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Готові списки джерел за темами / Chlamydomonas reinhardtii mutant

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Добірка наукової літератури з теми "Chlamydomonas reinhardtii mutant"

Автор: Grafiati

Опубліковано: 11 березня 2023

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Статті в журналах з теми "Chlamydomonas reinhardtii mutant"

1

Llamas, Ángel, Manuel Tejada-Jimenez, David González-Ballester, José Javier Higuera, Guenter Schwarz, Aurora Galván, and Emilio Fernández. "Chlamydomonas reinhardtii CNX1E Reconstitutes Molybdenum Cofactor Biosynthesis in Escherichia coli Mutants." Eukaryotic Cell 6, no. 6 (April 6, 2007): 1063–67. http://dx.doi.org/10.1128/ec.00072-07.

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ABSTRACT We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G and CNX1E, and expressed them and their chimeric fusions in Chlamydomonas and Escherichia coli. In all cases, the wild-type phenotype was restored in individual mutants as well as in a CNX1G CNX1E double mutant. Therefore, CrCNX1E is the first eukaryotic protein able to complement an E. coli moeA mutant.

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2

Posewitz, M. C., P. W. King, S. L. Smolinski, R. Davis Smith, A. R. Ginley, M. L. Ghirardi, and M. Seibert. "Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii." Biochemical Society Transactions 33, no. 1 (February 1, 2005): 102–4. http://dx.doi.org/10.1042/bst0330102.

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The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H2 production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective in H2 production. Two mutants of particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble an active [FeFe]-hydrogenase. This is the first reported C. reinhardtii mutant that is not capable of producing any H2. The second mutant, sta7-10, is not able to accumulate insoluble starch and has significantly lowered H2-photoproduction rates in comparison with the wild-type. In hydEF-1, anaerobiosis induces transcription of the two reported C. reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated.

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3

Suzuki, Kensaku, Laura Fredrick Marek, and Martin H. Spalding. "A Photorespiratory Mutant of Chlamydomonas reinhardtii." Plant Physiology 93, no. 1 (May 1, 1990): 231–37. http://dx.doi.org/10.1104/pp.93.1.231.

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4

Remacle, C., F. Duby, P. Cardol, and R. F. Matagne. "Mutations inactivating mitochondrial genes in Chlamydomonas reinhardtii." Biochemical Society Transactions 29, no. 4 (August 1, 2001): 442–46. http://dx.doi.org/10.1042/bst0290442.

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Chlamydomonas reinhardtii is now becoming a useful model for the study of mitochondrial genetics in a photosynthetic organism. The small (15.8 kb) mitochondrial genome C. reinhardtii has been sequenced completely and all the genes have been identified. Several mutants inactivated in mitochondrial genes encoding components of the respiratory complexes I, III and IV have been characterized at the molecular level. Assembly of complex I in several mutant strains and mapping of mitochondrial mutations by recombinational analysis are also described.

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5

Kuchka, Michael R., and Jonathan W. Jarvik. "Short-Flagella Mutants of Chlamydomonas reinhardtii." Genetics 115, no. 4 (April 1, 1987): 685–91. http://dx.doi.org/10.1093/genetics/115.4.685.

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ABSTRACT Six short-flagella mutants were isolated by screening clones of mutagenized Chlamydomonas for slow swimmers. The six mutants identify three unlinked Mendelian genes, with three mutations in gene shf-1, two in shf-2 and one in shf-3. shf-1 and shf-2 have been mapped to chromosomes VI and I, respectively. Two of the shf-1 mutations have temperature-sensitive flagellar-assembly phenotypes, and one shf-2 mutant has a cold-sensitive phenotype. shf shf double mutants were constructed; depending on the alleles present they showed either flagellaless or short-flagella phenotypes. Phenotypic revertants of shf-1 and shf-2 mutants were isolated, and certain of them were found to carry extragenic suppressors, some dominant and some recessive. We suspect that the shf mutations affect components of a specific flagellar size-control system, the existence of which has been suggested by a variety of physiological experiments.

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6

Tam, L. W., and P. A. Lefebvre. "Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis." Genetics 135, no. 2 (October 1, 1993): 375–84. http://dx.doi.org/10.1093/genetics/135.2.375.

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Abstract Chlamydomonas is a popular genetic model system for studying many cellular processes. In this report, we describe a new approach to isolate Chlamydomonas genes using the cloned nitrate reductase gene (NIT1) as an insertional mutagen. A linearized plasmid containing the NIT1 gene was introduced into nit1 mutant cells by glass-bead transformation. Of 3000 Nit+ transformants examined, 74 showed motility defects of a wide range of phenotypes, suggesting that DNA transformation is an effective method for mutagenizing cells. For 13 of 15 such motility mutants backcrossed to nit- mutant strains, the motility phenotype cosegregated with the Nit+ phenotype, indicating that the motility defects of these 13 mutants may be caused by integration of the plasmid. Further genetic analysis indicated that three of these mutants contained alleles of previously identified loci: mbo2 (move backward only), pf13 (paralyzed flagella) and vfl1 (variable flagellar number). Three other abnormal-flagellar-number mutants did not map to any previously described loci at which mutations produce similar phenotypes. Genomic sequences flanking the integrated plasmid in the mbo2 and vfl1 mutants were isolated and used as probes to obtain wild-type genomic clones, which complemented the motility defects upon transformation into cells. Our results demonstrate the potential of this new approach for cloning genes identified by mutation in Chlamydomonas.

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7

Dutcher, S. K., R. E. Galloway, W. R. Barclay, and G. Poortinga. "Tryptophan analog resistance mutations in Chlamydomonas reinhardtii." Genetics 131, no. 3 (July 1, 1992): 593–607. http://dx.doi.org/10.1093/genetics/131.3.593.

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Abstract Forty single gene mutations in Chlamydomonas reinhardtii were isolated based on resistance to the compound 5'-methyl anthranilic acid (5-MAA). In other organisms, 5-MAA is converted to 5'-methyltryptophan (5-MT) and 5-MT is a potent inhibitor of anthranilate synthase, which catalyzes the first committed step in tryptophan biosynthesis. The mutant strains fall into two phenotypic classes based on the rate of cell division in the absence of 5-MAA. Strains with class I mutations divide more slowly than wild-type cells. These 17 mutations map to seven loci, which are designated MAA1 to MAA7. Strains with class II mutations have generation times indistinguishable from wild-type cells, and 7 of these 23 mutations map to loci defined by class I mutations. The remainder of the class II mutations map to 9 other loci, which are designated MAA8-MAA16. The maa5-1 mutant strain excretes high levels of anthranilate and phenylalanine into the medium. In this strain, four enzymatic activities in the tryptophan biosynthetic pathway are increased at least twofold. These include the combined activities of anthranilate phosphoribosyl transferase, phosphoribosyl anthranilate isomerase, indoleglycerol phosphate synthetase and anthranilate synthase. The slow growth phenotypes of strains with class I mutations are not rescued by the addition of tryptophan, but the slow growth phenotype of the maa6-1 mutant strain is partially rescued by the addition of indole. The maa6-1 mutant strain excretes a fluorescent compound into the medium, and cell extracts have no combined anthranilate phosphoribosyl transferase, phosphoribosyl anthranilate isomerase and indoleglycerol phosphate synthetase activity. The MAA6 locus is likely to encode a tryptophan biosynthetic enzyme. None of the other class I mutations affected these enzyme activities. Based on the phenotypes of double mutant strains, epistatic relationships among the class I mutations have been determined.

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8

Lin, Huawen, Zhengyan Zhang, Carlo Iomini, and Susan K. Dutcher. "Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii." Open Biology 8, no. 3 (March 2018): 170211. http://dx.doi.org/10.1098/rsob.170211.

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Intraflagellar transport moves proteins in and out of flagella/cilia and it is essential for the assembly of these organelles. Using whole-genome sequencing, we identified splice site mutations in two IFT genes, IFT81 ( fla9 ) and IFT121 ( ift121-2 ), which lead to flagellar assembly defects in the unicellular green alga Chlamydomonas reinhardtii . The splicing defects in these ift mutants are partially corrected by mutations in two conserved spliceosome proteins, DGR14 and FRA10. We identified a dgr14 deletion mutant, which suppresses the 3′ splice site mutation in IFT81 , and a frameshift mutant of FRA10 , which suppresses the 5′ splice site mutation in IFT121 . Surprisingly, we found dgr14-1 and fra10 mutations suppress both splice site mutations. We suggest these two proteins are involved in facilitating splice site recognition/interaction; in their absence some splice site mutations are tolerated. Nonsense mutations in SMG1 , which is involved in nonsense-mediated decay, lead to accumulation of aberrant transcripts and partial restoration of flagellar assembly in the ift mutants. The high density of introns and the conservation of noncore splicing factors, together with the ease of scoring the ift mutant phenotype, make Chlamydomonas an attractive organism to identify new proteins involved in splicing through suppressor screening.

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9

Spalding, Martin H., Kyujung Van, Yingjun Wang, and Yoshiko Nakamura. "Acclimation of Chlamydomonas to changing carbon availability." Functional Plant Biology 29, no. 3 (2002): 221. http://dx.doi.org/10.1071/pp01182.

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Aquatic organisms, including Chlamydomonas reinhardtii, are faced with a variable supply of dissolved inorganic carbon (Ci). Accordingly, C. reinhardtii has the ability to acclimate to the changing Ci supply through a variety of responses, including induction of a CO2 concentrating mechanism (CCM) when Ci is limiting. The CCM uses active Ci uptake to accumulate a high internal concentration of bicarbonate, which is dehydrated by a specific thylakoid carbonic anhydrase to supply CO2, the substrate used in photosynthesis. In addition to the changes demonstrably related to the function of the CCM, C. reinhardtii exhibits several other acclimation responses to limiting Ci, such as changes in cellular organization and induction or upregulation of several genes. A key area currently under investigation is how C. reinhardtii cells recognize the change in Ci or CO2 concentration, and transduce that signal into needed gene expression changes. Mutational analyses are proving very useful for learning more about the CCM and about the acclimation response to changes in Ci availability. Cloning of the gene disrupted in cia5, a mutant apparently unable to acclimate to limiting Ci, has opened opportunities for more rapid progress in understanding the signal transduction pathway. The Cia5 gene appears to encode a transcription factor that may control, either directly or indirectly, much of the gene expression responses to limiting Ci in C. reinhardtii. Several additional new mutants with potential defects in the signal transduction pathway have been isolated, including three new alleles of cia5.

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Unal, Dilek, and Fazilet Ozlem Cekic. "Cold acclimation of SnRK2.2 kinases mutant Chlamydomonas reinhardtii." Phycological Research 67, no. 3 (March 19, 2019): 202–7. http://dx.doi.org/10.1111/pre.12371.

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Дисертації з теми "Chlamydomonas reinhardtii mutant"

1

Johnston, Heather Grunkemeyer. "Time-resolved fluorescence studies of wild type and mutant photosystem II reaction centers isolated from Chlamydomonas reinhardtii /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488202171194972.

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2

Lown, Felicity Jane. "Respiratory mutants of chlamydomonas." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247.

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3

Patel, Vaishali. "Analysis of photosystem 1 mutants in Chlamydomonas reinhardtii." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266592.

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4

Huang, Mingya. "Secondary Level Screening of Chlamydomonas Reinhardtii Mutants Defective in Circadian Gene Expression." TopSCHOLAR®, 2001. http://digitalcommons.wku.edu/theses/667.

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To elucidate the signal transduction chain mediating circadian clock control, this work focuses on the isolation of Chlamydomonas reinhardtii mutants which are defective in circadian gene expression. In a previous study, the reporter gene ARS2 encoding the arylsulfatase enzyme was fused to the promoter of the circadian-regulated CABII-1 gene and transformed into the Chlamydomonas nucleus. The ble marker was introduced into the genome of this transformant via insertional mutagenesis to generate mutants defective in circadian CABII-1 expression. Potential mutants were selected based on aberrant single-point accumulative arylsulfatase activity. In this study, the arylsulfatase activity over the entire growth cycle was further investigated in these mutants and the reliability of the single-point screen was assessed. Of the 16 strains whose accumulative arylsulfatase activity did not differ from the nonmutagenized control in the single-point screen, 12 still showed no significant difference in a multiple-point screen. Of the 9 potential mutants with significant difference to the control in the single-point screen, 3 showed no significant difference in the multiple-point screen. Subsequently, 8 of the candidate mutants with aberrant reporter enzyme activity in the multiple-point screen were characterized by the abundance of their mRNA. The peak-to-trough ratio of CABII-1 and ARS2 transcript abundance was significantly reduced in 4 of these mutants.

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5

WANDOLOSKI, MELISSA ANN. "ANALYSIS OF THE EYE2 PROTEIN IN EYESPOT ASSEMBLY MUTANTS OF CHLAMYDOMONAS REINHARDTII." Thesis, The University of Arizona, 2008. http://hdl.handle.net/10150/192252.

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Torres, Romero Ismael. "Dynamics of lipid reserves in the model microalga Chlamydomonas reinhardtii." Thesis, Aix-Marseille, 2020. http://www.theses.fr/2020AIXM0023.

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D'importants efforts de recherche ont été déployés pour domestiquer les microalgues afin de produire des biocarburants durables et d'autres composés à haute valeur ajoutée. Les conditions utilisées pour enrichir la biomasse de microalgues avec des réserves de carbone, triacylglycérols (TAG ou huiles) et l'amidon, cependant, nuisent gravement à la croissance cellulaire et compromettent donc la productivité. Le but de cette thèse est d’analyser le lien entre la division cellulaire et le stockage du carbone, ainsi que de comprendre la biogénèse des gouttelettes de lipides (LDs), le principal site subcellulaire de stockage des TAGs.Ainsi, nous avons d'abord étudié l'incompatibilité entre le stockage du carbone et la croissance cellulaire en caractérisant génétiquement et biochimiquement des mutants de Chlamydomonas reinhardtii dépourvus de protéine CDC5. Nous démontrons son implication dans le cycle cellulaire et montrons qu'un ralentissement de la division cellulaire entraîne un flux d'énergie et de carbone vers la synthèse des TAGs et de l'amidon sans arrêter la croissance cellulaire. Deuxièmement, nous avons identifié et caractérisé une α/β hydrolase putative (CrABHD1), l'une des principales protéines associées aux LDs chez Chlamydomonas. La protéine recombinante CrABHD1 purifiée chez Escherichia coli hydrolyse du lyso-DGTS pour produire un acide gras libre et une glycérol-N,N,N-triméthylhomosérine (GTH). Nous avons découvert une nouvelle protéine associée à la LD et démontré sa capacité à augmenter la teneur en lipides des microalgues, ce qui devrait avoir des implications importantes pour une bioéconomie plus verte
Large research efforts have been put to domesticate microalgae for production of sustainable biofuels and other valuable compounds. Triacylglycerols (TAGs, or oils) and starch are the major forms of carbon storage in green algal cells. However, the conditions used to enrich microalgal biomass with these carbon reserves severely undermine cell growth therefore compromising productivity. An economically viable production of lipids from microalgae requires a deeper and integrated understanding of lipid synthesis, storage and cell division. The goal of this thesis is to dissect the connection between cell division and carbon storage, and to understand the biogenesis of the lipid droplet (LD), the major subcellular site where TAGs are stored. Toward this goal, we first investigated the incompatibility between carbon storage and cell growth. By characterizing genetically and biochemically mutants of Chlamydomonas reinhardtii deficient in CDC5 protein, we demonstrate its implication in the cell cycle and show that a slowdown in cell division entails a diverted flow of energy and carbon towards the synthesis of TAGs and starch without arresting cell growth. Secondly, we identified and characterized a putative α/β-fold hydrolase (CrABHD1), one of the major proteins associated to LDs in Chlamydomonas. The CrABHD1 recombinant protein purified from Escherichia coli hydrolyzes lyso-DGTS to produce a free fatty acid and a glycerol-N,N,N-trimethylhomoserine (GTH). We have discovered a novel LD-associated protein and demonstrated its capacity in increasing lipid content in microalgae, which should have important implications for a greener bioeconomy

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Lucas, Pierre-Louis. "Etude et ingénierie de la N-glycosylation des protéines chez la microalgue verte chlamydomanas reinhardtii." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR061/document.

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Actuellement, plus de 70% des biomédicaments commercialisés sont des glycoprotéines recombinantes. Les coûts élevés de production de ces biomédicaments ont poussé les scientifiques à développer des organismes de production alternatifs. Récemment, les microalgues ont été proposées en tant que potentiel système de production compte-tenu de leur rapidité de croissance et de leurs faibles coûts de production. Cependant, avant de produire des biomédicaments industriels chez les microalgues, il est impératif de s’assurer que les modifications post-traductionnelles, comme la N-glycosylation, soit conservées et compatibles avec une utilisation thérapeutique. Dans ce contexte, l’étude de la Nglycosylation de deux microalgues modèles, Chlamydomonas reinhardtii (microalgue verte) et Phaeodactylum tricornutum (diatomée) a été réalisée. Dans un premier temps, l’ingénierie de la N-glycosylation de C. reinhardtii a été initiée en exprimant une Nacétylglucosaminyltransférase I (GnT I) hétérologue. Les résultats obtenus ont permis de réévaluer les voies de N-glycosylation de C. reinhardtii et de montrer que cette microalgue synthétise une structure glycannique linéaire qui n’est pas substrat de la GnT I. Dans un second temps, un protocole d’extraction et de caractérisation des précurseurs glycanniques de C. reinhardtii et P. tricornutum a été développé et appliqué pour déterminer la structure des précurseurs glycanniques dans ces espèces. Enfin, la caractérisation de deuxxylosyltransférases potentielles (XTA et XTB) de C. reinhardtii a été menée en utilisant des mutants d’insertion et des analyses des N-glycannes par spectrométrie de masse. Cette étude a confirmé les rôles spécifiques de XTA et XTB dans la voie de N-glycosylation de C. reinhardtii
Currently, more than 70% of the commercialized biopharmaceuticals are glycoproteins. The high production costs lead scientists to develop alternative organisms suitable for such production. Recently, microalgae emerged as a potential interesting production system thanks to their quick growth rate and low production costs. However, prior to start industrial glycoproteins production in microalgae, protein post-translational modifications like Nglycosylation, must be carefully controlled. This PhD thesis focused on the analysis of the Nglycosylation pathway of two different microalgae, Chlamydomonas reinhardtii (greenmicroalgae) and Phaeodactylum tricornutum (diatom). In order to start N-glycan engineering, heterologous N-acetylglucosaminyltransferase I (GnT I) sequences were expressed in C.reinhardtii. This study demonstrated that C. reinhardtii synthetize a linear N-glycan unsuitable for GnT I activity and allows the reinvestigation of the C. reinhardtii N-glycosylation pathway. A second chapter of this work focus on the optimization of a protocol suitable for analyzing the structure of the Dolichol N-linked precursors of C. reinhardtii and P. tricornutum. Lastly, two potential xylosyltransferases (XTA and XTB) from C. reinhardtii were characterized using insertional mutants and N-glycomic analyses by mass spectrometry approaches. This work allows us to propose specific involvement of XTA and XTB in the xylosylation processing of C.reinhardtii N-glycans

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8

Yuan, Wei. "Screening for Mutants in the Output Pathway of the Circadian Clock in Chlamydomonas Reinhardtii." TopSCHOLAR®, 1999. http://digitalcommons.wku.edu/theses/764.

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The circadian clock is a basic component of biological systems and has been found in many organisms. It is composed by three major components: the input pathway, the oscillator and the output pathway. The purpose of this research is to study the output pathway of the circadian clock in Chlamydomonas reinhardtii strain Carnil by screening mutants, which were generated by insertional mutagenesis via glass bead transformation. The plasmid pSP124S containing the ble marker was used to introduce mutations. The CABII-1 gene has been reported to show a circadian rhythm in expression. The reporter gene ARS2 that was transcriptionally fused to the promoter of the CABII-1 gene was then analyzed. The screening process was optimized to be less time-consuming. Eighteen mutants with significantly low ARS2 accumulated expressions were found from 1004 transformants, which implies that there may be an activator involved in the circadian phenotype of CABII-1.

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9

Wang, Fei. "Molecular and functional analysis of photosynthesis-related mutants from Chlamydomonas reinhardtii and Arabidopsis thaliana." Diss., Ludwig-Maximilians-Universität München, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-173295.

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10

Castonguay, Andrew David. "Analysis of mutants impaired for respiratory growth in the model photosynthetic alga, Chlamydomonas reinhardtii." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1619140884575211.

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Частини книг з теми "Chlamydomonas reinhardtii mutant"

1

Suzuki, Kensaku, Laura Fredrick Marek, and Martin H. Spalding. "A Phosphoglycolate Phosphatase Mutant of Chlamydomonas reinhardtii." In Current Research in Photosynthesis, 3303–6. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0511-5_744.

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2

Avilan, L., B. Gontero, and J. Ricard. "Isolation of Different Forms of Phosphoribulokinase from Mutant Chlamydomonas Reinhardtii Cells." In Photosynthesis: from Light to Biosphere, 4091–94. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_962.

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3

Merchant, Sabeeha, Lawrence Bogorad, Sheila A. Iverson, and John H. Richards. "Molecular Characterization of Chlamydomonas Reinhardtii, ac-208: A Plastocyanin-Less Mutant." In Progress in Photosynthesis Research, 663–66. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0519-6_137.

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4

Andronis, C., J. R. Durrant, B. A. Diner, S. Merry, D. R. Klug, and P. J. Nixon. "Construction and Initial Characterisation of a D2-LEU205TYR Mutant of Chlamydomonas Reinhardtii." In Photosynthesis: from Light to Biosphere, 839–42. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_200.

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5

Xiong, Jin, Ronald Hutchison, Richard Sayre, and Govindjee. "Characterization of a Site-Directed Mutant (D1-Arginine 269-Glycine) of Chlamydomonas reinhardtii." In Photosynthesis: from Light to Biosphere, 575–78. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_134.

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6

Ruffle, S. V., H. O’Connor, A. J. Cheater, S. Purton, and J. H. A. Nugent. "The construction and analysis of a disruption mutant of psbH in Chlamydomonas reinhardtii." In Photosynthesis: from Light to Biosphere, 2603–6. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_613.

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7

Minagawa, Jun, David M. Kramer, Atsuko Kanazawa, and Antony R. Crofts. "Aberrant Electron Transfer in D1-Y161F Mutant from Chlamydomonas Reinhardtii — A Donor-Side Photoinhibition." In Photosynthesis: from Light to Biosphere, 3445–48. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_811.

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8

Husic, Diane W., and N. E. Tolbert. "Inhibition of Glycolate and D-Lactate Metabolism in a Mutant of Chlamydomonas Reinhardtii Deficient in Mitochondrial Respiration." In Progress in Photosynthesis Research, 557–60. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0516-5_117.

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9

Ma, Yunbing, Megan M. Hartman, and James V. Moroney. "Transcriptional Analysis of the Three Phosphoglycolate Phosphatase Genes in Wild Type and the pgp1 Mutant of Chlamydomonas Reinhardtii." In Advanced Topics in Science and Technology in China, 315–18. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-32034-7_66.

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10

Shibata, Hideyuki, Masako Yamamoto, Ryo Sato, Elizabeth H. Harris, Nicholas W. Gillham, and John E. Boynton. "Isolation and Characterization of a Chlamydomonas Reinhardtii Mutant Resistant to an Experimental Herbicide S-23142, Which Inhibits Chlorophyll Synthesis." In Research in Photosynthesis, 567–70. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-009-0383-8_124.

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Тези доповідей конференцій з теми "Chlamydomonas reinhardtii mutant"

1

Sethi, Purnima, Mohit Prasad, and Sukhdev Roy. "All-optical switching in LOV2-C250S protein mutant from Chlamydomonas reinhardtii green algae." In 2009 International Conference on Emerging Trends in Electronic and Photonic Devices & Systems (ELECTRO-2009). IEEE, 2009. http://dx.doi.org/10.1109/electro.2009.5441039.

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2

Puzansky, R. K., and M. F. Shishova. "Metabolomic and molecular genetic aspects of trophic adaptation of mutants Chlamydomonas reinhardtii." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-366.

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3

Chekunova, E. M., A. B. Matiiv, and T. S. Ushkina. "INVESTIGATIONS OF THE GENETIC MECHANISMS OF PLANT CELL ADAPTATION TO THE LIGHT ON THE MODEL OF CHLOROPHYLL-LESS MUTANTS OF UNICELLULAR GREEN ALGAE CHLAMYDOMONAS REINHARDTII." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1003-1007.

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Звіти організацій з теми "Chlamydomonas reinhardtii mutant"

1

Ohad, Itzhak, and Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613031.bard.

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The aim of this research project was to obtain information on the role of the cytochrome b559 in the function of Photosystem-II (PSII) with special emphasis on the light induced photo inactivation of PSII and turnover of the photochemical reaction center II protein subunit RCII-D1. The major goals of this project were: 1) Isolation and sequencing of the Chlamydomonas chloroplast psbE and psbF genes encoding the cytochrome b559 a and b subunits respectively; 2) Generation of site directed mutants and testing the effect of such mutation on the function of PSII under various light conditions; 3) To obtain further information on the mechanism of the light induced degradation and replacement of the PSII core proteins. This information shall serve as a basis for the understanding of the role of the cytochrome b559 in the process of photoinhibition and recovery of photosynthetic activity as well as during low light induced turnover of the D1 protein. Unlike in other organisms in which the psbE and psbF genes encoding the a and b subunits of cytochrome b559, are part of an operon which also includes the psbL and psbJ genes, in Chlamydomonas these genes are transcribed from different regions of the chloroplast chromosome. The charge distribution of the derived amino-acid sequences of psbE and psbF gene products differs from that of the corresponding genes in other organisms as far as the rule of "positive charge in" is concerned relative to the process of the polypeptide insertion in the thylakoid membrane. However, the sum of the charges of both subunits corresponds to the above rule possibly indicating co-insertion of both subunits in the process of cytochrome b559 assembly. A plasmid designed for the introduction of site-specific mutations into the psbF gene of C. reinhardtii. was constructed. The vector consists of a DNA fragment from the chromosome of C. reinhardtii which spans the region of the psbF gene, upstream of which the spectinomycin-resistance-conferring aadA cassette was inserted. This vector was successfully used to transform wild type C. reinhardtii cells. The spectinomycin resistant strain thus obtained can grow autotrophically and does not show significant changes as compared to the wild-type strain in PSII activity. The following mutations have been introduced in the psbF gene: H23M; H23Y; W19L and W19. The replacement of H23 involved in the heme binding to M and Y was meant to permit heme binding but eventually alter some or all of the electron transport properties of the mutated cytochrome. Tryptophane W19, a strictly conserved residue, is proximal to the heme and may interact with the tetrapyrole ring. Therefore its replacement may effect the heme properties. A change to tyrosine may have a lesser affect on the potential or electron transfer rate while a replacement of W19 by leucine is meant to introduce a more prominent disturbance in these parameters. Two of the mutants, FW19L and FH23M have segregated already and are homoplasmic. The rest are still grown under selection conditions until complete segregation will be obtained. All mutants contain assembled and functional PSII exhibiting an increased sensitivity of PSII to the light. Work is still in progress for the detailed characterization of the mutants PSII properties. A tobacco mutant, S6, obtained by Maliga and coworkers harboring the F26S mutation in the b subunit was made available to us and was characterized. Measurements of PSII charge separation and recombination, polypeptide content and electron flow indicates that this mutation indeed results in light sensitivity. Presently further work is in progress in the detailed characterization of the properties of all the above mutants. Information was obtained demonstrating that photoinactivation of PSII in vivo initiates a series of progressive changes in the properties of RCII which result in an irreversible modification of the RCII-D1 protein leading to its degradation and replacement. The cleavage process of the modified RCII-D1 protein is regulated by the occupancy of the QB site of RCII by plastoquinone. Newly synthesized D1 protein is not accumulated in a stable form unless integrated in reassembled RCII. Thus the degradation of the irreversibly modified RCII-D1 protein is essential for the recovery process. The light induced degradation of the RCII-D1 protein is rapid in mutants lacking the pD1 processing protease such as in the LF-1 mutant of the unicellular alga Scenedesmus obliquus. In this case the Mn binding site of PSII is abolished, the water oxidation process is inhibited and harmful cation radicals are formed following light induced electron flow in PSII. In such mutants photo-inactivation of PSII is rapid, it is not protected by ligands binding at the QB site and the degradation of the inactivated RCII-D1 occurs rapidly also in the dark. Furthermore the degraded D1 protein can be replaced in the dark in absence of light driven redox controlled reactions. The replacement of the RCII-D1 protein involves the de novo synthesis of the precursor protein, pD1, and its processing at the C-terminus end by an unknown processing protease. In the frame of this work, a gene previously isolated and sequenced by Dr. Pakrasi's group has been identified as encoding the RCII-pD1 C-terminus processing protease in the cyanobacterium Synechocystis sp. PCC 6803. The deduced sequence of the ctpA protein shows significant similarity to the bovine, human and insect interphotoreceptor retinoid-binding proteins. Results obtained using C. reinhardtii cells exposes to low light or series of single turnover light flashes have been also obtained indicating that the process of RCII-D1 protein turnover under non-photoinactivating conditions (low light) may be related to charge recombination in RCII due to back electron flow from the semiquinone QB- to the oxidised S2,3 states of the Mn cluster involved in the water oxidation process.

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2

Schuster, Gadi, and David Stern. Integrated Studies of Chloroplast Ribonucleases. United States Department of Agriculture, September 2011. http://dx.doi.org/10.32747/2011.7697125.bard.

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Gene regulation at the RNA level encompasses multiple mechanisms in prokaryotes and eukaryotes, including splicing, editing, endo- and exonucleolytic cleavage, and various phenomena related to small or interfering RNAs. Ribonucleases are key players in nearly all of these post-transcriptional mechanisms, as the catalytic agents. This proposal continued BARD-funded research into ribonuclease activities in the chloroplast, where RNase mutation or deficiency can cause metabolic defects and is often associated with plant chlorosis, embryo or seedling lethality, and/or failure to tolerate nutrient stress. The first objective of this proposal was to examined a series of point mutations in the PNPase enzyme of Arabidopsis both in vivo and in vitro. This goal is related to structure-function analysis of an enzyme whose importance in many cellular processes in prokaryotes and eukaryotes has only begun to be uncovered. PNPase substrates are mostly generated by endonucleolytic cleavages for which the catalytic enzymes remain poorly described. The second objective of the proposal was to examine two candidate enzymes, RNase E and RNase J. RNase E is well-described in bacteria but its function in plants was still unknown. We hypothesized it catalyzes endonucleolytic cleavages in both RNA maturation and decay. RNase J was recently discovered in bacteria but like RNase E, its function in plants had yet to be explored. The results of this work are described in the scientific manuscripts attached to this report. We have completed the first objective of characterizing in detail TILLING mutants of PNPase Arabidopsis plants and in parallel introducing the same amino acids changes in the protein and characterize the properties of the modified proteins in vitro. This study defined the roles for both RNase PH core domains in polyadenylation, RNA 3’-end maturation and intron degradation. The results are described in the collaborative scientific manuscript (Germain et al 2011). The second part of the project aimed at the characterization of the two endoribonucleases, RNase E and RNase J, also in this case, in vivo and in vitro. Our results described the limited role of RNase E as compared to the pronounced one of RNase J in the elimination of antisense transcripts in the chloroplast (Schein et al 2008; Sharwood et al 2011). In addition, we characterized polyadenylation in the chloroplast of the green alga Chlamydomonas reinhardtii, and in Arabidopsis (Zimmer et al 2009). Our long term collaboration enabling in vivo and in vitro analysis, capturing the expertise of the two collaborating laboratories, has resulted in a biologically significant correlation of biochemical and in planta results for conserved and indispensable ribonucleases. These new insights into chloroplast gene regulation will ultimately support plant improvement for agriculture.

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