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1
Pabón Pereira, C. P., G. Castañares, and J. B. van Lier. "An OxiTop® protocol for screening plant material for its biochemical methane potential (BMP)." Water Science and Technology 66, no. 7 (October 1, 2012): 1416–23. http://dx.doi.org/10.2166/wst.2012.305.
Повний текст джерелаАнотація:
A protocol was developed for determining the biochemical methane potential (BMP) of plant material using the OxiTop® system. NaOH pellets for CO2 absorption and different pretreatment methods were tested for their influence in the BMP test. The use of NaOH pellets in the headspace of the bottle negatively affected the stability of the test increasing the pH and inhibiting methanization. Sample comminution increased the biodegradability of plant samples. Our results clearly indicate the importance of test conditions during the assessment of anaerobic biodegradability of plant material, considering BMP differences as high as 44% were found. Guidelines and recommendations are given for screening plant material suitable for anaerobic digestion using the OxiTop® system.
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Lallement, Audrey, Aline Siaud, Christine Peyrelasse, Prasad Kaparaju, Blandine Schraauwers, Samuel Maunas, and Florian Monlau. "Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential." Bioengineering 8, no. 11 (November 5, 2021): 176. http://dx.doi.org/10.3390/bioengineering8110176.
Повний текст джерелаАнотація:
Anaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tests. Discrepancies exist among laboratories due to variability of protocols adopted and operational factors used. The aim of this study is to verify the influence of some operational factors (e.g., analysis frequency, trace elements and vitamins solution addition and flushing gas), feedstock conservation and the source of inoculum on BMP. Among the operational parameters tested on cellulose degradation, only the type of gas used for flushing headspace of BMP assays had shown a significant influence on methane yields from cellulose. Methane yields of 344 ± 6 NL CH4 kg−1 VS and 321 ± 10 NL CH4 kg−1 VS obtained from assays flushed with pure N2 and N2/CO2 (60/40 v/v). The origin of inoculum (fed in co-digestion) only significantly affected the methane yields for straw, 253 ± 3 and 333 ± 3 NL CH4 kg−1 VS. Finally, freezing/thawing cycle effect depended of the substrate (tested on biowaste, manure, straw and WWTP sludge) with a possible effect of water content substrate.
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La Rosa, I., R. Fernandez-Martin, D. A. Paz, and D. F. Salamone. "106 EFFECTS OF BONE MORPHOGENETIC PROTEIN 4 (BMP4) AND ITS INHIBITOR NOGGIN ON BOVINE IN VITRO EMBRYO DEVELOPMENT." Reproduction, Fertility and Development 23, no. 1 (2011): 158. http://dx.doi.org/10.1071/rdv23n1ab106.
Повний текст джерелаАнотація:
Bone morphogenetic protein 4 (BMP4) is a member of the BMP family of conserved morphogenes in charge of many events of differentiation (Chen et al. 2004 Growth Factors 22, 233–241) BMP4 is involved in regulation of pluripotency in humans and mice though the role in bovine early embryo development is still undefined. Noggin is a BMP4 inhibitor (Groppe et al. 2002 Nature 420, 636–642) that does not have a specific receptor but functions by directly binding BMP ligands. The objective of this work was to study the effects of BMP4 and Noggin on early bovine embryo development. Cumulus–oocyte complexes (COC) were aspirated from abattoir ovaries and in vitro matured in TCM containing 10% fetal bovine serum (FBS), 2 mM FSH, 20 mM cysteamine, 1% antibiotic- antimycotic (15240, GIBCO, Grand Island, NY, USA) and 0.1 mM sodium pyruvate. Incubation conditions were a 6.5% CO2 humidified atmosphere at 39°C. After 22 h, in vitro fertilization was performed. Briefly, frozen–thawed semen was centrifuged twice at 490 × g and resuspended in B.O. solution to a final concentration of 20 × 106 mL–1 and incubation with COC was performed for 5 h. Presumptive zygotes were randomly cultured in CR2 with 0.3% BSA, free of serum and co-culture (control, n = 217) or supplemented with 100 ng mL–1 of either BMP4 (n = 218) or Noggin (n = 205). Cleavage and blastocyst rates were evaluated at Days 2 and 9 of culture. Blastocysts cell numbers were analysed by nuclear staining with Hoechst 33342. The expression pattern of the transcription factor Oct-4 was studied by immunocytochemistry and confocal microscope analysis in blastocysts. Chi-square tests were applied for cleavage, blastocyst, and hatching rates. One-way ANOVA was used to compare blastocyst cell number and a proportion test was used for Oct-4 expression. For all, P < 0.05 was considered significant. Cleavage rate was significantly lower in the Noggin group compared to control (51.2% v. 62.3%) whereas the BMP group (61.3%) did not differ from control or Noggin groups. Blastocyst rates for the BMP and Noggin groups were statistically lower than control (9.24% and 11.7% v. 20.6%, respectively). Hatching rate for the control group was significantly higher than both BMP and Noggin groups (4.6% v. 1.4% and 0.49%, respectively). Blastocyst cell number did not differ between groups (130, 117, and 128 for control, BMP4, and Noggin groups, respectively). Oct-4 expressing cells over total cell number was lower in BMP (72%; n = 3) and Noggin (72%; n = 3) groups compared to control (83%; n = 3). In our conditions, BMP inhibition with Noggin or addition of exogenous BMP4 negatively affected developmental rates and altered the proportion of pluripotent (Oct-4 positive) cells. Our results demonstrate the importance of a correct balance within the BMP signalling system for proper bovine in vitro embryo development.
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Krenzer, Joseph, Alyson Nelson, Trisha Robakowski, Kevin Grant, Kornelia Galior, and Sarah A. Hackenmueller. "Lipemic Interference in Basic Metabolic Panels: Increasing the Lipemia Index Threshold in Order to Decrease the Frequency of Ultracentrifugation." American Journal of Clinical Pathology 154, Supplement_1 (October 2020): S15. http://dx.doi.org/10.1093/ajcp/aqaa137.027.
Повний текст джерелаАнотація:
Abstract Introduction Lipemia in clinical chemistry samples is a problematic form of interference. Clearing these samples for routine testing can be time consuming and increases the turn-around time for these specimens. In our laboratory, samples with a lipemia index >50 (L-index) are manually inspected and visibly lipemic specimens are cleared by ultracentrifugation. Objective The objective of this study was to determine at what L-index ultracentrifugation of lipemic BMP specimens is necessary prior to sample testing to ensure accurate results. Methods Specimens consisted of routinely ordered basic metabolic panels (BMP) that met current criteria for ultracentrifugation, which included an L-index >50 as measured on the Abbott Architect c8000 and visual lipemia. Specimens meeting these criteria were ultracentrifuged and retested. The difference of the pre-ultracentrifuged and post-ultracentrifuged result was evaluated and put into a percent to find the ‘percent difference’ and evaluated against the total allowable error (TEa) for each analyte. If the difference observed following ultracentrifugation was less than or equal to 50% of the TEa, clearance of lipemia by ultracentrifugation was considered unnecessary. Values from all BMP component tests were analyzed in order to find an L-index threshold at which samples need to be ultracentrifuged which could be applied to the entire panel. The report of lipemic indices for BMPs for the month of January 2020 were extracted from the laboratory information system to evaluate the potential impact of altering the L-index threshold for ultracentrifugation. Results Based on the acceptance criteria of ≤50% of TEa, L-index thresholds for Na, K, Cl, calcium, glucose, creatinine, CO2 and BUN were <203, <410, <287, <387, <410, <285, < 153 and <285, respectively. All the calculated differences or percent differences for each analyte did not exceed 50% of the TEa for a given analyte when the L-index was 150 or less. Adjusting the L-index to 150 and applying it to the 195 lipemic BMP samples in January 2020, would have potentially decreased the number of samples requiring ultracentrifugation to 24 lipemic BMPs (88% reduction). Conclusion These data suggest that an L-index greater than 150 can be used for all analytes within a BMP as the threshold for requiring ultracentrifugation. The BMP is one of the most frequently ordered tests in our laboratory and consistently accounts for a substantial portion of the lipemic samples that require ultracentrifugation. Increasing the L-index at which samples will be ultracentrifuged from 50 to 150 would potentially result in an 88% reduction in one month of BMP samples requiring ultracentrifugation.
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Sloan, Andrew, Issam Hussain, Mohamed El-Sheemy, Mohammad Maqsood, Latif Mubasher, Vikas Sharma, and Oleg Eremin. "In Vitro effect of Bone Morphogenetic Protein-2 (BMP-2) and Cigarette Smoke Extract (CSE) on Osteoblastic Mesenchymal Stem Cells: Beneficial Biological Effects of BMP-2 Negated By CSE." Journal of Bone Biology and Osteoporosis 4, no. 1 (December 20, 2018): 110–20. http://dx.doi.org/10.18314/jbo.v4i1.1387.
Повний текст джерелаАнотація:
Introduction: Clinical and demographic studies have shown that tobacco smoking is a major contributor to non- and delayed-union in fracture healing. The cellular and molecular basis for this is poorly understood, and few studies in human fractures have been undertaken.Aims: To analyse the in vitro biological effects of tobacco smoking at the cellular level within the human fracture microenvironment, with specific regard to mesenchymal stem cell (MSC) proliferation and to ascertain whether the application of bone morphogenetic factor-2 (BMP-2) could be used therapeutically to improve fracture healing.Methods: Fracture haematomas (n=10) were collected from anaesthetised, non-smoking patients who had sustained a tibial fracture, and who were undergoing surgical operative fixation. The semi-solid material was dissected and explanted into tissue culture flasks. Complete culture media was introduced, and cultures were incubated at 37oC in a humidified 5% CO2 environment. Cigarette smoke extract (CSE) was produced and infused into the cell cultures to establish an in vitro smoking environment. A control group (n=10) was set-up and left ntreated by CSE. Harvested, spindle-shaped adherent cells were characterised by immunocytochemistry. Cell populations were counted via flow cytometry to assess and compare proliferation rates between CSE-treated and untreated cell cultures. BMP-2 concentrations (10 and 100 ng/mL (an additional dose of 500 ng/mL in CSE- reated cells)) were infused into cell cultures to enhance in vitro cellular viability, which was analysed by means of the MTT assay.Results: There was a significant reduction in the rate of proliferation of osteoblastic MSCs in CSE-treated cells after 5 days of culture (p < 0.05). At a dose of 100 ng/mL, BMP-2 augmented cellular growth and improved cellular viability in cultures not treated with CSE (p < 0.0001). No significant improvement was seen in CSE-treated cell cultures.Summary: The effect of smoking on bone fracture healing appears to contribute to the inhibition of osteoblast proliferation, which may not be reversible with the therapeutic use of exogenous BMP-2. Moreover, the improvement seen in non-smokers does strengthen the case for smokers to cease using tobacco in the perioperative setting in order that such treatments are rendered more effective.
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Pacheco, Lívia Alencar, Jenniffer Tamayo-Peña, Bruna de Souza Moraes, and Telma Teixeira Franco. "Bioenergy, Electricity, Biogas Production, and Emission Reduction Using the Anaerobic Digestion of Organic Municipal Solid Waste in Campinas, One of the Largest Brazilian Cities." Processes 10, no. 12 (December 10, 2022): 2662. http://dx.doi.org/10.3390/pr10122662.
Повний текст джерелаАнотація:
Anaerobic digestion (AD) is an attractive process for bioenergy production and is considered to be an alternative way to reduce landfills. AD improves municipal solid waste (MSW) management, representing a profitable application of the circular economy and could reduce environmental impact. The methane (CH4) potential of four different organic fractions of MSW—paper (PFW), garden (GFW), food (FFW), and a mixture of these three (OFMSW)—via AD was used to investigate the energy potential and the economic and environmental impact of Campinas. Theoretical and experimental biochemical methane potential (BMP) and substrate biodegradability were determined using the Buswell and Müller equation and the VDI 4630 method. The Gompertz model was used to predict the kinetics of the biochemical processes. The highest experimental BMP (410.7 NmLCH4 gVS−1) and biodegradability (86.6%) were reached with OFMSW. OFMSW can avail an energetic potential of approximately 119 GWh year−1, with a biomethane production equivalent to diesel at 49.9 × 103 m3 year−1, hence, potentially curtailing the CO2 emissions of heavy-duty vehicles by almost 133 kt year−1. The electricity demand for approximately 11% of the households in Campinas could be met by the biogas produced by OFMSW, thus increasing local energy security. The replacement of fossil diesel with biomethane to fuel garbage trucks in Campinas could reduce 25% of the diesel demand.
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Ciller, I. M., U. A. Ciller, and J. R. McFarlane. "177. ANTIBODIES AGAINST BMPR-IB UNCOVERS A PARACRINE FUNCTION FOR THE RECEPTOR IN MALE MOUSE LEYDIG CELL TESTOSTERONE PRODUCTION IN VITRO." Reproduction, Fertility and Development 22, no. 9 (2010): 95. http://dx.doi.org/10.1071/srb10abs177.
Повний текст джерелаАнотація:
The male reproductive system is regulated by pituitary gonadotrophins and local testicular factors including the transforming growth factor-β superfamily members which up-regulate and modulate testosterone production respectively. Type I and type II bone morphogenetic protein (BMP) receptors have been identified in both steroidogenic and non-steroidogenic cells in the testis and have been reported to impact steroid production via their effect on steroidogenic enzymes. In this study we investigated if BMPR-IB had an autocrine or paracine role in testosterone production using BMPR-IB antibodies in testis tissue and Leydig cell culture in vitro. Immature (21d) and mature (60d) mice were sacrificed by CO2 asphyxiation and the testis removed, decapsulated and cultured in basal and equine chorionic gonadotrophin (eCG)-conditioned media for three hours at 32 °C in 5 % CO2, in the presence and absence of anti-BMPR-IB. Additionally, Leydig cells were Percoll purified from adult mouse testicular interstitial cells and cultured for three hours with and without anti-BMPR-IB under human chorionic gonadotrophin (hCG) stimulated or unstimulated conditions. After three hours of incubation the culture media was aspirated into labeled vials and assayed for testosterone using a radioimmunoassay. In adult testicular slice culture, treatment with anti-BMPR-IB resulted in a significant decrease in basal and eCG-stimulated testosterone production by 37 % and 41 % respectively, while having no significant effect on basal or eCG-stimulated testosterone production by the immature testis. In purified Leydig cell culture from adult male mice BMPR-IB immunization had no effect on testosterone production in basal or hCG-stimulated conditions. In conclusion, anti-BMPR-IB significantly reduced testosterone production in adult testicular slice culture but not in cell culture, demonstrating that BMP paracrine signalling from the seminiferous tubules is likely to be important in modulating testosterone production by Leydig cells. Additionally, the paracrine signalling appears to be developmentally regulated only occurring in the adult testis.
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García, E. V., M. Hamdi, A. D. Barrera, M. J. Sánchez-Calabuig, A. Gutiérrez-Adán, and D. Rizos. "83 BONE MORPHOGENETIC PROTEIN SIGNALING DURING INTERACTION OF THE BOVINE EMBRYO WITH OVIDUCTAL EPITHELIAL CELLS IN VITRO." Reproduction, Fertility and Development 29, no. 1 (2017): 149. http://dx.doi.org/10.1071/rdv29n1ab83.
Повний текст джерелаАнотація:
In previous studies, we have demonstrated that different signalling components of bone morphogenetic proteins (BMP) are expressed in an anatomically and temporally regulated fashion in the bovine oviduct. However, a local response of this signalling to the embryo presence has not been elucidated yet. The aim of the present study was to evaluate whether the interaction of the embryo with the oviduct can induce changes in the gene expression of BMP signalling components. For this purpose, we used an in vitro co-culture system of a bovine oviducal epithelial cell (BOEC) monolayer with pre-implantation embryos in 2 developmental time points: before and during the main phase of embryonic genome activation (EGA). Isthmus epithelial cells from post-ovulatory stage oviducts (Day 2–4) were cultured in 500 μL of SOF + 10% FCS in 4-well plates at 38.5°C, 5% CO2, 5% O2, and 90% N2. On Day 6 of culture, medium was replaced with SOF + 5% FCS, and 24 h later BOEC monolayer was cultured in the absence or presence of in vitro-produced embryos from 2- to 8-cell stage [G1 BOEC; 33–54 h post-insemination (hpi)] or from 8- to 16-cell stage (G2 BOEC; 54–98 hpi) in the same conditions. In both groups, a polyester mesh was used to define a local co-culture area, and 30 embryos per well were placed in a 6 × 5 grid over the monolayer. In addition, as control groups, embryos in both developmental stages were cultured either in SOF + 5% FCS (G1 FCS and G2 FCS) or in SOF + 3 mg mL−1 BSA (G1 BSA and G2 BSA). At 54 hpi (G1 BOEC/BSA/FCS) or 98 hpi (G2 BOEC/BSA/FCS), embryos that reached 8- or 16-cell stage, respectively, were transferred to SOF + BSA and cultured until Day 9. The mRNA expression levels of 3 BMP receptors (BMPRIA/IB/II), 2 signalling proteins (SMAD1/5), 1 inhibitor (SMAD6), and 1 target gene (ID2) were analysed by qPCR in 5 samples of BOEC cultured with or without embryos before or during EGA, and in 3 pools of 10 embryos at 8 (54 hpi), 16 (98 hpi), and blastocyst stage (Day 7–8) from all groups. Genes H2A.Z and ACTG1 were used as housekeeping genes, and statistical differences were assessed by ANOVA. The presence of the embryo, irrespective the stage, significantly reduced the expression levels of BMPRIB, BMPRII, SMAD1, SMAD6, and ID2 in BOEC. Embryos that interacted with BOEC before EGA (G1 BOEC) showed a significant increase in the relative abundance of SMAD1 at the 8-cell stage compared with controls. Moreover, embryos that interacted with BOEC during EGA (G2 BOEC) showed a significant increase in the relative abundance of BMPRIB, BMPRII, and ID2 at the 16-cell stage when compared with controls. However, no differences were observed in the mRNA expression levels of BMP signalling components in the blastocysts between groups. In conclusion, local embryo-oviduct interaction in vitro induces changes in the transcriptional levels of BMP signalling, causing a bidirectional response that reduces the expression levels of this signalling in the oviducal cells while increases them in the embryo at early stages. This suggests that BMP signalling pathway could be involved in an early cross-talk between the bovine embryo and the oviduct during first stages of development.
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Hussein, T. S., R. B. Gilchrist, and J. G. Thompson. "327 OOCYTE-SECRETED FACTORS DIRECTLY AFFECT OOCYTE DEVELOPMENTAL COMPETENCE DURING IN VITRO MATURATION OF THE BOVINE CUMULUS - OOCYTE COMPLEX." Reproduction, Fertility and Development 18, no. 2 (2006): 271. http://dx.doi.org/10.1071/rdv18n2ab327.
Повний текст джерелаАнотація:
Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions including proliferation, differentiation, and apoptosis. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this study was to determine if OSFs have a direct influence on bovine oocyte developmental competence during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were obtained by aspiration of >3-mm follicles from abattoir-derived ovaries. IVM was conducted in Bovine VitroMat (Cook Australia, Eight Mile Plains, Brisbane, Australia) supplemented with 0.1 IU/mL rhFSH for 24 h under 6% CO2 in air at 38.5�C. In the first experiment, COCs were co-cultured with denuded oocytes (DOs, 5/COC in 10 �L) beginning at either 0 or 9-h of IVM. To generate the 9-h DO group, COCs were first cultured intact for 9-h and then denuded. In the second experiment, specific OSFs, recombinant bone morphogenetic protein-15 (BMP-15) and growth differentiation factor 9 (GDF-9), were prepared as partially purified supernatants of transfected 293H cells, and used as 10% v/v supplements in Bovine VitroMat. Treatments were: (1) control (no supplement), (2) BMP-15, (3) GDF-9, (4) BMP-15 and GDF-9, and (5) untransfected 293H control. Following maturation, in vitro production of embryos was performed using the Bovine Vitro system (Cook Australia) and blastocysts were examined on Day 8 for development. Developmental data were arcsine-transformed and analyzed by ANOVA, followed by Tukey's test. Cell numbers were analyzed by ANOVA. Co-culturing intact COCs with DOs from 0 or 9 h did not affect cleavage rate, but increased (P < 0.001) the proportion of cleaved embryos that reached the blastocyst stage post-insemination (50.6 � 1.9 and 61.3 � 1.9%, respectively), compared to COCs cultured alone (40.7 � 1.4%). Therefore, paracrine factors secreted by DOs increased the developmental competence of oocytes matured as COCs. OSFs also improved embryo quality, as co-culture of COCs with DOs (0 or 9 h) significantly increased total cell (156.1 � 1.3 and 159.1 � 1.3, respectively) and trophectoderm (105.7 � 1.3 and 109.8 � 0.4, respectively) numbers, compared to control COCs (total = 148 � 1.2, trophectoderm = 98.2 � 0.8, P < 0.001). BMP-15 alone or with GDF-9 also significantly (P < 0.001) increased the proportion of oocytes that reached the blastocyst stage post insemination (57.5 � 2.4% and 55.1 � 4.5%, respectively), compared to control (41.0 � 0.9%) and 293H-treated (27.1 � 3.1%) COCs. GDF-9 also increased blastocyst yield (49.5 � 3.9%) but not significantly. These results are the first to demonstrate that OSFs, and particularly BMP-15 and GDF-9, directly affect bovine oocyte developmental competence. These results have far-reaching implications for improving the efficiency of IVM in domestic species and human infertility treatment, and support the role of OSF production by oocytes as a diagnostic marker for developmental competence.
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Zeng, Shengquan, Riley Harris, and Eunsung Kan. "Effect of Alfalfa-Derived Biochar on Anaerobic Digestion of Dairy Manure." Agronomy 12, no. 4 (April 11, 2022): 911. http://dx.doi.org/10.3390/agronomy12040911.
Повний текст джерелаАнотація:
Biochemical methane potential (BMP) tests were conducted for investigating the effects of alfalfa-derived biochar (AF-BC) on anaerobic digestion (AD) of dairy manure under various loading of AF-BC (0–10 g/L). BMP tests were performed at mesophilic temperature (37 °C) with the addition of AF-BC. Biogas and methane volumes and concentrations, water quality parameters (i.e., COD (chemical oxygen demand)), and volatile fatty acids (VFAs) were measured during the AD process. The addition of 1 and 5 g/L of AF-BC increased the biogas yields by 15.51% and 26.09% and methane yields by 14.61% and 26.88% compared with the control without addition of AF-BC. Additionally, the addition of AF-BC (1–10 g/L) decreased the lag phase by 7.14–22.45% and the CO2 content of biogas by 13.60–32.48%, while increasing the COD removal efficiency by 19.19–35.94% in the AD of dairy manure. Moreover, the addition of AF-BC also decreased total VFAs and acetic acid concentrations in the AD process. The increase in AD performance was mainly owing to the improvement of buffering ability of the AD system and direct interspecies electron transfer (DIET) among AD microorganisms resulting from the addition of AF-BC. In contrast, the addition of 10 g/L AF-BC did not show any obvious improvement in biogas and methane yields in the AD of dairy manure, possibly because of toxic effects from excessive addition of AF-BC toward the AD microorganisms. Therefore, this study supported practical feasibility of AF-BC-enhanced AD of dairy manure.
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Caldas, Lucas Rosse, Maykon Vieira Silva, Vítor Pereira Silva, Michele Tereza Marques Carvalho, and Romildo Dias Toledo Filho. "How Different Tools Contribute to Climate Change Mitigation in a Circular Building Environment?—A Systematic Literature Review." Sustainability 14, no. 7 (March 22, 2022): 3759. http://dx.doi.org/10.3390/su14073759.
Повний текст джерелаАнотація:
The circular economy (CE) has become a trend because concern has arisen regarding the end of life of several products and the reduction of CO2 emissions in many processes. Since the architecture, engineering, and construction (AEC) industry is one of the biggest generators of environmental impacts, there is a need to apply the CE concept to the industry in order to reduce greenhouse gas (GHG) emissions. However, the role of different tools that are used to integrate CE strategies to reduce GHG emissions by the AEC industry is still unknown in the scientific literature. The purpose of this paper is to carry out a systematic literature review on the theme and analyze the following seven tools: (1) life cycle assessment—LCA; (2) building information modeling—BIM; (3) building environmental certifications—BEC; (4) building materials passports—BMP; (5) waste management plan—WMP; (6) augmented reality—AR; and (7) virtual reality—VR. A total of 30 papers were reviewed, and it was observed that, in terms of CE strategies and climate change mitigation, the vast majority can be classified as closing loops and are mainly related to recycling and reuse at the end of life and the use of recycled materials. Considering the building’s stakeholders, constructors, researchers, and designers can be the main users and, consequently, those that most benefit from the use of the evaluated tools. The integration between LCA, BIM, and BMP was also observed. Finally, as one of the main contributions of this research, other types of integration among the analyzed tools are proposed. These proposals seek to improve and update the tools and also address the need to reduce GHG emissions.
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Riungu, Joy, Mariska Ronteltap, and Jules B. van Lier. "Anaerobic stabilisation of urine diverting dehydrating toilet faeces (UDDT-F) in urban poor settlements: biochemical energy recovery." Journal of Water, Sanitation and Hygiene for Development 9, no. 2 (March 20, 2019): 289–99. http://dx.doi.org/10.2166/washdev.2019.099.
Повний текст джерелаАнотація:
Abstract Biochemical energy recovery using digestion and co-digestion of faecal matter collected from urine diverting dehydrating toilet faeces (UDDT-F) and mixed organic market waste (OMW) was studied under laboratory- and pilot-scale conditions. Laboratory-scale biochemical methane potential (BMP) tests showed an increase in methane production with an increase in OMW fraction in the feed substrate. In subsequent pilot-scale experiments, one-stage and two-stage plug flow digester were researched, applying UDDT-F:OMW ratios of 4:1 and 1:0, at about 10 and 12% total solids (TS) slurry concentrations. Comparable methane production was observed in one-stage (Ro-4:1,12%) (314 ± 15 mL CH4/g VS added) and two-stage (Ram-4:1,12%) (325 ± 12 mL CH4/g VS added) digesters, when applying 12% TS slurry concentration. However, biogas production in Ram-4:1,12% digester (571 ± 25 mL CH4/g VS added) was about 12% higher than in Ro-4:1,12%, significantly more than the slight difference in methane production, i.e. 3–4%. The former was attributed to enhanced waste solubilisation and increased CO2 dissolution, resulting from mixing the bicarbonate-rich methanogenic effluent for neutralisation purposes with the low pH (4.9) influent acquired from the pre-acidification stage. Moreover, higher process stability was observed in the first parts of the plug flow two-stage digester, characterised by lower VFA concentrations.
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Czubaszek, Robert, Agnieszka Wysocka-Czubaszek, and Rafał Tyborowski. "Methane Production Potential from Apple Pomace, Cabbage Leaves, Pumpkin Residue and Walnut Husks." Applied Sciences 12, no. 12 (June 16, 2022): 6128. http://dx.doi.org/10.3390/app12126128.
Повний текст джерелаАнотація:
Circular economy aims to eliminate organic waste through its transformation, composting and processing into other products or energy. The main aim of the study was to determine the specific methane yield (SMY) of anaerobic digestion (AD) of four different fruit and vegetable residues (FVR). In addition, the reduction in greenhouse gas (GHG) emissions was calculated based on the assumption that maize will be replaced by the FVR as a feedstock for biogas production. The SMY of four residues (apple pomace, cabbage leaves, pumpkin peels and fibrous strands and walnut husks) was measured in the biomethane potential test (BMP) in wet anaerobic digestion technology. The highest SMY (297.81 ± 0.65 NL kgVS−1) was observed for cabbage leaves while the lowest SMY (131.07 ± 1.30 kgVS−1) was found for walnut husks. The concentrations of two inhibitory gasses (NH3 and H2S) in biogas were low and did not affect the AD process. Only biogas produced from cabbage leaves was characterised by higher NH3 and H2S concentrations resulting from the highest protein concentration in this waste. FVR used as feedstock in biogas production may decrease the area of maize cultivation. Therefore, the GHG emissions from maize cultivation will be reduced. In Poland only, the use of four studied FVR as feedstock for biogas production would contribute to the reduction of GHG emissions by 43,682 t CO2 eq.
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Herzog, K. K., D. J. Milner, S. J. Johnson, and M. B. Wheeler. "329 CHONDROGENIC POTENTIAL OF PORCINE ADIPOSE-DERIVED STEM CELLS, CHONDROCYTES, PERIOSTEAL CELLS, AND FIBROBLASTS IN A PELLET CULTURE SYSTEM." Reproduction, Fertility and Development 27, no. 1 (2015): 253. http://dx.doi.org/10.1071/rdv27n1ab329.
Повний текст джерелаАнотація:
Regenerative medicine has long sought to develop therapies for articular cartilage repair and for enhancing endochondral ossification to address complications of long bone healing. The objective of this study was to determine the chondrogenic potential of porcine primary cell cultures for possible utility in orthopedic tissue engineering applications. Adipose-derived mesenchymal stem cell (ASC), chondrocyte (positive control), periosteal cell, and fibroblast (negative control) primary cell cultures from 8- to 12-month-old Yorkshire pigs were plated at 5000 to 10 000 cells cm–2 in 75-cm2 cell culture flasks using high-glucose Dulbecco's Modified Eagle's Medium (DMEM) supplemented with NaHCO3, 10% fetal bovine serum (FBS), and antimicrobials (penicillin-streptomycin, gentamicin sulfate, and amphotericin B), then incubated at 37°C, 5% CO2, and 18% O2. Cells were trypsinized at ~80% confluency and transferred into 15-mL conical tubes at 500 000 cells tube–1. Suspensions were washed twice by centrifugation in DPBS then condensed via a final centrifugation in 1.0 mL of negative control media (DMEM + 10% FBS) or chondrogenic base media consisting of high-glucose DMEM with 40 µg mL–1 of proline, 50 µM ascorbic acid-2-phosphate, 100 nM dexamethasone, antimicrobials, and 1× insulin-transferrin-selenium solution added. Chondrogenic additives tested were 2% FBS, Kartogenin (200 nM, 400 nM, or 4 µM), 10 ng mL–1 of BMP-4, or a combination of 10 ng mL–1 of BMP-6 + 10 ng mL–1 of TGFβ-3. Pellets were incubated with media changed every 2 to 3 days for a period of 2 to 4 weeks, fixed with 4% paraformaldehyde in DPBS, and then frozen at –80°C in Neg 50 Frozen Section Medium. Eight-micrometer sections were cut using a cryostat onto charged slides. Histochemical staining was performed using hematoxylin and eosin (H&E) for cell morphology and Safranin O and Alcian Blue for cartilage matrix markers. Immunofluorescent staining was done to detect collagen II and aggrecan with the nuclear marker lamin-C used to assess cell viability. Chondrocyte pellets grown in chondrogenic media, regardless of additive, exhibited cartilage matrix formation with H&E and stained strongly positive with Safranin O, Alcian Blue, and collagen II. The ASC pellets grown in chondrogenic media showed mixed cell morphology and areas of early cartilage matrix formation with H&E and stained faintly positive with Safranin O, Alcian Blue, and collagen II at 3 weeks in culture. Periosteal cell pellets had similar morphology in all conditions and did not stain positive for cartilage matrix markers. Fibroblast pellets did not survive any condition for processing. In conclusion, porcine chondrocytes and ASC were able to form cartilage matrix in a pellet culture system with chondrogenic media regardless of additive, while porcine fibroblasts and periosteal cells showed limited to no chondrogenic potential in the conditions tested.
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Hamdi, M., B. Rodríguez-Alonso, A. Almansa-Ordonez, A. Gutierrez-Adán, P. Lonergan, and D. Rizos. "116 In Vitro Transcriptomic Response of Bovine Oviduct Epithelial Cells to Direct or Indirect Embryo Contact." Reproduction, Fertility and Development 30, no. 1 (2018): 197. http://dx.doi.org/10.1071/rdv30n1ab116.
Повний текст джерелаАнотація:
We observed that in vitro transcriptomic response of bovine oviduct epithelial cells (BOEC) to the early embryo could be the result of a contact-dependent signalling effect or interactions with embryo secretions. In order to determine this, BOEC were co-cultured directly with embryos or indirectly with embryo-conditioned media (CM); BOEC from the isthmus of oviducts at early luteal phase were cultured with TCM-199+10% fetal calf serum (FCS) in 4-well plates in 5% CO2 in air at 38.5°C for 6 days until confluence. In vitro 2- and 8-cell embryos as well as their CM were produced in parallel. A day before co-culture, BOEC medium was replaced with SOF+10% FCS. Groups for 2- and 8-cell embryos were established: BOEC in direct contact with embryos; BOEC in the same well as embryos but not in indirect contact; BOEC with embryo CM; and BOEC without embryos, as a control. Polyester mesh was used to maintain embryos position on top of the cells. After 48 h of co-culture, BOEC were recovered for gene expression analysis (4 replicates). The relative abundance of candidate genes previously shown to be affected by the presence of embryo in vivo (Maillo et al. 2015 Biol Reprod. 92, 144) [SMAD6 (BMP signalling pathway); ROCK1, ROCK2 (cytokinesis); SOCS3 (inflammatory response); PRELP (extracellular matrix)] or in vitro (Schmaltz-Panneau et al. 2014 Anim. Reprod. Sci. 149, 103-106) [GPX4, NFE2L2 (oxidative stress); SCN9A (sodium ion binding); EPSTI1 (tissue remodelling); IGFBP3 (insulin-like growth factor binding); TDGF1 (BMP signalling pathway); AGR3 (regulation of ciliary beating)] was assessed by RT-qPCR. H2A.Z and ACTG1 were used as housekeeping genes. Statistical analysis was assessed by ANOVA. The BOEC responded to the presence of 2-cell embryos only when in direct contact by significantly decreasing abundance of NFE2L2. Both direct and indirect embryo contact or culture with CM significantly decreased GPX4, ROCK2, and SCN9A transcripts compared with control. The presence of 2-cell embryos irrespective of being in direct or indirect contact reduced the expression of SMAD6 compared with the control and CM groups. In the case of CM, expression of IGFBP3 was enhanced compared with the control but was similar to the presence of the 2-cell embryos. In the presence of 8-cell embryos, direct contact with BOEC significantly down-regulated the expression for GPX4 and SOCS3, whereas expression of SCN9A was up-regulated. The opposite was observed when compared with control. The presence of 8-cell embryos down-regulated the expression of SMAD6 and ROCK2 compared with the CM group, whereas direct or indirect contact with BOEC or culture with CM down-regulated the expression of PRELP compared to control. In conclusion, these results provide evidence for a differential affect on the transcriptome of BOEC in vitro depending on embryo stage. These changes may be related either with direct embryo contact or embryo secretions released into the media. Research supported by Spanish MINECO-AGL2015-70140-R; AGL2015-66145-R; OECD-Co-operative Programme TAD/CRP JA00092482.
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Malik, H., V. Sharma, S. Saini, S. Guha, and D. Malakar. "225 AUTOLOGOUS TRANSPLANTATION OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE IN ANIMAL." Reproduction, Fertility and Development 28, no. 2 (2016): 244. http://dx.doi.org/10.1071/rdv28n2ab225.
Повний текст джерелаАнотація:
The present study was carried out for isolation and culture of adipose tissue-derived mesenchymal stem cells of goat (gADSC) and dogs (1 dog was suffering from hip dysplasia and another dog from paraplegia) and their characterisation with different markers. Adipose tissue of goat and dog were aseptically isolated and treated with collagenase for 2 h in a CO2 incubator. The enzymatic digested cells were filtered through a 41-µm filter and cells were resuspended in cell culture flask containing medium DMEM/F12, 10% fetal bovine serum, and 50 μg mL–1 gentamycin. In vitro-cultured ADSC were characterised by amplification of mesenchymal stem cell (MSC)-specific surface marker genes of CD44, CD29, and CD166 in PCR and by immunocytochemistry of MSC-specific marker of CD44. For in vitro chondrogenesis, ADSC at passage 3 were incubated in DMEM/F12 containing 100 nM dexamethasone, 1.25 μg mL–1 BSA, and 10 ng mL–1 BMP-4 ITS (insulin-transferrin-selenium) for 3 wk. Chondrogenic differentiation cells were confirmed by Safranin O staining and positive expression of chondrocyte-specific marker genes Aggrecan: primers F-TTGGACTTTGGCAGAATACC and R-CTTCCACCAATGTCGTATCC, and Collagen II: primers F-AACCCTGGAACTGACGGAAT and R-CTCACCCGTTTGACCTTTCG in PCR. Dog ADSC-derived chondrocytes were aseptically injected at 1 × 106 cells kg–1 of BW into dogs with hip dysplasia and paraplegia. Both dogs recovered well after 1 month of autologous transplantation and were able to move freely. Then, 10 dogs having massive wounds were injected with heterologous undifferentiated mesenchymal stem cells at 1 × 106 cells kg–1 of BW and all dogs were cured in an average of 20 days. Then, the paralyzed and fractured dogs were further treated with undifferentiated MSC at 1 × 106 cells kg–1 of BW and most of the dogs were cured properly. These findings may have implications for defining the physiological roles of ADSC in arthritis, some orthopaedic problems, joint regeneration, and neurological disorders and several new applications leading to novel therapeutic opportunities.
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Jiang, Yuehua, April Breyer, Laura Lien, Mark Blackstad, and Catherine Verfaillie. "Culture of Multipotent Adult Progenitor Cells (MAPCs)." Blood 104, no. 11 (November 16, 2004): 2329. http://dx.doi.org/10.1182/blood.v104.11.2329.2329.
Повний текст джерелаАнотація:
Abstract We have previously described the isolation of MAPCs, from human, mouse and rat bone marrow. MAPCs can differentiate into most mesodermal cell types as well as cells with neuroectodermal and endodermal features. However, culture of MAPCs remains laborious and difficult. To address the difficulties in MAPC culture initiation and maintenance, we performed multi-parameter analysis of different steps along the way. First, we evaluated whether collection of bone marrow influences isolation. We found that cells that initiate MAPC cultures appear to be present in higher frequency near growth plates, and near the endosteum. Cells from the middle part of the bone shaft generally can only be cultured short-term and rarely form MAPC lines. Hence, including the ends of the bones and very vigorous flushing of the marrow from the cavity are important. A second parameter we evaluated is cell density both during the initial phases of isolation, i.e. before depleting cells by MACS column, and afterwards. Initial plating before column depletion should be done at relatively higher density, i.e. between 2 and 3x104 cells/cm2. However, after MACS depletion, cell density needs to be kept between 2-5x102 cells/cm2. As MAPC are inclined to form clusters, and it is imperative that cell-cell contact is kept at minimum, it is not sufficient to assure global cell density, but assure that cells are distributed evenly throughout culture vessels. However, maintenance at too low cell densities significantly inhibits proliferation and eventually cells die. This suggests first that paracrine factors produced by the cells are important for their self-renewal, but that cell-cell contact factors, either alone or by secondary release of cytokines, induce cell differentiation. Initial evaluation of gene array studies comparing MAPC maintained at ideal density vs. allowed to grow at high density indicates differential expression of the main three developmental signal pathways, TGFb/BMP, Notch and wnt signaling. These are now being evaluated to determine whether relaxation of the density requirement can be done without loss of potency and self-renewal ability. We also tested the effect of trypsinization. MAPC are very lightly attached cells, whereas differentiating cells are larger and more adherent. Hence short term trypsinization (less than 30sec) and a few gentle taps loosens up the small MAPCs whereas it does not detach the larger cell population. Long-term exposure to trypsin is furthermore toxic to MAPCs; therefore immediate dilution of trypsin with a large volume of MAPC medium is required. On a practical note, as MAPC are small (9–12μm) and are present as a lose layer on top of the pellet, one needs to take care that they are not inadvertently disposed off when centrifuging. We have also found that different fetal calf serum (FCS)-lots significantly affect MAPC growth. In general, FCS that causes faster growth induce differentiation, loss of multi-lineage differentiation potential, and induce at much higher frequency cytogenetic abnormalities, including tetraploidy and aneuploidy. This is more common in mouse than rat MAPC cultures. Hence, as has been shown for hematopoietic progenitors, MSCs and ESCs, screening of serum lots is needed to optimize MAPC culture. A final vital factor is the CO2 and O2 concentration in which cells are maintained. MAPC die under alkaline conditions, hence optimally CO2 concentrations are kept between 5.5– 6%C02. Likewise, we have started to use 5% O2, as this decreases the frequency of cytogenetic abnormalities that occur. Whether this also supports better self-renewal is currently being studied.
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Dubey, A., H. N. Malik, D. K. Singhal, S. Saugandhika, S. Boateng, R. Singhal, S. Fatima, et al. "198 ISOLATION, CHARACTERIZATION, AND IN VITRO DIFFERENTIATION OF GOAT ADIPOSE-TISSUE-DERIVED MESENCHYMAL STEM CELLS INTO PANCREATIC ISLETS-LIKE CELLS." Reproduction, Fertility and Development 26, no. 1 (2014): 213. http://dx.doi.org/10.1071/rdv26n1ab198.
Повний текст джерелаАнотація:
The present study was carried out for isolation of goat (Capra hircus) adipose-tissue-derived stem cells (gADSCs) from adipose tissue, their characterization, and in vitro differentiation of gADSCs into pancreatic islets-like cells by giving conditioned medium. Goat ADSCs were isolated from goat adipose tissue by the enzymatic digestion method and were enriched by filtering through a 41-μm filter. Thus, filtered cells resuspended in a cell culture flask containing growth enriching medium and cultured in 5% CO2 in air at 38.5°C. Goat ADSCs were characterised by amplification of mesenchymal stem cell specific markers i.e. CD29, CD34, CD44, CD90, and CD166 as positive markers and CD41 and CD71 as negative markers. Immunocytochemistry of mesenchymal stem cell was also carried out with specific markers CD44 and CD90. Goat ADSCs were further characterised by in vitro differentiating them into osteocytes, chondrocytes, and adipocytes. For in vitro differentiation of gADSCs into osteocytes gADSCs were supplemented with conditioned medium i.e. DMEM containing fetal bovine serum (FBS), dexamethazone, B-glycerol phosphate and L-ascorbic acid. Osteogenic differentiation was confirmed by positive Alizarin red S staining and amplification of Osteopontin and Collagen I genes. For differentiation into chondrocytes cells, gADSCs were incubated in DMEM/F12 containing dexamethazone, ITX, BMP-4, and FBS for 21 days. Differentiated cells were confirmed by positive Safranin O staining and expression of chondrocytes specific Collagen III and Aggrecan genes. For adipogenesis, gADSCs were incubated with DMEM/F12 containing FBS, dexamethasone, and ITX and differentiated cells were confirmed by positive Oil Red O staining and amplification of adipocytes specific genes i.e. LPL, PPRγ and PPRα. For in-vitro differentiation gADSCs into pancreatic islets-like cells on the third or fourth passage gADSCs were incubated in conditioned medium containing serum-free DMEM/F12 medium with glucose (17.5 mM) in the presence of nicotinamide (10 mM), activin-A (2 nM), exendin-4 (10 nM), pentagastrin (10 nM), retinoic acid (10 μM) and mercaptoethanol (20 μM). The in vitro differentiation gADSCs into pancreatic islets-like cells was confirmed by amplification of pancreatic endoderm specific genes i.e. igf-1, sst, ngn3, pdx-1, isl-1, c-kit, thy-1, and Glut-2, and no expression was detected for above endoderm specific genes in undifferentiated gADSCs. Pancreatic islets-like cells were further characterised by immunostaining and Western blotting of Pdx-1, insulin, and Islets-1 specific protein. It could be concluded that gADSCs was differentiated into different lineages and secretory insulin was produced from pancreatic islets-like cells.
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La Rosa, I., R. Fernandez y Martín, D. A. Paz, and D. F. Salamone. "344 EFFECTS OF BMP4 AND ITS INHIBITOR, NOGGIN, ON OOCYTE MATURATION AND DEVELOPMENT OF BOVINE PREIMPLANTING EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 328. http://dx.doi.org/10.1071/rdv22n1ab344.
Повний текст джерелаАнотація:
BMP4 regulates different events during development in all vertebrates and Noggin is one of its powerful inhibitors that blocks BMP4 interaction with its receptors (Groppe et al. 2002). In this work, the effect of these factors on bovine oocyte maturation and subsequent embryo development has been investigated. COCs were aspirated from abattoir ovaries and in vitro-matured for 22 h or 24 h in a 5% CO2 humidified atmosphere at 39°C in TCM containing 0.6% BSA, 2 mM FSH, 10 mM cysteamine, 1% antibiotic and 1% pyruvate, control group (C), plus 100 ng mL-1 of BMP4 (B), or 100 ngmL-1 of Noggin (NOG). Oocytes were stained with Hoechst 33342 and classified by their nuclear stage. Effects on embryo development were investigated for embryos produced by parthenogenic activation (PA) and IVF For PA, denuded oocytes were chemically activated in 5 μM ionomycine for 4 min, and immediately incubated in 1.9 mM of 6-dimethilaminopurine for 3 h. For IVF, frozen-thawed semen was centrifuged and resuspended in Bracket and Oliphant (BO) solution and incubated with 22 h matured COCs for 5 h. Embryos were cultured in CR2 medium free of serum and co-culture. Cleavage and blastocyst formation were registered at Day 2 and 9 respectively. Fischer’s exact test was used and P ≤ 0.05 was considered significant. Nuclear progression was not affected by maturation treatments [% of MII: 79.4(C, n = 102), 72.4 (B, n = 98), 80.9 (NOG, n = 89)]. For PA, both factors significantly increased cleavage rates [%: 51.7 (C, n = 284), 65 (B, n = 186), 62.1 (NOG, n = 198)] while blastocyst rates were not affected [%: 8.8 (C), 7.5 (B), and 8.6 (NOG)]. On the other hand, for IVF, cleavage rate was statistically lower for Noggin group [%: 70.7 (C, n = 140), 71.3 (B, n = 157), 64 (NOG, n = 159)] while blastocyst rates were similar between groups [%: 15.7 (C), 13.4 (B), 14.5 (NOG)]. Any of the added factors affected cell number of the embryos at Day 2. Blastocysts did not differ in the number of cells at Day 9 (Student’s t-test was used) neither for PA [mean ± SD: 100 ± 33 (C, n = 9), 88 ± 14 (B, n = 3) and 68 ± 8,(NOG, n = 3)] nor for IVF [mean ± SD: 90 ± 24 (C, n = 9), 132 ± 18 (B, n =4) and 99 ± 8 (NOG, n = 3)]. It is noticeable that addition of these factors during in vitro maturation showed different effects on subsequent embryo development depending on whether the embryos were PA or IVF. Probably, these responses represent differences in the BMP signaling system between these embryos which could be associated with different imprinting pattern. Further experiments are needed to elucidate clearly the mechanisms implicated. To our knowledge, this is the first work to study BMP4 inhibition during bovine in vitro maturation. To “Merlo” and “Nueva Escocia” Slaughterhouses
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Jirásek, Jakub, Dalibor Matýsek, and Aneta Minaříková. "Fosfohedyfán z opuštěného železnorudného ložiska Hraničná (Slezsko, Česká republika)." Bulletin Mineralogie Petrologie 28, no. 1 (2020): 44–47. http://dx.doi.org/10.46861/bmp.28.044.
Повний текст джерелаАнотація:
Abandoned iron skarn deposit Hraničná is located 16 km NW of Jeseník, Silesia, Czech Republic. It is situated in the Staré Město Crystalline Complex, belt of high grade metamorphic rocks, which suppose to be a meta-ophiolite of the initial Cambro-Ordovican rifting. The deposit itself is formed by two stratiform magnetite-hematite bands within the marbles and quartz-rich biotite gneisses. Marbles containing silicates are rich in Zn and Pb and give evidence for sedimentary of volcanosedimentary origin of the ore accumulation. We collected several samples at the adit and +20 m levels of the mine which yielded phosphohedyphane. Mineral forms irregular aggregates up to 100 μm within the calcite-dolomite-magnetite skarn. Its average chemical formula from 7 WDS spots is (Ca2.07Sr0.03Ba0.01Mg0.02Pb3.23Zn0.01Fe0.09Al0.01)Σ5.47[(PO4)2.53(AsO4)0.03(SO3)0.01(SiO4)0.24]Σ2.81[Cl1.05F0.20]Σ1.25 based on 13 O+Cl+F. Use of the normalization to Ca1 + Ca2 = 5 and employing the charge balance could lead to the possible presence of (CO3)2- up to 0.60 apfu, resp. 3.61 hm. % CO2; this possible content do not have any effect on mineral classification. It is, therefore, fifth reported occurrence of this mineral in the territory of the Czech Republic and the Bohemian Massif.
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Lithourgidis, Antonios A., Vasileios K. Firfiris, Sotirios D. Kalamaras, Christos A. Tzenos, Christos N. Brozos, and Thomas A. Kotsopoulos. "Energy Conservation in a Livestock Building Combined with a Renewable Energy Heating System towards CO2 Emission Reduction: The Case Study of a Sheep Barn in North Greece." Energies 16, no. 3 (January 18, 2023): 1087. http://dx.doi.org/10.3390/en16031087.
Повний текст джерелаАнотація:
Cold stress in sheep is usually overlooked, even though the animals’ welfare and productivity are affected by low temperatures. The aim of this research was to find out if and to what extent the temperature inside a sheep barn could be maintained within the range of the thermoneutral zone during winter, primarily to increase feed conversion and to reduce GHG emissions. For this reason, an automation system was installed at a sheep barn in northern Greece, and heat losses from the building were calculated. The biogas potential of the sheep barn waste was examined in the laboratory via the BMP method. The results showed that the installation of an automation system together with a hypothetical biogas heating system could maintain the barn’s temperature in the range of a sheep’s thermoneutral zone during winter for the 94% of the scenarios examined if the total energy of the biogas was utilized, while heating energy that was instantly and continuously used succeeded in 48% of the investigated cases. The surplus of energy produced by biogas could potentially raise the water temperature that animals drink up to 2.9 °C. The absence of cold stress decreases the dry matter intake and the CH4 produced by ruminal fermentation. Moreover, lower GHG emissions are achieved as waste is treated through anaerobic digestion, which would likely be released into the environment if left untreated.
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Addison, Janci D., Evan J. Peterson, and Lyndsi Meyenburg. "Intravenous or Oral Acetazolamide for Treatment of Diuretic-Induced Alkalosis in Patients With Heart Failure." Annals of Pharmacotherapy, February 20, 2023, 106002802311546. http://dx.doi.org/10.1177/10600280231154603.
Повний текст джерелаАнотація:
Background: Acetazolamide has been used for diuretic-induced metabolic alkalosis, but the preferred dose, route, and frequency of administration remain unknown. Objective: The purpose of this study was to characterize dosing strategies and determine the effectiveness of intravenous (IV) and oral (PO) acetazolamide for patients with heart failure (HF) with diuretic-induced metabolic alkalosis. Methods: This was a multicenter, retrospective cohort study comparing the use of IV versus PO acetazolamide in patients with HF receiving at least 120 mg of furosemide for the treatment of metabolic alkalosis (serum bicarbonate CO2 ≥32). The primary outcome was the change in CO2 on the first basic metabolic panel (BMP) within 24 hours of the first dose of acetazolamide. Secondary outcomes included laboratory outcomes, such as change in bicarbonate, chloride, and incidence of hyponatremia and hypokalemia. This study was approved by the local institutional review board. Results: IV acetazolamide was given in 35 patients and PO acetazolamide was given in 35 patients. Patients in both groups were given a median of 500 mg of acetazolamide in the first 24 hours. For the primary outcome, there was a significant decrease in CO2 on the first BMP within 24 hours after patients received the IV acetazolamide (−2 [interquartile range, IQR: −2, 0] vs 0 [IQR: −3, 1], P = 0.047). There were no differences in secondary outcomes. Conclusion and Relevance: IV acetazolamide resulted in significantly decreased bicarbonate within 24 hours of administration. IV acetazolamide may be preferred to treat diuretic-induced metabolic alkalosis in patients with HF.
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Choden, Yeshi, Samten Zangmo, Saahin Tamang, Thinley Gyeltshen, Karma Phuntshok, Sanjita Limboo, and Tenzin Choden Gyeltshen. "Biochemical Methane Potential Assessment by Anaerobic Digestion of Locally Available Grasses of Phuentsholing, Bhutan." Journal of Engineering Research and Reports, April 28, 2021, 135–44. http://dx.doi.org/10.9734/jerr/2021/v20i517320.
Повний текст джерелаАнотація:
Renewable energy is not only environmental friendly but also promotes sustainable development. Biogas being one of the abundantly used renewable resource, the enhancement and optimization of the yield of biogas can help in reduction of dependence on imported fuel. Biochemical methane potential (BMP) assessment of grass will determine the production of methane (CH4) from this substrate through the process of anaerobic digestion. After determining the parameters such as pH, Biochemical Oxygen Demand (BOD) and Total solids (TS) of three types of local grasses known as Basil, Bermuda and Napier, that affects the production of biogas, Napier grass resulted with the highest potential to produce CH4 gas. Batch and continuous reactor method under mesophilic condition was adopted. The composition of biogas from continuous reactor was obtained using a biogas analyzer (Biogas 5000 Geotech), from which 30.8% of CH4,
8% of CO2 and other inert gases were found. Also, methane to carbon dioxide (CH4: CO2) ratio of 3.81: 1 approximately (80% - 20%) was achieved. Moreover, the batch reactor method showed that 1L Napier grass silage would yield 0.81L of biogas. The concentration of CH4 gas from Napier grass in hydraulic retention time as short as 20 days was very significant. This study shows that Napier grass can be used as an alternative sustainable source of energy in the country which can improve resource constraints.
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"Investigation of Solid Waste Characteristics in Field-Scale Landfill Test Cells." Issue 2 (In progress) 21, no. 2 (January 23, 2019): 153–62. http://dx.doi.org/10.30955/gnj.002982.
Повний текст джерелаАнотація:
<p>This paper presents the results of an experimental study carried out in field-scale test cells in order to determine the effect of aeration and leachate recirculation on waste decomposition rate, solid waste characteristics, landfill gas composition and settlement in the landfill body. Four landfill test cells with the dimensions of 20 m x 40 m x 5 m were constructed in Komurcuoda Sanitary Landfill, Istanbul. Solid wastes representing Istanbul Asian side waste characteristics were landfilled in the test cells and they were operated simulating anaerobic (AN1), leachate recirculated anaerobic (AN2), semi-aerobic (A1) and aerobic landfilling (A2) methods. Alternative landfilling methods for accelerating solid waste stabilization in landfills were investigated by means of solid waste characteristics (elemental analysis, pH, moisture content, TOC, TKN, C/N ratio, volatile solid content (VS), biochemical methane potential (BMP), and stability index (SI) analysis), landfill gas components (CH4, CO2, O2, and H2S), temperature variations in landfill body, and landfill settlement. The study indicated that aeration and leachate recirculation accelerate biodegradation rate. Higher rates of MSW biodegradation eventually provide reduction in the contaminant life span of the landfill by achieving a high waste volume reduction in a relatively short duration than anaerobic test cells, decrease the cost of long term monitoring incurred with post-closure of landfill sites. In case of impossibility of aerobic landfilling based on the results of the cost benefit analysis, it was stated out that semi-aerobic landfilling technology is also a viable method in shortening the stabilization time and accelerating the landfill gas production.</p>
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Lundberg, Allie L., Nicole M. Jaskiewicz, Abigail M. Maucieri та David H. Townson. "Stimulatory effects of TGFα in granulosa cells of bovine small antral follicles". Journal of Animal Science 100, № 7 (1 липня 2022). http://dx.doi.org/10.1093/jas/skac105.
Повний текст джерелаАнотація:
Abstract Intraovarian growth factors play a vital role in influencing the fate of ovarian follicles. They affect proliferation and apoptosis of granulosa cells (GC) and can influence whether small antral follicles continue their growth or undergo atresia. Transforming growth factor-alpha (TGFα), an oocyte-derived growth factor, is thought to regulate granulosa cell function; yet its investigation has been largely overshadowed by emerging interest in TGF-beta superfamily members, such as bone morphogenetic proteins (BMP) and anti-Mullerian hormone (AMH). Here, effects of TGFα on bovine GC proliferation, intracellular signaling, and cytokine-induced apoptosis were evaluated. Briefly, all small antral follicles (3–5 mm) from slaughterhouse specimens of bovine ovary pairs were aspirated and the cells were plated in T25 flasks containing DMEM/F12 medium, 10% FBS, and antibiotic-antimycotic, and incubated at 37 °C in 5% CO2 for 3 to 4 d. Once confluent, the cells were sub-cultured for experiments (in 96-, 12-, or 6-well plates) in serum-free conditions (DMEM/F12 medium with ITS). Exposure of the bGC to TGFα (10 or 100 ng/mL) for 24 h stimulated cell proliferation compared to control (P < 0.05; n = 7 ovary pairs). Proliferation was accompanied by a concomitant increase in mitogen-activated protein kinase (MAPK) signaling within 2 h of treatment, as evidenced by phosphorylated ERK1/2 expression (P < 0.05, n = 3 ovary pairs). These effects were entirely negated, however, by the MAPK inhibitor, U0126 (10uM, P < 0.05). Additionally, prior exposure of the bGC to TGFα (100 ng/mL) failed to prevent Fas Ligand (100 ng/mL)-induced apoptosis, as measured by caspase 3/7 activity (P < 0.05, n = 7 ovary pairs). Collectively, the results indicate TGFα stimulates proliferation of bGC from small antral follicles via a MAPK/ERK-mediated mechanism, but this action alone fails to prevent apoptosis, suggesting that TGFα may be incapable of promoting their persistence in follicles during the process of follicular selection/dominance.
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Narimanpour, Zeinab, Maryam Nazm Bojnordi, and Hatef Ghasemi Hamidabadi. "Spermatogenic differentiation of spermatogonial stem cells on three-dimensional silk nanofiber scaffold." Middle East Fertility Society Journal 27, no. 1 (June 21, 2022). http://dx.doi.org/10.1186/s43043-022-00107-5.
Повний текст джерелаАнотація:
Abstract Background Nano-fibrous scaffolds provide a three-dimensional matrix that guides sufficient orientation of seeded cells similar to a natural niche. In this research, we designed a silk scaffold to improve the differention of mouse spermatogonial stem cells to spermatogenic cell lines. Spermatogonial stem cells were collected from neonatal mouse (2–6 days) testes (n=60) using a two steps mechanical and enzymatic method. Cells were seeded on a silk scaffold and were cultured in Dulbecco’s modified Eagle’s medium, supplemented with 15 % fetal bovine serum and 1000 units/ml leukemia inhibitory factor, and incubated at 32°C in a humidified atmosphere of 5% CO2 in air. SEM technique was done for confirmation of seeding cells. In this study two major groups (i.e., 2D and 3D culture groups) of 30 mice each. Isolated testicular cells from each group were cultured in the absence of silk scaffold or the presence of silk scaffold. For induction of differentiation, seeded cells on a scaffold were exposed to 1 μM and 50 ng/ml BMP-4. The specific spermatogenic genes, e.g.; VASA, DAZL, PLZF, and Piwil2, were assessed via real-time PCR and immunocytochemistry techniques. P values less than 0.05 were assumed significant. All experiments were performed at least three times. Results SEM analysis confirmed the homogeneity of fabricated silk scaffold and average diameter of 450 nm for nanofibers fibers. Silk scaffold induces attachment of SSCs in comparison to the monolayer group. Spermatogonia stem cell colonies were observed gradually after 1 week of culture. Electrospun scaffold supports the differentiation of SSCs to spermatogenic lines. Dates of real-time PCR showed that the expression of meiotic markers, VASA, DAZL, and Piwil2 as related to specific spermatogenic genes, had a significant upregulation in cell-seeded silk scaffold compared to the control group (P < 0.05). Immunocytochemistry founding approved the expression of specific spermatogenic markers; DAZL and PLZF were higher in the experiment group compared to the control (P < 0.05). Conclusion It is concluded silk scaffold induces spermatogenic differentiation of mouse spermatogonial stem cells in vitro.
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Parry, D. L., and P. Evans. "Wastewater and organic waste to bioenergy." Water Practice and Technology 7, no. 4 (December 1, 2012). http://dx.doi.org/10.2166/wpt.2012.100.
Повний текст джерелаАнотація:
Throughout the world, wastewater and organic waste are increasingly being viewed as energy sources and the practice of converting them into bioenergy through conversion to biogas with anaerobic digestion is growing. This paper presents an overview of planning, research, and full-scale operations of both separate and codigestion of organic waste. Organic waste management methods are compared with respect to economic (life-cycle costs), environmental (equivalent carbon dioxide emissions), social, and operational impacts for a representative 100,000 population community. Management methods include using sewers or trucks to transport the organics to anaerobic digesters at a wastewater treatment plant, using a material recovery facility (MRF) to extract the organics from municipal solid waste for anaerobic digestion, composting the organic waste, or sending the organics to a landfill. Hauling the organics to anaerobic digesters had the lowest equivalent CO2 emissions, while using the sewer to convey organics had the lowest life-cycle cost. An example of codigestion of organic waste with wastewater sludge at the Des Moines Water Reclamation Facility (Iowa, USA) is described. The limits of organic loading rates for digestion of FOG (fats, oils, and grease) with wastewater sludge are presented based on research using 1,000-litre (L) pilot digesters. A specific energy loading rate (SELR) is proposed as an improved parameter for organic loading rates. The SELR is a measure of energy loading relative to the reactor biomass, and is an innovative approach to characterizing digester capacity and stability. Food wastes from the cafeteria at the U.S. Air Force Academy were digested in bench-scale, semi-continuous reactors and monitored using an online respirometer capable of continuously monitoring gas flow rate and gas composition. The biological methane potential (BMP) of several organic wastes were measured in lab-scale digesters. Organic wastes were digested with and without domestic wastewater sludge. Separate digestion of organic wastes was found to be nutrient (cobalt, nickel) deficient, where codigestion with wastewater sludge experienced no deficiencies. Codigestion could also handle a greater amount of FOG being fed to the digesters than separate digestion of food wastes.