Статті в журналах: "Coagulase-positive staphylococci"

1

Katic, Vera. "Significance of coagulase-positive staphylococci for microbiological food safety." Veterinarski glasnik 62, no. 5-6 (2008): 275–87. http://dx.doi.org/10.2298/vetgl0806275k.

Повний текст джерела

Анотація:

Staphylococcal foodborne intoxication, in which major symptoms are vomiting and diarrhoea, occurs after ingestion of thermostable staphylococcal enterotoxins produced in food by enterotoxigenic strains of coagulase-positive staphylococci. Staphylococcal enterotoxins are normally not or only slightly inactivated during food processing, storage, distribution or during the preparation of the food in the kitchen. Therefore, if enterotoxigenic staphylococci are able to grow in food to more then 105 - 106 cfu/g/ml before they are killed there is still a risk of intoxication with consumption. The legislation of the Republic of Serbia lays down criteria for coagulase- positive staphylococci in food. However, the number of coagulase-positive staphylococci may not always be a good indicator of the presence of staphylococal enterotoxins, and the number of cells may have already decreased although the product still contains enterotoxins. The microbiological criteria for coagulase-positive staphylococci and/or staphylococcal enterotoxins in food are essential and useful to protect public health.

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Sleiniute, Jurate, and Jurate Siugzdaite. "Distribution of coagulase-positive staphylococci in humans and dogs." Acta Veterinaria Brno 84, no. 4 (2015): 313–20. http://dx.doi.org/10.2754/avb201584040313.

Повний текст джерела

Анотація:

Staphylococci form part of the normal flora of humans and a wide variety of animals. Some staphylococcal species also cause infections for human and household pets. The aim of this study was to investigate the prevalence of coagulase-positive staphylococci in humans and dogs within a common household. Coagulase-positive staphylococci could not be identified phenotypically in all cases. Molecular methods were used for the correct identification of pathogenic staphylococci. The prevalence of Staphylococcus aureus was identified in 26/72 (36.1%) dog owners and in 4/61 (6.6%) dogs. Staphylococcus pseudintermedius was isolated from three (4.2%) humans, and from 28 dogs (45.9%). Other coagulase-positive staphylococci species were not isolated in this study. Colonization of S. aureus in humans was significantly higher than in dogs (P < 0.001). Staphylococcus pseudintermedius was more common in dogs compared to humans (P < 0.001). Staphylococcus pseudintermedius strains were isolated from dogs with clinical symptoms more frequently compared to healthy dogs (P = 0.02). Colonization of S. aureus in female dogs was significantly higher compared to male dogs (P = 0.045). The same staphylococci species were isolated from the dog and its owner in six households (12%, 6/50).

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Coimbra-e-Souza, V., M. A. V. P. Brito, R. C. Chamon, M. S. Laport, and M. Giambiagi-deMarval. "Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, no. 6 (November 2017): 1669–75. http://dx.doi.org/10.1590/1678-4162-9351.

Повний текст джерела

Анотація:

ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and coagulase-positive Staphylococcus aureus (2). Our results strongly support the need to identify the isolates to a species level in order to avoid misidentification and to be aware of the classification using the coagulase test alone.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

NWOSU, V. C. "Prevalence of Coagulase-Positive Staphylococcus in Market Meats in Awka." Journal of Food Protection 48, no. 7 (July 1, 1985): 603–5. http://dx.doi.org/10.4315/0362-028x-48.7.603.

Повний текст джерела

Анотація:

Sixteen samples each of various beef parts including muscle, liver, kidney, heart, and intestines from a local market in Awka, Anambra State of Nigeria were analysed for presence of coagulase-positive Staphylococcus. A total of 80 meat samples was evaluated of which 54 samples or 67.5% had coagulase-positive staphylococci. The percentage of the various meat samples that had coagulase-positive staphylococci was 18.8% for muscle, 75% for liver, 68.8% for kidney, 75% for heart and 100% for intestinal samples. Muscle tissue had the lowest staphylococcal count log (1.65 – 2.97/g), whereas intestinal samples had the highest count (log 5.88 – 6.97g). The organ meats had staphylococcal counts of log 3.10 – 4.89/g for liver samples, log 3.27 – 4.75/g for kidney samples and log 3.15 – 4.90/g for heart samples.

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Aslam, Nadia, Nazma Kiran, and Naima Mehdi. "Frequency of Isolation of Coagulase Negative Staphylococcus from Blood Cultures and its Antibiogram." Journal of Islamabad Medical & Dental College 10, no. 3 (September 30, 2021): 140–44. http://dx.doi.org/10.35787/jimdc.v10i3.468.

Повний текст джерела

Анотація:

Background: Coagulase-negative Staphylococci are members of stable skin inhabitants. They are frequent contaminants in blood cultures and can lead to unnecessary exposure of patients to antimicrobial drugs and excess hospital costs. This study aims to estimate the frequency of Coagulase-negative Staphylococci in blood cultures and their antibiograms. Materials and Methods: This cross-sectional study was performed in a tertiary care hospital over one year from April 2018 to March 2019. Blood cultures received in the laboratory were processed to isolate Coagulase-negative Staphylococci. Susceptibility to various antimicrobial drugs was detected by disc diffusion method and E-strips. Results: A total of 13802 blood cultures were processed in one year. 1750 blood cultures yielded bacterial growth and 374 blood cultures were positive for Staphylococci. Out of these 374 blood cultures, 97 were categorized as Staphylococcus aureus and 277 were Coagulase-negative Staphylococci. Out of 13802 total blood cultures performed during study period, 277 blood cultures positive with Coagulase negative Staphylococcus means contamination rate of 2% out of total blood cultures. 277 Coagulase negative Staphylococcus positive cultures out of 1750 positive blood cultures means contamination rate of 15.8% out of positive blood cultures. Among Coagulase-negative Staphylococci, 68.2% isolates were resistant to Cefoxitin, 95.3% to Penicillin, 85.1% to Erythromycin, 37.5% to Ciprofloxacin, 59.6% to Gentamicin, 68.6% to Fusidic acid, 3.6% to Teichoplanin, and 1.4% to Linezolid. All isolates were sensitive to Vancomycin. Conclusion: The rate of blood culture contamination was 2% out of total blood cultures and 15.8% out of positive blood cultures.

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Radoslava, Savić Radovanović, Zdravković Nemanja, and Velebit Branko. "Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia." Acta Veterinaria 70, no. 2 (June 1, 2020): 238–54. http://dx.doi.org/10.2478/acve-2020-0017.

Повний текст джерела

Анотація:

AbstractA total of 415 cheese samples produced with raw or cooked milk collected from local markets were analysed for the presence of coagulase-positive staphylococci. In 85 (20.48%) samples the presence of coagulase positive staphylococci was detected. The ELFA technique VIDAS SET2 (BioMerieux, France) was used for testing coagulase-positive staphylococci strains to produce classical enterotoxins (SEA, SEB, SEC, SED, SEE), and to determine the enterotoxins in cheese samples. The number of coagulase-positive staphylococci in cheese samples ranged from 1-5.79 log CFU g-1. Out of 85 coagulase-positive strains 26 (30.59%) produced enterotoxins. The presence of genes for the synthesis of staphylococcal enterotoxins (SE) in the obtained extracts of DNA from 26 enterotoxigenic strains was detected by conventional multiplex PCR technique (for genes sea and seb) i.e. the Real-Time PCR technique for genes sec, sed and see. In all 26 strains of coagulase-positive staphylococci (originating from cheeses produced from raw or cooked milk, which were enterotoxin producers) sea was present, and in 24 strains in addition to sea gene, seb was detected. None of the isolates possessed genes for the synthesis of enterotoxin C (SEC), D (SED) and E (SEE). Out of 26 tested cheese samples positive for enterotoxigenic coagulase-positive staphylococci, enterotoxin was detected in 2 (7.69%) samples of sweet-coagulating cheese, in which the number of enterotoxigenic coagulase-positive staphylococci exceeded 5 log CFU g-1. In sweet-coagulating cheeses in which the number of coagulase-positive staphylococci exceeds 5 log CFU g-1 and the pH value was higher than 5.0, enterotoxins may be present in amounts sufficient to cause intoxication.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Boipai, Manju, Krishna Chander Birua, N. P. Sahu, L. B. Pandey, Rameswari Beck, and Shashi Dinkar. "Microbiological and Biochemical Analysis of Methicillin-resistant Staphylococcus aureus Isolated from Patients Admitted in RIMS, Ranchi." Asian Pacific Journal of Health Sciences 7, no. 3 (August 5, 2020): 39–43. http://dx.doi.org/10.21276/apjhs.2020.7.3.10.

Повний текст джерела

Анотація:

Background: Since methicillin-resistant Staphylococcus aureus strains are resistant to multiple antibiotics, there is a possibility of extensive outbreaks which may be difficult to control. Early detection of methicillin-resistant Staphylococcus is important from patients and hospitals point of view. Materials and Methods: The present study was carried out in the Department of Microbiology, Rajendra Institute of Medical Sciences, Ranchi, clinical isolates of methicillin-resistant S. aureus strains were obtained from admitted patients of Rajendra Institute of Medical Sciences, Ranchi. The sources of isolate included pus from infected surgical wounds, infected burn wounds, conjunctival swab, aural swab, throat swab, vaginal swab, and urine for microbiological and biochemical analysis of methicillin-resistant S. aureus. Results: All the 264 cases of staphylococcal species isolated from different clinical specimens were subjected to coagulase test. It was observed that out of 264 strains of staphylococci isolated from different sites, 165 strains (62.5%) were coagulase positive and 99 strains (37.5%) were coagulase negative by tube method. Out of the 165 strains of coagulase-positive staphylococci, maximum isolation was obtained from pus 74 followed by throat swab 55, aural swab 21, vaginal 4, conjunctival swab 9, and urine 2. All the 165 cases of coagulase-positive Staphylococcus isolated from different clinical specimens were studied for hemolysis, mannitol fermentation, pigment production, and phosphatase production. Out of these 165 strains, 162 (98%) strains produced β-hemolysis on blood agar medium. Pigment production was noted in 160 (97%) of cases. Majority of strains produced characteristic golden yellow pigment on nutrient agar plate. A total of 155 (94%) strains of staphylococci fermented mannitol with the production of acid only. Phosphatase production was observed in 157 (95%) strains of pathogenic staphylococci. Conclusion: Considering the above-mentioned pathogenicity test, it was observed that coagulase test was the single most reliable test, though coagulase-negative staphylococci are sometimes pathogenic too.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Horváth, Brigitta, Ferenc Peles, László Bereczki, András Széll, Rita Sipos, Ágnes Erős, Csaba Lovász, and Adrienn Micsinai. "Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS." Acta Agraria Debreceniensis, no. 1 (May 23, 2019): 9–14. http://dx.doi.org/10.34101/actaagrar/1/2362.

Повний текст джерела

Анотація:

Coagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Jankovic, Vesna, Vesna Djordjevic, Brankica Lakicevic, Branka Borovic, B. Velebit, and Radmila Mitrovic. "Determination of staphylococcal enterotoxins in cheese by immunoenzyme assays." Archives of Biological Sciences 64, no. 4 (2012): 1449–54. http://dx.doi.org/10.2298/abs1204449j.

Повний текст джерела

Анотація:

Staphylococcal food poisoning is one of the most common foodborne diseases resulting from the ingestion of staphylococcal enterotoxins (SEs) preformed in foods by enterotoxigenic strains of coagulase-positive staphylococci (CPS), mainly Staphylococcus aureus. The presence of enterotoxigenic strains of coagulase-positive staphylococci in raw milk during the production process leads to the contamination of products and outbreaks of alimentary intoxication. The problem of Staphylococcus aureus in cheese remains significant on a global level. Domestic cheese contaminated with enterotoxigenic staphylococci can result in the formation of enterotoxin, which can produce foodborne illness when the product is ingested. Due to microbiological contamination, microbiological criteria are tools that can be used in assessing the safety and quality of foods. In order to avoid foodborne illness, the Serbian Regulation on General and Special Conditions for Food Hygiene (Official Gazette of RS, No. 72/10) provides microbiological criteria for staphylococcal enterotoxins in dairy products.

Стилі APA, Harvard, Vancouver, ISO та ін.

10

BENNETT, REGINALD W. "Atypical Toxigenic Staphylococcus and Non-Staphylococcus aureus Species on the Horizon? An Update†." Journal of Food Protection 59, no. 10 (October 1, 1996): 1123–26. http://dx.doi.org/10.4315/0362-028x-59.10.1123.

Повний текст джерела

Анотація:

Staphylococcal food poisoning is a commonly reported illness caused by the ingestion of preformed staphylococcal enterotoxin in foods, With some exceptions, enterotoxin production is associated with coagulase-positive rather than coagulase-negative staphylococci. Of the coagulase-positive staphylococcal species, S. aureus was historically thought to be exclusively implicated in human foodborne illness. More recently, however, other coagulase-positive and some coagulase-negative staphylococcal species have been associated with foodborne intoxication, Coagulase activity has been used to indicate pathogenicity of a foodborne isolate, and thermostable nuclease is being suggested as a more reliable indictor of enterotoxigenicity. Evidence suggests that the metabolic expressions that are the bases of the tests may not be reliable indicators of pathogenicity. A more useful approach to determine the pathogenicity of a Staphylococcus species is to test directly for enterotoxigenicity with one of the new rapid methods. None of the conventional ancillary identification tests has been conclusively associated with enterotoxin synthesis. Furthermore, evidence exists that enterotoxin production is a characteristic of several species in the genus Staphylococcus.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Goetz, Coralie, Yannick D. N. Tremblay, Daphnée Lamarche, Andréanne Blondeau, Annie M. Gaudreau, Josée Labrie, François Malouin, and Mario Jacques. "Coagulase-negative staphylococci species affect biofilm formation of other coagulase-negative and coagulase-positive staphylococci." Journal of Dairy Science 100, no. 8 (August 2017): 6454–64. http://dx.doi.org/10.3168/jds.2017-12629.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Chou, Po-Yen, Duane Robinson, Amy S. Kapatkin, Steven E. Epstein, Michelle A. Giuffrida, and Barbara A. Byrne. "Susceptibility of Incisional Site Staphylococcus spp. to Three Common Perioperative Antimicrobials in Dogs Undergoing Clean Orthopaedic Procedures." VCOT Open 03, no. 02 (July 2020): e158-e163. http://dx.doi.org/10.1055/s-0040-1716847.

Повний текст джерела

Анотація:

Abstract Objectives The aim of this study was to evaluate the susceptibility pattern of the incisional site Staphylococcus spp. to three commonly used perioperative antimicrobials in dogs. Materials and Methods A cohort of client-owned dogs was included in this prospective observational study. After induction of general anaesthesia and hair clipping, culture samples were collected from the incisional site of healthy dogs before orthopaedic surgery. The isolated colonies were identified. The susceptibility pattern of staphylococcal isolates was determined using Clinical and Laboratory Standard Institute standards. The staphylococcal susceptibilities to amoxicillin/clavulanic acid and cefpodoxime were compared with cefazolin using two-sided equality of proportions test. Results A total of 391 bacterial isolates were identified in 115 dogs. Bacillus spp. (n = 200) and Staphylococcus spp. (n = 95) were the most common bacteria isolated. Coagulase- positive and -negative staphylococci were isolated in 44 and 38% of dogs respectively. Cefazolin, amoxicillin/clavulanic acid and cefpodoxime staphylococcal susceptibilities were 68, 77 and 76% respectively. There were no significant differences in Staphylococcus spp. susceptibility between amoxicillin/clavulanic acid or cefpodoxime and cefazolin (p = 0.19, p = 0.25). Thirty-six per cent of coagulase-negative staphylococci and 6% of coagulase-positive staphylococci isolated were methicillin resistant. Conclusion Cefazolin, amoxicillin/clavulanic acid and cefpodoxime had similar susceptibilities for staphylococcal isolates. Routine use of amoxicillin/clavulanic acid or cefpodoxime in replace of cefazolin should be practiced with caution.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Moura, Tiane Martin de, Fabrício Souza Campos, Pedro Alves d'Azevedo, Sueli Teresinha Van Der Sand, Ana Cláudia Franco, Jeverson Frazzon, and Ana Paula Guedes Frazzon. "Prevalence of enterotoxin-encoding genes and antimicrobial resistance in coagulase-negative and coagulase-positive Staphylococcus isolates from black pudding." Revista da Sociedade Brasileira de Medicina Tropical 45, no. 5 (October 2012): 579–85. http://dx.doi.org/10.1590/s0037-86822012000500008.

Повний текст джерела

Анотація:

INTRODUCTION: Staphylococcal species are pathogens that are responsible for outbreaks of foodborne diseases. The aim of this study was to investigate the prevalence of enterotoxin-genes and the antimicrobial resistance profile in staphylococcus coagulase-negative (CoNS) and coagulasepositive (CoPS) isolates from black pudding in southern Brazil. METHODS: Two hundred typical and atypical colonies from Baird-Parker agar were inoculated on mannitol salt agar. Eighty-two mannitol-positive staphylococci were submitted to conventional biochemical tests and antimicrobial susceptibility profiling. The presence of coagulase (coa) and enterotoxin (se) genes was investigated by polymerase chain reaction. RESULTS: The isolates were divided into 2 groups: 75.6% (62/82) were CoNS and 24.4% (20/82) were CoPS. The biochemical tests identified 9 species, of which Staphylococcus saprophyticus (37.8%) and Staphylococcus carnosus (15.9%) were the most prevalent. Antimicrobial susceptibility tests showed resistance phenotypes to antibiotics widely administered in humans, such as gentamicin, tetracycline, chloramphenicol, and erythromycin. The coa gene was detected in 19.5% (16/82) of the strains and 4 polymorphic DNA fragments were observed. Five CoNS isolates carrying the coa gene were submitted for 16S rRNA sequencing and 3 showed similarity with CoNS. Forty strains were positive for at least 1 enterotoxin-encoding gene, the genes most frequently detected were sea (28.6%) and seb (27.5%). CONCLUSIONS: The presence of antimicrobial resistant and enterotoxin-encoding genes in staphylococci isolates from black pudding indicated that this fermented food may represent a potential health risk, since staphylococci present in food could cause foodborne diseases or be a possible route for the transfer of antimicrobial resistance to humans.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Devriese, Luc A., Marc Vancanneyt, Margo Baele, Mario Vaneechoutte, Evelyne De Graef, Cindy Snauwaert, Ilse Cleenwerck, et al. "Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals." International Journal of Systematic and Evolutionary Microbiology 55, no. 4 (July 1, 2005): 1569–73. http://dx.doi.org/10.1099/ijs.0.63413-0.

Повний текст джерела

Анотація:

Four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot (Psittacus erithacus timneh) were found to constitute a distinct taxon. 16S rRNA gene sequence analysis revealed that its closest phylogenetic relatives are Staphylococcus intermedius and Staphylococcus delphini. Growth characteristics, biochemical features and DNA–DNA hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, novel Staphylococcus species for which the name Staphylococcus pseudintermedius sp. nov. is proposed. The novel species is commonly confused with S. intermedius in routine diagnostic veterinary bacteriology. Although the strains described were isolated from lesions and show several characteristics typical of pathogenic staphylococci, such as coagulase, DNase and β-haemolysin production, the pathogenic significance of the novel species remains unclear. The type strain, LMG 22219T (=ON 86T=CCUG 49543T), was isolated from lung tissue of a cat.

Стилі APA, Harvard, Vancouver, ISO та ін.

15

dos Santos, Danielle Cabral, Carla Christine Lange, Pedro Avellar-Costa, Katia Regina Netto dos Santos, Maria Aparecida Vasconcelos Paiva Brito, and Marcia Giambiagi-deMarval. "Staphylococcus chromogenes, a Coagulase-Negative Staphylococcus Species That Can Clot Plasma." Journal of Clinical Microbiology 54, no. 5 (February 24, 2016): 1372–75. http://dx.doi.org/10.1128/jcm.03139-15.

Повний текст джерела

Анотація:

Staphylococcus chromogenesis one of the main coagulase-negative staphylococci isolated from mastitis of dairy cows. We describeS. chromogenesisolates that can clot plasma. Since the main pathogen causing mastitis is coagulase-positiveStaphylococcus aureus, the coagulase-positive phenotype ofS. chromogenesdescribed here can easily lead to misidentification.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

EDWARDS, MORVEN S. "Coagulase-Negative Staphylococcal Bacteremia in Neonates: Confusion Continued." Pediatrics 86, no. 2 (August 1, 1990): 320–22. http://dx.doi.org/10.1542/peds.86.2.320.

Повний текст джерела

Анотація:

Colonization of neonates with coagulase-negative staphylococci occurs predictably during the first weeks of life, serving as a potential source of bacteremia in very low birth weight infants.1,2 Clinical features alone are insufficient to distinguish sepsis caused by neonatal pathogens other than coagulase-negative staphylococci3 or to differentiate sepsis from noninfectious disorders in these tiny patients. Inasmuch as coagulase-negative staphylococci are the most frequent blood culture isolates from patients in most neonatal intensive care units4,5 and are a cause of substantial morbidity,6 the statement of Freeman et al7 that "blood cultures positive for coagulase-negative staphylococci are almost four times as likely to be perceived as clinically significant if obtained from extremely premature infants" is not surprising.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

BEATTY, BRUCE R., RALPH J. FARNSWORTH, ARNOLD J. LUND, RICHARD H. LYON, and GILBERT E. WARD. "Medium to Culture and Differentiate Coagulase-Positive and -Negative Staphylococci from Bovine Milk." Journal of Food Protection 48, no. 12 (December 1, 1985): 1019–21. http://dx.doi.org/10.4315/0362-028x-48.12.1019.

Повний текст джерела

Анотація:

A medium which incorporates CAMP factor produced by Streptococcus agalactiae (group B) into sheep blood agar was used to culture and identify coagulase-positive staphylococci from bovine milk. Of 506 staphylococcal isolates from bovine milk, 92.5% of coagulase-positive organisms produced a wide zone of complete hemolysis, whereas 98.9% of coagulase-negative organisms did not. The agreement of this one-step culture and identification test with the standard tube coagulase test was higher than that of the deoxyribonuclease test medium, Baird-Parker egg yolk medium, tellurite glycine medium and slide coagulase tests.

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Gajewska, Joanna, and Wioleta Chajęcka-Wierzchowska. "Biofilm Formation Ability and Presence of Adhesion Genes among Coagulase-Negative and Coagulase-Positive Staphylococci Isolates from Raw Cow’s Milk." Pathogens 9, no. 8 (August 14, 2020): 654. http://dx.doi.org/10.3390/pathogens9080654.

Повний текст джерела

Анотація:

The capacity for biofilm formation is one of the crucial factors of staphylococcal virulence. The occurrence of biofilm-forming staphylococci in raw milk may result in disturbances in technological processes in dairy factories as well as the contamination of finished food products. Therefore, this study aimed to determine the prevalence and characteristics of staphylococcal biofilm formation in raw milk samples and to explore the genetic background associated with biofilm formation in those isolates. The material subjected to testing included 30 cow’s milk samples acquired from farms in the central part of Poland. A total of 54 staphylococcal strains were isolated from the samples, of which 42 were classified as coagulase-negative (CoNS) staphylococci belonging to the following species: S. haemolyticus, S. simulans, S. warneri, S. chromogenes, S. hominis, S. sciuri, S. capitis, S. xylosus and S. saprophyticus, while 12 were classified as S. aureus. The study examined the isolates’ capacity for biofilm formation and the staphylococcal capacity for slime production and determined the presence of genetic determinants responsible for biofilm formation, i.e., the icaA, icaD, bap and eno and, additionally, among coagulase-negative staphylococci, i.e., the aap, bhp, fbe, embP and atlE. Each tested isolate exhibited the capacity for biofilm formation, of which most of them (79.6%) were capable of forming a strong biofilm, while 5.6% formed a moderate biofilm, and 14.8% a weak biofilm. A capacity for slime production was demonstrated in 51.9% isolates. Most of the tested staphylococcal strains (90.7%) had at least one of the tested genes. Nearly half (47.6%) of the CoNS had the eno gene, while for S. aureus, the eno gene was demonstrated in 58.3% isolates. The frequency of the bap gene occurrence was 23.8% and 25% in CoNS strains and S. aureus, respectively. The fbe gene was demonstrated in only three CoNS isolates. The presence of the icaA was only demonstrated in CoNS strains (24.1%), while the icaD was found in both CoNS strains (21.4%) and S. aureus (100%). Among the CoNS, the presence of the embP (16.7%), aap (28.6%) and atlE (23.8%) was demonstrated as well. The obtained study results indicate that bacteria of the Staphylococcus spp. genus have a strong potential to form a biofilm, which may pose a hazard to consumer health.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Costanzo, Nicola, Carlotta Ceniti, Adriano Santoro, Maria Teresa Clausi, and Francesco Casalinuovo. "Foodborne Pathogen Assessment in Raw Milk Cheeses." International Journal of Food Science 2020 (January 22, 2020): 1–5. http://dx.doi.org/10.1155/2020/3616713.

Повний текст джерела

Анотація:

General hygienic parameters and selected foodborne pathogens in raw milk cheeses at the retail level were evaluated. A total of 245 raw milk cheese samples were analysed for total bacterial count, Enterobacteriaceae, E. coli, Salmonella spp., Listeria monocytogenes, coagulase-positive Staphylococci, and staphylococcal enterotoxin. Results showed only 3 samples that were not compliant with European rules on staphylococcal enterotoxin, but coagulase-positive Staphylococci were evidenced in all samples tested. Salmonella spp. and Listeria monocytogenes were never detected whereas E. coli was evidenced in 20 samples. Results suggest a need for improvement of good manufacturing practice and milking operation.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Savini, Vincenzo, Cecilia Passeri, Giorgia Mancini, Ornella Iuliani, Roberta Marrollo, Angela Valentina Argentieri, Paolo Fazii, Domenico D'Antonio, and Edoardo Carretto. "Coagulase-positive staphylococci: my pet's two faces." Research in Microbiology 164, no. 5 (June 2013): 371–74. http://dx.doi.org/10.1016/j.resmic.2013.02.004.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Mohammed, Suhad Hadi, Mohammed Neama Hmood, and Areej Abbas Abd. "Screening of nasal carriage for Staphylococcus aureus and their resistance to oxacillin and cefoxitin among medical students in Karbala University." Journal of Contemporary Medical Sciences 1, no. 1 (March 26, 2015): 13–16. http://dx.doi.org/10.22317/jcms.v1i1.56.

Повний текст джерела

Анотація:

Objective Carriage of Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA) is a problem within healthcare organizationsand in the community. The aim of the study was to screen S. aureus carriage and their susceptibility to cefoxitin and oxacillin amongmedical students.Methods A total of 100 nasal swabs were collected. Isolation and identification of the isolate as S. aureus was done using Gram stain,coagulase test and catalase test. S. aureus isolates were confirmed as MRSA using cefoxitin (30 Î¼g) disc and oxacillin (30 Î¼g) disc by Kirby-Bauer disc diffusion method on Mueller-Hinton agar. From 100 nasal swabs, 76 were coagulase negative Staphylococci and 20 werecoagulase positive Staphylococci.Results From 100 nasal swabs, 76 were coagulase negative Staphylococci and 20 were coagulase positive Staphylococci. From these, 60%and 40% were oxacillin- and cefoxitin-resistant isolates, respectively. The data obtained from this study revealed that there were carriers ofMRSA among the medical students.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Savic-Rajic, Natasa, and Vera Katic. "Susceptibility of coagulase positive staphylococci isolated from cow's mammary gland to antibacterial drugs." Veterinarski glasnik 63, no. 5-6 (2009): 299–310. http://dx.doi.org/10.2298/vetgl0906299s.

Повний текст джерела

Анотація:

Coagulase positive staphylococci are one of the most common causes of chronic udder infection. Indiscriminate use of antimicrobial drugs and their presence in the environment where animals live has led to coagulase positive staphylococci strains resistant to antimicrobial means. Proper and timely treatment of sub-clinical mastitis, based on the most effective use of antimicrobial drugs, is the key to good health of the milk herd. The aim was to determine the antimicrobial efficacy of selected assets in relation to coagulase positive staphylococci isolated from samples of milk taken from individual udder quarters of cows in cases of udder infection from three farms with different mastitis prevalence. From a total of 9245 samples of milk taken from individual udder quarters of cows from three farms, 852 strains isolated were coagulase positive staphylococci. Coagulase positive staphylococci were isolated on blood agar and identified on the basis of macro-morphological characteristics and the coagulase and catalase test. The sensitivity of the coagulase positive staphylococci was tested by the Kirby Bauer agar diffusion method with the following antimicrobials: penicillin 6?g, amoxicillin / sulbactam (20 +10?g), cloxacillin 25 ?g, cefalexin 30 ?g, ceftiofur 30?g, linkomycin 15?g, 30 ?g gentamycin and tetracycline 30 ?g. Sensitivity testing of coagulase positive staphylococci, isolated in cases of intramammary cow infections, established a high degree of sensitivity in vitro towards penicilinasa resistant drugs (amoxicillin-sublactam, cloxacilin), cephalosporins of the first and third generations and linkomycin. The highest levels of resistance to penicillin (70.4%) were found on a farm with a moderate prevalence of udder infection, then on the farm with the highest prevalence of intramammary infections (60.2%) and the lowest on the farm with controlled levels of resistance of infection (43.7%). .

Стилі APA, Harvard, Vancouver, ISO та ін.

23

NAYLOR, PAUL T., QUENTIN N. MYRVIK, and ANTHONY GRISTINA. "Antibiotic Resistance of Biomaterial-Adherent Coagulase-Negative and Coagulase-Positive Staphylococci." Clinical Orthopaedics and Related Research &NA;, no. 261 (December 1990): 126???133. http://dx.doi.org/10.1097/00003086-199012000-00015.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

HELTBERG, OLE, and BRITA BRUUN. "POLYMYXIN SUSCEPTIBILITY IN STAPHYLOCOCCI DIFFERENTIATING COAGULASE-POSITIVE AND COAGULASE-NEGATIVE STRAINS." Acta Pathologica Microbiologica Scandinavica Series B: Microbiology 91B, no. 1-6 (August 15, 2009): 157–61. http://dx.doi.org/10.1111/j.1699-0463.1983.tb00026.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Kirchhoff, Louis V., and John N. Sheagren. "Epidemiology and Clinical Significance of Blood Cultures Positive for Coagulase-Negative Staphylococcus." Infection Control 6, no. 12 (December 1985): 479–86. http://dx.doi.org/10.1017/s0195941700063591.

Повний текст джерела

Анотація:

AbstractCoagulase-negative staphylococci are frequently isolated from blood cultures. As these organisms may occasionally cause serious disease, differentiating bacteremia from contamination is very important but often difficult. Over a 26-month period, of 29,542 blood cultures processed at the University of Michigan Medical Center, 2,875 (9.7%) were positive, and of those, 694 (from 527 patients) grew coagulase-negative staphylococci. Isolates from the 439 patients with only one blood culture positive for coagulase-negative staphy-lococci and those from the 18 patients with two positive cultures 10 days or more apart were deemed contaminants. Review of the records of the remaining 70 patients with multiple isolates indicated that 33 had had an episode of true bacteremia, 29 (87.9%) of which were associated with intravascular catheters or prosthetic valves. Overall, 85% of all coagulase-negative staphylococci isolated during the study period were judged to be contaminants. Seventy-one percent of the blood cultures drawn during the episodes of bacteremia were positive for coagulase-negative staphylococci as opposed to only 34% in the patients with contaminated cultures (p < 0.01). Moreover, coagulase-negative staphylococci grew in both aerobic and anaerobic bottles in 85% of blood culture sets drawn during episodes of bacteremia, but in only 30% of the cultures thought to be contaminated (p < 0.001). Growth of coagulase-negative staphylococci in less than 48 hours was also significantly associated with bacteremia (p < 0.01). Antibiotic sensitivity patterns were not useful in differentiating bacteremia from contamination. Thus, clinicians should consider coagulase-negative staphylococci as true blood pathogens in patients with intravascular devices who have a high proportion of blood cultures positive for this organism over a short period of time, and whose cultures became positive in less than 48 hours, with a high percent positive in both bottles. Microbiology laboratories can conserve considerable resources by limiting sensitivity studies to isolates from such patients.

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Srinivasan, Arjun, James D. Dick, and Trish M. Perl. "Vancomycin Resistance in Staphylococci." Clinical Microbiology Reviews 15, no. 3 (July 2002): 430–38. http://dx.doi.org/10.1128/cmr.15.3.430-438.2002.

Повний текст джерела

Анотація:

SUMMARY Vancomycin resistance has been reported in clinical isolates of both coagulase-negative staphylococci and Staphylococcus aureus. The emerging threat of widespread vancomycin resistance poses a serious public health concern given the fact that vancomycin has long been the preferred treatment of antibiotic-resistant gram-positive organisms. Though major efforts are now being focused on improving our understanding of vancomycin resistance, there is much that remains unknown at this time. This article reviews the major epidemiologic, microbiologic, and clinical characteristics of vancomycin resistance in both coagulase-negative staphylococci and S. aureus. The review begins with a discussion of issues common to both coagulase-negative staphylococci and S. aureus, such as definitions, laboratory detection of vancomycin resistance, and infection control issues related to vancomycin-resistant staphylococci. The rest of the article is then devoted to a discussion of issues unique to each organism, including epidemiology, risk factors for infection, mechanisms of resistance, and management options.

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Bulajić, Snežana, Zora Mijačević, Tijana Ledina, and Bojan Golić. "Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci." Acta Veterinaria 65, no. 4 (December 1, 2015): 518–37. http://dx.doi.org/10.1515/acve-2015-0044.

Повний текст джерела

Анотація:

AbstractSjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheep’s milk in the southern part of Serbia - Sjenica Pester plateau.The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated.During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 105cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identified asStaphylococcus intermedius(11 isolates) andStaphylococcus aureus(1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profile in coagulase positive staphylococci.Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profile in enterococci.All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins,tet(M) andtet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showedtet(M) gene presence, while 2 lactobacilli isolates revealed the presence oftet(K) gene.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

ELLENDER, RUDOLPH D., LIEPIN HUANG, SANDRA L. SHARP, and ROBERT P. TETTLETON. "Isolation, Enumeration, and Identification of Gram-Positive Cocci from Frozen Crabmeat." Journal of Food Protection 58, no. 8 (August 1, 1995): 853–57. http://dx.doi.org/10.4315/0362-028x-58.8.853.

Повний текст джерела

Анотація:

Bacterial levels in frozen crabmeat samples were determined by plate counts using four staphylococcal isolation media incubated for 24, 48, and 72 h at 26 and 35°C. Staphylococcal counts determined by the spread-plate Food and Drug Administration Baird-Parker protocol incubated at 35°C for 48 h (FDABP48-35) served as the standard for comparison. When FDABP48-35 counts were compared to counts from 29 combinations of media, time of incubation, and incubation temperature, only FDABP and Borrego, Florido, Mrocek, and Romero (BFMR) counts, representing 11 combinations, were statistically comparable to FDABP48-35 counts. Cocci (91.5%) were the dominant bacterial type; gram-positive rods (8.3%) and gram-negative isolates (0.2%) were also detected. Isolates tested by the coagulase reaction were predominantly coagulase negative (CN) (97.7%). Of 100 isolates analyzed by the BIOLOG identification procedure, 62% were classified as Staphylococcus lentus, S. hominis, and S. epidermidis. Three isolates were identified as Staphylococcus aureus. These data indicate that species identification of staphylococci from crabmeat can assist in determining the source of contamination, and that staphylococcal isolates from crabmeat are more likely to be coagulase negative.

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Elnageh, Hiam R., Murad A. Hiblu, Mohamed Salah Abbassi, Yousef M. Abouzeed, and Mohamed O. Ahmed. "Prevalence and antimicrobial resistance of Staphylococcus species isolated from cats and dogs." Open Veterinary Journal 10, no. 4 (February 5, 2021): 452–56. http://dx.doi.org/10.4314/ovj.v10i4.13.

Повний текст джерела

Анотація:

Background: Methicillin-resistant staphylococci (MRS) are an emerging global problem with serious public health concern.Aims: This study investigated the prevalence and antimicrobial susceptibility of commensal Staphylococcus species isolated from healthy and clinical cats and dogs.Methods: Nasal swab samples were collected from animals and processed using selective and semi-selective mediums. Presumptive isolates were subjected to biochemical testing and analyzed using the Phoenix automated identification and susceptibility testing system. PCRs protocols were used to screen for mecA and pvl genes.Results: In total, 151 pets (103 cats and 48 dogs) were enrolled, of which 14 dogs (29%) and 24 cats (23%) were colonized with various Staphylococcus species mainly originated from healthy animals. A total of 38 staphylococci isolates were collected and distributed between 24 coagulase-negative and 14 coagulase-positive staphylococci. Only 13 staphylococci strains were identified as MRS, out of which only five isolates expressed that the mecA gene exclusively originated from healthy pets.Conclusion: This is the first study reporting the prevalence and colonization status of staphylococci species and MRS strains isolated from cats and dogs in Libya. The study reports important information of medical and clinical importance on antimicrobial and multidrug resistance of different staphylococci strains, particularly the coagulase negative species.

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Bhakdi, S. "Positive camp-phenomenon elicited by coagulase-negative staphylococci." Medical Microbiology and Immunology 174, no. 5 (December 1985): 275–79. http://dx.doi.org/10.1007/bf02124812.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Borelli, B. M., I. C. A. Lacerda, L. R. Brandão, C. R. Vianna, M. C. Ferreira, F. C. O. Gomes, L. S. Carmo, L. G. D. Heneine, and C. A. Rosa. "Identification of Staphylococcus spp. isolated during the ripening process of a traditional minas cheese." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 63, no. 2 (April 2011): 481–87. http://dx.doi.org/10.1590/s0102-09352011000200028.

Повний текст джерела

Анотація:

The population dynamics of Staphylococcus spp. was studied during the ripening of Canastra Minas cheese at three farms located in the State of Minas Gerais, Brazil. The presence of coagulase (coa), thermonuclease (nuc), and enterotoxin (sea, seb, sec, and sed) genes was investigated in Staphylococcus strains isolated during the 60-day cheese-ripening period. The presence of the staphylococcal enterotoxins A, C, and D was also investigated in the cheese samples. Cheese samples that were matured for 0, 7, 15, 30, and 45 days presented staphylococci counts from 10³ to 10(8)cfu/g. All isolates considered coagulase-positive by physiological tests had the coa gene. However, no association was observed between the results obtained with biochemical tests and those obtained by PCR using gene-specific primers for coagulase-negative strains. Coagulase and thermonuclease genes occurred simultaneously in 41.3% of Staphylococcus spp. tested. None of the investigated Staphylococcus strains expressed enterotoxins SEA, SEB, SEC, and SED. Enterotoxins A, C, and D were not detected in any of the cheese samples.

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Kaplan, N. M. "Use of thermonuclease testing to identify Staphylococcus aureus by direct examination of blood cultures." Eastern Mediterranean Health Journal 9, no. 1-2 (April 2, 2003): 185–90. http://dx.doi.org/10.26719/2003.9.1-2.185.

Повний текст джерела

Анотація:

Blood cultures submitted to the Clinical Microbiology Laboratory, Queen Alia Military Hospital, Amman during 1999-2001 were examined to evaluate thermonuclease testing for identifying Staphylococcus aureus in blood culture broths growing gram-positive cocci. Of 170 cultures studied, 129 yielded gram-positive staphylococci and 41 yielded other gram-positive cocci. Toluidine blue-deoxynucleic acid agar plates were used to test for thermonuclease activity. St and ard tube coagulase tests were performed on the isolates. Direct detection of thermonuclease activity in 76 blood culture broths containing gram-positive staphylococci showed 100% correlation with subsequent tube coagulase tests. The thermonuclease test provides a fast, specific and reliable confirmation of S. aureus bacteraemia by direct examination of blood culture broths that contain gram-positive cocci. This allows for timely, optimal antibiotic therapy

Стилі APA, Harvard, Vancouver, ISO та ін.

33

UGWU, Ifeoma Chinyere, Madubuike Umunna ANYANWU, Chidozie Clifford UGWU, and Jude Chukwuemeka OKORO. "Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria." Notulae Scientia Biologicae 7, no. 1 (March 20, 2015): 20–25. http://dx.doi.org/10.15835/nsb719479.

Повний текст джерела

Анотація:

This study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples were processed for isolation and identification of methicillin-resistant Staphylococcus species, following standard methods. Confirmation of methicillin-resistance by the isolates was done using penicillin binding protein 2a (PBP2a) kit. Phenotypic resistance of the isolates to antimicrobial agents was determined using disc diffusion method. Out of 202 samples processed, 200 (99.01%) yielded positive growth of staphylococci on oxacillin-supplemented oxacillin-resistance staphylococcal agar base (ORSAB). A total of 200 methicillin-resistant staphylococcal isolates were obtained. Of these, 91 (45.5%) were identified as methicillin-resistant coagulase-positive Staphylococcus (MRCoPS), while 109 (54.5%) were identified as methicillin-resistant coagulase-negative Staphylococcus species (MRCoNS). Out of the 91 MRCoPS, 53 (58.2%) were identified as methicillin-resistant Staphylococcus aureus (MRSA). Resistance of the isolates was 99.5% to erythromycin and chloramphenicol, 100% to oxacillin, 76.5% to gentamicin, 96.5% to clindamycin, 92.5% to ciprofloxacin, 99% to sulphamethoxazole/trimethoprim and tetracycline, and 98.5% to streptomycin and cefoxitin. All the isolates were multidrug resistant. This study has shown that healthy broilers reared and slaughtered in Nsukka Southeast, Nigeria harbour multidrug-resistant MRS and thus serve as their reservoirs.

Стилі APA, Harvard, Vancouver, ISO та ін.

34

BUCUR, Iulia Maria, Viorel HERMAN, Corina PASCU, Ionita IANCU, Janos DEGI, and Nicolae CATANA. "Research on the Phenotypic Characterization of Mrsa Strains Isolated from Animals." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 74, no. 1 (May 18, 2017): 88. http://dx.doi.org/10.15835/buasvmcn-vm:12610.

Повний текст джерела

Анотація:

Keywords: chromogen, methicillin, MRSA, resistanceIntroduction: Currently, both in staphylococci isolated from animals with different diseases, as well as in humans, the MRSA strains (Methicillin Resistant S. aureus) are monitored, as the methicillin resistance is associated with the resistance to other antibiotic groups.Methicillin resistance is encoded by mec staphylococcal chromosomal cassettes (SCCmec), which are islands of resistance. These strains can be identified by molecular biology tests and tests that reveal several phenotypic characteristics.The research was made in order to characterize and identify phenotypically the MRSA staphylococci strains isolated from animals.Materials and Methods: Researches were made on 240 coagulase positive and coagulase negative strains of staphylococci. Mannitol fermentation was tested on Champan medium, free coagulase was revealed on Baird-Parker medium and to identify S. aureus subsp. aureus was used the chromogenic medium Chromatic Staph.Methicillin-resistant strains were detected by disc diffusion method, using biodiscs with methicillin, oxacillin and cefoxitin. Also, to identify the MRSA strains, was used the chromogenic medium Chromatic MRSA.Results: The isolates were positive to mannitol and produced complete haemolysis or were unhaemolytic. A total of 44 strains produced free coagulase on Baird-Parker medium, considered coagulase positive strains, while 196 were coagulase negative strains.The isolates conducted differently to methicillin: 22,08% of strains were resistant, 51,25% of strains were susceptible and 26,66% had intermediate resistance, while the resistant strains to oxacillin were 42,91%.The increased frequency of methicillin-resistant strains of staphylococci and, particularly, MRSA strains, determined using the cefoxitin disk diffusion test, which is more reliable than methicillin and oxacillin.On the MRSA chromogenic medium, the methicillin-resistant strains of staphylococci formed colonies with pigment from mauve to orange mauve.Conclusion: The obtained results by disk diffusion test on resistance patterns to 3 beta-lactams, resistant to penicillinase, indicated a different frequency of the resistant strains to these antibiotics.Cefoxitin disk diffusion test revealed a frequency of 2,51% of resistant strains, that can be considered MRSA strains.

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Suepaul, Sharianne, Karla Georges, Chandrashekhar Unakal, Filip Boyen, Jamie Sookhoo, Khalil Ashraph, Anisah Yusuf, and Patrick Butaye. "Determination of the frequency, species distribution and antimicrobial resistance of staphylococci isolated from dogs and their owners in Trinidad." PLOS ONE 16, no. 7 (July 2, 2021): e0254048. http://dx.doi.org/10.1371/journal.pone.0254048.

Повний текст джерела

Анотація:

The close contact between humans and their dogs can lead to the commingling of staphylococci and the exchange of mobile genetic elements encoding antimicrobial resistance. The objectives of this study were to determine the species distribution and antimicrobial resistance patterns of staphylococci colonizing canine pets and their owners in Trinidad. Staphylococci were isolated from canine pets and their owners and identified using MALDI-TOF mass spectrometry. Antimicrobial susceptibilities were determined using the Kirby-Bauer disc diffusion method against seven classes of antimicrobial agents. A total of 440 staphylococci were isolated from 112 canine pets and their owners, 53.4% were from canine pets and 46.6% were from owners. Twenty-four species were detected, of which, most isolates (32.5%) belonged to the Staphylococcus intermedius group (SIG). S. sciuri was the most common species of coagulase-negative staphylococci (CoNS) comprising 22.3% of all isolates. Antimicrobial resistance was highest against commonly used antimicrobials, such as penicillin (51.4%), tetracycline (26.1%) and trimethoprim/sulfamethoxazole (18.6%). These antimicrobials also comprised the most common multidrug resistance (MDR) combination. Overall, 19.1% of isolates displayed multidrug resistance. No methicillin-resistant Staphylococcus aureus (MRSA) isolates were detected. However, methicillin resistance was detected in 13.3% and 15.1% of coagulase-positive staphylococci (CoPS) and the CoNS+CoVS (combined CoNS and coagulase-variable staphylococci) group respectively. The presence of methicillin-resistant staphylococci is worrisome because there is the potential for the transfer of these strains between dogs and humans. These strains may act as a reservoir of resistance genes.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Beekmann, Susan E., Daniel J. Diekema, and Gary V. Doern. "Determining the Clinical Significance of Coagulase-Negative Staphylococci Isolated From Blood Cultures." Infection Control & Hospital Epidemiology 26, no. 6 (June 2005): 559–66. http://dx.doi.org/10.1086/502584.

Повний текст джерела

Анотація:

AbstractBackground and Objective:Coagulase-negative staphylococci are both an important cause of nosocomial bloodstream infections and the most common contaminants of blood cultures. Judging the clinical significance of coagulase-negative staphylococci is vital but often difficult and can have a profound impact on an institution's bloodstream infection rates. Our objective was to develop an algorithm to assist in determining the clinical significance of coagulase-negative staphylococci.Design:A single experienced reviewer examined the medical records of 960 consecutive patients with positive blood cultures in a tertiary-care referral teaching hospital. Four hundred five of the cultures contained coagulase-negative staphylococci. A determination of clinical significance was made and the performances of various published algorithms that contained readily available clinical and laboratory data were compared.Results:Eighty-nine (22%) of the episodes were considered significant, whereas 316 were contaminants. Patients with bacteremia were significantly more likely to be neutropenic and exhibit signs of sepsis syndrome. The algorithm with the best combined sensitivity (62%) and specificity (91%) for determining the clinical significance of coagulase-negative staphylococci was defined as at least two blood cultures positive for coagulase-negative staphylococci within 5 days, or one positive blood culture plus clinical evidence of infection, which includes abnormal white blood cell count and temperature or blood pressure.Conclusion:Use of this algorithm could potentially reduce misclassification of nosocomial bloodstream infections and inappropriate use of vancomycin for positive blood cultures likely to represent contamination (Infect Control Hosp Epidemiol2005;26:559-566).

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Leung, Michael J., Nichalas Nuttall, Margaret Mazur, Tania L. Taddei, Michael McComish, and John W. Pearman. "Case of Staphylococcus schleiferi Endocarditis and a Simple Scheme To Identify Clumping Factor-Positive Staphylococci." Journal of Clinical Microbiology 37, no. 10 (1999): 3353–56. http://dx.doi.org/10.1128/jcm.37.10.3353-3356.1999.

Повний текст джерела

Анотація:

Staphylococcus schleiferi is a coagulase-negative staphylococcus infrequently reported as a human pathogen. We report a case of prosthetic valve endocarditis attributed to this organism, contrast it to another Staphylococcus species that gives similar clumping factor results (S. lugdunensis), and propose a simple, effective identification scheme for identification of clumping factor-positive staphylococci.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Tanaka, Fernanda Yuri Rodrigues, Fernanda Montanholi de Lira, Samanta Stinghen de Abreu, Stael Málaga Carrilho, Edson Antonio Rios, Ronaldo Tamanini, Elaine Maria Seles Dorneles, Natalia Gonzaga, Rafael Fagnani, and Ulisses de Pádua Pereira. "Characterization of coagulase-positive Staphylococcus spp., antimicrobial resistance profile, and presence of enterotoxin-producing genes in goat milk in Paraná State." Semina: Ciências Agrárias 43, no. 5 (November 17, 2022): 2309–22. http://dx.doi.org/10.5433/1679-0359.2022v43n5p2309.

Повний текст джерела

Анотація:

Goat milk presents interesting characteristics to consumers, but the production of this food faces several challenges that influence its quality. Process failures from milking to processing and commercialization can expose milk to contamination by pathogenic microorganisms, including the coagulase-positive Staphylococcus group. Although Staphylococcus aureus is the most relevant species in mastitis and food poisoning, other species in this group are also important, especially those related to food poisoning. This study aimed to identify the coagulase-positive Staphylococcus species in goat milk using biochemical tests, determine the prevalence of antimicrobial resistance using the disc diffusion test, and investigate enterotoxin-producing genes, sea, seb, sec, sed, see, seg, seh, and sei by multiplex PCR. A total of 384 coagulase-positive Staphylococcus strains obtained from raw goat milk collected from nine farms in Paraná during four seasons of the year were studied. Biochemical tests showed that 85.69% of the 384 strains were S. aureus, followed by 9.38% of S. intermedius, 4.17% of S. hyicus and 0.78% of S. delphini. For the antimicrobial resistance test, up to three strains of each species identified as coagulase-positive Staphylococci were chosen from each farm, with a total of 74 strains. Of these, 27% (20/74) showed resistance to at least one antibiotic, and among all penicillin-resistant strains, 45% (9/20) also presented resistance to tetracycline. In the search for staphylococcal enterotoxin-producing genes, 49 S. aureus strains were studied; among them, 40.81% (20/49) presented enterotoxin-producing genes. The highest prevalence was detected for the sec gene, which was present in 22.44% (11/49) of the strains, followed by the seh gene in 18.36% (9/49), and the see gene was detected in 4.08% (2/49). It was concluded that S. aureus was the predominant species in raw goat milk, with a high prevalence of penicillin- and tetracycline-resistant Staphylococci and a significant number of strains with staphylococcal enterotoxin-producing genes. The strains studied carried enterotoxin-producing genes involved in food poisoning in humans, indicating that goat milk is a possible source of contamination and, therefore, a potential danger to public health.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

de Freitas Guimarães, Felipe, Diego Borin Nóbrega, Virginia Bodelão Richini-Pereira, Pâmela Merlo Marson, José Carlos de Figueiredo Pantoja, and Helio Langoni. "Enterotoxin genes in coagulase-negative and coagulase-positive staphylococci isolated from bovine milk." Journal of Dairy Science 96, no. 5 (May 2013): 2866–72. http://dx.doi.org/10.3168/jds.2012-5864.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Journal, Baghdad Science. "Effect of Subinhibitory concentration of Antibiotic on Bacterial Adherence to Orthopedic Prosthetic Device." Baghdad Science Journal 4, no. 1 (March 4, 2007): 28–34. http://dx.doi.org/10.21123/bsj.4.1.28-34.

Повний текст джерела

Анотація:

The effect of subinhibitory concentration of Antibiotics on the Adherence of S.aureus (Coagulase Positive Staphylococci), and S.epidermidis (Coagulase negative Staphylococci) and Pseudomonas aeruginosa, Enterobacter cloacae, Citobacter freundi (Gram negative bacteria) was done and the results revealed that Rifampicin was the best antibiotic inhibiting Staphylococci adherence and Vancomycin has less effect on the adherence of Staphylococci, whereas Tetracyclin was the best antibiotic inhbiting Gram negative bacteria adherence and Amikacin has the lest less effect on inhibiting bacterial adherence.

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Bell, Susan Givens. "Linezolid." Neonatal Network 28, no. 3 (May 2009): 187–92. http://dx.doi.org/10.1891/0730-0832.28.3.187.

Повний текст джерела

Анотація:

THE MOST RECENT DATA FROM THE National Institute of Child Health and Human Development Neonatal Research Network revealed that in late-onset sepsis events in very low birth weight neonates proven by blood culture, 70 percent were caused by Gram-positive organisms. Coagulase-negative staphylococci accounted for 68 percent of these Gram-positive infections, and Staphylococcus aureus, Enterococcus species, and Group B Streptococcus were isolated in the remainder.1Staphylococcus epidermidis continues to be the most common coagulase-negative Staphylococcus species isolated in culture, and S. capitis, S. warneri, S. haemolyticus and S. hominis have also been implicated in neonatal infection.

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Pyz-Łukasik, Renata, and Waldemar Paszkiewicz. "Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland." Journal of Veterinary Research 62, no. 2 (July 7, 2018): 145–49. http://dx.doi.org/10.2478/jvetres-2018-0023.

Повний текст джерела

Анотація:

AbstractIntroductionThe purpose of this study was to determine the microbiological quality of food fish and its safety for consumers.Material and MethodsThe study included 24 fish representing grass carp, bighead carp, Siberian sturgeon, and wels catfish. Specimens were collected in winter. Aerobic bacteria, psychrophilic, Enterobacteriaceae, Staphylococcus spp., and E. coli counts were made, and the presence of Salmonella spp., L. monocytogenes, S. aureus, and other coagulase-positive staphylococci was investigated.ResultsThe microbiological analysis showed a similar level of aerobic, psychrophilic, and Staphylococcus spp. contamination of the four fish species. The Enterobacteriaceae count was higher in the muscles of grass carp and bighead carp than S. sturgeon and wels catfish. No pathogenic bacteria such as Salmonella spp., E. coli, L. monocytogenes, Staphylococcus aureus, or other coagulase positive staphylococci were found in samples of the examined fish species.ConclusionThe fresh fish examined in this study were of good microbiological quality and there was no health risk for consumers.

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Ogbole, Martha Echioda, James A. Yaza, James A. Ameh, Samuel Mailafia, Olatunde H. Olabode, Bridget J. Adah, Godspower R. Okoh, and Stella Maduike. "Coagulase - Positive Staphylococci Isolated from Cattle in Maiduguri, Nigeria." International Journal of Current Microbiology and Applied Sciences 7, no. 05 (May 10, 2018): 1301–6. http://dx.doi.org/10.20546/ijcmas.2018.705.158.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Roberson, J. R., L. K. Fox, D. D. Hancock, and T. E. Besser. "Evaluation of methods for differentiation of coagulase-positive staphylococci." Journal of Clinical Microbiology 30, no. 12 (1992): 3217–19. http://dx.doi.org/10.1128/jcm.30.12.3217-3219.1992.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Capurro, A., C. Concha, L. Nilsson, and K. Östensson. "Identification of Coagulase-Positive Staphylococci Isolated from Bovine Milk." Acta Veterinaria Scandinavica 40, no. 4 (December 1999): 315–21. http://dx.doi.org/10.1186/bf03547011.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Sánchez-Vizcaíno, Fernando, David Singleton, Philip H. Jones, Bethaney Heayns, Maya Wardeh, Alan D. Radford, Vanessa Schmidt, Susan Dawson, Peter J. M. Noble, and Sally Everitt. "Small animal disease surveillance: pruritus, and coagulase-positive staphylococci." Veterinary Record 179, no. 14 (October 6, 2016): 352–55. http://dx.doi.org/10.1136/vr.i5322.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

47

HÁJEK, V., V. HORÁK, and J. BALUSEK. "Phage typing coagulase-positive staphylococci from rooks and gulls." Research in Veterinary Science 44, no. 2 (March 1988): 247–50. http://dx.doi.org/10.1016/s0034-5288(18)30849-x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Stillman, Richard I., Richard P. Wenzel, and Leigh C. Donowitz. "Emergence of Coagulase Negative Staphylococci as Major Nosocomial Bloodstream Pathogens." Infection Control 8, no. 3 (March 1987): 108–12. http://dx.doi.org/10.1017/s0195941700067278.

Повний текст джерела

Анотація:

AbstractOver an eight year period, 1975 to 1982, 1,843 nosocomial bloodstream infections were identified by routine prospective surveillance at the University of Virginia Hospital (106/10,000 admissions). Despite a decline in overall bloodstream infection rates during the study period (P =.085), bloodstream infections due to gram positive organisms increased from 29 (1975-1978) to 43/10,000 (1979-1982), (P<0.001). Notably, rates for coagulase negative staphylococci increased from 5.2 (1975-1978) to 12.4/10,000 (1979-1982), (P<0.001). In 1982, coagulase negative staphylococci accounted for 17% of all bloodstream infections and were the most frequently isolated pathogens. Sixty-four percent of patients with coagulase negative staphylococci were in critical care units versus 41% with other bloodstream infections (P<.05). The recognition of coagulase negative staphylococci as significant bloodstream pathogens markedly alters the clinician's approach to nosocomial septicemia.

Стилі APA, Harvard, Vancouver, ISO та ін.

49

Maisi, Päivi, та Inna Riipinen. "Use of California Mastitis Test, N-acetyl-β-glucosaminidase, and antitrypsin to diagnose caprine subclinical mastitis". Journal of Dairy Research 55, № 3 (серпень 1988): 309–14. http://dx.doi.org/10.1017/s0022029900028569.

Повний текст джерела

Анотація:

SummaryAnalysis of 448 milk samples (11 herds) from caprine udder halves showed that microorganisms were isolated from 21·8% of the samples. California Mastitis Test (GMT) and N-acetyl-β-glucosaminidase (NAGase) were superior to antitrypsin in detecting subclinical infections. Coagulase-negative staphylococci and micrococci were the main species isolated from halves showing no clinical disease. Coagulase-positive staphylococcal infections were associated with a significant increase of all inflammatory parameters. Significantly increased CMT and NAGase occurred when streptococci, other staphylococci or micrococci were present. Infection within one half was reflected as an increase in the inflammatory parameters in the milk of the infected half as well as a slight increase in the inflammation parameters in the adjoining half.

Стилі APA, Harvard, Vancouver, ISO та ін.

50

BARTLETT, PAUL C., GAY Y. MILLER, SUE E. LANCE, and LAWRENCE E. HEIDER. "Use of Bulk Tank and Milk Filter Cultures in Screening for Streptococcus agalactiae and Coagulase-positive Staphylococci." Journal of Food Protection 54, no. 11 (November 1, 1991): 848–51. http://dx.doi.org/10.4315/0362-028x-54.11.848.

Повний текст джерела

Анотація:

The use of a single bulk tank milk culture and a single milk filter culture was studied for their suitability as screening tests for coagulase-positive staphylococci and Streptococcus agalactiae. Bulk tank and bulk tank milk filter cultures were compared to quarter milk cultures taken from individual cows at 49 Ohio dairy herds selected from all Ohio dairy herds by a stratified random sampling scheme. Individual cow quarter milk samples were collected from a sample of all milking cows using a sampling scheme designed to detect an organism present in 2% of quarters, with 95% confidence intervals between 1 and 3%. Seventeen (35%) herds had one or more cows positive for S. agalactiae and 34 (69%) had one or more cows positive for coagulase-positive staphylococci. Using the results of individual cow sampling as the standard, the sensitivity for S. agalactiae was estimated as 23.5% for a single milk filter sample and 35.3% for a single bulk tank milk sample. The sensitivity for coagulase-positive staphylococci was estimated as 52.9% for a single milk filter culture and 41.2% for a single bulk tank milk culture. Based on these results and those of others, it appears that a single bulk tank or milk filter sample has a relatively low sensitivity for both coagulase-positive staphylococci and S. agalactiae, making these poor screening tests for the presence of these pathogens within a dairy herd.

Стилі APA, Harvard, Vancouver, ISO та ін.

Статті в журналах з теми "Coagulase-positive staphylococci"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями