Дисертації з теми "CLBP"
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Dubois, Damien. "L'ilot génomique pks chez Escherichia coli : structure-fonction de la protéine ClbP et études épidémiologiques." Phd thesis, Université d'Auvergne - Clermont-Ferrand I, 2011. http://tel.archives-ouvertes.fr/tel-00612631.
Повний текст джерелаMukandoli, Kumuntu. "Predisposing factors of chronic low back pain (CLBP) among sedentary office workers (SOW) in Nairobi, Kenya." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&.
Повний текст джерелаto determine the possible predisposing factors of chronic low back pain and to determine the impact of chronic low back pain on work related quality of life among sedentary office workers in Nairobi, Kenya.
Snelgrove, Sherrill. "A longitudinal investigation into patients' experiences of chronic low back pain (CLBP) using interpretative phenomenological analysis (IPA)." Thesis, Swansea University, 2010. https://cronfa.swan.ac.uk/Record/cronfa42594.
Повний текст джерелаGottwald, Cornelia Renate Verfasser], Harald [Akademischer Betreuer] [Gutachter] [Walach, and Stefan [Gutachter] Schmidt. "Chronischer Schmerz des unteren Rückens (CLBP)-ein medizinisches Leitsymptom unserer Kultur und eine zeitgemäße Behandlungsmöglichkeit / Cornelia Renate Gottwald ; Gutachter: Harald Walach, Stefan Schmidt ; Betreuer: Harald Walach." Frankfurt (Oder) : Europa-Universität Viadrina Frankfurt, 2017. http://d-nb.info/1142769844/34.
Повний текст джерелаCampos, Lara. "Predição da Resposta de Sucesso a um Programa de Exercício em Meio Aquático para Utentes com Dor Lombar Crónica." Master's thesis, Instituto Politécnico de Setúbal. Escola Superior de Saúde, 2014. http://hdl.handle.net/10400.26/7683.
Повний текст джерелаPALAVRAS-CHAVE: Dor Lombar Crónica (DLC), Factores de Prognóstico, Intensidade da Dor, Incapacidade Funcional Introdução e Objectivo: A evidência existente acerca de potenciais factores que possam predizer resultados de sucesso em utentes com DLC é não só escassa, mas sobretudo pouco consistente. O presente estudo teve como objectivo identificar factores de prognóstico para os bons resultados da Fisioterapia, a curto e médio prazo, ao nível da intensidade da dor, capacidade funcional e percepção de melhoria em indivíduos com DLC, que realizaram um programa de exercício em meio aquático. Metodologia: Foi realizado um estudo de coorte prospectivo, com 42 participantes com DLC; os quais foram submetidos a um programa de exercício aquático, com duração de 6 semanas. Os resultados do programa foram avaliados imediatamente após o seu término, e três meses após o final do tratamento. Os outcomes de interesse foram a intensidade da dor, medida pela Escala Visual Analógica (EVA), a incapacidade funcional, medida pela Quebek Back Pain Disability Scale – Versão Portuguesa (QBPDS-PT), e a percepção global de melhoria, medida pela Patient Global Impression Change Scale – Versão Portuguesa (PGIC-PT). As características sociodemográficas e clínicas avaliadas no início do estudo foram incluídas como potenciais factores de prognóstico. Como critérios de sucesso, foram utilizadas as Diferenças Clínicas Minimamente Importantes (DCMIs) definidas na literatura para os três instrumentos utilizados. Resultados: Os resultados obtidos sugerem que: 1) ao nível da intensidade da dor, as variáveis de prognóstico intensidade da dor reportada na baseline (OR= 1,049; 95% IC 1,004-1,097) e presença de irradiação para o membro inferior (OR=13,418; 95% IC 1,963- 91,716) estão significativamente associadas com os resultados de sucesso imediatamente após o programa de exercício aquático (6 semanas); e a intensidade da dor reportada na baseline está significativamente associada com os resultados de sucesso, três meses após o final do tratamento (OR=1,045; 95% IC 1,004-1,089); 2) ao nível da incapacidade funcional, apenas a pontuação na QBPDS-PT reportada na baseline se encontra estatisticamente associada com a incapacidade funcional registada 6 semanas após o início do estudo (OR=1,061 95% IC 1,009-1,115). Conclusões: Utentes com níveis mais elevados de intensidade de dor e presença de irradiação da dor para o membro inferior, no início do estudo, apresentam maior probabilidade de sucesso, ao nível da intensidade da dor, imediatamente após um programa de exercício aquático; e utentes com maiores níveis de intensidade da dor, no início do estudo, apresentam maior probabilidade de sucesso, também ao nível da intensidade da dor, três meses após o final do tratamento. Utentes com maiores níveis de incapacidade funcional no início do estudo, apresentam maior probabilidade de atingirem resultados de sucesso, ao nível da incapacidade funcional, imediatamente após o final do programa de exercício aquático.
Abstract:Introduction and Objectives: There is little evidence about potential prognostic factors that can influence the successful outcomes of patients with CLBP. The aim of this study was to assess prognostic factors for the success of an aquatic exercise program, for pain intensity, disability and global impression change, in patients with CLBP; immediately after the treatment and in a 3 months follow-up. Methodology: It was used a prospective cohort study with 42 participants, who undertake an exercise aquatic program for 6 weeks. The results of the program were assessed immediately after the treatment, and at 3 months follow-up. The primary outcomes were pain intensity, measured by Visual Analogic Scale (VAS), functional disability, measured by Quebek Back Pain Disability Scale – Portuguese Version (QBPDS-PT), and the global impression of change, measured by the Patient Global Impression Change Scale – Portuguese Version (PGIC-PT). The socio-demographic and clinical data were used as potential prognostic factors. Success with the treatment was defined considering the Minimal Clinically Important Difference (MCID) reported on the literature for the three instruments used. Results: For pain intensity, the variables pain intensity in the baseline (OR= 1,049; 95% IC 1,004-1,097) and presence of irradiating pain (OR=13,418; 95% IC 1,963-91,716), were associated with successful results for pain intensity, immediately after the end of the exercise aquatic program; and the pain intensity in the baseline were associated with successful results in the 3 months follow-up (OR=1,045; 95% IC 1,004-1,089). In what concerns to functional disability, only the score obtained in the QBPDS-PT, at the baseline, was associated with the functional disability assessed immediately after the end of the treatment (OR=1,061 95% IC 1,009-1,115). Conclusions: Participants with more pain intensity and presence of irradiating pain in the baseline, were more associated with results of success in pain intensity, immediately after a program of aquatic exercise; and participants with more pain intensity, in the baseline, were more probably associated with results of success in pain intensity, at the follow-up of 3 months. Participants with more disability at the baseline were more likely to present successful results, in functional disability, immediately after the end of the treatment.
Infante, Meza María Soledad. "Clip." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101664.
Повний текст джерелаMontero, Prieto María del Rosario. "Clip." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101408.
Повний текст джерелаKholodko, S. G. "Paper clip." Thesis, Сумський державний університет, 2014. http://essuir.sumdu.edu.ua/handle/123456789/34864.
Повний текст джерелаBocquet, Frédéric. "Remplissage des colonnes CLHP." Paris 5, 1996. http://www.theses.fr/1996PA05P010.
Повний текст джерелаFinke, Melanie [Verfasser], and Markus [Akademischer Betreuer] Bischoff. "Einfluss der HSP100/Clp ATPasen ClpB und ClpC aus Staphylococcus aureus auf die Internalisierung und das Langzeitüberleben in eukaryoten Zellen / Melanie Finke. Betreuer: Markus Bischoff." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2015. http://d-nb.info/1064868606/34.
Повний текст джерелаGolumbeanu, Monica. "Statistical Analysis of PAR-CLIP data." Thesis, KTH, Beräkningsbiologi, CB, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-124347.
Повний текст джерелаWright, David. "Disentangling an unfoldase : structural studies of ClpB." Thesis, Birkbeck (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.504736.
Повний текст джерелаLanghorst, Hanna [Verfasser]. "Deletion of Clmp in Mice Reveals Essential Roles for CLMP in Growth, Survival, Gastrointestinal and Urinary Tract Functions / Hanna Langhorst." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1084634678/34.
Повний текст джерелаTibbles, Katherine L. "Regulation of Clb1 during meiosis in Saccharomyces cerevisiae." Thesis, University of Warwick, 2013. http://wrap.warwick.ac.uk/60444/.
Повний текст джерелаMawla, Gina D. (Gina Danielle) Ph D. Massachusetts Institute of Technology. "Functions of alternative ClpP subunits in Pseudomonas aeruginosa." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127135.
Повний текст джерелаCataloged from the official PDF of thesis.
Includes bibliographical references.
Proteolysis is the process by which proteins are broken down, or hydrolyzed, into small peptides or amino acids by enzymes. Cells from all forms of life carry out regulated protein degradation as a way to control cellular physiology and regulate stress responses. Clp proteases, containing a AAA+ (A̲TPases A̲ssociated with various cellular A̲ctivities) unfoldase stacked with a compartmentalized peptidase, are central to bacterial proteolysis, and use the energy of ATP hydrolysis to unfold and translocate protein substrates into the peptidase chamber for their destruction. The opportunistic pathogen Pseudomonas aeruginosa is unusual in that it contains two isoforms of the subunits that form the ClpP peptidase chamber. These isoforms, PaClpP1 and PaClpP2, have not been well characterized previously and their specific functions are largely elusive. This work examines the structures and functions of PaClpP1 and PaClpP2 and proposes a model for functional peptides generated by these enzymes in P. aeruginosa development. Biochemical analysis establishes that PaClpP2 is only active as a peptidase when it is part of a PaClpP1₇P2₇ heterocomplex. Furthermore, multiple lines of evidence support that P. aeruginosa cells have two distinct ClpP peptidase assemblies: PaClpP1₁₄ and PaClpP1₇P2₇. Importantly, peptidase and protease analyses establish that these two ClpP assemblies exhibit distinct peptide cleavage specificities and interact differentially with the AAA+ unfoldases, ClpX and ClpA. Finally, the PaClpP2 peptide-cleavage active site uniquely contributes to P. aeruginosa biofilm development. Therefore, results presented in this thesis suggest that within AAA+ proteases, the specificity of the peptidase subunits, not only the recognition properties of the AAA+ unfoldase, control the biological outcome(s) of proteolysis.
by Gina D. Mawla.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Biology
GAO, XIAOJIANG. "STRENGTH DETERMINATION OF HEAVY CLIP-ANGLE CONNECTION COMPONENTS." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1134401462.
Повний текст джерелаCompise, Karin D. "Student perceptions of the Clip Chart Management System." Scholarly Commons, 2019. https://scholarlycommons.pacific.edu/uop_etds/3625.
Повний текст джерелаZheng, Bo. "Characterisation of the Clp Proteins in Arabidopsis thaliana." Doctoral thesis, Umeå : Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-99.
Повний текст джерелаGersch, Malte [Verfasser]. "Structure, Function and Inhibition of ClpP Proteases / Malte Gersch." München : Verlag Dr. Hut, 2014. http://d-nb.info/1067707883/34.
Повний текст джерелаParakh, Neville J. "Design and implementation of a ferry clip test system." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27613.
Повний текст джерелаScience, Faculty of
Computer Science, Department of
Graduate
Hersch, Greg Louis. "ClpX interactions with ClpP, SspB, protein substrate and nucleotide." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/34199.
Повний текст джерелаIncludes bibliographical references.
ClpXP and related ATP-dependent proteases are implements of cytosolic protein destruction. They couple chemical energy, derived from ATP hydrolysis, to the selection, unfolding, and degradation of protein substrates with the appropriate degradation signals. The ClpX component of ClpXP is a hexameric enzyme that recognizes protein substrates and unfolds them in an ATP-dependent reaction. Following unfolding, ClpX translocates the unfolded substrate into the ClpP peptidase for degradation. The best characterized degradation signal is the ssrA-degradation tag, which contains a binding site for ClpX and an adjacent binding site for the SspB adaptor protein. I show that the close proximity of these binding elements causes SspB binding to mask signals needed for ssrA-tag recognition by ClpX. The SspB dimer overcomes this signal masking by tethering itself and bound substrate to ClpX, via docking sites located in the dimeric N-terminal domain of ClpX. Because this N-domain dimer binds only a single SspB subunit, the ClpX hexamer can accommodate just one SspB dimer per hexamer. Other adaptor proteins that use these same tethering sites must compete with SspB for access to ClpXP. Substrates bearing ssrA tags with increased spacing between the SspB and ClpX binding elements are degraded more efficiently at low concentrations by ClpXP.
(cont.) This mechanism in which the adaptor first obstructs and then stimulates substrate recognition may have evolved to permit an additional level of regulation of substrate choice. SspB binding to ssrA-tagged substrate is a highly dynamic process, allowing rapid transfer of substrates from SspB to ClpX. Although the ClpX hexamer is composed of six identical polypeptides, individual subunits assume at least three distinct conformations. Using a hexamer that was engineered to prevent nucleotide hydrolysis, I show that some nucleotide-binding sites in ClpX release ATP rapidly, others release ATP slowly, and at least two sites remain nucleotide free. Occupancy of both the slow sites by ATP and the fast sites by either ATP or ADP is required to bind the degradation tags of protein substrates. The ability of ClpX to retain binding of substrate with ATP or ADP in the fast sites suggests that nucleotide hydrolysis in the fast sites, but not in the slow sites, will allow repeated unfolding attempts without substrate release over multiple ATPase cycles. My results rule out ATPase models including ClpX6eATP6 or ADP6 and also suggest that the enzyme hydrolyzes only a fraction of bound ATP in a single turnover event. Short peptide motifs of ClpX, known as IGF loops, interact with ClpP and change conformation as a response to nucleotide binding by ClpX.
(cont.) As ClpX varies its nucleotide content during the ATP hydrolysis cycle, it also varies its affinity for ClpP. Processing of substrates is coupled to the ATP-hydrolysis cycle of ClpX and appears to modulate ClpX's affinity for ClpP by changing how long each ClpX subunit spends in each nucleotide state.
by Greg Louis Hersch.
Ph.D.
Zhang, Ting. "Mechanism of aggregate reactivation by the molecular chaperone CLPB." Diss., Kansas State University, 2012. http://hdl.handle.net/2097/13628.
Повний текст джерелаGraduate Biochemistry Group
Michal Zolkiewski
ClpB, a bacterial chaperone that belongs to the AAA+ protein family, cooperates with the Hsp70/40 system (DnaK, DnaJ and GrpE in E.coli) in the reactivation of aggregated substrates by translocating them through the central channel of its hexameric form. ClpB is essential for survival of bacteria under heat shock and plays an important role in the infectivity of pathogenic microorganisms. However the detailed mechanism of ClpB disaggregation activity is still not clear. ClpB is a multi-domain protein, which consists of two nucleotide binding domains (NBD1 and NBD2) connected by the middle domain (M domain), and the N-terminal domain connected to the rest of the protein by a flexible linker. In this work, mutations were introduced into the linker region to modify the mobility of the N-terminal domain. It was found that without altering the proper folding and oligomerization of ClpB, all the mutants had deficiencies in aggregate reactivation, possibly due to the weaker binding to aggregated substrates in the initial step of disaggregation. This led to the conclusion that the flexible attachment of the N-terminal domain supports substrate binding and controls the disaggregation by ClpB. Moreover, partial inhibition of the ClpB chaperone activity was observed for all the linker variants, suggesting that the linker sequence might have been optimized by selective pressure to maintain the optimal efficiency of aggregate reactivation. To study the substrate translocation of ClpB, a BAP (ClpB-ClpA P-loop) variant that binds to the protease ClpP was constructed. A FRET-based experiment was designed and the fluorescently-labeled ClpB substrates were produced. This work sets the stage for further studies on the mechanism of aggregate recognition by ClpB. ClpB also plays important roles in pathogenic bacteria invasion and virulence. Recombinant ClpB from Ehrlichia chaffeensis, a pathogenic bacterium that causes human monocytic ehrlichiosis, was purified to study its biochemical properties. Ehrlichia ClpB (Eh_B) and E.coli ClpB (Ec_B) sequences are highly conserved in the nucleotide binding region and poorly conserved in the N-terminal and M domain. The oligomerization, ATPase activity, chaperone activity and substrate binding of the recombinant Eh_B were tested. Recombinant Eh_B was able to reactivate aggregated proteins in the presence of HSP70 from E.coli with equal efficiency as Ec_B. However, the mechanism of Eh_B interactions with substrates and/or substrate specificity may be different from that of E. coli ClpB.
Martins, Pedro Filipe M. V. C. "Pesquisa de clip arts combinando imagens raster e vectoriais." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/8450.
Повний текст джерелаFundação para a Ciência e Tecnologia - Projecto CRUSH (Clip art Retrieval using Sketches),referência PTDC/EIA-EIA/108077/2008
Marchiset-Leca, Dominique. "Pirarubicine : dosages par clhp ; etude pharmacocinetique-pharmacodynamie, applications cliniques." Aix-Marseille 2, 1996. http://www.theses.fr/1996AIX22953.
Повний текст джерелаFowler, Shaunda Lynn. "Clip reactions in standing seam roofs of metal buildings." Diss., Mississippi State : Mississippi State University, 2001. http://library.msstate.edu/etd/show.asp?etd=etd-07132001-151614.
Повний текст джерелаSwain, Martin T. "Protein side-chain placement using CLP." Thesis, University of Aberdeen, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248614.
Повний текст джерелаAli, Arslan Mehmet. "Generation and Bioinformatic Analysis of Synthetic Ago HITS-CLIP Data." Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-204891.
Повний текст джерелаPersonne, Yoann. "Role of the two ClpP protease subunits in Mycobacterium tuberculosis." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/2962.
Повний текст джерелаCahagne-Leroux, Isabelle. "Etude de l'énantiosélectivité en CLHP de protéines greffées sur silice." Bordeaux 1, 1990. http://www.theses.fr/1990BOR10542.
Повний текст джерелаJampel, Michael Benjamin. "Over-constrained systems in CLP and CSP." Thesis, City University London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319633.
Повний текст джерелаRhod, Eduardo Luis. "Quaternary CLB a falul tolerant quaternary FPGA." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/72925.
Повний текст джерелаThe decrease in transistor size is increasing the number of functions that can be performed by the electronic devices. Despite this reduction in the transistors minimum size, the circuit’s speed does not follow the same rate. One of the major reasons pointed out by researchers are the interconnections between the transistors and between the components. The increase in the number of circuit interconnections brings a significant increase in energy consumption, propagation delay of signals, and an increase in the complexity and cost of new technologies IC designs. As a possible solution to this problem the use of multivalued logic is being proposed, more specifically, the quaternary logic. FPGA devices are characterized mainly by offering greater flexibility to designers of digital systems. However, with the advance in IC manufacturing technologies and the reduced size of the minimum fabricated dimensions, the problems related to the large number of interconnections are a concern for future technologies of FPGAs. The sub 90nm technologies have a large increase in the error rate of its functions for the combinational and sequential logic. Although potential solutions are being investigated by the community, the search for circuits tolerant to radiation induced errors, without performance, area, or power penalties, is still an open research issue. This work proposes the use of quaternary circuits with modifications to tolerate faults from transient events. The main contribution of this work is the development of a quaternary CLB (Configurable Logic Block) able to withstand transient events and the occurrence of soft errors.
Dominique, Manon. "Effets pharmacologiques d'une protéine bactérienne mimétique d'hormones satiétogènes : la protéine ClpB sur le comportement alimentaire." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR072/document.
Повний текст джерелаThe study of the gut microbiota and especially the effect of its secretory products is an expanding field of research in order to open therapeutic perspectives for nutritional diseases such as obesity or TCA. Among these molecules, the Caseinolytic peptidase B (ClpB) is a bacterial protein having a molecular mimicry in common with α-MSH, a neuropeptide whose anorectic actions are peripheral and central and possible via a microbiota-intestinal-brain communication. Current studies attempt to demonstrate whether this molecular mimicry can confer similar anorectic effects at the ClpB protein. The aim of this thesis was studied the potential pharmacological effects of ClpB protein the regulation of eating behavior. Given that the composition of the gut microbiota is dependent on the food present, the first study was evaluated in vitro the impact of three types of macronutrients on the production and expression of the ClpB protein by E. coli bacteria. Then, it was evaluated whether this protein could influence the secretion of satietogenic peptides like PYY by intestinal enteroendocrine cells using a primary culture of rat intestinal cells. Previous studies of U 1073 laboratory have shown that this protein has been found at the plasma level, the second study was performed in mice submitted to an anorexia model (ABA) to clarify the impact of dietary restriction on the ClpB protein, to better understand its possible involvement in the physiopathology of anorexia nervosa. Finally, the third study was evaluated the pharmacological effects of ClpB protein on food intake in vivo in rodents. The impact of the natural fragmentation of this protein and particularly of one of its fragments on food intake was also evaluated
Magnusson, Jonna. "Lifeloggingkamera som hjälpmedel för familjer där ett barn har autism: En utvärdering av Narrative Clip : En kvalitativ studie hur Narrative Clip kan användas som ett hjälpmedel." Thesis, Linköpings universitet, Institutionen för datavetenskap, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110129.
Повний текст джерелаLiu, Zhonghua. "Characterization of two AAA+ proteins : Escherichia coli ClpB and human torsina /." Search for this dissertation online, 2005. http://wwwlib.umi.com/cr/ksu/main.
Повний текст джерелаRaschperger, Elisabeth. "Studies on CAR and CLMP, two proteins of epithelial tight junctions /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-909-2/.
Повний текст джерелаPolavarapu, Anjaneya Prasad. "Exploring Molecular Interactions : Synthesis and Studies of Clip-Shaped Molecular Hosts." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8220.
Повний текст джерелаLee, Mary Elizabeth Ph D. Massachusetts Institute of Technology. "Regulation of ClpP : role of substrate gating and activation by ClpX." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/58374.
Повний текст джерела"January 2010." Cataloged from PDF version of thesis.
Includes bibliographical references.
AAA+ self-compartmentalized proteases are an important class of proteome regulators that operate to selectively degrade protein substrates. All of these enzymes share the architectural theme of a hexameric ring unfoldase stacked axially onto a barrel-like peptidase, with six- or seven-fold symmetry and sequestered active sites. ClpXP is a model self-compartmentalized protease composed of the regulator ClpX and the serine protease ClpP. Proteolysis occurs by ClpX-dependent substrate selection, unfolding, and translocation into the degradation lumen of ClpP, where rapid and relatively non-specific peptide hydrolysis generates small peptide products. Prior work had shown that ClpP is unable to degrade polypeptides in the absence of ClpX, suggesting the existence of a mechanism that inhibits the activity of free ClpP. Structures of free ClpP show active sites geometrically competent to perform peptide-hydrolysis chemistry. However, some biochemical results suggested that N-terminal ClpP residues, which line the axial entrance pores, allosterically regulate these active sites. Through measurements of ClpP active-site reactivity, degradation of size-varied peptides, and mutagenesis of the N-termini, I found that peptide degradation is inhibited by steric occlusion, maintained by the N-terminal 3-stem loop and a-helix A of ClpP. The N-termini also participate in specifying substrate choice, as mutations within the axial channel prevent degradation of peptides containing stretches of charged amino acids. These data support a model in which ClpX binding opens the axial pore of ClpP to facilitate polypeptide translocation.
(cont.) Additional residues in ClpP that are important for its function were identified by a selection for dominant-negative mutants impaired in ClpXP-dependent proteolysis. Biochemical studies and mapping of these mutations onto the structure of ClpP suggest that these variants are defective in tetradecamer assembly, peptide binding at the active sites, and ClpX binding. This work provides a foundation for further investigations of the mechanisms of ClpP assembly, degradation, and interactions with ClpX.
by Mary Elizabeth Lee.
Ph.D.
Nuez, Catherine. "Méthodes d'analyse en microdialyse cérébrale et sanguine par CLHP-SM-SM." Mulhouse, 1996. http://www.theses.fr/1996MULH0428.
Повний текст джерелаEriksson, Marcus. "A CLP(FD)-based model checker for CTL." Thesis, Linköping University, Department of Computer and Information Science, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-109.
Повний текст джерелаModel checking is a formal verification method where one tries to prove or disprove properties of a formal system. Typical systems one might want to prove properties within are network protocols and digital circuits. Typical properties to check for are safety (nothing bad ever happens) and liveness (something good eventually happens).
This thesis describes an implementation of a sound and complete model checker for Computation Tree Logic (CTL) using Constraint Logic Programming over Finite Domains (CLP(FD)). The implementation described uses tabled resolution to remember earlier computations, is parameterised by choices of computation strategies and can with slight modification support different constraint domains. Soundness under negation is maintained through a restricted form of constructive negation.
The computation process amounts to a fixpoint search, where a fixpoint is reached when no more extension operations has any effect. As results show, the choice of strategies does influence the efficiency of the computation. Soundness and completeness are of course independent of the choice of strategies. Strategies include how to choose the extension operation for the next step and whether to perform global or local rule instantiations, resulting in bottom-up or top-down computations respectively.
Asplund, Mikael. "En optimierande kompilator för SMV till CLP(B)." Thesis, Linköping University, Department of Computer and Information Science, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2805.
Повний текст джерелаThis thesis describes an optimising compiler for translating from SMV to CLP(B). The optimisation is aimed at reducing the number of required variables in order to decrease the size of the resulting BDDs. Also a partitioning of the transition relation is performed. The compiler uses an internal representation of a FSM that is built up from the SMV description. A number of rewrite steps are performed on the problem description such as encoding to a Boolean domain and performing the optimisations.
The variable reduction heuristic is based on finding sub-circuits that are suitable for reduction and a state space search is performed on those groups. An evaluation of the results shows that in some cases the compiler is able to greatly reduce the size of the resulting BDDs.
Strub, Christine Eva. "Untersuchungen zur Struktur und Substratspezifität des AAA+-Chaperons ClpB in Escherichia coli." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979900379.
Повний текст джерелаDany, Hendra. "Application of the ferry clip approach to multi-party and interoperability testing." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/28971.
Повний текст джерелаScience, Faculty of
Computer Science, Department of
Graduate
Lyons, Robert Joseph 1963. "Determining distributed source waveforms in casual, lossy, dispersive, plane-wave (CLDP) materials." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/47722.
Повний текст джерелаIncludes bibliographical references (p. 284-291).
This thesis presents and employs novel mathematics for the inversion of linear, first-kind Fredholm integral equations (IEs) which have a time t dependent response signal, a space z dependent source waveform, and a kernel with time dependence (at each z) corresponding to the impulse response of a thickness z slab of causal, lossy, dispersive, homogeneous material through which planar disturbances propagate according to the wave equation. These materials are called CLDP materials; these IEs are called CLDP IEs. These novel mathematics are applicable to the PESAW (aka PEA) charge recovery method. The proposed inversion method recognizes that the (temporal) Fourier transform of a CLDP IE's response signal can be interpreted as the values of the (spatial) Laplace transform of that IE's source waveform along a Laplace plane path determined by the material's propagation wavenumber k(f). Executing the Laplace transform inversion integral along this CLDP path yields an inverse CLDP IE which recovers the true source waveform provided that source waveform is real, causal, Fourier-transformable, and also satisfies the proposed k(f)-dependent 'CLDP criterion'. The forward and inverse CLDP IEs corresponding to a particular CLDP material model k(f) therefore comprise a particular integral transform relationship applicable to waveforms satisfying the CLDP criterion for that material. The CLDP transform relationship for a lossless/dispersionless material reduces to the (unilateral) Fourier transform. Even without noise, the 'inverse CLDP'-recovered waveform gleaned from an abruptly bandlimited CLDP response signal requires regularization - a generalized Gibbs-Dirichlet kernel dubbed 'the Darrell' comes into effect. The measured (time sampled) PESAW signal is necessarily bandlimited; this thesis investigates regularization via lowpass filtering of the measured signal. Both synthetic and experimental examples are investigated. The focus is on MHz-range signals culled from mm-range polymeric PESAW experiments. A method for determining the requisite model k(f) from measured PESAW signals is also presented and employed.
by R. Joseph Lyons.
Ph.D.
Nolasco, Eduardo Lima. "Estudo da sepse experimental em animais diabéticos e sadios, tratados ou não com insulina." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-03052013-143917/.
Повний текст джерелаSepsis and septic shock are common cause of death in intensive care units (ICU) and according to Brazilian DATA-SUS is one of the major causes of high cost hospitalization. Since diabetic patients have a higher risk of infection and represent approximately 22% of all the septic individuals, it is extremely important to understand the pathophysiology of sepsis in diabetic patients in order to develop interventional measure. The objective of this study is to evaluate the experimental sepsis model by cecal ligation and puncture (CLP 2 punctures) in Wistar healthy males rendered diabetic by intravenous injection of alloxan (42 mg/kg, i.v., 10 days). The study analyzed hematological and biochemical parameters such as urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Furthermore, we studied how insulin treatment could modulate these parameters. After 6 hours of CLP bronchoalveolar (BAL) and peritoneal lavages (PeL) were collected and a cytokine profile was accessed: interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, cytokine-induced neutrophill chemoattractant (CINC)-1, CINC-2. Samples from kidney, lung and liver were collected for morphological analysis and for the measurement of myeloperoxidase activity (MPO). After 6 hours of CLP, red blood cell count, hemoglobin, platelet count, cytokine profile and cellularity of the BAL did not change. In addition, insulin treatment did not change these parameters. CLP caused a decrease in total leukocyte count in both groups diabetic and control rats. Moreover, this model induced a rise in total PeL cellularity with a predominance of polymorfonuclear cells, which was similar in both groups control and diabetic rats. In the PeL, proinflammatory cytokines such as IL-1β, IL-6, CINC-1, CINC-2 and the antinflammatory cytokine IL-10 were enhanced. The TNF-α concentrations remained similar in both groups. Hepatic markers inferring in hepatocyte dysfunction were higher in the diabetes-induced animals what was in part restored after insulin administration. Regarding renal markers, only urea levels were enhanced in diabetic rats and worsened after CLP induction. Morphological changes, such as capillary dilatation and reduced Bowman space was already observed in the kidney of animals with sepsis, insulin treatment did not corrected the values. These data suggest that insulin had a hepato-protective effect but further changes were not observed after insulin treatment.
Salas, Erika. "Les pigments du vin rouge : étude des réactions directes entre anthocyanes et flavanols." Montpellier, ENSA, 2005. http://www.theses.fr/2005ENSA0009.
Повний текст джерелаAtanasova, Vessela Dimitrova. "Réactions des composés phénoliques dans les vins rouges induites par la technique de micro-oxygénation : caractérisation de nouveaux produits de condensation des anthocyanes avec l'acétaldéhyde." Montpellier, ENSA, 2003. http://www.theses.fr/2003ENSA0002.
Повний текст джерелаIn this study, the influence of micro-oxygenation on the phenolic composition and the colour characteristics of a wine were studied. Significant changes between the oxygenated and control wines were observed after seven months of storage. Principal Component Analysis applied to the wines at various stages of ageing showed c1early that the wines are separated, along the first axis, according to their storage time, and along the second axis, according to oxygenation. Over time, the concentration of the native anthocyanins gradually decreased and more stable structures such as pyranoanthocyanins and T-A+ pigments accumulated. Micro-oxygenation favoured the mechanisms involving acetaldehyde, i. E. Condensation reactions between phenolic compounds and acetaldehyde, and reactions of cyc1oaddition between anthocyanins, flavanols and acetaldehyde. During the study of these mechanisms in mode! solutions, two new structures were detected, resulting either from the direct reaction between anthocyanins (Mv 3g-Mv 3g), or induced by acetaldehyde (Mv 3g-ethyl-Mv 3g). The last derivative was also detected in the wines. Taking into account the interest that this molecule presents in the field of enology, it was purified in order to investigate its structure by NMR and to study its physical-chemical properties. An oligomeric fraction characterised by DPm 3,1 was also isolated. The detection of ethyl-linked Mv 3g oligomers indicates that the C6 position of the anthocyanins is also reactive
Jesus, Aline Alves de. "Hidrogênio molecular inibe a resposta inflamatória e previne o dano cognitivo em ratos submetidos ao choque séptico." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17134/tde-07022019-132708/.
Повний текст джерелаThe central nervous system is one of the first regions to be affected during Sepsis and septic shock, which contributes to the increased rate of morbidity and mortality. Patients with severe sepsis may present acute neuronal dysfunction such as delirium, disorientation, and unconscious. In the long term, cognitive damage can occur causing the commitment of learning and memory formation. Studies show that during the exacerbated systemic inflammatory response, inflammatory mediators present in the systemic circulation, are able to reach the CNS and cause the activation of glial cells, leading to a state of neuroinflammation. In this process, some CNS structures such as the hippocampus are more vulnerable to the action of reactive oxygen species (ROS), and to inflammatory mediators produced excessively during sepsis. In this context, the investigation of new therapeutic strategies that are capable of attenuating the exacerbated inflammatory response is necessary. Thus, the present project aimed to investigate the probable antioxidant and anti-inflammatory properties of molecular hydrogen (H2), as well as its possible neuroprotective action in rats submitted to polymicrobial sepsis induced by ligature and cecal puncture (CLP). In order to check this hypothesis, the project was divided into two experimental protocols. In the first protocol Wistar rats submitted to CLP or Sham surgery were submitted to 2% H2 inhalation treatment for a period of 1h for 10 consecutive days, and soon after they underwent behavioral tests to evaluate habituation memory, discriminative and aversive. In the second protocol the animals were treated with H2 inhalation for a period of 3h and 24h, and at the end of the treatment, they were decapitated for blood and brain collection. Plasma was already used for nitrate dosage, lipid peroxidation, antioxidant enzymes and inflammatory cytokines. From the results of the behavioral tests, we observed that treatment with H2 inhalation during the experimental sepsis prevented memory loss and cognitive damage, and was able to decrease the levels of acute-phase inflammatory cytokines such as IL-1?, IL -6 and TNF? in the prefrontal cortex and hippocampus. The therapeutic strategy was also able to decrease plasma TBARS levels. We also observed an increase in the concentration of the enzyme catalase in H2-treated animals. Together the results indicate that molecular hydrogen was able to inhibit the inflammatory response and prevent cognitive damage, acting as a neuroprotective substance, in rats submitted to experimental septic shock
Cordelières, Fabrice. "Quelle fonction pour la CLIP-170 ? Recherche de partenaires et nouveaux outils d'investigation." Phd thesis, Université Paris Sud - Paris XI, 2003. http://tel.archives-ouvertes.fr/tel-00432222.
Повний текст джерелаGuo, Dong. "Analytical investigation of block shear of coped beams with welded clip angles connection." Thesis, University of Macau, 2007. http://umaclib3.umac.mo/record=b1636330.
Повний текст джерелаLe, Solleu Hervé. "Le bromazépam : de l'anxiolyse à la toxicité : dosage dans le plasma par CLHP." Bordeaux 2, 1992. http://www.theses.fr/1992BOR2P031.
Повний текст джерела