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1
COSTA, CÉSAR S. B., JULIANO R. M. VICENTI, JOAQUÍN A. MORÓN-VILLARREYES, SERGIANE CALDAS, LIZIANE V. CARDOSO, RICARDO F. FREITAS, and MARCELO G. M. D'OCA. "Extraction and characterization of lipids from Sarcocornia ambigua meal: a halophyte biomass produced with shrimp farm effluent irrigation." Anais da Academia Brasileira de Ciências 86, no. 2 (June 2014): 935–43. http://dx.doi.org/10.1590/0001-3765201420130022.
Повний текст джерелаАнотація:
Sarcocornia ambigua is a perennial glasswort, native of South America and a potential new seed-oil crop and forage for direct irrigation with salt water. Small seeds develop inside fertile segments of its cylindrical leafless shoots and, at the harvest, seeds are typically mixed with remnant cellulose material difficult to separate. This work evaluated different extraction methods and the composition of total esterified fatty acids in a meal of ground fertile shoots of S. ambigua, seeking for an alternative primary matter and larger yield of total lipids. The highest lipid yield was obtained with a chloroform:methanol mixture (2:1)(v/v) (5.2% of dry weight). The most abundant polyunsaturated fatty acids in the meal were linoleic acid (C18:2; 21.4%) and oleic acid (C18:1; 18.3%). Fifty six percent of the lipids in S. ambigua meal were saturated and palmitic acid (C16:0) was the main fraction (19.8%). Long-chain fatty acids (≥ C20) represented 29.5% of the lipids. Most abundant long-chain fatty acids were behenic acid (C22:0; 7.1%), lignoceric acid (C24:0; 5.3%) and montanic acid (C28:0; 4.0%). The percentage of saturated lipids in S. ambigua meal was higher than that of vegetable oils with a MUFA nutritional profile and some of these lipids have known bioactive properties.
2
Markowski, Adam R., Agnieszka U. Błachnio-Zabielska, Karolina Pogodzińska, Anna J. Markowska, and Piotr Zabielski. "Diverse Sphingolipid Profiles in Rectal and Colon Cancer." International Journal of Molecular Sciences 24, no. 13 (June 29, 2023): 10867. http://dx.doi.org/10.3390/ijms241310867.
Повний текст джерелаАнотація:
Colorectal cancer is a heterogenous group of neoplasms showing a variety of clinical and pathological features depending on their anatomical location. Sphingolipids are involved in the formation and progression of cancers, and their changes are an important part of the abnormalities observed during carcinogenesis. Because the course of rectal and colonic cancer differs, the aim of the study was to assess whether the sphingolipid profile is also different in tumors of these two regions. Using a combination of ultra-high-performance liquid chromatography combined with triple quadrupole mass spectrometry, differences in the amounts of cellular sphingolipids were found in colorectal cancer. Sphingosine content was higher in rectal cancer than in adjacent healthy tissue, while the content of two ceramides (C18:0-Cer and C20:0-Cer) was lower. In colon cancer, a higher content of sphingosine, sphinganine, sphingosine-1-phosphate, and two ceramides (C14:0-Cer and C24:0-Cer) was found compared to healthy tissue, but there was no decrease in the amount of any of the assessed sphingolipids. In rectal cancer, the content of sphinganine and three ceramides (C16:0-Cer, C22:0-Cer, C24:0-Cer), as well as the entire pool of ceramides, was significantly lower compared to colon cancer. The S1P/Cer ratio in rectal cancer (S1P/C18:1-Cer, S1P/C20:0-Cer, S1P/C22:0-Cer, S1P/C24:1-Cer) and in colon cancer (S1P/C18:0-Cer, S1P/C18:1-Cer, S1P/C20:0-Cer) was higher than in adjacent healthy tissue and did not differ between the two sites (rectal cancer vs. colonic cancer). It seems that the development of colorectal cancer is accompanied by complex changes in the metabolism of sphingolipids, causing not only qualitative shifts in the ceramide pool of cancer tissue but also quantitative disturbances, depending on the location of the primary tumor.
3
Albouery, Mayssa, Alexis Bretin, Bénédicte Buteau, Stéphane Grégoire, Lucy Martine, Ségolène Gambert, Alain M. Bron, Niyazi Acar, Benoit Chassaing, and Marie-Agnès Bringer. "Soluble Fiber Inulin Consumption Limits Alterations of the Gut Microbiota and Hepatic Fatty Acid Metabolism Caused by High-Fat Diet." Nutrients 13, no. 3 (March 23, 2021): 1037. http://dx.doi.org/10.3390/nu13031037.
Повний текст джерелаАнотація:
Diet shapes the gut microbiota which impacts hepatic lipid metabolism. Modifications in liver fat content are associated with metabolic disorders. We investigated the extent of dietary fat and fiber-induced alterations in the composition of gut microbiota and hepatic fatty acids (FAs). Mice were fed a purified low-fat diet (LFD) or high-fat diet (HFD) containing non-soluble fiber cellulose or soluble fiber inulin. HFD induced hepatic decreases in the amounts of C14:0, C16:1n-7, C18:1n-7 and increases in the amounts of C17:0, C20:0, C16:1n-9, C22:5n-3, C20:2n-6, C20:3n-6, and C22:4n-6. When incorporated in a LFD, inulin poorly affected the profile of FAs. However, when incorporated in a HFD, it (i) specifically led to an increase in the amounts of hepatic C18:0, C22:0, total polyunsaturated FAs (PUFAs), total n-6 PUFAs, C18:3n-3, and C18:2n-6, (ii) exacerbated the HFD-induced increase in the amount of C17:0, and (iii) prevented the HFD-induced increases in C16:1n-9 and C20:3n-6. Importantly, the expression/activity of some elongases and desaturases, as well as the gut microbiota composition, were impacted by the dietary fat and fiber content. To conclude, inulin modulated gut microbiota and hepatic fatty acid composition, and further investigations will determine whether a causal relationship exists between these two parameters.
4
Tobin, V., M. Le Gall, M. Romero, A. Leturque, and E. Brot-Laroche. "C26 - L’insuline est un inhibiteur de la localisation apicale de GLUT2 dans les entérocytes : une étude in vivo chez la souris et in vitro dans les cellules CACO-2/TC7." Gastroentérologie Clinique et Biologique 30, no. 1 (January 2006): 86. http://dx.doi.org/10.1016/s0399-8320(06)73108-6.
Повний текст джерела
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Detzner, Johanna, Charlotte Püttmann, Gottfried Pohlentz, Hans-Ulrich Humpf, Alexander Mellmann, Helge Karch, and Johannes Müthing. "Primary Human Colon Epithelial Cells (pHCoEpiCs) Do Express the Shiga Toxin (Stx) Receptor Glycosphingolipids Gb3Cer and Gb4Cer and Are Largely Refractory but Not Resistant towards Stx." International Journal of Molecular Sciences 22, no. 18 (September 16, 2021): 10002. http://dx.doi.org/10.3390/ijms221810002.
Повний текст джерелаАнотація:
Shiga toxin (Stx) is released by enterohemorrhagic Escherichia coli (EHEC) into the human intestinal lumen and transferred across the colon epithelium to the circulation. Stx-mediated damage of human kidney and brain endothelial cells and renal epithelial cells is a renowned feature, while the sensitivity of the human colon epithelium towards Stx and the decoration with the Stx receptor glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer, Galα1-4Galβ1-4Glcβ1-1Cer) and globotetraosylceramide (Gb4Cer, GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1Cer) is a matter of debate. Structural analysis of the globo-series GSLs of serum-free cultivated primary human colon epithelial cells (pHCoEpiCs) revealed Gb4Cer as the major neutral GSL with Cer (d18:1, C16:0), Cer (d18:1, C22:1/C22:0) and Cer (d18:1, C24:2/C24:1) accompanied by minor Gb3Cer with Cer (d18:1, C16:0) and Cer (d18:1, C24:1) as the dominant lipoforms. Gb3Cer and Gb4Cer co-distributed with cholesterol and sphingomyelin to detergent-resistant membranes (DRMs) used as microdomain analogs. Exposure to increasing Stx concentrations indicated only a slight cell-damaging effect at the highest toxin concentration of 1 µg/mL for Stx1a and Stx2a, whereas a significant effect was detected for Stx2e. Considerable Stx refractiveness of pHCoEpiCs that correlated with the rather low cellular content of the high-affinity Stx-receptor Gb3Cer renders the human colon epithelium questionable as a major target of Stx1a and Stx2a.
6
Detzner, Johanna, Elisabeth Krojnewski, Gottfried Pohlentz, Daniel Steil, Hans-Ulrich Humpf, Alexander Mellmann, Helge Karch, and Johannes Müthing. "Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity." Toxins 13, no. 2 (February 12, 2021): 139. http://dx.doi.org/10.3390/toxins13020139.
Повний текст джерелаАнотація:
Human kidney epithelial cells are supposed to be directly involved in the pathogenesis of the hemolytic–uremic syndrome (HUS) caused by Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC). The characterization of the major and minor Stx-binding glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), respectively, of primary human renal cortical epithelial cells (pHRCEpiCs) revealed GSLs with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Using detergent-resistant membranes (DRMs) and non-DRMs, Gb3Cer and Gb4Cer prevailed in the DRM fractions, suggesting their association with microdomains in the liquid-ordered membrane phase. A preference of Gb3Cer and Gb4Cer endowed with C24:0 fatty acid accompanied by minor monounsaturated C24:1-harboring counterparts was observed in DRMs, whereas the C24:1 fatty acid increased in relation to the saturated equivalents in non-DRMs. A shift of the dominant phospholipid phosphatidylcholine with saturated fatty acids in the DRM to unsaturated species in the non-DRM fractions correlated with the GSL distribution. Cytotoxicity assays gave a moderate susceptibility of pHRCEpiCs to the Stx1a and Stx2a subtypes when compared to highly sensitive Vero-B4 cells. The results indicate that presence of Stx-binding GSLs per se and preferred occurrence in microdomains do not necessarily lead to a high cellular susceptibility towards Stx.
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Guo, Tianyao, Zhigui Duan, Jia Chen, Chunliang Xie, Ying Wang, Ping Chen, and Xianchun Wang. "Pull-down combined with proteomic strategy reveals functional diversity of synaptotagmin I." PeerJ 5 (February 8, 2017): e2973. http://dx.doi.org/10.7717/peerj.2973.
Повний текст джерелаАнотація:
Synaptotagmin I (Syt I) is most abundant in the brain and is involved in multiple cellular processes. Its two C2 domains, C2A and C2B, are the main functional regions. Our present study employed a pull-down combined with proteomic strategy to identify the C2 domain-interacting proteins to comprehensively understand the biological roles of the C2 domains and thus the functional diversity of Syt I. A total of 135 non-redundant proteins interacting with the C2 domains of Syt I were identified. Out of them, 32 and 64 proteins only bound to C2A or C2B domains, respectively, and 39 proteins bound to both of them. Compared with C2A, C2B could bind to many more proteins particularly those involved in synaptic transmission and metabolic regulation. Functional analysis indicated that Syt I may exert impacts by interacting with other proteins on multiple cellular processes, including vesicular membrane trafficking, synaptic transmission, metabolic regulation, catalysis, transmembrane transport and structure formation, etc. These results demonstrate that the functional diversity of Syt I is higher than previously expected, that its two domains may mediate the same and different cellular processes cooperatively or independently, and that C2B domain may play even more important roles than C2A in the functioning of Syt I. This work not only further deepened our understanding of the functional diversity of Syt I and the functional differences between its two C2 domains, but also provided important clues for the further related researches.
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Detzner, Johanna, Anna-Lena Klein, Gottfried Pohlentz, Elisabeth Krojnewski, Hans-Ulrich Humpf, Alexander Mellmann, Helge Karch, and Johannes Müthing. "Primary Human Renal Proximal Tubular Epithelial Cells (pHRPTEpiCs): Shiga Toxin (Stx) Glycosphingolipid Receptors, Stx Susceptibility, and Interaction with Membrane Microdomains." Toxins 13, no. 8 (July 28, 2021): 529. http://dx.doi.org/10.3390/toxins13080529.
Повний текст джерелаАнотація:
Tubular epithelial cells of the human kidney are considered as targets of Shiga toxins (Stxs) in the Stx-mediated pathogenesis of hemolytic–uremic syndrome (HUS) caused by Stx-releasing enterohemorrhagic Escherichia coli (EHEC). Analysis of Stx-binding glycosphingolipids (GSLs) of primary human renal proximal tubular epithelial cells (pHRPTEpiCs) yielded globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer) with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Investigation of detergent-resistant membranes (DRMs) and nonDRMs, serving as equivalents for the liquid-ordered and liquid-disordered membrane phase, respectively, revealed the prevalence of Gb3Cer and Gb4Cer together with cholesterol and sphingomyelin in DRMs, suggesting lipid raft association. Stx1a and Stx2a exerted strong cellular damage with half-maximal cytotoxic doses (CD50) of 1.31 × 102 pg/mL and 1.66 × 103 pg/mL, respectively, indicating one order of magnitude higher cellular cytotoxicity of Stx1a. Surface acoustic wave (SAW) real-time interaction analysis using biosensor surfaces coated with DRM or nonDRM fractions gave stronger binding capability of Stx1a versus Stx2a that correlated with the lower cytotoxicity of Stx2a. Our study underlines the substantial role of proximal tubular epithelial cells of the human kidney being associated with the development of Stx-mediated HUS at least for Stx1a, while the impact of Stx2a remains somewhat ambiguous.
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Markowski, Adam R., Arkadiusz Żbikowski, Piotr Zabielski, Urszula Chlabicz, Patrycja Sadowska, Karolina Pogodzińska, and Agnieszka U. Błachnio-Zabielska. "The Effect of Silencing the Genes Responsible for the Level of Sphingosine-1-phosphate on the Apoptosis of Colon Cancer Cells." International Journal of Molecular Sciences 24, no. 8 (April 13, 2023): 7197. http://dx.doi.org/10.3390/ijms24087197.
Повний текст джерелаАнотація:
Sphingosine-1-phosphate (S1P) and ceramides (Cer) are engaged in key events of signal transduction, but their involvement in the pathogenesis of colorectal cancer is not conclusive. The aim of our study was to investigate how the modulation of sphingolipid metabolism through the silencing of the genes involved in the formation (SPHK1) and degradation (SGPL1) of sphingosine-1-phosphate would affect the sphingolipid profile and apoptosis of HCT-116 human colorectal cancer cells. Silencing of SPHK1 expression decreased S1P content in HCT-116 cells, which was accompanied by an elevation in sphingosine, C18:0-Cer, and C18:1-Cer, increase in the expression and activation of Caspase-3 and -9, and augmentation of apoptosis. Interestingly, silencing of SGLP1 expression increased cellular content of both the S1P and Cer (C16:0-; C18:0-; C18:1-; C20:0-; and C22:0-Cer), yet inhibited activation of Caspase-3 and upregulated protein expression of Cathepsin-D. The above findings suggest that modulation of the S1P level and S1P/Cer ratio regulates both cellular apoptosis and CRC metastasis through Cathepsin-D modulation. The cellular ratio of S1P/Cer seems to be a crucial component of the above mechanism.
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Al-Qarawi, AA, EF Abd Allah, and Hashem Abeer. "Effect of Ephedra alata Decne. on lipids metabolism of Aspergillus flavus Link." Bangladesh Journal of Botany 42, no. 1 (July 24, 2013): 45–50. http://dx.doi.org/10.3329/bjb.v42i1.15823.
Повний текст джерелаАнотація:
In Aspergillus flavus Link, the total lipid, sterols, neutral lipids, phospholipids and fatty acid content decreased significantly with the application of different concentrations of Ephedra alata Decne. extrtact. Gas chromatographic analysis revealed the presence of 12 fatty acids namely, (caprylic (C8), capric (C10), lauric (C12), myristic (C14), palmitic (C16), palmitoleic (C16:1), stearic (C18), oleic (C18:1), linoleic (C18:2), ? linolenic (C18:3), arachidic (C20) and arachidonic (C20:4) with total un-saturation per cent 69.78 in the cellular lipids of A. flavus. The use of E. alata extracts induced significant alteration in fatty acid profile towards increment saturation. DOI: http://dx.doi.org/10.3329/bjb.v42i1.15823 Bangladesh J. Bot. 42(1): 45-49, 2013 (June)
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Haribowo, A. Galih, J. Thomas Hannich, Agnès H. Michel, Márton Megyeri, Maya Schuldiner, Benoît Kornmann, and Howard Riezman. "Cytotoxicity of 1-deoxysphingolipid unraveled by genome-wide genetic screens and lipidomics in Saccharomyces cerevisiae." Molecular Biology of the Cell 30, no. 22 (October 15, 2019): 2814–26. http://dx.doi.org/10.1091/mbc.e19-07-0364.
Повний текст джерелаАнотація:
Hereditary sensory and autonomic neuropathy (HSAN) types IA and IC (IA/C) are caused by elevated levels of an atypical class of lipid named 1-deoxysphingolipid (DoxSL). How elevated levels of DoxSL perturb the physiology of the cell and how the perturbations lead to HSAN IA/C are largely unknown. In this study, we show that C26-1-deoxydihydroceramide (C26-DoxDHCer) is highly toxic to the cell, while C16- and C18-DoxDHCer are less toxic. Genome-wide genetic screens and lipidomics revealed the dynamics of DoxSL accumulation and DoxSL species responsible for the toxicity over the course of DoxSL accumulation. Moreover, we show that disruption of F-actin organization, alteration of mitochondrial shape, and accumulation of hydrophobic bodies by DoxSL are not sufficient to cause complete cellular failure. We found that cell death coincides with collapsed ER membrane, although we cannot rule out other possible causes of cell death. Thus, we have unraveled key principles of DoxSL cytotoxicity that may help to explain the clinical features of HSAN IA/C.
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Cantalupo, Anna, Linda Sasset, Antonella Gargiulo, Luisa Rubinelli, Ilaria Del Gaudio, Domenico Benvenuto, Christian Wadsack, Xiang-Chen Jiang, Maria Rosaria Bucci, and Annarita Di Lorenzo. "Endothelial Sphingolipid De Novo Synthesis Controls Blood Pressure by Regulating Signal Transduction and NO via Ceramide." Hypertension 75, no. 5 (May 2020): 1279–88. http://dx.doi.org/10.1161/hypertensionaha.119.14507.
Повний текст джерелаАнотація:
Ceramides are sphingolipids that modulate a variety of cellular processes via 2 major mechanisms: functioning as second messengers and regulating membrane biophysical properties, particularly lipid rafts, important signaling platforms. Altered sphingolipid levels have been implicated in many cardiovascular diseases, including hypertension, atherosclerosis, and diabetes mellitus–related conditions; however, molecular mechanisms by which ceramides impact endothelial functions remain poorly understood. In this regard, we generated mice defective of endothelial sphingolipid de novo biosynthesis by deleting the Sptlc2 (long chain subunit 2 of serine palmitoyltransferase)—the first enzyme of the pathway. Our study demonstrated that endothelial sphingolipid de novo production is necessary to regulate (1) signal transduction in response to NO agonists and, mainly via ceramides, (2) resting eNOS (endothelial NO synthase) phosphorylation, and (3) blood pressure homeostasis. Specifically, our findings suggest a prevailing role of C16:0-Cer in preserving vasodilation induced by tyrosine kinase and GPCRs (G-protein coupled receptors), except for Gq-coupled receptors, while C24:0- and C24:1-Cer control flow-induced vasodilation. Replenishing C16:0-Cer in vitro and in vivo reinstates endothelial cell signaling and vascular tone regulation. This study reveals an important role of locally produced ceramides, particularly C16:0-, C24:0-, and C24:1-Cer in vascular and blood pressure homeostasis, and establishes the endothelium as a key source of plasma ceramides. Clinically, specific plasma ceramides ratios are independent predictors of major cardiovascular events. Our data also suggest that plasma ceramides might be indicative of the diseased state of the endothelium.
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Robles-Heredia, Juan C., Alejandro Ruiz-Marín, Asteria Narváez-García, Luis E. Escalante-Montejo, Mario Martínez-De la Cruz, Yunuen Canedo-López, Luis J. Pérez-Reda, Francisco A. Tamayo-Ordóñez, and José del C. Zavala-Loría. "Study of the hydrodynamic effect in column PBR on cellular growth, nitrogen removal, lipid productivity and fatty acid profile in Chlorella vulgaris." Renewable Energy, Biomass & Sustainability 1, no. 1 (July 6, 2022): 33–44. http://dx.doi.org/10.56845/rebs.v1i1.11.
Повний текст джерелаАнотація:
In this work we analyzed different biochemical parameters such as cell growth, nitrogen removal, lipid productivity and fatty acid profile in Chlorella vulgaris by hydrodynamic effect varying the aeration to (0.75, 1.25, 1.75, 2.25) vvm and white light conditions continuous in column photobioreactor; hydrodynamic calculations of the FBR were carried out to determine the shear rate and possible existence of hydrodynamic stress at the proposed aeration conditions; the values reached in the shear rate were reduced (0.0025 to 0.0220) s-1, observing flow of homogeneous type in all the experiments; however, the maximum values of cell growth and specific growth rate (μ) were (5.90x106 cells mL-1 and 0.0229 d-1) respectively, as well as the highest N consumption (60%) and the highest productivity of lipids (8.98 mgL-1d-1) were reached during the experiment at 0.75 vvm. In relation to the analysis of the fatty acid profile greater presence of polyunsaturated fatty acids (PUFA) was observed in the experiments at 0.75 vvm, 1.75 vvm and 2.25 vvm, however, at 1.25 vvm, higher productivity of saturated fatty acids (SFA) was obtained; with respect to monounsaturated fatty acids (MUFA) the highest concentration was reflected at 0.75 vvm. The components with the highest presence in the fatty acid profile analysis were C12: 0; C20: 5N3; C24: 1; C 22: 0; C22: 2.
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Hakil, Florence, Oulfat Amin-Ali, Agnès Hirschler-Réa, Damien Mollex, Vincent Grossi, Robert Duran, Robert Matheron, and Cristiana Cravo-Laureau. "Desulfatiferula berrensis sp. nov., a n-alkene-degrading sulfate-reducing bacterium isolated from estuarine sediments." International Journal of Systematic and Evolutionary Microbiology 64, Pt_2 (February 1, 2014): 540–44. http://dx.doi.org/10.1099/ijs.0.057174-0.
Повний текст джерелаАнотація:
A novel sulfate-reducing bacterium designated strain BE2801T was isolated from oil-polluted estuarine sediments (Berre Lagoon, France). Cells were Gram-stain-negative, motile, slightly curved or vibrioid rods. Optimal growth of strain BE2801T occurred at 30–32 °C, 0.5–1.5% NaCl (w/v) and pH 7.2–7.4. Strain BE2801T grew with C4 to C20 fatty acids or C12 to C20 n-alkenes as electron donors. Acetate and carbon dioxide were the oxidation products. The major cellular fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7. The DNA G+C content was 50.2 mol%. 16S rRNA and dsrAB gene sequence analysis indicated that strain BE2801T was a member of the family Desulfobacteraceae within the class Deltaproteobacteria . DNA–DNA hybridization with the most closely related taxon demonstrated 14.8 % relatedness. Based on phenotypic and phylogenetic evidence, strain BE2801T ( = DSM 25524T = JCM 18157T) is proposed to be a representative of a novel species of the genus Desulfatiferula , for which the name Desulfatiferula berrensis sp. nov. is suggested.
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Sato, Naoki, Toru Yoshitomi, and Natsumi Mori-Moriyama. "Characterization and Biosynthesis of Lipids in Paulinella micropora MYN1: Evidence for Efficient Integration of Chromatophores into Cellular Lipid Metabolism." Plant and Cell Physiology 61, no. 5 (February 11, 2020): 869–81. http://dx.doi.org/10.1093/pcp/pcaa011.
Повний текст джерелаАнотація:
Abstract The chromatophores found in the cells of photosynthetic Paulinella species, once believed to be endosymbiotic cyanobacteria, are photosynthetic organelles that are distinct from chloroplasts. The chromatophore genome is similar to the genomes of α-cyanobacteria and encodes about 1,000 genes. Therefore, the chromatophore is an intriguing model of organelle formation. In this study, we analyzed the lipids of Paulinella micropora MYN1 to verify that this organism is a composite of cyanobacterial descendants and a heterotrophic protist. We detected glycolipids and phospholipids, as well as a betaine lipid diacylglyceryl-3-O-carboxyhydroxymethylcholine, previously detected in many marine algae. Cholesterol was the only sterol component detected, suggesting that the host cell is similar to animal cells. The glycolipids, presumably present in the chromatophores, contained mainly C16 fatty acids, whereas other classes of lipids, presumably present in the other compartments, were abundant in C20 and C22 polyunsaturated fatty acids. This suggests that chromatophores are metabolically distinct from the rest of the cell. Metabolic studies using isotopically labeled substrates showed that different fatty acids are synthesized in the chromatophore and the cytosol, which is consistent with the presence of both type I and type II fatty acid synthases, supposedly present in the cytosol and the chromatophore, respectively. Nevertheless, rapid labeling of the fatty acids in triacylglycerol and phosphatidylcholine by photosynthetically fixed carbon suggested that the chromatophores efficiently provide metabolites to the host. The metabolic and ultrastructural evidence suggests that chromatophores are tightly integrated into the whole cellular metabolism.
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Kurz, Jennifer, Robert Brunkhorst, Christian Foerch, Leonard Blum, Marina Henke, Laureen Gabriel, Thomas Ulshöfer, et al. "The relevance of ceramides and their synthesizing enzymes for multiple sclerosis." Clinical Science 132, no. 17 (September 14, 2018): 1963–76. http://dx.doi.org/10.1042/cs20180506.
Повний текст джерелаАнотація:
Ceramide synthases (CerS) synthesize chain length specific ceramides (Cer), which mediate cellular processes in a chain length-dependent manner. In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), we observed that the genetic deletion of CerS2 suppresses EAE pathology by interaction with granulocyte-colony stimulating factor (G-CSF) signaling and CXC motif chemokine receptor 2 (CXCR2) expression, leading to impaired neutrophil migration. In the present study, we investigated the importance of Cers and their synthesizing/metabolizing enzymes in MS. For this purpose, a longitudinal study with 72 MS patients and 25 healthy volunteers was performed. Blood samples were collected from healthy controls and MS patients over 1- or 3-year periods, respectively. Immune cells were counted using flow cytometry, ceramide levels were determined using liquid chromatography-tandem mass spectrometry, and mRNA expression was analyzed using quantitative PCR. In white blood cells, C16-LacCer and C24-Cer were down-regulated in MS patients in comparison with healthy controls. In plasma, C16-Cer, C24:1-Cer, C16-GluCer, and C24:1-GluCer were up-regulated and C16-LacCer was down-regulated in MS patients in comparison with healthy controls. Blood samples from MS patients were characterized by an increased B-cell number. However, there was no correlation between B-cell number and Cer levels. mRNA expression of Cer metabolizing enzymes and G-CSF signaling enzymes was significantly increased in MS patients. Interestingly, G-CSF receptor (G-CSFR) and CXCR2 mRNA expression correlated with CerS2 and UDP-glucose Cer glucosyltransferase (UGCG) mRNA expression. In conclusion, our results indicate that Cer metabolism is linked to G-CSF signaling in MS.
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Smakman, Niels, Diana J. M. van den Wollenberg, Inne H. M. Borel Rinkes, Rob C. Hoeben, and Onno Kranenburg. "Sensitization to Apoptosis Underlies KrasD12-Dependent Oncolysis of Murine C26 Colorectal Carcinoma Cells by Reovirus T3D." Journal of Virology 79, no. 23 (December 15, 2005): 14981–85. http://dx.doi.org/10.1128/jvi.79.23.14981-14985.2005.
Повний текст джерелаАнотація:
ABSTRACT Reovirus T3D is an oncolytic agent that preferentially targets tumor cells expressing an activated Ras oncogene. Ras signaling interferes with the cellular stress response that inhibits translation of reovirus RNAs. Murine C26 colorectal carcinoma cells express a mutant KrasD12 gene. Reovirus T3D efficiently kills C26 cells, but not C26 cells in which the KrasD12 mRNA is stably repressed by expression of KrasD12-directed short-hairpin RNAs. Surprisingly, neither reovirus T3D protein synthesis nor T3D virus yields were suppressed by deletion of KrasD12. Rather, reovirus-induced tumor cell apoptosis was completely abrogated as a result of Kras knockdown. We conclude that sensitization of C26 tumor cells to reovirus-induced apoptosis underlies the Ras dependency of reovirus T3D oncolysis.
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Nichols, David S., Peter D. Nichols, and Cornelius W. Sullivan. "Fatty acid, sterol and hydrocarbon composition of Antarctic sea ice diatom communities during the spring bloom in McMurdo Sound." Antarctic Science 5, no. 3 (September 1993): 271–78. http://dx.doi.org/10.1017/s0954102093000367.
Повний текст джерелаАнотація:
The lipid composition of microalgal communities dominated by diatoms collected from the sea ice at three locations within McMurdo Sound during the austral spring bloom of 1989/90, was determined using gas chromatography (GC) and GC mass spectrometry. A range of C27-C29 sterols were detected. The major sterols found at the three sites were 24-methylcholesta-5, 22E-diene-3β-ol (Cape Armitage); trans-22-dehydrocholesterol, 24-ethylcholesterol and 24-methylenecholesta (Erebus Ice Tongue); and 24-methylenecholesterol (Cape Royds). The difference in sterol profiles is believed to reflect the differing species composition at each site. The high relative levels (as % of total) of 24-ethylcholesterol at the Erebus Ice Tongue site (possibly related to Amphiprora kufferathii) supports the proposal that diatoms are a more probable source of C29 sterols in Antarctic lakes than are other algal groups or cyanobacteria. Changes in sterol composition over the course of the bloom were evident at the Cape Armitage site, particularly within the cellular free-lipid fraction. The major fatty acids identified were 14:0, 16:0, 16:1ω7c, 16:4ω1 and 20:5ω3 (Cape Armitage and Erebus Ice tongue sites); 16:0, 16:1ω7c and 20:5ω3 (Cape Royds site). All sites demonstrated high levels of PUFA (40–50% of total fatty acids), with an average 20:5ω3 level of 21% Erebus Ice Tongue, 20% Cape Royds, and 17% Cape Armitage. Variation was also observed in the percentage of 20:5ω3 for the Cape Armitage community over the sampling period. Levels of 22:6ω3 were between 0.4 and 1% of total fatty acids for the three sites. A C25:2 isoprenoid hydrocarbon was present in samples from all sites, adding further evidence to the proposal that diatoms are probably a source of this and related isoprenoid alkenes in marine and coastal sediments.
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Spassieva, Stefka D., Thomas D. Mullen, Danyelle M. Townsend, and Lina M. Obeid. "Disruption of ceramide synthesis by CerS2 down-regulation leads to autophagy and the unfolded protein response." Biochemical Journal 424, no. 2 (November 11, 2009): 273–83. http://dx.doi.org/10.1042/bj20090699.
Повний текст джерелаАнотація:
Ceramide metabolism has come under recent scrutiny because of its role in cellular stress responses. CerS2 (ceramide synthase 2) is one of the six mammalian isoforms of ceramide synthase and is responsible for the synthesis of VLC (very-long-chain) ceramides, e.g. C24, C24:1. To study the role of CerS2 in ceramide metabolism and cellular homoeostasis, we down-regulated CerS2 using siRNA (small interfering RNA) and examined several aspects of sphingolipid metabolism and cell stress responses. CerS2 down-regulation had a broad effect on ceramide homoeostasis, not just on VLC ceramides. Surprisingly, CerS2 down-regulation resulted in significantly increased LC (long-chain) ceramides, e.g. C14, C16, and our results suggested that the increase was due to a ceramide synthase-independent mechanism. CerS2-down-regulation-induced LC ceramide accumulation resulted in growth arrest which was not accompanied by apoptotic cell death. Instead, cells remained viable, showing induction of autophagy and activation of PERK [PKR (double-stranded-RNA-dependent protein kinase)-like endoplasmic reticulum kinase] and IRE1 (inositol-requiring 1) pathways [the latter indicating activation of the UPR (unfolded protein response)].
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Kang, J. X., S. F. P. Man, A. J. Hirsh, and M. T. Clandinin. "Characterization of platelet-activating factor binding to human airway epithelial cells: modulation by fatty acids and ion-channel blockers." Biochemical Journal 303, no. 3 (November 1, 1994): 795–802. http://dx.doi.org/10.1042/bj3030795.
Повний текст джерелаАнотація:
Radioligand-binding studies were performed in primary cultured human airway epithelial cells with [3H]PAF to determine whether these cells express platelet-activating factor (PAF) receptors. Scatchard analysis of PAF binding data revealed a single class of PAF binding sites with Kd 1.8 +/- 0.2 nM and Bmax. 21.0 +/- 2.1 fmol/10(6) cells (13,000 receptors/cell). PAF binding increased the intracellular free Ca2+ concentration ([Ca2+]i), indicating functional PAF receptors. Palmitate (C16:0), linoleic acid (C18:2 omega 6) or eicosapentaenoic acid (C20:5 omega 3) was incubated with the cells to test the effect on PAF binding. Incorporation of each fatty acid into cellular phospholipid occurred. [3H]PAF (1 nM) binding decreased in cells supplemented with C20:5 omega 3, but increased in the cells supplemented with C16:0. Scatchard analysis revealed that the inhibition of PAF binding by supplementation with C20:5 omega 3 was due to a decrease in both affinity and number of PAF receptors. PAF-stimulated increase in [Ca2+]i was also decreased by 60% in cells supplemented with C20:5 omega 3. Verapamil, a Ca(2+)-channel blocker, and amiloride, a Na(+)-channel blocker, inhibited specific binding of [3H]PAF to the cells, with IC50 4-5 microM and 0.2 mM respectively. Diphenylamine-2-carboxylate (DPC), a Cl(-)-channel blocker, dramatically increased PAF binding to the cell in a dose-dependent manner. Scatchard analysis revealed that verapamil and amiloride decreased both binding affinity and number of PAF receptors, whereas DPC increased PAF binding sites without affecting binding affinity. These results demonstrate that human airway epithelial cells have a functional receptor for PAF and that PAF receptor binding can be modulated by exogenous fatty acids and by ion-channel blockers.
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Nury, Thomas, Margaux Doria, Gérard Lizard, and Anne Vejux. "Docosahexaenoic Acid Attenuates Mitochondrial Alterations and Oxidative Stress Leading to Cell Death Induced by Very Long-Chain Fatty Acids in a Mouse Oligodendrocyte Model." International Journal of Molecular Sciences 21, no. 2 (January 18, 2020): 641. http://dx.doi.org/10.3390/ijms21020641.
Повний текст джерелаАнотація:
In the case of neurodegenerative pathologies, the therapeutic arsenal available is often directed towards the consequences of the disease. The purpose of this study is, therefore, to evaluate the ability of docosahexaenoic acid (DHA), a molecule present in certain foods and considered to have health benefits, to inhibit the cytotoxic effects of very long-chain fatty acids (C24:0, C26:0), which can contribute to the development of some neurodegenerative diseases. The effect of DHA (50 µM) on very long-chain fatty acid-induced toxicity was studied by several complementary methods: phase contrast microscopy to evaluate cell viability and morphology, the MTT test to monitor the impact on mitochondrial function, propidium iodide staining to study plasma membrane integrity, and DHE staining to measure oxidative stress. A Western blot assay was used to assess autophagy through modification of LC3 protein. The various experiments were carried out on the cellular model of 158N murine oligodendrocytes. In 158N cells, our data establish that DHA is able to inhibit all tested cytotoxic effects induced by very long-chain fatty acids.
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Cowart, L. Ashley, Yasuo Okamoto, Xinghua Lu, and Yusuf A. Hannun. "Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae." Biochemical Journal 393, no. 3 (January 13, 2006): 733–40. http://dx.doi.org/10.1042/bj20050643.
Повний текст джерелаАнотація:
Saccharomyces cerevisiae produces the sphingolipid ceramide by de novo synthesis as well as by hydrolysis of complex sphingolipids by Isc1p (inositolphosphoceramide-phospholipase C), which is homologous with the mammalian neutral sphingomyelinases. Though the roles of sphingolipids in yeast stress responses are well characterized, it has been unclear whether Isc1p contributes to stress-induced sphingolipids. The present study was undertaken in order to distinguish the relative roles of de novo sphingolipid biosynthesis versus Isc1p-mediated sphingolipid production in the heat-stress response. Ceramide production was measured at normal and increased temperature in an ISC1 deletion and its parental strain (ISC1 being the gene that codes for Isc1p). The results showed that Isc1p contributes specifically to the formation of the C24-, C24:1- and C26-dihydroceramide species. The interaction between these two pathways of sphingolipid production was confirmed by the finding that ISC1 deletion is synthetically lethal with the lcb1-100 mutation. Interestingly, Isc1p did not contribute significantly to transient cell-cycle arrest or growth at elevated temperature, responses known to be regulated by the de novo pathway. In order to define specific contributions of ISC1, microarray hybridizations were performed, and analyses showed misregulation of genes involved in carbon source utilization and sexual reproduction, which was corroborated by defining a sporulation defect of the isc1Δ strain. These results indicate that the two pathways of ceramide production in yeast interact, but differ in their regulation of ceramides of distinct molecular species and serve distinct cellular functions.
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Xie, Zhangzhang, Weitie Lin, and Jianfei Luo. "Comparative Phenotype and Genome Analysis ofCellvibriosp. PR1, a Xylanolytic and Agarolytic Bacterium from the Pearl River." BioMed Research International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/6304248.
Повний текст джерелаАнотація:
Cellvibriosp. PR1 is a xylanolytic and agarolytic bacterium isolated from the Pearl River. Strain PR1 is closely related toCellvibrio fibrivoransandC. ostraviensis(identity > 98%). The xylanase and agarase contents of strain PR1 reach up to 15.4 and 25.9 U/mL, respectively. The major cellular fatty acids consisted of C16:0 (36.7%), C18:0 (8.8%), C20:0 (6.8%), C15:0iso 2-OH or/and C16:1ω7c (17.4%), and C18:1ω7c or/and C18:1ω6c (6.7%). A total of 251 CAZyme modules (63 CBMs, 20 CEs, 128 GHs, 38 GTs, and 2 PLs) were identified from 3,730 predicted proteins. Genomic analysis suggested that strain PR1 has a complete xylan-hydrolyzing (5β-xylanases, 16β-xylosidases, 17α-arabinofuranosidases, 9 acetyl xylan esterases, 4α-glucuronidases, and 2 ferulic acid esterases) and agar-hydrolyzing enzyme system (2β-agarases and 2α-neoagarooligosaccharide hydrolases). In addition, the main metabolic pathways of xylose, arabinose, and galactose are established in the genome-wide analysis. This study shows that strain PR1 contains a large number of glycoside hydrolases.
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Ali, Omeralfaroug, Miklós Mézes, Krisztián Balogh, Melinda Kovács, and András Szabó. "The Effects of Mixed Fusarium Mycotoxins at EU-Permitted Feed Levels on Weaned Piglets’ Tissue Lipids." Toxins 13, no. 7 (June 27, 2021): 444. http://dx.doi.org/10.3390/toxins13070444.
Повний текст джерелаАнотація:
At exactly the individual permitted EU-tolerance dietary limits, fumonisins (FB: 5 mg/kg diet) and mixed fusariotoxins (DZ: 0.9 mg deoxynivalenol + 0.1 mg zearalenone/kg diet, and FDZ: 5 mg fumonisins + 0.9 mg deoxynivalenol + 0.1 mg zearalenone/kg diet) were administered to piglets (n = 6/group) for three weeks. Bodyweights of intoxicated piglets increased, while feed conversion ratios decreased. In FDZ, both the absolute and relative weight of the liver decreased. In the renal-cellular membrane, the most pronounced alterations were in FDZ treatment, followed by individual FB exposure. In both treatments, high proportions of C20:0 and C22:0 with low fatty acid (FA) unsaturation were found. In hepatocyte phospholipids, FDZ toxins exerted antagonistic interactions, and FB had the strongest increasing effect on FA monounsaturation. Among all investigated organs, the spleen lipids were the least responsive, in which FDZ expressed synergistic reactions on C20:0 (↑ FDZ vs. FB) and C22:0 (↓ FDZ vs. DZ). The antioxidant defense of the kidney was depleted (↓ glutathione concentration by FB-exposure). Blood plasma indicated renal injury (profound increase of urea and creatinine in FB vs. DZ and FDZ). FB strongly increased total-cholesterol and low density lipoprotein concentrations, whereas FDZ synergistically increased gamma-glutamyltransferase, alkaline-phosphatase, calcium and phosphorus levels. Summarized, individual and combined multiple fusariotoxins modified the membrane lipid profile and antioxidant defense of splanchnic organs, and serum biochemicals, without retarding growth in piglets.
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Satpati, Gour Gopal, Sanjit Kanjilal, Rachapudi Badari Narayana Prasad, and Ruma Pal. "Rapid Accumulation of Total Lipid inRhizoclonium africanumKutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level." International Journal of Microbiology 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/275035.
Повний текст джерелаАнотація:
Increase of total lipid and the proportion of the favorable fatty acids in marine green filamentous macroalgaRhizoclonium africanum(Chlorophyceae) was studied under nitrate and phosphate limitations. These stresses were given by both eliminating and doubling the required amounts of nitrate and phosphate salts in the growth media. A significant twofold increase in total lipid (193.03 mg/g) was achieved in cells in absence of nitrate in the culture medium, followed by phosphate limitation (142.65 mg/g). The intracellular accumulation of neutral lipids was observed by fluorescence microscopy. The scanning electron microscopic study showed the major structural changes under nutrient starvation. Fourier transform infrared spectroscopy (FTIR) revealed the presence of ester (C-O-C stretching), ketone (C-C stretching), carboxylic acid (O-H bending), phosphine (P-H stretching), aromatic (C-H stretching and bending), and alcohol (O-H stretching and bending) groups in the treated cells indicating the high accumulation of lipid hydrocarbons in the treated cells. Elevated levels of fatty acids favorable for biodiesel production, that is, C16:0, C16:1, C18:1, and C20:1, were identified under nitrate- and phosphate-deficient conditions. This study shows that the manipulation of cultural conditions could affect the biosynthetic pathways leading to increased lipid production while increasing the proportion of fatty acids suitable for biodiesel production.
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Mizutani, Yukiko, Akio Kihara, and Yasuyuki Igarashi. "Mammalian Lass6 and its related family members regulate synthesis of specific ceramides." Biochemical Journal 390, no. 1 (August 9, 2005): 263–71. http://dx.doi.org/10.1042/bj20050291.
Повний текст джерелаАнотація:
The Lass (longevity-assurance homologue) family members, which are highly conserved among eukaryotes, function in ceramide synthesis. In the mouse, there are at least five Lass family members, Lass1, Lass2, Lass4, Lass5 and the hitherto uncharacterized Lass6. To investigate specific roles for each Lass member in ceramide synthesis, we cloned these five mouse proteins. Overproduction of any Lass protein in cultured cells resulted in an increase in cellular ceramide, but the ceramide species produced varied. Overproduction of Lass1 increased C18:0-ceramide levels preferentially, and overproduction of Lass2 and Lass4 increased levels of longer ceramides such as C22:0- and C24:0-ceramides. Lass5 and Lass6 produced shorter ceramide species (C14:0- and C16:0-ceramides); however, their substrate preferences towards saturated/unsaturated fatty acyl-CoA differed. In addition to differences in substrate preferences, we also demonstrated by Northern blotting that Lass family members are differentially expressed among tissues. Additionally, we found that Lass proteins differ with regard to glycosylation. Of the five members, only Lass2, Lass5 and Lass6 were N-glycosylated, each at their N-terminal Asn residue. The occurrence of N-glycosylation of some Lass proteins provides topological insight, indicating that the N-termini of Lass family members probably face the luminal side of the endoplasmic reticulum membrane. Furthermore, based on a proteinase K digestion assay, we demonstrated that the C-terminus of Lass6 faces the cytosolic side of the membrane. From these data we propose topology for the conserved Lag1 motif in Lass family members, namely that the N-terminal region faces the luminal side and the C-terminal region the cytosolic side of the endoplasmic reticulum membrane.
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Baik, Keun Sik, Jong-Soon Choi, Joseph Kwon, Seong Chan Park, Yeoung Min Hwang, Mi Sun Kim, Eun Mi Kim, Dong-Cheol Seo, Ju-Sik Cho, and Chi Nam Seong. "Terriglobus aquaticus sp. nov., isolated from an artificial reservoir." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (December 1, 2013): 4744–49. http://dx.doi.org/10.1099/ijs.0.050724-0.
Повний текст джерелаАнотація:
A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4T, was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03′ 43′′ N 127° 14′ 15′′ E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4T grew at pH 6–7 (optimum, pH 6) and at 15–30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Terriglobus , showing sequence similarities of 97.09 % and 96.82 % to Terriglobus roseus DSM 18391T and Terriglobus saanensis SP1PR4T, respectively. Low rpoB gene sequence similarity with members of the genus Terriglobus and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus Terriglobus . The major cellular fatty acids were iso-C15 : 0, C16 : 0, C20 : 1ω9c, C14 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The DNA G+C content of strain 03SUJ4T was 63.2±0.1 mol% (mean±sd of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus Terriglobus . On the basis of the evidence presented in this study, a novel species, Terriglobus aquaticus sp. nov. is proposed for strain 03SUJ4T ( = KCTC 23332T = JCM 17517T).
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Uskokovic, Milan R., George P. Studzinski, Jeffrey P. Gardner, Satyanarayana G. Reddy, Moray J. Campbell, and H. Philip Koeffler. "The 16-Ene Vitamin D Analogs." Current Pharmaceutical Design 3, no. 1 (February 1997): 99–123. http://dx.doi.org/10.2174/138161280301221006095220.
Повний текст джерелаАнотація:
Numerous 16-ene vitamin D analogs were investigated as potential anticancer agents. Several structural modifications have been uncovered that contribute to the improvement in the stimulation of HL-60 cells differentiation, the inhibition of HL-60 cells proliferation and the reduction of calcemic properties in vivo. They include the introduction of 16-, 22E-, 23E- and 23Z-double bonds, 23-triple bond or 22R-allene, and substitution of C26 and C27-hydrogens with fluorine or methyl groups. The biggest gains have been achieved by combination of the 16-double bond with 23-double or triple bond and 26-trifluoro or 26,27-hexafluoro substitution patterns. Separately, the combination of the 16-double bond with 22R-allene has produced a highly active analog. In respect to modifications in the ring A, the high activities in cell differentiation and inhibition of cell proliferation with significant reduction of calcemic properties were observed in the lα-fluoro, 3-desoxy, and 19-nor series. It was also shown that the lack of the lα-hydroxy group can be overcome by an optimized modification in the ring D and the side chain; 25(OH)-16,23E-diene-26,27- F6D3 is fully active in HL-60 cell differentiation assay with only mimimal effects on the cellular calcium homeostasis.
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Ammah, Adolf, Duy Do, Nathalie Bissonnette, Nicolas Gévry, and Eveline Ibeagha-Awemu. "Co-Expression Network Analysis Identifies miRNA–mRNA Networks Potentially Regulating Milk Traits and Blood Metabolites." International Journal of Molecular Sciences 19, no. 9 (August 24, 2018): 2500. http://dx.doi.org/10.3390/ijms19092500.
Повний текст джерелаАнотація:
MicroRNAs (miRNA) regulate mRNA networks to coordinate cellular functions. In this study, we constructed gene co-expression networks to detect miRNA modules (clusters of miRNAs with similar expression patterns) and miRNA–mRNA pairs associated with blood (triacylglyceride and nonesterified fatty acids) and milk (milk yield, fat, protein, and lactose) components and milk fatty acid traits following dietary supplementation of cows’ diets with 5% linseed oil (LSO) (n = 6 cows) or 5% safflower oil (SFO) (n = 6 cows) for 28 days. Using miRNA transcriptome data from mammary tissues of cows for co-expression network analysis, we identified three consensus modules: blue, brown, and turquoise, composed of 70, 34, and 86 miRNA members, respectively. The hub miRNAs (miRNAs with the most connections with other miRNAs) were miR-30d, miR-484 and miR-16b for blue, brown, and turquoise modules, respectively. Cell cycle arrest, and p53 signaling and transforming growth factor–beta (TGF-β) signaling pathways were the common gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched for target genes of the three modules. Protein percent (p = 0.03) correlated with the turquoise module in LSO treatment while protein yield (p = 0.003) and milk yield (p = 7 × 10−04) correlated with the turquoise model, protein and milk yields and lactose percent (p < 0.05) correlated with the blue module and fat percent (p = 0.04) correlated with the brown module in SFO treatment. Several fatty acids correlated (p < 0.05) with the blue (CLA:9,11) and brown (C4:0, C12:0, C22:0, C18:1n9c and CLA:10,12) modules in LSO treatment and with the turquoise (C14:0, C18:3n3 and CLA:9,11), blue (C14:0 and C23:0) and brown (C6:0, C16:0, C22:0, C22:6n3 and CLA:10,12) modules in SFO treatment. Correlation of miRNA and mRNA data from the same animals identified the following miRNA–mRNA pairs: miR-183/RHBDD2 (p = 0.003), miR-484/EIF1AD (p = 0.011) and miR-130a/SBSPON (p = 0.004) with lowest p-values for the blue, brown, and turquoise modules, respectively. Milk yield, protein yield, and protein percentage correlated (p < 0.05) with 28, 31 and 5 miRNA–mRNA pairs, respectively. Our results suggest that, the blue, brown, and turquoise modules miRNAs, hub miRNAs, miRNA–mRNA networks, cell cycle arrest GO term, p53 signaling and TGF-β signaling pathways have considerable influence on milk and blood phenotypes following dietary supplementation of dairy cows’ diets with 5% LSO or 5% SFO.
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Bunnoy, Na-Nakorn, Kayansamruaj, and Srisapoome. "Acinetobacter Strain KUO11TH, a Unique Organism Related to Acinetobacter pittii and Isolated from the Skin Mucus of Healthy Bighead Catfish and Its Efficacy Against Several Fish Pathogens." Microorganisms 7, no. 11 (November 10, 2019): 549. http://dx.doi.org/10.3390/microorganisms7110549.
Повний текст джерелаАнотація:
The bacterial strain KU011TH was isolated from the skin mucus of healthy bighead catfish. The strain is a Gram-negative coccobacillus that is nonmotile, aerobic, catalase positive, oxidase negative, and nonhemolytic. Sequence analyses of the housekeeping genes 16S rRNA, gyrB and rpoB indicate that this strain is a new member of the Acb complex of the genus Acinetobacter and is closely related to Acinetobacter pittii and Acinetobacter lactucae. In addition, the genome relatedness-associated ANIb (<95–96%) and in silico DDH (<70%) values clearly supported the new member of the genus Acinetobacter and the Acb complex. The genome of the strain KU011TH was approximately 3.79 Mbp in size, comprising 3619 predicted genes, and the DNA G+C content was 38.56 mol%. The major cellular fatty acids were C18:1ω9c, C16:0, C16:1, C20:2, C18:2ω6c and C18:1ω9t. The whole-genome sequences and phenotypic, phylogenetic, and chemotaxonomic data clearly support the classification of the strain KU011TH as a new member in the genus Acinetobacter which is closest to A. pittii. Additionally, the new bacterial strain exhibited strong activity against a broad range of freshwater fish pathogens in vitro.
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Zager, R. A., D. S. Conrad, and K. Burkhart. "Phospholipase A2: a potentially important determinant of adenosine triphosphate levels during hypoxic-reoxygenation tubular injury." Journal of the American Society of Nephrology 7, no. 11 (November 1996): 2327–39. http://dx.doi.org/10.1681/asn.v7112327.
Повний текст джерелаАнотація:
During the course of O2 deprivation-induced proximal tubular injury, profound alterations in ATP homeostasis exist. This study sought to characterize direct cellular determinants of these abnormalities further. Mouse proximal tubular segments (PTS) were isolated and their adenine nucleotide profiles were determined during hypoxic-reoxygenation injury. The extent of oxidant stress, Ca2+ overload, cytoskeletal disruption, and phospholipase activity were experimentally manipulated by H2O2, Ca2+ ionophore, cytochalasin D, or PLA2 addition, respectively. Hypoxia induced the expected deterioration in adenylate profiles, and a persistent defect in ATP homeostasis was observed during reoxygenation (decreased ATP/ADP ratios and absolute ATP content). H2O2, Ca2+ ionophore, and cytochalasin D had no significant impact on adenylate profiles. However, doses of PLA2 that had no overt effect on normal tubules caused 50 to 75% reductions in both hypoxic and reoxygenation ATP/ADP ratios and absolute ATP content. This effect was completely reproduced by the addition of arachidonic acid (C20:4). No other test fatty acid (C16:0, C18:1, C18:3) reproduced this result. Despite its profound negative impact on hypoxic/reoxygenation ATP concentrations, PLA2 and C20:4 each decreased lethal cell injury (lactate dehydrogenase release), as previously reported. The reductions in ATP and lethal cell injury were not mechanistically linked, because C18:1 and C18:3 reproduced the protective action of C20:4 without altering adenine nucleotide profiles. Ouabain, mannitol, or plasma membrane fatty acid "scavenger" therapy (albumin) did not improve the posthypoxic/PLA2-induced depressions in ATP. The addition of C20:4 caused a modest decrease in posthypoxic tubule oxygen consumption, compared to controls. It was concluded that: (1) PLA2 can be a major determinant of ATP concentrations during both hypoxic and reoxygenation tubular injury; (2) this action is mediated via C20:4 release; (3) a primary defect in mitochondrial ATP production, rather than increased ATP consumption, is likely to be responsible for this action.
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Valenzuela, Rodrigo, Macarena Ortiz, María Catalina Hernández-Rodas, Francisca Echeverría, and Luis Alberto Videla. "Targeting n-3 Polyunsaturated Fatty Acids in Non-Alcoholic Fatty Liver Disease." Current Medicinal Chemistry 27, no. 31 (September 11, 2020): 5250–72. http://dx.doi.org/10.2174/0929867326666190410121716.
Повний текст джерелаАнотація:
Background: Non-Alcoholic Fatty Liver Disease (NAFLD) is characterized by abnormal hepatic accumulation of triacylglycerides in the absence of alcohol consumption, in association with Oxidative Stress (OS), a pro-inflammatory state and Insulin Resistance (IR), which are attenuated by n-3 long-chain polyunsaturated Fatty Acids (FAs) C20-C22 (LCPUFAs) supplementation. Main causes of NAFLD comprise high caloric intake and a sedentary lifestyle, with high intakes of saturated FAs. Methods: The review includes several searches considering the effects of n-3 LCPUFAs in NAFLD in vivo and in vitro models, using the PubMed database from the National Library of Medicine- National Institutes of Health. Results: The LCPUFAs eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n- 3, DHA) have a positive effect in diminishing liver steatosis, OS, and the levels of aspartate aminotransferase, alanine aminotransferase and pro-inflammatory cytokines, with improvement of insulin sensitivity and adiponectin levels. The molecular pathways described for n-3 LCPUFAs in cellular and animal models and humans include peroxisome proliferator–activated receptor-α activation favouring FA oxidation, diminution of lipogenesis due to sterol responsive element binding protein-1c downregulation and inflammation resolution. Besides, nuclear factor erythroid-2-related factor-2 activation is elicited by n-3 LCPUFA-derived oxidation products producing direct and indirect antioxidant responses, with concomitant anti-fibrogenic action. Conclusion: The discussed effects of n-3 LCPUFA supplementation support its use in NAFLD, although having a limited value in NASH, a contention that may involve n-3 LCPUFA oxygenated derivatives. Clinical trials establishing optimal dosages, intervention times, type of patients and possible synergies with other natural products are needed in future studies.
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Park, Seong Chan, Yeoung Min Hwang, Ji Hee Lee, Keun Sik Baik, and Chi Nam Seong. "Algibacter agarivorans sp. nov. and Algibacter agarilyticus sp. nov., isolated from seawater, reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. and emended description of the genus Algibacter." International Journal of Systematic and Evolutionary Microbiology 63, Pt_9 (September 1, 2013): 3494–500. http://dx.doi.org/10.1099/ijs.0.051300-0.
Повний текст джерелаАнотація:
Two yellow-pigmented, rod-shaped, non-motile, Gram-reaction-negative and aerobic bacterial strains, designated KYW560T and KYW563T, were isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolates required sea salts for growth. Flexirubin-type pigments were absent. The common major cellular fatty acids (>5 % of total) of the two strains were C16 : 0, C18 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Strain KYW560T also contained iso-C15 : 0 3-OH and C20 : 1ω9c as major fatty acids. The main polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The predominant isoprenoid quinone was MK-6. The DNA G+C contents of strains KYW560T and KYW563T were 41.0±0.7 and 38.3±0.4 mol% (mean±sd of three determinations), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates belonged to the family Flavobacteriaceae , and were related to the genus Algibacter . Based on data from this taxonomic study using a polyphasic approach, it is proposed that the isolates represent novel species of the genus Algibacter , for which the names Algibacter agarivorans sp. nov. (type strain, KYW560T = KCTC 23855T = JCM 18285T) and Algibacter agarilyticus sp. nov. (type strain, KYW563T = KCTC 23857T = JCM 18275T) are proposed. Reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. and emended description of the genus Algibacter are also proposed.
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Belharbi, R., H. Devaud, M. E. Forgue-Lafitte, A. Tsocas, P. Plaisancié, C. Sapin, M. Lombes, C. Laboisse, A. Bado та J. C. Marie. "C29 - FIZZ-2/RELM-ɛ stimule la sécrétion de mucine des cellules intestinales". Gastroentérologie Clinique et Biologique 30, № 1 (січень 2006): 88. http://dx.doi.org/10.1016/s0399-8320(06)73111-6.
Повний текст джерела
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Storck, Wiebke, Iris Meisen, Kathrin Gianmoena, Ina Pläger, Ivan U. Kouzel, Martina Bielaszewska, Jörg Haier, et al. "Shiga toxin glycosphingolipid receptor expression and toxin susceptibility of human pancreatic ductal adenocarcinomas of differing origin and differentiation." Biological Chemistry 393, no. 8 (August 1, 2012): 785–99. http://dx.doi.org/10.1515/hsz-2012-0165.
Повний текст джерелаАнотація:
Abstract Shiga toxins (Stxs) are composed of an enzymatically active A subunit (StxA) and a pentameric B subunit (StxB) that preferentially binds to the glycosphingolipid (GSL) globo\xadtriaosylceramide (Gb3Cer/CD77) and to a reduced extent to globotetraosylceramide (Gb4Cer). The identification of Gb3Cer as a tumor-associated GSL in human pancreatic cancer prompted us to investigate the expression of Gb3Cer and Gb4Cer in 15 human pancreatic ductal adenocarcinoma cell lines derived from primary tumors and liver, ascites, and lymph node metastases. Thin-layer chromatography overlay assays revealed the occurrence of Gb3Cer in all and of Gb4Cer in the majority of cell lines, which largely correlated with transcriptional expression analysis of Gb3Cer and Gb4Cer synthases. Prominent Gb3Cer and Gb4Cer lipoform heterogeneity was based on ceramides carrying predominantly C16:0 and C24:0/C24:1 fatty acids. Stx2-mediated cell injury ranged from extremely high sensitivity (CD50 of 0.94 pg/ml) to high refractiveness (CD50 of 5.8 μg/ml) and to virtual resistance portrayed by non-determinable CD50 values even at the highest Stx2 concentration (10 μg/ml) applied. Importantly, Stx2-mediated cytotoxicity did not correlate with Gb3Cer expression (the preferential Stx receptor), suggesting that the GSL receptor content does not primarily determine cell sensitivity and that other, yet to be delineated, cellular factors might influence the responsiveness of cancer cells.
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Dodsworth, Jeremy A., John C. Ong, Amanda J. Williams, Alice C. Dohnalkova, and Brian P. Hedlund. "Thermocrinis jamiesonii sp. nov., a thiosulfate-oxidizing, autotropic thermophile isolated from a geothermal spring." International Journal of Systematic and Evolutionary Microbiology 65, Pt_12 (December 1, 2015): 4769–75. http://dx.doi.org/10.1099/ijsem.0.000647.
Повний текст джерелаАнотація:
An obligately thermophilic, chemolithotrophic, microaerophilic bacterium, designated strain GBS1T, was isolated from the water column of Great Boiling Spring, Nevada, USA. Thiosulfate was required for growth. Although capable of autotrophy, growth of GBS1T was enhanced in the presence of acetate, peptone or Casamino acids. Growth occurred at 70–85 °C with an optimum at 80 °C, at pH 6.50–7.75 with an optimum at pH 7.25, with 0.5–8 % oxygen with an optimum at 1–2 % and with ≤ 200 mM NaCl. The doubling time under optimal growth conditions was 1.3 h, with a final mean cell density of 6.2 ± 0.5 × 107 cells ml− 1. Non-motile, rod-shaped cells 1.4–2.4 × 0.4–0.6 μm in size occurred singly or in pairs. The major cellular fatty acids (>5 % of the total) were C20 : 1ω9c, C18 : 0, C16 : 0 and C20 : 0. Phylogenetic analysis of the GBS1T 16S rRNA gene sequence indicated an affiliation with Thermocrinis ruber and other species of the genus Thermocrinis, but determination of 16S rRNA gene sequence similarity ( ≤ 97.10 %) and in silico estimated DNA–DNA hybridization values ( ≤ 18.4 %) with the type strains of recognized Thermocrinis species indicate that the novel strain is distinct from described species. Based on phenotypic, genotypic and phylogenetic characteristics, a novel species, Thermocrinis jamiesonii sp. nov., is proposed, with GBS1T ( = JCM 19133T = DSM 27162T) as the type strain.
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Yang, Sung-Hyun, Jung-Hyun Lee, Ji-Sun Ryu, Chiaki Kato, and Sang-Jin Kim. "Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments." International Journal of Systematic and Evolutionary Microbiology 57, no. 2 (February 1, 2007): 208–12. http://dx.doi.org/10.1099/ijs.0.64469-0.
Повний текст джерелаАнотація:
A Gram-negative, motile, rod-shaped, psychrophilic bacterium, LT17T, was isolated from deep-sea sediments (3300 m depth) of the East Sea (Sea of Japan). Optimal growth of LT17T requires the presence of 2.5 % (w/v) NaCl, a pH of 7.0–7.5 and a temperature of 17 °C. The isolate grows optimally under a hydrostatic pressure of 10 MPa and growth is possible between 0.1 and <30 MPa. The novel strain is positive in tests for catalase, oxidase, lipase, β-glucosidase and gelatinase activities and reduces nitrate to nitrate. The predominant cellular fatty acids are iso-C13 : 0, iso-C15 : 0, C16 : 0, C16 : 1ω7 and C20 : 5ω3. The DNA G+C content of strain LT17T is 38.8 mol%. Phylogenetic analysis of 16S rRNA gene sequences places this bacterium in the class Gammaproteobacteria, within the genus Shewanella. The closest relatives of strain LT17T are Shewanella japonica (97.8 % gene sequence similarity), Shewanella pacifica (97.5 %), Shewanella olleyana (96.8 %), Shewanella frigidimarina (96.5 %) and Shewanella gelidimarina (95.4 %). The DNA–DNA hybridization levels between the novel isolate and its closest known phylogenetic relatives, S. japonica and S. pacifica, are lower than 14 %. On the basis of this polyphasic evidence, strain LT17T represents a novel species of the genus Shewanella, for which the name Shewanella donghaensis sp. nov. is proposed. The type strain is LT17T (=KCTC 10635BPT=JCM 12524T).
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Wen, Jia, Gilberto Padilla Mercado, Alyssa Volland, Heidi L. Doden, Colin R. Lickwar, Taylor Crooks, Genta Kakiyama, et al. "Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine." Science Advances 7, no. 30 (July 2021): eabg1371. http://dx.doi.org/10.1126/sciadv.abg1371.
Повний текст джерелаАнотація:
Bile salt synthesis, secretion into the intestinal lumen, and resorption in the ileum occur in all vertebrate classes. In mammals, bile salt composition is determined by host and microbial enzymes, affecting signaling through the bile salt–binding transcription factor farnesoid X receptor (Fxr). However, these processes in other vertebrate classes remain poorly understood. We show that key components of hepatic bile salt synthesis and ileal transport pathways are conserved and under control of Fxr in zebrafish. Zebrafish bile salts consist primarily of a C27 bile alcohol and a C24 bile acid that undergo multiple microbial modifications including bile acid deconjugation that augments Fxr activity. Using single-cell RNA sequencing, we provide a cellular atlas of the zebrafish intestinal epithelium and uncover roles for Fxr in transcriptional and differentiation programs in ileal and other cell types. These results establish zebrafish as a nonmammalian vertebrate model for studying bile salt metabolism and Fxr signaling.
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Wang, Li, Liang Chen, Qi Ling, Chen-chen Li, Yong Tao, and Min Wang. "Dyadobacter jiangsuensis sp. nov., a methyl red degrading bacterium isolated from a dye-manufacturing factory." International Journal of Systematic and Evolutionary Microbiology 65, Pt_4 (April 1, 2015): 1138–43. http://dx.doi.org/10.1099/ijs.0.000069.
Повний текст джерелаАнотація:
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, L-1T, which was capable of degrading methyl red was isolated from a dye-manufacturing factory in China. Phenotypic, chemotaxonomic and phylogenetic analyses established affiliation of the isolate to the genus Dyadobacter . Cells occurred in pairs in young cultures but became chains of coccoid cells in old cultures, and produced a flexirubin-like yellow pigment. Strain L-1T could not hydrolyse cellulose, and had a DNA G+C content of 51.3 mol%. The major cellular fatty acids were iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). C16 : 0, iso-C15 : 0 3-OH and C16 : 0 3-OH were the other major fatty acid components. Comparative 16S rRNA gene sequence analysis showed that strainL-1T was most closely related to Dyadobacter fermentans DSM 18053T (99.2 %), Dyadobacter soli JCM 16232T (98.9 %) and Dyadobacter beijingensis CGMCC 1.6375T (98.7 %). However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to JCM 16232T (41.2±1.8 %), DSM 18053T (38.6±2.6 %) and CGMCC 1.6375T (35.0±2.1 %). Strain L-1T could also be differentiated from its closest phylogenetic relatives based on differences in several phenotypic characteristics. These data suggest that strain L-1T represents a novel species of the genus Dyadobacter , for which the name Dyadobacter jiangsuensis sp. is proposed. The type strain is L-1T (DSM 29057T = CGMCC 1.12969T).
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Qiao, Weichuan, Junqi Tao, Yang Luo, Tianhao Tang, Jiahui Miao, and Qiwen Yang. "Microbial oil production from solid-state fermentation by a newly isolated oleaginous fungus, Mucor circinelloides Q531 from mulberry branches." Royal Society Open Science 5, no. 11 (November 2018): 180551. http://dx.doi.org/10.1098/rsos.180551.
Повний текст джерелаАнотація:
In this study, a newly isolated oleaginous fungus, Mucor circinelloides ( M. circinelloides ) Q531, was able to convert mulberry branches into lipids. The highest yield and the maximum lipid content produced by the fungal cells were 42.43 ± 4.01 mg per gram dry substrate (gds) and 28.8 ± 2.85%, respectively. The main components of lignocellulosic biomass were gradually reduced during solid-state fermentation (SSF). Cellulose, hemicellulose and lignin were decreased from 45.11, 31.39 and 17.36% to 41.48, 28.71, and 15.1%, respectively. Gas chromatography analysis showed that the major compositions of the fermented products were palmitic acid (C16:0, 18.42%), palmitoleic acid (C16:1, 5.56%), stearic acid (C18:0, 5.87%), oleic acid (C18:1, 33.89%), linoleic acid (C18:2, 14.45%) and γ-linolenic acid (C18:3 n6, 22.53%) after 2 days of SSF. The fatty acid methyl esters contained unsaturated fatty acids with a ratio of 75.95%. The composition and content obtained in this study are more advantageous than those of many other biomass lipids. Meanwhile, the oleaginous fungus had a high cellulase activity of 1.39 ± 0.09 FPU gds −1 . The results indicate that the enzyme activity of the isolated fungus was capable of converting the cellulose and hemicelluloses to available sugar monomers which are beneficial for the production of lipids.
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Burnatowska-Hledin, Maria, P. Zhao, B. Capps, A. Poel, K. Parmelee, C. Mungall, A. Sharangpani, and L. Listenberger. "VACM-1, a cullin gene family member, regulates cellular signaling." American Journal of Physiology-Cell Physiology 279, no. 1 (July 1, 2000): C266—C273. http://dx.doi.org/10.1152/ajpcell.2000.279.1.c266.
Повний текст джерелаАнотація:
Vasopressin-activated Ca2+-mobilizing (VACM-1) receptor binds arginine vasopressin (AVP) but does not have amino acid sequence homology with the traditional AVP receptors. VACM-1, however, is homologous with a newly discovered cullin family of proteins that has been implicated in the regulation of cell cycle through the ubiquitin-mediated degradation of cyclin-dependent kinase inhibitors. Because cell cycle processes can be regulated by the transmembrane signal transduction systems, the effects of VACM-1 expression on the Ca2+ and cAMP-dependent signaling pathway were examined in a stable cell line expressing VACM-1 in VACM-1 transfected COS-1 cells and in cells cotransfected with VACM-1 and the adenylyl cyclase-linked V2 AVP receptor cDNAs. Expression of the VACM-1 gene reduced basal as well as forskolin- and AVP-stimulated cAMP production. In cells cotransfected with VACM-1 and the V2 receptor, the AVP- and forskolin-induced increases in adenylyl cyclase activity and cAMP production were inhibited. The inhibitory effect of VACM-1 on cAMP production could be reversed by pretreating cells with staurosporin, a protein kinase A (PKA) inhibitor, or by mutating S730A, the PKA-dependent phosphorylation site in the VACM-1 sequence. The protein kinase C specific inhibitor Gö-6983 further enhanced the inhibitory effect of VACM-1 on AVP-stimulated cAMP production. Finally, AVP stimulatedd- myo-inositol 1,4,5-trisphosphate production both in the transiently transfected COS-1 cells and in the stable cell line expressing VACM-1, but not in the control COS-1 and Chinese hamster ovary cells. Our data demonstrate that VACM-1, the first mammalian cullin protein to be characterized, is involved in the regulation of signaling.
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Herrmann, Thomas, Frank van der Hoeven, Hermann-Josef Gröne, Adrian Francis Stewart, Lutz Langbein, Iris Kaiser, Gerhard Liebisch, et al. "Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy." Journal of Cell Biology 161, no. 6 (June 23, 2003): 1105–15. http://dx.doi.org/10.1083/jcb.200207080.
Повний текст джерелаАнотація:
The fatty acid transport protein family is a group of evolutionarily conserved proteins that are involved in the cellular uptake and metabolism of long and very long chain fatty acids. However, little is known about their respective physiological roles. To analyze the functional significance of fatty acid transport protein 4 (Fatp4, Slc27a4), we generated mice with a targeted disruption of the Fatp4 gene. Fatp4-null mice displayed features of a neonatally lethal restrictive dermopathy. Their skin was characterized by hyperproliferative hyperkeratosis with a disturbed epidermal barrier, a flat dermal–epidermal junction, a reduced number of pilo-sebaceous structures, and a compact dermis. The rigid skin consistency resulted in an altered body shape with facial dysmorphia, generalized joint flexion contractures, and impaired movement including suckling and breathing deficiencies. Lipid analysis demonstrated a disturbed fatty acid composition of epidermal ceramides, in particular a decrease in the C26:0 and C26:0-OH fatty acid substitutes. These findings reveal a previously unknown, essential function of Fatp4 in the formation of the epidermal barrier.
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Sravanthi, T., L. Tushar, Ch Sasikala, and Ch V. Ramana. "Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut." International Journal of Systematic and Evolutionary Microbiology 65, Pt_12 (December 1, 2015): 4589–94. http://dx.doi.org/10.1099/ijsem.0.000616.
Повний текст джерелаАнотація:
A Gram-stain-negative spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).
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Vishnuvardhan Reddy, S., S. Aspana, D. L. Tushar, Ch Sasikala, and Ch V. Ramana. "Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar." International Journal of Systematic and Evolutionary Microbiology 63, Pt_6 (June 1, 2013): 2223–28. http://dx.doi.org/10.1099/ijs.0.046292-0.
Повний текст джерелаАнотація:
Two helical-shaped bacteria (strains JC133T and JC143), which stain Gram-negative, were isolated from an alkaline soda lake, Lonar, India. Both strains were obligate anaerobes, mesophilic and required halo-alkaline conditions for growth. Both strains were resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Both strains had phosphatidylglycerol (PG), diphosphotidylglycerol (DPG), glycolipid (GL) and four unidentified lipids (L1–4) as the major polar lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C contents of strain JC131T and JC143 were 58.2 and 58.5 mol%, respectively, and the two strains showed DNA reassociation >85 % (based on DNA–DNA hybridization). Based on the 16S rRNA gene sequence analysis, both strains were identified as belonging to the genus Spirochaeta with Spirochaeta alkalica Z-7491T (99.6 % sequence similarity), Spirochaeta americana ASpG1T (99 %) and other members of the genus Spirochaeta (<93 %) as their closest phylogenetic neighbours. However, strain JC133T and JC143 displayed less than 53.5 % binding (based on DNA–DNA hybridization) with S. alkalica Z-7491T and S. americana ASpG1T. On the basis of physiological, biochemical, chemotaxonomic and molecular properties, strains JC133T and JC143 can be differentiated from other members of the genus Spirochaeta and represent a novel species of the genus Spirochaeta , for which the name Spirochaeta sphaeroplastigenens sp. nov. is proposed. The type strain is JC133T ( = KCTC 15220T = NBRC 109056T).
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Laomeephol, Chavee, Apichai Vasuratna, Juthamas Ratanavaraporn, Sorada Kanokpanont, Jittima Amie Luckanagul, Martin Humenik, Thomas Scheibel, and Siriporn Damrongsakkul. "Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth." Polymers 13, no. 23 (November 29, 2021): 4182. http://dx.doi.org/10.3390/polym13234182.
Повний текст джерелаАнотація:
Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.
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Barajas-Lopez, C., A. L. Peres, and R. Espinosa-Luna. "Cellular mechanisms underlying adenosine actions on cholinergic transmission in enteric neurons." American Journal of Physiology-Cell Physiology 271, no. 1 (July 1, 1996): C264—C275. http://dx.doi.org/10.1152/ajpcell.1996.271.1.c264.
Повний текст джерелаАнотація:
Whole cell recordings were used to investigate the effects of adenosine and several of its analogues on voltage-activated calcium currents (VACC) of myenteric and submucosal neurons. Electrophysiological and pharmacological properties of the soma VACC recorded in myenteric neurons indicate that they are carried through N-type calcium channels, similar to those of the submucosal neurons and to those of the calcium conductance that mediates acetylcholine release at the submucosal ganglia. Adenosinergic compounds inhibited, in a concentration-response and in a voltage-dependent manner, VACC in neurons from both enteric plexuses. The pharmacological profile of the receptors that mediate this effect was similar to that of the receptors involved in presynaptic inhibition in enteric neurons and likely of the A1 subtype. The effects of 2-chloroadenosine (CADO) on VACC were prevented by pretreatment with pertussis toxin (PTX), became irreversible with guanosine 5'-O-(3-thiotriphosphate) (inside the pipette), and were abolished with N-ethylmaleimide (NEM; known to uncouple receptors from G protein complexes). Intracellular recordings were used to further evaluate presynaptic effects of adenosine at the submucosal plexus. Adenosinergic compounds reduced the amplitude of fast excitatory postsynaptic potentials (EPSPs) by acting at nerve terminals. This effect was insensitive to PTX and staurosporine (a protein kinase inhibitor) but was abolished by NEM. CADO effects on EPSPs were not reversed by increasing the extracellular calcium concentration. In conclusion, activation of A1 adenosine receptors inhibits VACC via PTX-sensitive G proteins in myenteric and submucosal neurons. Reduction of cholinergic transmission also involves A1 adenosine receptors and appears to involve the activation of PTX-insensitive G proteins.
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Biswas, Kallol, Amril Nazir, Md Tauhidur Rahman, Mayeen Uddin Khandaker, Abubakr M. Idris, Jahedul Islam, Md Ashikur Rahman, and Abdul-Halim M. Jallad. "A hybrid multi objective cellular spotted hyena optimizer for wellbore trajectory optimization." PLOS ONE 17, no. 1 (January 27, 2022): e0261427. http://dx.doi.org/10.1371/journal.pone.0261427.
Повний текст джерелаАнотація:
Cost and safety are critical factors in the oil and gas industry for optimizing wellbore trajectory, which is a constrained and nonlinear optimization problem. In this work, the wellbore trajectory is optimized using the true measured depth, well profile energy, and torque. Numerous metaheuristic algorithms were employed to optimize these objectives by tuning 17 constrained variables, with notable drawbacks including decreased exploitation/exploration capability, local optima trapping, non-uniform distribution of non-dominated solutions, and inability to track isolated minima. The purpose of this work is to propose a modified multi-objective cellular spotted hyena algorithm (MOCSHOPSO) for optimizing true measured depth, well profile energy, and torque. To overcome the aforementioned difficulties, the modification incorporates cellular automata (CA) and particle swarm optimization (PSO). By adding CA, the SHO’s exploration phase is enhanced, and the SHO’s hunting mechanisms are modified with PSO’s velocity update property. Several geophysical and operational constraints have been utilized during trajectory optimization and data has been collected from the Gulf of Suez oil field. The proposed algorithm was compared with the standard methods (MOCPSO, MOSHO, MOCGWO) and observed significant improvements in terms of better distribution of non-dominated solutions, better-searching capability, a minimum number of isolated minima, and better Pareto optimal front. These significant improvements were validated by analysing the algorithms in terms of some statistical analysis, such as IGD, MS, SP, and ER. The proposed algorithm has obtained the lowest values in IGD, SP and ER, on the other side highest values in MS. Finally, an adaptive neighbourhood mechanism has been proposed which showed better performance than the fixed neighbourhood topology such as L5, L9, C9, C13, C21, and C25. Hopefully, this newly proposed modified algorithm will pave the way for better wellbore trajectory optimization.
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Dahal, Ram Hari, Dhiraj Kumar Chaudhary, Dong-Uk Kim, and Jaisoo Kim. "Hymenobacter polaris sp. nov., a psychrotolerant bacterium isolated from an Arctic station." International Journal of Systematic and Evolutionary Microbiology 70, no. 9 (September 1, 2020): 4890–96. http://dx.doi.org/10.1099/ijsem.0.004356.
Повний текст джерелаАнотація:
A pink-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated RP-2-7T, was obtained from soil sampled at the Arctic station, Spitsbergen, Svalbard, Norway. Cells were strictly aerobic, psychrotolerant, grew optimally at 15–20 °C and hydrolysed CM-cellulose. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RP-2-7T formed a lineage within the family Hymenobacteraceae and clustered with members of the genus Hymenobacter . Its closest relative was Hymenobacter marinus KJ035T (97.6 % sequence similarity). The sequence similarities to other strains were ≤96.9 %. The principal respiratory quinone was MK-7 and the major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The predominant cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), anteiso-C15 : 0, iso-C15 : 0, C16 : 1 ω5c and summed featured 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The DNA G+C content was 62.8 mol%. In addition, the average nucleotide identity and in silico DNA–DNA hybridization relatedness values between strain RP-2-7T and closely related strains were lower than species demarcation thresholds. Based on the resuls of genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain RP-2-7T represents novel species in the genus Hymenobacter , for which the name Hymenobacter polaris sp. nov. is proposed. The type strain is RP-2-7T (=KACC 21670T=NBRC 114391T).
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Merlin, P., J. C. Braekman, D. Daloze, J. M. Pasteels та A. Dejean. "New C26 δ-lactones from the Dufour's gland of the urticating antTetramorium aculeatum". Experientia 48, № 1 (січень 1992): 111–13. http://dx.doi.org/10.1007/bf01923621.
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Shum, Angie M. Y., David C. Y. Fung, Susan M. Corley, Max C. McGill, Nicholas L. Bentley, Timothy C. Tan, Marc R. Wilkins, and Patsie Polly. "Cardiac and skeletal muscles show molecularly distinct responses to cancer cachexia." Physiological Genomics 47, no. 12 (December 2015): 588–99. http://dx.doi.org/10.1152/physiolgenomics.00128.2014.
Повний текст джерелаАнотація:
Cancer cachexia is a systemic, paraneoplastic syndrome seen in patients with advanced cancer. There is growing interest in the altered muscle pathophysiology experienced by cachectic patients. This study reports the microarray analysis of gene expression in cardiac and skeletal muscle in the colon 26 (C26) carcinoma mouse model of cancer cachexia. A total of 268 genes were found to be differentially expressed in cardiac muscle tissue, compared with nontumor-bearing controls. This was fewer than the 1,533 genes that changed in cachectic skeletal muscle. In addition to different numbers of genes changing, different cellular functions were seen to change in each tissue. The cachectic heart showed signs of inflammation, similar to cachectic skeletal muscle, but did not show the upregulation of ubiquitin-dependent protein catabolic processes or downregulation of genes involved in cellular energetics and muscle regeneration that characterizes skeletal muscle cachexia. Quantitative PCR was used to investigate a subset of inflammatory genes in the cardiac and skeletal muscle of independent cachectic samples; this revealed that B4galt1, C1s, Serpina3n, and Vsig4 were significantly upregulated in cardiac tissue, whereas C1s and Serpina3n were significantly upregulated in skeletal tissue. Our skeletal muscle microarray results were also compared with those from three published microarray studies and found to be consistent in terms of the genes differentially expressed and the functional processes affected. Our study highlights that skeletal and cardiac muscles are affected differently in the C26 mouse model of cachexia and that therapeutic strategies cannot assume that both muscle types will show a similar response.