 715–33. http://dx.doi.org/10.1091/mbc.e12-07-0537.

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The molecular mechanisms regulating the assembly of connexins (Cxs) into gap junctions are poorly understood. Using human pancreatic tumor cell lines BxPC3 and Capan-1, which express Cx26 and Cx43, we show that, upon arrival at the cell surface, the assembly of Cx43 is impaired. Connexin43 fails to assemble, because it is internalized by clathrin-mediated endocytosis. Assembly is restored upon expressing a sorting-motif mutant of Cx43, which does not interact with the AP2 complex, and by expressing mutants that cannot be phosphorylated on Ser-279 and Ser-282. The mutants restore assembly by preventing clathrin-mediated endocytosis of Cx43. Our results also document that the sorting-motif mutant is assembled into gap junctions in cells in which the expression of endogenous Cx43 has been knocked down. Remarkably, Cx43 mutants that cannot be phosphorylated on Ser-279 or Ser-282 are assembled into gap junctions only when connexons are composed of Cx43 forms that can be phosphorylated on these serines and forms in which phosphorylation on these serines is abolished. Based on the subcellular fate of Cx43 in single and contacting cells, our results document that the endocytic itinerary of Cx43 is altered upon cell–cell contact, which causes Cx43 to traffic by EEA1-negative endosomes en route to lysosomes. Our results further show that gap-junctional plaques formed of a sorting motif–deficient mutant of Cx43, which is unable to be internalized by the clathrin-mediated pathway, are predominantly endocytosed in the form of annular junctions. Thus the differential phosphorylation of Cx43 on Ser-279 and Ser-282 is fine-tuned to control Cx43’s endocytosis and assembly into gap junctions.

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26

Shakespeare, Teresa I., Caterina Sellitto, Leping Li, Clio Rubinos, Xiaohua Gong, Miduturu Srinivas, and Thomas W. White. "Interaction between Connexin50 and Mitogen-activated Protein Kinase Signaling in Lens Homeostasis." Molecular Biology of the Cell 20, no. 10 (May 15, 2009): 2582–92. http://dx.doi.org/10.1091/mbc.e08-12-1257.

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Both connexins and signal transduction pathways have been independently shown to play critical roles in lens homeostasis, but little is known about potential cooperation between these two intercellular communication systems. To investigate whether growth factor signaling and gap junctional communication interact during the development of lens homeostasis, we examined the effect of mitogen-activated protein kinase (MAPK) signaling on coupling mediated by specific lens connexins by using a combination of in vitro and in vivo assays. Activation of MAPK signaling pathways significantly increased coupling provided by Cx50, but not Cx46, in paired Xenopus laevis oocytes in vitro, as well as between freshly isolated lens cells in vivo. Constitutively active MAPK signaling caused macrophthalmia, cataract, glucose accumulation, vacuole formation in differentiating fibers, and lens rupture in vivo. The specific removal or replacement of Cx50, but not Cx46, ameliorated all five pathological conditions in transgenic mice. These results indicate that MAPK signaling specifically modulates coupling mediated by Cx50 and that gap junctional communication and signal transduction pathways may interact in osmotic regulation during postnatal fiber development.

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Stroemlund, Line Waring, Christa Funch Jensen, Klaus Qvortrup, Mario Delmar, and Morten Schak Nielsen. "Gap junctions–guards of excitability." Biochemical Society Transactions 43, no. 3 (June 1, 2015): 508–12. http://dx.doi.org/10.1042/bst20150059.

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Cardiomyocytes are connected by mechanical and electrical junctions located at the intercalated discs (IDs). Although these structures have long been known, it is becoming increasingly clear that their components interact. This review describes the involvement of the ID in electrical disturbances of the heart and focuses on the role of the gap junctional protein connexin 43 (Cx43). Current evidence shows that Cx43 plays a crucial role in organizing microtubules at the intercalated disc and thereby regulating the trafficking of the cardiac sodium channel NaV1.5 to the membrane.

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28

Ritchot, Gilles. "La valorisation économique de l’espace géographique." Cahiers de géographie du Québec 36, no. 98 (April 12, 2005): 175–214. http://dx.doi.org/10.7202/022265ar.

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Par rapport à la structuration de l'établissement humain, les valeurs économiques sont des facteurs externes de nature quantitative et qui varient de façon continue. Leur production localisée dans l'espace géographique peut faire apparaître des morphologies qualitativement distinctes, urbaines et rurales par exemple. L'espace géographique s'impose alors comme étant catégorisé en domaines qualitativement différenciés par un système de discontinuités. De cette connexion entre les valorisations économiques et l'apparaître morphologique de la géographie humaine, il est impossible d'inférer une relation de cause à effet de type réductionniste. Un facteur externe variant de façon continue ne peut pas expliquer une différenciation de formes d'établissement humain qui relève de discontinuités qualitatives. Ces morphologies géographiques sont plutôt engendrées par une dynamique interne irréductible aux valorisations économiques. Cette « morphodynamique » procède de trajectoires de mobilité conditionnées par un interdit universel de propriété qui actualise spatialement des représentations symboliques.

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Locke, Darren, Fabien Kieken, Liang Tao, Paul L. Sorgen, and Andrew L. Harris. "Mechanism for modulation of gating of connexin26-containing channels by taurine." Journal of General Physiology 138, no. 3 (August 15, 2011): 321–39. http://dx.doi.org/10.1085/jgp.201110634.

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The mechanisms of action of endogenous modulatory ligands of connexin channels are largely unknown. Previous work showed that protonated aminosulfonates (AS), notably taurine, directly and reversibly inhibit homomeric and heteromeric channels that contain Cx26, a widely distributed connexin, but not homomeric Cx32 channels. The present study investigated the molecular mechanisms of connexin channel modulation by taurine, using hemichannels and junctional channels composed of Cx26 (homomeric) and Cx26/Cx32 (heteromeric). The addition of a 28–amino acid “tag” to the carboxyl-terminal domain (CT) of Cx26 (Cx26T) eliminated taurine sensitivity of homomeric and heteromeric hemichannels in cells and liposomes. Cleavage of all but four residues of the tag (Cx26Tc) resulted in taurine-induced pore narrowing in homomeric hemichannels, and restored taurine inhibition of heteromeric hemichannels (Cx26Tc/Cx32). Taurine actions on junctional channels were fully consistent with those on hemichannels. Taurine-induced inhibition of Cx26/Cx32T and nontagged Cx26 junctional channels was blocked by extracellular HEPES, a blocker of the taurine transporter, confirming that the taurine-sensitive site of Cx26 is cytoplasmic. Nuclear magnetic resonance of peptides corresponding to Cx26 cytoplasmic domains showed that taurine binds to the cytoplasmic loop (CL) and not the CT, and that the CT and CL directly interact. ELISA showed that taurine disrupts a pH-dependent interaction between the CT and the CT-proximal half of the CL. These studies reveal that AS disrupt a pH-driven cytoplasmic interdomain interaction in Cx26-containing channels, causing closure, and that the Cx26CT has a modulatory role in Cx26 function.

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30

Suchman, Anthony L. "Correction: The Connexional Dimension of Medical Care." Annals of Internal Medicine 109, no. 2 (July 15, 1988): 173. http://dx.doi.org/10.7326/0003-4819-109-2-173_2.

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31

Schmidt-Jones, Catherine Anne. "An open education resource supports a diversity of inquiry-based learning." International Review of Research in Open and Distributed Learning 13, no. 1 (January 31, 2012): 1. http://dx.doi.org/10.19173/irrodl.v13i1.1141.

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There have been numerous calls for research that demonstrates how open education resources (OERs) are actually being used. This case study sought to shed light on the users of a well-visited set of modular music-education materials published at Connexions. Respondents to a voluntary survey included teachers, students, self-directed learners, music ensemble participants, and casual learners. Most reported accessing individual modules on their own initiative, as part of a specific, immediate inquiry, rather than responding to institutional directives or following entire online courses. This was supported by computer-log records, which showed that most visitors to a module arrived from an Internet search for terms specific to that module. The study suggests that, for teachers and students as well as self-directed learners, one function of OERs is as a resource for just-in-time, inquiry-based learning.

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32

Morabito, Caterina, Nathalie Steimberg, Francesca Rovetta, Jennifer Boniotti, Simone Guarnieri, Giovanna Mazzoleni, and Maria A. Mariggiò. "Extremely Low-Frequency Electromagnetic Fields Affect Myogenic Processes in C2C12 Myoblasts: Role of Gap-Junction-Mediated Intercellular Communication." BioMed Research International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/2460215.

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Extremely low-frequency electromagnetic fields (ELF-EMFs) can interact with biological systems. Although they are successfully used as therapeutic agents in physiatrics and rehabilitative practice, they might represent environmental pollutants and pose a risk to human health. Due to the lack of evidence of their mechanism of action, the effects of ELF-EMFs on differentiation processes in skeletal muscle were investigated. C2C12 myoblasts were exposed to ELF-EMFs generated by a solenoid. The effects of ELF-EMFs on cell viability and on growth and differentiation rates were studied using colorimetric and vital dye assays, cytomorphology, and molecular analysis of MyoD and myogenin expression, respectively. The establishment of functional gap junctions was investigated analyzing connexin 43 expression levels and measuring cell permeability, using microinjection/dye-transfer assays. The ELF-EMFs did not affect C2C12 myoblast viability or proliferation rate. Conversely, at ELF-EMF intensity in the mT range, the myogenic process was accelerated, through increased expression of MyoD, myogenin, and connexin 43. The increase in gap-junction function suggests promoting cell fusion and myotube differentiation. These data provide the first evidence of the mechanism through which ELF-EMFs may provide therapeutic benefits and can resolve, at least in part, some conditions of muscle dysfunction.

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33

Catarino, Steve, Teresa M. Ribeiro-Rodrigues, Rita Sá Ferreira, José Ramalho, Christine Abert, Sascha Martens, and Henrique Girão. "A Conserved LIR Motif in Connexins Mediates Ubiquitin-Independent Binding to LC3/GABARAP Proteins." Cells 9, no. 4 (April 7, 2020): 902. http://dx.doi.org/10.3390/cells9040902.

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Gap junctions (GJ) are specialized cell-cell contacts formed by connexins (Cxs), which provide direct communication between adjacent cells. Cx43 ubiquitination has been suggested to induce the internalization of GJs, as well as the recruitment of the autophagy receptor p62 to mediate binding to LC3B and degradation by macroautophagy. In this report, we describe a functional LC3 interacting region (LIR), present in the amino terminal of most Cx protein family members, which can mediate the autophagy degradation of Cx43 without the need of ubiquitin. Mutation of the LIR motif on Cx37, Cx43, Cx46 and Cx50 impairs interaction with LC3B and GABARAP without compromising protein ubiquitination. Through in vitro protein-protein interaction assays, we demonstrate that this LIR motif is required for the binding of Cx43 to LC3B and GABARAP. Overall, our findings describe an alternative mechanism whereby Cxs interact with LC3/GABARAP proteins, envisioning a new model for the autophagy degradation of connexins.

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34

Wang, C. M., J. Lincoln, J. E. Cook, and D. L. Becker. "Abnormal Connexin Expression Underlies Delayed Wound Healing in Diabetic Skin." Diabetes 56, no. 11 (August 23, 2007): 2809–17. http://dx.doi.org/10.2337/db07-0613.

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35

Meda, P. "The In Vivo -to- -Cell Chat Room: Connexin Connections Matter." Diabetes 61, no. 7 (June 21, 2012): 1656–58. http://dx.doi.org/10.2337/db12-0336.

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36

Boerma, M., L. Forsberg, L. Van Zeijl, R. Morgenstern, U. De Faire, C. Lemne, D. Erlinge, T. Thulin, Y. Hong, and I. A. Cotgreave. "A genetic polymorphism in connexin 37 as a prognostic marker for atherosclerotic plaque development." Journal of Internal Medicine 246, no. 2 (August 1999): 211–18. http://dx.doi.org/10.1046/j.1365-2796.1999.00564.x.

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37

WANG, L., J. CHEN, Y. SUN, F. ZHANG, J. ZHU, S. HU, and D. WANG. "Regulation of Connexin Expression After Balloon Injury: Possible Mechanisms for Antiproliferative Effect of Statins." American Journal of Hypertension 18, no. 9 (September 2005): 1146–53. http://dx.doi.org/10.1016/j.amjhyper.2005.03.746.

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38

Morschauser, Timothy J., Jayanth Ramadoss, Jill M. Koch, Fu Xian Yi, Gladys E. Lopez, Ian M. Bird, and Ronald R. Magness. "Local Effects of Pregnancy on Connexin Proteins That Mediate Ca 2+ -Associated Uterine Endothelial NO Synthesis." Hypertension 63, no. 3 (March 2014): 589–94. http://dx.doi.org/10.1161/hypertensionaha.113.01171.

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39

Le Gal, Loïc, Florian Alonso, Charlotte Wagner, Stéphane Germain, Denise Nardelli Haefliger, Paolo Meda, and Jacques-Antoine Haefliger. "Restoration of Connexin 40 (Cx40) in Renin-Producing Cells Reduces the Hypertension of Cx40 Null Mice." Hypertension 63, no. 6 (June 2014): 1198–204. http://dx.doi.org/10.1161/hypertensionaha.113.02976.

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40

Charpantier, E., J. Cancela, and P. Meda. "Beta cells preferentially exchange cationic molecules via connexin 36 gap junction channels." Diabetologia 50, no. 11 (September 8, 2007): 2332–41. http://dx.doi.org/10.1007/s00125-007-0807-9.

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41

Cigliola, Valentina, Florent Allagnat, Lukas Adrian Berchtold, Smaragda Lamprianou, Jacques-Antoine Haefliger, and Paolo Meda. "Role of Connexins and Pannexins in the Pancreas." Pancreas 44, no. 8 (November 2015): 1234–44. http://dx.doi.org/10.1097/mpa.0000000000000378.

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42

Masamune, Atsushi, Noriaki Suzuki, Kazuhiro Kikuta, Hiroyuki Ariga, Shintaro Hayashi, Tetsuya Takikawa, Kiyoshi Kume, et al. "Connexins Regulate Cell Functions in Pancreatic Stellate Cells." Pancreas 42, no. 2 (March 2013): 308–16. http://dx.doi.org/10.1097/mpa.0b013e31825c51d6.

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43

Umans, Jason G., Marshall D. Lindheimer, Bradley Hack, and Cathleen A. Davidson-Garcia. "Connexin Expression is not Altered in Omental Resistance Vessels from Women with Preeclampsia." Hypertension in Pregnancy 20, no. 1 (January 2001): 119–24. http://dx.doi.org/10.3109/10641950109152648.

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44

Meilleur, Mélissa-Anne, Casimir D. Akpovi, R. Marc Pelletier та María Leiza Vitale. "Tumor Necrosis Factor-α-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation". Endocrinology 148, № 12 (1 грудня 2007): 5913–24. http://dx.doi.org/10.1210/en.2007-0767.

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The anterior pituitary folliculostellate (FS) cells are key elements of the paracrine control of the pituitary function. These cells are the source and the target of growth factors and cytokines, and are connected to other pituitary cells via Cx43-mediated gap junctions. Here, we show that acute treatment of the FS TtT/GF cell line with TNF-α caused a transient cell uncoupling that was accompanied by the dephosphorylation of Cx43 in Ser368. These TNF-α-evoked effects were dependent on protein phosphatase 2A (PP2A) and protein kinase C (PKC) activities. TNF-α did not affect total cell Cx43-PP2A catalytic subunit interaction, but it did induce PP2A catalytic subunit recruitment to the Triton X-100 insoluble subcellular fraction, in which Cx43-gap junction plaques are recovered. This recruitment temporally coincided with Cx43 phosphorylated in Ser368-Cx43 dephosphorylation. Cx43 did not interact with the conventional PKC-α, but it did interact with the atypical PKC-ζ. Moreover, this interaction was weakened by TNF-α. Cx43 dephosphorylation in Ser368 was followed by the tyrosine phosphorylation of the protein. The temporary closure of gap junctions during acute TNF-α challenge may constitute a protective mechanism to limit or confine the spread of inflammatory signals among the FS cells.

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45

Rummery, Nicole M., Katja U. S. McKenzie, Judith A. Whitworth, and Caryl E. Hill. "Decreased endothelial size and connexin expression in rat caudal arteries during hypertension." Journal of Hypertension 20, no. 2 (February 2002): 247–53. http://dx.doi.org/10.1097/00004872-200202000-00014.

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46

Culler, C. E., P. S. Gargalovic, T. G. Kirchgessner, and A. J. Lusis. "Tu-P7: 156 Regulation of connexin 37 by oxidized phospholipids in atherosclerosis." Atherosclerosis Supplements 7, no. 3 (January 2006): 219. http://dx.doi.org/10.1016/s1567-5688(06)80862-7.

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47

Ahn, Meejin, Jonathan Lee, Andreas Gustafsson, Alan Enriquez, Eric Lancaster, Jai-Yoon Sul, Philip G. Haydon, et al. "Cx29 and Cx32, two connexins expressed by myelinating glia, do not interact and are functionally distinct." Journal of Neuroscience Research 86, no. 5 (April 2008): 992–1006. http://dx.doi.org/10.1002/jnr.21561.

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48

SATO, SATSUKI, JINYA SUZUKI, MASAMICHI HIROSE, TAKAHIRO NAKAYA, MIKA YAMADA, MAI ICHIKAWA, MICHIKO IMAGAWA, et al. "Overexpression of Perilipin 2 Induces Cardiac Steatosis and Atrial Fibrillation via Connexin 43 Remodeling." Diabetes 67, Supplement 1 (May 2018): 1903—P. http://dx.doi.org/10.2337/db18-1903-p.

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49

Mozafari, Sabah, Laura Starost, Blandine Manot-Saillet, Beatriz Garcia-Diaz, Yu Kang T. Xu, Delphine Roussel, Marion J. F. Levy, et al. "Multiple sclerosis iPS-derived oligodendroglia conserve their properties to functionally interact with axons and glia in vivo." Science Advances 6, no. 49 (December 2020): eabc6983. http://dx.doi.org/10.1126/sciadv.abc6983.

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Remyelination failure in multiple sclerosis (MS) is associated with a migration/differentiation block of oligodendroglia. The reason for this block is highly debated. It could result from disease-related extrinsic or intrinsic regulators in oligodendroglial biology. To avoid confounding immune-mediated extrinsic effect, we used an immune-deficient mouse model to compare induced pluripotent stem cell–derived oligodendroglia from MS and healthy donors following engraftment in the developing CNS. We show that the MS-progeny behaves and differentiates into oligodendrocytes to the same extent as controls. They generate equal amounts of myelin, with bona fide nodes of Ranvier, and promote equal restoration of their host slow conduction. MS-progeny expressed oligodendrocyte- and astrocyte-specific connexins and established functional connections with donor and host glia. Thus, MS oligodendroglia, regardless of major immune manipulators, are intrinsically capable of myelination and making functional axo-glia/glia-glia connections, reinforcing the view that the MS oligodendrocyte differentiation block is not from major intrinsic oligodendroglial deficits.

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Girard, M., and D. Malauzat. "Aperçu des moyens de communications utilisés par les usagers d’un centre hospitalier psychiatrique français : fracture numérique ou sociale ?" European Psychiatry 29, S3 (November 2014): 544. http://dx.doi.org/10.1016/j.eurpsy.2014.09.326.

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La place des technologies de communication grandit dans le quotidien, et l’organisation du soin en médecine. Afin d’en évaluer l’accès et l’usage, nous avons diffusé un questionnaire auprès des personnes hospitalisées au Centre Hospitalier Esquirol de Limoges, hôpital public de soin en psychiatrie à vocation régionale. Notre intérêt a porté sur le type de moyens de communication dont les personnes disposent, incluant les moyens de transport, et leur fréquence d’usage. Le questionnaire, anonyme, sans collecte de donnée médicale ou identifiante, a été diffusé auprès des patients de plus de 12 ans, accueillis en hospitalisation complète ou partielle durant la semaine du 7 au 11 avril 2014.Les 954 questionnaires (par rapport aux 1044 théoriques) exploités correspondent à une population représentative en âge et en sexe de celle alors hospitalisées dans l’établissement. Les résultats montrent un plus faible équipement en support de communication (65 % ont un téléphone portable), et des connexions à Internet moins fréquentes qu’en population générale (54 % ne se connectent jamais). Concernant les lieux et moyens de consultation Internet, l’ordinateur personnel est le plus cité, mais pour 34 % seulement, l’usage du téléphone portable restant peu répandu. La voiture reste le moyen de transport majoritaire mais pour 60 % seulement. L’accès et l’usage des moyens de communications actuels sont plus réduits qu’en population générale [1,2], même en tenant compte de l’influence de l’âge et de la ruralité. Enfin, les moins connectés et les moins utilisateurs de technologies sont les personnes en hospitalisation partielle, âgées, accueillis en filières de soin des troubles psychotiques.Ainsi, la possibilité d’intégrer ces techniques dans le projet individualisé de soin (rappel de rendez-vous, d’examens…) nécessite au préalable l’équipement et/ou sa mise à jour pour atteindre les populations les plus concernées.

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