Дисертації з теми "Ceramidase"
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Bayerle, Aline [Verfasser]. "Physiologische Konsequenzen eines gestörten Ceramid Stoffwechsels unter Beteiligung von Ceramidsynthase 3 und saurer Ceramidase = Physiological consequences of a disturbed ceramide metabolism due to altered activities of ceramide synthase 3 and acidic ceramidase / Aline Bayerle." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2015. http://d-nb.info/1225685362/34.
Повний текст джерелаGoltz, Gerit. "Charakterisierung von Ceramidase-Inhibitoren an der humanen Keratinozyten-Zellinie HaCaT." [S.l.] : [s.n.], 2002. http://www.diss.fu-berlin.de/2002/180/index.html.
Повний текст джерелаLucki, Natasha Chrystman. "Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/42804.
Повний текст джерелаLi, Guangbi. "Podocyte Dedifferentiation and Glomerular Injury Mediated by Lysosome Dysfunction: Role of Acid Ceramidase." VCU Scholars Compass, 2017. https://scholarscompass.vcu.edu/etd/5169.
Повний текст джерелаCamacho, Castillo Luz del Carmen. "Acid ceramidase and sphingosine-1-phosphate lyase as biomarkers and therapeutic targets in cancer. (Ceramidasa ácida y Esfingosina-1-fosfato Liasa como biomarcadores y dianas terapéuticas en cáncer)." Doctoral thesis, Universitat de Barcelona, 2011. http://hdl.handle.net/10803/21624.
Повний текст джерелаMarkus, Carolin [Verfasser]. "Die Funktion der putativen alkalischen Ceramidase ACER1 innerhalb des Sphingolipidstoffwechsels in Arabidopsis thaliana / Carolin Markus." Bonn : Universitäts- und Landesbibliothek Bonn, 2015. http://d-nb.info/1080864571/34.
Повний текст джерелаFuchs, Kathrin [Verfasser], and Johannes [Akademischer Betreuer] Kornhuber. "Charakterisierung der Ceramidase-Aktivität im Plasma gesunder und alkoholabhängiger Probanden / Kathrin Fuchs. Gutachter: Johannes Kornhuber." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2014. http://d-nb.info/1075478774/34.
Повний текст джерелаMeiners, Jana [Verfasser], and Astrid [Akademischer Betreuer] Westendorf. "The effect of the acid sphingomyelinase/ceramidase system on bacterial induced colitis / Jana Meiners ; Betreuer: Astrid Westendorf." Duisburg, 2019. http://d-nb.info/1191692159/34.
Повний текст джерелаDrosos, Zacharias [Verfasser], Lars [Akademischer Betreuer] Hanker, and Markus [Akademischer Betreuer] Meier. "Die prognostische Bedeutung der Expression von Acid-Ceramidase (ASAH1) und Sphingosin-Kinase 1 (SPHK1) bei Patientinnen mit Ovarialkarzinom / Zacharias Drosos ; Akademische Betreuer: Lars Hanker, Markus Meier." Lübeck : Zentrale Hochschulbibliothek Lübeck, 2021. http://d-nb.info/1236386272/34.
Повний текст джерелаYamakawa, Nathália Christina Gonçalves 1986. "Epilepsia e Morita-Baylis-Hillman : uma abordagem sintética para ceramidas antiepiléticas." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250239.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Química
Made available in DSpace on 2018-08-21T16:21:23Z (GMT). No. of bitstreams: 1 Yamakawa_NathaliaChristinaGoncalves_M.pdf: 16524766 bytes, checksum: 72cb2d423e322cd66bf985a8a162ae5a (MD5) Previous issue date: 2012
Resumo: A epilepsia é uma das principais doenças do sistema nervoso central, caracterizada por uma alteração na atividade elétrica do cérebro, que leva a perdas de memória e convulsões. Essa doença afeta cerca de 50 milhões de pessoas no mundo, que são discriminadas e isoladas da sociedade. Existem vários medicamentos que podem ser utilizados, no entanto, muitas vezes é necessário a administração de associações desses que apresentam severos efeitos colaterais. Esse quadro justifica a necessidade da busca por substâncias mais eficientes para o tratamento dessa doença. Em 2008, Ahmed e cols. isolaram da esponja marinha Negombata corticata duas ceramidas que apresentaram importante atividade anti-convulsiva. Assim, este trabalho teve por objetivo estabelecer uma estratégia sintética para a preparação do fragmento polar de tais ceramidas. A rota foi baseada em um aduto de Morita-Baylis-Hillman (MBH), obtido com elevada diastereosseletividade a partir de uma reação entre o aldeído de Garner e o acrilato de etila. Uma reação de ozonólise na dupla ligação do aduto de MBH fornece um a-cetoéster, cuja carbonila cetônica e reduzida conduzindo a um único produto. A proteção das hidroxilas, permitiu a confirmação da estereoquímica relativa através de análise por difração de raios-X, que também evidenciou a ocorrência de racemização na reação de MBH. O diol também foi utilizado na preparação de um aza-açúcar, potencial inibidor de glicosidase, sendo esta síntese em apenas 4 etapas com 32% de rendimento global. A fração apolar da ceramida foi sintetizada a partir de uma reação de Grignard, e a junção dos fragmentos pode ser realizada utilizando-se uma reação de Wittig. Desta maneira, foi descrita uma nova estratégia sintética que pode ser aplicada na preparação de diversos análogos das ceramidas, desenvolvendo um antiepiléptico mais potente
Abstract: Epilepsy is a chronic brain disorder that affects around 50 million people all over the world. It is characterized by recurrent seizures - which are physical reactions to sudden excessive electrical discharges in a group of brain cells. The discrimination and social stigma that surround epilepsy worldwide are often more difficult to overcome than the seizures themselves. Because of this fact and the economical impacts of the disease, the research for new biologically active compounds is still necessary. In 2008, Ahmed et al. isolated from the Red Sea sponge Negombata corticata two ceramides, which exhibit in vivo anticonvulsant activity. This work is focused on establishing of a synthetic sequence to prepare the polar fragment present in both ceramides. The strategy was based on a Morita-Baylis-Hillman reaction between a Garner¿s aldehyde and ethyl acrylate that provided a functionalized intermediate in good diastereoselectivity. The major diastereoisomer was employed as substrate in an ozonolysis reaction, followed by a stereoselective reduction that afforded 1,2-diol as a single isomer. The acetonide derived from this 1,2-diol allowed us to determine through X-Ray diffraction analysis the relative stereochemistry of this compound as being 1,2- anti. To finish the synthesis of the polar fragment, the ester group present in the acetonide was reduced to the corresponding aldehyde. The diol also was applied in a high diastereoselective preparation of an azasugar in 4 steps and 32% yield overall. In this work we also describe the synthesis of a carbon chain of the ceramide, our route includes an approach to the apolar fragment obtained by a Grignard reaction; then a Wittig reaction can couple both fragments toward the finalization of the sphingosine¿s synthesis. Our synthetic route can also be used in the preparation of several analogues of the antiepiletic ceramides
Mestrado
Quimica Organica
Mestre em Química
Day, Jenna. "Coumarinyl-Caged Ceramides, a New Tool for Assessing the Biological Effects of Ceramide In Cells." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32336.
Повний текст джерелаWijesinghe, Dayanjan. "Ceramide Kinase and Ceramide-1-Phosphate." VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/1621.
Повний текст джерелаPark, Hyejung. "Characterization of ceramide synthases (Cers) in mammalian cells." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/29616.
Повний текст джерелаCommittee Chair: Alfred H. Merrill, Jr; Committee Member: John Cairney; Committee Member: M. Cameron Sullards; Committee Member: Marion B. Sewer; Committee Member: Yuhong Fan. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Marques, Suzi Oliveira. "Estudo químico da esponja Dysidea robusta." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/75/75131/tde-11032010-111805/.
Повний текст джерелаSponges of the genus Dysidea (Order: Dyctioceratida) are characterized as sources of several biologically active secondary metabolites. This work describes the study of two samples of D.robusta, DR1 and DR2, both collected at the Bahia state coastline, in 1999. The investigation aimed the crude extract fractionation and analysis by LC-MS and by 1D and 2D NMR techniques. Among the extracts DR1, the fraction DR1-EP-5A obtained from the petroleum ether extract showed a mixture of three saturated ceramides, represented by 22, 23 and 24. From the DR2 sample, the fractions obtained from the aqueous extract DR2-AQ-6B and -6D presented pirodisinoic acid derivates 18, 19, 20 and 21. Except for pyrodisinoic acid (18), all other isolated compounds haven´t been reported in the literature yet.
Kiefer, Kerstin 1986. "Implication of the disease-associated ORMDL3 in macrophage physiology." Doctoral thesis, Universitat Pompeu Fabra, 2016. http://hdl.handle.net/10803/664118.
Повний текст джерелаAlguns estudis d’associació genòmica han relacionat un augment dels nivells d’expressió d’ORMDL3, causat per polimorfismes d’un sol nucleòtid, amb diverses malalties inflamatòries. Tot i així, els mecanismes fisiopatològics subjacents a aquesta associació no són del tot coneguts. Les proteïnes ORMDL estan involucrades en la síntesi d’esfingolípids de novo, un procés essencial per a la resposta immunitària de tipus innat i que ha estat associat amb l’asma. Aquesta tesi té l’objectiu d’aprofundir en la formació del complex ORMDL-SPT així com d’elucidar la potencial implicació d’ORMDL3 en la fisiologia dels macròfags utilitzant un model de ratolí transgènic. Els nostres resultats demostren un rearranjament estructural quan hi ha un alt contingut d’esfingolípids, per tal d’alliberar l’activitat de l’SPT. A més, els nivells d’ORMDL3 disminueixen el contingut cel·lular de ceramides i afecten específicament la síntesi de novo durant l’activació dels macròfags, produint una reducció en l’autofàgia i la liquidació bacteriana. Addicionalment, hem explorat la regulació d’ORMDL3 depenent de polimorfismes en monòcits humans. En conjunt, aquesta tesi contribueix a un coneixement més profund de la implicació d’ORMDL3 en la homeòstasi d’esfingolípids i remarca la seva importància en la fisiologia dels macròfags.
Barroso, Alvim Manuel Matos. "Esfingolípidos: mediadores moleculares da resposta celular e potenciais alvos terapêuticos." Master's thesis, [s.n.], 2012. http://hdl.handle.net/10284/3546.
Повний текст джерелаOs esfingolípidos, descritos em 1876 por J.L.W. Thudichum, são considerados constituintes essenciais das membranas das células eucarióticas. Para além do seu papel estrutural estão também envolvidos na sinalização celular. Do ponto de vista estrutural, os esfingolípidos derivam da esfingosina. A acilação da esfingosina com um ácido gordo de cadeia longa origina a ceramida, que constitui a base molecular de todos os esfingolípidos. O seu metabolismo integra vários compartimentos intracelulares que incluem o retículo endoplasmático, o complexo de Golgi, a membrana plasmática e o sistema endossomal/lisossomal. Alguns dos seus metabolitos, como a ceramida, a esfingosina, e a esfingosina-1-fosfato actuam como moléculas bioactivas, estando envolvidas em diversos eventos celulares, nomeadamente na regulação do crescimento celular, diferenciação, senescência, inflamação e apoptose. Uma das vias metabólicas mais interessantes do ponto de vista fisiológico e patológico é a via da esfingomielinase. Esta via permite regular o nível intracelular dos esfingolípidos bioactivos através da acção concertada de duas famílias de enzimas, as esfingomielinases, que convertem a esfingomielina em ceramida, e as ceramidases que convertem a ceramida em esfingosina. Cada uma destas famílias de enzimas exibe especificidade quanto ao compartimento celular/tecido e à natureza química do substrato. De facto, as células vivas contêm diversas espécies de ceramidas que diferem na extensão e grau de insaturação da cadeia acilo, o que parece ter grande importância e influência nas suas actividades biológicas. Nas últimas décadas, a diversidade estrutural e funcional dos SLs tem suscitado algum interesse, em grande parte devido ao seu envolvimento em patologias de etiologia muito diversa mas com considerável impacto em termos de Saúde Pública. Neste contexto, o presente trabalho de pesquisa bibliográfica foi elaborado com o objectivo de proporcionar uma visão relativamente integrada sobre a estrutura, função e metabolismo dos esfingolípidos em situações normais e em situações patológicas específicas, esperando-se que represente um contribuição útil para a difusão do conhecimento sobre estas multifacetadas e enigmáticas moléculas. The sphingolipids, described in 1876 by JLW Thudichum are considered essential constituents of the membranes of eukaryotic cells. In addition to its structural role, they are also involved in cell signaling. From the structural point of view, the sphingolipids are derived from sphingosine. Acylation of sphingosine with a long chain fatty acid gives rise to the ceramide, which is the molecular basis of all sphingolipids. Its metabolism comprises several intracellular compartments including the endoplasmic eticulum, the Golgi apparatus, the plasma membrane and the system endosomal / lysosomal system. Some of its metabolites, such as ceramide, sphingosine, and sphingosine 1-phosphate act as signaling and communication bioactive molecules, being involved in various cellular events, in particular in the regulation of cell growth, differentiation, senescence , inflammation and apoptosis. One of the metabolic pathways more interesting from the physiological and pathological standpoints is the metabolism of sphingomyelinase. This route is implicated in the regulation of the intracellular level of bioactive sphingolipids through the coordinated action of two families of enzymes, sphingomyelinases, which convert sphingomyelin into ceramide, and ceramidases, that convert ceramide in sphingosine. Each of these families of enzymes shows specificity regarding to the cell compartment / tissue and the chemical nature of the substrate. In fact, the living cells contain different species of ceramides which differ in length and degree of unsaturation of the acyl chain, which seems to have great importance and influence on their biological activities. The structural and functional diversity of sphingolipids has attracted some interest in recent decades, largely due to its involvement in diseases of diverse etiology, but with considerable impact on public health. In this context, a literature review on these topics was prepared aiming to provide an integrated vision of the structure, function and metabolism of sphingolipids in normal and disease states, expecting that this work represents an useful contribution to the diffusion of the knowledge about these multifaceted and enigmatic molecules.
Liu, Ying. "Regulation of ceramide and its metabolites: biosynthesis and; in situ sphingolipid analysis." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33918.
Повний текст джерелаMunick, Michael. "Stereoselektive Synthese von lipophilen Inositolen und Ceramiden." Doctoral thesis, [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=983917183.
Повний текст джерелаZankl, Claudia. "Stereoselektive Synthese von Sphingolipiden zur Inhibierung der Degranulation von Mastzellen." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-23408.
Повний текст джерелаThe present dissertation covers the synthesis of glycosphingolipids which interact with the cell membrane in order to inhibit the degranulation of mast cells. The sphingosin body was synthesized in ten steps starting from N-Boc-Serin. The key step, the glycosylation was achieved using the trichloracetimidat method. The variation of the amid sidechain, the gylcosyl headgroup and the sphingosin body created a number of derivatives that were tested in the degranulation assay
Tepper, Annemiek Delina. "The role of ceramide in apoptosis." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2000. http://dare.uva.nl/document/84270.
Повний текст джерелаSeedlock, Kyle Elizabeth. "Calcium Mediated Regulation of Ceramide Kinase." VCU Scholars Compass, 2007. http://scholarscompass.vcu.edu/etd_retro/173.
Повний текст джерелаKim, Shang-U. "Synthesis of diene ceramides with organozirconium reagents." Thesis, The University of Arizona, 2004. http://hdl.handle.net/10150/292081.
Повний текст джерелаPedrero, Cisterna Andrea R. "Efecto de ceramidas sintéticas sobre la inducción de necrosis en células HTC." Tesis, Universidad de Chile, 2005. http://repositorio.uchile.cl/handle/2250/105423.
Повний текст джерелаLos organismos regulan su número de células a través de un balance entre la división y muerte celular. Con respecto a este último, existen diversos mecanismos de muerte celular, como la apoptosis, autofagia y necrosis, presentando cada uno características morfológicas y moleculares diferentes. Se ha considerado a la necrosis como un mecanismo de muerte celular “accidental” y, por tanto, menos regulado. Sin embargo existen evidencias de que la necrosis también puede ser considerada un tipo de muerte celular programada. Es así que las ceramidas, precursores de los esfingolípidos eucariontes, han sido reconocidas como importantes segundos mensajeros implicados en el gatillamiento de procesos apoptótico/necróticos en diversos tipos celulares. Recientemente se ha descrito que células de linfoma B humano (línea celular A20) al ser estimuladas con ligando de Fas en presencia de ceramidas van a un destino de muerte necrótico. Además de esto, se ha descrito una relación entre estrés oxidativo y la generación de ceramidas, relación que se encontraría íntimamente ligada a procesos de señalización de muerte celular. Con estos datos se postuló que las ceramidas intervienen en la decisión apoptóticonecrótica en células epiteliales. Utilizando métodos de citometría de flujo y ensayos enzimáticos se encontró que las ceramidas aumentaron la muerte por necrosis en el tiempo tanto para células HeLa como para HTC, este aumento es dosis dependiente. Ácido flufenámico, un inhibidor inespecífico de canales catiónicos no selectivos involucrados en regulación del volumen celular por estrés oxidativo, en conjunto con ceramidas no cambió el porcentaje de muerte por necrosis y aumentó la población apoptótica. Al reemplazar el Na+ extracelular por el catión monovalente NMDG+ no se observó diferencia entre los porcentajes de las distintas poblaciones celulares. La aplicación en conjunto de H2O2 con ceramidas aumentó la muerte celular, pero sólo a altas concentraciones (10 mM). El efecto de ceramidas sobre la apoptosis se ensayó utilizando el inhibidor genérico de caspasas zVAD-fmk. No hubo cambios significativos en los porcentajes de muerte celular. Efectos similares se observaron al depletar los depósitos intracelulares de calcio. Estos resultados permiten concluir que las ceramidas inducen muerte celular necrótica de manera tiempo y dosis dependiente
Organisms regulate their number of cells through fine a balance between cell division and cell death. In respect to cell death, diverse mechanisms exist, such as apoptosis, autophagia and necrosis, presenting each one different morphologic and molecular characteristics. Necrosis has been considered as a mechanism of "accidental" cell death and therefore, less regulated. Nevertheless, evidences exist of which necrosis can be also considered as a type of programmed cell death. For example, ceramides, precursors of sphingolipids in euchariotic cells, have been recognized as important second messengers implicated in the development of apoptotic/necrotic processes in diverse cell types. It has been recentely described that human lymphoma B cells (A20 cell line),upon stimulation with Fas ligand in the presence of ceramides, induce necrotic cell death. Also, it has been described a relationship between oxidative stress and the generation of ceramides which are intimately linked to cell death signaling processes. Based on this information, it was postulated that ceramides take part in the apoptotic-necrotic decision in epithelial cells. Using flow citometry and enzymatic assays we found that ceramides increased cell death by necrosis in HeLa and HTC cells in a dose-dependent manner. Flufenamic acid, a non specific inhibitor of nonselective cation channels involved in regulation of the oxidative stress-dependent cell volume, used in conjunction with ceramides did not produce changes in the percentage of necrotic cell death and increased the population of apoptotic cells. Upon replacement of external Na+ by the monovalent cation NMDG+, the percentage of different cell populations was not altered. The application of ceramides with H2O2 produced an increase in the cell death, but only at high concentrations of H2O2 (10 mM). The effect of ceramides upon apoptosis was assayed using the generic caspase inhibitor zVAD- fmk. No significant changes were observed in the percentage of cell death. Similar effects were observed when depleting the intracellular calcium stores. These results allow us to conclude that ceramides induces necrotic cell death in a time and dose-dependent manner
Munick, Michael. "Stereoselektive Synthese von lipophilen Inositolen und Ceramiden." Doctoral thesis, Technische Universität Dresden, 2006. https://tud.qucosa.de/id/qucosa%3A23910.
Повний текст джерелаAlmeida, Josà Gustavo Lima de. "Constituintes QuÃmicos dos ZoantÃdeos Palythoa caribaeorum (Duchassaing & Michelotti, 1860) e Protopalythoa Variabilis (Duerden, 1898)." Universidade Federal do CearÃ, 2011. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7953.
Повний текст джерелаEste trabalho descreve a composiÃÃo quÃmica das espÃcies marinha Palythoa caribaeorum e Protopalythoa variabilis, coletadas no municÃpio de Paracuru-CE. O fracionamento cromatogrÃfico do extrato hexÃnico de P. caribaeorum, resultou no isolamento de quatro esterÃides tetracÃclico de esqueleto ergostano: 24(R)-ergost-5-en-3-ol (P-1); 5,8-epidioxi-24(R)-ergost-6-en-3-ol (P-2); 24(R)-ergost-5-en-3,7-diol (P-4) e 24(R)-7-hidroperoxi-ergost-5-en-3-ol (P-7), um derivado do glicerol, 1-O-hexadecilglicerol (P-3) e quatro ceramidas: N-(2S,3R,4E,8E,1,3-dihidroxi-4,8-octadecadieno)hexadecanamida (P-5); N-(2S,3R,4E,1,3-dihidroxi-4-octadeceno)-hexadecanamida (P-6), N-[2S,3R,4E,8E,1-(2â-metilamino-etanosulfonila)-3-hidroxi-4,8-octadecaÂdieno]hexadecanamida (P-8) e N-[2S,3R,4E,1-(2â-metilaminoetano-sulfonila)-3-hidroxi-4-octadeceno]hexadecanamida (P-9). Do fracionamento cromatogrÃfico do extrato etanÃlico, foi possÃvel isolar o esterÃide 24(R)-ergost-7-en-3,56-triol (P-10) e o nucleosÃdeo 2-metil-timidina (P-11). Do estudo quÃmico do extrato hexÃnico de P. variabilis obteve-se os mesmos constituintes quÃmicos isolados de P. caribaeorum (P-1, P-2, P-3 e P-4) e as quatro ceramidas (P-5, P-6, P-8 e P-9). AlÃm destes compostos foi isolado um Ãster de cadeia alifÃtica, hexadecanoato de nonila (P-12) e o esterÃide Ãcido 24(R)-B-norergostan-3-5-diol-6-carboxÃlico (P-13). O potencial citotÃxico e antifÃngico das ceramidas foi avaliado, entretanto, estas nÃo apresentaram atividade. Os compostos foram isolados atravÃs de cromatografia de adsorÃÃo em gel de sÃlica e cromatografia lÃquida de alta eficiÃncia. As estruturas dos compostos obtidos foram elucidadas utilizando tÃcnicas espectroscÃpicas e espectromÃtricas, tais como: espectrometria de massa acoplada a cromatografia gasosa (CG/EM); espectrometria de massa de alta resoluÃÃo (EMAR); espectroscopia na regiÃo do infravermelho (IV) e RessonÃncia MagnÃtica Nuclear (RMN 1H, 13C e 15N) atravÃs de sequÃncias de pulsos uni e bidimensionais e comparaÃÃo com dados de RMN na literatura.
This work describes the chemical composition of the marine species Palythoa caribaeorum and Protopalythoa variabilis, both collected at Paracuru beach, state of CearÃ. The cromatographic fractionation of the hexane extract from P. caribaeorum resulted in the isolation of four tetracyclic sterols possessing the ergostan skeleton: 24(R)-ergost-5-en-3b-ol (P-1); 5 a,8a-epidioxy-24(R)-ergost-6-en-3b-ol (P-2); 24(R)- ergost-5-en-3b,7a-diol (P-4) and 24(R)-7a-hydroperoxy-ergost-5-en-3b-ol (P-7), a glycerol derivative, 1-O-hexadecylglycerol (P-3) and four ceramides: N- (2S,3R,4E,8E,1,3-dihydroxy-4,8-octadecadienyl)hexadecanamide (P-5); N- (2S,3R,4E,1,3-dihydroxy-4-octadecenyl)hexadecanamide (P-6); N-[2S,3R,4E,8E,1-(2â- methylamino-ethanosulfonyl)-3-hydroxy-4,8-octadecaenyl]hexadecanamide (P-8) and N-[2S,3R,4E,1-(2â-methylaminoethano-sulfonyl)-3-hydroxy-4-octadecenyl]hexadecanamide (P-9). The cromatographic fractionation of the ethanol extract permited the isolation of a steroid, 24(R)-ergost-7-en-3b,5a,6b-triol (P-10) and a nucleoside 2- methyltimidine (P-11). Column chromatography of the hexane extract of P. variabilis led to the isolation of nonyl hexadecanoate (P-12), the sterol 24(R)-B-norergostan-3b- 5b-diol-6b-carboxylic acid (P-13) and the same chemical constituents previously isolated from P. caribaeorum (P-1, P-2, P-3 e P-4) including the four ceramides (P-5, P-6, P-8 e P-9). The citotoxic and antifungal properties of all ceramides were evaluated, nevertheless none of them showed any activity. All compounds were isolated through adsorption column cromatography over silica gel followed by high performance liquid chromatography. The structures of the isolated compounds were elucidated using spectrometric techniques, such as: GC/MS, HRESIMS, IR and NMR (1H, 13C and 15N) through 1D and 2D pulse sequences and, whenever the case, comparison with literature data
Tronco, Cristiana de Melo Trinconi. "Tamoxifeno no tratamento de leishmaniose: atividade em esquemas terapéuticos combinados e estudo do mecanismo de ação." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-19042016-171456/.
Повний текст джерелаLeishmaniasis is a parasitic disease with wide distribution and limited treatment. Recent reports demonstrate that tamoxifen is an effective drug for experimental leishmaniasis treatment. In this work, we evaluated the combined therapy of tamoxifen with current drugs used in leishmaniasis chemotherapy. The drug interaction was additive both, in vitro and in vivo. We also evaluated tamoxifen effect on in Leishmania sphingolipids biosynthesis. We found a reduction in phosphatidylinositol and inositol phosphorylceramide (IPC) synthesis and an accumulation of acilceramide. The reduction in IPC biosynthesis could not be assigned to the reduction observed in inositol transport, but probably is related to IPC synthase inhibition. These results show new strategies to circumvent shortcomings of leishmaniasis treatment using tamoxifen, a multitarget drug in Leishmania and widely used in the chemotherapy of breast cancer.
Turnbull, Kenneth James. "The effect of Daunorubicin on human lymphoblastic leukaemia cells and the role of ceramide and ceramide-sensitive protein kinases." Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312330.
Повний текст джерелаLau, Kent G. "Formulation of novel double-chain lipid vesicles." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272376.
Повний текст джерелаSubramanian, Preeti. "Role of Ceramide-1-Phosphate as a Specific and Potent Activator of Group IVA Cytosolic Phospholipase A2 Alpha." VCU Scholars Compass, 2007. http://hdl.handle.net/10156/1404.
Повний текст джерелаLópez, Crisosto Camila. "Regulación de la respuesta a insulina por ceramidas en el cardiomiocito a nivel de la dinámica mitocondrial." Tesis, Universidad de Chile, 2012. http://repositorio.uchile.cl/handle/2250/113540.
Повний текст джерелаMemoria para optar al Título de Bioquímica
La obesidad y la diabetes son condiciones altamente prevalentes que representan un importante factor de riesgo para el desarrollo de patologías cardiovasculares, principal causa de muerte entre los pacientes diabéticos. La lipotoxicidad y las alteraciones metabólicas juegan un papel fundamental en la resistencia a la hormona insulina y el daño cardiaco en estos pacientes. Los cardiomiocitos son las unidades funcionales del corazón y poseen un alto requerimiento energético que depende en su mayor parte de la función mitocondrial. Las mitocondrias forman una red dinámica que se remodela constantemente por eventos de fisión y fusión. La mantención de una morfología mitocondrial balanceada es fundamental para mantener una funcionalidad adecuada de este organelo. El objetivo de este trabajo fue investigar el efecto de las ceramidas, que derivan del metabolismo lipídico, en la señalización de la insulina y la dinámica mitocondrial en cultivos primarios de cardiomiocitos de rata. La señalización de insulina se evaluó mediante Western blot para Akt fosforilada y la morfología mitocondrial por microscopía confocal en células teñidas con Mitotracker Green. El tratamiento de los cardiomiocitos con C2-ceramida (40 μM, 3 h) disminuyó la fosforilación de Akt basalmente y en respuesta a insulina y favoreció la fisión mitocondrial, aumentando la translocación de la proteína de fisión Drp-1 hacia este organelo. Para evaluar si ambos efectos estaban relacionados, se inhibió la actividad de Drp-1 mediante el uso de un dominante negativo y de un inhibidor químico, antes del tratamiento con C2-ceramida. La inhibición de Drp-1 mediante ambas herramientas previno la fisión mitocondrial causada por C2-ceramida y rescató la fosforilación de Akt en respuesta a insulina. Trabajos previos de nuestro laboratorio muestran que el tratamiento de los cardiomiocitos con palmitato 500 μM durante 3 h también induce fisión de la red mitocondrial. En este trabajo se mostró que al inhibir la síntesis de ceramidas a partir de palmitato se previene, en parte, los efectos de este ácido graso sobre la dinámica mitocondrial de los cardiomiocitos. En conclusión, la fragmentación de la red mitocondrial inducida por ceramidas es necesaria para la disminución de la señalización de insulina en los cardiomiocitos. Además, la fisión mitocondrial inducida por palmitato en este modelo depende en parte de la generación de ceramidas.
Obesity and diabetes are highly prevalent conditions that represent an important risk factor for the development of cardiovascular diseases, the main cause of death in diabetic patients. Lipotoxicity and metabolic alterations take part in insulin resistance and heart damage in these patients. Cardiomyocytes are the functional basic units of the heart and have a high energy requirement that depends largely on mitochondrial function. Mitochondria form a dynamic network that is constantly remodelled by fission and fusion events. The maintenance of a balanced mitochondrial morphology is critical to maintain a proper functionality of this organelle. The aim of this study was to investigate the effect of ceramides, derived from lipid metabolism, in insulin signalling and mitochondrial dynamics in primary cultures of rat cardiomyocytes. Insulin signalling was assessed by Western blot for phosphorylated Akt and mitochondrial morphology by confocal microscopy in Mitotracker Green-stained cells. Treatment of cardiomyocytes with C2-ceramide (40 μM, 3 h) decreased the phosphorylation of Akt at baseline and in response to insulin and induced mitochondrial fission, increasing the translocation of the fission protein Drp-1 to this organelle. To assess whether both effects were related, Drp-1 activity was inhibited by using a dominant negative and a chemical inhibitor, before treatment with C2-ceramide. The inhibition of Drp-1 by both tools prevented mitochondrial fission caused by C2-ceramide and rescued Akt phosphorylation in response to insulin. Previous work in our laboratory showed that treatment of cardiomyocytes with palmitate 500 μM for 3 h also induces mitochondrial fission. We showed that inhibiting the synthesis of ceramides from palmitate prevented in part the effects of this fatty acid on mitochondrial dynamics in cardiomyocytes. In conclusion, the mitochondrial network fragmentation induced by ceramides is required for the decrease of insulin signalling in cardiomyocytes. Furthermore, palmitate-induced mitochondrial fission in this model depends in part on the generation of ceramides.
Fondap, Fondecyt
Fletcher, Sarah Jayne. "Investigating the role of ceramides in plant signal transduction." Thesis, Lancaster University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.436752.
Повний текст джерелаSouza, Sofia Leite. "On the physical-chemical properties of ceramide C16." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2012. http://hdl.handle.net/10362/12024.
Повний текст джерелаCeramides are known to be involved in cell signalling and are proposed to assist in the formation of laterally segregated membrane domains, known as ceramide rich domains in cell lipid bilayers. The lipid matrix of the stratum corneum, the uppermost layer of the skin, which is responsible for its water barrier properties, is mainly composed of ceramides, associated with cholesterol, long chain fatty acids and cholesteryl esters.(...)
Sawai, Hirofumi. "Role for ceramide in apoptosis of leukemia cells." Kyoto University, 1997. http://hdl.handle.net/2433/202164.
Повний текст джерелаHansen, Melissa Ellen. "The Role of Ceramides in Mediating Endotoxin-Induced Mitochondrial Disruption." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5495.
Повний текст джерелаSridevi, Priya Alexander Hannah Ben-Ze'ev Alexander Stephen. "Regulation of ceramide synthase 1 in cellular stress response." Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/6690.
Повний текст джерелаHolopainen, Juha. "Ceramide - a messenger of cell death : a biophysical approach." Helsinki : University of Helsinki, 2001. http://ethesis.helsinki.fi/julkaisut/laa/biola/vk/holopainen/.
Повний текст джерелаSant'anna, Ana Luisa Silva. "Estudo da deposição de ceramidas sobre a fibra capilar para o combate a danos cuticulares." [s.n.], 2000. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250433.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica
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Mestrado
Thatcher, Mikayla Orton. "HMGB1 and Ceramides: Potential Mediators of Cigarette Smoke-induced Metabolic Dysfunction." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/6041.
Повний текст джерелаIzquierdo, García Eduardo. "Chemical approaches to the study of the ceramide synthase activity." Doctoral thesis, Universitat de Barcelona, 2020. http://hdl.handle.net/10803/671426.
Повний текст джерелаLos esfingolípidos (SLs) son una de las principales categorías de lípidos presentes en los organismos eucariotas. Los SL no sólo son componentes estructurales esenciales de las membranas celulares, sino que también actúan como moléculas señalizadoras. Las ceramidas (Cer) son una tipología de SL que están formadas por una base esfingoide y una cadena de ácido graso de longitud variable unidos a través de un enlace amida. Las Cer participan como segundos mensajeros en procesos celulares como la apoptosis, la autofagia, la diferenciación celular y la senescencia. Las ceramida sintasas (CerS) son un grupo de enzimas del retículo endoplasmático que catalizan la N-acilación de bases esfingoides, como la esfingosina, utilizando acil-CoAs de distintas longitudes, para dar Cer. En los mamíferos se han descrito seis isoformas de la CerS y cada una de ellas tiene preferencia por un pequeño grupo de ácidos grasos de longitud de cadena definida, por lo que cada una produce perfiles de Cer característicos. En los últimos años se ha visto que la longitud de la cadena acilo de las Cer influye en sus propiedades biofísicas y en las cascadas de señalización en las que participan. Además, se sabe que ciertas Cer están involucradas en el desarrollo de distintas enfermedades como el cáncer, la diabetes, el Alzheimer o la esclerosis múltiple. En este sentido, el desarrollo de herramientas adecuadas para el estudio de la actividad de CerS es fundamental para descifrar los mecanismos moleculares a través de los cuáles actúan las Cer, siendo este el objetivo principal que se persiguió en la presente tesis doctoral. La primera parte de la tesis se centró en el desarrollo de un nuevo ensayo para determinar la actividad CerS por medio del fenómeno de FRET. Para ello, se diseñaron y sintetizaron una serie de sondas esfingoides derivadas de la espisulosina, una pequeña quimioteca de análogos de ácidos grasos “clicables” de distintas longitudes de cadena y una colección de reactivos fluorescentes marcados con un grupo biciclo[6.1.0]nonino (BCN) o 1,2,4,5-tetrazina (Tz). Las propiedades de absorción y emisión de fluorescencia de estos compuestos fueron estudiadas en varios disolventes a través de experimentos “en cubeta”. En base a estos experimentos anticipamos que las parejas de fluoróforos seleccionadas eran adecuadas para su uso en experimentos de FRET. A continuación, se evaluó la incorporación metabólica de las distintas sondas esfingoides y de los varios análogos de ácidos grasos en medios biológicos. Los estudios de lipidómica mostraron que tanto las sondas como los análogos de ácidos grasos eran procesados por las CerS para dar las Cer correspondientes. Sin embargo, los análogos de ácidos grasos también entraron en otras rutas metabólicas de los lípidos dando lugar a un elevado ruido de fondo tras la reacción de marcaje de fluorescencia. Nuestros intentos para solventar este problema fueron en vano y, por tanto, finalmente no fue posible la implementación del ensayo de fluorescencia para medir la actividad CerS. En la segunda parte de la tesis se propuso el desarrollo de nuevos CLIPTACs dirigidos a la degradación de CerS, como alternativa a los inhibidores clásicos para la modulación de la actividad CerS. Para ello se diseñaron y sintetizaron cuatro derivados de BCN que contuvieran un ligando para reclutar distintos enzimas E3 ligasas de ubiquitina. Estos reclutadores de E3 ligasa serán utilizados en un futuro en combinación con un derivado de la Jaspina B, un análogo del sustrato de las CerS, para obtener los CLIPTACs deseados.
Stiban, Johnny Pierre. "Ceramide metabolism and transport implications on the initiation of apoptosis /." College Park, Md. : University of Maryland, 2006. http://hdl.handle.net/1903/6658.
Повний текст джерелаThesis research directed by: Biology. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
Sociale, Mariangela [Verfasser]. "Ceramide Synthase in Transcriptional Regulation and Lipid Sensing / Mariangela Sociale." Bonn : Universitäts- und Landesbibliothek Bonn, 2019. http://d-nb.info/1188731017/34.
Повний текст джерелаNarita, Keishi. "Study of ceramide glucosyltransferase : mechanism of inhibition by imino sugars." Thesis, University of Oxford, 2001. http://ora.ox.ac.uk/objects/uuid:a96eb6ad-9058-485f-91c3-d7cde9762e81.
Повний текст джерелаTrinconi, Cristiana de Melo. "Investigação sobre a atividade de Ceramida Sintase em Leishmania amazonensis." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-13022012-091618/.
Повний текст джерелаLeishmaniasis is a widely distributed parasitic disease with difficult treatment. The leishmanicidal activity of tamoxifen was recently identified, leading to its proposal as an alternative treatment for leishmaniasis. In this work, we aimed at characterizing the activity of ceramide synthase (CerS) in L. amazonensis and testing whether this enzymatic activity in inhibited by tamoxifen. We have identified, in the L. amazonensis genome, an ORF which encodes a protein similar to the Saccharomyces cerevisiae CerS, with six transmembrane domains and a characteristic Lag1 motif. The characterization of the in vitro enzymatic activity showed that the enzyme recognizes sphingosine/sphinganine as well as palmitoyl/miristoyl CoA as precursors. This enzyme is not sensitive to fumonisin B2 or tamoxifen. This indicates that this drug does not act through the inibition of CerS. The complete characterization of this enzyme will provide valuable information about the sphingolipid methabolism of these protozoa.
Hodson, Aimee Elizabeth. "Insulin Treatment Increases Myocardial Ceramide Accumulation and Disrupts Cardiometabolic Function." BYU ScholarsArchive, 2016. https://scholarsarchive.byu.edu/etd/5954.
Повний текст джерелаTippetts, Trevor Stanley. "Cigarette Smoke Increases Cardiomyocyte Ceramide Accumulation and Inhibits Mitochondrial Respiration." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5596.
Повний текст джерелаDupre, Tess V., Mark A. Doll, Parag P. Shah, Cierra N. Sharp, Deanna Siow, Judit Megyesi, James Shayman, et al. "Inhibiting glucosylceramide synthase exacerbates cisplatin-induced acute kidney injury." AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2017. http://hdl.handle.net/10150/624924.
Повний текст джерелаBIZOT, FOULON VALERIE. "Influence des ceramides d'origine vegetales sur la regulation de l'expression et de l'activite des proteinases neutres : encapsulation de l'acide all-trans retinoique et effet intrinseque des ceramides." Paris 11, 1995. http://www.theses.fr/1995PA114838.
Повний текст джерелаIto, Mitsuru. "Possible role of ceramide as an indicator of chemoresistance : decrease of the ceramide content via activation of glucosylceramide synthase and sphingomyelin synthase in chemoresistant leukemia." Kyoto University, 2003. http://hdl.handle.net/2433/148774.
Повний текст джерелаTomonaga, Nami. "Studies on marine sphingophosphonolipids as new food ingredients." Kyoto University, 2019. http://hdl.handle.net/2433/242681.
Повний текст джерела0048
新制・課程博士
博士(農学)
甲第21804号
農博第2317号
新制||農||1065(附属図書館)
学位論文||H31||N5176(農学部図書室)
京都大学大学院農学研究科応用生物科学専攻
(主査)教授 菅原 達也, 教授 佐藤 健司, 教授 松井 徹
学位規則第4条第1項該当
Ramírez, Flores Sara. "Hypothalamic Ceramide Levels regulated by CPT1C mediate the Orexigenic effect of Ghrelin." Doctoral thesis, Universitat Internacional de Catalunya, 2014. http://hdl.handle.net/10803/276184.
Повний текст джерела