Добірка наукової літератури з теми "Cellules souches humaines induites à la pluripotence"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Cellules souches humaines induites à la pluripotence".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Статті в журналах з теми "Cellules souches humaines induites à la pluripotence":
Ahmed, E., M. Fieldes, C. Bourguignon, J. Mianné, A. Nasri, A. Petit, I. Vachier, S. Assou, A. Bourdin, and J. De Vos. "Caractérisation d’un épithélium bronchique dérivé de cellules souches pluripotentes induites humaines (iALI)." Revue des Maladies Respiratoires 38, no. 6 (June 2021): 578. http://dx.doi.org/10.1016/j.rmr.2021.02.021.
Bohl, Delphine. "Les cellules neuronales dérivées des cellules souches pluripotentes induites humaines : modélisation des maladies du motoneurone." Biologie Aujourd'hui 210, no. 1 (2016): 27–36. http://dx.doi.org/10.1051/jbio/2016004.
De Vos, John, Mathieu Fiedles, Chloé Bourguignon, Joffrey Mianne, Engi Ahmed, Isabelle Vachier, Arnaud Bourdin, and Said Assou. "Modélisation de l’épithélium bronchique à partir de cellules souches humaines pluripotentes induites (iPSC)." Morphologie 103, no. 342 (November 2019): 88. http://dx.doi.org/10.1016/j.morpho.2019.10.029.
Fieldès, M., E. Ahmed, C. Bourguignon, J. Mianné, C. Vernisse, A. Fort, I. Vachier, A. Bourdin, S. Assou, and J. De Vos. "Modélisation de l’épithélium bronchique dans la bronchopneumopathie chronique obstructive par les cellules souches pluripotentes induites humaines." Revue des Maladies Respiratoires 37, no. 3 (March 2020): 197–200. http://dx.doi.org/10.1016/j.rmr.2020.02.003.
Nasri, A., F. Foisset, C. Bourdais, E. Ahmed, I. Vachier, S. Assou, A. Bourdin, and J. De Vos. "Compartiment épithélial et mésenchymateux lors de la différenciation de cellules souches pluripotentes humaines induites en épithélium bronchique." Revue des Maladies Respiratoires 40, no. 2 (February 2023): 117. http://dx.doi.org/10.1016/j.rmr.2022.11.018.
Foisset, F., C. Lehalle, A. Nasri, C. Bourdais, I. Vachier, S. Assou, Q. Muller, et al. "Développement d’un modèle d’épithélium bronchique innervé par des neurones sensitifs à partir de cellules souches pluripotentes induites humaines (iPSCs)." Revue des Maladies Respiratoires 40, no. 2 (February 2023): 111. http://dx.doi.org/10.1016/j.rmr.2022.11.006.
Afanassieff, Marielle, Irène Aksoy, Nathalie Beaujean, Pierre-Yves Bourillot, and Pierre Savatier. "Cinquante nuances de pluripotence." médecine/sciences 34, no. 11 (November 2018): 944–53. http://dx.doi.org/10.1051/medsci/2018240.
Jmel Boyer, Inès, and Emmanuel García Sánchez. "Le développement embryonnaire pré-gastrulatoire humain : modèles d’avenir et enjeux sociétaux." Biologie Aujourd’hui 214, no. 3-4 (2020): 109–13. http://dx.doi.org/10.1051/jbio/2020012.
Collin de l’Hortet, Alexandra, and Hélène Gilgenkrantz. "L’émergence des modèles miniatures de foie gras humain en 3D générés en laboratoire." médecine/sciences 36, no. 3 (March 2020): 261–63. http://dx.doi.org/10.1051/medsci/2020027.
Sansac, Caroline, Said Assou, Julien Bouckenheimer, Jean-Marc Lemaître, and John De Vos. "Les cellules souches pluripotentes induites : un nouveau paradigme pour l’étude des tissus humains." Biologie Aujourd'hui 210, no. 1 (2016): 1–8. http://dx.doi.org/10.1051/jbio/2016013.
Дисертації з теми "Cellules souches humaines induites à la pluripotence":
Jung, Laura. "Optimisation de protocoles de reprogrammation de cellules somatiques humaines en cellules souches à pluripotence induite (hiPSC)." Thesis, Strasbourg, 2013. http://www.theses.fr/2013STRAJ066.
In 2006 and 2007, Yamanaka and Thomson teams achieved the reprogramming of mouse and human somatic cells into pluripotent stem cells through the transfection of two cocktails of genes: OCT4, SOX2, KLF4, cMYC (OSKM) and OCT4, NANOG, SOX2, LIN28 (ONSL). The generated cells, called induced Pluripotent Stem Cells (iPSC) share the same fundamental properties of ESC : self-renewing, pluripotency maintenance and capacity of differentiation into the three germ layers and suggest the same application potential in basic research (developmental and epigenetic biology) as well as in therapy (regenerative medicine, disease modeling for drug development). One of the major advantages of iPSC lies in their non-embryonic origin. Indeed, the use of iPSC resolves the ethical constraints and offers the possibility to work with extensive cell types directly from the patient to treat. Stéphane Viville’s research team aims to develop a hiPSC bank from patient suffering from genetic or other diseases which will be available for the scientific community. We are experienced in human primary fibroblasts reprogramming especially with the use of two polycistronic cassettes: ONSL encoding Thomson’s cocktail and OSKM encoding Yamanaka’s cocktail separated with 2A peptides. Thanks to the combination of RV-ONSL and RV-OSKM retroviral vectors (developed with Vectalys) we are yielding more than 2% of reprogramming efficiency in a highly reproducible way. Indeed, we demonstrated the reprogramming synergy of ONSL and OSKM combination. We are now focusing our effort on non-integrative strategies (ie mRNA) which are more appropriate for clinical usage
Hoarau, Priscilla. "Obtention de cellules souches humaines induites à la pluripotence à partir de cellules d'urine et leur différenciation neuronale." Master's thesis, Université Laval, 2017. http://hdl.handle.net/20.500.11794/27912.
Human Induced Pluripotent Stem Cells (hiPSCs) were conceived for the first time in 2007 in Japan, by Doctor Yamanaka’s team. These are somatic cells reprogrammed thanks to a retrovirus allowing, for example, neuronal differentiation for the purpose of neurodevelopmental disorders studies such as Schizophrenia. Today, the removal of somatic cells is mainly made by enough invasive methods, including skin and blood biopsies. This can represent a brake in their use predominantly children, mainly sick children. The preferred neuronal differentiation is the dopaminergic (DA) way because it's the mostly cell type affected in schizophrenics. That's why we prioritize the use of urine cells for this project, reprogrammed via a non integrative virus, the Sendai virus (SeV). The neuronal differentiation enables us to get functional DA neurons characterized by electrophysiology. Experimentations show a huge efficiency of urine cells reprogramming as well as a great potential of neuronal differentiation despite some distinctions between the two lines. Thanks to this project, the achievement of a cellular model for Schizophrenia could be established. The differences noticed between the two lines during the differentiation open up a new way to make cellular and molecular studies of this disease deeper.
Hafner, Anne-Laure. "Étude des progéniteurs adipeux dérivés des cellules souches pluripotentes induites humaines." Thesis, Nice, 2015. http://www.theses.fr/2015NICE4062.
In mammals, two types of adipose tissue coexist: the white (WAT) wich is involved in energy storage and the brown (BAT) which is specialized in energy expenditure. Beige adipocytes have recently been described as brown –like adipocytes and represent a third type of adipocytes that are recruited in WAT. The molecular mechanisms involved in the generation of these different types of adipocytes remains unknow in humans, mainly because of the lack of appropriate in vitro cellular models. The human induced Pluripotent Stem (hips) cells are a good model to study the earliest steps of human adipogenesis. We have shown that the generation of white and brown adipocytes progenitors (AP) is regulated by acid retinoic signaling pathway during hips cells differentiation. Functional experiments indicated that the transcription factor Pax3 is a molecular mediator of the brown phenotype. During this study, we could see that AP derived from hips cells display a low adipogenic capacity as compared to progenitors derived from adult adipose tissue. We show in this work that treatment with TGFβ pathway inhibitor SB431542 together with ascorbic acid, hydrocortisone and EGF promoted differentiation of non- genetically modified hiPSCs-BAPs at a high rate. During preliminary results, we have analyzed the role of the transcription factor Hoxc8 on PA differentiation. The surexpression of this factor lead to distinct answers on the phenotype and differentiation between hiPSCs-AP and adult-derived AP
Hafner, Anne-Laure. "Étude des progéniteurs adipeux dérivés des cellules souches pluripotentes induites humaines." Electronic Thesis or Diss., Nice, 2015. http://www.theses.fr/2015NICE4062.
In mammals, two types of adipose tissue coexist: the white (WAT) wich is involved in energy storage and the brown (BAT) which is specialized in energy expenditure. Beige adipocytes have recently been described as brown –like adipocytes and represent a third type of adipocytes that are recruited in WAT. The molecular mechanisms involved in the generation of these different types of adipocytes remains unknow in humans, mainly because of the lack of appropriate in vitro cellular models. The human induced Pluripotent Stem (hips) cells are a good model to study the earliest steps of human adipogenesis. We have shown that the generation of white and brown adipocytes progenitors (AP) is regulated by acid retinoic signaling pathway during hips cells differentiation. Functional experiments indicated that the transcription factor Pax3 is a molecular mediator of the brown phenotype. During this study, we could see that AP derived from hips cells display a low adipogenic capacity as compared to progenitors derived from adult adipose tissue. We show in this work that treatment with TGFβ pathway inhibitor SB431542 together with ascorbic acid, hydrocortisone and EGF promoted differentiation of non- genetically modified hiPSCs-BAPs at a high rate. During preliminary results, we have analyzed the role of the transcription factor Hoxc8 on PA differentiation. The surexpression of this factor lead to distinct answers on the phenotype and differentiation between hiPSCs-AP and adult-derived AP
Martineau, Sabrina. "Etude des mécanismes moléculaires de l'épidermolyse bulleuse simple à partir de cellules souches humaines induites à la pluripotence." Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASQ020.
Epidermolysis bullosa simplex (EBS) is a skin disorder caused mainly by dominant mutations in genes coding for keratin 5 (KRT5) or 14 (KRT14) genes. It is characterized by the presence of blisters caused by epidermal detachment, and by other complications such as cutaneous inflammation. From a genetic point of view, the mutations will alter the assembly of the keratin intermediate filament network in basal keratinocytes of the epidermis, leading to cell cytolysis and the formation of intra-epidermal blisters. Currently no effective therapeutic approach it is available. Understanding of the disease and the development of therapies have been hampered by the lack and limitations of relevant human cell and mouse models.So, the general aim of my thesis was to exploit the properties of human induced pluripotent stem cells (hiPSc) to modelling EBS. For this purpose, we generate hiPSc-derived keratinocytes from EBS patients carrying KRT5 mutations (Ker-EBS), and from healthy patients (Ker-WT). Comparison of Ker-EBS and Ker-WT enabled to show that Ker-EBS recapitulates the main phenotypes associated with EBS, namely decreased cell proliferation, increased cell migration, altered signalling pathways (ERK and JNK), as well as aggregates of intermediate keratin filaments in the cytoplasm, as observed in primary EBS keratinocytes. These results demonstrate that our hiPSc-derived cell model is relevant for study EBS.In order to identify new molecular mechanisms, a trancriptomic analysis comparing Ker-EBS with Ker-WT revealed 138 deregulated genes, revealing an enrichment in processes linked to the extracellular matrix, DNA packaging and the inflammatory response. As the inflammatory component in EBS has been poorly described, my next step was to study the pro-inflammatory cytokine phenotype. Thus, we were able to demonstrate increased expression of IL-1α, IL-1β, IL-6, IL-8 (CXCL8), CXCL5, CXCL10, CXCL11, CCL5 in Ker-EBS, at RNA level under basal or IFNy-stimulated conditions to mimic a pro-inflammatory context. Only the chemokines CXCL10 and CXCL11 are secreted at high concentrations in the culture supernatants of stimulated and unstimulated Ker-EBS, demonstrating the involvement of these cytokines in EBS.In parallel, in order to avoid biases due to genetic background, gender, patient age and epigenetics, we generated an isogenic Ker-EBS line (corrected Ker-EBS) using the CRISPR-Cas9 technique. We were thus able to demonstrate that the corrected Ker-EBS line showed a restoration of the expression level of the pro-inflammatory cytokines mentioned above, to a level close to that of Ker-WT, confirming a direct link between mutations in the KRT5 gene and the pro-inflammatory signature.In conclusion, our new cellular model enabled us to reproduce the pathological phenotypes known in the literature, and to demonstrate deregulation of pro-inflammatory cytokine expression in EBS, notably CXCL10 and CXCL11. Taken together, these results make this model a relevant tool to allow a better understanding of the molecular mechanisms associated with the pathology, particularly the inflammatory component, paving the way for new therapeutic approaches
Lemonnier, Thomas. "Modélisation de maladies neurodégénératives à l’aide de cellules souches pluripotentes induites humaines." Thesis, Paris 5, 2012. http://www.theses.fr/2012PA05T074/document.
Reprogramming technology of somatic cells in induced pluripotent stem cells (iPS) now offers the opportunity to model neurodegenerative diseases and to study patient’s neurons. We used this technology for generating two models of neurodegenerative diseases: the muccopolysaccharidosis type IIIB (MPSIIIB) and the ALS2 form of amyotrophic lateral sclerosis (ALS). In the MPSIIIB model, we have shown that iPS and neurons of patients had characteristic defects of the disease such as the accumulation of storage vesicles. Alterations of the Golgi apparatus in these cells were also highlighted. Transcriptome analysis of MPSIIIB neural precursors showed transcriptional changes involving particularly genes implicated in cell-extracellular matrix interactions. Thus, in a subsequent study, alterations of migration and orientation of MPSIIIB mutant mouse cells and MPSIIIB patients’ cells have been demonstrated. These alterations may be responsible for the disruption of neurogenesis and neuritogenesis in sick children. In the ALS2 model, we have shown that patients’ neurons had defects including decreased endosomes’ surface and abnormal neurite outgrowth. As there was previously no relevant cellular model reproducing the disease, this model will now allow the study of physiopathological processes involved in the disease. In conclusion, the generation of iPS cells allows to model neurodegenerative diseases and to study associated physiopathological processes on cultured human neurons. These cell models could allow in the near future the screening of molecules of potential therapeutical interest
Lemonnier, Thomas. "Modélisation de maladies neurodégénératives à l'aide de cellules souches pluripotentes induites humaines." Phd thesis, Université René Descartes - Paris V, 2012. http://tel.archives-ouvertes.fr/tel-00806699.
Lay, Russo Nadège. "Différenciation des cellules souches embryonnaires murines et des cellules souches pluripotentes induites humaines en cellules dendritiques : cellules d'intérêt pour les tests de toxicologie." Nice, 2012. http://www.theses.fr/2012NICE4077.
The seventh amendment in the European cosmetic directive imposes an abandonment of the tests on animals to measure the allergenic or irritant effects of some compounds used in cosmetic. The allergenic response in animals ‘models includes five aspects but in the in vitro test each aspect is studied separately. Among the in vitro tests of toxicity which are envisaged, dendritic cells, which are antigen presenting cells, were revealed as cells of choice for study one of these aspects. However today it is difficult to obtain a reliable and strong source of dendritic cells allowing working out a reglementary test. The aim of my thesis project was to propose an alternative model in these tests on animals. For it we set up the conditions allowing generating dendritic cells derived of stern cells. For it we have two sources of stem cells (hiPS) which having all the characteristics of the embryonic stem cells without raise ethical problems. These two types of cells allowed having an inexhaustible and plentiful source of dendritic cells. We set up one protocol allowing generating and purifying a population of dendritic cells from mouse embryonic stem cells. Cells were characterized by gene expression like CD45, CD86. Furthermore we realized as functional test that consists to measure the dextran-FICT endocytosis and the answer of this cellular population to allergenic reference molecules such as MCI/MI or TNBS. We also tried to generate “dendritic-like” cells from human iPS based to expression of specifics markers as CD45, CD34, CD1a, CD14, CD209, CD207, CD86 and HLA-DR. Several protocols were envisaged. However dendritic-like cells obtained represent a low percentage of differentiated cells and the protocol is in the course of optimization. Increasingly tests use keratinocytes cells for evaluate another aspect of allergenic response. So we were interesting also to these cells and we will present first steps differentiation of hiPS that will allow generating keratinocytes
Denis, Jérôme Alexandre. "Applications médicales et pharmaceutiques des cellules souches pluripotentes : vers un changement de paradigme ?" Phd thesis, Université René Descartes - Paris V, 2011. http://tel.archives-ouvertes.fr/tel-00637075.
Mohsen-Kanson, Tala. "Cellules souches induites à la pluripotence : modèle d'étude des étapes précoces du développement adipocytaire humain." Nice, 2012. http://www.theses.fr/2012NICE4023.
The terminal steps of adipocyte differentiation are well established ; however the earliest steps controlling brown and white adipogenic lineage specification remain unknown in humans. We have investigated the human induced Pluripotent Stem cells (hips) as a model to study the early steps of brown and white adipogenesis. We will present the generation of hips cells from human multipotent adipose derived stem cells (hMADS). We have also used iPS cells reprogrammed from human neural stem cells generated using the PiggyBac technology. We provide an efficient protocol to differentiate iPS-hMADS and iPS-hNSC cells into adipocytes. Interestingly, our data show that hips cells are able to differentiate both into white and brown adipocytes. We show that Retinoic Acid (RA) pathway activation at an early phase of hips development dramatically enhanced generation of white adipocytes and inhibited generation of Brown adipocytes. In contrast, the use of SB431542, a selective inhibitor of TGFβ/Activin pathway, indicated that this pathway was required for the generation of brown adipocytes. Altogether, these data support a model in which brown and white adipocytes progenitors diverge early during human embryonic development. RA and TGFβ/Activin pathways regulate the generation of white APs and brown APs respectively