Добірка наукової літератури з теми "Clinico-chemical parameters"

The effects of repeated oral administration of clarithromycin (7.5 mg/Kg B.W., daily for 5 consecutive days) on blood parameters, clinico-chemical parameters and vital organ histology were evaluated in Ross broiler chickens. Twelve clinically healthy birds have been used in a parallel study design, where they have been randomly divided into two groups. The 1st group received a dose of 7.5 mg clarithromycin/Kg as intracrop bolus daily for 5 consecutive days after 6 h fasting; while the 2nd group received iso-saline and was kept as control. Blood samples were collected from all birds via the wing and metatarsal vein punctures on the 5th day for clinico-chemical and haematological examinations; and liver, kidneys and heart were dissected out for histopathological examination. Results revealed that repeated administration of clarithromycin caused significant increases in ALP, AST, ALT, CK, urea and creatinine levels, compared to those of control. Metabolic parameters exhibited significant decrease in albumin with consequent decrease in total protein and decreased albumin/globulin ratio. Lipid parameters remained unchanged, compared to control. Leuckogram revealed that total leuckocytic count was decreased as a result of the decreased number of lymphocytes and mid-sized cells. On the other hand, granulocytes number exhibited significant increase (granulocytosis) together with increased number of platelets (thrombocytosis); while erythrogram remained insignifi-cantly affected. Liver and kidney showed inflammatory cellular infiltrations associated with degenerative changes and haemorrhages. Although heart did not show inflammatory cellular infiltrations, yet breakage in some bundles was noted. These data indicate that although clarithromycin did not cause any clinical serious manifestations on the treated birds, yet laboratory analysis revealed some adverse effects on some organs as liver, kidney and heart as well as alterations in some clinic-chemical and hematological parameters; these findings should be taken in consideration during therapy with clarithromycin.

7049 Background: Bone metastases (BM) in non small cell lung cancer (NSCLC) may be present at diagnosis or develop in the follow up, are associated with a worse prognosis, and currently there are no chemical or biological markers predicting their clinical onset. Methods: Thirty cases of resected NSCLC which subsequently develop BM (group A - mean follow up time 27.2 months) were matched for several clinico-pathological parameters (including age, sex, stage of the disease, histology, differentiation grade, adjuvant therapy) to 30 cases of resected NSCLC without any metastases (group B - mean follow up time 75.1 months) and 26 resected NSCLC with non-bone metastatic (group C - mean follow up 21.1 months). Primary tumor samples were investigated by immunohistochemistry for 10 markers previously recognized to be involved in bone resorption or metastatization process (cathepsin K, bone sialoprotein [BSP], VEGF, MMP-2, p53, RECK, TIMP-1, CD-117, Ki-67 and TRAcP). For statistical analysis the intensity of the staining was assessed by a semi-quantitative score (0, <10%, 10–50%, >50% +ve tumor cells). Differences among groups were estimated by X-square test, whereas the prognostic impact of clinico-pathological parameters and markers expression was evaluated by univariate and multivariate analyses. Results: Among the different markers investigated, BSP was strongly associated to bone dissemination (p < 0.001) and, independently, to poor outcome (p = 0.02 by Mantel-Cox test). None of the other markers was differentially expressed within the groups or demonstrated a prognostic impact, both in terms of overall survival and of time interval to metastases. Based on these findings, the prevalence of BSP in NSCLC was further estimated in a large series of 120 resected lung carcinomas (M:F ratio 3:1; mean age 67 years; adenocarcinomas 55%, squamous cell carcinoma 39%, others 6%; stages: I 54%, II 17%, III 29%). BSP prevalence reached 40%, without any statistically significant difference according to histotype or other clinico-pathological parameters. Conclusions: BSP protein expression in resected NSCLC strongly predicts bone dissemination, and may therefore be useful in selecting patients for treatments targeted to inhibit bone metastatic spread. [Table: see text]

The role of vitamin D was well established in bone metabolism as well as in the inflammatory process of a disease. The periodontal disease which is a chronic inflammatory condition with destructive bone metabolism and type II diabetes mellitus which is a metabolic condition with more prone to inflammatory reactions are prevalent in middle-aged patients. Hence the present study is done to assess the vitamin D levels in serum of chronic generalized periodontitis patients with type2 Diabetes mellitus and simple gingivitis patients Two groups with 50 patients of both periodontal disease with type 2 diabetes and 50 simple gingivitis patients of age 35 to 55 years old were recruited in this study. For all the patient's serum 25(OH)D levels, oral hygiene index (OHI), Russel’s periodontal index, and presence of bony defects in radiographs were assessed.After assessment of all the parameters patients with periodontal disease and type 2 diabetes mellitus showed significantly higher proportion levels than the simple gingivitis patients and significantly lower levels in 25(OH)D (&#60;From the results, it was concluded that generalized periodontitis patients with type II diabetes mellitus showed higher levels of 25-hydroxyvitamin D levels than simple gingivitis patients.

AbstractVarious tools have been developed over previous years to study the welfare of laboratory animals. These include preference tests, which are commonly used to evaluate housing environments. Preference tests, however, have some pitfalls: they supply information only on the animals’ present preferences, and they allow the animal the choice only between the options offered. Other methods based upon the collection of clinico-chemical data require handling of the animals, which can be stressful in itself. An alternative may be to use telemetry to measure the changes in physiological parameters caused by different environmental conditions. The aim of this study was to use telemetry to evaluate the short-term impact of housing conditions on rodents. We monitored heart rate, blood pressure and body temperature in rats kept on three different types of flooring — bedding, grid floors and plastic floors. The study revealed significant differences in systolic and diastolic blood pressure, heart rate and body temperature between rats housed in the three conditions, indicating that both grid floors and plastic floors are more stressful for the animals than bedding. The observed differences did not diminish over the two-week observation period. The grid-floor housing induced elevations in blood pressure and heart rate. Blood pressure remained elevated even when the animals were returned to standard bedding, whereas the heart rate declined back to its original value immediately in response to this shift. This study shows that telemetry is a very effective tool but that it needs integrating with other methods: in addition, a greater understanding of the biological significance of the changes in cardiovascular parameters is required before the hypothesis that these changes represent an indication of distress can be accepted.

Background Patients with end-stage renal disease (ESRD) undergoing hemodialysis (HD) are apparently exposed to enhanced oxidative stress and to inflammation. It was the aim of this study to characterize the state of systemic oxidative stress of ESRD patients before and following HD using highly specific biomarkers, F2-isoprostanes and 4-hydroxynonenal (HNE). Furthermore the question should be answered, if there are associations between inflammation and systemic oxidative stress and/or between systemic oxidative stress and renal anemia, which is more or less typical for HD patients. Patients and methods Concentrations of F2-isoprostanes, HNE, C-reactive protein (CRP) as marker of inflammation, and hemoglobin were measured in serum samples of patients with ESRD before and after HD and of healthy control persons for comparison. Total (esterified plus free) F2-isoprostanes were quantified by highly sensitive gas chromatography/mass spectrometry technique, HNE by thin layer chromatography and HPLC/UV detection, CRP by immunoturbidimetry and hemoglobin by clinico-chemical routine assay. Results 1. HD patients showed significantly higher serum concentrations of F2-isoprostanes and HNE than healthy human control subjects. 2. Total (esterified plus free) F2-isoprostane levels before HD were not significantly different from those after HD, whereas HNE levels were significantly decreased in patients after HD. 3. F2-isoprostane concentrations in HD patients correlated with the levels of CRP, whereas HNE concentrations inversely correlated with the content of hemoglobin. Conclusion Both, F2-isoprostanes and HNE serum concentrations are useful oxidative stress parameters in ESRD patients undergoing HD. Whereas HNE strongly correlates with the severity of renal anemia, leading to left heart insufficiency, F2-isoprostanes (sum of free plus esterified) highly correlate with the degree of inflammation.

Nuclear magnetic resonance (NMR) imaging is an established diagnostic medium to diagnose multiple sclerosis (MS). In clinically stable MS patients, NMR detects silent disease activity, which is the reason why it is being used to monitor treatment trials, in which it serves as a secondary outcome parameter. The absence of a clear correlation with clinical disability, the so–called ‘clinico–radiological’ paradox, and the poor predictive value of NMR prohibit the use of NMR as a primary outcome parameter in clinical trials. This is––among others––a result of the limited histopathological specificity of conventional, or ‘T2–weighted‘ imaging, the most commonly used NMR technique. In this paper we review additional NMR techniques with higher tissue specificity, most of which show marked heterogeneity within NMR–visible lesions, reflecting histopathological heterogeneity. Gadolinium enhancement identifies the early inflammatory phase of lesion development, with active phagocytosis by macrophages. Persistently hypointense lesions on T1–weighted images (‘black holes’) relate to axonal loss and matrix destruction, and show a better correlation with clinical disability. Marked prolongation of T1 relaxation time correlates with enlargement of the extracellular space, which occurs as a result of axonal loss or oedema. Axonal viability can also be measured using the concentration of N –acetyl aspartate (NAA) using NMR spectroscopy; this technique is also capable of showing lactate and mobile lipids in lesions with active macrophages. The multi–exponential behaviour of T2 relaxation time in brain white matter provides a tool to monitor the myelin water component in MS lesions (short T2 component) as well as the expansion of the extracellular space (long T2 component). Chemical exchange with macromolecules (e.g. myelin) can be measured using magnetization transfer imaging, and correlates with demyelination, axonal loss and matrix destruction. Increased water diffusion has been found in MS lesions (relating to oedema and an expanded extracellular space) and a loss of anisotropy may indicate a loss of fibre orientation (compatible with demyelination). Apart from the histopathological heterogeneity within focal MS lesions, the normal–appearing white matter shows definite abnormalities with all quantifiable NMR techniques. A decrease in the concentration of NAA, decreased magnetization transfer values and prolonged T1 relaxation time values are probably all related to microscopic abnormalities, including axonal damage. This ‘invisible’ lesion load may constitute a significant proportion of the total lesion load but is not visible on conventional NMR. Similarly, mechanisms for clinical recovery exist, which are not distinguished using MR imaging. Therefore, it is highly unlikely that the clinico–radiological paradox will ever be solved completely. However, NMR provides an opportunity to sequentially measure tissue changes in vivo . Using MR parameters with (presumed) histopathological specificity, the development of (irreversible) tissue damage can be monitored, which perhaps allows the identification of factors that determine lesional outcome in MS. Since the absence of severe tissue destruction is prognostically favourable, NMR monitoring of the extent to which such changes can be prevented by treatment will ultimately benefit the selection of future treatment strategies.

Background: Myriad of synthetic products has been used in chemical plaque control. There is a constant search for cost effective herbal products with minimal adverse effect to substitute synthetic compounds. The objective of this study was to evaluate the anti-inflammatory properties of Cocos nucifera and Sesamum indicum and compare their effect with commercially available chlorhexidine on gingivitis. Methods: In this double blind, randomized, control clinical trial, a total of 45 samples from patients aged between 18 to 35 years reporting to the institution, diagnosed with gingivitis were selected and randomly divided into Group-A (Scaling + Cocos Nucifera mouthwash), Group-B (Scaling + Sesamum Indicum mouthwash) and Group C (Scaling + Chlorhexidine mouthwash). Clinical (Plaque index, Gingival index and Sulcus bleeding index), and Immunological (Interleukin-6) parameters were assessed at baseline and 45th day following scaling. Saliva samples were collected and stored at -200C till they were subjected to enzyme-linked immunosorbent assay (ELISA) analysis. Inferential statistics done were analysis of variance, paired t test, post hock Scheffe test and Chi- square test by using SPSS software (22.0). Results: In Clinical parameters, group B (p˂0.001) showed statistical significant reduction compared to group A and group C. In Immunological parameter group A (p˂0.001) showed statistical significant reduction in Interleukin-6 compared to group B and group C (p=0.126 & p=0.196 respectively). Conclusion: Cocos nucifera and Sesamum indicum mouth washes effectively decreased plaque formation and could be used as an adjunct to scaling in treating plaque induced gingivitis.

AbstractN-Acetyl-L-cysteine (NAC) is an endogenous cysteine metabolite. The drug is widely used in chronic obstructive pulmonary disease (COPD) and as antidote in acetaminophen (paracetamol) intoxication. Currently, the utility of NAC is investigated in rheumatoid arthritis (RA), which is generally considered associated with inflammation and oxidative stress. Besides clinical laboratory parameters, the effects of NAC are evaluated by measuring in plasma or serum nitrite, nitrate or their sum (NOx) as measures of nitric oxide (NO) synthesis. Malondialdehyde (MDA) and relatives such as 4-hydroxy-nonenal and 15(S)-8-iso-prostaglandin F2α serve as measures of oxidative stress, notably lipid peroxidation. In this work, we review recent clinico-pharmacological studies on NAC in rheumatoid arthritis. We discuss analytical, pre-analytical and clinical issues and their potential impact on the studies outcome. Major issues include analytical inaccuracy due to interfering endogenous substances and artefactual formation of MDA and relatives during storage in long-term studies. Differences in the placebo and NAC groups at baseline with respect to these biomarkers are also a serious concern. Modern applied sciences are based on data generated using commercially available instrumental physico-chemical and immunological technologies and assays. The publication process of scientific work rarely undergoes rigorous peer review of the analytical approaches used in the study in terms of accuracy/trueness. There is pressing need of considering previously reported reference concentration ranges and intervals as well as specific critical issues such as artefactual formation of particular biomarkers during sample storage. The latter especially applies to surrogate biomarkers of oxidative stress, notably MDA and relatives. Reported data on NO, MDA and clinical parameters, including C-reactive protein, interleukins and tumour necrosis factor α, are contradictory in the literature. Furthermore, reported studies do not allow any valid conclusion about utility of NAC in RA. Administration of NAC patients with rheumatoid arthritis is not recommended in current European and American guidelines.

Prenatal growth is influenced by bidirectional exchange between the fetal and maternal systems that programme birth weight and postnatal phenotype. Fetal fluids are essential but unexplored areas for prenatal growth. This study examined allantoic and amniotic fluids and maternal blood in a Bos taurus, Bos indicus bovine model to provide fundamental information that is lacking regarding (i) changes in clinico-chemical parameters of maternal blood in early and midgestation and their relationships with placental-embryo/fetal weights (ii) differences in clinico-chemical parameters of allantoic and amniotic fluids of bovine concepti in early and midgestation, (iii) effects of genetics and sex on clinico-chemical parameters of fetal fluids and their relationships with feto-placental phenotype, and (iv) the effects of conceptus sex and genetics on maternal blood parameters. Purebred and reciprocal cross Bos taurus, (Bt) and Bos taurus indicus, (Bi) concepti were recovered in early gestation (Day 48, n = 60), and midgestation (Day 153, n = 72), (term ~ 280 days). Fetal fluids and maternal blood were sampled and analysed for clinico-chemical parameters. Insulin-like growth factors and thyroid hormones were assessed in maternal blood. Effects of genetics and sex on the measured parameters were determined using general linear models, and relationships with feto-placental phenotype assessed by linear regression. Breed effects on maternal electrolytes, metabolites and enzymes and their relationships with placental-embryo/fetal weights provide evidence of differences in maternal mineral metabolism, liver and kidney functions that influence conceptus growth. Stage- and genetic-specific differences were seen in clinico-chemical parameters of fetal fluids, which likely reflect differences in feto-placental phenotype. Greater maternal genome effects on fetal fluid in early gestation reflect the significance of placenta and maternal environment and represent maternal-offspring coadaptation. Paternal genome and maternal by paternal genome interactions exert stronger effects on amniotic fluid suggesting greater fetal influence and (epi)genetic regulation in line with the conflict-of-interest hypothesis. Conceptus sex had strong effects on fetal fluid metabolites in early gestation, while parental genome by sex effects influence amniotic fluid parameters at both stages. Furthermore, paternal genome and sex interacted with non-genetic maternal effects to affect fetal fluid parameters at both stages. Significant relationships between maternally controlled allantoic fluid parameters and embryo-placental weights suggest a critical role of allantoic fluid for embryonic development. In midgestation, paternally controlled amniotic fluid Na/K ratio showed significant relationships with fetal weight and fetal fluid volume, suggesting a paternal influence on amniotic fluid water and nutrient transfer. Conceptus sex and genetics affected maternal physiology in a maternal genetics dependent manner by influencing key parameters of maternal mineral metabolism, liver function and thyroid status. In conclusion, this study provides reference values for clinico-chemical parameters of fetal fluids and maternal serum in Bt and Bi concepti in early and midgestation. Results support the hypothesis that fetal fluid parameters are affected by genetics and sex, and are related to feto-placental phenotype, demonstrating their critical role in prenatal growth. This study highlights the importance of considering conceptus sex, genetics and maternal genetics as factors that impact maternal physiology and demonstrates the need for genetic background-specific maternal assessment.
Thesis (Ph.D.) -- University of Adelaide, School of Animal and Veterinary Sciences, 2020