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Добірка наукової літератури з теми "CONGO-RED STAINING"

Автор: Grafiati

Опубліковано: 11 вересня 2023

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Статті в журналах з теми "CONGO-RED STAINING"

Emmett, Ern. "Microscopical techniques: staining with Congo Red." Field Mycology 4, no. 2 (April 2003): 72. http://dx.doi.org/10.1016/s1468-1641(10)60194-6.

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Kibby, Geoffrey. "Staining cap cuticle with congo red dye." Field Mycology 7, no. 1 (January 2006): 36. http://dx.doi.org/10.1016/s1468-1641(10)60359-3.

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Guselnikova, Valeriia V., Elena A. Fedorova, Alexandra Ja Gudkova, Michael M. Shavlovsky, and Dmitrii E. Korzhevskii. "Transthyretin amyloid cardiomyopathy. Features of histological diagnosis: study design." Terapevticheskii arkhiv 94, no. 4 (May 26, 2022): 473–78. http://dx.doi.org/10.26442/00403660.2022.04.201464.

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Анотація:

Aim. To compare efficiency and specific features of transthyretin amyloid staining by different histological dyes and thus to assess their suitability for diagnostic purposes. Materials and methods. Samples of left and right heart ventricles were taken from patients over 70 years-old of both genders (n=10) with immunohistochemically verified transthyretin amyloidosis (ATTR). All samples were stained with Congo red, Alcian blue, toluidine blue and methylene violet. Results. Specificity and sensitivity of Congo red staining was comparable to those of immunohistochemical staining. For verification of amyloid presence after Congo red staining one could use fluorescent microscopy instead of polarization microscopy. It allows a more accurate diagnosis of amyloidosis. Confocal microscopy with spectral unmixing improves detection sensitivity of amyloid by elimination of background fluorescence of muscle tissue and autofluorescence of lipofuscin. Alcian blue staining gives the same result as Congo red. In addition, its less labor-intensive and free of false-positive and false-negative results caused by final processing of slide preparation. Toluidine blue and methylene violet develop metachromatic staining upon binding to transthyretin fibrils, likely due to specific biochemical features of these fibrils. Conclusion. The most reliable method for histochemical diagnosis of ATTR is the Congo red staining with subsequent analysis using fluorescence or confocal microscopy. For diagnostic screening, the use of Sodium sulphate-Alcian blue staining method is highly promising. Metachromatic stains are less effective for ATTR diagnosis.

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NORN, M. S. "CONGO RED VITAL STAINING OF CORNEA AND CONJUNCTIVA." Acta Ophthalmologica 54, no. 5 (May 27, 2009): 601–10. http://dx.doi.org/10.1111/j.1755-3768.1976.tb01289.x.

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Kadota, Ayumi, Susumu Iwaide, Shinya Miyazaki, Ikki Mitsui, Noboru Machida, and Tomoaki Murakami. "Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats." Veterinary Pathology 57, no. 5 (June 17, 2020): 658–65. http://dx.doi.org/10.1177/0300985820934113.

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Анотація:

Amyloidosis is classified according to the amyloid precursor protein, and accurate diagnosis of the amyloidosis type may guide appropriate treatment. Immunohistochemistry and Congo red staining are the most frequently used methods used to distinguish types of amyloidosis, but problems with specificity and sensitivity indicate the need for an alternative diagnostic method. In this study, we evaluated laser microdissection-liquid chromatography-tandem mass spectrometry (LMD-LC-MS/MS) for the diagnosis of amyloid light-chain (AL) amyloidosis in animals. Plasmacytomas with amyloid deposits from 15 dogs and 2 cats were subjected to Congo red staining with or without potassium permanganate pretreatment, immunohistochemistry for kappa and lambda light chains, and LMD-LC-MS/MS. Congo red staining was diagnostic in 12 of 17 cases based on resistance to potassium permanganate pretreatment, but in 5 of 17 cases the pretreatment unexpectedly reduced Congo red staining or abrogated the birefringence and a definitive diagnosis could not be reached. Immunohistochemistry detected kappa or lambda light chains in 6 of 17 cases. With LMD-LC-MS/MS, immunoglobulin lambda light chain was detected in all 17 cases. The amyloid signature proteins ApoA-I, ApoA-IV, and ApoE were detected in 9, 1, and 3 of the 15 canine cases by LMD-LC-MS/MS, but not in the feline cases. In conclusion, LMD-LC-MS/MS consistently determined the amyloid type in all examined specimens, while Congo red staining after potassium permanganate treatment and immunohistochemistry were less sensitive tests.

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Schulze, C., M. Brügmann, M. Böer, H. P. Brandt, J. Pohlenz, and R. P. Linke. "Generalized AA-amyloidosis in Siberian Tigers (Panthera tigris altaica) with Predominant Renal Medullary Amyloid Deposition." Veterinary Pathology 35, no. 1 (January 1998): 70–74. http://dx.doi.org/10.1177/030098589803500108.

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Анотація:

Generalized amyloidosis with predominant renal medullary amyloid deposition was found in four closely related Siberian tigers ( Panthera tigris altaica) suffering from end stage kidney diseases. Only minimal to mild amounts of amyloid were deposited in various organs outside the kidneys with individually variable organ involvement. The Congo red staining affinity of amyloid deposits was sensitive to potassium permanganate oxidation. The deposits were further characterized as being of the amyloid-A (AA) type by immunohistochemistry using the mouse monoclonal antibody mc4 directed against a conserved region of the human AA-protein. A combination of immunohistochemistry and Congo red staining was much more sensitive for the diagnosis of amyloid deposits than Congo red staining alone. With this combination, even minimal amyloid deposits were detected that had been missed in the first reading using Congo-red-stained slides alone. Since no common primary cause was identified, the amyloidosis was classified as idiopathic generalized AA-amyloidosis with a potential familial predisposition.

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Gruber, A. D., and R. P. Linke. "Generalized AA-Amyloidosis in a Bat (Pipistrellus pipistrellus)." Veterinary Pathology 33, no. 4 (July 1996): 428–30. http://dx.doi.org/10.1177/030098589603300409.

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Generalized amyloidosis was found to be the cause of death in a female adult insectivorous pipistrelle bat ( Pipistrellus pipistrellus) after chronic wound inflammation. Large amounts of amyloid were detected in liver, spleen, kidneys, stomach, intestine, lymphatic tissues, and endocrine and salivary glands. Congo red staining and green birefringence identified amyloid; the Congo red staining was sensitive to potassium permanganate oxidation. The amyloid was further classified immunohistochemically. The deposits reacted with two anti-human-AA-amyloid monoclonal antibodies in a peroxidase-antiperoxidase reaction, whereas no reaction was found with antibodies specific for other types of amyloid. Thus, the bat amyloid deposits were identified as generalized reactive AA-amyloidosis.

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Bély, M. "Histochemical Differential Diagnosis and Polarization Optical Analysis of Amyloid and Amyloidosis." Scientific World JOURNAL 6 (2006): 154–68. http://dx.doi.org/10.1100/tsw.2006.35.

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Анотація:

Amyloidosis is characterized by extracellular deposition of protein fibrils of chemically heterogeneous composition. Early recognition and identification of amyloid deposits allows an early start of therapy, which may entail a better prognosis. Congo red staining according to Romhányi (1971) is a highly specific and sensitive method for early microscopic recognition of amyloidosis. The main and most important types of amyloidosis may be distinguished by classic histochemical methods of performate pretreatment according to Romhányi (1979), or by KMnO4oxidation according to Wright (1977) followed by Congo red staining and viewed under polarized light. Differences in the speed of breakdown (disintegration) of amyloid deposits according to Bély and Apáthy allow a more precise distinction of various types of amyloid.

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Hossain, Bassam, and Sumair Ahmad. "S2605 GI Amyloidosis: A Case of Congo Red Staining and Anemia." American Journal of Gastroenterology 117, no. 10S (October 2022): e1726-e1726. http://dx.doi.org/10.14309/01.ajg.0000867060.62730.83.

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Bennett, J. D. C., and C. R. Chowdhury. "Primary amyloidosis of the larynx." Journal of Laryngology & Otology 108, no. 4 (April 1994): 339–40. http://dx.doi.org/10.1017/s0022215100126702.

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AbstractThe history, examination and operative findings of primary amyloidosis of the larynx are very suggestive of carcinoma, indicating the need for careful histological examination. Staining with Congo red shows a characteristic birefringence. Systemic amyloidosis may be present

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Більше джерел

Дисертації з теми "CONGO-RED STAINING"

Östlund, Helena. "Evaluating the Congo red staining method with the aim to solve problematics in the work process and optimize amyloidosis diagnostics." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-327122.

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Анотація:

Some diagnostic methods have been used for a very long time. Congo red stain saw the light of day in 1883, and quickly became important in many fields of use. Nowadays we recognize the importance of Congo red in diagnose of amyloid diseases. However, the technique and experience needed throughout the process from a suspected case to the diagnose is of greate importance. When diagnostic difficulties appeared in a few patient cases at the local hospital in Gävle, Sweden, a solution was needed. A delayed diagnose could have a potential devastating outcome seen in the perspective of the patient. Therefore it is crucial to have both sensitive and specific diagnostic methods that are optimized against the sought pathogenesis. This study aspired to find the solution to the difficulties in diagnostic work, brought to light by a pathology doctor at the hospital. Several different methodical procedures are used throughout the process, and were evaluated with focus lying on the thickness of the tissue, the staining method and the microscopes used in diagnostics. Different thickness of the tissue was cut and stained. The results demonstrated the importance of proper techniques and methods in preparing the tissue, and the tools to analyse it with. The thickness of tissue and the lightsource in the microscope played a cruicial role in diagnostics. Additionally it showed the importance to continue to raise the quality of work and make progress in the diagnostic and scientific field, possibly by finding new applications for old methods.

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JOSHI, KHYATI. "SCREENING, ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM ENVIRONMENT." Thesis, 2022. http://dspace.dtu.ac.in:8080/jspui/handle/repository/19163.

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Lignocellulosic biomass is the most plentiful matter on the earth. It holds potential to be transformed into value added chemicals like bioethanol, organic acid, bioplastics, enzymes, pigments etc. Lignocellulosic biomass is composed of cellulose, hemicellulose and lignin. Cellulose is the most prevalent organic matter on the planet and is a key glucose storage type. The fundamental step in the bioconversion of LCB into value-added bioproducts is based on the decomposition of cellulose by cellulase. Various groups of microbes including fungi and bacteria are known for their ability to produce cellulase. Although fungi have the most efficient cellulase activity, bacterial cellulase is gaining popularity because of its higher growth rate, better specific activity, stability and mass transfer. Therefore, identification of a novel and better bacterial strain with higher cellulase productivity and enhanced activity could be a breakthrough in the utilization of lignocellulosic biomass for various applications. In this study, cellulase producing bacteria were screened and isolated from soil sample. They were stained and qualitatively analysed for their cellulolytic activity using Congo-red staining. The bacteria showing maximum clear zone was used for further work. Its growth curve was analysed and its enzymatic activity was measured quantitatively using different assays. It was observed that maximum cell biomass was produced after 16 hours of incubation whereas maximum enzymatic activity of 0.4 IU/mL was observed after 14 hours of incubation.

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Книги з теми "CONGO-RED STAINING"

Cohen, Jeffrey A., Justin J. Mowchun, Victoria H. Lawson, and Nathaniel M. Robbins. A 44-Year-Old Male with Subacute Onset of Syncope. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190491901.003.0032.

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Syncope in a patient with orthostatic hypotension (OH) may indicate autonomic dysfunction. The definition of OH is presented. Clinical features of parasympathetic and sympathetic function are discussed. The differential of acute autonomic dysfunction includes a number of conditions. An autoimmune etiology may occur autoimmune autonomic ganglionopathy. Serologic testing can assist in this diagnosis. If autoimmune immune modulating therapies may be indicated. Autonomic neuropathy may be a paraneoplastuc syndrome. Autonomic testing can also help with documenting autonomic neuropathy as well as the whether the defects are predominately parasympathetic or sympathetic. Amyloid should be considered as should diabetes but both have a more chronic course. An appropriate tissue biopsy with Congo Red staining can help to confirm the diagnosis of amyloid.

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Частини книг з теми "CONGO-RED STAINING"

Juneja, Richa, Prasad Dange, and Rahul Arora. "Diagnosis of Amyloidosis: From History to Current Tools." In Amyloidosis - History and Perspectives. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.102006.

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Анотація:

The term amyloid encompasses a large variety of misfolded proteins with varying amino acids unified by the antiparallel beta-pleated sheet configuration and characteristic Congo red staining. The etiology of these proteins is equally varied, ranging from neoplastic plasma cell disorder, hereditary causes to inflammatory disorders. The protean clinical manifestation makes a high index of clinical suspicion the first crucial step in the diagnosis. A battery of investigations needs to be carried out for a complete diagnosis of amyloidosis and its underlying etiology. Biopsy with Congo red staining constitutes the most important modality for confirmation of amyloid. For further testing, varying modalities with increasing complexity, such as immunohistochemistry, electron microscopy, and mass spectrometry, need to be employed. We discuss, in the course of the current chapter, this fascinating protein from a clinical diagnosis perspective. The requisite investigations are also discussed in detail.

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Тези доповідей конференцій з теми "CONGO-RED STAINING"

Souza, Carlos Dornels Freire de, Lucas Gomes Santos, João Paulo Silva de Paiva, Thiago Cavalcanti Leal, Gibson Barros de Almeida Santana, Thais Silva Matos, Carlos Alberto de Oliveira Rocha, et al. "Pure neural leprosy or amyloid neuropathy? systematic review and clinical case report." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.227.

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Анотація:

Objective: To review the literature, including a clinical case discussion with suspicion of pure neural leprosy and final diagnosis of amyloid neuropathy. Methods: The study was conducted on May 28, 2020. A systematic review of the literature was conducted, with searches in PubMed, Medline, Lilacs and BVS MS using the descriptors: neuritic leprosy, pure neural leprosy, primary neural leprosy, pure neuritic leprosy, amyloid polyneuropathy, amyloid neuropathies, amyloid polyneuropathy. Clinical trials, cohorts, cross-sectional, clinical cases and case studies, published in Portuguese, English or Spanish between 2010 and 2020 were included. Then, a case report with an initial suspicion of pure neural leprosy was presented. Laboratory tests, electroneuromyography, ultrasound and biopsy of the sural nerve were requested. Results: 23 scientific texts were included. No publications with the two themes together were found. Diagnosis of pure neural leprosy and the possibility of using auxiliary resources were the most prominent themes in the studies. In the clinical case, the patient’s electroneuromyography showed sensitive and motor polyneuropathy of lower limbs, symmetrical, of moderate intensity, of the mixed type (axono- demyelinating), sensitive and axonal. Ultrasonography of the sural nerve revealed changes in the contour of the deep fibular nerves and biopsy of the sural nerve demonstrated the accumulation of amorphous eosinophilic material in the nerve path and Congo red staining showed birefringence of the deposit in applegreen under polarized light. The final diagnosis was amyloidotic neuropathy. Conclusion: Clinical diagnosis was amyloidotic neuropathy. Diagnosis of pure neural leprosy in endemic areas of Brazil is a challenge for the health system.

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